54 results about "Captive bred" patented technology

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The invention relates to the technical field of gobio gobio artificial propagation, in particular to a gobio gobio artificial propagation method and device, and the device comprises supporting legs, a box body, a partition plate, a top cover, a cover plate, a handle, an auxiliary assembly and a moving assembly; a box body is fixedly installed on the multiple supporting legs, a partition plate is fixedly installed in the middle of the box body and divides the box body into a first cavity and a second cavity, two top covers are fixedly installed at the top of the box body and located on the two sides of the partition plate respectively, a cover plate is hinged to any top cover, and the cover plate is hinged to the bottom of the box body. A handle is fixedly installed on the cover plate, an auxiliary assembly is fixedly installed on one side of the long edge face of the box body, and a moving assembly is fixedly installed on one side of the short edge face of the box body. According to the gobio gobio spawn transfer device, irreversible damage caused in the transfer process of spawns during artificial propagation of gobio gobio is avoided, meanwhile, manual transfer is not needed, the transfer time is saved, the labor intensity is reduced, and the transfer efficiency is greatly improved.

The application discloses an artificial breeding and ovum collecting method of horseface fish, which can improve the quantity of obtained eggs, and the difference from the prior art is that the female fish without squeezed eggs is placed in a pool with water temperature of 18-20 DEG C for temporary breeding for 5-7 hours, taken out and placed in water with water temperature of 0-0.5 DEG C for 3-5 minutes, and then the female fish without squeezed eggs is subjected to second artificial egg squeezing and the egg grains are collected, the success rate of the second artificial egg squeezing can reach more than 95%, compared with the prior art of one-time egg squeezing, the quantity of obtained eggs can be improved by more than 20%, that is, the rate of inducing spawning is improved, and thus the quantity of fertilized eggs, the fertilization rate, the hatching rate and the total number of water flowers are improved.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The present application relates to a kind of artificial propagation method of Leucoglanis guichenoti.The artificial propagation efficiency of Leucoglanis guichenoti in prior art is low, and the rate of induced spawning, fertilization rate and hatching rate are not high.The propagation method of the present application comprises: using induced spawning agent (water temperature 13-19℃) to Leucoglanis guichenoti parent fish; the parent fish is put into spawning and hatching frame to spawn and fertilize naturally; after taking out the parent fish, keep the water level 5-15cm, and flow water hatching with 2-4 times / day exchange amount to obtain membrane fry; after another 24h culture, flat swimming fry is obtained.The induced spawning agent contains LRH-A2 and DOM, the dosage of female fish is LRH-A2 3-5ug / kg+DOM 5-10mg / kg, the dosage of male fish is LRH-A2 1-3ug / kg+DOM 2-5mg / kg, the effect time of induced spawning agent is 15-19h, the flow water hatching temperature is 17-19℃, and the duration is 42-45h.The method is simple in operation, low in cost, high in induced spawning rate, fertilization rate and hatching rate, can provide technical support for large-scale breeding of Leucoglanis guichenoti, promote the development of aquaculture industry, meet market demand, and has economic and social benefits.

The present application relates to the field of domestication of fish, and particularly relates to an artificial domestication method of Leptobotia rubipinnis. The method comprises the following steps: collecting and transporting wild parent fish, temporarily raising the wild parent fish, and domesticating the wild parent fish. The domestication process of the wild parent fish comprises the following steps: ① feeding biological bait, ② feeding biological bait and artificial compound feed, and ③ feeding artificial compound feed. The method has the advantages of simple operation process, and the use of the segmented temporary raising and domestication, gradual conversion from biological bait to artificial compound feed, and regulation of water flow rate during feeding, so that the death of the parent fish due to starvation is avoided, thereby improving the survival rate (more than 75%), the food conversion rate and the artificial domestication survival rate (≥68%) of the wild parent fish, and providing technical support and basic data accumulation for the artificial propagation, disease prevention and control and artificial propagation and release of Leptobotia rubipinnis.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

This invention relates to the field of endangered aquatic biological resource protection and aquaculture technology, specifically to an integrated method for the harvesting, transportation, and domestication of the wavy-lipped barb, comprising the following steps: S1, behavioral adaptation-based nocturnal dive-fishing and decompression: during the wavy-lipped barb's nocturnal dormancy period, the target fish are gently induced into a pressure-regulating container; the fish are then safely transported to the water surface; S2, multi-stage live-preservation transportation: the fish treated in S1 are first transported via a live-water vessel for rehabilitation at sea, and then smoothly transferred via a live-water truck on land; S3, gradient-adaptive artificial domestication; S4, live germplasm resource preservation. This invention ensures that wild wavy-lipped barbs receive physiological protection at each transfer point, maximizing the maintenance of their internal environmental homeostasis, thereby increasing the overall survival rate to nearly 100% and providing high-quality, healthy parent fish for subsequent artificial breeding.

The invention discloses an out-of-season artificial propagation technical method for crucian carp. The technical method specifically comprises the key steps of water temperature and illumination control of an intelligent culture system, parent fish screening and concentrated intensified culture, parent fish ripening acceleration, artificial spawning induction, artificial insemination, incubation and the like. Aiming at seasonal limitation of natural breeding of the crucian carp, by scientifically regulating and controlling an illumination period and a water temperature environment and accurately simulating ecological conditions suitable for breeding of the crucian carp, it is ensured that parent fishes of the crucian carp can synchronously reach physiological states of periodic normal ovulation and spermiation in an unnatural spawning season from October to January of the next year; the activity and quality of germ cells can be obviously improved; meanwhile, an innovative breeding mode and an exclusive feeding scheme are combined, the industrial pain points that eggs of female crucian carp of ice cream are prone to aging and parent fishes only spawn once a year in a natural state are effectively overcome, and anti-season stable breeding is achieved.

The utility model relates to the technical field of crucian breeding, and discloses a temperature-control oxygen-increasing hatching device for artificial breeding of Chuzhou crucian carps, which comprises a hatching box, a hatching cavity, a circulating cavity and an equipment cavity, the hatching cavity, the circulating cavity and the equipment cavity are arranged in the hatching box, a first seedling raising layer and a second seedling raising layer are sequentially fixed in the hatching cavity from top to bottom, and a rotating hole is arranged on the inner wall between the hatching cavity and the circulating cavity. A stirring column is rotatably arranged in the rotating hole in a penetrating mode, a plurality of stirring fan blades are fixedly connected to the outer wall of the stirring column located in the hatching cavity in a sleeving mode, a plurality of water conveying holes are formed in the inner wall between the hatching cavity and the circulating cavity, and a plurality of blocking columns are slidably arranged in the water conveying holes in a penetrating mode. The crucian egg hatching device is reasonable in structure, convenient to use and high in working performance, can help to hatch crucian eggs, can purify water in the hatching device, can promote dispersion of the crucian eggs and fry, can regulate and control the temperature of culture water, further effectively improves the hatching rate of the crucian eggs, and can regulate the circulating speed of the water in the device.

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

The application provides a method for cultivating all-male hybrid yellow-head catfish by using double super-male population hybridization, comprising the following steps: S1, constructing super-male maintenance lines of yellow-head catfish and Pelteobagrus vishnui by using sex reversal technology and gender molecular markers; S2, performing artificial propagation on the maintenance lines to expand the super-male fish in batches; S3, using androgen antagonists to partially replace estrogen for the expanded super-male fish to cooperatively induce super-female fish; S4, applying specific nutrient enrichment culture to the super-female fish and super-male fish parents; and S5, performing positive and negative cross artificial propagation between the super-female fish and super-male fish of the two super-male populations to obtain a heterogamy super-male population, i.e., commercial fry of all-male hybrid yellow-head catfish. The technical scheme of the application can efficiently and in batches induce super-female fish, realize high-density culture of parent fish, and significantly improve the effect and efficiency of artificial propagation; the hybrid all-male yellow-head catfish has high uniformity, a growth speed of 20% and a male rate of 100%.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.

The utility model relates to aquatic products breeding equipment technical field provides a kind of goldfish artificial propagation post-rehabilitation maintenance device.The goldfish artificial propagation post-rehabilitation maintenance device includes box, drives component and partition component.The inside of box has cavity, and top is equipped with opening;Drive component is located in the inside of cavity, for driving fish;Partition component is located in box, for switching between first state and second state, in first state, partition component divides cavity into not intercommunicating feeding area and medicated bath area;In second state, feeding area and medicated bath area are communicated.The utility model solves the defect that repeated fishing operation leads to stress reaction intensification in prior art, realizes the rehabilitation nursing operation of exempting from transfer processing, improves goldfish recovery quality and efficiency.

The application discloses a large water body eutrophication rapid treatment method and a special system thereof, and comprises a harbor type net enclosure captive breeding salvage unit, a water level self-adaptive cutting pump suction unit, a flume self-flow transportation unit and a solid-liquid separation resource unit. The method absorbs pollutants through net enclosure captive breeding purification organisms, and the whole net is dragged to a shore-based still water harbor by a tugboat during salvage. The organisms enter a cutting bin through a water level self-adaptive gate and the net is recovered synchronously. After the organisms are cut and broken into slurry, the slurry is lifted to an overhead flume by a cutter suction pump, and is self-flowed to a solid-liquid separation bin by gravity. Finally, the slurry is dewatered through filter pipe seepage, anaerobic fermentation and enhanced pressure filtration, so as to realize resource utilization of biogas, organic fertilizer and irrigation water. The application has very strong treatment capacity, is ecological friendly, has very low energy consumption and cost, and can rapidly improve the water quality of a large water body from poor V class to I-II class.

The present application relates to the technical field of aquaculture, and particularly relates to an artificial breeding method of Cottus hangi in Xingkai Lake. The present application uses LRH-A2 and HCG combination oxytocin to reasonably inject female parent fish and male parent fish, especially to twice injection of female parent fish, and strictly controls injection dose of each injection and reasonable control time interval, which not only can efficiently induce labor, but also can reach 95% of the female parent fish induction efficiency, and can obtain good ovum and semen, so that the ovum can be better fertilized, the quality of the fertilized ovum is improved, the hatching rate is improved, the stress of the parent fish is reduced, and the death of the parent fish is reduced. The present application improves the physical fitness of the parent fish through reasonable parent fish cultivation, and combines reasonable artificial induction operation and post-production nursing measures, so that the post-production death of the parent fish is reduced from multiple aspects, and the post-production survival rate of the parent fish is significantly improved, so that the repeated cultivation cost of the parent fish is reduced.

The embodiment of the invention discloses an artificial breeding fish releasing site selection method, device and equipment and a storage medium, and relates to the technical field of water conservancy project ecological protection, and the method comprises the steps: determining the optimal flow velocity interval for the survival and inhabitation of target fishes through sampling the relationship between the distribution number of the target fishes and the flow velocity of the distribution site; obtaining an upstream induction flow velocity of the target fish by using a fish swimming ability test; establishing a hydraulic model based on the operation condition of the release area reservoir area, and calculating the flow velocity spatial distribution of the release area reservoir area under the target condition through the hydraulic model; and determining a target releasing site according to the flow velocity spatial distribution, the optimal flow velocity interval for survival and inhabitation of the target fish and the induction flow velocity for upstream tracking of the target fish. The technical problems that in the prior art, an empirical fish releasing method is adopted, so that the survival rate of fishes is not controlled, and the proliferation effect is poor can be solved.

The present application relates to the field of artificial breeding of Pygopristis oligolepis, and in particular to a Pygopristis oligolepis egg collecting device, comprising a parent fish pool, a collecting pool, a water pump and an egg collecting tube; an inner cavity of the parent fish pool comprises an ovipositing chamber and a receiving chamber arranged in an up-down manner, a simulated cobblestone plate is arranged between the ovipositing chamber and the receiving chamber, the simulated cobblestone plate is provided with through holes, the receiving chamber is connected with the collecting pool through a collecting tube and the water pump, and the collecting pool is connected with the ovipositing chamber through a water return tube and the water pump; one end of the egg collecting tube extends into the ovipositing chamber in the parent fish pool, the one end of the egg collecting tube is provided with a vertical section, a surface of the vertical section is provided with two or more egg collecting openings along an axis at intervals, a funnel-shaped egg collecting plate is arranged below each of the egg collecting openings, and the other end of the egg collecting tube is connected with the collecting pool through the water pump. The Pygopristis oligolepis egg collecting device provided by the present application can effectively prevent the parent fish from eating the eggs after spawning, and is conducive to the development of large-scale breeding.

The invention relates to the technical field of aquaculture, in particular to an indoor artificial propagation method for trachinotus ovatus. Comprising the steps of parent selection; transferring and transporting for multiple times, anesthetizing by using anesthetics with different concentrations during the period, and transferring to an indoor circulating water culture system for culture after fresh water bath and disinfection; adding purified seawater and adjusting the water temperature in the early stage of cultivation; starting an indoor recirculating aquaculture system; after the parents enter the indoor recirculating aquaculture system and feed is stopped for 3 days, chilled fresh fishes are fed firstly, compound feed with the chilled fresh fishes and the parents in the initial mass ratio of 3: 1 is fed on the fourth day, and the compound feed is completely fed on the fifteenth day; krill meal and krill oil are added into the parent compound feed in the early stage and the later stage of cultivation respectively, and chilled fresh fish is fed once every three days; after the gonad of the parent is mature, injecting oxytocic to the female fish for three times; the injection time is synchronous with the third injection time of the female fish; after injection, the parent fishes begin to spawn, roes are collected and hatched, and the spawning rate, the fertilization rate and the hatching rate are greatly increased.

The application discloses an indoor algae-be symbiotic artificial culture system and method, and belongs to the field of artificial culture of algae-be symbiotic system. The system comprises a culture box with a box cover, an attachment substrate device and a water environment control system. A partition plate is vertically arranged in the culture box to form a water flow channel between the partition plate and the inner wall of the culture box, and two functional areas, i.e. a first area and a second area, are formed on the two sides of the partition plate. The attachment substrate device is arranged in the second area. The water environment control system comprises a flow rate regulating unit, a light unit, a heating unit, a dissolved oxygen unit and a control unit. The flow rate regulating unit, the light unit, the heating unit and the dissolved oxygen unit are arranged in the first area and are controlled by the control unit. The method comprises the following steps: adding shellfish to the attachment substrate device; adding algae to the second area; and regulating the water flow rate, the light intensity, the water temperature and the water dissolved oxygen by the water environment control system. The application is applied to the indoor artificial breeding of freshwater zebra mussels and can improve the survival rate of the artificial breeding of the freshwater zebra mussels.

This utility model discloses a single-box circulating algae-shell symbiotic cultivation device, including a cultivation box with a lid, a water environment control system, and an attachment substrate device. A partition plate is erected inside the cultivation box to form a water flow channel between the partition plate and the inner wall of the cultivation box, and two functional areas are formed on both sides of the partition plate: a first area and a second area. The water environment control system includes a flow rate control unit, which is located in the first area; the attachment substrate device is located in the second area. The flow rate control unit controls the water contained in the cultivation box to circulate along the water flow channel at a preset flow rate. This utility model has a simple structure. When applied to the indoor artificial propagation of freshwater shellfish, it can improve the ecological balance between freshwater shellfish and algae, and promote the survival rate of artificially cultivated freshwater shellfish.

This invention discloses a biomimetic nest box for captive red pandas, belonging to the technical field of wildlife captive breeding facilities. The nest box includes a box body, a partition assembly, a keeper operation door assembly, an animal entrance / exit assembly, and a temperature control assembly. The partition assembly divides the inner cavity of the box body into upper and lower independent sections. The sides of the box body have two keeper operation doors with observation windows, and the front has an animal entrance / exit with a circular movable door connected to the box body via a hinge. The interior of the box body contains a thermometer and an ice pack container. This invention simulates the environment of a natural tree hole nest through biomimetic design, utilizes an independent dual-channel design to separate keeper operation from animal entry / exit, and combines passive temperature control to effectively reduce human interference and maintain the temperature inside the nest box within a suitable range. It aims to solve the problems of existing nest boxes being simple in structure, lacking biomimetic design, lacking temperature control methods, and inconvenient to operate, significantly improving the survival rate of captive red panda cubs during the breeding season.

The application discloses an artificial breeding method of oriental blackspot fish, comprising the following steps: S1, parent fish selection; S2, parturition injection; S3, insemination; S4, fertilized egg hatching; and S5, fry cultivation. The artificial breeding method of the oriental blackspot fish provided by the application aims at the technical problems of the oriental blackspot fish, such as inconsistent response of parent fish to parturition, low insemination efficiency caused by large amount of sperm, and harsh hatching conditions of drifting eggs, and the like, and the method comprises the following steps: establishing a differentiated parturition scheme for female and male, a special sperm diluent formula, a linkage quantitative insemination system, an accurate constant-temperature high-dissolved-oxygen hatching process, forming a complete artificial breeding technical system covering parent fish selection to fry cultivation, and realizing the standardization and repeatable operation of each key link of the artificial breeding of the oriental blackspot fish, thereby providing systematic technical support for the large-scale fry production of the species.

The invention discloses a method for producing all-male largemouth micropterus salmoides based on soaking induction of sex reversal of largemouth micropterus salmoides, and particularly relates to the field of artificial propagation of all-male largemouth micropterus salmoides, the method comprises the following steps: S01, taking fairy shrimps as initial feed when largemouth micropterus salmoides fries obtained by incubation swim flatly; s02, transferring the largemouth micropterus salmoides fry into a water body in which a sexual reversal induction reagent is dissolved, keeping feeding three times every day during the period, and soaking and breeding the fry for 60 days; and S03, after soaking and culturing for 60 days, transferring the largemouth bass fry into a conventional culture water body for culturing, and after sexual maturity of the largemouth bass fry, obtaining a male population capable of generating seminal fluid, namely the all-male largemouth bass. According to the method, largemouth bass fries are soaked in a sex reversal induction reagent with a specific concentration, sex reversal fishes with a high survival rate and a low malformation rate can be directly obtained, and then large-scale cultivation of all-male largemouth bass is achieved.

The invention provides an artificial propagation method for sculpin versicolor, and belongs to the technical field of fish culture. The artificial spawning induction method comprises the following steps that spawning induction hormone is injected into female parents for breeding parent fishes for three times for artificial spawning induction, the ratio of the female parent fishes to the male parent fishes is adjusted to be (2-3): 1, and fertilized eggs are obtained. The fertilized eggs are placed in a hatching basket to be hatched, a sunshade net is built above the fertilized eggs to shield light, the water temperature during hatching is 10-15 DEG C, dissolved oxygen is 5-10 mg / L, and larva fishes are obtained. According to the method, spawning induction hormone is injected in a three-needle injection mode, the injection dosage and the interval time of each needle are strictly controlled, eggs and seminal fluid which are well developed can be obtained, meanwhile, the hatching conditions of the fertilized eggs are controlled, the spawning induction rate of sculpin versicolor reaches 84.03%, the fertilization rate reaches 93.5% or above, and the hatching rate reaches 87% or above; the small-scale artificial propagation of the sculpin is realized, and the method has important significance for protecting the endangered sculpin which is an endangered resource.

The invention relates to the technical field of breeding of endangered wild protected fishes, in particular to a full-artificial breeding device and method for promoting natural spawning of cheilocheilus undulatus. The side wall of the spawning pond is communicated with a water inlet pipe and a drainage pipe; a plurality of flow guide ditches are formed in the bottom of the spawning pond, and an egg collecting assembly used for collecting roes of the lissocheilus undulatus is arranged on the drainage pipe; a wave making assembly for simulating tidal water flow to activate the breeding behavior of the cheilocheilus undulatus is arranged in the water pool; the wave making assembly comprises water retaining blocks symmetrically and fixedly connected to the side wall of the spawning pond and a support fixedly connected to the inner side wall of the spawning pond, a driving part is arranged on the support, and the controller is used for controlling an output shaft of the driving part to rotate; fan blades are coaxially and fixedly connected to an output shaft of the driving part; the support is provided with a swing assembly used for driving the driving piece to swing up and down. By simulating a natural reef area tidal water flow environment, parent fishes are induced to naturally lay eggs without intervention of exogenous hormones, so that the breeding success rate is increased.

The invention discloses a karst cave matrix bionic fish nest for breeding sinocyclocheilus multispot. The karst cave matrix bionic fish nest comprises a fry hatching area. The inside of the fry hatching area is divided into a karst cave sediment area, a clay planting area and a gentle slope transition area; a karst cave sediment layer is laid in the karst cave sediment area, and bionic rock blocks are laid on the surface of the karst cave sediment layer; the bionic rock block is prepared from natural karst cave limestone in the karst region, and at least one of a vertical drill hole, a transverse drill hole and a texture structure is formed in the bionic rock block; submerged plants are planted in the clay planting area, and the gentle slope transition area is connected with the karst cave sediment area and the clay planting area; aerated karst cave water or simulated karst cave water is injected into the fry hatching area, and the water injection depth is 20-80 cm. According to the method, the technical problems of strong parent fish stress reaction, low gonad development synchronization rate, insufficient spawning willingness, poor roe adhesion stability, low hatching rate and the like in artificial propagation of the sinocyclocheilus multimaculatus are solved.