The invention provides a molecular marking method for
sheep wool curl, which relates to a molecular marking method, which comprises the following steps of: 1, extracting the sheep
genome deoxyribonucleic acid (
DNA) for carrying out
polymerase chain reaction (PCR) amplification and then carrying out
enzyme digestion to obtain
enzyme digestion products; 2, carrying out
electrophoresis separation onthe
enzyme digestion products and carrying out
genotype judgment according to the
electrophoresis separation results; 3, carrying out association analysis and evaluating least square mean value of the property to obtain a result that the
wool curl of individuals with the TT
genotype type and TC
genotype in the three genotypes is obviously higher than the
wool curl of individuals with the CC genotype; and 4, dividing experiment groups into three types according to the result in the third step to complete the operation of the molecular marking method. The molecular marking method can be used for
wool curl and
fineness molecular marking auxiliary culture, the early period selection of the breeding sheep can be realized, and the sheep can be selected after the birth, so the breeding cost can be reduced, the breeding process of high-quality fine wool sheep is accelerated, the operation is simple, the accuracy is high, the efficiency is high, and the automatic detection can be realized.