35 results about "Allelotype" patented technology

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The invention relates to the technical field of biology, in particular to an SNP molecular marker for identifying upland cotton fiber quality, a KASP primer group and application of the SNP molecular marker and the KASP primer group. The invention provides five SNP (Single Nucleotide Polymorphism) molecular markers XW1-XW5 which are obviously related to fiber quality, and accurate and stable genotyping can be realized through verification. Wherein the fiber length can be remarkably increased by excellent allelotypes corresponding to the markers XW1, XW2, XW4 and XW5; the excellent allelotype corresponding to the marker XW3 can significantly improve the micronaire value, provides a reliable tool for upland cotton fiber quality identification and molecular breeding, and has important application value.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention relates to the technical field of modern livestock breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker rs663564950 related to growth traits of goats and application of the SNP molecular marker rs663564950. The SNP molecular marker is located at the 51bp position of a nucleotide sequence shown in SEQ ID NO.01, and the polymorphism of the SNP molecular marker is C / T. When the genotype of the polymorphic site contained in the molecular marker is CT or TT, the genotype corresponds to the relative advantage of the growth traits of the goat to be detected. The molecular marker provided by the invention can be applied to the identification or prediction of the growth state of the goat, can also be applied to the field of molecular breeding, and judges the genetic potential of the goat based on the analysis of the genotype of the goat; by optimizing the dominant allelotype of the SNP molecular marker, the 6-month-old weight of the goat offspring can be improved, so that the SNP molecular marker is further used for genetic improvement of breeding sheep, and the economic benefit of the mutton sheep breeding industry is effectively improved.

The invention relates to a computer-implemented method for determining pharmacogenetic star alleles from high-throughput sequencing data, the method comprising the following steps: providing a plurality of result files which have been output by a plurality of different computer programs for genotyping and which each have a plurality of result elements, wherein each result element has at least one gene designation; shortening the allele designation as far as possible on the right for each result element of the plurality of result elements from each result file of the plurality of result files; and outputting the gene designation and a specific result diplotype. The invention also relates to a computer-implemented method for determining rare genetic variants. Finally, the invention also relates to a device having a processor and a memory in order to carry out such methods.

The application discloses a genetic marker linked with a wheat kernel nickel ion accumulation QTL qNi.hnaas-6BS, adopts a Wheat Breeders 660K microarray chip to identify genotype data of a natural population, combines phenotype data of kernel nickel ion content of the wheat natural population, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BS for controlling wheat kernel nickel ion accumulation exists on a short arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP6872, is located at the 125,482,475th nucleotide of the 6B chromosome, and the SNP6872 has C / T polymorphism. Haplotype analysis results on a population level show that when the nucleotide of the site is CC, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype for limiting the accumulation of the wheat kernel nickel ion.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to thousand seed weight character of brassica napus and a KASP (Keyhole Associated Specific Protein) marker primer group thereof, and belongs to the field of gene engineering, the SNP marker is positioned at the 3967014bp position of the A09 chromosome of brassica napus, the reference genome version is ZS11.v0, the SNP marker has G / A polymorphism, A is an excellent allele type, the phenotype contribution rate of the A to thousand seed weight is 10.0-12.8%, and the KASP marker primer group has the advantages that the KASP marker primer group can be used for detecting the thousand seed weight character of the brassica napus; the individual with the genotype AA or AG at the position has a higher thousand seed weight character than the individual with GG. A specific KASP marker primer group is developed aiming at the SNP locus, and the KASP marker primer group can realize genetic typing of the locus, and can be used for molecular marker-assisted breeding selection, acceleration of thousand seed weight character identification and breeding in the rape breeding process, and improvement of breeding screening efficiency.

The present invention provides a method for improving rice yield tms5 The method for the sterility threshold temperature of two-line sterile lines comprises the following steps: TMS5 CRISPR / Cas9 gene editing targets T501 and T502 were designed 22bp and 57bp downstream of the gene translation start site ATG, respectively, and gene editing vectors TMS501 and TMS502 were constructed to transform rice varieties to achieve TMS5 Site-directed mutation of genes to obtain different multiple alleles tms5 Gene frameshift mutant material. Experiments have shown that in the same genetic background, the T501 target site editing tms5-1 The sterility starting temperature of the frameshift mutants was significantly higher than that of the T502 target. tm5-2 Mutants. The method provided by the present invention can quickly obtain rice with different sterility threshold temperature phenotypes under the same genetic background. tms5 Mutants can overcome the interference of different genetic backgrounds and provide a basis for studying rice tms5 The molecular mechanism and genetic regulatory network of the starting temperature of two-line sterility provide new ideas.

The application discloses a molecular marker related to wheat kernel nickel ion accumulation, uses a natural population constructed by a team in the early stage, uses a Wheat Breeders 660K microarray chip to identify genotypic data of the natural population, combines phenotypic data of the wheat natural population kernel nickel ion content, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BL controlling the wheat kernel Ni ion accumulation exists on a long arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP4008, is located at the 693,584,519th nucleotide of the 6B chromosome, and the SNP4008 has A / G polymorphism. Haplotype analysis results on the population level show that when the nucleotide of the site is AA, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype; when the nucleotide of the site is GG, the wheat kernel has higher nickel ion content, and is an unfavorable allelic genotype.

The invention belongs to the field of rice molecular genetics, and particularly discloses an MBKbase database-based dominant allelotype analysis method for stem strength genes, which comprises the following steps: firstly, analyzing different genotypes of rice stem strength genes SCM3 and OsRLCK191, and according to different genotype germplasm resources of the rice stem strength genes carried in MBKbase-Rice, constructing a dominant allelotype of the rice stem strength genes SCM3 and OsRLCK191; the method comprises the following steps: selecting rice materials with single-dominant allelic genes, double-dominant allelic genes and non-dominant allelic genes (reference genome type), determining the stem strength character of rice germplasm resources of different genotype combinations of the rice stem strength gene, and finally performing sequencing verification on different genotypes of the rice stem strength gene. Finally, clearly breeding a strategy that the stalks are thick and strong, the stalks are high in elasticity or the stalks are moderate in thickness and elasticity to improve the lodging resistance of the rice variety; therefore, low-cost and high-throughput genotype screening can be realized.

The invention discloses a protein GIP1 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 3 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 3, is related to plant grain size regulation and is derived from the sequence 3. The GIP1 gene disclosed by the invention can control the grain size character in rice, namely overexpression of the gene can increase the length and thousand grain weight of rice grains. According to the invention, a new gene resource is provided for research on the grain size character of plants, especially rice, and a rice material with a GIP1 excellent allelotype is selected for genetic improvement of rice and plays an important role in application in the field of rice breeding.

The application discloses a kind of rice Hot1 Gene and its alleles, molecular markers and applications belong to the technical field of molecular biology.The application clones high-temperature-resistant gene Hot1 In rice, through overexpression experiment, it is proved that the gene positively regulates the high-temperature-resistant ability of rice, and subsequent haplotype analysis shows that the alleles Hot1 Derived from Indian rice Kasalath Hot1-Hap7 Can significantly improve the high-temperature-resistant ability of plants after being introduced into japonica rice Nip.The application screens an allelic form of high-temperature-resistant gene Hot1 Through high-temperature treatment and agronomic trait analysis, it is proved that the allelic form has a significant contribution to the high-temperature resistance of rice, and provides an important gene resource for rice high-temperature-resistant breeding.

The invention provides development and utilization of a wheat pre-harvest sprouting resistance KASP molecular marker, and belongs to the technical field of molecular markers. The invention provides a segment containing a wheat head sprouting resistance SNP (Single Nucleotide Polymorphism) site, and the SNP site is positioned on a 675362746bp base on a wheat 7A chromosome and has G / A polymorphism. The wheat pre-harvest germination resistance character is researched through whole genome correlation analysis, correlation analysis is carried out in combination with 90k chip detection genetic typing data, and a new pre-harvest germination resistance site Qphs.hbaas-7A. 2 is obtained through screening. A molecular marker is developed by using the shown site, and a wheat population is verified to find that the G genotype is a favorable allelotype. A KASP typing method is designed on the basis of the molecular marker, and the pre-harvest sprouting resistance of wheat can be judged according to the fluorescence color through a fluorescent quantitative PCR method.

The invention discloses a protein TaEOG1 related to wheat grain quality regulation and control as well as a coding gene and application of the protein TaEOG1. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed quality regulation and is derived from the sequence 1. The TaEOG1 gene disclosed by the invention can control grain quality characters in wheat, namely homozygous mutation of the gene causes increase of protein content and precipitation value of wheat seeds. A new gene resource is provided for research on quality characters of plants, especially wheat grains, a wheat material with the TaEOG1 excellent allele type is selected for genetic improvement of wheat, and the gene plays an important role in application in the field of wheat breeding.

The invention belongs to the technical field of plant genetic engineering, and discloses an application of a gene Gohi.A03G043500 in salt-tolerant breeding of cotton. The invention discloses the expression characteristic of stress-induced down-regulation of the gene in the salt-tolerant cotton and the key function of the gene as a stress negative regulation factor for regulating growth-stress resistance balance for the first time. The salt tolerance of the cotton germplasm can be accurately identified by detecting the expression change of the gene under salt stress or the allelotype of a regulation region of the gene; meanwhile, the salt tolerance of the cotton can be effectively improved by accurately regulating and controlling the expression level of the gene. The invention provides a new functional marker, a breeding strategy and a gene resource for salt-tolerant breeding of cotton, and has important application value in breeding of salt-tolerant cotton varieties.

The invention discloses a protein LBL2 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant grain size regulation and is derived from the sequence 1. The LBL2 gene disclosed by the invention can control the grain size character in rice, namely overexpression of the gene can increase the length and thousand grain weight of rice grains. According to the invention, a new gene resource is provided for research on the grain size character of plants, especially rice, and a rice material with an excellent LBL2 allele type is selected for genetic improvement of rice and plays an important role in application in the field of rice breeding.

The invention discloses a genetic marker linked with wheat grain nickel ion accumulation QTL qNi.hnaas-6BS. The genetic marker is characterized in that genotype data of a natural population is obtained through identification by adopting a Wheat Breeds 660K # imgabs0 # microarray chip, and genotype data of the grain nickel ion content of the natural population of wheat is combined to carry out whole genome association analysis (GWAS), so that the genetic marker linked with the wheat grain nickel ion accumulation QTL qNi.hnaas-6BS can be obtained, and the genetic marker linked with the wheat grain nickel ion accumulation QTL qNi.hnaas-6BS can be used for identifying the grain nickel ion accumulation QTL qNi.hnaas-6BS. It is identified that a quantitative trait locus (Quantitative Trait Locus, QTL) qNi.hnaas-6BS for controlling accumulation of nickel ions in wheat grains exists on a short arm of a wheat 6B chromosome, the QTL qNi.hnaas-6BS is closely linked with SNP6872 and is located at 125th, 482nd and 475th nucleotides of the 6B chromosome, and the SNP6872 has C / T polymorphism. A haplotype analysis result on a population level shows that when nucleotide at the site is CC, the wheat grains have relatively low nickel ion content, and are favorable allelic genotypes for limiting nickel ion accumulation of the wheat grains.

The invention discloses a protein OsEOG1 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed size regulation and is derived from the sequence 1. The OsEOG1 gene disclosed by the invention can control important yield traits of grain size in rice, that is, homozygous mutation of the gene causes increase of grain length and grain width of rice seeds and increase of hundred-grain weight. The invention provides a new gene resource for research on yield traits of plants, especially rice, selects a rice material with the excellent OsEOG1 allelotype for genetic improvement of rice, and plays an important role in application in the field of rice breeding.

This invention discloses a CAPS molecular marker for enhancing the cold tolerance gene OsSGR in rice, along with its detection and identification methods and applications, belonging to the field of plant molecular breeding technology. The molecular marker consists of two primers, OsSGR-CAPS-F (SEQ ID NO.1) and OsSGR-CAPS-R (SEQ ID NO.2), and can rapidly and accurately distinguish between two OsSGR alleles through PCR amplification, Nhe I restriction enzyme digestion, and electrophoretic analysis. The presence of only a 1038 bp band indicates a cold-sensitive genotype, while the presence of two bands (231 bp and 800 bp) indicates a cold-tolerant genotype. This invention also provides a method for genotype detection using this marker and its application in backcross breeding for selecting cold-tolerant rice lines. This marker system has advantages such as accurate detection, simple operation, and high breeding efficiency, making it suitable for large-scale application.

The invention relates to the technical field of modern livestock breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker rs637601961 related to the body weight of a goat at the age of 6 months and an application of the SNP molecular marker rs637601961. The SNP molecular marker is located at the 51bp position of a nucleotide sequence shown in SEQ ID NO.01, and the polymorphism of the SNP molecular marker is G / T. When the genotype of the polymorphic site contained in the molecular marker is GT or TT, the genotype corresponds to the relative advantage of the 6-month-old body weight of the goat to be detected. The molecular marker provided by the invention can be applied to the identification or prediction of the growth state of the goat, can also be applied to the field of molecular breeding, and judges the genetic potential of the goat based on the analysis of the genotype of the goat; by optimizing the dominant allelotype of the SNP molecular marker, the 6-month-old weight of the goat offspring can be improved, so that the SNP molecular marker is further used for genetic improvement of breeding sheep, and the economic benefit of the mutton sheep breeding industry is effectively improved.

The invention discloses a primer probe combination for detecting four genetic defect genes of a dairy cow and application of the primer probe combination. The primer probe combination for detecting citrullinemia, bovine spinal malformation syndrome, bovine leukocyte adhesion deficiency and / or bovine uridine monophosphate synthase deficiency of dairy cows can directly detect nucleic acid, and is high in accuracy, high in sensitivity, good in specificity, short in detection time and high in sensitivity. Allelic genotypes of related genes of four genetic diseases of the dairy cow can be rapidly, accurately and efficiently detected. Meanwhile, the primer probes are combined to prepare a detection kit for four disease-related gene mutation sites, so that powerful help is provided for accurately screening citrullinemia, bovine spinal malformation syndrome, bovine leukocyte adhesion deficiency and bovine uridine monophosphate synthase deficiency carriers of the dairy cow, and high-quality breeding of the dairy cow can be effectively assisted.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with salt-tolerant QTL-qGRNS6.01 / qRGR6.01 in a corn germination period and application of the molecular marker in breeding. A new major QTLqGRNS6.01 / qRGR6.01 for controlling the salt tolerance in the corn germination period is identified in multiple environments by utilizing a CSSL population and chip technology through linkage analysis, and KASP molecular markers are developed on SNP (Single Nucleotide Polymorphism) on two sides of QTL and can be used for molecular marker-assisted selective breeding. The qGRNS6.01 breeding effect evaluation result shows that the excellent allelotype from the NILB73 can obviously improve the germination rate character under the salt stress condition, so that the salt-tolerant effect is achieved.

The invention discloses a protein TIP1 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant grain size regulation and is derived from the sequence 1. The TIP1 gene disclosed by the invention can control the grain size character in the rice, namely the homozygous mutation of the gene can improve the grain size of the rice. According to the invention, a new gene resource is provided for research on grain size characters of plants, especially rice, and a rice material with excellent TIP1 allelotype is selected for genetic improvement of rice and plays an important role in application in the field of rice breeding.

The invention discloses an identification method for meiosis recombination of a citrus F1-generation hybrid population based on next-generation sequencing data. Sequencing the parents and the offspring; screening identification type SNP sites which are homozygous in one parent and heterozygous in the other parent; distinguishing the parent source and the parent source recombinant identification type SNP sites; in combination with the control parent, inferring an allelotype of the filial generation obtained from the identification parent and forming a filial generation chromosome haplotype; clustering the chromosome haplotypes of the offspring; constructing an identification parent fitting chromosome haplotype according to a majority principle; and comparing the fitting chromosome haplotype of the identified parent with the chromosome haplotype of the offspring to judge a recombination event (when the recombination of the male parent is analyzed, the male parent is the identified parent, and the female parent is the contrast parent, and the female parent is in the same way). The method provided by the invention breaks through the difficulty that the existing recombination analysis technology cannot identify the citrus hybrid population with few generations, high parent heterozygosity and unknown haplotype, so that the recombination identification of the perennial woody fruit tree population mainly comprising citrus becomes possible.

The invention belongs to the technical field of molecular genetics and molecular breeding, and relates to a molecular marker linked with a major QTL site related to the number of kernels per pod of peanut, a primer composition, an identification method and application, the major QTL site of the number of kernels per pod of peanut is located at the 05 chromosome of a peanut genome, and the molecular marker is InDel markers CNJ-9 and CNJ-12, a nucleotide sequence in a 600bp range in the Huayu No. 25 corresponding to the marker CNJ-9 is shown as SEQ ID NO: 1, and a nucleotide sequence in 600bp in the corresponding three-seed black is shown as SEQ ID NO: 2; a nucleotide sequence in a 600bp range in Huayu No. 25 corresponding to the marker CNJ-12 is as shown in SEQ ID NO: 4, and a nucleotide sequence in a 600bp range in corresponding three-seed black is as shown in SEQ ID NO: 5. Based on the developed markers CNJ-9 and CNJ-12, the allelotype of an individual at a target site can be accurately judged, and early, efficient and accurate selection of a target character is realized.

The application discloses a method for identifying meiotic recombination of citrus F1 hybrid population based on second-generation sequencing data. The parent and offspring are sequenced; the differential SNP sites which are homozygous in one parent and heterozygous in the other parent are screened; the paternal and maternal origin recombination differential SNP sites are distinguished; the control parent is combined to deduce the allele genotype of the offspring from the identification parent and to form the offspring chromosome haplotype; the offspring chromosome haplotype is clustered; the identification parent fitting chromosome haplotype is constructed according to the majority principle; and the identification parent fitting chromosome haplotype is compared with the offspring chromosome haplotype to determine the recombination event (when the father is analyzed, the father is the identification parent and the mother is the control parent; the mother is the same). The method breaks through the difficulty that the existing recombination analysis technology cannot identify the citrus hybrid population with less generation number, high parent heterozygosity and unknown haplotype, and makes it possible to identify the recombination of the perennial woody fruit tree population mainly with citrus.

The application discloses a SNP site related to the thousand kernel weight trait of Brassica napus and a KASP marker primer set thereof, belongs to the field of genetic engineering, and the SNP marker is located at the position of 3967014bp of the A09 chromosome of Brassica napus, the reference genome version is ZS11.v0, has G / A polymorphism, A is an excellent allelic genotype, the phenotype contribution rate of the thousand kernel weight is 10.0%-12.8%, and the individual with the genotype AA or AG at the position has a higher thousand kernel weight trait than the individual with the genotype GG. A specific KASP marker primer set is developed for the SNP site, the KASP marker primer set can realize the site genotyping, can be used for molecular marker assisted breeding selection, accelerates the identification and breeding of the thousand kernel weight trait in the rapeseed breeding process, and improves the breeding screening efficiency.

The invention belongs to the technical field of molecular genetic breeding, and particularly relates to a KASP molecular marker related to the protein content of rice grains and application of the KASP molecular marker. The invention provides a molecular marker developed on the basis of a KASP technology and used for detecting the protein content character of rice grains. The molecular marker comprises KASP-OsGluA2 and KASP-OsAAP6, wherein the molecular marker comprises KASP-OsGluA2 and KASP-OsAAP6; the KASP-OsGluA2 is located at the 31st basic group of the nucleotide sequence as shown in SEQ ID NO: 1, and has T / A polymorphism; the KASP-OsAAP6 is located at the 40th site of the nucleotide sequence as shown in SEQ ID NO: 6, and has C / CTCTGTG polymorphism. The molecular marker disclosed by the invention can be used for rapidly and accurately identifying the allelotype of the OsGluA2 and the OsAAP6 in a high-throughput manner, and has important significance on improving the protein content of rice grains so as to cultivate high-quality rice varieties.

The invention discloses a protein OsGSK3 related to rice grain size regulation and control as well as a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed size regulation and is derived from the sequence 1. The OsGSK3 gene disclosed by the invention can control important yield traits of grain size in rice, that is, homozygous mutation of the gene leads to increase of grain length and grain width of rice seeds and increase of hundred-grain weight. The invention provides a new gene resource for research on yield traits of plants, especially rice, selects a rice material with the excellent OsGSK3 allele for genetic improvement of rice, and plays an important role in application in the field of rice breeding.

The present invention discloses an SNP molecular marker for identifying the haplotype of the rice fertility restoration gene Rf4 and its application in restoring rice fertility. The present invention discovers that there are copy number variations and sequence base variations in the rice wild abortive cytoplasmic male sterility fertility restoration gene Rf4, and there are a total of 7 allelic genotype sequences and 8 haplotypes, including 5 single-copy haplotypes: rf4j, rf4i, rf4aus, Rf4a<supgt;I< / supgt;, and Rf4b<supgt;M< / supgt>, as well as 3 double-copy haplotypes: Rf4a<supgt;I< / supgt>-rf4b, rf4a-Rf4b<supgt;M< / supgt>, and Rf4a<supgt;M< / supgt>-Rf4b<supgt;M< / supgt>. Based on these allelic genotype sequences and SNP variations, the present invention develops a set of molecular markers to achieve the purpose of screening and identifying rice varieties, screening strong restoring rice containing the double-copy haplotype of Rf4a<supgt;M< / supgt>-Rf4b<supgt;M< / supgt>, and accelerating the breeding and identification of strong restoring lines by crossing with sterile lines.