64 results about "Captive bred" patented technology

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The utility model discloses an artificial breeding device for light red ink head fish, which belongs to the technical field of aquatic product hatching and fry breeding and comprises a spawning induction pond, a hatching pond and a fry collecting pond. Cobblestones are laid at the bottom of the spawning induction pond, a spraying mechanism is fixedly connected to the top of the spawning induction pond, an ovulation pipe is communicated with the center of the spawning induction pond, the top of the ovulation pipe is lower than the top face of the spawning induction pond, and the bottom end of the ovulation pipe penetrates through the spawning induction pond to be communicated with the hatching pond; a filtering mechanism is detachably connected to the middle of the hatching pond, an overflow pipe is arranged in the filtering mechanism, and the bottom of the overflow pipe penetrates through the hatching pond to communicate with the outside; the side wall of the bottom of the hatching pond is communicated with a seedling outlet pipe, and the seedling outlet pipe is fixedly connected with a control valve and communicated with the seedling collecting pond. The device can effectively simulate the natural breeding environment of the light red ink head fish, accurately control various key parameters in the breeding process, and improve the reproductive performance of parent fish, the hatching rate of fish eggs and the survival rate of fish fries.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The invention relates to the technical field of gobio gobio artificial propagation, in particular to a gobio gobio artificial propagation method and device, and the device comprises supporting legs, a box body, a partition plate, a top cover, a cover plate, a handle, an auxiliary assembly and a moving assembly; a box body is fixedly installed on the multiple supporting legs, a partition plate is fixedly installed in the middle of the box body and divides the box body into a first cavity and a second cavity, two top covers are fixedly installed at the top of the box body and located on the two sides of the partition plate respectively, a cover plate is hinged to any top cover, and the cover plate is hinged to the bottom of the box body. A handle is fixedly installed on the cover plate, an auxiliary assembly is fixedly installed on one side of the long edge face of the box body, and a moving assembly is fixedly installed on one side of the short edge face of the box body. According to the gobio gobio spawn transfer device, irreversible damage caused in the transfer process of spawns during artificial propagation of gobio gobio is avoided, meanwhile, manual transfer is not needed, the transfer time is saved, the labor intensity is reduced, and the transfer efficiency is greatly improved.

The application discloses an artificial breeding and ovum collecting method of horseface fish, which can improve the quantity of obtained eggs, and the difference from the prior art is that the female fish without squeezed eggs is placed in a pool with water temperature of 18-20 DEG C for temporary breeding for 5-7 hours, taken out and placed in water with water temperature of 0-0.5 DEG C for 3-5 minutes, and then the female fish without squeezed eggs is subjected to second artificial egg squeezing and the egg grains are collected, the success rate of the second artificial egg squeezing can reach more than 95%, compared with the prior art of one-time egg squeezing, the quantity of obtained eggs can be improved by more than 20%, that is, the rate of inducing spawning is improved, and thus the quantity of fertilized eggs, the fertilization rate, the hatching rate and the total number of water flowers are improved.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The present application discloses an artificial propagation-hybridization combined breeding method for meat goats, which relates to the technical field of animal breeding. The specific steps include: selecting a goat breed with good meat performance as the maternal parent and selecting a goat breed with a large body as the paternal parent; culturing the maternal oocytes in vitro to mature oocytes containing the first polar body, using a temperature gradient screening device to screen out highly motile sperm, fertilizing the mature oocytes with the highly motile sperm in vitro, culturing them to a hybrid blastocyst morphology, transplanting them into the maternal body to develop until natural delivery, and obtaining a hybrid F1 generation; backcrossing the F1 generation with the maternal parent, and backcrossing the backcross offspring with the maternal parent multiple times until the breeding goal is met, and then cross-crossing and fixing them to obtain a new breed / line with excellent traits; improving the sperm screening efficiency by utilizing the screening device, ensuring the quality of the screened sperm, and thus significantly improving the fertilization efficiency and embryo survival rate.

The present application relates to a kind of artificial propagation method of Leucoglanis guichenoti.The artificial propagation efficiency of Leucoglanis guichenoti in prior art is low, and the rate of induced spawning, fertilization rate and hatching rate are not high.The propagation method of the present application comprises: using induced spawning agent (water temperature 13-19℃) to Leucoglanis guichenoti parent fish; the parent fish is put into spawning and hatching frame to spawn and fertilize naturally; after taking out the parent fish, keep the water level 5-15cm, and flow water hatching with 2-4 times / day exchange amount to obtain membrane fry; after another 24h culture, flat swimming fry is obtained.The induced spawning agent contains LRH-A2 and DOM, the dosage of female fish is LRH-A2 3-5ug / kg+DOM 5-10mg / kg, the dosage of male fish is LRH-A2 1-3ug / kg+DOM 2-5mg / kg, the effect time of induced spawning agent is 15-19h, the flow water hatching temperature is 17-19℃, and the duration is 42-45h.The method is simple in operation, low in cost, high in induced spawning rate, fertilization rate and hatching rate, can provide technical support for large-scale breeding of Leucoglanis guichenoti, promote the development of aquaculture industry, meet market demand, and has economic and social benefits.

The present application relates to the field of domestication of fish, and particularly relates to an artificial domestication method of Leptobotia rubipinnis. The method comprises the following steps: collecting and transporting wild parent fish, temporarily raising the wild parent fish, and domesticating the wild parent fish. The domestication process of the wild parent fish comprises the following steps: ① feeding biological bait, ② feeding biological bait and artificial compound feed, and ③ feeding artificial compound feed. The method has the advantages of simple operation process, and the use of the segmented temporary raising and domestication, gradual conversion from biological bait to artificial compound feed, and regulation of water flow rate during feeding, so that the death of the parent fish due to starvation is avoided, thereby improving the survival rate (more than 75%), the food conversion rate and the artificial domestication survival rate (≥68%) of the wild parent fish, and providing technical support and basic data accumulation for the artificial propagation, disease prevention and control and artificial propagation and release of Leptobotia rubipinnis.

The invention relates to a Chinese knot fish egg washing liquid, a Chinese knot fish breeding method and application thereof, and belongs to the technical field of aquaculture. According to the present invention, water is adopted as a solvent, and the water comprises 8-10 g / L of sodium chloride, 0.1-0.5 g / L of calcium chloride, 0.1-0.4 g / L of potassium chloride, and 10-15 g / L of glucose. By means of the breeding method, the fertilization rate, the hatching rate and the fry survival rate of the Chinese knot fish artificial breeding are greatly increased, the problems that in the Chinese knot fish artificial breeding process, female fish spawning is difficult, and the fertilization rate is low are solved, and large-scale production of the Chinese knot fish is achieved; the method is of great significance to species protection, field population resource recovery, breeding popularization and resource utilization of the Chinese knot fishes.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

This invention relates to the field of endangered aquatic biological resource protection and aquaculture technology, specifically to an integrated method for the harvesting, transportation, and domestication of the wavy-lipped barb, comprising the following steps: S1, behavioral adaptation-based nocturnal dive-fishing and decompression: during the wavy-lipped barb's nocturnal dormancy period, the target fish are gently induced into a pressure-regulating container; the fish are then safely transported to the water surface; S2, multi-stage live-preservation transportation: the fish treated in S1 are first transported via a live-water vessel for rehabilitation at sea, and then smoothly transferred via a live-water truck on land; S3, gradient-adaptive artificial domestication; S4, live germplasm resource preservation. This invention ensures that wild wavy-lipped barbs receive physiological protection at each transfer point, maximizing the maintenance of their internal environmental homeostasis, thereby increasing the overall survival rate to nearly 100% and providing high-quality, healthy parent fish for subsequent artificial breeding.

The invention discloses an out-of-season artificial propagation technical method for crucian carp. The technical method specifically comprises the key steps of water temperature and illumination control of an intelligent culture system, parent fish screening and concentrated intensified culture, parent fish ripening acceleration, artificial spawning induction, artificial insemination, incubation and the like. Aiming at seasonal limitation of natural breeding of the crucian carp, by scientifically regulating and controlling an illumination period and a water temperature environment and accurately simulating ecological conditions suitable for breeding of the crucian carp, it is ensured that parent fishes of the crucian carp can synchronously reach physiological states of periodic normal ovulation and spermiation in an unnatural spawning season from October to January of the next year; the activity and quality of germ cells can be obviously improved; meanwhile, an innovative breeding mode and an exclusive feeding scheme are combined, the industrial pain points that eggs of female crucian carp of ice cream are prone to aging and parent fishes only spawn once a year in a natural state are effectively overcome, and anti-season stable breeding is achieved.

The utility model relates to the technical field of crucian breeding, and discloses a temperature-control oxygen-increasing hatching device for artificial breeding of Chuzhou crucian carps, which comprises a hatching box, a hatching cavity, a circulating cavity and an equipment cavity, the hatching cavity, the circulating cavity and the equipment cavity are arranged in the hatching box, a first seedling raising layer and a second seedling raising layer are sequentially fixed in the hatching cavity from top to bottom, and a rotating hole is arranged on the inner wall between the hatching cavity and the circulating cavity. A stirring column is rotatably arranged in the rotating hole in a penetrating mode, a plurality of stirring fan blades are fixedly connected to the outer wall of the stirring column located in the hatching cavity in a sleeving mode, a plurality of water conveying holes are formed in the inner wall between the hatching cavity and the circulating cavity, and a plurality of blocking columns are slidably arranged in the water conveying holes in a penetrating mode. The crucian egg hatching device is reasonable in structure, convenient to use and high in working performance, can help to hatch crucian eggs, can purify water in the hatching device, can promote dispersion of the crucian eggs and fry, can regulate and control the temperature of culture water, further effectively improves the hatching rate of the crucian eggs, and can regulate the circulating speed of the water in the device.

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

The application provides a method for cultivating all-male hybrid yellow-head catfish by using double super-male population hybridization, comprising the following steps: S1, constructing super-male maintenance lines of yellow-head catfish and Pelteobagrus vishnui by using sex reversal technology and gender molecular markers; S2, performing artificial propagation on the maintenance lines to expand the super-male fish in batches; S3, using androgen antagonists to partially replace estrogen for the expanded super-male fish to cooperatively induce super-female fish; S4, applying specific nutrient enrichment culture to the super-female fish and super-male fish parents; and S5, performing positive and negative cross artificial propagation between the super-female fish and super-male fish of the two super-male populations to obtain a heterogamy super-male population, i.e., commercial fry of all-male hybrid yellow-head catfish. The technical scheme of the application can efficiently and in batches induce super-female fish, realize high-density culture of parent fish, and significantly improve the effect and efficiency of artificial propagation; the hybrid all-male yellow-head catfish has high uniformity, a growth speed of 20% and a male rate of 100%.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.

The utility model relates to aquatic products breeding equipment technical field provides a kind of goldfish artificial propagation post-rehabilitation maintenance device.The goldfish artificial propagation post-rehabilitation maintenance device includes box, drives component and partition component.The inside of box has cavity, and top is equipped with opening;Drive component is located in the inside of cavity, for driving fish;Partition component is located in box, for switching between first state and second state, in first state, partition component divides cavity into not intercommunicating feeding area and medicated bath area;In second state, feeding area and medicated bath area are communicated.The utility model solves the defect that repeated fishing operation leads to stress reaction intensification in prior art, realizes the rehabilitation nursing operation of exempting from transfer processing, improves goldfish recovery quality and efficiency.

The utility model provides a horse artificial breeding seminal fluid storage device which comprises a liquid nitrogen box and a thin storage tube, one side of the liquid nitrogen box is connected with an annular frame in a rotating mode, the annular frame and a part section on the annular frame enter the liquid nitrogen box, and the combination position of the annular frame and the liquid nitrogen box is matched in a sealing mode. The upper end face of the annular frame is provided with a containing groove which is distributed in a winding mode and used for containing and storing thin pipes. According to the device, the annular frame passing through the inner cavity of the liquid nitrogen box is arranged, the annular frame and the liquid nitrogen box are rotationally connected, the contact position of the annular frame and the liquid nitrogen box is in a sealed sliding state, then the containing groove used for containing the thin pipe is formed in the top of the annular frame, and the thin pipe located in the inner cavity of the liquid nitrogen box can be transferred to the outside when the annular frame rotates; the arrangement can prevent liquid nitrogen from radiating outwards at low temperature, and compared with the prior art, the device has the advantage of isolating low temperature when the thin tube is taken and placed.

The invention discloses a method for conveniently and noninvasively recovering parents from a net cage after large-scale artificial breeding of ricefield eels, which comprises the following steps of: deepening pond water in a breeding soil pond to force the ricefield eels to get out of soil, and putting a net tool soaked in the pond water into the center of the net cage in the breeding soil pond, a small gauze package containing earthworms is placed in the center of the upper portion of the top of each net, and the finless eels are trapped into the nets; starting to harvest ricefield eel parents in the morning of the next day, lifting the netting gear from each net cage one by one, enabling one corner of the bottom of the netting gear to be the lowest and to be aligned with the opening of the plastic box for recovering the parents, enabling the trapped ricefield eel parents to slide to the corner, pushing aside the opening near the ricefield eel by hands, enabling the ricefield eel to slide into the plastic box from the opening, and counting; according to the method, the nylon net which suspends in water and is provided with the earthworm bait is used for trapping the ricefield eels into the net, so that the purpose that parents are conveniently and efficiently recaptured and bred and are not injured is achieved, and the parent recapturing rate of the method can reach 95% or above.

The present invention provides a method for artificial propagation of Acipenser dabryanus, which includes selecting broodstock, pairing broodstock, culturing broodstock, artificial induced spawning, artificial insemination, hatching of fertilized eggs and culturing of juvenile fish. The present invention stimulates the gonadal development of broodstock by precisely controlling the water temperature during broodstock cultivation, selects broodstock with relatively distant genetic relationships for pairing through the identification of the genetic relationships of broodstock to overcome the problem of population degradation caused by inbreeding, and uses artificial insemination, hatching of fertilized eggs and juvenile fish cultivation techniques to solve the problem of difficult artificial propagation of existing Acipenser dabryanus, so as to stably and continuously supply high-quality Acipenser dabryanus seeds and meet the perennial effective demand for artificial enhancement and release of Acipenser dabryanus.

The application discloses a large water body eutrophication rapid treatment method and a special system thereof, and comprises a harbor type net enclosure captive breeding salvage unit, a water level self-adaptive cutting pump suction unit, a flume self-flow transportation unit and a solid-liquid separation resource unit. The method absorbs pollutants through net enclosure captive breeding purification organisms, and the whole net is dragged to a shore-based still water harbor by a tugboat during salvage. The organisms enter a cutting bin through a water level self-adaptive gate and the net is recovered synchronously. After the organisms are cut and broken into slurry, the slurry is lifted to an overhead flume by a cutter suction pump, and is self-flowed to a solid-liquid separation bin by gravity. Finally, the slurry is dewatered through filter pipe seepage, anaerobic fermentation and enhanced pressure filtration, so as to realize resource utilization of biogas, organic fertilizer and irrigation water. The application has very strong treatment capacity, is ecological friendly, has very low energy consumption and cost, and can rapidly improve the water quality of a large water body from poor V class to I-II class.

The present application relates to the technical field of aquaculture, and particularly relates to an artificial breeding method of Cottus hangi in Xingkai Lake. The present application uses LRH-A2 and HCG combination oxytocin to reasonably inject female parent fish and male parent fish, especially to twice injection of female parent fish, and strictly controls injection dose of each injection and reasonable control time interval, which not only can efficiently induce labor, but also can reach 95% of the female parent fish induction efficiency, and can obtain good ovum and semen, so that the ovum can be better fertilized, the quality of the fertilized ovum is improved, the hatching rate is improved, the stress of the parent fish is reduced, and the death of the parent fish is reduced. The present application improves the physical fitness of the parent fish through reasonable parent fish cultivation, and combines reasonable artificial induction operation and post-production nursing measures, so that the post-production death of the parent fish is reduced from multiple aspects, and the post-production survival rate of the parent fish is significantly improved, so that the repeated cultivation cost of the parent fish is reduced.

The present invention relates to the technical field of Phoenix snail breeding, and in particular to an artificial breeding method for Phoenix snails. The method comprises the following steps: randomly selecting 60 red Phoenix snails with good vitality, wiping off excess moisture on the surfaces of the red Phoenix snails, measuring morphological parameters of the red Phoenix snails using a vernier caliper, weighing the body mass Y1 using an electronic balance, then dissecting the sample, weighing the mass Y2 of the soft body, performing anatomical observation, and distinguishing and recording the sexes. The method develops artificial breeding technology for the red Phoenix snails by observing the reproductive biological characteristics of the red Phoenix snails, providing a scientific basis for the artificial breeding of the species, and having important significance for promoting the sustainable utilization and protection of the red Phoenix snails, thereby promoting the development and promotion of artificial breeding technology for the red Phoenix snails, being conducive to meeting market demand, increasing the income of the breeding industry, reducing wild fishing, and promoting the sustainable utilization and protection of its ecological resources.

The embodiment of the invention discloses an artificial breeding fish releasing site selection method, device and equipment and a storage medium, and relates to the technical field of water conservancy project ecological protection, and the method comprises the steps: determining the optimal flow velocity interval for the survival and inhabitation of target fishes through sampling the relationship between the distribution number of the target fishes and the flow velocity of the distribution site; obtaining an upstream induction flow velocity of the target fish by using a fish swimming ability test; establishing a hydraulic model based on the operation condition of the release area reservoir area, and calculating the flow velocity spatial distribution of the release area reservoir area under the target condition through the hydraulic model; and determining a target releasing site according to the flow velocity spatial distribution, the optimal flow velocity interval for survival and inhabitation of the target fish and the induction flow velocity for upstream tracking of the target fish. The technical problems that in the prior art, an empirical fish releasing method is adopted, so that the survival rate of fishes is not controlled, and the proliferation effect is poor can be solved.

The present application relates to the field of artificial breeding of Pygopristis oligolepis, and in particular to a Pygopristis oligolepis egg collecting device, comprising a parent fish pool, a collecting pool, a water pump and an egg collecting tube; an inner cavity of the parent fish pool comprises an ovipositing chamber and a receiving chamber arranged in an up-down manner, a simulated cobblestone plate is arranged between the ovipositing chamber and the receiving chamber, the simulated cobblestone plate is provided with through holes, the receiving chamber is connected with the collecting pool through a collecting tube and the water pump, and the collecting pool is connected with the ovipositing chamber through a water return tube and the water pump; one end of the egg collecting tube extends into the ovipositing chamber in the parent fish pool, the one end of the egg collecting tube is provided with a vertical section, a surface of the vertical section is provided with two or more egg collecting openings along an axis at intervals, a funnel-shaped egg collecting plate is arranged below each of the egg collecting openings, and the other end of the egg collecting tube is connected with the collecting pool through the water pump. The Pygopristis oligolepis egg collecting device provided by the present application can effectively prevent the parent fish from eating the eggs after spawning, and is conducive to the development of large-scale breeding.

The invention discloses an indoor algal-shellfish symbiosis artificial culture system and method. The system comprises a culture box with a box cover, an adhesion matrix device and a water environment control system, a partition plate is vertically arranged in the incubator, so that a water flow channel is formed between the partition plate and the inner wall of the incubator, and two functional areas, namely a first area and a second area, are further formed on the two sides of the partition plate; the substrate attaching device is arranged in the second area; the water environment control system comprises a flow speed regulation and control unit, an illumination unit, a heating unit, an oxygen dissolving unit and a control unit, and the flow speed regulation and control unit, the illumination unit, the heating unit and the oxygen dissolving unit are all distributed in the first area and controlled by the control unit. The method comprises the following steps: adding shellfish onto an adhesion substrate device; adding algae into the second area; the water environment control system is used for regulating and controlling the water body flow velocity, illumination intensity, water body temperature and water body dissolved oxygen. The method is applied to indoor artificial propagation of the limnoperna fortunei, and the artificial breeding survival rate of the limnoperna fortunei can be increased.

The invention relates to the technical field of aquaculture, in particular to an indoor artificial propagation method for trachinotus ovatus. Comprising the steps of parent selection; transferring and transporting for multiple times, anesthetizing by using anesthetics with different concentrations during the period, and transferring to an indoor circulating water culture system for culture after fresh water bath and disinfection; adding purified seawater and adjusting the water temperature in the early stage of cultivation; starting an indoor recirculating aquaculture system; after the parents enter the indoor recirculating aquaculture system and feed is stopped for 3 days, chilled fresh fishes are fed firstly, compound feed with the chilled fresh fishes and the parents in the initial mass ratio of 3: 1 is fed on the fourth day, and the compound feed is completely fed on the fifteenth day; krill meal and krill oil are added into the parent compound feed in the early stage and the later stage of cultivation respectively, and chilled fresh fish is fed once every three days; after the gonad of the parent is mature, injecting oxytocic to the female fish for three times; the injection time is synchronous with the third injection time of the female fish; after injection, the parent fishes begin to spawn, roes are collected and hatched, and the spawning rate, the fertilization rate and the hatching rate are greatly increased.