253 results about "Artificial fertilization" patented technology

The invention relates to preserving liquid for sperm, in particular to the preserving liquid for swine sperm, and belongs to the technical field of animal reproduction. The preserving liquid for the swine sperm comprises the following components: 25.5 to 35.0g/l of anhydrous dextrose, 5.1 to 7.0 g/l of trisodium citrate, 1 to 10 g/l of sodium bicarbonate, 2 to 4g/l of EDTA, 1 to 3g/l of potassium chloride, 2.4 to 3.4g/l of citric acid, 8.5 to 9.5g/l of trihydroxymethylaminomethane, 0.26 to 1g/l of amikacin and 0.2 to 1g/l of vitamin C. Through detection, in the swine sperm preserved by the preserving liquid, the sperm viability reaches 0.60 to 0.85 and the integrity rate of sperm acrosome reaches 90 percent, which excess the requirement of the current artificial fertilization.

The invention discloses a method for artificial propagation of giant salamanders and cultivation of baby giant salamanders, which is characterized in that an ecological giant salamander farm is built in an original ecological region, the water in a cultivation pond comes from ravine streams of the original ecological region, the male giant salamanders are ecologically cultivated in classified ponds in different stages, the female giant salamanders are cultivated through indoor artificial cultivation, the male parent giant salamanders are specially cultivated by controlling water quality and water temperature and timely deploying nutrition, so that sperm motility of the male parent giant salamanders can be improved, and reproductive rate of the giant salamanders is further improved by performing selection, artificial hastened parturition and artificial fertilization on the parents; the propagated baby giant salamanders are cultivated in three stages, and disease prevention for the baby giant salamanders is performed in the feeding process. With the method of the invention, productivity of the artificially propagated giant salamanders can be more than 90%, the rate of artificial fertilization can be more than 80%, and hatchability can be more than 83.51%. By applying the method, population quantity of the giant salamanders can be improved, and scaled cultivation of the giant salamanders can be implemented.

A distant hydridization method between the subfamilies of cyprinid includes chealth eugamic male and female parents catalyzing female, artificial fertilization, incubation and naturalizing to obtain to descendant with strong bionergy. Its advantages are high disease resistance, high survival rate and high growth speed.

The invention provides a beef cattle breeding method. The beef cattle breeding method comprises the following steps that firstly, beef cattle of improved variety are selected; secondly, cows are raised; thirdly, a breeding method is conducted, wherein a, estrus induction is conducted on the cows close to the estrus, the cows are observed very carefully early in the morning and at nightfall, and observation is conducted every 4-5 hours in the daytime for estrus situation identification, and an outer observation method and a rectal palpation method for the follicular development degree are adopted as the estrus situation identification method, and b, artificial fertilization is conducted through a rectum grasping semen deposition method at estrus stage III; fourthly, the cows are kept under the precious care as long as pregnancy is confirmed according to the situation that the cows are not in estrus any longer within two to three months after semen deposition, or through rectum pregnancy check so that the cows can breed healthy calves, and if false pregnancy is found, re-copulation measures are taken in real time to reduce the breeding loss. By means of the method, the physique of the cows is improved, and the breeding rate of the beef cattle is further increased through estrus induction.

The invention discloses a method for breeding a high-quality mite-resistant bee species. The method is characterized by comprising the following steps of: (1) selecting breeding materials: screening a 'Jiangshan No.2' Italian bee with a high royal jelly yield, a French pure-bred Italian bee with excellent honey making performance and a healthy local Chinese bee species with excellent mite resistance for serving as an introduction object according to series indexes such as mite resistance, productivity, vitality and the like; (2) breeding a mite-resistant bee species: breeding a breeding male bee from Jiangshan No.2, breeding a Jiangshan No.2 virgin queen bee according to the requirements of bee biology and genetic breeding characteristic, and performing artificial fertilization hybridization with the bred breeding male bee to form a Jiangshan No.2 bee species with high production performance and disease resistance for serving as a male parent, wherein a pure-bred Italian bee with excellent honey making performance introduced from France serves as a female parent; and (3) checking and cutting a French Italian bee drone pupae every 7 days from breeding in spring till the end of breeding. The harm of bee mites to bee colonies can be avoided for beekeepers, the reproduction speed of the bee colonies is increased, and the yields and qualities of bee products are improved.

The invention relates to a semen freezing method for sheep, in particular to an ultra-high activity tubule semen freezing method for sheep. The method solves the problems that after the conventional frozen semen of sheep thaws, the motility rate and the activity are low, and the frozen effect is unstable. The method comprises the following steps of: filling fresh semen of sheep into the bottom part of a first centrifuging tube holding cleaning liquid; standing in a CO2 incubator for 30 to 60 minutes, wherein the semen with high activity goes up in supernatant; sucking the supernatant and thenfilling into a second centrifuging tube for centrifugation, wherein the semen with high density and high activity is obtained at the bottom of the second centrifuging tube; and sucking the condensed high-activity semen, adding into a frozen diluent, uniformly mixing and filling in tubules; performing program freezing and storing in liquid nitrogen, and thawing the stored semen before semen deposition. The sheep semen is subjected to high activity screening before freezing according to the method, and the motility rate of the thawing tubule frozen semen of sheep reaches 0.45 to 0.60, requirements on the artificial fertilization of the frozen semen of sheep are fully met, and the method can be widely applied to mass production of sheep.

The invention provides a goat reproduction method applicable to goat raising areas with fewer high-quality stock rams and larger goat flock quantity. The method can improve the mating efficiency of goats by multiple times and facilitates grazing fattening of nanny goats. The mating capacity of excellent stock rams can be developed sufficiently, the utilization of the rams is improved, improvement is accelerated, the feeding number of the stock rams can be reduced, the fodder cost can be saved, the conception rate of the nanny goats can be increased and infectious diseases and reproductive system diseases are prevented. An artificial fertilization mode is adopted, the infectious diseases among the goat flocks due to mating can be avoided, the conception rate of the nanny goats is increased, and the reproduction capacity of the goat flocks is improved.

The invention relates to a method for cultivating and artificial breeding plateau saline-alkali water area northern pike, aiming at providing a technology suitable for cultivating and artificial breeding the northern pike in the plateau saline-alkali water area. The method adopts first filial generation fingerling bred by wild northern pike to be cultivated into parent fish. The method is characterized in that before the northern pike fingerling is put into a pond, forage fish can be cultivated by an original pond, the northern pike fingerling with the length of 5cm is put into the pond aftertwo weeks, and the polyculture proportion between the northern pike fingerling and the forage fish is 1:200-300; when the temperature of the pond reaches to 7-8 DEG C, parent fish roe of the northernpike can be selected for semidry method artificial fertilization for 5min, and talc powder is used for abhesion after fertilization; oosperm is sterilized by malachite green solution and then enters into an incubator to be incubated; by controlling the speed and the flow rate of the water flow, the dead oosperm of the northern pike can be promoted to be discharged from a drain pipe; deep well water which contains 7-9mg/L of dissolved oxygen and has the water temperature of 12DEG C can be continuously pumped into the bottom of the incubator, and running water incubation can be carried out underthe dim light; and when the fingerling flatly swim to intake the exogenous nutrition, the fish can be fed by fish bait in time.

The invention discloses a method for artificial fertilization of sturgeon. The method comprises the following steps: checking the maturity of parent fish, collecting spermatic fluid, collecting fish roes, and carrying out artificial fertilization. Through the artificial fertilization method disclosed by the invention, the rate of fertilization is up to over 90%, and the fertilization of sperms and fish roes can be judged by naked eyes; and the unfertilized fish roes are removed, so as to greatly improve hatching rate. The artificial fertilization method effectively combines roe collection by squeezing and pressing and roe collection by in vivo manual incision, and is better than any one individual method. The disinfected parent fish of sturgeon can be re-placed into an incubation pond, and can regain health and recover ovulation capacity quickly.

The invention discloses a cultivation method of black-feather pink-egg layer chickens. The cultivation method comprises the following steps of: (1) selecting a brown-egg layer chicken great-grandparent A series cock, a brown-egg layer chicken great-grandparent B series cock, a brown-egg layer chicken grandparent A series cock, a brown-egg layer chicken grandparent B series cock or a brown-egg layer chicken parent AB series cock as male parents and extracting seminal fluid by using a manual mode; (2) selecting a highest ranking imperial concubine hen as a female parent to carry out artificial fertilization; (3) collecting hatching eggs laid by the highest ranking imperial concubine hen in two days after the fertilization and hatching the eggs; and (4) obtaining hatched hens namely the black-feather pink-egg layer chickens. The body shape, the weight and the appearance of the cultivated black-feather pink-egg layer chicken are similar to that of a native chicken, the color of the feathers of the cultivated layer chicken is black, and laid eggs have pink shells. The egg laying property of the cultivated layer chicken is good, the annual egg yield can reach 210-230, and the later egg weight is about 50g. The black-feather pink-egg layer chickens can be raised in cages or stocking, have high spent chicken price and are suitable for large-scale cultivation.

The invention discloses an artificial propagation method for Taiwan mud fishes and belongs to the propagation technologies for fresh water mud fishes. The method includes the following steps that (1), parent mud fish fries are selected; (2), artificial spawning induction is performed; (3), artificial fertilization is performed; (4), hatching is performed. According to the technology, the method that human chorionic gonadotropin (HCG), hypophyses or luteotropin releasing hormone d-ala analogs and other spawning induction medicine are injected to the backs of the parent mud fishes is adopted, so that the spawning induction rate of the parent mud fishes is increased over 91.0%; according to the technology, the fertilization rate of the parent mud fishes is increased over 96.6% through artificial fertilization, and the hatching rate of the parent mud fishes is increased over 98.0% through still water hatching; the survival rate of the parent mud fishes after artificial propagation reaches 90.9%. Propagation is performed by the utilization of the method, so that the survival rate, the spawning induction rate, the fertilization rate and the hatching rate of the Taiwan mud fishes are much higher than the corresponding rates through natural propagation and traditional propagation method for the mud fishes, industrialized production of the Taiwan mud fishes can be achieved, the fries are provided for large-scale farming, and high economical benefits are obtained.

A hybridization method between Chinese spiny sea cucumber and Russian (or korean) one for preparing the hybrid one with high growth speed, stress resistance, survival rate and nutritive value includes such steps as using special culture technique to make them mature symultaneously, artificial oxytocia to obtain their spermatozoa and ova, artificial fertilization, and culturing fertilizer ova until they become laval plankton.

A distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss relates to a distant hybridization method of cold water fishes. The invention resolves the problems of Oncorhynchus masou of nonsynchronous male and female maturing, low rate of emergence, bad adversity resistance, high mortality rate of ripe fishes after propagation, and the like, under the conditions of artificial cultivation. The steps of the inventive method are as follows: firstly, cultivations are respectively carried out, so that progenitive parents can be synchronously matured; secondly, artificial fertilization is carried out; thirdly, breeding is carried out. The method of the invention has stronger operability and good suitability, thereby avoiding the limitations of a hormone-induced method. The first filial generation of coenospecies cultivated by the invention has fast growing, tender meat, strong adversity resistance and high survival rate after cultivation, thereby having lower cultivation cost; compared with self cross of Oncorhynchus masou, the growth rate is increased by 10-15%, the survival rate is increased by 30%, the fertilization rate is above 90%, the hatchability rate is above 80%, after propagation, the mortality rate is below 8.7%, and the indexes of fleshcolor, tenderness, pH value, water loss rate and water holding capacity are all in accordance with the requirements of excellent-quality cold water fishes.

The invention discloses a culture method of barbus capito. The method comprises the following steps that barbus capito in rivers, lakes, reservoirs and other natural water is selected, or artificially cultured adult barbus capito is selected as parent fishes; a culture pond, the parent fish stocking number, the stocking mode and the culture and feeding mode are selected, and management is enhanced; the parent fishes are subjected to artificial spawning induction; artificial fertilization is carried out; artificial incubation is carried out; fry rearing is carried out; fingerling culture is carried out. Culture and screening of the parent fishes, the odinagogue variety, the concentration ratio, the injection mode, incubation conditions and other conditions are optimized, and the spawning induction rate, fertilization rate and incubation rate of the cultured barbus capito all reach 90% or above.

The method for breeding black rabbit includes the following steps: using local chesnut black male rabbit as male parent and using New Zealand female rabbit as female parent, makiing hydribization or artificial fertilization to obtain first-generation hybrid rabbit species, selecting excellent male rabbit and female rabbit to make direct cross or artificial fertilization to obtain second-generation hybrid rabbit species, then adopting similar method to obtain third-generation and fourth-generatin hybrid rabbit species, and utilizing fourth-generation male rabbit and female rabbit to make breeding. Said nvention adopts granular feed containing Chinese herbs of isatis leaf, lonicera flower, wild chrysanthemum flower and stemona root.

The invention discloses a culture-medium of pig semen. Per 100ml of the culture-medium contains the following components: 5-20 g of lactose, 0.005-0.05 g of N-acetyl-D-glucosamine, 0.05-0.5 g of DHA, 1.0-5.0 ml of glycerine, 0.2-1.5 ml of OEP, 10-40 ml of yolk and 50,000-200,000 IU of penicillin and/or streptomycin. The invention also discloses a method for processing the pig semen. Proved by detection, the activity of the pig semen processed by the method reaches 0.3-0.6, and the acrosome integrity exceeds 50 percent, thereby completely conforming to the requirement of artificial fertilization at present.

The invention relates to an artificial propagation and roe incubation method for freshwater fishes, in particular to an artificial propagation and roe incubation method for schizopygopsis malacanthus, and belongs to the technical field of artificial propagation and roe incubation of fishes. The artificial propagation and roe incubation method for schizopygopsis malacanthus mainly comprises the steps of parent fish selection, spawning induction, artificial impregnation, roe incubation and fry raising. The artificial propagation and roe incubation method for schizopygopsis malacanthus has the advantages that the fertilization rate can reach 89.6%, the incubation rate can reach 95%, and the problem of resource scarcity caused by environmental problems is resolved; the fry incubation process is convenient to conduct, the controllability is improved, and requirements for manpower and management in the incubation process are lowered.

The invention discloses a directional mating method for Penaeus vannamei Boone. The method includes the steps that parent prawns are selected and subjected to nutrient enrichment cultivation, a mating scheme is set, male prawns are ripened and independently cultivated, female prawns are ripened and independently cultivated, directional mating parent prawns are selected and subjected to directional mating, and spawning and fertilization are performed. The method is designed according to the life habits and the mating, fertilization and spawning characteristics of the Penaeus vannamei Boone, and possible injuries to the male prawns and the female prawns in the artificial fertilization process can be avoided. In the whole mating process, it is only needed to determine the serial numbers of the parent prawns for mating, operation is convenient and easy, and the genealogical relationship of established families can be accurately established. The genetic relationship of the individuals of the families is determined, the mating scheme is designed artificially, and inbreeding depression of the established families can be effectively avoided. The method has the advantages of being high in success rate and low in parent prawn loss, and overcomes the defects that in the prior art, when Penaeus vannamei Boone families are established in an artificial fertilization mode, spermatophore is prone to falling off, the fertilization rate is low, and half sibs cannot be established easily.

The invention provides an artificial sea bass breeding method suitable for river mouth areas. According to the method, the artificial sea bass breeding suitable for the river mouth areas is realized by virtue of the steps of parent sea bass selection and domestication, parent fish reproduction, fertilized ovum incubation, indoor fry cultivation, outdoor fry cultivation, fry screening and fry taking-out. The method is used for solving the key problems such as parent sea bass selection standards and domestication environment condition requirements, artificial parent fish ripening and spawning induction methods, artificial fertilization method, fertilized ovum incubation condition requirements, fry cultivation condition requirements, indoor and outdoor fry cultivation and bait transformation; the survival rate of sea bass fry stocking and the breeding efficiency can be remarkably improved; in short, the method is significant for the healthy development of the sea bass breeding industry in the river mouth areas of Pearl River Delta.

The invention discloses an artificial hybridization breeding method for epinephelus fuscoguttatus and epinephelus polyphekadion. The method comprises the steps of (1) choice of parents, (2) cultivation of the parents, (3) check and judgment of the gonad development degree of parent fishes, (4) artificial fertilization, (5) incubation of zygotes and (6) packing and transporting of the zygotes. Through the method, intergeneric hybridization of epinephelus fuscoguttatus and epinephelus polyphekadion is realized, first filial generation fish fry with characteristics of two parents is obtained and used for subsequent artificial breeding, and the cultivated hybrid fishes are good in breeding property, delicious in flesh and high in cost performance, and a new variety is provided for all fry varieties of fishes.

The invention discloses a water-fertilizer mixing device, comprising a mixing tank, a stirring device, a water inlet pipe, a fertilizer box and a drain pipe, wherein the stirring device is arranged on the mixing tank and is used for stirring materials in the mixing tank; the water inlet pipe is provided with a water suction pump; the fertilizer box is arranged on the mixing tank; a fertilizer conveyer for conveying fertilizer to the mixing tank is arranged in the fertilizer box; and the drain pipe is provided with a draining pump. A fertilizer solution is fully mixed with water before fertilization, so that even distribution of the fertilizer in various regions in a large field can be ensured, crops can evenly absorb nutrition, and the yield of the crops can be finally promoted to be balanced; meanwhile, water and the fertilizer are directly conveyed into an irrigation canal or a drip irrigation system of the large field through the draining pump after being mixed, and thus the process of artificial fertilization is removed, so that the labor intensity of people is lowered, and the fertilization efficiency is improved; and therefore, the fertilizing method is relatively suitable for large-scale planting of crops. The invention also provides a fertilization method of the water-fertilizer mixing device.

The invention provides an artificial multiplication method of epinephelus polyphekadion. The artificial multiplication method includes the steps of 1), parent selection; 2), parent cultivation; 3), inspection and judgment of gonad development degree of parent fish; 4), artificial fertilization; 5), hatching of fertilized eggs. Through optimization design of various phases of the epinephelus polyphekadion from parent cultivation to fertilization and cultivation fish fries after hatching of the fertilized eggs, fertilization rate reaches more than 99.2%, the fertilized eggs can be hatched to thefish fries in 24-26 hours, and hatching rate can reach more than 99.5%.

The invention discloses a method for carrying out incubation on neolissochilus benasi through artificial fertilization, which belongs to the technical field of fish product breeding. The method comprises the following steps of: injecting DOM (domperidone maleate) and LRH-A2 (lutein releasing hormone A2) into gonadosomatic neolissochilus benasi (1mg of DOM and 1 mu g of LRH per kilogram of neolissochilus benasi are injected in per kilogram of gonadosomatic neolissochilus benasi), wherein the injection volume is halved for male neolissochilus benasi, and the effect time is 15 hours at a water temperature of 22 DEG C; extruding ovums and sperms of the gonadosomatic neolissochilus benasi into a container, stirring the ovums and the sperms for 30 seconds by using chicken feather, then adding clear water at a temperature of 22 DEG C into the container (water preferably just submerges the ovums and the sperms), after stirring the obtained mixture for 20 seconds by using the chicken feather, carrying out washing three times by using clear water, then taking the obtained fertilized ovums for backup incubation; putting the fertilized ovums into an aqueous captan solution (0.15 ppm) to soak for 15 minutes, then putting the fertilized ovums into a water-filled dissecting tray and a shallow-water basin (in which the water level is preferably 3-5 cm higher than the ovums) for incubation, wherein 3000-5000 ovums with an ovum diameter of 2.2-2.8 mm are put in each basin, the position of a water-filled container is 50-80 cm higher than the dissecting tray and the shallow-water basin; sucking water by using a small-sized oxygen hose and conveying the water to the dissecting tray and the shallow-water basin to carry out incubation for 120 hours so as to get fish fries, wherein the temperature of the water is 22-23 DEG C, and the pH value of the water is 7.8-8.5. The method disclosed by the invention has the advantages of low cost, easiness in operation, and high incubation rate.