26 results about "Cashmere goat" patented technology

The invention relates to the technical field of livestock breeding intellectualization, and discloses an intelligent breeding system and method for full-barn feeding cashmere goats. The method comprises the following steps: collecting position coordinates and attitude information of down producing goats in a breeding house, and collecting environmental parameters of a plurality of position points; identifying a sheep flock subset with a local aggregation situation according to the position coordinates, and synchronously obtaining environmental parameters associated with the geographic position of the subset; deducing an expected behavior evolution trend of the sheep flock subset under the action of the environmental parameters in combination with the attitude information and the associated environmental parameters; judging whether the distribution of the whole cashmere goats needs to be subjected to global intervention or not according to the trend; when it is judged that intervention is needed, the movement guiding priority of each cashmere goat is calculated; and generating a differentiated path guide strategy for each goat based on the priority and executing the strategy to adjust the distribution state of the down producing goats in the shed. According to the invention, active prediction and personalized accurate guidance of sheep flock behaviors are realized, and the intelligent level of breeding management and animal welfare are improved.

The utility model discloses a feed mixing device for down producing goats. The down producing goat feed mixing device comprises a stirring bin, supports are arranged on the two sides of the stirring bin, a motor is installed on the supports, a first screw rod and a second screw rod are symmetrically arranged on the inner wall of the stirring bin, and a third screw rod is arranged at the lower end of the first screw rod and the lower end of the second screw rod; the first screw rod, the second screw rod and the third screw rod are rotationally connected with the inner wall of the stirring bin respectively and can be driven by a motor, and the directions of left threads and right threads of the first screw rod, the second screw rod and the third screw rod are opposite respectively. According to the down producing goat feed mixing device, the first screw rod, the second screw rod and the third screw rod are arranged to achieve the function of smashing and stirring forage at the same time, herdsmen can use common forage such as straw to feed the forage needed by cattle and sheep in one step, and the time and cost for manufacturing the forage are saved.

The application provides sgRNA which specifically targets the Rosa26 safe site of a cashmere goat and application of using CRISPR / Cas9 to complete site-directed knockout and site-directed integration of an EGFP gene. The application firstly uses a bioinformatics method to predict the complete sequence of the Rosa26 site, then designs two sgRNA aiming at the site, constructs a targeting vector based on the CRISPR / Cas9 system, verifies the guiding efficiency of the sgRNA, simultaneously constructs a homologous integration EGFP gene vector, co-transfects the sgRNA and the homologous integration vector into cashmere goat fetal fibroblasts, and obtains a cell strain of the Rosa26 site site-directed integration of the EGFP gene. The efficiency of the sgRNA of the application in specifically guiding Cas9 to cut the Rosa26 site reaches about 40%, effectively reduces the off-target phenomenon existing in the CRISPR / Cas9 system, and further reduces the mutation of non-target gene sequences caused by non-specific cutting. The Cas9 / gRNA expression vector can realize the specific knockout or knock-in of the Rosa26 site at the cell, embryo or even individual level, so as to study the expression of specific genes and provide technical support for the cultivation of new goat breeds.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The utility model provides a portable down producing goat oestrus detector. Comprising an inserting rod, a machine core shell is installed behind the inserting rod, a grip is installed behind the machine core shell, a liquid storage cavity is installed on the machine core shell, and a liquid conveying pipe is connected to the liquid storage cavity. By arranging the liquid storage cavity, the liquid conveying pipe, the liquid conveying plate, the connecting pipe, the mounting plate, the micro water pump, the liquid flow channel and the liquid outlet hole, the automatic lubricating function of the front end of the inserting rod can be achieved, the liquid outlet hole enables lubricating liquid to evenly cover the front end of the inserting rod, then a lubricating film with a certain thickness is formed at the front end of the inserting rod, friction force is effectively reduced, and the service life of the inserting rod is prolonged. The device is simple in structure and convenient to use, pain caused by friction is weakened, non-uniformity of traditional manual smearing is avoided, mucous membrane injury risks caused by friction are remarkably reduced, the situation that the down producing goats are subjected to stress collision is reduced, and the device is small in size, high in integration degree and excellent in portability at the same time.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

This invention relates to the field of intelligent livestock farming technology, and discloses a fully automated intelligent feeding method for cashmere goat farming. The method includes: monitoring environmental parameters of the cashmere goat activity area and obtaining corresponding physiological parameters to construct an environmental-physiological dynamic correlation model; determining, based on the model, a data acquisition command to trigger feeding decisions when environmental parameters change; periodically acquiring multi-dimensional observation data containing spatiotemporal information of different subgroups within the area according to the command; extracting behavioral segments representing individual behavior from the data, analyzing their attributes, and classifying them into different feeding decision stages; allocating dynamic feed ratios to subgroups corresponding to each behavioral segment based on the feeding decision stage and a preset feed ratio library; and integrating the ratios of each subgroup to generate a global feeding plan. This invention achieves fully automated intelligent feeding from environment-driven to precise behavior recognition.

The utility model provides an anti-freezing device for breeding down producing goats in a high altitude area. Comprising a water tank; the device is characterized in that one end of the water tank is fixedly connected with a water supplementing tank, the other end of the water tank is fixedly connected with a control box, the top of the water supplementing tank is provided with a locking set, the lower end of the locking set is fixedly connected with a connecting line, the lower end of the connecting line is fixedly connected with a floating ball, and the lower end of the floating ball is fixedly connected with a connecting rod. A conical plug is fixedly connected to the lower end of the connecting rod, an external water pipe is arranged at the bottom end of the water supplementing tank, heating boxes are arranged on the two sides of the water tank, and a temperature controller is arranged at the bottom in the water tank. The goat water heater has the advantages that when the water temperature reaches the set temperature suitable for drinking of goats, the temperature controller cuts off the circuit to stop heating of the heating pipe, the working principle similar to that of a water dispenser can not only achieve the purpose of preventing water from freezing, but also prevent the water temperature from being too high, and meanwhile more electric energy is saved.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.

The invention belongs to the technical field of animal nutrition and feed, and particularly relates to a feed composition, a method for improving fattening performance and meat quality of down producing goats and application, corn straw is used for replacing 40-60% of high-quality forage grass in basic daily ration of the down producing goats, and artemisia ordosica crude polysaccharide accounting for 0.08-0.12% of the total dry matter weight of the daily ration is added; experimental results show that the feed composition can significantly improve the average daily gain (up to 45.25%) of the down producing goats, reduce the feed conversion ratio (up to 27.32%), and improve the apparent digestibility of nutrient substances, the rumen fermentation function and nitrogen metabolism; meanwhile, the slaughter performance can be improved, the meat quality can be improved, physiological and biochemical indexes of blood can be adjusted, and immunity and metabolic health can be enhanced; efficient utilization of corn straw resources is achieved, the problem of shortage of high-quality roughage is solved, and remarkable economic and ecological benefits are achieved.

This invention belongs to the field of animal genetic engineering technology, specifically relating to a cashmere goat circRNA, an interference vector, and their application in regulating cashmere goat hair follicle development. The cashmere goat circRNA is circ-0000987; the base sequence of circ-0000987 is shown in SEQ ID NO.1, and it is used to regulate cashmere goat hair follicle development. This invention reveals for the first time the inhibitory effect of circ-0000987 on the proliferation of cashmere goat papillary cells. The results of this invention show that circ-0000987 plays a proliferative inhibitory role in cashmere goat papillary cells, inhibiting their activity by negatively regulating cell proliferation-related pathways; and participating in the transition of hair follicles from the anagen to catagen phase by inhibiting cell proliferation and promoting apoptosis.

The present application belongs to the technical field of livestock breeding, and particularly relates to a breeding ecological shed for cashmere goats, comprising a shed, a footboard, a manure leakage tank and a dredging device, the dredging device comprising a main plate, a driving structure and a plurality of dredging assemblies, the dredging assembly comprising a guide head, in the present application, when the guide head is located directly above the manure leakage tank, the guide head will be in contact with the goat manure blocked in the manure leakage tank in the process of moving downward, at the same time, the goat manure blocked in the manure leakage tank will exert a reverse force on the guide head, as the anti-deviation sleeve continues to slide downward, the impact spring will be gradually compressed, at this time, the elastic potential energy of the impact spring gradually increases, when the elastic potential energy accumulated by the impact spring reaches a certain degree, the elastic force generated by the impact spring is sufficient to overcome the resistance formed by the goat manure blockage, at this time, the impact spring starts to stretch and pushes the cross limiting sliding block downward, so that the guide head extends through the goat manure to the lower side of the manure leakage tank.

According to the optimal algorithm framework method for genome selective breeding of the villus sheep, standardized phenotype data are collected in a unified environment, genotyping with the SNP density not lower than 500,000 is adopted, strict quality control standards and Beagle high-precision interpolation are combined, and the genotype data integrity and accuracy are remarkably improved. According to the method, a structured database containing individual IDs, pedigree, phenotypes, genotypes and quality control logs is constructed, version management and programmed calling are supported, and systematic management and long-term accumulation of breeding data are achieved. According to the framework, the genome prediction accuracy is improved, early seed selection and recessive excellent character improvement are realized, the generation interval is shortened, the genetic progress is accelerated, and the framework has good generalizability and industrial application prospects and is suitable for efficient genetic improvement of main villus sheep varieties such as villus goats and fine-wool sheep.

The invention provides a daily ration formula for improving fattening performance and meat quality of down producing goats and application of the daily ration formula, and belongs to the technical field of animal nutrition and feed. Corn straw and caragana microphylla are used for jointly replacing 40-60% of high-quality forage grass, 0.07-0.13% of artemisia ordosica crude polysaccharide is added, the average daily gain of the down producing goats is remarkably increased, the feed conversion ratio is reduced, meanwhile, the feed conversion rate is increased, and meanwhile the fattening performance and meat quality of the down producing goats are improved. The meat quality is improved, including reduction of muscle water loss rate and cooking loss, increase of meat color redness value and the like; besides, the formula also improves the digestibility of nutrient substances, especially the digestibility of crude protein, improves the rumen fermentation function, and improves the concentrations of rumen ammoniacal nitrogen and mycoprotein. Experiments show that the daily ration formula not only achieves the feeding effect similar to that of high-quality pasture, but also remarkably reduces the feeding cost, improves the economic benefit and provides a scientific basis for barn feeding breeding of down producing goats.

The invention discloses an SNP (Single Nucleotide Polymorphism) site set for accurately identifying germplasm resources of down producing goats and a special chip thereof, and belongs to the technical field of molecular genetic breeding. The SNP locus set comprises 10,000 differential SNP loci, is obtained by screening whole genome re-sequencing data of 43 individuals of Inner Mongolia down producing goats (Alba type and Arabic type), Liaoning down producing goats, southern Xinjiang down producing goats and northwest Tibetan white down producing goats, and is clear in chromosome distribution, suballele frequency and functional region. According to the special liquid chip prepared on the basis of the site set, the probe length is 120 bp, the design conforms to specific parameters, the average detection rate of samples and sites exceeds 99% when the sequencing depth is larger than 5 *, five cashmere goat varieties can be accurately distinguished through principal component analysis and phylogenetic tree construction, and technical support is provided for germplasm resource protection, breeding improvement and germplasm innovation of the cashmere goats.

This invention relates to the field of biotechnology, and more particularly to the application of the chi-miR-1388-3p gene in the melatonin-mediated regulation of cashmere growth in cashmere goats. This invention discovers a microRNA (chi-miR-1388-3p) associated with the growth cycle of cashmere goat hair follicles, which can regulate the proliferation, cell cycle, or apoptosis of hair papilla cells in cashmere goat skin, thereby regulating cashmere goat hair follicle growth and cashmere growth. Furthermore, this invention is the first to discover that melatonin-mediated interference with chi-miR-1388-3p promotes the proliferation of hair papilla cells in cashmere goat skin, regulates the cell cycle, and inhibits apoptosis, providing valuable insights into the regulatory role of melatonin-mediated miRNAs in cashmere goat hair follicle growth.

The application discloses a kind of fine feeding of increasing down of cashmere goat and preparation method thereof, by weight parts, consisting of the following components: 30-40 parts of puffed corn flour, 10-20 parts of puffed soybean, 8-10 parts of fermented wheat bran, 5-8 parts of linseed meal, 8-10 parts of alfalfa grass granules, 3-5 parts of Antarctic krill powder, 4-6 parts of wine yeast culture, 3-5 parts of inorganic salt, 0.01-0.03 parts of nano selenium, 1-3 parts of silkworm chrysalis powder, 3-5 parts of organic nanocrystal active ingredient, 6000-10000 IU / kg of compound vitamin, 0.3-0.6 parts of L-cysteine, 0.1-0.3 parts of gamma-aminobutyric acid, 50-70 mg / kg of nano zinc, 10-20 mg / kg of amino acid chelated copper, 25-40 mg / kg of yeast manganese, 1-2 parts of compound premix.The feed can significantly improve the yield of down and cashmere quality, stability, palatability and digestibility are good.

The invention relates to the technical field of animal nutrition and feed, and discloses a functional low-protein daily ration for down producing goats, which is composed of a basic daily ration with crude protein content of 9.0-10.0% and an additive formed by mixing rumen bypass L-glutamine particles and L-citrulline. Aiming at the problem of reproduction rate reduction caused by low-protein daily ration confirmed for the first time, the intestinal tract is repaired through the L-glutamine, and the L-glutamine and the L-glutamine can be metabolized and converted into arginine in vivo, so that the nitric oxide level and related reproductive hormone secretion are improved. The daily ration effectively solves the problems of low nitrogen utilization rate and reduced reproductive capacity of the down producing goats under the low-protein miscellaneous meal feeding condition, remarkably improves the conception rate and the nitrogen deposition level, and has the effects of reducing the cost and reducing emission.

The invention provides a nutrition and heredity coordinated regulation method for improving reproductive performance of downy sheep, which comprises the following steps: collecting ear tissue or blood samples, detecting BMPR1B, GDF9, PRLR and ESR1 key reproductive gene loci, screening individuals carrying dominant genotypes to establish a high-yield core group, and increasing the target gene frequency to 65% or more. Performing differentiated nutrition regulation and control by combining genotypes: performing short-term optimal feeding before hybridization, and improving energy, protein, beta-carotene, vitamin E and an organic selenium additive; a BLUP and machine learning are utilized to optimize a regulation and control strategy, and precise and data-driven closed-loop management of reproductive performance is realized. According to the cooperative regulation method, the estrus rate, the conception rate, the lambing number and the lamb survival rate of the down sheep can be remarkably increased, the breeding interval is shortened, and the method is suitable for efficient breeding system construction of special economic sheep species of down producing goats and down producing sheep and has wide application and popularization prospects.

The application relates to the feed technology field and discloses a low-protein amino acid balanced daily feed for cashmere goats and a preparation method thereof. The daily feed is prepared from 11-20 parts of crystal amino acid, 10-20 parts of feed-grade montmorillonite, 0.5-1.5 parts of anhydrous citric acid, 5-12 parts of condensed tannin, 1-3 parts of zinc sulfate heptahydrate, 10-25 parts of calcium lignosulfonate and 910-950 parts of basic low-protein daily feed powder according to weight proportions. During preparation, the crystal amino acid is mixed with the montmorillonite, and the self-assembly coating is completed by adding citric acid aqueous solution and condensed tannin in sequence, the zinc sulfate solution is sprayed to in-situ synthesize a hybrid core premix, finally, the basic powder and the calcium lignosulfonate are mixed, and the daily feed is prepared by steam conditioning and extrusion shaping. The daily feed is prepared by using intercalation, coordination crosslinking and heat sealing, the over-rumen protection rate and the processing retention rate of the amino acid can be improved, and the amino acid balance under the low-protein condition is realized.

This invention belongs to the field of goat vaccine technology and is an application of a vaccine for the prevention of small ruminant plague (PPR) and sheep pox. This PPR-sheep pox vaccine is an improvement on the injection method of a bivalent live PPR-sheep pox vaccine, suitable for doubling the intramuscular injection in goats. Each injection of 1 ml of the bivalent live PPR-sheep pox vaccine results in a population antibody titer of 70% for both PPR and sheep pox after 30 days, and sheep pox antibody titer can still be detected after 180 days. This PPR-sheep pox vaccine demonstrates good safety and PPR immunity protection in Alashan white cashmere goats. Furthermore, the vaccine is suitable for intramuscular injection, which meets the needs of sheep pox immunization and control, and is suitable for practical grassroots disease prevention operations. It provides important technical support for the scientific prevention and control of diseases in regional white cashmere goats. Promoting the use of this PPR-sheep pox vaccine can prevent both PPR and sheep pox.

The application belongs to the technical field of molecular genetics, and particularly relates to a detection method of an insertion / deletion marker of a Shanbei white cashmere goat ASIC2 gene and application thereof. The application uses the designed amplification primer pair, takes the whole genome DNA of the cashmere goat to be detected as a template, amplifies the 5' regulatory region fragment (NC_030826.1:g.16746415_16746441) of the cashmere goat ASIC2 gene through PCR, and then performs agarose gel electrophoresis and sequencing technology to identify the genotype of the insertion / deletion polymorphism site of the cashmere goat ASIC2 gene NC_030826.1:g.16746415_16746441del. The results of the examples show that the different genotypes of the InDel site of the cashmere goat ASIC2 gene are significantly related to the chest depth and cross section height of the Shanbei white cashmere goat, and can be used as an effective DNA marker of the growth traits of the Shanbei white cashmere goat. Further, the DNA marker can assist in breeding excellent cashmere goats.

The present application belongs to the technical field of livestock breeding, and particularly relates to a detection method of an insertion / deletion marker of an IRF3 gene of a Shaanbei white cashmere goat and application thereof. The present application takes whole genome DNA of the Shaanbei white cashmere goat as a template, designs a primer combination based on a specific sequence of a regulation region of the IRF3 gene, and realizes efficient, accurate and economical detection of an insertion / deletion polymorphism of a target gene by using molecular biology technologies such as polymerase chain reaction amplification, agarose electrophoresis separation and direct sequencing analysis. The present application is the first to find that the IRF3 gene is significantly related to brucellosis resistance of the Shaanbei white cashmere goat, and can be used as an effective DNA marker for genetic breeding of the Shaanbei white cashmere goat against brucellosis.

The utility model is suitable for the technical field of livestock breeding, and provides a cashmere goat breeding and cleaning device which comprises a movable vehicle body, and a water tank is fixed to the top of the vehicle body and used for containing water for cleaning a cashmere goat breeding pen. The high-pressure water pump is arranged on the vehicle body, the water inlet end of the high-pressure water pump is fixedly communicated with the water tank through a water pumping pipe, and the high-pressure water pump is used for pumping cleaning water. According to the cleaning device for breeding of the down producing goats, high-pressure water flow can be generated through combination of the high-pressure water pump and the cleaning gun head, excrement, dust and other dirt in a breeding pen are deeply cleaned, and the cleaning quality is ensured; and the filter box and the filter box are introduced to filter water added into the water tank, so that impurities in the water are effectively removed, and a water supply pipeline and the cleaning gun head are prevented from being blocked.

The application belongs to the technical field of genetic breeding, and particularly relates to a SNP marker affecting fine-bundle large cashmere goats and application thereof. The SNP marker comprises marker one and / or marker two. The marker one and / or the marker two affect the fine-bundle large of cashmere goats. The marker one is located at 48854986 of chromosome 6 of a cashmere goat genome, and a G to A mutation occurs. The marker two is located at 76025546 of chromosome 14 of the cashmere goat genome, and a T to C mutation occurs.

The invention belongs to the technical field of livestock breeding, and particularly relates to a down producing goat breeding ecological shed which comprises a shed body, a pedal, a manure leaking groove and a dredger, the dredger comprises a main plate, a driving structure and a plurality of hole dredging assemblies, and each hole dredging assembly comprises a guide head. When the anti-deviation sleeve slides downwards, the anti-deviation sleeve can make contact with sheep manure blocked in the manure leakage groove, meanwhile, the sheep manure blocked in the manure leakage groove can exert reverse acting force on the guide head, the anti-deviation sleeve continues to slide downwards, the impact spring can be gradually compressed, and at the moment, the elastic potential energy of the impact spring is gradually increased; when the elastic potential energy accumulated by the impact spring reaches a certain degree and the generated elastic force is enough to overcome the resistance formed by sheep manure blockage, the impact spring starts to stretch and downwards pushes the cross-shaped limiting sliding block, so that the guide head penetrates through the sheep manure and extends to the lower part of the sheep manure leakage groove.