48 results about "Scatophagus argus" patented technology

The invention discloses a combined method of a stereo ecological polyculture mode and the prevention and control of hepatopancreatic necrosis disease and white spot syndrome. Specifically, 3 days before adding prawn seeds, 200 scatophagus argus fries with length of 3-5cm and 300 siganids fries with length of 2-3cm are added into every Mu of a breeding pond; when prawn seeds are to be added, a prawn seed addition amount is 80000-100000 seeds/Mu; when prawn length reaches 4-5cm, 5-6 Nile perch with length of 8-10cm and 10-15 hybrid giant tiger groupers with length of 8-10cm are added into every Mu of the pond. According to the invention, fishes with different feeding habits are cultured with prawns at a same time, such that diseases and weak prawns are eliminated in time, prawn disease infection source is eliminated, and a water cleaning function is realized. A polyculture mode of prawns and fishes with suitable specifications is started from a breeding early-mid stage, such that a prawn disease infection source is cut off, and hepatopancreatic necrosis disease and white spot syndrome outbreak is prevented. The breeding mode is optimized, such that prawn breeding success rate is improved. The method provided by the invention is suitable for the breeding of prawns such as litopenaeus vannamei, marsupenaeus japonicas, penaeus monodon and the like.

The invention relates to the field of aquaculture, in particular to an indoor high-density breeding method of current-year fingerlings of scatophagus argus. The indoor high-density breeding method consists of breeding preparation, fingerling pulling net counting, fingerling transportation and delivery, bait management, water quality management and pulling net capture and adopts an indoor cement pool for high-density breeding of the current-year fingerlings of the scatophagus argus; aquaculture water is estuary brackish water, artificially-bred fingerlings that are converted to eel feed are transported to the indoor cement pool, the specification body length is 1.3-1.5 cm, and the breeding density is 300-400/m<2>; the eel feed is fed to the fingerlings with a respective body length of lessthan 3 cm, and floating pellet feed is fed to the fingerlings with a respective body length of 3 cm; during breeding, sewage suction and bottom cleaning are performed on time, and water change and pool turnover are performed timely; as for the current-year fingerlings with a respective specification body length of more than 4-5 cm, the pulling net capture can be performed to transfer to the pool for breeding. The indoor high-density breeding method provided by the invention has the beneficial effects that the survival rate after indoor high-density breeding for 181 days is 80-85 percent, and the technical blank of indoor intensive and large-scale production of the current-year fingerlings is filled up.

The invention relates to a method for scatophagus argus reproduction regulating and total artificial breeding. The method invention is characterized by comprising the step of adding ecbolic dilacor into odinagogue, wherein the ecbolic dilacor comprises the components by weight percent: 40-50% of cholesterol, 20-30% of propylene glycerol alcohol ester and 20-30% of olein. The invention further provides the ecbolic dilacor used in the scatophagus argus reproduction regulating and total artificial breeding method. The invention has the advantages that: the aims of synchronously maturing the parent and improving the gametogamy quality are achieved by the technology for acclimatizing the wild parent fish of the scatophagus argus, intensifying the nutrition during breeding the parent, regulating the physical-chemical parameter of the water quality, and slowly releasing the hormone; and the synchronous release of the male gametogamy and the female gametogamy is realized, a stable reproduction regulating technology and a high-efficiency scatophagus argus artificial breeding technology are built by the high-efficiency artificial odinagogue and a total artificial ecbolic technology.

The invention relates to a scatophagus argus and macrobrachium rosenbergii intercropping overwintering mode. The scatophagus argus and macrobrachium rosenbergii intercropping overwintering mode adopts a macrobrachium rosenbergii overwintering pond and is characterized in that scatophagus argus is intercropped in the pond; before stocking, the interior of the pond is washed and cleaned; an inflation head is placed in the pond for continuously inflating, effective water level is 1m, and a transparent plastic film covers the entrance of the pond; fresh water is adopted, subjected to primary precipitation by virtue of the pond and secondary precipitation by virtue of a reservoir and preheated and then is applied in the pond; a circular heating hot water pipe is also paved at the bottom of the overwintering pond, a water inlet pipe in the pond is sheathed by a 60-mesh bolting silk mesh for filtering, water is changed every 7-10 days, water changing temperature difference is controlled to be +/-1 DEG C, temperature is controlled to be 20-22 DEG C and synchronously raised at a rate of 0.5 DEG C per day before breeding of macrobrachium rosenbergii, the temperature is stabilized for 15-20 days when the temperature is raised to 25 DEG C, and then the temperature is raised for a second time until the temperature is raised to 29 DEG C; the pond is reversed once every month; bait is thrown once in the morning and evening respectively, compound bait is adopted for feeding in the morning, fresh and alive trash fish meat paste or snail meat is used for feeding in the evening, and a scatophagus argus extruded feed is supplemented; and pollution is absorbed once everyday, and water quality indexes comprise that dissolved oxygen is more than 5mg/L and pH value is 7.5-8.5.

The invention discloses a method for artificially reproducing scatophagus argus. The method includes rearing female and male parent fish in ecological simulation modes; inducing the maturity of sexualglands of the parent fish by the aid of ambient salinity stimulation and spawning induction hormone injection; carrying out artificial fertilization and incubation; rearing scatophagus argus fry. Themethod has the advantages that scatophagus argus artificial reproduction technical gaps can be filled by the aid of the method, the problem that existing scatophagus argus cannot naturally spawn under artificial culture conditions can be solved by the aid of the method, and excessive dependency of the scatophagus argus culture industry on natural resources can be relieved.

The invention relates to an in vitro culture method for scatophagus argus kidney cells. The culture method comprises the following steps of: (1) preparing an L-15 basic culture medium, a primary culture medium, a basic fibroblast growth factor (bFGF)-containing primary culture medium and a subculture medium; (2) performing primary culture on the scatophagus argus kidney cells: a, acquiring a sterile kidney, b, digesting and separating the kidney cells, and c, inoculating; and (3) performing subculture on the scatophagus argus kidney cells. According to the invention, the adopted scatophagus argus kidney cell culture method is strong in repeatability and good in effect; and by reference and improvement on the conventional saltwater fish cell culture method, the culture method suitable for scatophagus argus kidney cell culture is groped, a stable kidney cell culture system is established, a scatophagus argus kidney cell line is established for the first time, a cell model is expected to provide for osmotic pressure regulation of scatophagus argus, and deep research of functional genomes of the scatophagus argus is facilitated.

Provided is a mixed culture method for scatophagus argus and macrobrachium rosenbergii in a land-based greenhouse cement pool. The method is characterized in that the scatophagus argus and macrobrachium rosenbergii are cultured in a mixed mode in a land-based greenhouse cement pool; when the length of macrobrachium rosenbergii grows to 3 to 4 cm, the scatophagus argus is released; in the early culture stage, the gable of the greenhouse is enclosed, doors are kept close after use, and no shading film is applied; in the middle culture stage, the gable and the doors are opened for ventilating and half-warehouse shading film is used; in the later culture stage, the gable is closed and doors are kept close after use, the half-warehouse shading film is kept; water is changed once a week and each time one half water is changed; in the middle culture stage, if the water quality deteriorates due to the emergence of large quantity of filamentous algae and enteromorpha, sediment upturning is carried out for one time, wherein the sediment upturning is carried out by fishing net hauling and drainage bottom cleaning. Whole pool feeding is adopted with compound feed for macrobrachium rosenbergii twice a day, no feed for scatophagus argus is used; when the water temperature reaches 18DEG C, catching and harvesting are carried out. when harvesting, the weight of scatophagus argus is increased by 50g, which reaches the standard for sale; the scatophagus argus and the macrobrachium rosenbergii can be used as reserved parents for overwintering culture.

The invention provides a hormone kit for scatophagus argus spawning induction and an artificial spawning induction and insemination method for obtaining scatophagus argus fertilized eggs in batches, which belong to the technical field of aquaculture. The hormone kit for scatophagus argus spawning induction comprises gonadotropin releasing element A4, doxolone maleate and human chorionic gonadotropin which are independently subpackaged. Based on the hormone kit, scatophagus argus is subjected to artificial spawning induction by adopting a three-needle method, so that the problems of long effecttime, female scatophagus argus stagnation, high difficulty in artificial spawn extrusion after spawning induction, egg mass and gonad degeneration are effectively solved. The artificial inseminationmethod is adopted, and the problems that natural spawning eggs of scatophagus argus are over-mature or collapse in vivo, the influence of the environment is large, the spawning induction success rate,the fertilization rate and the hatching rate are extremely low, and fertilized eggs are difficult to obtain in batches are effectively solved.

The invention belongs to the field of aquaculture and particularly relates to a breeding method for scatophagus argus. The breeding method is characterized in that scatophagus argus bred in a pond isselected as parent fish which is subjected to five links of conventional cultivation, strengthened cultivation, artificial spawning, artificial incubation and seed rearing, so that a rate of inducingspawning can reach 100%, a fertility rate is 90% or more, and a hatchability rate of fertile eggs is 93%. The breeding method successfully builds a totally artificial breeding technology of the scatophagus argus, and can be used for large-scale production and breeding of the scatophagus argus.

The invention relates to the technical field of scatophagus argus culture, and provides a indoor larva and juvenile fish cultivation method for scatophagus argus in a cement pond. The method comprisesthe eight steps of fry distribution, water temperature control, salinity regulation and control, illumination, dirt absorption, water change, tank turning culture and bait feeding. A bait sequence and a feeding method for the young scatophagus argus are established, and the technical problem of first feed of scatophagus argus fries is solved; and the technical parameters of the cultivation waterenvironment of the young scatophagus argus are determined, a temperature control and salt regulation technology is adopted, the growth efficiency and the cultivation survival rate of the young scatophagus argus are improved, and the blank in the field of artificial cultivation of young scatophagus argus is filled.

The invention discloses a method for controlling prawn hepatopancreas necrosis by polyculture of scatophagus argus and marsupenaeus japonicas. The method comprises: in breeding, throwing marsupenaeus japonicas shrimp seeds whose total length is 0.8-1.0 cm, throw-in density being 70-80 thousands seeds in each mu, when the shrimp seeds growing to 2-3 cm of marsupenaeus japonicas body length, throwing scatophagus argus whose body length is 3-5 cm, throw-in volume being 200-300 fish/mu. Through throwing the scatophagus argus which has omnivory and benthic feeding habit in proper density, proper specification, prawn culture pond water body and bottom pollution can be reduced, so as to improve culture pond environment, reduce harmful bacteria contents in the pond water and bottom mud, and control occurrence and diffusion of bacterial diseases in prawn culture. The method can effectively eliminate redundant nutrient substances in the bottom of the pond and in the water, reduces bacteria number, prevents outburst of prawn hepatopancreas necrosis, and improves success rate of culture.

The invention relates to a method for quickly identifying oplegnathus punctatus and scatophagus argus. The method comprises the steps of (1) extracting genome DNA from oplegnathus punctatus samples and scatophagus argus samples; (2) performing PCR amplification through a specific primer; and (3) performing gel coagulation and electrophoresis on PCR amplification products, taking a photograph, andrecording a comparison electrophoresis result, wherein the oplegnathus punctatus has a clear specificity identifying strip at 160bp, and the scatophagus argus has a clear specificity identifying stripat 200bp. The method disclosed by the invention is accurate, simple and quick to identify, low in price, low in requirements for instruments and equipment, suitable for large-scale identification operations and has favorable application prospects.

The invention discloses application of a scatophagus argus vitellogenin gene to detection of water area environmental hormones. The application comprises the following steps: obtaining a VTGAb full-length sequence according to a RACE method; sampling and extracting liver RNA (Ribonucleic Acid) after injecting estrogen into scatophagus argus in a gradient manner; detecting expression of VTGAb by adopting an RT real-time quantitative PCR (Polymerase Chain Reaction) method; taking EF1-a as an internal control gene and carrying out absolute quantification on cDNA (complementary Deoxyribonucleic Acid) of liver tissues to establish a detection system of a VTGAb transcriptional level; putting scatophagus argus which is not sexually mature into a water body to be detected and sampling and extracting the liver RNA; detecting expression amount of the VTGAb; and comparing with the detection system, and analyzing the pollution degree of the environmental hormones of a water sample. By adopting the application disclosed by the invention, the pollution condition of the environmental hormones can be measured under the condition that the economic loss is relatively small and the single variable of the scatophagus argus is controlled; and the scatophagus argus vitellogenin gene can be used for the pollution degree of the environmental hormones of water samples of seawater, the mouth of a river and a saline water internal lake.

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.

The invention discloses a specific molecular marker for identifying genetic sex of scatophagus argus as well as primers and application of the specific molecular marker. The molecular marker comprisestwo DNA fragments which are partially homologous in X chromosome and Y chromosome of the scatophagus argus, a nucleotide sequence of the DNA fragment partially homologous in the X chromosome is as shown in SEQ ID NO.1, and a nucleotide sequence of the DNA fragment partially homologous in the Y chromosome is as shown in SEQ ID NO.2. The two DNA fragments partially homologous in the X chromosome and the Y chromosome of the scatophagus argus are screened out from scatophagus argus genome information, and specific primers are designed for identifying the genetic sex of the scatophagus argus, so that the genetic sex of the scatophagus argus can be quickly and accurately distinguished. The method is suitable for accurately identifying the genetic sex of the scatophagus argus in a laboratory oran aquaculture enterprise, can also be used for identifying and screening the sex of the scatophagus argus in different growth stages and different groups, and has important significance in researching a sex determination and differentiation mechanism of the scatophagus argus and controlling the sex of the scatophagus argus.

The invention provides a bait domestication method for young sea horses. The bait domestication method comprises the four steps of preparation of a breeding pond, ecological construction of a domestication pond, installation of domestication equipment and management in the domestication period. 5 days before the juvenile sea horses enter the culture pond, the culture pond is prepared, a culture net cage and a bait table are placed, scatophagus argus and horseshoe shells are put into the culture pond, and 0.3-0.5 ppm of shrimp slices are poured into the culture pond every day until the water body is tawny. After the breeding ecology is constructed, feeding the young sea horses three times a day according to the standard that 200 young sea horses are fed into each breeding net cage, feeding the young sea horses in a satiation mode, and thoroughly cleaning a bait table 1.5 h after feeding each time. And domesticating for about 5 days until the hippocampus can actively eat opossum shrimps on the bait platform, and after domestication is finished, transferring the hippocampus into a culture pond for culture. According to the method for domesticating the ecological sea horse bait, an ecological breeding system is constructed, the breeding water body is stable, the domestication efficiency is high, water is saved, and the labor cost is reduced.

The invention provides a factory-like artificial breeding method for scatophagus argus. Scatophagus argus factory-like artificial breeding is achieved through fishpond laying, water quality management, putting density, feed feeding and ecological environment construction, the goals of rapid growth and high survival rate of scatophagus argus are achieved, the survival rate is higher than 90%, and the average weight of scatophagus argus fry bred for 5 months is higher than 290 g.

The invention discloses a method for desalination culture of scatophagus argus. Due to seawater temporary culture and rapid gradient desalination treatment, the desalination speed is minus 2%./1h, thesurvival rate obtained after complete desalination reaches 92.7%, and meanwhile the rapid growth advantage is shown for desalination seedling culture. According to the method for desalination cultureof scatophagus argus, desalinated scatophagus argus fry can be directly subjected to desalination culture, the culture cost is greatly lowered, diversified efficient desalination of seawater fish isachieved, meanwhile, an important approach is supplied to energy-saving low-carbon green development industry structure upgrading for the culture variety, inland development of scatophagus argus culture is achieved, diversified culture modes are achieved, and the culture scale is enlarged.

The invention discloses anti-aging pre-baked bread containing composite collagen anti-freeze peptide and plant hydrophilic colloid and a preparation method of the bread. The preparation method comprises the steps that 1, yeast is activated at constant temperature; 2, plain flour, ask gum, tamarind gum, scatophagus argus fishskin anti-freezing polypeptide powder and salmon fishskin anti-freezing polypeptide powder are evenly mixed; 3, liquid ferment and the mixture obtained in step 2 are mixed with water and stirred to be soft until a gluten film appears; 4, sodium chloride and a yeast water solution are added and stirred to obtain dough; 5, after primary standing, exhausting, relaxation and secondary standing are carried out on the dough obtained in step 4, the dough is cut into blocks orshaped in a specific shape; 6, fermenting is conducted; 7, a pre-baked bread semi-finished product is obtained; 8, the anti-aging pre-baked bread containing composite collagen anti-freeze peptide andplant hydrophilic colloid is obtained. On the one hand, mechanical damage of ice crystals to the pre-baked bread is reduced, and therefore the anti-aging capacity of the bread is improved; on the other hand, the stability of the net structure of gluten is improved, and the strength of the gluten is improved.

The invention relates to an efficient artificial intensified cultivation method for scatophagus argus parents. The method comprises the technical means of cultivation pond selection, parent selection, water quality regulation and control, bait feeding, male and female separate cultivation, hormone ripening acceleration and the like, the cultivated scatophagus argus parent is high in maturation rate, male and female gonad development is synchronous, and a guarantee is provided for scatophagus argus large-scale breeding.