16 results about "Cell bank" patented technology

This invention relates to the interdisciplinary field of cell biology, experimental oncology, and translational medicine, specifically to a cancer-associated fibroblast cell line, its construction method, and its applications. This invention fills the research gap in mouse-derived oral squamous cell carcinoma-associated fibroblast cell line models and provides a method for constructing such a cell line. Through the inverted adherent culture and enzymatic digestion methods of this invention, a mouse-derived oral squamous cell carcinoma-associated fibroblast cell line with a clear origin, high purity, and long-term phenotypic and functional stability can be obtained. This allows for the establishment of a cryopreservable and resuscitable mouse oral squamous cell carcinoma-associated fibroblast cell bank, providing technical support for research related to the tumor microenvironment and immunotherapy.

The present disclosure relates to methods of producing a 900 kDa BoNT / A complex, wherein the method does not comprise a step of precipitation during the purification process. Further provided herein are methods of preparing a Clostridium botulinum working cell bank (WCB).

A method is derived for identifying high affinity monoclonal antibody heavy and light chain pairs from high throughput screens of antibody producing cell libraries such as B-cell and hybridoma libraries. Specifically, the method relates to application of reversed immunocapture and high resolution tandem mass spectrometry for the identification of heavy and light chain pairs of binding antibodies obtained from high throughput screens of antibody producing cell libraries.

This application discloses a muscle stem cell resuscitation and protection washing solution, a quality assessment kit, and a method of use, belonging to the field of cell biology technology. In this application, vitamin E succinate and mannan peptide are used as core antioxidant components, synergistically combined with Gynostemma pentaphyllum polysaccharide, Portulaca oleracea polysaccharide, trehalose, L-proline, and magnesium sulfate heptahydrate to construct a four-in-one resuscitation and protection washing solution that provides isotonic buffering, extracellular protection, intracellular protein stabilization, antioxidant effects, and mitochondrial stabilization. This increases the survival rate of muscle stem cells after resuscitation to over 93%. The kit integrates a multi-dimensional detection system for cell resuscitation and protection washing solution, cell viability, proliferation rate, Pax7 / MyoD double-positive stemness assessment, and cryopreservation damage, achieving closed-loop management of the entire process of muscle stem cell resuscitation, protection, and quality assessment. The double-positive rate of stemness markers reaches over 90%, providing reliable quality assurance for cell bank management and clinical applications.

The present invention relates to a method of assessing the wound healing potency of a mesenchymal stem cell population. In addition, the present invention concerns a method of selecting a mesenchymal stem cell population for producing a stem cell population under cGMP conditions and a method of selecting a mesenchymal stem cell population for producing a stem cell population for subsequent pharmaceutical administration. Further, the present invention relates to a method of selecting a mesenchymal stem cell population for generating a master cell bank and to a method of identifying a tissue suitable as starting material for producing a mesenchymal stem cell population for pharmaceutical use.

The application discloses a rhabdovirus-free Sf9 cell screening method of double pretreated conditioned medium, relates to the technical field of biology, and obtains the rhabdovirus-free Sf9 cell by double pretreatment of the conditioned medium through cross-flow filtration and beta-propiolactone inactivation and hydrolysis, and combination of single cell cloning screening. The method realizes efficient virus interception and high-activity retention of nutritional factors, the single cell cloning formation rate is greater than 40%, and is suitable for virus-free cell bank construction and biological product production.

Provided herein is a method for preparing a sequencing library containing nucleic acids from a plurality of single cells. In one embodiment, the sequencing library contains nucleic acids representing chromatin accessibility from a plurality of single cells. In one embodiment, the nucleic acids include three index sequences. In another embodiment, the present disclosure provides a method for characterizing rare events in isolated cells and nuclei. In an embodiment, providing can include providing a plurality of nuclei or cells in a plurality of compartments, each compartment containing a subset of nuclei or cells or representing a sample.

The present disclosure relates to methods for preparing Clostridium botulinum working cell bank. Further provided herein are methods of producing a composition comprising a 900 kDa BoNT / A complex, said method comprising an acid precipitation step.

Provided is a method for inducing T cells for a cell-based immunotherapy, comprising the steps of: (1) providing Rag 1 and / or Rag 2 gene knockout human pluripotent stem cells bearing genes encoding a T cell receptor specific for a desired antigen, and (2) inducing T cells from the pluripotent stem cells of step (1). Further provided are a cell-based immunotherapy method that uses the T cells for the cell-based immunotherapy and an iPS cell bank for the cell-based immunotherapy.

A method for identifying exogenous viruses based on metagenomic sequencing, comprising the following steps: S1, a data quality control step, filtering the quality of the metagenomic sequencing raw data of the sample to obtain clean sequencing data; S2, a host removal step, aligning the clean sequencing data with a host reference genome and a ribosomal RNA database, removing the host sequences on the alignment, and obtaining enriched microbial sequences. The method integrates kraken2 species identification, RVDB / nt_core database alignment, virsorter2 / checkV verification and other multiple links to form a multi-dimensional identification system, improve the accuracy and reliability of virus identification, introduce a multi-process parallel processing mechanism to support the synchronous analysis of batch samples, greatly improve the analysis efficiency, adapt to the high-throughput detection needs of large-scale cell banks, biological products intermediates and finished products, and solve the problem of low throughput of traditional methods and existing mNGS methods.

The application discloses a culture medium for efficiently amplifying chicken ovary germ stem cells and application of the culture medium to prolong an egg production cycle, and belongs to the cross technical field of livestock breeding and stem cell engineering. The application finds and utilizes an H3K27me3 epigenetic modification inhibitor UNC1999, which is added into a chicken OSCS special culture medium, can effectively reduce the H3K27me3 level in cells, and finally significantly promotes in-vitro proliferation of the OSCS. The culture medium and the culture method can improve the proliferation efficiency of aged chicken OSCS by about 2.4 times within 48 hours. The method breaks through the technical bottleneck of in-vitro amplification of the chicken OSCS, not only provides a new solution for efficiently obtaining high-purity OSCS, but also lays a key technical foundation for directly applying to prolonging an egg-laying period of a laying hen, improving ovary function and establishing a long-term preservation system of avian germplasm resources based on a cell bank, and has great industrial application prospect.

This invention discloses a dynamic risk assessment system and method for cell banks based on graph neural networks. The system includes: a data acquisition layer for real-time acquisition of multi-source heterogeneous data from the cell bank; a dynamic risk assessment engine layer for using a temporal node attention graph neural network to transform the multi-source heterogeneous data into a spatiotemporal graph structure, dynamically analyzing the time-varying graph structure data through an adaptive temporal window and node attention mechanism, and capturing risk propagation paths in conjunction with bioprocess constraints to generate an overall risk score; and a decision execution layer for triggering an alarm process in the quality control module when the overall risk score exceeds a preset threshold, guiding the inventory management system to prioritize the processing of high-risk cell batches, and outputting optimized suggestions for equipment maintenance plans. This invention transforms multi-source heterogeneous data into a spatiotemporal graph structure through a temporal node attention graph neural network and performs dynamic analysis in conjunction with bioprocess constraints to achieve dynamic risk assessment and decision execution for cell banks.

The application discloses a DMSO-free cell cryopreservation solution based on an injection-grade protection system, a use method, related products and application, and belongs to the technical field of immune cell storage. The technical problem to be solved is to provide a cell cryopreservation solution which does not use DMSO and animal-derived components and has higher cell survival rate and higher gamma delta T cell ratio after recovery. The technical solution points are as follows: the cell cryopreservation solution comprises the following components: a colloidal protection component, a cold stabilization component, an antioxidant component, an energy and basic osmotic balance component, an extracellular electrolyte homeostasis regulation component and a transmembrane osmotic pressure regulation component; the osmotic pressure of the cell cryopreservation solution is 280-380 mOsm / kg, the pH of the cell cryopreservation solution is 7.0-7.4; the cell cryopreservation solution does not contain DMSO, animal serum and animal-derived substances. The cell cryopreservation solution is suitable for the storage and transportation of gamma delta T cell treatment products, the storage of an immune cell bank, the cryopreservation of cell treatment before return and the like.

This invention relates to the field of non-destructive testing technology for wind power equipment, and discloses an optimized method, system, equipment, and storage medium for eddy current testing of wind turbine towers. The method includes: acquiring eddy current signals from sample cells and extracting feature vectors to construct a sample cell library containing feature vectors and corresponding parameter labels; dividing the testing area of ​​the wind turbine tower under test into a grid, acquiring the field eddy current signal for each grid, and obtaining a gridded field signal; matching the feature vector of the gridded field signal with the feature vector of defect-free samples in the sample cell library to obtain a defect feature signal; and comparing the defect feature signal with defect calibration samples and paint layer quality grading samples to obtain base material defect detection information and paint layer quality inspection information. This method can be implemented directly using conventional eddy current probes without complex modifications to the testing hardware, facilitating large-scale application in engineering sites for towers with large areas and complex surfaces.

The application discloses a method for establishing a goat bone marrow mesenchymal stem cell line and belongs to the technical field of stem cell culture. The application adopts DMEM / F12 to rinse goat bone marrow to obtain a rinsing product; the rinsing product and complete culture medium are mixed and blown to be a single cell suspension, and then inoculation culture is carried out; after the inoculation culture is completed, subculture is carried out; the subculture method is as follows: when the cell fusion degree reaches 80%, the culture medium is discarded, rinsing, digestion and digestion termination are carried out to obtain a digested cell suspension; the digested cell suspension is blown, centrifuged, precipitated and resuspended, and then inoculated into a new culture bottle; after one hour, the non-adherent cell suspension is transferred to a new bottle, and the adherent BMSCs are reserved for continuous culture, so that the subculture is completed; the main cell bank is established by subculture to the third generation; the aging phenomenon that the proliferation speed of BMSCs is slowed down and the differentiation ability is decreased is effectively delayed, and the differentiation potential stability of the BMSCs is ensured.