10 results about "Artificial fertilization" patented technology

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

The application provides a mature rubber tree fertilization method based on nutrient circulation, which comprises the following steps: step one, establishing a relational expression Y i = litter decomposition + rainfall input + tree leaching + artificial fertilization; Y o = tree absorption + surface runoff; step two, using the soil nutrient balance principle, i.e. nutrient input Y i = nutrient output Y o , the above formula is brought into, and litter decomposition + rainfall input + tree leaching + artificial fertilization = tree absorption + surface runoff is obtained; step three, through nutrient calculation and data analysis, a function relationship between the recommended amount of fertilization Y f of the mature rubber tree and the tree age X is fitted, i.e. Y f =aX 2 +bX+c; the application solves the contradiction that the fertilization management does not meet the production demand caused by the problems such as single rubber tree fertilization formula, old diagnosis technology and difficult-to-determine test analysis index, and has good yield-increasing effect and production practicability.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The application provides a dairy cow mating auxiliary device, which comprises two symmetrically distributed side frames, a front frame capable of clamping a neck of a cow is fixed between one end of the two side frames, the other end of one of the side frames is provided with a clamping ring with a circular ring structure, the ring wall of the clamping ring is a hollow structure, the inner ring wall of the clamping ring is made of elastic rubber material, and the ring wall of the clamping ring is connected with a gas pressure adjusting device capable of adjusting the gas pressure in the ring wall. In the artificial insemination process, the labor intensity of the staff can be reduced without artificial hand pulling of the cow tail, and the stability of the cow tail clamped by the inner ring wall is relatively high, so that the cow tail cannot break away from the clamping ring even if the cow resists and swings the tail.

The invention relates to a fertilizing device for agricultural cooperative planting, the fertilizing device comprises an unmanned aerial vehicle seat, a support member connected below the unmanned aerial vehicle seat and a wing member connected above the unmanned aerial vehicle seat, the unmanned aerial vehicle seat is connected with a fertilizing member, and the fertilizing member comprises a water spraying support connected to the unmanned aerial vehicle seat and a water spraying head connected to the water spraying support; the unmanned aerial vehicle seat is connected with a liquid fertilizer box. The technical scheme has the beneficial technical effects that the wing parts can drive the unmanned aerial vehicle base to flexibly move, a large-area planting area can be quickly covered, the problems that the cooperative planting area is wide, manual fertilization is time-consuming and labor-consuming, and the efficiency is low are effectively solved, the human input cost is reduced, and the fertilization operation period is shortened; the high-efficiency operation requirement of large-scale planting of a cooperative is met; the fertilizing part is composed of a water spraying support and a water spraying head and is matched with the liquid fertilizer box to continuously supply liquid fertilizer, and the liquid fertilizer can be evenly sprayed to the surfaces and root areas of crops.

The utility model relates to a frozen semen unfreezing water temperature accurate control device, and belongs to the technical field of artificial fertilization for livestock. The unfreezing water tank is embedded in the top of the shell, the electric heating plate is installed at the bottom in the unfreezing water tank, the motor is installed at the bottom in the unfreezing water tank, the stirring impeller is installed at the bottom in the unfreezing water tank, the motor is in transmission connection with the stirring impeller, the temperature sensing probe is installed in the unfreezing water tank, and the semen freezing pipe limiting device is installed in the unfreezing water tank. The plc is mounted in the electric appliance mounting cavity, and the control panel is embedded in the side wall of the shell, so that the change of the water temperature can be intuitively known; the temperature sensing probe, the control panel, the electric heating plate and the motor are connected with a plc (programmable logic controller); the top cover covers the top of the thawing water tank; when the temperature is too low, the water body is heated through the electric heating plate, and the water body temperature is kept within a proper range; thawing water is slowly stirred through the motor and the stirring impeller, water temperature uniformity is guaranteed, and the problem of large water temperature difference at different positions is solved. The semen thawing effect is improved, and the working efficiency is improved.

The invention discloses a method and a system for regulating and controlling synchronous reproduction of large yellow croakers by utilizing reproduction period barks. The method comprises the following steps: selecting healthy larimichthys crocea parent fishes from the end of the IV stage to the end of the V stage, pairing one female larimichthys crocea parent fishes and performing spawning induction; within 20-40 hours after spawning induction, acoustic stimulation signals obtained on the basis of mutals in the breeding period of the parent fishes are played to the parent fishes, and the signal characteristics include the main peak frequency of 163-673Hz, the pulse width of about 2ms, the interval of about 67ms and the sound pressure level of 116.6-123.4 dB re < 1 > mu Pa; after spawning is monitored, semen is immediately obtained for artificial fertilization. The system comprises a storage playing module and an underwater sound radiation device. According to the method, synchronous spawning and spermiation of the parent fishes under one-to-one pairing are effectively promoted, and the spawning rate and the fertilization rate are remarkably increased.

This invention relates to a method for regulating the late autumn breeding of mandarin fish, belonging to the field of artificial fish breeding in aquaculture technology. The operation of this invention is as follows: (1) Inducing spawning in spring by injecting a high dose of a single oxytocin into the mandarin fish parent stock; (2) Under the action of the high dose of the single oxytocin, partial spawning is completed, achieving an average spawning volume per female fish of 20%-30% of the theoretical spawning volume in spring; (3) After spawning until the end of June, controlled feeding management is carried out to reduce the amount of feed, so that the fish are in a nutritional state of maintenance or slow growth; (4) In July and August, nutritional enhancement cultivation is carried out, and sufficient feeding is restored for a period of time before formal spawning to promote the rapid secondary development of the gonads of the parent mandarin fish; oxytocin is injected at a conventional effective dose to induce artificial insemination of the parent mandarin fish, resulting in concentrated spawning, achieving an average spawning volume per female fish of 80%-90% of the spawning volume in autumn. This invention can achieve the dual use of mandarin fish parent stock in one year through conventional hormone injection and feed management.

The invention relates to a culture regulation and control method for regulating and controlling late propagation of mandarin fish in autumn, and belongs to the field of artificial fish propagation in an aquaculture technology. The method comprises the following steps: (1) spawning induction in spring: injecting high-dose single oxytocin into mandarin fish parents; (2) completing partial oviposition under the action of high-dose single oxytocin, and realizing that the average oviposition amount of each female fish is 20-30% of the theoretical brood amount in spring; (3) carrying out bait control management from spawning to the end of June, reducing the feeding amount, and enabling the oviposition to be in a nutrition state of maintaining or slowly growing; (4) from July to August, carrying out nutrient enrichment cultivation, and recovering sufficient feeding in a period of time before formal spawning induction so as to promote rapid secondary development of gonads of the parent mandarin fish; spawning induction hormone is injected according to a conventional effective dose, artificial fertilization of the parent mandarin fish is induced, concentrated spawning is achieved, and the average spawning amount of each female fish is 80%-90% of the brood amount in autumn. According to the method, the mandarin fish parents can be used for two purposes in one year only through conventional hormone injection and feed management.

The utility model relates to the field of artificial insemination for pigs, and discloses a sanitary insemination tube for artificial insemination for pigs, which comprises a fixed tube, a push rod is slidably connected to the inner wall of the right end of the fixed tube, a fixed block is fixedly connected to the outer wall of the fixed tube, and a movable plate is slidably connected to the outer wall of the fixed tube. A guide rod is fixedly connected to the outer wall of the right end of the moving plate, a threaded rod is rotationally connected to the inner wall of the right end of the moving plate, and a sealing assembly is arranged on the inner wall of the left end of the fixing pipe and used for sealing an opening in the left end of the fixing pipe. According to the device, the threaded rod is rotated to drive the movable plate to move, the distance between the outer wall of the left end of the movable plate and the left end of the fixed tube is adjusted according to the depth of the left side of the seminiferous duct moving into the vagina of the sow, and after adjustment is completed, the left side of the seminiferous duct can quickly and accurately move to the semen releasing position in the vagina of the sow according to the position of the movable plate. The artificial fertilization of the sows with the same body type is facilitated.