16 results about "Allelotype" patented technology

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The application discloses a genetic marker linked with a wheat kernel nickel ion accumulation QTL qNi.hnaas-6BS, adopts a Wheat Breeders 660K microarray chip to identify genotype data of a natural population, combines phenotype data of kernel nickel ion content of the wheat natural population, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BS for controlling wheat kernel nickel ion accumulation exists on a short arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP6872, is located at the 125,482,475th nucleotide of the 6B chromosome, and the SNP6872 has C / T polymorphism. Haplotype analysis results on a population level show that when the nucleotide of the site is CC, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype for limiting the accumulation of the wheat kernel nickel ion.

The application discloses a molecular marker related to wheat kernel nickel ion accumulation, uses a natural population constructed by a team in the early stage, uses a Wheat Breeders 660K microarray chip to identify genotypic data of the natural population, combines phenotypic data of the wheat natural population kernel nickel ion content, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BL controlling the wheat kernel Ni ion accumulation exists on a long arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP4008, is located at the 693,584,519th nucleotide of the 6B chromosome, and the SNP4008 has A / G polymorphism. Haplotype analysis results on the population level show that when the nucleotide of the site is AA, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype; when the nucleotide of the site is GG, the wheat kernel has higher nickel ion content, and is an unfavorable allelic genotype.

The invention belongs to the field of rice molecular genetics, and particularly discloses an MBKbase database-based dominant allelotype analysis method for stem strength genes, which comprises the following steps: firstly, analyzing different genotypes of rice stem strength genes SCM3 and OsRLCK191, and according to different genotype germplasm resources of the rice stem strength genes carried in MBKbase-Rice, constructing a dominant allelotype of the rice stem strength genes SCM3 and OsRLCK191; the method comprises the following steps: selecting rice materials with single-dominant allelic genes, double-dominant allelic genes and non-dominant allelic genes (reference genome type), determining the stem strength character of rice germplasm resources of different genotype combinations of the rice stem strength gene, and finally performing sequencing verification on different genotypes of the rice stem strength gene. Finally, clearly breeding a strategy that the stalks are thick and strong, the stalks are high in elasticity or the stalks are moderate in thickness and elasticity to improve the lodging resistance of the rice variety; therefore, low-cost and high-throughput genotype screening can be realized.

The invention discloses a protein TaEOG1 related to wheat grain quality regulation and control as well as a coding gene and application of the protein TaEOG1. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed quality regulation and is derived from the sequence 1. The TaEOG1 gene disclosed by the invention can control grain quality characters in wheat, namely homozygous mutation of the gene causes increase of protein content and precipitation value of wheat seeds. A new gene resource is provided for research on quality characters of plants, especially wheat grains, a wheat material with the TaEOG1 excellent allele type is selected for genetic improvement of wheat, and the gene plays an important role in application in the field of wheat breeding.

The invention belongs to the technical field of plant genetic engineering, and discloses an application of a gene Gohi.A03G043500 in salt-tolerant breeding of cotton. The invention discloses the expression characteristic of stress-induced down-regulation of the gene in the salt-tolerant cotton and the key function of the gene as a stress negative regulation factor for regulating growth-stress resistance balance for the first time. The salt tolerance of the cotton germplasm can be accurately identified by detecting the expression change of the gene under salt stress or the allelotype of a regulation region of the gene; meanwhile, the salt tolerance of the cotton can be effectively improved by accurately regulating and controlling the expression level of the gene. The invention provides a new functional marker, a breeding strategy and a gene resource for salt-tolerant breeding of cotton, and has important application value in breeding of salt-tolerant cotton varieties.

The invention discloses a protein LBL2 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant grain size regulation and is derived from the sequence 1. The LBL2 gene disclosed by the invention can control the grain size character in rice, namely overexpression of the gene can increase the length and thousand grain weight of rice grains. According to the invention, a new gene resource is provided for research on the grain size character of plants, especially rice, and a rice material with an excellent LBL2 allele type is selected for genetic improvement of rice and plays an important role in application in the field of rice breeding.

The invention discloses a protein OsEOG1 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed size regulation and is derived from the sequence 1. The OsEOG1 gene disclosed by the invention can control important yield traits of grain size in rice, that is, homozygous mutation of the gene causes increase of grain length and grain width of rice seeds and increase of hundred-grain weight. The invention provides a new gene resource for research on yield traits of plants, especially rice, selects a rice material with the excellent OsEOG1 allelotype for genetic improvement of rice, and plays an important role in application in the field of rice breeding.

This invention discloses a CAPS molecular marker for enhancing the cold tolerance gene OsSGR in rice, along with its detection and identification methods and applications, belonging to the field of plant molecular breeding technology. The molecular marker consists of two primers, OsSGR-CAPS-F (SEQ ID NO.1) and OsSGR-CAPS-R (SEQ ID NO.2), and can rapidly and accurately distinguish between two OsSGR alleles through PCR amplification, Nhe I restriction enzyme digestion, and electrophoretic analysis. The presence of only a 1038 bp band indicates a cold-sensitive genotype, while the presence of two bands (231 bp and 800 bp) indicates a cold-tolerant genotype. This invention also provides a method for genotype detection using this marker and its application in backcross breeding for selecting cold-tolerant rice lines. This marker system has advantages such as accurate detection, simple operation, and high breeding efficiency, making it suitable for large-scale application.

The invention discloses a protein TIP1 related to rice grain size regulation and a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant grain size regulation and is derived from the sequence 1. The TIP1 gene disclosed by the invention can control the grain size character in the rice, namely the homozygous mutation of the gene can improve the grain size of the rice. According to the invention, a new gene resource is provided for research on grain size characters of plants, especially rice, and a rice material with excellent TIP1 allelotype is selected for genetic improvement of rice and plays an important role in application in the field of rice breeding.

The invention discloses an identification method for meiosis recombination of a citrus F1-generation hybrid population based on next-generation sequencing data. Sequencing the parents and the offspring; screening identification type SNP sites which are homozygous in one parent and heterozygous in the other parent; distinguishing the parent source and the parent source recombinant identification type SNP sites; in combination with the control parent, inferring an allelotype of the filial generation obtained from the identification parent and forming a filial generation chromosome haplotype; clustering the chromosome haplotypes of the offspring; constructing an identification parent fitting chromosome haplotype according to a majority principle; and comparing the fitting chromosome haplotype of the identified parent with the chromosome haplotype of the offspring to judge a recombination event (when the recombination of the male parent is analyzed, the male parent is the identified parent, and the female parent is the contrast parent, and the female parent is in the same way). The method provided by the invention breaks through the difficulty that the existing recombination analysis technology cannot identify the citrus hybrid population with few generations, high parent heterozygosity and unknown haplotype, so that the recombination identification of the perennial woody fruit tree population mainly comprising citrus becomes possible.

The invention belongs to the technical field of molecular genetics and molecular breeding, and relates to a molecular marker linked with a major QTL site related to the number of kernels per pod of peanut, a primer composition, an identification method and application, the major QTL site of the number of kernels per pod of peanut is located at the 05 chromosome of a peanut genome, and the molecular marker is InDel markers CNJ-9 and CNJ-12, a nucleotide sequence in a 600bp range in the Huayu No. 25 corresponding to the marker CNJ-9 is shown as SEQ ID NO: 1, and a nucleotide sequence in 600bp in the corresponding three-seed black is shown as SEQ ID NO: 2; a nucleotide sequence in a 600bp range in Huayu No. 25 corresponding to the marker CNJ-12 is as shown in SEQ ID NO: 4, and a nucleotide sequence in a 600bp range in corresponding three-seed black is as shown in SEQ ID NO: 5. Based on the developed markers CNJ-9 and CNJ-12, the allelotype of an individual at a target site can be accurately judged, and early, efficient and accurate selection of a target character is realized.

The application discloses a method for identifying meiotic recombination of citrus F1 hybrid population based on second-generation sequencing data. The parent and offspring are sequenced; the differential SNP sites which are homozygous in one parent and heterozygous in the other parent are screened; the paternal and maternal origin recombination differential SNP sites are distinguished; the control parent is combined to deduce the allele genotype of the offspring from the identification parent and to form the offspring chromosome haplotype; the offspring chromosome haplotype is clustered; the identification parent fitting chromosome haplotype is constructed according to the majority principle; and the identification parent fitting chromosome haplotype is compared with the offspring chromosome haplotype to determine the recombination event (when the father is analyzed, the father is the identification parent and the mother is the control parent; the mother is the same). The method breaks through the difficulty that the existing recombination analysis technology cannot identify the citrus hybrid population with less generation number, high parent heterozygosity and unknown haplotype, and makes it possible to identify the recombination of the perennial woody fruit tree population mainly with citrus.

The application discloses a SNP site related to the thousand kernel weight trait of Brassica napus and a KASP marker primer set thereof, belongs to the field of genetic engineering, and the SNP marker is located at the position of 3967014bp of the A09 chromosome of Brassica napus, the reference genome version is ZS11.v0, has G / A polymorphism, A is an excellent allelic genotype, the phenotype contribution rate of the thousand kernel weight is 10.0%-12.8%, and the individual with the genotype AA or AG at the position has a higher thousand kernel weight trait than the individual with the genotype GG. A specific KASP marker primer set is developed for the SNP site, the KASP marker primer set can realize the site genotyping, can be used for molecular marker assisted breeding selection, accelerates the identification and breeding of the thousand kernel weight trait in the rapeseed breeding process, and improves the breeding screening efficiency.

The invention discloses a protein OsGSK3 related to rice grain size regulation and control as well as a coding gene and application thereof. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed size regulation and is derived from the sequence 1. The OsGSK3 gene disclosed by the invention can control important yield traits of grain size in rice, that is, homozygous mutation of the gene leads to increase of grain length and grain width of rice seeds and increase of hundred-grain weight. The invention provides a new gene resource for research on yield traits of plants, especially rice, selects a rice material with the excellent OsGSK3 allele for genetic improvement of rice, and plays an important role in application in the field of rice breeding.

The invention discloses application of two GhUspA genes and alleles thereof in high-temperature-resistant germplasm breeding of upland cotton, and belongs to the technical field of plant molecular breeding. The invention discloses protein coding sequences of two extensive stress protein genes GhUspA-At and GhUspA-Dt participating in high-temperature stress response of upland cotton for the first time, and four high-temperature-resistant allelic genotypes created through gene editing, and also provides a primer and a detection method for detecting the allelic genotypes. The gene is derived from a high-temperature-resistant site qPV-A01-1 and a homologous site qPV-D01 of upland cotton, the allelotype of the gene is obtained through gene editing, the high-temperature resistance of cotton pollen can be remarkably improved, and other agronomic characters are not affected. The gene resource, the allelotype and the molecular marker provided by the invention can be used for molecular marker-assisted breeding, accelerating the germplasm innovation and breeding process of high-temperature-resistant upland cotton, and solving the problem of squaring and boll falling caused by high temperature in the Xinjiang cotton area.