Method for scatophagus argus reproduction regulating and total artificial breeding
A technology for artificial reproduction, money fish, used in fish farming, climate change adaptation, animal feed, etc.
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Embodiment 1
[0029] Embodiment 1 parental cultivation and ripening technology
[0030] From January to March every year, the male and female broodstock are intensively cultivated. Based on the research on the nutritional requirements of the broodstock, vitamins A and E, and highly unsaturated fatty acids can significantly improve the gonad development of the broodstock and promote the further maturity of the male and female broodstock. Strengthen the regulation of physical and chemical parameters of water quality. The gonad development of male fish of goldfish is harsh to environmental conditions, which is one of the keys to limit the artificial reproduction of goldfish. Orthogonal studies have shown that the salinity range suitable for the gonad development of money fish is 19-26‰, the temperature is 25-30 ℃, and the environment of micro-flow water can promote the gonad development of money fish.
Embodiment 2
[0031] Embodiment 2 parental synchronized sexual maturation technology
[0032] The gonad development of broodfish is asynchronous, and the male fish tends to mature earlier than the female fish, so the fertilization rate is low. In order to realize the synchronous development of the gonads of the broodfish of the chrysalis, the GnRH-A antagonist Goserelin (purchased from AstraZeneca Pharmaceuticals Co., Ltd., UK) was intrapleurally injected at 10-25 μg per kilogram of fish body weight one and a half months before artificial induction, which can significantly Delay the maturation of the testis of the money fish, and can ensure the synchronization of the gonad development of the parent fish of the money fish.
Embodiment 3
[0033] Example 3 Efficient artificial oxytocic agent and fully artificial oxytocic technology
[0034] Simply using oxytocin such as luteinizing hormone-releasing hormone analog LHRH and human chorionic gonadotropin HCG is not very effective in inducing labor in money fish, because the reaction time of money fish to oxytocin is long, and the effect of oxytocin is too long. It will degrade in the organism, which is also the reason why the hatching rate of other reported money fish is very low. We added a self-developed oxytocic slow-release agent (cholesterol: propylene glycol ester: triolein (mass ratio) = 40-50%: 20-30%: 20-30%) in the oxytocic agent, which is different from Other oxytocic slow-release methods, they make the slow-release oxytocic agent into cylindrical pills and granules, and implant them in the fish body. In this study, the developed slow-release agent was emulsified with the oxytocin LHRH and then injected into the pleural cavity. The oxytocin adjuvant do...
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