208 results about "Associated organism" patented technology

The invention belongs to the technical field of bioengineering, and particularly discloses a chimeric enzyme for degrading PET (Polyethylene Terephthalate) as well as a related biological material and application of the chimeric enzyme. The chimeric enzyme for degrading PET provided by the invention is obtained by replacing at least one fragment in an amino acid sequence as shown in SEQ ID No.1-12 on the basis of IsPETase. Compared with IsPETase, the chimeric enzyme for degrading PET provided by the invention has the advantages that the thermal melting temperature is increased by 4.8-31.7 DEG C, the concentration of a degradation product TPA after PET degradation is increased by 1.5-44.5 times, while the application conditions of PET degradation are widened, the PET degradation efficiency can be greatly improved, the recycling rate of the degradation product TPA with high added value is increased, and the chimeric enzyme has important economic value and environmental benefit. The method can be used in the fields of PET degradation, PET degradation agent preparation or PET degradation product preparation and the like.

The invention discloses cytosine deaminase as well as a related biological material and application thereof, and belongs to the technical field of protein. The technical problem to be solved by the invention is to excavate natural cytidine deaminase without sequence preference and improve the base editing efficiency and range. The invention provides a 15th-143rd site protein of which the amino acid sequence is SEQ ID NO.2. The invention also provides a preparation method of the protein. The invention also provides application of the protein and related biological materials thereof in single base editing. According to the application, metagenome big data is utilized, comprehensive and systematic information mining analysis is carried out on novel cytidine deaminase, and a protein L85 which is highly similar to a known DddA protein structure but low in sequence similarity is selected from the novel cytidine deaminase for functional verification. The protein L85 has high catalytic activity as cytidine deaminase, is not limited by target sequence preference, can be widely applied to gene editing in vitro and in cells / tissues, and has good application potential and development value.

The invention belongs to the technical field of biological prevention and control, and particularly relates to a Burkholderia paragallinarum D15 and application of the Burkholderia paragallinarum D15. The Burkholderia parakholderia sp. D15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 25, 2024, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 1.61962. The Burkholderia parakholderia sp. D15 has the advantages that the Burkholderia parakholderia sp. According to the invention, a multifunctional strain Burkholderia paragallinarum D15 is separated from the rock wall of Danxia red rock, and the Burkholderia paragallinarum D15 has a relatively good antagonistic effect on most plant pathogens and has a relatively strong bacteriostatic effect on xanthomonas oryzae Xoo causing rice bacterial blight; the compound also has a relatively strong bacteriostatic effect on other pathogenic bacteria causing crop diseases, such as rice sheath blight disease, rice blast, fusarium graminearum, sclerotium rolfsii and ralstonia solanacearum, and lays a foundation for prevention and treatment of rice bacterial blight and development of related biological preparations.

The invention discloses application of protein derived from wheat and related biological materials thereof in dwarf wheat breeding. The TaWAK3-B gene in a wheat genome is knocked out by utilizing a gene editing technology, so that a dwarf wheat variety is cultivated, and a novel molecular breeding method is provided for improving the yield and quality of wheat. Experimental results show that compared with wild type wheat, after TaWAK3-B is knocked out, the plant height of the knocked-out line wheat is remarkably reduced, and the heading period is remarkably delayed. Therefore, the TaWAK3-B protein has the potential of regulating the plant height and the growth and development period of wheat, and can be applied to lodging-resistant breeding and plant type improvement of plants.

The invention relates to the technical field of genetic engineering biology, and particularly discloses a GLP-1 analogue fusion protein as well as a preparation method and application thereof. According to the application, fGLP-1 and a mutant thereof are respectively fused with FSA, IgG4-Fc and a mutant thereof through connecting peptides to form a GLP-1 analogue fusion protein with the effects of high biological activity, long half-life period, high stability and the like, and meanwhile, after pichia pastoris codon is optimized and modified, an efficient pichia pastoris methanol-induced secretory expression system is utilized for expression; the problems of short half-life period, low biological activity, poor thermal stability, potential immunogenicity, complex production process, high purification and preparation cost and the like of the GLP-1 analogue prepared by the existing expression system and related biotechnology are solved.

The present invention discloses a design method and system for a pumping station heat dissipation system based on the Bio-TRIZ bionic theory. First, the improvement energy and conflicts existing in the pumping station heat dissipation structure are determined, a conflict matrix is established, and the Bio-TRIZ specific solution is determined. At the same time, in combination with the Bio-TRIZ biological example library, the biological specific solution corresponding to the Bio-TRIZ specific solution is matched, and relevant biological specific solutions are found through the biological example library to support this bionic design. Then, verification is carried out from aspects such as similar functions, similar structures, and similar boundary conditions, and the biological example with the highest bionic value is selected. Finally, the biological specific solution is analyzed, modeled by using the method of geometric feature reconstruction, and the bionic model is applied to the optimization of the pumping station heat dissipation device to improve the heat dissipation efficiency of the pumping station.

The invention discloses an OsUBC46 protein related to plant disease resistance and yield increase as well as a related biological material and application of the OsUBC46 protein. The OsUBC46 protein can be specifically a protein of A1), A2) or A3) as follows: A1) a protein of which the amino acid sequence is SEQ ID No.2 in a sequence table; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein of A1), has 75% or more of identity with the protein of A1) and has the activity of regulating and controlling plant disease resistance and yield increase; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The OsUBC46 protein and related biological materials thereof can be used for regulating and controlling the disease resistance and yield increase of plants.

The invention provides application of polypeptide or related biological materials thereof in serving and / or preparing products for regulating oral micro-ecological balance. The polypeptide with the amino acid sequence as shown in SEQ ID NO: 1 or related biological materials thereof can selectively inhibit harmful bacteria (such as streptococcus mutans) in the oral cavity, and also can ensure normal physiological activities of probiotics (such as streptococcus sanguis, streptococcus salivarius, streptococcus gordoni and the like) in the oral cavity, so that the regulation of micro-ecological balance of the oral cavity is realized, and the oral health of a human body is ensured.

A broad-spectrum disease resistance-related protein derived from wheat and related biomaterials and use thereof are provided. The protein is selected from the group consisting of A1) a protein with the amino acid sequence of SEQ ID NO: 1; A2) a protein having more than 80% identity to, having a same function as the protein with the amino acid sequence of SEQ ID NO: 1, and obtained by substitutions and / or deletions and / or additions of amino acid residues thereof; and A3) a fusion protein with a same function obtained by joining a tag at N-terminus and / or C-terminus of A1) or A2). The TaCNGC5.1 overexpression lines showed significantly improved disease resistance, thus suggesting that TaCNGC5.1 may play an important role in breeding plants with improved resistance to stripe rust.

The invention provides a deletion strain and a supplementation strain of related genes of Aeromonas dakara PhoBR and QSS as well as a construction method and application of the deletion strain and the supplementation strain, a deltaahyR, deltaluxS and deltaqseB gene deletion strain and a supplementation strain as well as a deltaphoB deltaahyR, deltaphoB deltaluxS and deltaphoB deltaqseB double-gene deletion strain are successfully constructed, it is proved that the PhoBR can directly regulate and control the related genes ahyR, luxS and qseB of the QSS in the Aeromonas dakara, negative regulation and control on the ahyR and the luxS and positive regulation and control on the qseB are shown, the QSS related genes can be directly regulated and controlled in the Aeromonas The regulation and control are crucial to the survival and environment adaptation of bacteria. Besides, through a related bioinformatics method, possible binding domains of part of PhoB and three QSS related genes ahyR, luxS and qseB have been predicted, and a scientific basis is further provided for immune prevention and treatment of Aeromonas dakara.

The invention provides high-stability recombinant I-type collagen and application thereof, and belongs to the technical field of collagen. The invention aims to avoid potential restriction enzyme cutting sites and unstable sequence fragments during sequence design through directional selection of the existing amino acid sequence, so that the amino acid sequence can be efficiently expressed in pichia pastoris, and the recombinant I-type collagen obtained after purification has very high stability; the recombinant I-type collagen gives consideration to factors such as related biological active sites, hydrophilicity and the like, has related biological activity while being relatively high in stability, and has very good practicability.

The invention discloses a protein SeCYP710A. The protein SeCYP710A is a protein shown as A1), A2) or A3): A1) a protein with an amino acid sequence shown as SEQ ID No.2; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence shown in A1), has the same function, is derived from A1) or has 80% or more of identity with the protein shown in A1) and has the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The invention also discloses a protein SeCYP710A related biological material and application thereof in improving the salt tolerance of plants. The protein SeCYP710A related biological material can be used for regulating and controlling the salt tolerance of the plants.

The invention discloses application of RNA (Ribonucleic Acid) binding protein CsdA in regulation and control of secondary metabolites of fungi. The invention provides application of RNA (Ribonucleic Acid) binding protein CsdA or related biological materials thereof in regulation and control of fungus secondary metabolites. The RNA binding protein CsdA is a protein of which the amino acid sequence is as shown in SEQ ID No. 3 or SEQ ID No. 2 or a homologous protein of the protein of which the amino acid sequence is as shown in SEQ ID No. 3 or SEQ ID No. 2. The invention is helpful for excavating natural products with medicinal activity in fungi, is helpful for developing new anti-fungal infection drug targets, and reduces the probability of fungal infection.

The application discloses cytosine deaminase and related biological materials and applications thereof, and belongs to the technical field of proteins. The technical problem to be solved by the application is to mine natural sequence-unpreferred cytosine deaminase, and to improve base editing efficiency and range. The application provides a protein with amino acid sequence of SEQ ID NO. 2 at positions 15-143. The application also provides application of the above protein and related biological materials thereof in single base editing. The application uses macro-genome big data, performs comprehensive and systematic bioinformatics mining analysis on novel cytosine deaminase, and selects a protein L85 highly similar to known DddA protein structure but with low sequence similarity for function verification. The protein L85 has high catalytic activity as cytosine deaminase, and has no target sequence preference restriction, and can be widely applied to in-vitro and in-cell / tissue gene editing, and has good application potential and development value.

The application belongs to the technical field of enzyme engineering, and specifically discloses a KL-CCF-MHETase mutant and related biological materials and applications thereof. The amino acid sequence of the KL-CCF-MHETase mutant provided by the application is shown in SEQ ID No. 3. Compared with the KL-MHETase mutant, the BHET degradation activity of the KL-CCF-MHETase mutant is significantly improved, and the KL-CCF-MHETase mutant can rapidly convert BHET into a target product TPA. Meanwhile, the application provides a PET degradation method. After an intermediate is prepared by chemical depolymerization of PET, the intermediate is combined with the KL-MHETase mutant for efficient conversion, and under the strategy of a crude substrate-crude enzyme, efficient degradation of high-crystallinity PET and green recovery of TPA are achieved. The application can be applied to the fields of degrading PET or BHET, preparing a degrading agent for PET or BHET, and recycling TPA.

The present invention discloses a circular gRNA and its related biomaterials and applications. The coding gene of the circular gRNA includes a 3' intron, a 3' splice site, a gRNA coding gene, a 5' splice site and a 5' intron in sequence, wherein the 3' splice site sequence is as shown in positions 2810-2814 of SEQ ID No.1, and the 5' splice site sequence is as shown in positions 2917-2930 of SEQ ID No.1. The circular gRNA is not attacked by nucleases, thereby improving the stability of the CRISPR / cas9 system.

The application discloses protein TaANT1-4D and application of biological materials related to the protein in regulation of ear type traits of plants, and aims to solve the technical problem of how to regulate the ear type traits of plants.The amino acid sequence of the protein TaANT1-4D provided by the application is shown in sequence 2 in the sequence listing.Experiments prove that knocking out TaANT1-4D in a wheat variety Fielder can significantly shorten the ear length, reduce the number of spikelets, the grain length and the thousand-grain weight.Therefore, the protein TaANT1-4D has important application value in regulation of the ear type traits of wheat and has a broad prospect in cultivation of wheat varieties.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

Provided are a Cinnamomum burmannii monoterpene synthase CbTPS1, an amino acid sequence thereof, a nucleic acid molecule encoding the protein, an use thereof in preparing the monoterpene synthase, and a method for preparing dextrorotatory borneol by using the Cinnamomum burmannii monoterpene synthase CbTPS1.

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

The invention discloses a gene CsANS1 for coding anthocyanin synthetase and application of the gene CsANS1, and belongs to the technical field of biology. The nucleotide sequence of the gene CsANS1 is as shown in SEQ ID NO. 1. The gene CsANS1 or related biological materials thereof can be used for synthesizing anthocyanin and quercetin in vitro. Recombinant engineering bacteria verify that the anthocyanin synthetase CsANS1 not only has activity of catalyzing colorless anthocyanin to generate anthocyanin, but also has relatively strong activity of catalyzing quercetin to generate, so that the limitation of anthocyanin and quercetin production in the prior art is effectively solved. The bifunctional catalytic characteristic of the gene CsANS1 widens the application prospect of ANS enzyme in metabolic engineering, and is helpful for promoting the development and utilization of natural active ingredients in the fields of food, medicine, cosmetics and the like.

The invention provides a qPCR (quantitative polymerase chain reaction) primer probe group for detecting various nodaviruses, a detection method and application, and belongs to the technical field of molecular biological detection. Aiming at various nodaviruses capable of infecting insect cell lines, including animal shed virus, Tn5 cell line virus, black beetle virus, borawa virus, nodavirus and macrobrachium rosenbergii nodavirus, a set of primer probe combination is designed. In the combination, part of viruses share the upstream primer and the probe, so that synchronous detection and identification of multiple target viruses in one reaction system are realized. The invention also provides a kit containing the primer probe group and a detection method. The detection method provided by the invention has the advantages of wide coverage range, high detection sensitivity, strong specificity, good matrix interference resistance and the like, and is suitable for safety detection and quality control of nodavirus pollution in insect cell banks, cell cultures and related biological products thereof.

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

Disclosed are novel methods and pharmaceutical compositions for creating tunable, protein target-specific polypeptides, their use for adjustable regulation of the levels of one or more protein targets and associated biological functions thereof, and applications to highly selective detection and / or isolation of one or more protein targets.

The invention discloses an application of a triterpenoid cyclase gene in regulation and control of rice grain size. The invention provides application of OsOSC10 protein or related biological materials thereof in regulation and control of plant grain development (size and weight). The related biological material is a nucleic acid molecule capable of expressing the OsOSC10 protein or an expression cassette, a recombinant vector, a recombinant bacterium or a transgenic cell line containing the nucleic acid molecule; the OsOSC10 protein is a protein as shown in SEQ ID No.1 or a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, or a protein which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of homology in sequence and has the same function, or a fusion protein obtained after the N end and / or C end of the protein are / is connected with a label. The invention has important significance for cultivating high-yield plants, especially rice varieties.

The application discloses application of a PvFIP37 protein and related biological materials thereof in regulating plant cadmium tolerance. The application clones a PvFIP37 gene from switchgrass and successfully constructs a PvFIP37 overexpression switchgrass. Through analysis of a phenotype of the PvFIP37 overexpression switchgrass under cadmium stress, it is found that, compared with a wild type switchgrass, the increase of the PvFIP37 gene expression amount can enhance the cadmium tolerance of the switchgrass, which indicates that the PvFIP37 has a function of regulating plant cadmium tolerance. The switchgrass PvFIP37 gene provided by the application can be used as an excellent gene resource and is widely applied to the genetic breeding field of the gramineous grasses or other crops, and has important significance for understanding of the plant tolerance under heavy metal stress.

The application discloses a method for improving the functional expression level of DgePPK2, and first constructs a recombinant expression vector containing a codon-optimized DgePPK2 coding gene; the chaperone-assisted expression vector is selected from at least one of pGro7, pG-KJE8, pTF16 and pKJE7, the chaperone-assisted expression vector and the DgePPK2 recombinant expression vector are sequentially transformed into an E. coli host cell, a recombinant engineering strain is obtained, and chaperone and DgePPK2 protein are co-expressed. The application effectively improves the soluble expression level of DgePPK2 by introducing a chaperone-assisted expression system, so that the crude enzyme obtained under the same culture condition has higher ATP regeneration capacity. The application can be applied to in-vitro enzyme catalytic reaction, multi-enzyme cascade reaction and related biological manufacturing processes requiring continuous supply of ATP, and has good application value.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The application discloses C4 corn ZmClpP6 protein and application of related biological materials thereof in regulation of high light response capability of plants. ZmClpP6 It is found that: ZmClpP6 The knockout can reduce chlorophyll content, total carotenoid content, catalase activity and copper-zinc SOD enzyme activity of corn under high light conditions, and makes corn leaves appear yellowing phenotype. It is indicated that ZmClpP6 can regulate high light response capability of plants. The application clones ZmClpP6 the gene in corn for the first time and analyzes the biological function thereof, and has important significance for cultivation of high light efficiency plants.

The invention discloses a tylosin sensing biosensor, a construction method thereof and MphR mutant protein based on the tylosin sensing biosensor, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to detect tylosin. In order to solve the technical problem, the invention provides the biosensor for sensing tylosin, the biosensor can express an MphR31D1 protein and / or a coding gene containing the MphR31D1 protein, and the MphR31D1 protein can be a protein with an amino acid sequence of SEQ ID NO: 1. The invention also provides a preparation method of the biosensor, and application of the MphR31D1 protein and related biological materials thereof in detection of tylosin. According to the invention, escherichia coli Top10 is taken as a chassis bacterium, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, and the tylosin whole-cell biosensor is successfully constructed through protein directed evolution.