57 results about "Leopard" patented technology

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The invention provides an automatic bait casting system for training and breeding juvenile plectropomus leopardus, which comprises a basic data module connected with an automatic bait training device and used for acquiring original data of a breeding pond and extracting feeding basic data; the dynamic association module is used for carrying out multi-level cross association modeling, causal inference association, sub-mode mining and classification adaptation to obtain an ingestion-turbidity dynamic association model and outputting an ingestion-turbidity relationship; the hunger index module is used for constructing a hunger index model and outputting the hunger index of each culture pond; the feeding strategy module is used for calculating the next feeding interval, the strip-shaped bait-live bait proportion, the actual time point of next feeding and the bait amount per hour and judging whether extra meal or increase of the next feeding interval is needed or not to obtain a self-adaptive feeding strategy; and the feeding driving module is used for controlling the running action of the automatic bait training device. Scientific feeding can be achieved, the feeding survival rate, the growth rate and the bait training effect are remarkably improved, feed waste is reduced, and intelligent and automatic management of the whole process is achieved.

The invention discloses a method for efficient maturation of ovaries of industrially cultured plectropomus leopardus. The method comprises the following steps: screening healthy plectropomus leopardus individuals from a plectropomus leopardus population as parent fishes; putting the fish in an indoor culture pond for water adaptation to obtain high-quality parent fish; performing induced culture on the high-quality parent fish, wherein the induced culture comprises nutrient enrichment and temperature induction; the temperature induction time is less than or equal to 35 days to promote the ovary maturation of the parent fish. Through an indoor induction mode combining low-temperature energy storage, gradient rewarming and nutrient enrichment feed, the maturation rate of the female plectropomus leopardus ovary can be remarkably increased to 85% or above, and the maturation period is shortened to be within 40 days. The method is suitable for a standardized breeding process of an indoor parent fish breeding workshop, effectively realizes synchronous maturation and quality improvement of the parent fish group ovary, provides a stable parent guarantee for indoor artificial breeding of plectropomus leopardus, and has a good popularization and application prospect.

The invention discloses an indoor detoxification seedling raising method for plectropomus leopardus, and relates to the technical field of biology. The seedling raising method comprises the steps that after an indoor seedling raising pond is sterilized and killed, newly hatched seedlings of plectropomus leopardus are put into the indoor seedling raising pond, and after the newly hatched seedlings open to eat food, different sterilized and killed baits are adopted for feeding the seedlings in a stepped mode for seedling raising culture. According to the method, multiple baits such as nannochloropsis oculata, oyster larvae, SS rotifers, S rotifers, L rotifers, fairy shrimp nauplius larvae, copepod nauplius larvae, copepod middle worms, fairy shrimp adults and copepod adults are fed in a stepped mode, rapid growth of the plectropomus leopardus juvenile fish is promoted, the disease resistance of the juvenile fish is effectively improved, the environment adaptability is enhanced, and the survival rate of the plectropomus leopardus is increased. And the survival rate is well improved.

The invention provides a full-indoor artificial breeding method for plectropomus leopardus, and belongs to the technical field of artificial breeding of marine fishes. The full-indoor artificial breeding method for the plectropomus leopardus comprises the steps of earlier-stage incubation of fertilized eggs, incubation in an indoor breeding pond, breeding in an indoor flowing water pond, breeding in an indoor circulating water pond and the like. By regulating and controlling illumination, the irritability of the fries is greatly reduced, the ecological conditions of the fries are effectively optimized, and the health condition and adaptability of the fries are improved. Meanwhile, different types and different total amounts of biological bait combinations are fed in the larva stage, the juvenile stage and the fish stage of the plectropomus leopardus, transition is made in each bait transfer period, enteritis caused by excessive intake of larvae and juveniles and too little intake caused by inadaptation of bait transfer are effectively reduced, and the problem that the survival rate is low in the bait transfer period can be effectively solved.

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

The utility model discloses an animal abundant claw grinding ball which is mainly used for medium and large animals such as a lion, a tiger, a leopard and the like. A cavity used for containing food is formed in the ball body, and the surface of the ball body is rough. When a large animal makes contact with the surface of the ball body, fingernails of the large animal can be ground by the rough surface, and therefore the claw grinding function is achieved. The device can be used as a toy for large animals to enrich the life interest of the animals and meet the physiological and psychological needs of the animals, so that the engraving behaviors are reduced; meanwhile, food can be placed in the device, the difficulty that the captive animals obtain the food is increased, and the inflexible action behavior of feeding of the captive animals is overcome. And the nail of the captive animal can be polished through the rough surface, so that the claw polishing function is safely completed, meanwhile, the enlarging function is provided for reducing animal carving behaviors, the safety of the captive animal and workers is guaranteed, and the physical and psychological health of the captive animal is also facilitated.

1. The name of the design product: hourglass (leopard deer). 2. The use of the design product: for timing or decoration. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 2 in use state diagram. 5. The bottom of the design product is not easy to see or not visible during use, omit design 1, design 2 view; It has been embodied in other views, omit design 1, design 2 plan view. 6. Design 1 is designated as the basic design.

This invention belongs to the field of bio-aquaculture technology, specifically relating to SNP loci related to the body color of the leopard-gill spiny perch and their applications. This invention provides SNP loci (16_797851, 13_24322516, and 9_17302939) associated with the body color of the leopard-gill spiny perch. Furthermore, this invention provides a method for mining SNP loci and a method for enhancing the red color of the leopard-gill spiny perch's skin. By employing the HSV color model, the red body color is transformed from a direct sensory representation into a continuous quantitative variable, providing a data foundation for the genetic analysis of body color traits. Based on genome-wide molecular marker screening, genetic markers significantly associated with body color traits are obtained. Combined with the administration of agonists to individuals with genetic advantages, a significant enhancement of the red color of the leopard-gill spiny perch's body surface can be achieved. Therefore, the technical solution of this invention can not only directionally improve the body color trait of the leopard-gill spiny perch and cultivate high-quality new strains with stable body color inheritance, but also promote the development of marine aquaculture and the seed industry.

The utility model discloses a multi-layer high-density breeding device for plectropomus leopardus, which comprises a water drainage pipe vertically arranged in the center of a breeding pond and a plurality of inhabiting plates fixedly and vertically arranged on the water drainage pipe at intervals, and the inhabiting plate on the bottommost side is supported on the bottom wall of the breeding pond through a bottom layer supporting column. Every two adjacent inhabiting plates are connected through a supporting rod in a supporting mode, and the diameter of each layer of inhabiting plates is sequentially increased from top to bottom. According to the multi-layer high-density breeding device for the plectropomus leopardus, the rest area of the plectropomus leopardus is enlarged, the breeding density is improved and the breeding benefits are improved by utilizing the original facility drainage pipe in the breeding pond and adding more layers of inhabiting plates under the condition that the area of the breeding pond and the water body are not increased.

1. Name of the product in this design: Storage bag (Leopard). 2. Purpose of this design: For storing items. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application provides a kind of to identify the SSR molecular marker primer group of Guangxi Weizhou Island leopard pattern branchlet puffer original species and application thereof.The application obtains a group of SSR molecular markers capable of identifying Guangxi Weizhou Island leopard pattern branchlet puffer original species: PLWZ-01, PLWZ-02, PLWZ-03, PLWZ-04, PLWZ-05, PLWZ-06, the nucleotide sequences of which are shown in SEQ ID NO.1-6, the SSR molecular markers have the characteristics of high polymorphism but low polymorphism in the original population of Guangxi Weizhou Island;the primer group for amplifying the SSR molecular markers is obtained by screening, and has stable amplification and good repeatability;at the same time, a method for identifying the original species of Guangxi Weizhou Island leopard pattern branchlet puffer is established, which solves the problem that the existing leopard pattern branchlet puffer geographical population is not clear and the original species parent of the seedling of propagation and release is difficult to identify.

The application provides an indoor breeding method of Plectropomus leopardus, which comprises the following steps: disinfecting an indoor cement pool, laying air bubble stones on the bottom of the cement pool, placing a screen gauze into the cement pool so that the screen gauze adheres to the wall of the cement pool, adding a lighting lamp, adding seawater, and putting Plectropomus leopardus larvae into the cement pool; after the larvae are put into the cement pool, adjusting the water flow speed, feeding fresh and live copepods, and pulling the screen gauze up and down during feeding; stopping feeding the copepods, feeding the compound feed, adjusting the water flow speed, and pulling the screen gauze up and down during feeding; and meanwhile, illuminating 1-3 times per day. The screen gauze is additionally arranged in the indoor cement pool, the cement pool is divided into multiple parts, the feeding domestication and the light domestication are combined with the net pulling and the water flow speed adjusting, the Plectropomus leopardus has good adaptability to the indoor cement pool breeding environment, the growth performance of the Plectropomus leopardus is improved, the rapid growth of the Plectropomus leopardus is promoted, and the breeding cycle of the Plectropomus leopardus is effectively shortened.

The invention discloses a multi-stage purification recirculating aquaculture system for plectropomus leopardus, and relates to the field of fish culture equipment. Comprising the steps that a culture pond body is designed in a partitioned mode to be matched with plectropomus leopardus to inhabit and swim, multi-stage purification treatment modules are arranged in sequence to guarantee clean water quality, a water quality real-time monitoring module collects parameters in the whole area and synchronizes the parameters to a control center, and an intelligent water circulation driving module achieves efficient water circulation through a variable frequency water pump and a pipeline. The dynamic regulation and control module adjusts breeding environment parameters based on monitoring data, the precise feeding module sets a feeding scheme according to feeding habits, the pollution discharge collection and treatment module achieves waste recycling, and the system monitoring and early warning module stores data in a classified mode and conducts early warning in a classified mode. Through multi-stage purification and intelligent regulation and control, the aquaculture water quality is stabilized, diseases are reduced, and the aquaculture survival rate and the product quality are improved; pollution is reduced through precise feeding and pollution discharge design, cost is saved through an energy consumption optimization module, waste recycling meets the environmental protection requirement, and large-scale efficient breeding is assisted.

The present application relates to the technical field of aquatic animal cell culture, and particularly relates to a rapid enrichment and separation method of Plectrypops leopardus gonadal somatic cells. The method comprises the following steps: selecting healthy Plectrypops leopardus for anesthesia; taking gonadal tissue, and after aseptic treatment, cutting, centrifugal washing and filtration, inoculating in a culture medium for primary culture to obtain gonadal cells migrated from the tissue block, and after trypsin-EDTA digestion and centrifugal washing, using 1xPBS containing polysorbate for treatment; after blocking, antibody incubation and washing, verifying the labeling effect by a fluorescence microscope to realize specific enrichment of gonadal somatic cells; after filtration and purification of the enriched fluorescent labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescent positive cells under a fluorescence field, and transferring the cells to a culture dish containing complete culture medium, inoculating the separated fluorescent positive cells in optimized complete culture medium for purification culture, so that the cells adhere to the wall and realize stable subculture.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

1. The name of the design product: sandstone block (equation leopard 5). 2. The use of the design product: installed in the front end of the car cover, used to block sandstone insects, interfere with airflow and beautify the appearance. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective drawing.

The invention discloses a robust dynamic direction-of-arrival tracking method and system based on a quantum flower leopard search mechanism and a storage medium, relates to the field of array signal processing, and aims to solve the problem that the existing method cannot solve the problems of poor robustness and huge calculation amount of dynamic direction-of-arrival tracking under impact noise. Comprising the following steps: 1, constructing an expansion fractional low-order covariance according to received data in an impact noise environment; 2, initializing a quantum flower leopard algorithm; 3, determining a global optimal quantum flower leopard position based on a fitness function; 4, updating the position of the quantum flower leopard; 5, updating a local optimal quantum position and a global optimal quantum position; repeating the steps 4-5 until the maximum number of iterations is reached; 6, obtaining new snapshot sampling data, and updating and expanding the fractional low-order covariance matrix and the search space; and 7, repeatedly executing the steps 2-6 until the maximum number of snapshots is reached, outputting a global optimal position, and obtaining a dynamic direction-of-arrival tracking result.

1. Name of the designed product: cup cover (leopard). 2. Use of the designed product: cup cover for cups. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The invention provides a grey matter leopard spot dolomite porosity determination method and a dessert reservoir prediction method. The porosity determination method comprises the following steps: drilling a dolomite plaque area plunger sample with a preset size; performing CT scanning on the plunger sample according to a preset rule to obtain the area of each scanned layer of pores and the area of a dolomite plaque area; utilizing the area of each layer of pores and the area of the dolomite plaque area to obtain the surface porosity of each layer of dolomite plaque area, and obtaining the total porosity of the dolomite plaque area of the plunger sample; calculating the content of dolomite minerals at the corresponding depth position under the well by using a preset first formula in combination with the obtained logging curve data of the plunger sample; and reversely calculating the porosity of the rock core by utilizing the content of the dolomite at the corresponding underground position and the total porosity of the dolomite plaque area in combination with a preset second formula. The porosity value closer to the real porosity can be obtained, and the situation that different porosity values are obtained due to different sampling positions can be avoided.

The application provides a constraint brown rod bacterium isolated from the intestine of Cephalopholis leopardus and an application thereof, and the preservation number is CGMCC No. 36569. The strain of the application has significant antagonistic effect on Vibrio harveyi, and can be used for preparing products for preventing and controlling aquatic Vibrio diseases. The strain is derived from fish intestine, has good environmental adaptability and host colonization capacity, is high in safety, has no hemolytic activity and carries no drug resistance gene. Feeding experiments show that the strain can promote fish growth, improve feed utilization rate, and enhance the immunity of the body. The application also provides an application of the strain in feed additives or bacterial liquid products, and the application has good popularization value.

The invention belongs to the technical field of biological culture, and particularly relates to an SNP site related to the body color of plectropomus leopardus and application of the SNP site. The invention provides SNP (Single Nucleotide Polymorphism) sites (16797851, 1324322516 and 917302939) related to the body color of the plectropomus leopardus, and the SNP sites are related to the body color of the plectropomus leopardus. In addition, the invention further provides a digging method of the SNP sites and a method for improving the red color of the skin of the plectropomus leopardus. By adopting an HSV color mode, a red body surface color is converted from visual sensory representation to continuous quantitative variables, and a data basis is provided for genetic analysis of body color traits; based on whole genome level molecular marker screening, a genetic marker significantly associated with the body color character is obtained, and a gene dominant individual is fed with an agonist, so that the body surface red color of the plectropomus leopardus can be significantly improved. Therefore, according to the technical scheme, the body color character of the plectropomus leopardus can be directionally improved, a high-quality new strain with stable inheritance of the body color can be cultivated, and the development of the mariculture industry and the breeding industry can be promoted.

1. Name of the product in this design: Tile (Tiger Leopard Faraway). 2. Intended use of this design: for use in ceramic tiles. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The rear view is omitted because it is not visible during use; the right view is symmetrical to the left view, so the right view is omitted; the bottom view is symmetrical to the top view, so the bottom view is omitted.

Design represents a bottle for beverages in the shape of a leopard's head in a low-poly technique; the bottle neck finish has a cylindrical shape with a protruding ring-shaped collar, and transitions to the body at almost a right angle; the body represents a three-dimensional shape of a snow leopard's head with eyes, nose, and ears; its surface formed by multiple flat polygonal faces of various shapes and sizes; the bottle bottom has a pentagonal shape; the inner surface of the bottom is recessed with rounded protrusions along the perimeter.

1. Name of this design product: Knife handle (Leopard series). 2. Purpose of the product of this design: The overall purpose of the design is: for cutting objects; the local purpose of the design is: for the handle of a knife. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1. 5. Other circumstances that require explanation: The red covered area in this design product does not belong to the design for which protection is requested for partial appearance.

1. Name of the designed product: brooch (golden leopard hunting). 2. Use of the designed product: for wearing ornaments. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

1. Name of the product in this design: Pendant (Leopard Shadow Fairy Tale). 2. Intended use of this design: for jewelry accessories. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. The top view is shown in other views, so the top view is omitted; the bottom surface of this design product is a part that is not easily seen or cannot be seen during use, so the bottom view is omitted.

The invention relates to the technical field of information, and particularly provides an automatic sorting method for germline stem cells of plectropomus leopardus, which comprises the following steps: selecting healthy plectropomus leopardus as a raw material, anesthetizing with eugenol, taking gonads, cleaning the gonads with a 1 * PBS buffer solution containing antibiotics and an antifungal agent under an aseptic condition, and drying to obtain germline stem cells of plectropomus leopardus; removing surface blood vessels and coating membranes, and cutting into tissue blocks; the method comprises the following steps: re-suspending gonad cells by using a 1 * PBS buffer solution, adding a predetermined volume of suspension into the 1 * PBS buffer solution mixed with trehalose lipid and glycine betaine in a predetermined ratio, incubating, centrifuging, discarding supernatant, and pre-treating the cells to obtain pre-treated cells; putting the separated germline stem cells into a basal culture medium, adding serum, mercaptoethanol, a GlutaMAX additive, a basic fibroblast growth factor, a leukemia inhibition factor and a glial cell-derived neurotrophic factor, and culturing.

1. The name of the design product: hot water bag (leopard pattern PNK). 2. The use of the design product: for hot water bag. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention relates to the field of genetic typing, and discloses a plectropomus leopardus generic genome typing method based on low-cost DNA extraction, which comprises the following steps: carrying out DNA sampling extraction on plectropomus leopardus to construct a re-sequencing library, processing DNA data of all high-depth groups in the re-sequencing library by using Shape software, constructing a haplotype database, and carrying out genotyping on the haplotype database. And performing genotype filling on the low-depth sequencing individuals to obtain haplotypes and genotypes of the low-depth sequencing individuals, and establishing a generic genome IBD cluster typing technology. And finally, generating an individual-level report of the target plectropomus leopardus. According to the invention, a lossless, simple and low-cost DNA extraction technology for the plectropomus leopardus can be established, large-scale sample application is realized, and the purpose of generic genome typing of the plectropomus leopardus is realized.