62 results about "Muscle stem cell" patented technology

Compositions and methods for modulating proliferation and / or lineage commitment of stem cells by modulating the Wnt signalling pathways. Modulators of the Wnt signalling pathways and screening methods to identify modulators are also provided. The methods of the invention may be conducted in vitro or in vivo to induce or inhibit stem cell proliferation and / or lineage commitment, and are particularly useful for in vivo stimulation of proliferation and / or lineage commitment of resident stem cells in a tissue.

The present invention is related generally to embryonic-like stem cells isolated from adult human peripheral blood, designated herein as peripheral blood-stem cells (PB-SC), which display the characteristics of embryonic stem cells and hematopoietic cells. These cells have the capability of proliferation and are able to differentiate to other types of cells. These cells are, therefore, suitable for use in stem cell-based therapies, particularly autologous stem cell therapies, for the treatment of various diseases such as neurodegenerative diseases, autoimmune diseases, diabetes, spinal cord damage, multiple sclerosis, cardiovascular disease, stroke and birth defects.

Provided is a muscle stem cell in vitro culture method. A muscle stem cell is cultivated in vitro by using a cell culture medium of a cell factor added with a blood cell or a conditioned medium of the blood cell. Also provided are a culture medium used in the muscle stem cell in vitro culture method and an application thereof.

The present invention relates to the field of muscle regeneration, and more particularly to the replenishment of the in vivo muscle stem cells pool. It more specifically relates to a 5-hydroxytryptamine B1 receptor-stimulating agent, and to a composition comprising said agent, for use as i) a promoter of satellite cells self-renewal and / or differentiation, and / or ii) an agent preventing and / or inhibiting the satellite cells pool exhaustion. The invention further encompasses therapeutic and screening methods.

The invention provides a chemically defined medium for in vitro differentiation of muscle stem cells, that is, a serum-free, more efficient and cheap chemically defined medium for inducing differentiation of muscle stem cells in vitro. Compared with the existing general muscle stem cell differentiation medium, this chemically defined medium can increase the relative expression of MYOG gene by 4.48 times on the second day of differentiation, and increase the relative expression of MYHC gene by 55.28 times on the sixth day of differentiation , the percentage of cell differentiation at the terminal differentiation stage increased from 34.94% to 57.93%, there was a very significant difference, and the induced differentiation formed more, thicker and longer muscle fibers. The invention of the chemically defined differentiation medium further improves the differentiation efficiency of muscle stem cells, provides a more efficient and inexpensive method for muscle stem cells to differentiate into myotubes, and for 3D culture of muscle stem cells to produce cell cultured meat.

Disclosed are methods of genomic modification of skeletal and cardiac muscle using sequence-targeting nucleases and a donor sequence delivered via a virus.

Disclosed are methods of in situ genomic modification of cells (e.g., stem cells, tissue stem cells, muscle stem cells, Sca-1* mesenchymal progenitor cells in skeletal muscle, CD140a* dermal mesenchymal cells) using sequence-targeting nucleases delivered via a virus (e.g., an AAV).