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95 results about "Allelotype" patented technology
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Allelotype describes the occurrence of an allele in a population. Specifically, it describes the frequency distribution of a given set of alleles in a population. Allelotype is important for the field of population genetics, particularly when studying complex or multifactorial disorders such as cancer. Determining tumor allelotypes increases the understanding of the underlying tumorigenesis and improves the prognosis in tumor patients.
Annotation method and annotation system of whole-genome variant data
InactiveCN106156538AAccurate NotesSolve the screening puzzleProteomicsGenomicsReference genesAllele frequency
The invention discloses an annotation method and an annotation system of whole-genome variant data. The method comprises the following steps of S1, creating a variant data file, wherein the variant data are stored according to a national standard VCF format as an input file; S2, performing multi-allele genotyping, firstly performing genotype judgment, representing a basic group which is consistent with a reference genome by zero, and representing the basic groups which are inconsistent with the reference gene group by 1, 2, 3,..., then performing SNP and InDel multi-allele type resolution so that the allele type is represented by zero and one; S3, causing InDel generation position normalization, namely performing InDel generation position normalization according to a left justification and simplification normalization method; and S4, performing annotation, namely performing gene structure annotation, allele frequency annotation, variable site harm prediction and pathogenicity annotation. The annotation method and the annotation system improve integrity and accuracy of annotation information.
Owner:天津诺禾医学检验所有限公司
Method and system for implementing typing based on polymerase chain reaction sequencing
ActiveCN101984445AProcessing speedImprove typing efficiencySequence analysisSpecial data processing applicationsTypingAllelotype
The invention discloses a method and system for polymerase chain reaction sequencing-based typing (PCR-SBT). The method comprises the following steps: a heterozygote site and a base sequence to be typed are interpreted through a computer program according to a sequencing result; the base sequence to be typed is compared to a typing database of a corresponding site, an alignment position relation between the base sequence to be typed and reference sequences in the typing database is recognized; an allelic genetype in the typing database is searched, a penalty score of the allelic genetype in the typing database is obtained according to a sequencing strategy; and a candidate type group set is obtained according to the penalty score of the allelic gene type in the typing database. In the SBT method and system provided by the invention, automatic recognition of candidate genetypes can be achieved through a computer and other devices, thereby improving typing efficiency; and the convenience is provided for confirmation and modification of typing of typing personnel through imaging interface display and other technical means, thus typing accuracy and typing efficiency are provided.
Owner:BGI SHENZHEN CO LTD
Methods for PCR and HLA typing using raw blood
Owner:GENOMICS USA
PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3
ActiveCN103882145AResolving Poor Marker PolymorphismResolve accuracyMicrobiological testing/measurementAgricultural scienceElectrophoresis
The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Microsatellite instability prediction method and application thereof
ActiveCN111304303AImprove performanceImprove accuracyMicrobiological testing/measurementBiostatisticsGeneticsScreening method
The invention discloses a microsatellite instability detection and prediction method, a related site screening method, a model construction method and an application. The site screening method comprises the following steps: i) determining a microsatellite instability predicted value of a candidate microsatellite site in a sample; and / or ii) screening sites or site combinations for microsatellite instability detection by using one or more indicators including the microsatellite instability predicted value of the candidate microsatellite site; wherein the microsatellite instability predicted value is a quantified value of an abundance difference between a major allele type and a minor allele type in the candidate microsatellite site. The method provided by the invention is high in accuracy,and can accurately and stably detect the MSI state only on the basis of a single tumor sample.
Owner:BERRY ONCOLOGY CO LTD
Detection method and kit of HLA (Human Leukocyte Antigen)-B*58:01 allele
The invention relates to a detection method of HLA (Human Leukocyte Antigen)-B*58:01 allele. The detection method comprises the following steps: providing a mixture of a sample to be tested, a nucleic acid amplification system and a fluorescence detection system; circularly amplifying target polynucleotide through an amplified reaction; indirectly combining a fluorescence generating group and an amplified target polynucleotide sequence; and detecting the fluorescent amount generated by the fluorescence generating group so as to determine existence of the target polynucleotide and the relative amount thereof. The invention further discloses a kit for detecting the allele. The kit comprises a plurality of sealed centrifuge tubes which are respectively filled with a reaction liquid 1, a reaction liquid 2, a reaction liquid 3, an EZ Taq enzyme mixed liquid, a standard liquid 1 and a standard liquid 2 as well as the kit which separates and packages the centrifuge tubes in a centralized manner. The kit and the detection method provided by the invention are simple and convenient to operate, short in time consumed, strong in specificity and high in sensitivity. The kit and the detection device can be widely applied to detecting the HLA-B*58:01 allele and clinically avoiding severe untoward effects of skins of the HLA-B*58:01 allele patients caused by using allopurinol.
Owner:GUANGDONG UNITY BIOTECH
SNP marker affecting yean trait of Huyang sheep (sheep raised in Zhejiang Province) and application of SNP marker
ActiveCN108410994AIncrease lamb sizeSpeed up the breeding processMicrobiological testing/measurementDNA/RNA fragmentationEconomic benefitsAllele
The invention belongs to the fields of molecular biotechnology and molecular marker technology, and relates to applications of an SNP molecular marker in researches on yean trait of sheep and in sheepbreeding. The site of the sheep SNP molecular marker is shown as SEQ ID NO.1, and nucleic acid single-base mutation at the 193rd site of the sequence fragment is named as G765A. The SNP molecular marker corresponds to G>A mutation at the 765bp of a coding region sequence of a BMPR-IB gene (accession number: NC-019463.2) on the No.6 sheep chromosome, which is issued, on GeneBank. According to theinvention, by optimizing a dominant allele type of the SNP molecular marker, a genetic progress of yean trait of the Huyang sheep can be accelerated and breeding time of reproduction trait of the Huyang sheep can be shortened, so that reproduction cost is reduced, and economic benefits of mutton sheep breeding industry are effectively improved.
Owner:SOUTH CHINA AGRI UNIV
Cultivation method and application of recessive white feather new strain of Xiaoxiang chicken in southeast of Guizhou province
ActiveCN104823919AIncrease gene homozygosityHigh genetic stabilityAnimal husbandryMarker-assisted selectionF1 generation
The invention discloses a cultivation method and application of a recessive white feather new strain of Xiaoxiang chicken in southeast of Guizhou province. The method includes the following steps: (1) selfcrossing breeding is carried out on white feather chicken separated from the Xiaoxiang chicken in southeast of Guizhou province to obtain a F1 generation; (2) according to molecule marker-assisted selection, individuals of recessive homozygosis allelic genotypes of II are screened from the F1 generation; (3) test mating between a sanhuang chicken pure line or other colored feather chicken pure lines and the F1 is carried out, the dominance white feather individual and the colored feather individual which are separated from the F1 are rejected, recessive pure individual cock and hen are bred in cooperation with detection results in the step (2), and then self-reproducing is carried out to obtain B; according to the method in the step (3), repeated test mating and breeding are carried out on W in the step (3), and the separated dominance white feather individual and the separated colored feather individual are rejected continuously; selfcrossing breeding and group extending are carried out on the selected dominance white feather individual, a family is formed, and the recessive white feather new strain is cultivated.
Owner:GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
Susceptible/resistant MUC13 molecular marker capable of identifying F4 weaning brash of piglets and application
InactiveCN101532052AReduce mortalityImprove resistance to diarrheal diseaseMicrobiological testing/measurementAgricultural scienceMarker-assisted selection
The invention discloses a susceptible / resistant MUC 13 molecular marker capable of identifying F4 weaning brash of piglets of western pig breeds and application thereof to genetic improvement of pig breeds. The molecular marker firstly identifies single nucleotide polymorphism (SNP) A157G, and then adopts a SNaPshot method to perform genotype judgment on the SNPA157G. The invention detects a polymorphic site through modern molecular biology technology, utilizes the marker assisted selection (MAS) to select advantageous allelotype reserved individual breed, can obviously improve weaning brash resistance of species groups, and greatly reduces the death rate of the piglets.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
Molecular marker and method for identifying allelotype of rice chalk gene Chalk5
InactiveCN105925689AAccurately reflectAccurate operationMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceAllele
The invention relates to a molecular marker for identifying an allelotype of a rice chalk gene Chalk5. Two pairs of specific PCR (polymerase chain reaction) primer molecular markers, namely Chalk12 and NChalk12 as well as Chalk22 and NChalk22, are developed in accordance with functional mutant sites of dominant and recessive alleles of a major gene Chalk5 which is capable of significantly relieving rice chalk so as to improve the appearance quality of rice. In addition, the invention relates to a method for identifying the allelotype of the rice chalk gene Chalk5, wherein DNAs of various rice plants are amplified by virtue of the molecular marker. The molecular marker and the method disclosed by the invention not only can accurately identify whether the rice contains the rice chalk gene Chalk5 or not but also can further distinguish homozygote and heterozygote of the Chalk5 gene, so that the selection efficiency of rice grain weight is improved.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Clone and application of pleiotropic gene Ghd8 for controlling yield, florescence and plant height of rice grain
InactiveCN101892246AAvoid time-consuming disadvantagesSimple methodPlant peptidesGenetic engineeringGenotypeCorrelation analysis
Owner:HUAZHONG AGRI UNIV
BMP6 gene as molecular marker of litter size trait of black goat
ActiveCN108531608AIncrease lamb sizeSpeed up the breeding processMicrobiological testing/measurementDNA/RNA fragmentationLitterGenotype
The invention belongs to technical fields of molecular biology technology and molecular markers, and specifically relates to a BMP6 gene as a molecular marker of a litter size trait of a black goat. An SNP molecular marker site of the goat is as shown in SEQ ID NO.1, the 179th nucleic acid of the sequence fragment undergoes single base mutation and is named T951C. The SNP molecular marker is corresponding to T greater than C mutation at the 951bp (calculated with the first base of a coding zone sequence as +1 site) site in the coding zone sequence of the BMP6 gene (accession number: AC_000180.1) on No.23 chromosome of bovine released in GeneBank. Further analysis finds that the mutation fails to cause the coded amino acid sequence to change and represents synonymous mutation. A dominant allele genotype of the SNP molecular marker is selected, thereby accelerating genetic progress of the litter size trait of the goat, shortening breeding time of a reproductive trait of the goat, lowering reproduction cost, and increasing economic profits of meat goat breeding industry effectively.
Owner:SOUTH CHINA AGRI UNIV
Genetic testing for risk of causing serious adverse reactions of skin of carbamazepine
InactiveCN102108382AMicrobiological testing/measurementFluorescence/phosphorescenceAdverse drug reactionAntiepileptic drug
The invention discloses a genetic testing method for risk of causing serious adverse reactions of carbamazepine. The genetic testing method comprises the following steps: collecting oral mucosa cells of a subject, extracting genome DNA (deoxyribonucleic acid) of the oral mucosa cells, testing HLA (human leukocyte antigen)-B*1502 allelotype of the genome DNA and evaluating the risk of causing the serious adverse reactions of the skin of an anti-epileptic medicament-carbamazepine, thereby providing reference basis for clinical individual medication.
Owner:XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
Methods for PCR and HLA typing using raw blood
Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the secondary PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample, hybridizing the resulting amplicon or sets thereof to probes with sequences of gene-associated allele variations. A detectable signal indicating hybridization corresponds to an allelotype of the gene or a set of allelotypes for the set of genes.
Owner:GENOMICS USA
Method for qualitatively detecting HLA-B*1502 gene with PCR-SSP method and clinical kit
ActiveCN103114138ALow costLow instrument requirementsMicrobiological testing/measurementCarbamazepineMedication use
The invention belongs to the field of biotechnology, and particularly relates to a method for qualitatively detecting HLA-B*1502 gene with a PCR-SSP method and a clinical detection kit. The method comprises the following steps of: finding 6 specific areas capable of effectively identifying HLA-B*1502 allele type; designing 6 specific primers covering the 6 specific areas, and screening out a high-specificity primer pair applicable to PCR-SSP; and adding dUTP and UDG enzyme (uracil-DNA glycosylase) into a reaction system to solve the problem of PCR cross pollution and further improve the detection reliability. An HLA-B*1502 quick and convenient qualitative detection kit is researched and developed accordingly. The method and the clinical detection kit provided by the invention have the advantages of convenience in operation, short time, strong specificity, high accuracy, low cost and the like, and is suitable for realizing individual safe and reasonable drug use through HLA-B*1502 genotype detection before the Chinese or Asian epileptics take carbamazepine and phenytoin sodium.
Owner:GRACELL BIOTECH SHANGHAI CO LTD
KASP molecular marker of wheat powdery mildew resistance gene Pm21 derived from haynaldia villosa and application of KASP molecular marker
ActiveCN111235295AImprove transgenic utilizationQuick checkMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The present invention discloses a KASP molecular marker of a wheat powdery mildew resistance gene Pm21 derived from haynaldia villosa and an application of the KASP molecular marker. The KASP molecular marker is KASP-Pm21 and primers of the KASP molecular marker KASP-Pm21 comprise a haynaldia villosa allelotype upstream primer KASP-Pm21-F, a wheat allelotype upstream primer KASP-Pm21-H and a shared downstream primer KASP-Pm21-C; a nucleotide sequence of the haynaldia villosa allelotype upstream primer KASP-Pm21-F is shown in SEQ ID NO:1; a nucleotide sequence of the wheat allelotype upstream primer KASP-Pm21-H is shown in SEQ ID NO:2; and a nucleoside sequence of the shared downstream primer KASP-Pm21-C is shown in SEQ ID NO:3. The provided KASP molecular marker KASP-Pm21 of the wheat powdery mildew resistance gene Pm21 derived from the haynaldia villosa can realize efficient tracking and detection of the Pm21 gene and is applied to molecular marker assisted selection breeding of the wheat powdery mildew resistance Pm21 gene.
Owner:YANTAI UNIV
Molecular marker for identifying resistance traits of Phyricularia oryzae, identification method and application
InactiveCN108018371AEasy to distinguishImprove detection efficiencyMicrobiological testing/measurementDNA/RNA fragmentationEnzyme digestionAgricultural science
The invention belongs to the technical field of molecular biology and discloses a molecular marker for identifying resistance traits of Phyricularia oryzae, an identification method and an application. The molecular marker is Pib-TPAP and contains Pib-TPAP-O-F with a nucleotide sequence represented as SEQ ID NO.1, Pib-TPAP-O-R with a nucleotide sequence represented as SEQ ID NO.2, Pib-TPAP-I-F with a nucleotide sequence represented as SEQ ID NO.3 and Pib-TPAP-I-R with a nucleotide sequence represented as SEQ ID NO.4. The molecular marker can be used for directly identifying two allelotypes ofMagnaporthe oryzae resistance of a Pib gene, on the basis of a PCR (polymerase chain reaction) technology, the two disease-resistant allelotypes can be accurately identified by one-time PCR without sequencing or enzyme digestion, so that detection efficiency of a genetic locus is greatly improved, and accuracy rate reaches 100%.
Owner:LIAONING PROVINCIAL SALINE ALKALI LAND UTILIZATION & RES INST
KASP functional molecular marker of rice blast resistance gene Pi2 and application of KASP functional molecular marker
InactiveCN109385466AGenetic improvementImprove breeding efficiencyMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceAllelotype
The invention discloses a KASP functional molecular marker of a rice blast resistance gene Pi2 and an application of the KASP functional molecular marker, and relates to the technical field of rice breeding. The KASP functional molecular marker is marked as variant GCA GGA / GTG TTA on codons in 787-poisition and 788-poisition of the gene Pi2. With the adoption of the KASP functional molecular marker as well as primers and a kit thereof, an allelotype of the rice blast resistance gene Pi2 of a breeding material can be detected in the early stage (a seed or seedling stage), rice blast resistanceof the breeding material is predicted, and the breeding material is accurately screened and is not required to be planted in a disease nursery for evaluation. Therefore, genetic improvement of a ricevariety with rice blast resistance is promoted, and efficiency of variety breeding is improved. An SNP (single nucleotide polymorphism) site is detected with a KASP method, the detection method is simple to operate and low in cost, a detection result is accurate and reliable, and the KASP functional molecular marker is environmentally friendly, harmless to a human body and suitable for high-throughput large-scale commercial breeding application.
Owner:RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof
InactiveCN1680599AReduce mortalityMicrobiological testing/measurementAnimal husbandryEnzyme GeneMarker-assisted selection
A single nucleotide polymorphic locus of a1,2- fucose transfer enzyme gene affecting obviously resistant dropsy and diarrhea. It identifies single nucleotide polymorphic locus(SNP) c228T firstly and then judges type of SNPC 228T gene by way of PCR-SS CP,ARMS-PCR and so on.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
A PCR molecular marker method for identifying allelic variation of rice grain length gene qgl3
ActiveCN103882145BRapid identificationImprove appearance qualityMicrobiological testing/measurementAgricultural scienceGermplasm
The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.
Owner:JIANGSU ACAD OF AGRI SCI
Kit for detecting warfarin sensitivity gene by using electrochemical gene sensor method
InactiveCN103627789AHigh sensitivityStrong specificityMicrobiological testing/measurementHuman DNA sequencingWarfarin
The invention provides a warfarin sensitivity gene detection kit (an electrochemical gene sensor method) which is a kit used for rapid detection of a warfarin sensitivity gene in a human genome by using electrochemical gene sensor technology. The kit is applicable to qualitative detection of *2, *3 and *5 allele types of the cytochrome CYP2C9 gene and 1639G>A and 1173C>T allele types of vitamin K epoxide reductase complex 1 (VKORC 1) in clinical specimens like human peripheral blood and a variety of tissue and cells; the above-mentioned genotypes are helpful for clinical discrimination of patients with increased drug susceptibility to warfarin and enable the dosage range of warfarin to be rapidly determined, curative effects to be guaranteed and the risk of hemorrhage to be reduced. Combination of warfarin gene detection results with INR monitoring enables the maintenance dose of warfarin to be effectively and rapidly adjusted, thereby lowering down the risk of hemorrhage while achieving curative effects.
Owner:DAAN GENE CO LTD
HLA typing method and equipment based on Sanger sequencing
PendingCN112669903ASolve the speed problemResolve accuracyProteomicsGenomicsTyping methodsNucleic acid sequencing
The invention relates to an HLA typing method and equipment based on Sanger sequencing. The HLA typing method comprises the following steps: acquiring sequencing data of a gene to be identified; according to the sequencing data of the to-be-identified gene, based on a pre-trained base identification model, obtaining a correct base signal of each site of the to-be-identified gene; and interpreting a nucleic acid sequence of the to-be-identified gene, comparing the nucleic acid sequence with a reference sequence to obtain a difference site, and comparing by adopting bit operation based on a pre-arranged allele type and the difference site to obtain an HLA typing result. Because the base recognition model is trained by taking sequencing data of abnormal bases as sample data, the base recognition model obtained by training can recognize abnormal bases such as low-quality heterozygous bases and pollution signals in Sanger sequencing data, and an allele typing result is quickly obtained by using bit operation; and the problems of low speed and low accuracy of manual recognition are solved.
Owner:北京旌准医疗科技有限公司
Sheep UCP1 (uncoupling protein 1) allelotype detection method and detection kit
InactiveCN105925701AEasy to detectQuick checkMicrobiological testing/measurementAnimal scienceUcp1 gene
The invention provides a sheep UCP1 (uncoupling protein 1) allelotype detection kit which comprises a specific primer pair and sheep UCP1 allelotype standard DNAs, wherein the specific primer pair comprises a sense primer 5'-AGATACAAGCGGAAGAGACAC-3' and an antisense primer 5'-TGAAGGGTTGGGTCTGTCA-3'; the sheep UCP1 allelotype standard DNAs are SEQ ID No. 1-3 in a sequence table. The invention further provides a sheep UCP1 allelotype detection method. By the detection method, different sheep UCP1 allelotypes and sheep carcass fat content characters are associatively analyzed, and researches show that a sheep UCP1 allelotype has remarkable influence (P<0.05) on a carcass fat content character of a sheep population. Therefore, a sheep UCP1 gene can be determined to improve the sheep carcass fat content character as a molecular marker.
Owner:COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
SNP molecular marker related with pig growth velocity and application thereof
ActiveCN109811063ASpeed up breeding progressFast growthMicrobiological testing/measurementDNA/RNA fragmentationComplete linkageGenotype
The invention provides an SNP molecular marker related with pig growth velocity and application thereof. According to the SNP molecular marker disclosed by the invention, a TRPC1 gene is utilized as acandidate gene which can affect the pig growth velocity; a sequencing technique is utilized to screen and identify SNP loci related with the pig growth velocity to obtain 2 SNP loci which can affectthe pig growth velocity and a gene type effect; the 2 SNP loci are respectively arranged (595bp)th and (539bp)th positions of a sequence shown in SEQ ID NO.1; allelotype of the 2 SNP loci is C and T;furthermore, alleles of C and C and T and T of the 2 SNP loci are completely linked on a genome; thus, any SNP locus can be applied to breeding pig breed growth rate characters; furthermore, the SNP loci cannot be limited by ages, genders and breeds of pigs, can be applied to early breeding of the pigs and can quicken a breeding process.
Owner:CHINA AGRI UNIV
Molecular markers for identifying PHYB wild type and mutant of rice phytochrome gene
ActiveCN106282345AValid identificationRapid identificationMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The invention discloses molecular markers for identifying the PHYB wild type and mutant of a rice phytochrome gene. The nucleotide sequences of the markers related to phyB wild type site detection and phyB mutant site detection are shown as Seq No.1-2 respectively. A primer PHYBF4 / PHYBR1 is used for carrying out PCR amplification, and an amplification product is subjected to BamH I digestion and electrophoretic analysis, wherein if the PCR product can not be digested by BamH I and is a 175bp DNA fragment, the PCR product is the wild type phyB, and if the PCR product can be digested by BamH I, and the digestion products are a 25bp DNA fragment and a 151bp DNA fragment, the PCR product is the mutant phyB1. The molecular markers have the advantages that amplification is easy and fast, and cost is low. The molecular markers can be used for effectively, rapidly and reliably identifying the phyB allelotype, and a powerful tool is provided for subsequent application of the phyB mutant in breeding population screening.
Owner:SHANDONG RICE RES INST
Molecular marker for identifying rice grain length characters, identification method and application
InactiveCN107245530AEasy to operateReduce consumptionMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceNucleotide
Owner:LIAONING PROVINCIAL SALINE ALKALI LAND UTILIZATION & RES INST
Flight mass spectrometry genotyping detection method
InactiveCN111041079AReduce dosageEasy to operateMicrobiological testing/measurementMaterial analysis by electric/magnetic meansEnzyme digestionAllele-specific oligonucleotide
The invention discloses a flight mass spectrometry genotyping detection method. The method comprises the following steps: (1) designing three oligonucleotide probes for a SNP site, wherein the 3 / endsof a first probe and a second probe are respectively two allelic genes, i.e., allele-specific oligos (ASOs) of a pre-selected SNP site, (2) when the sequence is completely matched with the target sequence, preparing a connection product, (4) carrying out enzyme digestion on the PCR product by using restriction endonuclease, and purifying the product by using streptavidin-coated beads, and (5) transferring the purified product to a 384-well chip. SNP site typing is carried out by adopting MALDI-TOF mass spectrometry, and SNP typing is provided by adopting the method which is simple to operate,low in cost and high in resolution ratio.
Owner:博淼生物科技(北京)有限公司
Molecular marker primer for rapidly and efficiently identifying rice salt tolerant gene SKC1 and application
InactiveCN111378781AAccurate identificationRapid identificationMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyElectrophoreses
The invention discloses a molecular marker primer for rapidly and efficiently identifying a rice salt tolerant gene SKC1 and an application. Specific to the specific difference sites present in the initially reported salt-tolerant (Nona-type) and salt-sensitive (sensitive type) SKC1 gene sequences, a molecular marker primer SKC1-Allele1 / Allele2 / Common for distinguishing the salt-tolerant gene type and sensitive type of the rice salt-resistant gene SKC1 is designed, thereby accurately, quickly and efficiently identifying the SKC1 allele, and providing a powerful tool for the subsequent cultivation of a new strain of salt tolerant rice. Compared with the prior art, the method is more rapid and time-saving, saves the time and effort of an agarose electrophoresis test, and a result can be obtained by only needing 30 seconds of fluorescence scanning after a PCR amplification procedure is finished, the result is clear at a glance, and the accuracy is improved.
Owner:SHANDONG RICE RES INST
System and method for inferring str allelic genotype from snps
The present invention provides methods to infer STR allelic genotype from SNPs in a genome by obtaining statistical probabilities for the association of a plurality of SNPs in a genome with a Short Tandem Repeat (STR) locus allele for the genome to obtain a SNP constellation association value.
Owner:GENOME ID GRP LLC
Functional marker of maize recessive nuclear male sterility mutant gene ms1 and applications thereof
The invention relates to a functional marker of a maize nuclear male sterility gene ms1, a functional marker developing method and applications of the functional marker. According to the mutation siteof the mutant gene ms1, the functional marker which comprises two functional markers ms1-IN1 and ms1-IN2 is designed to ensure that a maize mutant ms1-alb and the mutant gene ms1 in a maize sterile material transferred from mutant can be specifically detected; and by utilizing the ms1-IN1, the ms1-IN2 or other similar functional markers and functional markers Ms1-1, Ms1-2 or other similar functional markers of a corresponding wild type allele Ms1, the situation of a nuclear male sterility gene Ms1 allele in a sample can be quickly identified through PCR (Polymerase Chain Reaction) detection and agarose gel electrophoresis detection. By utilizing the functional marker designed by the invention, the allele genotypes of the maize nuclear male sterility gene ms1 or a fertile gene Ms1 can be accurately detected and marked; the functional marker can be used for identification of male sterile strains and screening of target single stains; and the functional marker has important application value in maize male sterile strain breeding, sterile hybrid seed production and molecular marker-assisted selection and has important significance for improving maize cross breeding efficiency and seedproduction efficiency, guaranteeing seed production quality and the like.
Owner:UNIV OF SCI & TECH BEIJING +1
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