Money fish luteinizing hormone lh gene, money fish lh recombinant protein and application

Abstract

The invention discloses scatophagus argus luteinizing hormone (LH) gene, scatophagus argus LH recombinant protein and application, in particular to the scatophagus argus LH recombinant protein obtained from the scatophagus argus luteinizing hormone (LH) gene through inducible expression of a prokaryotic expression system, and application of the scatophagus argus LH recombinant protein; the cDNA sequence overall length of the scatophagus argus luteinizing hormone (LH) gene is obtained, and the scatophagus argus LH recombinant protein is also obtained through prokaryotic expression; the pure LH recombinant protein is obtained through purification of an affinity column, and can be applied to in-vitro injection of fish to promote gonad development of the fish, and further the gonad development of the fish can show synchronism.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a cDNA sequence of a money fish luteinizing hormone LH gene, and the purification and application of a money fish LH recombinant protein obtained by constructing a recombinant plasmid and inducing expression of a prokaryotic expression system. Background technique [0002] Scatophagus argus, also known as golden drum fish, belongs to Perciformes (Perciformes), Scatophagidae (Scatophagidae), and is distributed in the East my country Sea and South China Sea. It is a high-quality economic fish with ornamental value. Moneyfish is a euryalline fish, which is distributed in sea areas with extremely high salinity, and can grow and survive well in pure fresh water. It can be directly transferred from 35‰ sea water to fresh water without desalination and domestication. Its rare adaptability to salinity makes it very suitable as a material for studying the reproducti...

AI Technical Summary

Problems solved by technology

[0006] Problems existing in the existing technology: With the continuous expansion of the scale of aquaculture, in order to ensure the stable production mode of the quantity and ensure the efficiency of reproduction and growth, many drugs are put into the production industry, which will undoubtedly cause certain harm to the environment and the human body

Since the development of germ cells requires a strict special environment, the lack of in vitro culture models has restricted the study of the molecular mechanism of spermatogenesis for a long time.

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