93 results about "Cashmere goat" patented technology

The invention belongs to the field of establishment of a population genetic resource bank, and provides a scientific establishment method of a goat resource population bank, and provides a goat reference family system establishment case, and relates to two national-level genetic resource varieties, namely, a Dazu black goat and an Inner Mongolia Cashmere goat (Alxa type). According to the resource population establishment method, forward cross and reciprocal cross of Dazu black goat and Inner Mongolia Cashmere goat (Alxa type) are utilized, random mating of genetic relationship is avoided for F1, so that Mendel genetic characters are separated, a goat economic character QTL or a functional gene is located by virtue of a genome means, and a relatively-precise genetic mark is provided for the goat breeding. The genetic resource bank comprises goat population, a genealogy inforamtion database, a phenotypic character database, a DNA sample library, an RNA sample library and a genetic variation information database.

The present invention relates to the technical field of animal breeding, in particular to a breeding method of the meat-cashmere goat, which resolves the problems of the prior meat goat and the prior Cashmere goat that the meat output and the cashmere output are not balanced. The present invention firstly selects the breeding goat of the Inner Mongolian cashmere goat to be mated with the local excellent-quality original goat to get the first generation of the hybrid variety; and then selects the KELAN breeding cashmere goat, the Asian gazelle, the Boer goat, the cashmere goat in Gaizhou of Liaoning and the new generation of Liaoning cashmere as the male parent to mate with the offspring of the excellent hybrid goat so as to finally get a new variety of the meat-cashmere goat. The present invention adopts the diversity hybrid way to form a new goat strain both for the meat and the cashmere after breeding in more than twenty years, which integrates the advantages of the meat goat and the cashmere goat and has the advantages that the goat is heavy when born, the weight is heavy and the growth is fast; the meat yield and the cashmere yield are high; the sex maturation is early and the production rate is high; after breeding in large scale, the present invention has important significance for improving the life of the people and developing the cultivation industry, which has a favorable economic benefit and the social benefit.

The invention discloses a detection method for an insertion/deletion marker of a goat gene IGF2BP1. The detection method comprises the following steps: with to-be-detected goat whole-genome DNA as a template, applying a part of fragments of the goat gene IGF2BP1 by PCR, then carrying out agarose gel electrophoresis, and identifying the genotypes of the 15-bp insertion/deletion polymorphic site andthe 5-bp insertion/deletion polymorphic site of the goat gene IGF2BP1 according to electrophoresis results. According to the invention, different genotypes and a combined genotype of 15-bp and 5-bp insertion/deletion polymorphic sites of the goat gene IGF2BP1 have significant correlational relationship with the litter size character of Shaanbei white-cashmere goat, so the 15-bp and 5-bp insertion/deletion polymorphic sites can be used as DNA markers to improve the litter size character of the goat. The detection method for the insertion/deletion marker of the goat gene IGF2BP1 provided by theinvention can be applied to goat molecular marker-assisted selective breeding, and accelerates establishment of an excellent goat genetic resource population.

The invention relates to an InDel molecular marker related to cashmere traits of goat and an application of the InDel molecular marker. The marker is located between the 95454685bp and the 95455189bpof the No.6 chromosome of the goat; the polymorphism of the InDel molecular marker is -/insertion fragment; and a sequence of the insertion fragment is shown as SEQ ID NO.1. The invention also provides specific primers and a method for detecting the InDel marker; and sequences of the specific primer pair are shown as SEQ ID NO.2-3. Through the specific primer pair provided by the invention, on thebasis of an electrophoretic band obtained through PCR and agarose gel electrophoresis as well as analysis and statistics on Sanger sequencing and in accordance with cashmere goat allele and non-cashmere goat allele types on an InDel site, it can identify cashmere or non-cashmere traits of a goat variety. The InDel molecular marker provided by the invention is low in amount of detected samples inneed, simple and convenient in method and accurate in identifying result, and the InDel molecular marker has a good prospect of application and promotion.

The invention discloses a detection method for goat CSN1S1 gene insertion/deletion, and applications thereof. According to the method, the whole genomic DNA of a goat to be detected is used as a template, the goat CSN1S1 gene is amplified through a PCR technology, and agarose gel electrophoresis is performed to identify whether the indel polymorphism exists in the goat CSN1S1 gene. According to the present invention, the large sample (N is more than 1000) related analysis results reveal that the different types of the CSN1S1 gene indel are significantly related to the first lambing trait of Shanbei white cashmere goat, such that the different types of the CSN1S1 gene indel can be used as the DNA mark for improving the first lambing trait of the goat; and the method for rapidly detecting the goat CSN1S1 gene indel can be used in the marker-assisted selection breeding of goat reproductive traits in China, and is conducive to the rapid establishment of the goat genetic resource groups having the high lambing number trait.

The invention discloses a detection method of goat CMTM2 gene insertion/deletion polymorphism and an application thereof. According to the invention, by using the whole genome DNA of the goat to-be-tested as a template, a partial fragment of the goat CMTM2 gene is amplified by PCR, and then agarose gel electrophoresis is carried out, According to the electrophoresis results, the genotype at a 14-bp insertion/deletion polymorphism site of the goat CMTM2 gene NC_030825.1: g.35582961_35582974 site is identified. Correlation analysis shows that the different genotypes of 14-bp insertion/deletion polymorphism of the goat CMTM2 gene and the property of litter size of Shanbei white cashmere goats have a significant correlation, and DNA markers capable of increasing the property of litter size areexisted. The method can be applied to goat molecular marker-assisted selection breeding, and accelerates the establishment of an excellent goat genetic resource group.

The invention discloses an application of a KAP26.1 gene as an exogenous gene introduced into cashmere goat cells and used for improving wool fineness due to the fact that the KAP26.1 gene produces a regulating effect on Liaoning cashmere goat wool fineness, that is, the higher the expression quantity of the KAP26.1 gene is, the smaller the wool fineness is, meanwhile, the high expression of the KAP26.1 gene can prompt increase of expression quantity of a KAP7.1 gene, KAP11.1 gene and the like, the wool fineness can be reduced with the gene increase, and accordingly, the wool quality is improved. According to the application, KAP26.1 gene overexpression lentivirus is injected into the testis of a Liaoning cashmere goat, after the KAP26.1 gene is taken as the exogenous gene to be introduced into the Cashmere goat cells through virus infection, a Liaoning cashmere goat individual with high KAP26.1 gene expression quantity is selected and bred as a breeding goat, so that the cashmere goat wool fineness can be improved, and the wool quality is increased.

The invention discloses a genetic marker related to a cashmere wool property of eastern Gansu cashmere goats. The genetic marker is a partial DNA (Deoxyribonucleic Acid) sequence of a goat KRTAP20-2 gene; a nucleotide sequence of the genetic marker is shown as SEQ ID No: 1 to SEQ ID No: 3 in a sequence table; the invention provides an amplification primer and application of the genetic marker. Thegenetic marker disclosed by the invention has the beneficial effects that a KRTAP20-2 gene type and an allele have extremely remarkable or remarkable influences on the wool yield and the wool layer height of the eastern Gansu cashmere goats. The wool yield of individuals containing an allele A is 38g higher than that of individuals without the allele A; the wool yield and the wool layer height ofindividuals containing an allele B are 30g and 0.1cm lower than those of individuals without the allele B respectively. Therefore, when the wool yield of the eastern Gansu cashmere goats is subjectedto breed selection, the individuals containing the allele A need to be remained, and the individuals containing the allele B are weeded out, so that the aim of improving the wool yield of the easternGansu cashmere goats is realized.

The invention discloses a goat HIAT1 gene insertion/deletion polymorphism detection method and application thereof. A whole genome DNA of a to-be-detected goat serves as a template, and a goat HIAT1 gene part fragment is amplified through a PCR; agarose gel electrophoresis is carried out, and the genotypes of 15-bp insertion/deletion polymorphism loci at goat HIAT1 gene NC_030810.1:g.7695-7696 bits are identified through electrophoresis results. Related analysis results show that different genotypes of goat HIAT1 gene 15-bp insertion/deletion polymorphism have a remarkable correlation with growth characters of Shannxi white cashmere goats and can serve as DNA markers for improving the growth characters of the goats. The goat HIAT1 gene insertion/deletion polymorphism detection method can be applied to goat molecular marker assisted selective breeding so as to accelerate the establishment of a goat genetic resource group with excellent growth characters.

The invention relates to auxiliary researches of protein localization in the technical field of animal histology, particularly a preparation and dyeing method of a paraffin section for microscopic observation. The preparation method is mainly characterized by comprising the following steps: taking a skin sample; fixing the sample; softening the skin sample; dehydrating the skin sample; carrying out paraffin treatment; carrying out conventional embedment on the skin sample; and sectioning the skin sample. The method is simple in process and convenient to operate; and the obtained section is complete and has clear picture structure. The method is suitable for laboratory researchers to research the morphological structure of cashmere goat skin. When being observed under a light microscope, the paraffin section can completely display the epidermis and derma structures of the skin, does not have wrinkle or crushing, and can not easily have the phenomenon of crushing in the sectioning process.

The invention is suitable for the field of a biological technology, and provides a molecular marker related with cashmere fineness trait and a detection primer and application of the molecular markerrelated with cashmere fineness trait. The molecular marker comprises a G1355A site of a down-producing goat NFKBIA gene, wherein the nucleotide sequence of the G1355A site is shown as a sequence tableSEQID NO:1, and R in the nucleotide sequence of the G1355A site is A or G. The advantageous genes of the G1355A site for cashmere fineness, cashmere yield, cashmere length and net cashmere rate are AG genetype. Therefore, the AG genetype of the G1355A site can be used for breeding of fine-cashmere down-producing goats to improve the condition that the quality of the cashmere of the down-producinggoats of Liaoning province is poor.

The invention discloses a detection method and application of goat ATBF1 (AT motif binding factor 1) gene indel (insertion/deletion) polymorphism and an application of the detection method. The wholegenome DNA of a to-be-detected goat is taken as a template, primer pairs P1 and P2 are taken as primers, part of fragments of the goat ATBF1 gene is amplified with a PCR (polymerase chain reaction) technology, and then AGE (agarose gel electrophoresis) is performed for identifying the indel site polymorphism of the goat ATBF1 gene. Correlation analysis results reveal that different genotypes of the indel site of the ATBF1 gene are significantly associated with the body height and chest circumference of northern Shaanxi white cashmere goats, Guanzhong milk goats and Hainan black goats and disclose that the genotype of the indel site of ATBF1 gene can be used as DNA markers for improving the body height and chest circumference of the goats. The detection method can be applied to goat marker-assisted selection breeding and is good for quickly establishing goat genetic resource populations with excellent body size characteristics.

The invention discloses a detection method of insertion/deletion polymorphism of a goat DNAH1 gene and an application of the detection method. The DNA of goat complete genome to be detected is used asa formwork, through PCR, a part of fragment of the goat DNAH1 gene is amplified, then agarose gel electrophoresis is performed, and according to the electrophoresis result, the genotype of insertion/deletion polymorphism site of NC_030829.1:g.48594103_48594129 site 27-bp of the goat DNAH1 gene is identified. According to the correlation analysis result, the inventor finds that different genotypesof the 27-bp insertion/deletion polymorphism of the goat DNAH1 gene has significant correlation relationship with lambing number traits of Shanbei white cashmere goats, and a DNA marker capable of increasing goat lambing number trait exists. The method for detecting the insertion/deletion polymorphism of the goat DNAH1 gene provided by the invention can be applied to molecular marker assisted selective breeding of goats, and establishing of good goat heredity resource groups is accelerated.

The invention provides a complete feed for adult cashmere goat. The feed comprises the following raw materials in parts by weight: 35-40 copies of corn stalks, 18-30 parts of soybean skin, 5-10 parts of bran, 10-20 parts of corn bran, 20-30 parts of alfalfa, 0.4-0.6 part of edible salt, 35 parts of corn stalks, 20 parts of soybean skin, 8 parts of bran, 12 parts of corn bran, 24.6 parts of alfalfa and 0.4 parts of edible salt. The feed provided by the invention can be used for fully meeting the nutritional needs of cashmere goat diets under full barn feeding conditions, is high in fattening efficiency and rapid in growth of goat, and does not use any refined grains and additives, and the barn feeding cost is greatly reduced.

The invention discloses a method for detecting of goat DNMT3B gene insertion/deletion polymorphism and application of the method. A goat whole genome DNA to be detected is used as a template, and partial fragments of the goat DNMT3B gene are amplified by PCR; then agarose gel electrophoresis is performed, according to an electrophoreticthe result, genotypes of a goat DNMT3B gene NC_030820.1:g.61500477_61500478 locus 11-bp insertion/deletion polymorphism locus are identified. A significant correlation exists between the different genotypes of the 11-bp insertion/deletion polymorphism locus andthe litter size traits of Northern Shaanxi white cashmere goat, and a DNA marker exists to improve the litter size traits. The method for detecting the goat DNMT3B gene insertion/deletion polymorphismcan be applied to goat molecular marker assisted selection breeding to accelerate the establishment of excellent goat genetic resource population.

The present invention discloses a cashmere goat polyembryonic new strain breeding method which includes the following steps: selecting and collecting polyembryonic cashmere goats as basic groups, and carrying out enclosed breeding of the cashmere goats, selecting excellent offsprings to build basic groups again, carrying out enclosed breeding of the selected the excellent offsprings, selecting excellent individuals from the offsprings through the enclosed breeding as core groups, carrying out enclosed breeding of the core groups, selecting excellent individuals from the offsprings through the enclosed breeding of the core groups, and obtaining the new strain of the polyembryonic cashmere goats. The present breeding method can effectively improve reproductive rates of cashmere goats, reduce production costs of female cashmere goats, improve breeding economic efficiency of cashmere goats, and can reduce the breeding number of female cashmere goats, save feeding grains, and speed up the industrialization process of cashmere goats.

The invention discloses a method for detecting goat CTNNB1 gene insertion/deletion and an application thereof. The method comprises the steps that a to-be-detected goat whole genome DNA is taken as atemplate, a goat CTNNB1 gene is augmented through a PLC technology, then agarose gel electrophoresis is performed, and it is identified whether or not the goat CTNNB1 gene has insertion/deletion sitepolymorphism. The correlation analysis results of a large sample (N)600) reveal that significant correlation exists between different types of CTNNB1 gene insertion/deletion and traits of the firstlambing of Shanbei white pile goats, and it is prompted that the different types of CTNNB1 gene insertion/deletion can be taken as a DNA mark improving the traits of the firstlambing of Shanbei white pile goats. The method for detecting goat CTNNB1 gene insertion/deletion can be used in mark-assisted selection breeding of reproduction traits of Chinese goats, and is conductive to quickly building goat genetic resource groups with a trait of high-yield goat number.

The present invention discloses a feeding method for increasing the wool of a down producing goat, which combines barn feeding and grazing. The top of a barn feeding down producing goat shed adopts more than three layers of black sunshade nets until the inside of the shed is completely dark; the time from May 15th to October 15th of each year is a wool growth period of sunlight limiting, during the period, the time from 30 minutes around 9:30 am to 30 minutes around 16:30 pm of each day is the time of free gauzing, feeding and drinking water for the down producing goat; the down producing goat is driven into the shed from 30 minutes around 16:30 pm to 30 minutes around 9:30 am in next day, and then the shed door is closed, which is the time of sunlight limiting; the down producing goat is additionally fed each once in the morning and in the evening in the shed. The feeding method has the advantages of the pressure of grazing grassland relieving, the ecology protecting, green and environmental protection, low cost and wool output improving. As the invention dose not use such growth promoting agent for wool as the drug, the additive and the hormone, etc., the invention has not any influence to the normal growth and production performance of the down producing goat.

The invention belongs to the field of cell culture, and in particular relates to a separation and identification method of dermal papilla cells of cashmere goats. The specific technical scheme is thatSOX<2+> and/or FGF<7+> and/or APOD<+> and/or HHIP<+> are/is used as marker genes of dermal papilla cells. The invention provides a method capable of accurately separating and identifying dermal papilla cells of cashmere goats for the first time. In terms of separation, an enzyme digestion method and a mechanical separation method are combined to jointly obtain cells; and furthermore, a culture medium special for culturing the dermal papilla cells of the cashmere goats is provided, so that required cells are rapidly obtained. In terms of identification, several new marker genes are jointly used for the first time in the invention, so that the required cells can be rapidly and accurately identified.

A cashmere goat lamb fattening pellet feed comprises the following components in weight percent: 10 weight percent of concentrated feed, 20 weight percent of corn seeds, 25-30 weight percent of corn stalk, 15-25 weight percent of sunflower seed husk, and 15-25 weight percent of vinasse. The feed is prepared into pellet feed. The cashmere goat fattening pellet feed is prepared from agricultural byproducts used as the raw materials on basis of balanced nutrition, so that the value in use of the agricultural byproducts can be increased, the waste materials are recycled, and the production cost of the feed can be greatly reduced, meanwhile, great improvement on fattening degree of cashmere goat is achieved, the weight increasing effect is obvious, and the economic income of farmers can be effectively increased.

The invention discloses a molecular marker influencing the cashmere length character of cashmere goats and application of the molecular marker. The site of the molecular marker comprises G>A mutationcorresponding to the 19532160 position on the No.2 chromosome of the goat genome ARS1 version. By the molecular marker, cashmere goats with relatively long cashmere can be rapidly and efficientlyscreened out, the cashmere quality and yield can be improved, genetic improvement can be rapidly and efficiently implemented for the cashmere goats when the molecular marker is applied to a breeding technology, and thus the market competitiveness of cashmere goat breed is improved.

The invention discloses a construction method for a DCN gene overexpression vector of a cashmere goat. The method comprises the following steps: (1) cloning a DCN gene; (2) constructing the overexpression vector pEGFP-Cl/DCN. According to the method, on the basis of cloning a DCN gene cDNA sequence of the cashmere goat, the overexpression vector pEGFP-Cl/DCN is constructed, and foundation is laid for follow-up research of DCN gene functions of the cashmere goat.

The invention discloses an extraction method for high-quality total RNA from the secondary follicle tissue of a Cashmere goat, which relates to the field of molecular biology. The extraction method comprises the following main steps: acquisition, preservation and transport of the secondary follicle tissue; separation of the secondary follicle tissue; extraction of total RNA of the secondary follicle tissue; and detection of quality of a total RNA sample. The method is simple to operate, has good stability and is suitable for common laboratory operation. The extracted total RNA of the secondary follicle tissue has high purity and good integrity and can be further applied to scientific research in related fields like preparation of cDNA, cloning of a target gene, real time fluorescent quantitative PCR detection and high flux sequencing. The method is also applicable to extraction of total RNA from follicle tissue of other animals.

The invention provides a genetic marker related to goat cashmere fiber diameter, the genetic marker is located on a KRTAP2-1 gene and has three dominant alleles A, B and C, and the nucleotide sequences of the alleles A, B and C are respectively SEQ ID No. 1-SEQ ID No. 3 in a sequence table; the cashmere fiber diameter of the individual containing the allele C is minimum; and the cashmere fiber diameter of the individual without the allele C is the largest. The genetic marker disclosed by the invention can be used for identifying the cashmere fiber diameter of the Longdong cashmere goats, and can be used for selecting the Longdong cashmere goats with small cashmere fiber diameters and eliminating the Longdong cashmere goats with large cashmere fiber diameters.

The invention provides a wild ibex and Xinjiang cashmere goat crossbreeding method. The method comprises the following steps: (1) selecting an ibex as a male parent and a Xinjiang cashmere goat as a female parent, performing prolificacy gene detection of the female parent, and performing artificial insemination to obtain first-filial generation F1; (2) carrying out backcrossing on the first-filial generation F1 obtained in the step (1) with the ibex male parent to obtain a second-filial generation F2, and performing prolificacy gene detection; (3) carrying out backcrossing on the second-filial generation F2 obtained in the step (2) with the Xinjiang cashmere goat female parent to obtain a third-filial generation F3, and performing prolificacy gene detection; and (4) selecting high-quality goat from the third-filial generation F3 obtained in the step (3), and performing crossing fixation to obtain a high-generation hybrid. After the hybridization and improvement of the method, Xinjiang ibex integrates multiple advantages of wild ibex and Xinjiang cashmere goat.