119 results about "Cashmere goat" patented technology

The invention relates to the technical field of gene engineering, in particular to application of a detection reagent for a hair follicle development related gene of a down producing goat. According to the present invention, the Cashmere goat hair follicle development related gene is any one or more of lncRNA MSTRG.20890.1, chi-miR-24-3p and ADAMTS3, and the lncRNA MSTRG.20890.1 and the ADAMTS3 are competitively combined with the chi-miR-24-3p, such that the significant differential expression of the lncRNA MSTRG.20890.1 in the Cashmere goat secondary hair follicle morphogenesis process is found, and the results prove that the lncRNA MSTRG.20890.1 can regulate the proliferation and the migration of dermal fibroblasts through the competitive combination of the lncRNA MSTRG.20890.1 and the ADAMTS3, such that the growth of the Cashmere goat secondary hair follicle morphogenesis can be inhibited therefore, the formation of a dermal papilla structure is inhibited.

The invention provides a multi-nutrient balanced lick brick for grazing down producing goats as well as a preparation method and application thereof, and belongs to the technical field of animal feed development. The multi-nutrient balanced lick brick is prepared from the following raw materials in parts by weight: 20 to 100 parts of copper sulfate pentahydrate, 35 to 165 parts of zinc sulfate monohydrate, 0.1 to 1 part of sodium selenite, 54.5 to 270 parts of anhydrous ferrous sulfate, 10 to 40 parts of manganese sulfate monohydrate, 15 to 65 parts of 1 percent cobalt chloride, 0.2 to 2 parts of potassium iodide, 40 to 200 parts of DL-alpha-tocopheryl acetate, 0.2 to 2 parts of vitamin A acetate, 40 to 200 parts of calcium carbonate and 20 to 100 parts of adhesive. And 8855-9765 parts of sodium chloride. The proportion of various nutritional ingredients is reasonably adjusted, the requirements of the grazing cashmere goats for microelements and vitamins can be comprehensively met, then the cashmere density is increased, and the cashmere yield is increased. Meanwhile, the fineness of cashmere can be reduced, and the quality of cashmere is improved.

The invention provides a grazing-supplementary feeding combined regulation and control method for promoting sustainable and efficient production of fattening Alba cashmere goats. The method specifically comprises the steps that the goat breeding scale is determined; grouping the goats; determining a goat grazing regulation and control mode; and determining a goat supplementary feeding regulation mode. According to the method, the grazing area, the everyday grazing duration and the grazing and returning time points of the fattening Alba cashmere goats are optimized and regulated, so that the energy additionally consumed by the goats due to defending against cold and excessive migration is reduced, and the ecological negative effect caused by excessive trampling and forage grass ingestion of the goats is also reduced; and sustainable grazing utilization of the grassland can be realized.

The invention provides a method for efficiently preparing FGF5 homozygous knockout cashmere goats by combining a base editor with a somatic cell cloning technology. And an sgRNA site is designed. An sgRNA expression vector is constructed, the sgRNA expression vector and pCMV-AncBE4max are respectively transferred into fibroblasts of a down producing goat fetus, and the editing efficiency is analyzed. And optimizing an electrotransfection condition, and simultaneously transfecting the screened sgRNA expression vector and pCMV-AncBE4max into a down producing goat fetal fibroblast to obtain the FGF5 homozygous knockout cell strain. And preparing reconstructed embryos and FGF5 homozygous knockout cashmere goats. The average editing efficiency of the sgRNA site disclosed by the invention can reach 64.3 + / -8.1%. The average probability of FGF5 homozygous knockout cells is 69.0%. Wherein the homozygous probability is 58.6% on average. The lambing rate is 7%. The invention provides a theoretical basis and a research basis for cultivating high-yield and high-quality new varieties.

The invention discloses a molecular marker influencing nap traits of northwest Tibetan white nap goats and application of the molecular marker. The molecular marker comprises five genome road signs of genes CCSER1, RPS6KC1, KCNRG, KCNK9 and CLYBL which influence the villus traits of the northwest Tibetan white cashmere goats; comprising application in identification of northwest Tibetan white cashmere goats. According to the method, the nap character of the northwest Tibetan white cashmere goat is taken as a research object for the first time, and whole genome association analysis is carried out on the basis of a goat 70KSNP chip sequencing result and nap fineness, nap length, nap length and nap yield phenotype data. A plurality of candidate genes are screened from significant SNP loci associated with GWAS through gene annotation and GO and KEGG enrichment analysis, the significance of the significant loci and the candidate genes is verified by adopting an international sequencing gold standard-Sanger sequencing method, and finally it is determined that a plurality of genes significantly affect the nap traits of the northwest Tibetan white cashmere goats, so that a theoretical basis is laid for subsequent further research, and the method has a broad application prospect in the field of screening of the nap traits of the northwest Tibetan white cashmere goats. And the method has very important scientific significance for analyzing a genetic mechanism related to the villus character.

The invention belongs to the technical field of genetic breeding, and particularly relates to an SNP marker influencing a fine cashmere goat and application thereof.The SNP marker comprises a first marker and / or a second marker, and the first marker and / or the second marker influence the fine cashmere size of the cashmere goat; the first marker is located at the 48854986 position on the No.6 chromosome of the down producing goat genome and mutates from G to A, and the second marker is located at the 76025546 position on the No.14 chromosome of the down producing goat genome and mutates from T to C.

The invention discloses a construction method of a Csdc2 overexpression vector and an interference cell model, and belongs to the technical field of cell engineering and gene engineering. Csdc2 is found to be an important gene for regulating and controlling periodic development of hair follicles, and gene expression is acted by related hormones and participates in regulation and control of villus growth of down producing goats. The Csdc2 is taken as a research object, the Csdc2 gene is overexpressed through a gene engineering technology, and the Csdc2 in the dermal papilla cell of the down producing goat can be accurately overexpressed; the expression of the goat Csdc2 gene at the mRNA level can be effectively inhibited by acting the siRNA for inhibiting the expression of the goat Csdc2 gene on the dermal papilla cells of the down producing goat, and successful establishment of a Csdc2 overexpression vector and siRNA interference cell model is proved. The Csdc2 gene is helpful for researching the function of the Csdc2 gene, and has a good application value for researching the molecular mechanism of periodic growth of secondary hair follicles of down producing goats and regulating and controlling the growth of villus.

The invention belongs to the technical field of animal gene engineering, and particularly relates to a down producing goat lncRNA, an interference vector and application of the interference vector to regulation and control of down producing goat hair follicle development, the down producing goat lncRNA is lncRNA TCONS-00237214 and is used for regulating and controlling down producing goat hair follicle development; the nucleotide sequence of the lncRNA TCONS-00237214 is as shown in SEQ ID NO. 1 (sequence identifier number 1). According to the invention, the hair papilla cells are selected for subsequent experimental verification, and the cell cycle, cell proliferation and cell migration of the hair papilla cells are detected by constructing an interference lncRNA TCONS-00237214 stable transformation hair papilla cell line. Results of the invention show that melatonin can regulate and control expression of lncRNA TCONS-00237214, participate in cell cycle transformation, promote proliferation and migration of hair papilla cells and inhibit apoptosis of the hair papilla cells, thereby affecting generation and development of hair follicles.

The invention provides a method for detecting genes related to early growth traits of Inner Mongolia down producing goats based on copy number variation (CNV). The method comprises the following steps: acquiring whole genome re-sequencing data of 461 Inner Mongolia down producing goats; the method comprises the following steps: detecting autosomal CNV by using CNV native software, and screening out 26,003 non-redundant CNV which covers 1, 015.4 Mb (38.97%) of an autosomal genome; gWAS analysis is carried out on the basis of CNV, 11 CNVs obviously related to early growth traits (birth weight CSZ, weaning weight DRZ, daily gain ADG before weaning and 12-month-old weight ZWT) are identified, and two CNVs influence ADG and DRZ at the same time; seven key candidate genes (ZN845, SOX15, FGF11, GPS2, DVL2, SPRY4 and STAT2) are analyzed and identified through bioinformatics, and GO and KEGG function enrichment analysis is utilized to find that the genes participate in biological pathways such as cell proliferation, differentiation and protein phosphorylation. The invention provides a theoretical basis for molecular marker-assisted breeding of Inner Mongolia down producing goats, and has important scientific value and application prospect.

The invention belongs to the field of functional feeds, and discloses a preparation method of a coarse cereal feed for sheep, which comprises the following steps: crushing one or two coarse cereals of buckwheat, broom corn millet, millet and sorghum, and mixing the crushed coarse cereals with raw materials such as enzymolysis soybean meal and alfalfa meal; adding a compound bacteria solution containing lactobacillus plantarum, lactobacillus acidophilus and saccharomyces boulardii for anaerobic fermentation; the nano zinc oxide and the yucca extract are further strengthened to improve the oxidation resistance and ammonia reduction capacity of the feed; and finally, granulating and spraying a sodium alginate-chitosan coating. Through coarse cereal combination optimization, soybean meal enzymolysis pretreatment and composite flora fermentation synergistic effect, the feed protein utilization rate, the fiber degradation rate and the animal production performance are remarkably improved. Experiments show that the milk fat content of the saanen milk goats fed with the feed is increased by 15-23%, the daily gain of the Alba cashmere goats is increased by 25-35%, the feed conversion ratio is optimized to 11.2-12.3: 1, and compared with the prior art, the feed has outstanding nutrition balance and application pertinence.

The present invention is applicable to the field of biotechnology, and provides a molecular marker related to the cashmere diameter trait of the Albas cashmere goat and its application, wherein the molecular marker includes at least one or several combinations of 17 SNP sites and 5 InDel sites located on chromosome 16 screened based on whole genome association analysis, and by detecting the molecular marker, the cashmere diameter trait of the cashmere goat can be predicted quickly, at low cost and effectively, which is of great significance in reducing the cashmere diameter and improving the quality of the wool. By using the above molecular markers, through molecular marker-assisted breeding, individuals with favorable genotypes are retained, and individuals with unfavorable genotypes are eliminated, so as to reduce the cashmere diameter of the group, thereby improving the fineness of the wool, achieving the purpose of improving the quality of the wool, and meeting market demand.

The present invention relates to the field of genetic engineering technology, and specifically discloses the application of an lncRNA inhibitor in the preparation of a fibroblast proliferation promoter. The lncRNA is lncRNA MSTRG.12349.1, and the only active ingredient in the lncRNA inhibitor is the interfering sequence of the lncRNA, and the interfering sequence is lncRNA MSTRG.12349.1-sh1. The interfering sequence lncRNA MSTRG.12349.1-sh1 provided by the present invention effectively improves the proliferation ability of fibroblasts by increasing the proportion of cells in the S phase of the cell cycle, and promotes the occurrence and development of secondary hair follicles in the embryonic period of cashmere goats.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of a molecular marker related to the hair length character of a down producing goat. The molecular marker is located at 155, 344, 701-155, 346 and 600 positions on a No.1 chromosome of a goat genome, and the nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1. It is found that the wool length of an individual with a normal molecular marker area is larger than that of an individual with a deletion molecular marker area, and therefore the cashmere goat variety with the large wool length can be bred by detecting deletion mutation of the molecular marker on the chromosome 1 of the goat genome.

The invention belongs to the technical field of biotechnology and livestock breeding, and particularly relates to a detection method for YAP1 gene insertion or deletion markers of Shaanbei white cashmere goats and application of the detection method. The method comprises the following steps: by taking whole genome DNA (Deoxyribose Nucleic Acid) of a to-be-detected Shaanbei white cashmere goat as a template, carrying out PCR (Polymerase Chain Reaction) amplification on partial fragments of a 5'upstream regulation region of a YAP1 gene, then carrying out agarose gel electrophoresis, and realizing rapid and accurate detection on insertion or deletion polymorphism on a No.15 chromosome (NC030822.1: rs645491525, g.7590812175908122 and NC030822.1: rs653744801 and g.7589798575898001) of a specific site of the YAP1 gene according to an electrophoresis result. A correlation analysis result shows that the YAP1 gene is remarkably related to the growth traits of the northern Shaanxi white cashmere goat, and can be applied to goat molecular marker-assisted selective breeding, so that the establishment of an excellent goat genetic resource group is accelerated.

The present invention relates to the use of a composition comprising one or more proteins from cashmere goat milk as cosmetic, medicament and / or dietary supplement.

The invention relates to the technical field of veterinary diagnosis, discloses a down producing goat pneumonia early diagnosis system and an application method thereof, and aims to solve the problems of insufficient accuracy, timeliness, convenience and continuity in existing down producing goat pneumonia early diagnosis. The system comprises a physiological parameter acquisition unit, an environmental parameter acquisition unit, a data preprocessing unit, a feature extraction unit, a pneumonia diagnosis model unit, a decision early warning unit and a central processing unit. The method comprises the steps of collecting multi-modal data in real time, performing early diagnosis by a deep learning model after preprocessing and feature extraction, and generating early warning according to a result. According to the technical scheme, the physiological and environmental parameters of the down producing goats are comprehensively and continuously monitored in real time, the accuracy and reliability of early diagnosis are improved through deep learning, automatic early warning is achieved, the diagnosis period is shortened, and the morbidity and economic loss are reduced.

The application belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of a molecular marker related to a long hair trait of a cashmere goat. The molecular marker is located at 155,344,701-155,346,600 on a goat chromosome 1, and a nucleotide sequence thereof is shown as SEQ ID NO. 1. It is found by the application that the length of wool of an individual with a normal type of the molecular marker region is greater than the length of wool of an individual with a deletion type of the molecular marker region, and thus a cashmere goat breed with long wool can be selected by detecting a deletion mutation of the molecular marker on the goat chromosome 1.

The invention relates to the technical field of livestock breeding intellectualization, and discloses an intelligent breeding system and method for full-barn feeding cashmere goats. The method comprises the following steps: collecting position coordinates and attitude information of down producing goats in a breeding house, and collecting environmental parameters of a plurality of position points; identifying a sheep flock subset with a local aggregation situation according to the position coordinates, and synchronously obtaining environmental parameters associated with the geographic position of the subset; deducing an expected behavior evolution trend of the sheep flock subset under the action of the environmental parameters in combination with the attitude information and the associated environmental parameters; judging whether the distribution of the whole cashmere goats needs to be subjected to global intervention or not according to the trend; when it is judged that intervention is needed, the movement guiding priority of each cashmere goat is calculated; and generating a differentiated path guide strategy for each goat based on the priority and executing the strategy to adjust the distribution state of the down producing goats in the shed. According to the invention, active prediction and personalized accurate guidance of sheep flock behaviors are realized, and the intelligent level of breeding management and animal welfare are improved.

The utility model discloses a feed mixing device for down producing goats. The down producing goat feed mixing device comprises a stirring bin, supports are arranged on the two sides of the stirring bin, a motor is installed on the supports, a first screw rod and a second screw rod are symmetrically arranged on the inner wall of the stirring bin, and a third screw rod is arranged at the lower end of the first screw rod and the lower end of the second screw rod; the first screw rod, the second screw rod and the third screw rod are rotationally connected with the inner wall of the stirring bin respectively and can be driven by a motor, and the directions of left threads and right threads of the first screw rod, the second screw rod and the third screw rod are opposite respectively. According to the down producing goat feed mixing device, the first screw rod, the second screw rod and the third screw rod are arranged to achieve the function of smashing and stirring forage at the same time, herdsmen can use common forage such as straw to feed the forage needed by cattle and sheep in one step, and the time and cost for manufacturing the forage are saved.

The application provides sgRNA which specifically targets the Rosa26 safe site of a cashmere goat and application of using CRISPR / Cas9 to complete site-directed knockout and site-directed integration of an EGFP gene. The application firstly uses a bioinformatics method to predict the complete sequence of the Rosa26 site, then designs two sgRNA aiming at the site, constructs a targeting vector based on the CRISPR / Cas9 system, verifies the guiding efficiency of the sgRNA, simultaneously constructs a homologous integration EGFP gene vector, co-transfects the sgRNA and the homologous integration vector into cashmere goat fetal fibroblasts, and obtains a cell strain of the Rosa26 site site-directed integration of the EGFP gene. The efficiency of the sgRNA of the application in specifically guiding Cas9 to cut the Rosa26 site reaches about 40%, effectively reduces the off-target phenomenon existing in the CRISPR / Cas9 system, and further reduces the mutation of non-target gene sequences caused by non-specific cutting. The Cas9 / gRNA expression vector can realize the specific knockout or knock-in of the Rosa26 site at the cell, embryo or even individual level, so as to study the expression of specific genes and provide technical support for the cultivation of new goat breeds.

The present invention provides an sgRNA specifically targeting a cashmere goat safety site H11 and an application of site-directed knockout and site-directed integration of an EGFP gene mediated by a CRISPR / Cas9 system. The present invention first predicts the complete sequence of the H11 site (SEQIDNO.1) using a bioinformatics method, and then designs two sgRNAs (SEQIDNO.2 and 3) for the H11 site, and the DNA sequence of the sgRNA sequence transcribed is shown in SEQIDNO.4 or 5. A targeting vector based on the CRISPR / Cas9 system is constructed, and then the guiding efficiency of the sgRNA is verified by TA cloning sequencing, and a homologous integration EGFP gene vector is constructed at the same time, and finally the screened sgRNA (Cas9 / gRNA co-expression vector) and the homologous integration vector (H11-EGFP) are simultaneously transfected into cashmere goat fetal fibroblasts to obtain a cell line of the H11 site site-directed integration of the EGFP gene. The sgRNA of the present invention can specifically recognize the H11 site of cashmere goats, guide the Cas9 endonuclease to efficiently cut the site, and the targeting efficiency is above 50%, which can effectively reduce the off-target phenomenon in the CRISPR / Cas9 system, thereby reducing the mutation of non-target gene sequences caused by non-specific cutting. By constructing a Cas9 / gRNA expression vector, the specific modification (knockout or knock-in) of the H11 site can be achieved at the cell, embryo, or even individual level, thereby studying the expression of specific genes and providing technical support for the breeding of new breeds of goats.

The invention relates to the technical field of gene breeding, in particular to a detection method for insertion or deletion polymorphism of a GNG4 gene of a down producing goat and application of the detection method. The invention relates to an application of an insertion or deletion polymorphic site of a GNG4 gene NC030835.1: g.3706536937065370 of a down producing goat in goat growth trait-assisted selective breeding, which comprises the following steps: by taking a down producing goat whole genome to be detected as a template, amplifying the GNG4 gene through PCR (Polymerase Chain Reaction); carrying out agarose gel electrophoresis on the PCR amplification product, and identifying the mutation site according to an electrophoresis result; and carrying out assisted selective breeding on the growth traits of the goats according to the genotype of the mutation site. According to the application method for early breeding of the GNG4 gene InDel locus deletion / deletion polymorphic locus of the down producing goat, which is established by the invention, as an excellent growth character, the breeding process of an improved variety of the down producing goat can be accelerated.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The utility model provides a portable down producing goat oestrus detector. Comprising an inserting rod, a machine core shell is installed behind the inserting rod, a grip is installed behind the machine core shell, a liquid storage cavity is installed on the machine core shell, and a liquid conveying pipe is connected to the liquid storage cavity. By arranging the liquid storage cavity, the liquid conveying pipe, the liquid conveying plate, the connecting pipe, the mounting plate, the micro water pump, the liquid flow channel and the liquid outlet hole, the automatic lubricating function of the front end of the inserting rod can be achieved, the liquid outlet hole enables lubricating liquid to evenly cover the front end of the inserting rod, then a lubricating film with a certain thickness is formed at the front end of the inserting rod, friction force is effectively reduced, and the service life of the inserting rod is prolonged. The device is simple in structure and convenient to use, pain caused by friction is weakened, non-uniformity of traditional manual smearing is avoided, mucous membrane injury risks caused by friction are remarkably reduced, the situation that the down producing goats are subjected to stress collision is reduced, and the device is small in size, high in integration degree and excellent in portability at the same time.

The invention is suitable for the technical field of biology, and provides a molecular marker related to the diameter character of cashmere of Alba cashmere goats and application of the molecular marker, the molecular marker comprises at least one or a combination of 17 SNP sites and 5 InDel sites which are screened based on whole genome association analysis and located on a No.16 chromosome, and by detecting the molecular marker, the diameter character of the cashmere of the Alba cashmere goats can be detected, and the diameter character of the cashmere of the Alba cashmere goats can be detected. The method can quickly and effectively predict the diameter character of cashmere of the cashmere goat at low cost, and has important significance in reducing the diameter of cashmere and improving the quality of cashmere. By applying the molecular marker, breeding is assisted through the molecular marker, favorable genotype individuals are reserved, and unfavorable genotype individuals are eliminated, so that the cashmere diameter of a group is reduced, the fineness of fluff is improved, the purpose of improving the quality of the fluff is achieved, and the market demand is met.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The invention discloses a simple device for increasing the cashmere yield of cashmere goats.The simple device for increasing the cashmere yield of the cashmere goats.The simple device for increasing the cashmere yield of the cashmere goats.The simple device for increasing the cashmere yield of the cashmere goats.The simple device comprises a breeding shed, a feeding frame is fixedly connected to the surface of the breeding shed, a sliding groove is formed in the surface of the feeding frame, and a sliding plate is slidably connected to the inner wall of the sliding groove; when the motor is started, an output shaft of the motor rotates to drive a first belt wheel to rotate, then a belt is driven to drive a second belt wheel to rotate, then a rotating shaft is driven to rotate on the inner wall of the sliding groove, the belt moves to drive a fixing block to move, and then the sliding plate is driven to slide on the inner wall of the sliding groove. And when feed is put into the breeding shed from the outside of the breeding shed and is transmitted into the breeding shed, the safety of a breeder is guaranteed, and the breeder can feed a flock of sheep more easily.

This invention relates to the field of intelligent livestock farming technology, and discloses a fully automated intelligent feeding method for cashmere goat farming. The method includes: monitoring environmental parameters of the cashmere goat activity area and obtaining corresponding physiological parameters to construct an environmental-physiological dynamic correlation model; determining, based on the model, a data acquisition command to trigger feeding decisions when environmental parameters change; periodically acquiring multi-dimensional observation data containing spatiotemporal information of different subgroups within the area according to the command; extracting behavioral segments representing individual behavior from the data, analyzing their attributes, and classifying them into different feeding decision stages; allocating dynamic feed ratios to subgroups corresponding to each behavioral segment based on the feeding decision stage and a preset feed ratio library; and integrating the ratios of each subgroup to generate a global feeding plan. This invention achieves fully automated intelligent feeding from environment-driven to precise behavior recognition.

The utility model provides an anti-freezing device for breeding down producing goats in a high altitude area. Comprising a water tank; the device is characterized in that one end of the water tank is fixedly connected with a water supplementing tank, the other end of the water tank is fixedly connected with a control box, the top of the water supplementing tank is provided with a locking set, the lower end of the locking set is fixedly connected with a connecting line, the lower end of the connecting line is fixedly connected with a floating ball, and the lower end of the floating ball is fixedly connected with a connecting rod. A conical plug is fixedly connected to the lower end of the connecting rod, an external water pipe is arranged at the bottom end of the water supplementing tank, heating boxes are arranged on the two sides of the water tank, and a temperature controller is arranged at the bottom in the water tank. The goat water heater has the advantages that when the water temperature reaches the set temperature suitable for drinking of goats, the temperature controller cuts off the circuit to stop heating of the heating pipe, the working principle similar to that of a water dispenser can not only achieve the purpose of preventing water from freezing, but also prevent the water temperature from being too high, and meanwhile more electric energy is saved.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.