76 results about "Coilia" patented technology

The invention discloses a coilia mystus Maillard peptide with a memory improvement function as well as a preparation method and an application of the coilia mystus Maillard peptide. The method comprises following steps: coilia mystus is minced into meat paste, water is added for mixing, protease is added for enzymolysis, and an obtained supernatant is a coilia mystus enzymatic hydrolysis solution; reducing sugar is added to the enzymatic hydrolysis solution and reacts at the temperature of 90-110 DEG C for 1-2 h, a product is taken out and cooled, and coilia mystus Maillard reaction solution is obtained; the coilia mystus Maillard reaction solution is subjected to vacuum concentration and spray drying, and the coilia mystus Maillard peptide is obtained. The biological enzymolysis technology is firstly adopted to release protein peptide with the memory improvement function in coilia mystus protein to the greatest extent, and then the biological efficacy of the protein peptide is further improved through the Maillard reaction. Meanwhile, the Maillard reaction can also improve the flavor of enzymatic hydrolysates, remove fishy smell of marine products and produce pleasant fragrance.

The invention discloses a method for pond culturing Coilia ectenes, which is characterized in that the method comprises the following steps of putting Coilia ectenes in a pond, culturing the Coilia ectenes with the existence of zooplankton, Macrobrachium nipponensis and gobies, wherein the Coilia ectenes forms a balanced biological nutrition chain with the zooplankton, the Macrobrachium nipponensis and the gobies due to the existence quantity of the zooplankton, the Macrobrachium nipponensis and the gobies. With the adoption of the method for pond culturing the Coilia ectenes provided by theinvention, the biological nutrition chain with complementary ecological functions can be established among the Coilia ectenes, the zooplankton, the Macrobrachium nipponensis and the gobies in the aquaculture water; delectable flavor and proper quantity of the in-taken food of the Coilia ectenes can be ensured; the survival rate of the Coilia ectenes can be up to 90%; the Coilia ectanes can be grown above 100 g from about 5 g in two years, thus, a large scale of commercial Coilia ectenes can be formed, and the cultured Coilia ectenes has high qualities, the unsaturated fatty acid content in the meat is up to 6.8% and the collagen content is up to 0.3%. Therefore, the method has a high promotion value.

A coilia ectenes step type breeding method comprises the nine steps of reserved parent pool simulated ecological cultivation, parent pool intensive culture, parent selection for induced spawning, parent induced spawning, natural insemination, germ cell collection, germ cell incubation, indoor intensive fry rearing in a cement pool and large-scale fry culture in an outdoor pool. The method is characterized in that filial-generation coilia ectenes adult fishes which are more than 2 years old and cultured by manpower are used as reserved parents for artificial propagation, and a natural ecology cultivation mode is simulated; the selected parents are transported and placed in an induced spawning pool, after parent induced spawning is conducted, the germ cells are collected, counted and transferred to an incubation pool, after the germ cells are collected, the germ cells are placed in the incubation pool with water, fries open the mouths 5-6 days after membrane rupture is conducted, yolks and freshwater rotifers which are filtered through a 80-mesh silk net are fed to the fries, after the fries are cultivated for 15 days, the fries are divided to different pools, and when the fries are as long as 20-30 mm, the fries are placed to outdoor pools to be cultivated.

The invention relates to a transfer technique of indoor bred Yangtze River Coilia ectenes summer fingerlings, belonging to the field of live fish transportation. The transfer technique consists of steps of net hauling for exercise, net hauling operation and transportation. The transfer technique is characterized in that net hauling for exercise is conducted to the fingerlings before transportation; feeding is forbidden one day before transportation, net hauling operation is conducted to gather the fingerlings in a net enclosure, the fingerlings are scooped into a transportation tool by using a water ladle or a basin and the fingerlings are guaranteed not to leave water during operation; the transportation is divided into short-distance transportation and long-distance transportation according to transportation duration, the duration of short-distance transportation is controlled to be 30-40 minutes, containers for containing the fingerlings for short-distance transportation are plastic barrels, the quantity of fingerlings in each barrel is controlled to be 100-200 and the water temperature is controlled to be 28DEG C; and the duration of long-distance transportation is controlled to be 4-5 hours, the fingerlings in the net enclosure are scooped into a small net cage for temporary culture for 15-30 minutes by using the water ladle after net hauling is conducted, then the fingerlings are contained in bags for transportation, five liters of water and 50 fingerlings are contained in each bag, the bags which contain the fingerlings are contained in black thin film bags to prevent irritation of strong light, and the water temperature is controlled to be below 28DEG C.

The present invention discloses a method for researching age and migration life history of coilia by eardust which belongs to the ichthyological ecology field. The method selects the eardust as researching material, processes grinding to an intersecting surface of the eardust, observes a micro-structure of the eardust for researching age, growth and life history of the coilia. Compared with traditional method, the method provides a new method for researching fish type annual zone, fills blank of unclear bright band and dark lane on the eardust to research fish type age; the eardust grinding has high efficiency, each eardust can be ground only five minutes, and the result is more reliable than other hard bone tissue analyzing; a nail enamel has transparent function to the eardust in embedding and enveloping simultaneity which is more safety and has better transparent effect than using dimethylbenzene. The method is suitable for researching relative age and migration life history of all hard bone fish type with eardust back and belly morphology curve.

The invention discloses a method for cultivating fries of coilia ectenes in a soil pond, comprising the steps of selection of an outdoor soil pond fry cultivation pond, secondary intensified cultivation of parent fishes, separation of the parent fishes and the fries, and early-stage cultivation of the fries of the coilia ectenes by cultivating food organisms including rotifer, cladocera and the like. The method disclosed by the invention is simple and convenient to operate, economical and practical, realizes repeated use of the parent fishes, and is low in fry cultivation cost, high in fry survival rate, and strong in disease resistance. By adopting the principle of natural selection, weak and sick fries are eliminated, so that the purpose of improving the fry quality is achieved. In addition, pelagic organisms are cultivated by utilizing glucose, amino acid and other nutrient solutions, so as to provide sufficient baits for the fries, and thus the quick growth of the fries is guaranteed.

The invention discloses a natural propagation method for coilia ectenes. According to the natural propagation method, parent fishes are bred according to the river and sea migration rule of the coilia ectenes, live baits adaptive to seawater or fresh water living are additionally thrown, pellet feed with vitamin and microelement added is additionally thrown, water flows of a parent fish pool are increased, salinity is regulated and controlled, a breeding fish bed is arranged, fertilization time of fertilized ova is controlled, the fertilized ova is hatched, fish larvas are bred, and fish fries are bred. The bottleneck problems of large-scale breeding of the coilia ectenes and industrialization propagation of the fries are solved, the final spawning rate is up to 98%, the fry obtaining rate is up to 90%, and the fries are strong and healthy.

The invention relates to a distinguishing method for coilia fishes population based on otolith morphology, and belongs to the technical field of aquatic wildlife protection. The method comprises: By collection, photographing, correction, database establishment and discriminant analysis, collecting coilia fish samples in different water areas; after sample collection, carrying the samples back to alaboratory, removing an otolith, and taking photos with a main groove surface facing upward; correcting an amplification factor of all photos according to an unified direction, so that all photos arein the unified direction and amplification factor; establishing a reference coordinate axis system and calculating coordinate point data files of contour end points of the otolith morphology and a main groove inflection point. The analysis method is reliable and has good precision. The method effectively distinguishes different species of coilia fishes, and effectively distinguishes different population of coilia fishes. In addition, an otolith morphological characteristic library and a discriminant function equation of each coilia fish can be established.

The invention discloses a method for selecting specific SRAP (Sequence-Related Amplified Polymorphism) marking tapes for coilia ectenes genders and a gender determination method. The specific SRAP marking tapes for coilia ectenes genders are selected through SRAP-PCR reaction of 49 primer sets; and then the DNA sample of the coilia ectene which is not clear in gender is amplified through a specific primer set; and the gender of the coilia ectenes is differentiated according to whether the corresponding specific marking tapes can be amplified or not. The method provided by the invention overcomes the limits and shortcomings of the existing coilia ectenes gender differentiation technology, and therefore, an accurate and convenient gender differentiation method is provided.

The invention discloses a mixed feed for lobsters. The mixed feed comprises, by weight, 20-35 parts of fish meal, 15-20 parts of Coilia ectenes Jordan, 5-8 parts of meat and bone meal, 15-20 parts of flour, 8-10 parts of corn, 11-15 parts of soybean meal, 4-8 parts of rapeseed meal, 2-4 parts of fish oil, 10-15 parts of corn protein powder, 1-3 parts of pumpkin seed powder, 4-6 parts of sweet rice flour, 0.1-0.3 parts of choline, 2-3 parts of edible salt, 3-6 parts of grease, 2-5 parts of bentonite, 0.1-0.3 parts of an immunopotentiator, 0.1-0.3 parts of a liver protection agent, 0.1-0.2 parts of multiple vitamins, 0.1-0.2 parts of multiple mineral matters and 1-2 parts of calcium dihydrogen phosphate. Compared with feeds in the prior art, the mixed feed for lobsters, provided by the invention, has the advantages of abundant sources of raw materials, enhancement of the disease resistance and the immunity of lobsters due to inclusion of drug components, high weight gain rate of the lobsters, small dosage, and high survival rate of the lobsters.

The invention discloses artificial propagation and domestication methods of coilia ectenes, relating to a propagation and domestication method of fishes. The artificial propagation method comprises the steps of parent fishing, artificial insemination and incubation in a laboratory; and the domestication method comprises the steps of: 1. putting coilia ectenes fries of uniform size in a glass jar with water, wherein the main feeding bait is composed of 10 kinds of bait such as a compound feed, rotifers and the like, the average water temperature in the feeding period is 25 DEG C; the content of dissolved oxygen is higher than 6 mg/L, the content of ammonia nitrogen is lower than 0.14 mg/L, and the bait composite is fed four times every day; and 2. using the fish at the first stage as materials and continuing to feed the fish for 60 days, wherein the density of released is 50 fish per square meter, the same artificial feed is used in the period, and the feeding condition and temperatureare same. Therefore, mass propagation of coilia ectenes is ensured, the living rate is high, the weight of artificially domesticated coilia ectenes is increased, and the requirement of economical production is met.

The invention provides a primer for identifying coilia ectenes and coilia brachygnathus and a molecular biological method. The primer contains an amplified primer group and an extended primer group. A nucleotide sequence of the amplified primer group is shown as SEQ ID NO:1-16, a nucleotide sequence of the extended primer group is shown as SEQ ID NO:17-24. The primer combination can be effectively used for identifying the coilia ectenes and the coilia brachygnathus. The molecular biological method comprises the steps that a to-be-tested coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequencing library is established, sequencing is conducted on the library to obtain a sequencing result; based on the sequencing result, a coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequence is determined; then Structure2.3.2 software is utilized to draw a column diagram of population genetic differences and a column diagram of individual genetic differences, and species are judged by utilizing analysis. The method includes simple and convenient steps and is accurate, efficient and low in cost.

The invention relates to a method for drawing blood from coilia ectenes under anaesthesia, and the operation steps are as follows: firstly adding mixed water of pond water and deep well water into a container, regulating the water temperature to 10-20 DEG C, and adding edible salt and tricaine sulfonate into the mixed water for preparing anaesthetic solution, wherein the salt degree is 5-7 per mill and the percentage concentration of the tricaine sulfonate is 0.01%-0.03%. The coilia ectenes is placed in the anaesthetic solution, and the anaesthetic time is 2-4 minutes. A syringe is used for drawing the blood, the part is located at the bottom of the spine above the anus, the time for drawing the blood is 1-1.5 minutes, and the coilia ectenes after drawing the blood is transferred into a temporary cultivation pond for regaining consciousness. The method can successfully carry out anaesthesia and blood drawing on the coilia ectenes against the physiological characteristics of the coilia ectenes, the method is simple, the cost is low and the volume of drawing the blood is large.

The invention discloses a molecular marker for identifying different ecotypic species of coilia ectenes. Polymorphic loci of retrotransponsons SINE of coilia ectenes are selected as targets, and a specific primer is designed according to flanking sequences inserted into the loci; DNA segments of the SINE insertion or deletion loci are obtained by applying the methods such as PCR amplification, agarose gel electrophoresis, molecular cloning and the like, and different species of the coilia ectenes are identified by using the specific DNA segments. By adopting the molecular marker, the species of the coilia ectenes can be quickly and accurately determined, and the molecular marker has extensive use in the aspects such as molecular marker-assisted breeding of the coilia ectenes, artificial discharge effect assessment of fries, dynamic species monitoring and the like.

The invention provides a juvenile coilia ectenes feed additive and a preparation method. The additive is prepared from the following raw materials: chitosan, digestive enzyme, carriers, lysine, glycine, glycine betaine, tea polyphenol, organic selenium, vitamin C, fish oil, emulsifying agent, sodium alginate, gelatin and citric acid. The preparation method comprises the following steps: preparing the digestive enzyme into a digestive enzyme solution, adsorbing with the carriers, then adding the sodium alginate, the gelatin and the citric acid into water, heating, preserving the heat, adding the emulsifying agent while stirring to obtain a mixed solution, adding the digestive enzyme carrier solution, the chitosan, the lysine, the glycine, the glycine betaine, the tea polyphenol, the organic selenium, the vitamin C and the fish oil into the mixed solution, homogenizing at the high pressure, spray drying, and preparing the feed additive. The additive provided by the invention provides sufficient nutrition to the juvenile coilia ectenes, can increase the survival rate of the raised juvenile coilia ectenes, capable of enabling the juvenile coilia ectenes to have the raising value, capable of improving the fertilizer efficiency and capable of decreasing the feed coefficient.

The invention discloses an asplanchna eliminating method for early-stage indoor Yangtze river coilia ectenes seedling culture. The method is characterized in that during early-stage culture of coilia ectenes seedlings which are 12-18 days old, when asplanchna multiple rapidly and grow, salt is increased step by step until the salinity reaches 5, after the salinity reaches 5 through salt increasing, stabilization is conducted for 48 hours, during salinity stabilization, brackish water with the salinity of 5 is adopted for water changing, and asplanchna which sink to the bottom are eliminated in time; during asplanchna elimination through salt increasing, feeding with brachionus angularis and brachionus calyciflorus is stopped, and switching is conducted with brachionus urceus which are salvaged and collected with a bolting-silk net of 150 meshes and filtered with a bolting-silk net of 80 meshes; after 95% of the asplanchna sink to the bottom, salt is decreased step by step, pure water is adopted as salt decreasing water, and in the salt decreasing process, bottom suction is conducted once in the morning and afternoon respectively everyday to further eliminate the sunken asplanchna thoroughly; two days after step-by-step salt decreasing, the salinity of water in a seedling culture pond drops to 2 or below, and all original operations of seedling culture are restored.