26 results about "Artificial fertilization" patented technology

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep weight traits and application thereof. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep body weight traits, which corresponds to 35074122bp of the 11th chromosome of a sheep genome and has a basic group of C / T. The SNP molecular marker has remarkable correlation with the sheep weight, 6.11% of weight variance variation can be explained, and T is favorable allelic variation of sheep weight traits. The SNP molecular marker provided by the invention can be used for identifying sheep weight traits, distinguishing high-weight sheep from low-weight sheep, selecting a TT genotype for breed conservation during breeding, taking the TT genotype as a breeding sheep during breeding, excluding sheep with C allele during hybridization with other sheep, and performing artificial fertilization by adopting sperm of a TT genotype breeding ram. The breeding efficiency can be greatly improved, and sheep flock with weight advantages can be obtained.

The invention discloses an epigenetics method for improving the cryopreservation efficiency of sheep semen. The method comprises the following steps: extracting seminal fluid of Donflilien and Hu sheep hybrid F1-generation sheep, dividing the seminal fluid into a fresh group and a frozen group, carrying out somatic cell removal and small non-coding RNA extraction, screening out differentially expressed microRNAs by utilizing a Pandorah sequencing technology, analyzing a target gene and a signal channel of the microRNAs, and determining the seminal fluid of the Donflilien and Hu sheep hybrid F1-generation sheep. The small non-coding RNA related to sperm cryopreservation is found to mainly relate to key biological processes such as oxidative stress response and cell surface receptor signal channels. The screened differentially expressed small non-coding RNA is added into the frozen semen through methods such as in vitro chemical synthesis, so that the artificial fertilization conception rate of the frozen semen is remarkably increased.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

The application provides a mature rubber tree fertilization method based on nutrient circulation, which comprises the following steps: step one, establishing a relational expression Y i = litter decomposition + rainfall input + tree leaching + artificial fertilization; Y o = tree absorption + surface runoff; step two, using the soil nutrient balance principle, i.e. nutrient input Y i = nutrient output Y o , the above formula is brought into, and litter decomposition + rainfall input + tree leaching + artificial fertilization = tree absorption + surface runoff is obtained; step three, through nutrient calculation and data analysis, a function relationship between the recommended amount of fertilization Y f of the mature rubber tree and the tree age X is fitted, i.e. Y f =aX 2 +bX+c; the application solves the contradiction that the fertilization management does not meet the production demand caused by the problems such as single rubber tree fertilization formula, old diagnosis technology and difficult-to-determine test analysis index, and has good yield-increasing effect and production practicability.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The application provides a dairy cow mating auxiliary device, which comprises two symmetrically distributed side frames, a front frame capable of clamping a neck of a cow is fixed between one end of the two side frames, the other end of one of the side frames is provided with a clamping ring with a circular ring structure, the ring wall of the clamping ring is a hollow structure, the inner ring wall of the clamping ring is made of elastic rubber material, and the ring wall of the clamping ring is connected with a gas pressure adjusting device capable of adjusting the gas pressure in the ring wall. In the artificial insemination process, the labor intensity of the staff can be reduced without artificial hand pulling of the cow tail, and the stability of the cow tail clamped by the inner ring wall is relatively high, so that the cow tail cannot break away from the clamping ring even if the cow resists and swings the tail.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The present invention discloses a method for artificially inseminating eel eggs, comprising the following steps: 1. preparing eel semen and female eel eggs; 2. preparing an eel egg artificial insemination device and an egg incubation device; 3. adding eel semen and semen activation liquid to the artificial insemination device; 4. placing eggs in an egg receiving tray and placing it on a tray rack within the device, activating the device, extracting and atomizing eel semen so that it adheres to the surface of the eel eggs; 5. activating an activation unit and spraying activation liquid into the egg receiving tray to activate the eel sperm; 6. placing the fertilized eel eggs in the egg incubation device for incubation. The artificial fertilization method for eel eggs recorded in the present invention greatly shortens the migration distance of eel sperm after activation, allows the sperm to combine with the egg in a short time to complete fertilization, and the whole process does not touch the eel eggs, thereby greatly improving the fertilization success rate of the eggs.

The invention relates to a fertilizing device for agricultural cooperative planting, the fertilizing device comprises an unmanned aerial vehicle seat, a support member connected below the unmanned aerial vehicle seat and a wing member connected above the unmanned aerial vehicle seat, the unmanned aerial vehicle seat is connected with a fertilizing member, and the fertilizing member comprises a water spraying support connected to the unmanned aerial vehicle seat and a water spraying head connected to the water spraying support; the unmanned aerial vehicle seat is connected with a liquid fertilizer box. The technical scheme has the beneficial technical effects that the wing parts can drive the unmanned aerial vehicle base to flexibly move, a large-area planting area can be quickly covered, the problems that the cooperative planting area is wide, manual fertilization is time-consuming and labor-consuming, and the efficiency is low are effectively solved, the human input cost is reduced, and the fertilization operation period is shortened; the high-efficiency operation requirement of large-scale planting of a cooperative is met; the fertilizing part is composed of a water spraying support and a water spraying head and is matched with the liquid fertilizer box to continuously supply liquid fertilizer, and the liquid fertilizer can be evenly sprayed to the surfaces and root areas of crops.

The utility model discloses a fertilization device for strawberry planting, which comprises a drip irrigation pipe, a guide rail, chutes, driving lines A, a fertilizer spreading structure, a traction rope, a conveying pipe, a fertilizer back box and a box opening, the guide rail is fixed at the top of the side end of the drip irrigation pipe, the chutes are respectively arranged at the left end and the right end of the guide rail, and the driving lines A are distributed at the top end of the guide rail. The fertilizer spreading structure is connected to the guide rail in a sliding mode, the traction rope is installed on the fertilizer spreading structure, the bottom end of the conveying pipe is installed on the fertilizer spreading structure, the fertilizer back box is installed at the top end of the conveying pipe, and the box opening is formed in the top end of the fertilizer back box. The strawberry topdressing machine solves the problems that when strawberries planted in a ridging mode are subjected to topdressing, fertilizer is generally spread on ridge backs manually, it is avoided that the fertilizer is spread on strawberry seedling leaves as much as possible, and time and labor are wasted.

The utility model discloses an anti-reflux artificial fertilization tube, which comprises an injector and a catheter, the injector is composed of a push rod and a shell, the push rod is inserted into the shell, a piston is fixed at the tail end of the push rod and is in sliding fit with the shell, and at least one hole is formed in the catheter for seminal fluid to flow out; an anti-backflow assembly is additionally arranged between the injector and the guide pipe and comprises an external member, a first connecting groove is formed in one side of the external member, a first plug is fixed to one end of the shell, and the injector and the anti-backflow assembly are fixed by inserting the first plug into the first connecting groove. A first connecting groove is formed in one side of the sleeve piece, a second connecting groove is formed in the other side of the sleeve piece, the guide pipe is inserted into the second connecting groove to achieve fixation of the guide pipe and the backflow preventing assembly, the second connecting groove is communicated with the first connecting groove through a middle groove, an anti-overflow ring is fixed to the sleeve piece, and at least two anti-overflow pieces are fixed in the middle groove.

The utility model relates to a soil fertilizer quantitative fertilization control device which comprises a machine frame and a fertilizer cylinder arranged on the machine frame, walking wheels are installed outside the machine frame in a rotating mode, a plurality of sets of second through holes are formed in the bottom of the fertilizer cylinder, a rotating disc is further connected to the bottom of the fertilizer cylinder in a rotating mode, a plurality of sets of first through holes are formed in the rotating disc, and a plurality of second through holes are formed in the rotating disc. According to the lawn fertilizer applicator, fertilizer is poured into the fertilizer cylinder, then the lawn fertilizer applicator is pushed to move and walk on a lawn or a farm, the first through hole and the second through hole in the rotating disc can correspond to each other by adjusting the pulling unit, and therefore the fertilizer can leak out for fertilization; compared with manual fertilization, the fertilizer applicator is high in efficiency, easy and convenient to use.

The utility model relates to a frozen semen unfreezing water temperature accurate control device, and belongs to the technical field of artificial fertilization for livestock. The unfreezing water tank is embedded in the top of the shell, the electric heating plate is installed at the bottom in the unfreezing water tank, the motor is installed at the bottom in the unfreezing water tank, the stirring impeller is installed at the bottom in the unfreezing water tank, the motor is in transmission connection with the stirring impeller, the temperature sensing probe is installed in the unfreezing water tank, and the semen freezing pipe limiting device is installed in the unfreezing water tank. The plc is mounted in the electric appliance mounting cavity, and the control panel is embedded in the side wall of the shell, so that the change of the water temperature can be intuitively known; the temperature sensing probe, the control panel, the electric heating plate and the motor are connected with a plc (programmable logic controller); the top cover covers the top of the thawing water tank; when the temperature is too low, the water body is heated through the electric heating plate, and the water body temperature is kept within a proper range; thawing water is slowly stirred through the motor and the stirring impeller, water temperature uniformity is guaranteed, and the problem of large water temperature difference at different positions is solved. The semen thawing effect is improved, and the working efficiency is improved.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a normal-temperature sheep semen preservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in normal-temperature preservation and / or dilution of the sheep semen for the first time, and the semen quality of the sheep semen during normal-temperature preservation and / or dilution can be improved by the alpha-ketoglutaric acid or salt thereof; such as sperm motility rate, sperm motility, sperm motility performance, sperm viability, sperm plasma membrane intact rate, sperm acrosome intact rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm normal-temperature preservation diluent.

The invention discloses a method and a system for regulating and controlling synchronous reproduction of large yellow croakers by utilizing reproduction period barks. The method comprises the following steps: selecting healthy larimichthys crocea parent fishes from the end of the IV stage to the end of the V stage, pairing one female larimichthys crocea parent fishes and performing spawning induction; within 20-40 hours after spawning induction, acoustic stimulation signals obtained on the basis of mutals in the breeding period of the parent fishes are played to the parent fishes, and the signal characteristics include the main peak frequency of 163-673Hz, the pulse width of about 2ms, the interval of about 67ms and the sound pressure level of 116.6-123.4 dB re < 1 > mu Pa; after spawning is monitored, semen is immediately obtained for artificial fertilization. The system comprises a storage playing module and an underwater sound radiation device. According to the method, synchronous spawning and spermiation of the parent fishes under one-to-one pairing are effectively promoted, and the spawning rate and the fertilization rate are remarkably increased.

This invention relates to a method for regulating the late autumn breeding of mandarin fish, belonging to the field of artificial fish breeding in aquaculture technology. The operation of this invention is as follows: (1) Inducing spawning in spring by injecting a high dose of a single oxytocin into the mandarin fish parent stock; (2) Under the action of the high dose of the single oxytocin, partial spawning is completed, achieving an average spawning volume per female fish of 20%-30% of the theoretical spawning volume in spring; (3) After spawning until the end of June, controlled feeding management is carried out to reduce the amount of feed, so that the fish are in a nutritional state of maintenance or slow growth; (4) In July and August, nutritional enhancement cultivation is carried out, and sufficient feeding is restored for a period of time before formal spawning to promote the rapid secondary development of the gonads of the parent mandarin fish; oxytocin is injected at a conventional effective dose to induce artificial insemination of the parent mandarin fish, resulting in concentrated spawning, achieving an average spawning volume per female fish of 80%-90% of the spawning volume in autumn. This invention can achieve the dual use of mandarin fish parent stock in one year through conventional hormone injection and feed management.

The utility model is applicable to the technical field of agricultural planting, and provides a terrace red rice planting and fertilizing device which comprises an unmanned aerial vehicle main body for flying and moving fertilization, a mounting plate is fixed on the unmanned aerial vehicle main body, and a detachable storage box is arranged on the mounting plate and used for containing water and fertilizer liquid; the spraying cover is fixedly installed at the bottom of the storage box and used for spraying water and fertilizer to the red rice planted in the terraced field; the water pump is fixedly installed at the bottom of the storage box and used for pumping water and fertilizer, and the water inlet end of the water pump is fixedly communicated with the storage box through a liquid pumping pipe. According to the terraced field red rice planting and fertilizing device, the problems that existing terraced field red rice planting land is complex in terrain and large in gradient, so that mechanical land fertilization is inconvenient, and the manual fertilization efficiency is low are solved.

The present application relates to the field of genetic breeding of shellfish, and particularly relates to a method for artificially inducing triploid of Haliotis discus hannai. The method comprises the following steps: 1) promoting maturity and selection of seed abalones: selecting healthy seed abalones to promote maturity synchronously in a seed abalone culture pond, and selecting diploid abalones with good gonad development; 2) artificial spawning: performing spawning on the selected seed abalones until the female and male seed abalones spawn and ejaculate; 3) selection and mixed fertilization of gametes: filtering impurities in sperm and eggs respectively, mixing the sperm and the eggs to perform artificial fertilization, and obtaining zygotes; 4) heat shock induction: after 15 minutes of fertilization, placing the zygotes in seawater with a preheated temperature of 26-31 DEG C for 10-15 minutes to inhibit the discharge of the second polar body of the zygotes; and 5) incubation: immediately collecting the zygotes into an incubation tank after the heat shock to perform incubation. The triploid of Haliotis discus hannai obtained by the method has heat resistance, and can reduce economic losses of fishermen caused by difficulty of abalones in summer. By improving the spawning mode of seed abalones and optimizing the induction conditions, the triploid of Haliotis discus hannai can be obtained efficiently and stably.

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep weight traits and application thereof. The SNP molecular marker provided by the invention corresponds to the 159223939bp of the second chromosome of the sheep genome, and the basic group is A / G; wherein the basic group A is a favorable allelic variation of sheep body weight traits. The SNP molecular marker disclosed by the invention has obvious correlation with the sheep weight, and 5.14% of weight variance variation can be explained. The SNP molecular marker provided by the invention can be used for identifying the weight character of sheep, distinguishing sheep with superior weight character from sheep with inferior weight character, selecting an AA genotype for breed conservation during breeding, taking the AA genotype as a breeding sheep during breeding, excluding sheep with G allele when hybridizing with other sheep, and identifying the weight character of the sheep. Artificial fertilization is carried out by adopting semen of AA genotype breeding rams, the breeding efficiency can be greatly improved, and sheep flock with weight advantages can be obtained.

The invention relates to a culture regulation and control method for regulating and controlling late propagation of mandarin fish in autumn, and belongs to the field of artificial fish propagation in an aquaculture technology. The method comprises the following steps: (1) spawning induction in spring: injecting high-dose single oxytocin into mandarin fish parents; (2) completing partial oviposition under the action of high-dose single oxytocin, and realizing that the average oviposition amount of each female fish is 20-30% of the theoretical brood amount in spring; (3) carrying out bait control management from spawning to the end of June, reducing the feeding amount, and enabling the oviposition to be in a nutrition state of maintaining or slowly growing; (4) from July to August, carrying out nutrient enrichment cultivation, and recovering sufficient feeding in a period of time before formal spawning induction so as to promote rapid secondary development of gonads of the parent mandarin fish; spawning induction hormone is injected according to a conventional effective dose, artificial fertilization of the parent mandarin fish is induced, concentrated spawning is achieved, and the average spawning amount of each female fish is 80%-90% of the brood amount in autumn. According to the method, the mandarin fish parents can be used for two purposes in one year only through conventional hormone injection and feed management.

The present invention belongs to the field of shellfish genetic breeding technology and relates to a seed production method for improving the summer survival rate of northern oysters. Oysters primarily cultured in northern and southern my country are collected as base populations, and artificial insemination is used to construct a self-propagated population GG of the primarily cultured northern oysters and a hybrid population GA of the two oysters. The two populations are co-cultured in multiple northern sea areas, and their summer survival rates are tested. Based on the summer survival rates, individuals from the two populations cultured in a certain northern sea area are selected as parents. Artificial insemination is then used to construct populations GG and GG*GA, respectively. The two populations are co-cultured in multiple northern sea areas, and their summer survival rates are tested. Based on the ratio of the summer survival rates of the population GG*GA to the population GG in each sea area, individuals from the population GG*GA in the sea area with the highest ratio are selected as oyster germplasm with improved summer survival rate in northern China. The present invention can efficiently and quickly obtain oyster germplasm with significantly improved summer survival rate, significantly improving the summer survival rate and growth rate of cultured oysters in northern my country.

The utility model relates to the field of artificial insemination for pigs, and discloses a sanitary insemination tube for artificial insemination for pigs, which comprises a fixed tube, a push rod is slidably connected to the inner wall of the right end of the fixed tube, a fixed block is fixedly connected to the outer wall of the fixed tube, and a movable plate is slidably connected to the outer wall of the fixed tube. A guide rod is fixedly connected to the outer wall of the right end of the moving plate, a threaded rod is rotationally connected to the inner wall of the right end of the moving plate, and a sealing assembly is arranged on the inner wall of the left end of the fixing pipe and used for sealing an opening in the left end of the fixing pipe. According to the device, the threaded rod is rotated to drive the movable plate to move, the distance between the outer wall of the left end of the movable plate and the left end of the fixed tube is adjusted according to the depth of the left side of the seminiferous duct moving into the vagina of the sow, and after adjustment is completed, the left side of the seminiferous duct can quickly and accurately move to the semen releasing position in the vagina of the sow according to the position of the movable plate. The artificial fertilization of the sows with the same body type is facilitated.

The invention discloses application of salvianolic acid A in preparation of a medicine for improving the sperm function, and belongs to the technical field of biological medicine. SAA is polyphenol acid formed by caffeic acid and tanshinol, namely salvianolic acid through an ester bond and an enol bond, is a soluble component generated by many salvia species, is mainly extracted from salvia miltiorrhiza, and has the advantages that the salvianolic acid A is prepared from salvianolic acid A, salvianolic acid B and salvianolic acid C; sAA is one of the most abundant salvianolic acid in a salvia miltiorrhiza extract, a large number of studies show that SAA has a remarkable antioxidant effect, SAA can improve the function of in-vitro mature sperms, compared with a control group, after SAA is added, sperm intracellular calcium signals and hyperactivity are remarkably improved, and the occurrence rate of sperm acrosome reaction is also remarkably increased; the application of SAA can effectively improve the sperm function in the in-vitro fertilization process and improve the sperm motility of weak sperm patients, so that infertility is intervened and treated through simple non-invasive means such as artificial fertilization, and the fertility outcome of the patients is improved.

The invention discloses a fertilizing device for agricultural planting and a fertilizing method. The fertilizing device comprises a base, a mixing box is fixedly connected above the base, a convex column for pushing a stirring rod is fixedly connected in the mixing box, a spring is fixedly connected to the side face of the stirring rod, and a rotating frame is fixedly connected to the side, away from the stirring rod, of the spring; the rotating frame penetrates through the mixing box to be in transmission connection with a rotating column, the rotating column is fixedly connected with a second bevel gear, the second bevel gear is in meshed connection with a first bevel gear, the device automatically drills through an output gear, a rotating outer tooth cylinder and a drill bit, and the drilling time consumption of a single fruit tree is greatly shortened; aiming at the problem of uneven concentration of artificial fertilization, the device accurately controls the single-time fertilizer amount through a weighing plate and cooperates with the intermittent telescopic action of a stirring rod to ensure that chemical fertilizer and water are fully and uniformly mixed, and the water and fertilizer conveying rod dynamically distributes the fertilizer; the water and fertilizer concentration at each depth of soil is kept in a stable deviation range.

The invention provides an electrochemical biosensor for follicle monitoring, which comprises a sensing electrode with a porous structure and a hydrophilic insulating porous layer for blocking the sensing electrode to prevent short circuit, the sensing electrode comprises a porous substrate layer formed by taking a porous material as a substrate, a conductive layer formed by modifying a conductive material on the surface of the porous material, and an aptamer layer formed by modifying an aptamer corresponding to a follicle maturation related marker on the surface of the conductive material. The sensing electrodes are combined into a multi-layer sensing electrode array in a stacked mode, and a hydrophilic insulating porous layer is arranged between every two layers of sensing electrodes. The invention also provides a preparation method of the sensor and application of the sensor in preparation of a follicle monitoring device. According to the electrochemical biosensor, the maturity and quality of follicles are judged by comprehensively analyzing the relative concentrations and change trends of the multiple markers, the follicle monitoring accuracy is improved, dependence on professional large-scale equipment and professional technicians is not needed, operation is convenient, and clinical medication, artificial fertilization, follicle puncture and the like can be guided.

The utility model discloses a portable agricultural fertilization device, which relates to the technical field of agricultural fertilization and comprises a fixed seat, a feeding device is fixedly connected to the left upper part of the fixed seat, a punching device is fixedly connected to the right upper part of the fixed seat, the front side and the rear side of the fixed seat are both in threaded connection with two grips, and a motor drives a belt wheel to rotate. A belt wheel drives a first drill bit through a belt, the first drill bit drives a second drill bit to rotate, the second drill bit drives a limiting block to rotate, the limiting block drives an extension rod to rotate, the extension rod drives a sliding table to rotate, the sliding table drives a connecting rod to rotate, and the connecting rod drives a third drill bit to rotate; spiral grooves are formed in the outer walls of the first drill bit and the second drill bit, redundant soil can be brought upwards during punching, the second drill bit can press the third drill bit when rotating downwards, soil is prevented from entering the second drill bit, and the problems that manual fertilization is high in labor intensity and low in efficiency are solved.