31 results about "Growing sheep" patented technology

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep weight traits and application thereof. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep body weight traits, which corresponds to 35074122bp of the 11th chromosome of a sheep genome and has a basic group of C / T. The SNP molecular marker has remarkable correlation with the sheep weight, 6.11% of weight variance variation can be explained, and T is favorable allelic variation of sheep weight traits. The SNP molecular marker provided by the invention can be used for identifying sheep weight traits, distinguishing high-weight sheep from low-weight sheep, selecting a TT genotype for breed conservation during breeding, taking the TT genotype as a breeding sheep during breeding, excluding sheep with C allele during hybridization with other sheep, and performing artificial fertilization by adopting sperm of a TT genotype breeding ram. The breeding efficiency can be greatly improved, and sheep flock with weight advantages can be obtained.

The invention relates to the technical field of modern livestock breeding, in particular to an SNP molecular marker YAP1 gene g.75896359AT related to the lambing traits of goats and application of the SNP molecular marker YAP1 gene g.75896359AT. The SNP molecular marker is located at the 51bp position of a nucleotide sequence shown in SEQ ID NO.01, and the polymorphism of the SNP molecular marker is A / T. When the genotype of the polymorphic site contained in the molecular marker is AT or TT, the molecular marker corresponds to the lambing trait relative advantage of the goat to be detected. The molecular marker provided by the invention can be applied to the identification or prediction of the lambing state of the goat, can also be applied to the field of molecular breeding, and judges the genetic potential of the goat based on the analysis of the genotype of the goat; by optimizing the dominant allelotype of the SNP molecular marker, the lambing performance of the progeny of the goat can be improved, the SNP molecular marker is used for genetic improvement of breeding sheep, and the economic benefit of the mutton sheep breeding industry is effectively improved.

The invention relates to the technical field of modern livestock breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker rs663564950 related to growth traits of goats and application of the SNP molecular marker rs663564950. The SNP molecular marker is located at the 51bp position of a nucleotide sequence shown in SEQ ID NO.01, and the polymorphism of the SNP molecular marker is C / T. When the genotype of the polymorphic site contained in the molecular marker is CT or TT, the genotype corresponds to the relative advantage of the growth traits of the goat to be detected. The molecular marker provided by the invention can be applied to the identification or prediction of the growth state of the goat, can also be applied to the field of molecular breeding, and judges the genetic potential of the goat based on the analysis of the genotype of the goat; by optimizing the dominant allelotype of the SNP molecular marker, the 6-month-old weight of the goat offspring can be improved, so that the SNP molecular marker is further used for genetic improvement of breeding sheep, and the economic benefit of the mutton sheep breeding industry is effectively improved.

The invention discloses a breeding performance prediction system based on an intelligent sheep breeding platform, and belongs to the technical field of animal husbandry informatization. The intelligent breeding platform is designed, standardized collection, storage and intelligent management of sheep full-life-cycle data are achieved, and a solid data foundation is laid for follow-up research; secondly, innovatively combining ensemble learning (AdaBoost, GBRT and the like), machine learning (SVR, KNN and the like) and a sorting learning algorithm, and constructing a multi-level breeding sheep breeding performance prediction model system; particularly, a subjective and objective combination weighting method based on AHP-PCA is provided, and the prediction precision of the model is remarkably improved through feature reconstruction; in addition, an Achimedes optimization algorithm (AOA) is introduced into the field of breeding prediction for the first time, and adaptive search of hyper-parameters is realized by using a physical simulation mechanism of the AOA, so that the accuracy, efficiency and robustness of the model are remarkably improved.

The application provides a molecular marker related to sheep growth traits and application thereof, the molecular marker is designed by primers according to the sequence of the LGALS3 gene, DNA is extracted from sheep blood, and through PCR amplification, DNA sequencing and sequence analysis, it is found that a T / C polymorphism site exists at the 298th position of the amplified fragment, further, the polymorphism site of 849 Hu sheep is detected by using AQP primers, a least square model is established, genotype and growth traits are associated, finally, it is determined that Y at the 298th bp of the amplified LGALS3 gene fragment shown in SEQ ID NO. 1 is T or C, the mutation leads to T / C polymorphism of the molecular marker, and the molecular marker can be used as a molecular marker related to sheep growth traits. The molecular marker of the application can be used for screening a new variety of fast-growing high-quality mutton sheep, and can be used for screening assisted breeding, cultivating fast-growing sheep, and helping to increase economic benefits.

The invention provides a multiplex digital PCR (Polymerase Chain Reaction) detection method for identifying and diagnosing Brucella bovis and Brucella mevis, i.e., on the basis of obtaining a set of primer probes capable of identifying Brucella and Brucella bovis and Brucella mevis through screening; the invention establishes a high-sensitivity liquid drop type digital PCR detection method capable of identifying zonal distribution Brucella and Brucella of cattle and sheep species. The method disclosed by the invention can be used for simultaneously carrying out quantitative detection on nucleic acids of the Brucella abortus and the Brucella melitensis, is good in repeatability and specificity, and in addition, the sensitivity is 10 times higher than that of a triple fluorescent quantitative PCR (Polymerase Chain Reaction) method. Clinical sample detection results show that the positive detection rate of the triple digital PCR detection method in environmental swabs, tissue samples, milk samples and abortion substances is higher than that of a triple qPCR method. The triple digital PCR detection method provided by the invention can be used for etiological diagnosis of cattle and sheep brucellosis, and has good sensitivity, specificity and repeatability.

The application provides a molecular marker related to residual feed intake of sheep, a detection method and application thereof. The application finds that there is an A / T polymorphic site at the 180th position of an amplified fragment by performing PCR amplification and sequence analysis on a BANK1 gene of sheep, further detects the polymorphic site of 1105 Hu sheep by using KASPar primers, establishes a least square model, and performs correlation analysis on the genotype and residual feed intake (RFI), and finally determines that the amplified BANK1 gene fragment can be used as a molecular marker related to residual feed intake of sheep. The application can be used for selecting and reserving sheep with TT homozygous gene into a core group as breeding sheep by detecting the molecular marker, so as to improve the residual feed intake of sheep and help to improve economic benefits.

The invention discloses an intelligent sheep phenotype measuring system based on computer vision. The system comprises a multi-view image acquisition array unit, an optimized support vector machine algorithm processing unit, a sheep body posture multi-dimensional resolving model construction unit and the like. According to the method, sheep body images are collected through a multi-view camera, data fusion analysis is carried out by utilizing an optimized support vector machine algorithm after feature extraction coding is carried out in combination with parameters such as varieties and ages, and all phenotypic parameters of sheep bodies are accurately measured and calculated by means of a sheep body posture multi-dimensional calculation model according to bone joint points and body surface contour features. According to the system, the defects of insufficient data utilization and inaccurate model description in the traditional technology are overcome, intelligent and efficient measurement of sheep body surface type parameters is realized, accurate data support is provided for scientific breeding decision and high-quality breeding sheep breeding, and the breeding efficiency and economic benefits of animal husbandry are effectively improved.

The invention discloses an intelligent construction system for rapid breeding of mutton sheep, and relates to the technical field of mutton sheep breeding, and the system comprises a data acquisition module, a gene analysis module, an intelligent decision module, a breeding execution module and a block chain transaction module. The data acquisition module is used for acquiring physiological data, gene data and behavior data of mutton sheep in real time through a sensor and a'Anhui sheep No.1 breeding chip '; the gene analysis module is used for carrying out accurate genotype analysis and gene scoring by utilizing the collected gene data; the intelligent decision-making module generates an optimal breeding scheme through a multi-objective optimization algorithm; the breeding execution module performs automatic hybridization, breeding management and offspring monitoring according to the intelligent decision result; and the block chain transaction module realizes automatic matching and decentralized transaction of breeding sheep resources through an intelligent contract. According to the invention, the health state of the sheep flock can be monitored in real time, the breeding decision is optimized, the manual intervention is reduced, the production efficiency is improved, and the transparency and automation of sheep transaction are realized.

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

The application provides a molecular marker related to a body size trait of sheep, a detection method and application of the molecular marker, and a nucleotide sequence of the molecular marker is shown as SEQ ID NO. 1, wherein Y at the 327th position is T or C. The application performs PCR amplification and sequence analysis on a PLEC gene of a Hushe sheep, finds that a T / C polymorphic site exists at the 327th position of an amplified fragment, i.e. the 23157th position of the PLEC gene, further detects the polymorphic site of 1313 Hushe sheep by using KASPar primers, establishes a least square model, and performs correlation analysis on genotypes and growth traits, and finally determines that the amplified CC gene fragment can be used as a molecular marker related to the body size trait of sheep. By detecting the molecular marker, the application can be used for selecting and reserving a sheep with a CC homozygous gene into a core group as a breeding sheep, so as to improve the body size trait of the sheep and help to increase economic benefits.

The breeding method comprises the following steps: S1, selecting a target, S2, establishing a core group, screening individuals meeting + / -20% of the target from a sensitive down producing goat group, and establishing a basic ewe group with more than or equal to 200 ewes in a male-female ratio of 1: (25-50); s3, carrying out genetic evaluation and matching; S4, carrying out molecular marker-assisted breeding; S5, carrying out efficient breeding management; S6, carrying out barn feeding facility and breeding specification; by means of the cultivation and efficient feeding method for the barn-feeding cashmere goats, the fineness of cashmere of adult male and female barn-feeding cashmere goats is smaller than or equal to 16 micrometers, the breeding rate of the female goats is larger than or equal to 180%, the weight of the female goats is larger than or equal to 45 kg, the cashmere yield is larger than or equal to 1000 g, the weight of rams is larger than or equal to 60 kg, the cashmere yield is larger than or equal to 1500 g, the comprehensive cultivation benefit is improved by 15-20%, industrial application of barn-feeding cashmere goat high-quality breeding sheep creation is achieved, and the economic benefits of the barn-feeding cashmere goats are improved. The feeding management level of barn feeding breeding is improved, and the industrial high-quality development of ecological environment quality control is achieved.

The application relates to the field of modern livestock breeding technology, and particularly relates to a SNP molecular marker related to a goat lambing number character PDE1A The SNP molecular marker is located at the 51st bp of the nucleotide sequence shown in SEQ ID NO. 01, and the polymorphism is A / T. When the genotype of the polymorphism site contained in the molecular marker is AA, the corresponding relative advantage of the lambing number character of the goat to be detected is obtained. The molecular marker provided by the application can be applied to identification or prediction of the goat lambing character, and can also be applied to the field of molecular breeding. Based on analysis of the goat genotype, the genetic potential of the goat is judged. By selecting the superior allele genotype of the SNP molecular marker, the application can improve the goat lambing number, is used for genetic improvement of breeding performance of the goat, and effectively improves the economic benefit of the goat breeding industry.

The invention relates to the technical field of modern livestock breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker rs637601961 related to the body weight of a goat at the age of 6 months and an application of the SNP molecular marker rs637601961. The SNP molecular marker is located at the 51bp position of a nucleotide sequence shown in SEQ ID NO.01, and the polymorphism of the SNP molecular marker is G / T. When the genotype of the polymorphic site contained in the molecular marker is GT or TT, the genotype corresponds to the relative advantage of the 6-month-old body weight of the goat to be detected. The molecular marker provided by the invention can be applied to the identification or prediction of the growth state of the goat, can also be applied to the field of molecular breeding, and judges the genetic potential of the goat based on the analysis of the genotype of the goat; by optimizing the dominant allelotype of the SNP molecular marker, the 6-month-old weight of the goat offspring can be improved, so that the SNP molecular marker is further used for genetic improvement of breeding sheep, and the economic benefit of the mutton sheep breeding industry is effectively improved.

The invention provides a primer group and a kit for detecting four bacterial abortion diseases of sheep, belongs to the technical field of biological detection, establishes a multiplex PCR (polymerase chain reaction) detection method for sheep brucellosis, sheep abortion salmonellosis, listeriosis and chlamydia disease and a kit thereof, and has the characteristics of high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like. The rapid differential diagnosis of four sheep bacterial abortion diseases can be realized through one-time PCR amplification, and the method has important advantages in the field of sheep disease diagnosis and identification.

The application discloses a kind of over rumen aureomycin premix for sheep digestive tract infection and preparation method, including capsule material and aureomycin premix, wherein, capsule material mass percentage is 45%-55%, and aureomycin premix mass accounts for 45%-55% of total mass.The premix of the application can avoid the degradation of aureomycin in sheep rumen, ensure sufficient active drug to reach intestinal lesions, and has excellent antibacterial and antidiarrheal effects on lamb dysentery, growing sheep stress bacterial enteritis and adult sheep digestive tract secondary bacterial infection, while reducing the dosage and reducing the risk of drug residues.

The present application relates to the modern livestock breeding technical field, especially to a SNP molecular marker YAP1 gene g.75896359AT related to goat lambing traits and application. The SNP molecular marker is located at the 51bp of the nucleotide sequence shown in SEQ ID NO.01, and the polymorphism is A / T. When the genotype of the polymorphism site contained in the molecular marker is AT or TT, the relative advantage of the lambing traits of the goat to be detected is obtained. The molecular marker provided by the present application can be applied to the identification or prediction of the goat lambing state, and can also be applied to the field of molecular breeding. Based on the analysis of the genotype of the goat, the genetic potential of the goat is judged. By selecting the superior allele genotype of the SNP molecular marker, the lambing performance of the offspring of the goat can be improved, which is used for genetic improvement of the breeding goat, and effectively improves the economic benefit of the mutton sheep breeding industry.

The utility model relates to the technical field of sheep house, and disclose a kind of improved house for Hainan sheep gene breeding, including sheep house body, the upper of sheep house body is fixedly connected with glass roof, the middle part of glass roof upper surface is fixedly connected with receiving shell, receiving cavity is set in the inside of receiving shell, one side of receiving shell outer wall is connected with power mechanism, the output end of power mechanism is connected with transmission assembly, the both ends of transmission assembly are all through the side wall of receiving shell to the inside of receiving cavity, and are respectively connected with sunshade component, the other end of two sunshade components is all through the inside wall of receiving shell, and extend to the end of glass roof. This kind of improved house for Hainan sheep gene breeding, when needing to provide illumination for Hainan sheep inside sheep house body, just need to drive transmission assembly to rotate by power mechanism, two sunshade components located in the inside of receiving cavity of receiving shell can be recovered, so that the illumination outside can be penetrated to the inside of sheep house body through glass roof.

The present application relates to the modern livestock breeding technical field, especially to a SNP molecular marker rs637601961 related to the 6-month weight of a goat and an application thereof.The SNP molecular marker is located at the 51bp of the nucleotide sequence shown in SEQ ID NO.01, and the polymorphism thereof is G / T.When the genotype of the polymorphism site contained in the molecular marker is GT or TT, the corresponding 6-month weight of the goat to be detected has a relative advantage.The molecular marker provided by the present application can be applied to the identification or prediction of the growth state of a goat, and can also be applied to the field of molecular breeding, the genetic potential of a goat is judged based on the analysis of the genotype of the goat, and the 6-month weight of the offspring of the goat can be improved by selecting the advantage allele genotype of the SNP molecular marker, and the genetic improvement of the breeding goat is further used, and the economic benefits of the mutton sheep breeding industry are effectively improved.

The invention provides a digital intelligence breeding sheep genetic evaluation and cultivation management system, which collects individual physiological, behavior and environmental data in real time through RFID, an intelligent necklace and automatic weighing equipment, uploads the data to a cloud after edge preprocessing, performs genomic breeding value GEBV evaluation by adopting an SSGBLUP model in combination with pedigree, phenotype and 40K SNP genotype data, and provides a new breeding sheep genetic evaluation and cultivation management system. And an XGBoost algorithm is fused to improve the complexity prediction precision. The cloud intelligent module realizes oestrus early warning, health monitoring, accurate feeding and breeding optimization, analyzes a multi-target breeding decision through an NSGA-II algorithm, and ensures that key data cannot be tampered and the whole process is traceable in combination with a block chain technology. According to the system, the seed selection accuracy, the breeding efficiency and the breeding intelligence level are remarkably improved, and the system is suitable for digital transformation and genetic improvement of a large-scale sheep farm.

The invention provides a molecular marker related to the tail fat weight character of Hu sheep and application of the molecular marker. The molecular marker is characterized in that a C / T polymorphic site exists at the 157th site of an IGFBP3 gene segment, such as SEQ ID NO.1, and genotypes of polymorphic sites of 862 Hu sheep are detected and a least square model is established by using a KASPar primer; correlation analysis is carried out on the genotype and the tail fat weight, and it is determined that the polymorphic site can be used as a molecular marker of the tail fat weight related traits of the lake-lake sheep. The tail fat weight is hereditary character, by measuring the tail fat deposition capacity of the breeding sheep, excellent individuals with low tail fat proportion can be screened as parents, and a low-fat and high-yield Hu sheep strain is gradually established. The tail fat weight of the Hu sheep carrying the CT genotype is obviously lower than that of CC and TT genotype individuals. The molecular marker can be used for screening CT genotype Hu sheep to enter a core breeding group, tail fat deposition is reduced, and carcass quality and breeding economic benefits are improved.

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep weight traits and application thereof. The SNP molecular marker provided by the invention corresponds to the 159223939bp of the second chromosome of the sheep genome, and the basic group is A / G; wherein the basic group A is a favorable allelic variation of sheep body weight traits. The SNP molecular marker disclosed by the invention has obvious correlation with the sheep weight, and 5.14% of weight variance variation can be explained. The SNP molecular marker provided by the invention can be used for identifying the weight character of sheep, distinguishing sheep with superior weight character from sheep with inferior weight character, selecting an AA genotype for breed conservation during breeding, taking the AA genotype as a breeding sheep during breeding, excluding sheep with G allele when hybridizing with other sheep, and identifying the weight character of the sheep. Artificial fertilization is carried out by adopting semen of AA genotype breeding rams, the breeding efficiency can be greatly improved, and sheep flock with weight advantages can be obtained.

The invention provides a milk goat female animal intelligent breeding method based on multi-source heterogeneous data fusion, and the method comprises the steps: collecting the body shape character, pedigree information, genome molecular marker data and breeding environment parameters of milk goat female animals, and constructing a milk goat female animal breeding multi-source heterogeneous database; performing breeding analysis on the dairy goat female livestock according to the database to obtain an estimated breeding value; constructing a milk goat female livestock breeding character two-way multi-task prediction model, and performing combined prediction on the milk goat female livestock breeding character parameters based on the database to obtain predicted breeding character parameters; and constructing a comprehensive selection index model according to the estimated breeding value and the predicted breeding character parameters to generate a dairy goat female livestock breeding recommendation result. According to the method, a comprehensive genetic evaluation system is established through multi-source heterogeneous data of dairy goat female animals, the screening efficiency of excellent female animals and the accuracy of female animal breeding values can be improved, and the genetic improvement period can be shortened, so that a scientific basis is provided for breeding sheep selection and elimination.

The invention relates to the technical field of livestock breeding tools, in particular to a breeding sheep breeding lactation device which comprises a wall hanging plate, a fixing frame is fixedly connected to the wall hanging plate, a lactation box is rotatably connected to the fixing frame, an isolation plate is arranged in the lactation box in a sliding fit mode, a transmission mechanism is rotatably connected in the lactation box, and a moving pipe is arranged on the transmission mechanism. A scrubbing frame is rotatably connected to one end of the moving pipe, two moving frames are symmetrically arranged on the moving pipe, a nipple cleaning mechanism is rotatably connected to the moving pipe, and by arranging the scrubbing frame and the nipple cleaning mechanism, after each time of lactation, clean water can be injected into the lactation box, and the inner wall of the lactation box can be cleaned through the rotating scrubbing frame; meanwhile, the nipple brush inserted into the nipple can be driven to rotate, so that the inner wall of the nipple is brushed, and the feeding device for breeding sheep breeding can achieve automatic cleaning, reduce manual intervention, improve the sanitary level and reduce the risk of bacterial contamination.

The present invention provides a molecular marker associated with sheep growth traits and its application. By performing PCR amplification and sequence analysis on the sheep FADS3 gene, the present invention discovered an A / C polymorphic site at position 111 of the amplified fragment. KASPar primers were further used to detect the polymorphic site in 1,195 sheep and establish a least-squares model to conduct an association analysis between genotype and growth traits. Ultimately, it was determined that the FADS3 gene fragment amplified by the present invention can serve as a molecular marker associated with sheep growth traits. By detecting this molecular marker, the present invention selects sheep homozygous for the AA gene to enter the core herd as breeding sheep, thereby improving the sheep's growth traits, shortening the breeding process, and contributing to increased economic benefits in the breeding industry.

This invention provides a molecular marker associated with growth traits in Hu sheep and its application. The nucleotide sequence of this molecular marker is shown in SEQ ID NO.1, where M at position 355 bp represents A or C. This invention, through PCR amplification and sequence analysis of the GC gene in Hu sheep, discovered an A / C polymorphic site at position 355 of the amplified fragment. Further analysis using KASPar primers to detect the polymorphic site in 1228 Hu sheep and establishing a least-squares model, along with genotype-growth trait association analysis, ultimately determined that the amplified GC gene fragment can serve as a molecular marker associated with average daily weight gain and feed conversion ratio in Hu sheep. This invention, by detecting the molecular marker, can be used to select homozygous AA-type Hu sheep for breeding stock in the core flock, thereby improving growth traits and increasing economic benefits.

The utility model relates to the technical field of breeding isolation fences, and discloses a breeding sheep breeding isolation fence. The guardrail comprises a guardrail corner column, a bolt cylinder is movably sleeved with the guardrail corner column, a first reset spring is connected to the top of the bolt cylinder, an L-shaped bolt column is connected to the outer portion of the bolt cylinder, a round bolt ring is movably sleeved with the outer portion of the L-shaped bolt column, and a guardrail connecting plate is fixedly connected to the outer portion of the round bolt ring. The exterior of the handrail connecting plate is connected with the isolation guardrail, the exterior of the isolation guardrail is fixedly connected, and the exterior of the isolation guardrail is movably connected with the metal pedal. According to the guardrail connecting plate, the bolt cylinder is arranged, so that the circular bolt ring can be conveniently reset into the circular ring of the circular bolt ring through the elastic force of the first reset spring, and the L-shaped bolt column and the circular bolt ring can be quickly separated when an operator disassembles and assembles the guardrail connecting plate welded with the isolation guardrail.

The application provides a molecular marker related to sheep growth traits, a detection method and application thereof. The application finds that a C / T polymorphic site exists in an amplified fragment of a sheep MKI67 gene through PCR amplification and sequence analysis of the sheep MKI67 gene, further detects the polymorphic site of 935 Hu sheep using KASPar primers, establishes a least square model, and performs correlation analysis on genotypes and growth traits, and finally determines that the amplified MKI67 gene fragment can be used as a molecular marker related to sheep growth traits. The application is used for selecting and retaining sheep with TT homozygous genes as breeding sheep for breeding, so as to improve the growth traits of sheep and help improve the economic benefits of the production and breeding industry.