The invention relates to a method for preparing a bacterial supernatant. The method comprises the following steps: culturing
Pseudomonas environmental strain PF-11 cells; and recovering the supernatant. The invention also relates to a method of reducing the amount of
biofilm on a surface, reducing adhesion of at least one
organism on a surface, reducing small and large area
contamination on a surface, comprising contacting the surface with a supernatant, a supernatant fraction, a modified supernatant or a modified supernatant fraction of
Pseudomonas strain PF-11; or a composition comprising a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of the
Pseudomonas strain PF-11, and one or more acceptable carriers. The invention also relates to a method of killing or reducing the growth of fungi or bacterial cells, or killing or inhibiting the development of insects or marine copepods, comprising contacting the fungi,
bacteria, insects or marine copepods with a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. The invention also relates to a substantially
pure culture of the Pseudomonas strain PF-11. The invention also relates to a culture enriched in the Pseudomonas strain PF-11. The invention also relates to a method of identifying whether a bacterium is capable of producing one or more
extracellular proteases capable of digesting high molecular weight substrates.