29 results about "Leopard" patented technology

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

1. The name of the design product: hourglass (leopard deer). 2. The use of the design product: for timing or decoration. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 2 in use state diagram. 5. The bottom of the design product is not easy to see or not visible during use, omit design 1, design 2 view; It has been embodied in other views, omit design 1, design 2 plan view. 6. Design 1 is designated as the basic design.

This invention belongs to the field of bio-aquaculture technology, specifically relating to SNP loci related to the body color of the leopard-gill spiny perch and their applications. This invention provides SNP loci (16_797851, 13_24322516, and 9_17302939) associated with the body color of the leopard-gill spiny perch. Furthermore, this invention provides a method for mining SNP loci and a method for enhancing the red color of the leopard-gill spiny perch's skin. By employing the HSV color model, the red body color is transformed from a direct sensory representation into a continuous quantitative variable, providing a data foundation for the genetic analysis of body color traits. Based on genome-wide molecular marker screening, genetic markers significantly associated with body color traits are obtained. Combined with the administration of agonists to individuals with genetic advantages, a significant enhancement of the red color of the leopard-gill spiny perch's body surface can be achieved. Therefore, the technical solution of this invention can not only directionally improve the body color trait of the leopard-gill spiny perch and cultivate high-quality new strains with stable body color inheritance, but also promote the development of marine aquaculture and the seed industry.

1. Name of the product in this design: Storage bag (Leopard). 2. Purpose of this design: For storing items. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application provides an indoor breeding method of Plectropomus leopardus, which comprises the following steps: disinfecting an indoor cement pool, laying air bubble stones on the bottom of the cement pool, placing a screen gauze into the cement pool so that the screen gauze adheres to the wall of the cement pool, adding a lighting lamp, adding seawater, and putting Plectropomus leopardus larvae into the cement pool; after the larvae are put into the cement pool, adjusting the water flow speed, feeding fresh and live copepods, and pulling the screen gauze up and down during feeding; stopping feeding the copepods, feeding the compound feed, adjusting the water flow speed, and pulling the screen gauze up and down during feeding; and meanwhile, illuminating 1-3 times per day. The screen gauze is additionally arranged in the indoor cement pool, the cement pool is divided into multiple parts, the feeding domestication and the light domestication are combined with the net pulling and the water flow speed adjusting, the Plectropomus leopardus has good adaptability to the indoor cement pool breeding environment, the growth performance of the Plectropomus leopardus is improved, the rapid growth of the Plectropomus leopardus is promoted, and the breeding cycle of the Plectropomus leopardus is effectively shortened.

The invention discloses a multi-stage purification recirculating aquaculture system for plectropomus leopardus, and relates to the field of fish culture equipment. Comprising the steps that a culture pond body is designed in a partitioned mode to be matched with plectropomus leopardus to inhabit and swim, multi-stage purification treatment modules are arranged in sequence to guarantee clean water quality, a water quality real-time monitoring module collects parameters in the whole area and synchronizes the parameters to a control center, and an intelligent water circulation driving module achieves efficient water circulation through a variable frequency water pump and a pipeline. The dynamic regulation and control module adjusts breeding environment parameters based on monitoring data, the precise feeding module sets a feeding scheme according to feeding habits, the pollution discharge collection and treatment module achieves waste recycling, and the system monitoring and early warning module stores data in a classified mode and conducts early warning in a classified mode. Through multi-stage purification and intelligent regulation and control, the aquaculture water quality is stabilized, diseases are reduced, and the aquaculture survival rate and the product quality are improved; pollution is reduced through precise feeding and pollution discharge design, cost is saved through an energy consumption optimization module, waste recycling meets the environmental protection requirement, and large-scale efficient breeding is assisted.

The present application relates to the technical field of aquatic animal cell culture, and particularly relates to a rapid enrichment and separation method of Plectrypops leopardus gonadal somatic cells. The method comprises the following steps: selecting healthy Plectrypops leopardus for anesthesia; taking gonadal tissue, and after aseptic treatment, cutting, centrifugal washing and filtration, inoculating in a culture medium for primary culture to obtain gonadal cells migrated from the tissue block, and after trypsin-EDTA digestion and centrifugal washing, using 1xPBS containing polysorbate for treatment; after blocking, antibody incubation and washing, verifying the labeling effect by a fluorescence microscope to realize specific enrichment of gonadal somatic cells; after filtration and purification of the enriched fluorescent labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescent positive cells under a fluorescence field, and transferring the cells to a culture dish containing complete culture medium, inoculating the separated fluorescent positive cells in optimized complete culture medium for purification culture, so that the cells adhere to the wall and realize stable subculture.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

1. Name of the designed product: cup cover (leopard). 2. Use of the designed product: cup cover for cups. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The application provides a constraint brown rod bacterium isolated from the intestine of Cephalopholis leopardus and an application thereof, and the preservation number is CGMCC No. 36569. The strain of the application has significant antagonistic effect on Vibrio harveyi, and can be used for preparing products for preventing and controlling aquatic Vibrio diseases. The strain is derived from fish intestine, has good environmental adaptability and host colonization capacity, is high in safety, has no hemolytic activity and carries no drug resistance gene. Feeding experiments show that the strain can promote fish growth, improve feed utilization rate, and enhance the immunity of the body. The application also provides an application of the strain in feed additives or bacterial liquid products, and the application has good popularization value.

The invention belongs to the technical field of biological culture, and particularly relates to an SNP site related to the body color of plectropomus leopardus and application of the SNP site. The invention provides SNP (Single Nucleotide Polymorphism) sites (16797851, 1324322516 and 917302939) related to the body color of the plectropomus leopardus, and the SNP sites are related to the body color of the plectropomus leopardus. In addition, the invention further provides a digging method of the SNP sites and a method for improving the red color of the skin of the plectropomus leopardus. By adopting an HSV color mode, a red body surface color is converted from visual sensory representation to continuous quantitative variables, and a data basis is provided for genetic analysis of body color traits; based on whole genome level molecular marker screening, a genetic marker significantly associated with the body color character is obtained, and a gene dominant individual is fed with an agonist, so that the body surface red color of the plectropomus leopardus can be significantly improved. Therefore, according to the technical scheme, the body color character of the plectropomus leopardus can be directionally improved, a high-quality new strain with stable inheritance of the body color can be cultivated, and the development of the mariculture industry and the breeding industry can be promoted.

1. Name of the product in this design: Tile (Tiger Leopard Faraway). 2. Intended use of this design: for use in ceramic tiles. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The rear view is omitted because it is not visible during use; the right view is symmetrical to the left view, so the right view is omitted; the bottom view is symmetrical to the top view, so the bottom view is omitted.

1. Name of the product in this design: Pendant (Leopard Shadow Fairy Tale). 2. Intended use of this design: for jewelry accessories. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. The top view is shown in other views, so the top view is omitted; the bottom surface of this design product is a part that is not easily seen or cannot be seen during use, so the bottom view is omitted.

The invention relates to the technical field of information, and particularly provides an automatic sorting method for germline stem cells of plectropomus leopardus, which comprises the following steps: selecting healthy plectropomus leopardus as a raw material, anesthetizing with eugenol, taking gonads, cleaning the gonads with a 1 * PBS buffer solution containing antibiotics and an antifungal agent under an aseptic condition, and drying to obtain germline stem cells of plectropomus leopardus; removing surface blood vessels and coating membranes, and cutting into tissue blocks; the method comprises the following steps: re-suspending gonad cells by using a 1 * PBS buffer solution, adding a predetermined volume of suspension into the 1 * PBS buffer solution mixed with trehalose lipid and glycine betaine in a predetermined ratio, incubating, centrifuging, discarding supernatant, and pre-treating the cells to obtain pre-treated cells; putting the separated germline stem cells into a basal culture medium, adding serum, mercaptoethanol, a GlutaMAX additive, a basic fibroblast growth factor, a leukemia inhibition factor and a glial cell-derived neurotrophic factor, and culturing.

1. The name of the design product: hot water bag (leopard pattern PNK). 2. The use of the design product: for hot water bag. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention belongs to the technical field of energy storage, and provides an energy storage system capacity optimization configuration method and device, a medium, a product and equipment. The energy storage system capacity optimization configuration method comprises the following steps: constructing a double-target optimization model taking the life cycle cost and the annual average light abandoning rate of an energy storage system as targets; under a set constraint condition, solving the dual-objective optimization model by using a golden leopard optimization algorithm to obtain the optimal capacity configuration of the energy storage system with the minimum life cycle cost and the minimum annual average light abandoning rate of the energy storage system; introducing crossover operation into the golden leopard optimization algorithm, and in the crossover operation, performing crossover operation on individuals of different populations on the same dimension so as to generate new individuals; and meanwhile, the global search and the local search are balanced according to the absolute value of the escape energy. According to the method, high-proportion new energy can be consumed as a target, and the capacity of the new energy is optimally proportioned.

1. Name of the product in this design: Deep Cultivation Knife Set (Chuanshan Leopard). 2. Purpose of this design: for farming. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the key design points: Design 1 3D view. 5. Design 1 is designated as the basic design.

1. Name of the product in this design: Trunk storage box (Formula Leopard Titanium 3 Ultra). 2. Purpose of this design: For use as a trunk storage box. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention relates to a structure perception and multi-view attention fusion-based tiger identity re-recognition method, which comprises the steps of inputting a to-be-recognized image of an animal with an unknown identity into a feature extraction network, and obtaining a multi-scale fusion feature vector; the feature extraction network comprises a feature pyramid network, a category perception attention module, a structure priori guidance module and a global average pooling and normalization processing module; obtaining a candidate library, wherein the candidate library comprises multi-scale fusion feature vectors of a plurality of animal images with known identities; and calculating the similarity between the multi-scale fusion feature vector of the to-be-recognized image and each multi-scale fusion feature vector in the candidate library, and determining an animal identity re-recognition result of the to-be-recognized image according to the similarity. According to the method and the device, the accuracy and the robustness of individual identity recognition under different shooting view angles, different illumination conditions and complex backgrounds are effectively improved.

1. Name of the designed product: tray (four-leaf clover leopard pattern). 2. Use of the designed product: placing and storing articles. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

1. The name of the design product: insole (LOVE - leopard). 2. The use of the design product: for insoles. 3. The design features of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design features: perspective view.

This invention relates to the fields of medical image processing and artificial intelligence, specifically a deep learning-based method for leopard spot segmentation in wide-angle fundus images. The method includes: constructing a deep learning model for leopard spot segmentation suitable for wide-angle fundus images; performing multi-channel texture enhancement processing on the acquired wide-angle fundus images; inputting the enhanced image into the segmentation model to obtain local leopard spot prediction results; weighted reconstruction of the prediction results to generate a leopard spot probability mask for the entire fundus image, and completing binarization segmentation. The deep learning model uses a multi-source manually labeled fusion mask as a supervision signal and combines a pseudo-supervised optimization mechanism to expand the training data scale under limited manually labeled samples, thereby improving the model's segmentation accuracy. This invention can accurately extract leopard spot regions under complex texture backgrounds, effectively overcoming the problems of low efficiency and strong subjectivity in existing manual interpretation methods, as well as the insufficient segmentation performance of traditional models in wide-angle images.

The application discloses a multi-stage purification leopard grouper circulating water aquaculture system and relates to the field of fish culture equipment; the system comprises a breeding pond body, a multi-stage purification treatment module, a water quality real-time monitoring module, an intelligent water circulation driving module, a dynamic regulation and control module, a precise feeding module and a sewage collection and treatment module; the breeding pond body is divided into zones and is adapted to the habitat and swimming of leopard groupers; the multi-stage purification treatment module is arranged in sequence to ensure clean water quality; the water quality real-time monitoring module collects parameters in the whole area and synchronizes the parameters to a control center; the intelligent water circulation driving module realizes efficient circulation of water bodies through a frequency conversion water pump and a pipeline; the dynamic regulation and control module adjusts breeding environment parameters based on monitoring data; the precise feeding module sets a feeding scheme according to the feeding habits; the sewage collection and treatment module realizes the recycling of waste; and the system monitoring and early warning module stores data in a classified manner and gives graded early warnings. Through multi-stage purification and intelligent regulation and control, the application stabilizes the breeding water quality, reduces the occurrence of diseases, improves the survival rate and product quality, reduces pollution through precise feeding and sewage design, saves costs through energy consumption optimization, realizes the recycling of waste, meets environmental protection requirements and helps large-scale efficient breeding.

1. The name of the design product: hot water bag (leopard bowknot). 2. The use of the design product: for hot water bag. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that can best show the design points: perspective view.

The application belongs to the technical field of aquatic animal breeding and specifically relates to a molecular marker for identifying the genetic sex of Channa maculata x Channa gachua hybrids and application thereof. The application discloses a molecular marker for identifying the genetic sex of Channa maculata x Channa gachua hybrids and application thereof. The marker obtained by the application is a dominant marker, and has the advantages of simple operation, low cost, short experimental period, and the like. The marker can be applied to the scale identification of Channa maculata x Channa gachua hybrids, and male rate data can be obtained.

The present application relates to the technical field of cell separation and culture, in particular to a method for separating and culturing red pigment cells of Plectrypops leopardus.The present application first creates a method for separating red pigment cells of Plectrypops leopardus, and the present application uses EDTA digestion solution to pre-digest epidermal cells on the outer layer of the fin of Plectrypops leopardus, and then uses collagenase digestion solution to digest connective tissue (pigment cells) in the inner dermis, without using trypsin and other digestion enzymes which can cause great damage to cells and other mechanical methods, so that the damage to cells can be reduced, and high-purity red pigment cells of Plectrypops leopardus which are active and can be cultured in vitro can be effectively separated, thereby laying a foundation for subsequent single-cell sequencing, in-vitro cell culture and molecular function verification experiments.

The application discloses a large-scale ferocious beast dynamic monitoring and early warning system and method cooperating with the ground by a UAV, belongs to the technical field of intelligent wild animal monitoring, and is directed to tigers, leopards or bears. The system comprises a data layer, a service layer, an application layer, an intelligent terminal and a user layer which are sequentially connected. The data layer stores full data and is provided with a storage device and an uninterruptible power supply. The service layer provides core services such as AI species identification. The application layer integrates functional modules such as infrared camera intelligent monitoring. The intelligent terminal comprises an infrared trigger camera, a UAV provided with an AI identification confirmation unit and an audible and visual driving device. The user layer accesses the system according to permissions. The method collects data through the infrared trigger camera, triggers hierarchical early warning after AI identification and distance calculation, and links the UAV for patrol and tracking. When necessary, non-injurious driving is started, and the safety of human activities and the protection of large ferocious beasts are considered.

The invention belongs to the field of aquatic organism breeding, and particularly relates to a red body color plectropomus leopardus breeding method based on whole genome selection, which comprises the following steps: obtaining whole genome re-sequencing data of a target group, and carrying out quality control, comparison, variation detection and strict filtration to obtain a high-quality SNP set; based on an R environment / BGLR real model, performing five-fold cross validation evaluation on the density panel of each site; the five-fold average prediction accuracy is used as a performance judgment standard, and the optimal combination of the site density * the model is preferentially determined. The invention provides a special site combination composed of 1000 SNPs, the combination is obtained by sampling chromosomes at equal intervals, and a site ID / coordinate list is listed as follows; in implementation, candidate individuals are subjected to genetic typing by using the 1000 loci, a Bayes B model is input to obtain GEBV, and parent selection and early selection are implemented according to the GEBV.

This invention discloses a method for regulating the natural spawning of leopard-gill sea bass broodstock in an indoor, factory-scale facility, relating to the field of leopard-gill sea bass breeding technology. The invention includes broodstock selection, broodstock rearing, and reproductive regulation of natural spawning. Reproductive regulation of natural spawning refers to controlling conditions such as water temperature, photoperiod, and nutritional enhancement during broodstock rearing to synchronize gonadal development and ultimately induce natural spawning, obtaining a large quantity of high-quality fertilized eggs. This invention can induce broodstock to spawn naturally in indoor rearing ponds within a 45-50 day regulation period, with a spawning period of up to 174 days, achieving a fertilization rate of over 95% and a hatching rate of over 80%. This invention represents a breakthrough in the factory-scale indoor natural spawning of leopard-gill sea bass, effectively promoting the large-scale breeding of leopard-gill sea bass fry and the development of the seed industry.