155 results about "Associated organism" patented technology

Isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for transporting sugars across cell membranes using isolated and / or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

A method and apparatus to measure and analyze electromagnetic emissions from implanted electronics to accomplish at least one of: detect and identify an active implant comprising electronics implanted in a biological host, diagnose the health of individual electronics as well as their health as an ensemble, and predict probable degradation of individual electronics, degradation of the electronic ensemble, and of the active implant in an automated fashion. The methods comprise filtering techniques to extract the information about the active implant's electronics by filtering out relevant biological effects induced by the active implant's biological situation. Said detection, identification, diagnosis, and prognosis are based on the measurement of intentional and unintentional electromagnetic emissions that emanate from the implant electronics of the active implant in a given biological situation.

The present invention provides a nanostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the substrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures.

Methods and apparatus for identifying disease status according to various aspects of the present invention include analyzing the levels of one or more biomarkers. The methods and apparatus may use biomarker data for a condition-positive cohort and a condition-negative cohort and select multiple relevant biomarkers from the plurality of biomarkers. The system may generate a statistical model for determining the disease status according to differences between the biomarker data for the relevant biomarkers of the respective cohorts. The methods and apparatus may also facilitate ascertaining the disease status of an individual by producing a composite score for an individual patient and comparing the patient's composite score to one or more threshold for identifying potential disease status.

The invention discloses a set of adenine base editors as well as related biological materials and application thereof. The invention provides an application of fusion protein in plant single base editing. The fusion protein has a name of TadA-R-cas and contains Cas protein and adenine deaminase, and the adenine deaminase is protein located at the 1st-167th sites and having an amino acid sequence as shown in SEQ ID No.2. The fusion protein is not only suitable for the adenine base editor containing SpCas9, but also suitable for the adenine base editor containing ScCas9, SpCas9-NG and SpRY, theapplication range of plant genome fixed-point editing is widened, and an important gene function research tool is provided for researchers in the field of plant research. The fusion protein can improve the editing efficiency and accurately mediate the mutation of a target site, and can be widely applied to rice cells.

The invention discloses a cultivation method of genetically modified WMYB-R wheat resistant to root rot and sheath blight and a related biological material thereof. The cultivation method includes the step of guiding coding gene of WMYB-R protein into a receptor plant to obtain a genetically modified plant with disease resistance higher than that of the receptor plant. If the WMYB-R gene is modified into plants, resistance, of the plants, to root rot and sheath blight can be improved, so that pesticide consumption is lowered to reduce environmental pollution; the cultivation method has important theoretical and practical significance and can play an important role in genetic improvement of the plants.

The invention discloses a method for evaluating the influence of to-be-tested objects (an anti-fatigue drug, an anti-fatigue and a pollutant) on the exercise tolerance of an organic body and special equipment of the method. The method comprises the following steps: (1) correcting the flow speed of a system water body; (2) screening an experimental fish shoal; (3) testing the swimming resistance; and (4) collecting experimental data, and analyzing a result. The special equipment comprises a water tank, a water pump, a container for swimming of fishes, a flow regulation valve, a flow meter, a nitrogen bottle, an oxygen bottle, a refrigerator and a high-speed camera. The method can be used for carrying out pre-warning judgment on an abnormal environmental water body, testing a drug, evaluating the exercise tolerance of different species of small-sized fishes in different water body environments, evaluating and testing the a nutritious supplementary and the like. The equipment can be automatically assembled and occupies a small area, parameters such as the temperature, the water flow speed and the dissolved oxygen amount can be controlled, and the equipment is easy and convenient to operate, relatively low in manufacturing cost and convenient for the application of relevant biological experiments.

This invention provides novel biomarkers for Niemann-Pick disease, type C (NPC). In an exemplary embodiment, the invention provides methods for identifying a subject as having NPC. In embodiments, the methods involve detecting the level of biomarker selected from the group comprising i) galectin-3 (LGALS3); ii) cathepsin D (CTSD); iii) LGALS3 and CTSD; and iv) LGALS3 and / or CTSD in combination at least one additional NPC associated biomarker in a sample obtained from the subject. In embodiments, the methods involve comparing the level of biomarker to a reference.

The invention discloses triterpene-2-alpha-hydroxylase MAA45, related biological materials thereof and application of the triterpene two-bit alpha-hydroxylase MAA45 and the related biological materials in preparing maslinic acid and corosolic acid. The triterpene two-bit alpha-hydroxylase MAA45 is the following protein shown as A1), or A2) or A3), wherein A1) represents protein with an amino acid sequence being the sequence 1 in a sequence table, A2) represents protein which has the same function and is obtained by substituting or missing or adding one or more amino acid residues to the amino acid sequence shown in the sequence 1, and A3) represents fused protein obtained by connecting labels to the N terminal or / and C terminal of A1) or A2). It is proved through experiments that maslinic acid can be prepared from MAA45 with oleanolic acid as a substrate, corosolic acid can be prepared from MAA45 with ursonic acid as a substrate, MAA45 and coding genes thereof can be used for preparing maslinic acid and corosolic acid, and MAA45 can be used for catalyzing triterpene two-bit alpha-hydroxylation.

The invention discloses disease resistance associated protein TaCPK7-R derived from wheat and related biological materials and application thereof. The TaCPK7-R is the protein of a) or b), wherein the amino acid sequence is the protein of sequence 2; and the amino acid sequence shown by the sequence 2 in the sequence table is substituted by one or more amino acid residues and / or deleted and / or is add with the protein related to the disease resistance of plants. Experiments prove that TaCPK7-R and biological materials related to the TaCPK7-R can be used for regulating and controlling the disease resistance of plants, and particularly can be used for regulating and controlling the resistance of wheat to banded sclerotial blight.

The present application relates to methods and systems of identifying molecules or processes of biological interest by using knowledge discovery in biological data. In particular, the present application describes new methods of creating a biological map, new methods of codifying such map, new methods of analyzing such map and new methods of identifying molecules and processes of biological interest. The present application provides methods and systems to identify new and useful direct or indirect therapeutic targets, molecular modulators, adverse events effectors, disease biomarkers, genetic biomarkers, safety-related biomarkers, diagnostic molecules, hormones, metabolites, or metabolic effectors of any type.

The invention discloses a method and a related biological material for cultivating a transgenic plant of fluorescent protein mCherry labeled microtubulin. The method comprises the step of importing PTUB6:mCherry-TUB6 to a receiver plant to obtain the transgenic plant of the fluorescent protein mCherry labeled microtubulin, wherein PTUB6:mCherry-TUB6 is formed by connecting mCherry-TUB6 fusion genes with PTUB6 for starting transcription of the mCherry-TUB6 fusion genes, the mCherry-TUB6 fusion genes are genes for encoding fusion protein mCherry-TUB6, the fusion protein mCherry-TUB6 is protein obtained by fusing fluorescent protein mCherry with plant microtubulin, and PTUB6 is a DNA molecule shown in 1st-3004th positions of a nucleotide sequence shown in SEQ ID No.1.

The invention discloses application of a protein and related biological materials thereof to regulation and control on mechanical strength of plants. The invention firstly discloses application of a BCL1 protein and related biological materials thereof to regulation and control on mechanical strength of plants. The invention further discloses a method for cultivating transgenic plants of which stalks and / or leaves have increased brittleness. The invention not only provides the theoretical basis for cultivating fragile stalk and fragile leaf plant materials and rice varieties of which cell wallcomponents are easy to degrade and which is high in recycling efficiency, but also provides a new gene resource for rice breeding.

An artificially synthesized insect-resistant protein, biological materials associated therewith, and use thereof are disclosed. The protein is designated as MRP001 protein, and is a protein of A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No.6; and A2) a protein having insect resistance derived from A1) by substituting, and / or deleting, and / or adding one or more amino acid residues in the amino acid sequence of the protein of A1). It is confirmed through experiments that the MRP001 protein and biological materials associated therewith have resistance to plant bug, and are useful in the preparation of biological ant-insect agent containing the protein, and in the cultivation of insect-resistant transgenic cotton, fruits, tea, rice, vegetables and other crops, thereby reducing the application of pesticides and alleviating the environmental pollution, and thus being of great economic value and broad application prospect.

The invention discloses a method for cultivating a transgenic gramineous plant with increased tiller number and a biological material related with the method. The method comprises the following steps: guiding a DNA (Deoxyribonucleic Acid) molecule PGH3-IPT into a receptor-gramineous plant to obtain the transgenic gramineous plant with increased tiller number, increased spike number and / or reduced plant height in comparison with the receptor-gramineous plant. Compared with the receptor-gramineous plant, the transgenic gramineous plant obtained by guiding the DNA molecule PGH3-IPT into the receptor-gramineous plant has the characteristics that malformation caused by excess change of plant morphology can not be caused when the effective tiller number and plant height of the transgenic gramineous plant are properly reduced; the spike number and grain weight of the transgenic gramineous plant are not remarkably changed, so that the yield of a single gramineous plant is increased; other morphology and growth and development characteristics of the plant are not remarkably changed.

The invention discloses a disease resistance-related protein RCR1 derived from wheat, the related biomaterials thereof, and an application for the same. RCR1 is the protein a) or the protein b) as follows: a) the protein composed of amino acid sequences shown in a sequence 2 in a sequence table; and b) the protein obtained by performing the substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequences shown in the sequence 2 in the sequence table, and related to plant disease resistance. Experiments proved that RCR1 and the biomaterials relates to RCR1 can be used for regulating plant disease resistance, and especially used for regulating the resistance of wheat to banded sclerotial blight.

The invention discloses an HPV18 E and E7 fusion gene mutant as well as a related biological material and coding protein thereof. The HPV18 E and E7 fusion gene mutant disclosed by the invention is DNA molecule represented as a) or b): a) coding sequence is DNA molecule (named as HPV18-E6E7-4 gene) represented on 15th-805th sites of SEQ ID No. 1; b) coding sequence is DNA molecule (named as HPV18-E6E7-3 gene) represented on 15th-806th sites of SEQ ID No. 2. The HPV18-E6E7-4 gene or HPV18-E6E7-3 gene not only has high expression on mammalian cell but also is free from oncogenicity; and the fusion gene mutant has biological safety, and is capable of effectively preventing and treating cervical cancer.

The present invention relates to a method for non-destructively identifying a characteristic of a Gallus Gallus domesticus embryo in ovo, the method comprising: (a) obtaining a sample of material associated with an egg comprising the embryo, and (b) measuring a score value for the presence of, and concentration of at least a first biomarker in the sample indicative of the characteristic of the embryo, and (c) applying a threshold to the score value and concentration obtained in (b) to identify the characteristic for the embryo associated with the presence and concentration of the biomarker, wherein an at least first biomarker comprises an amino compound having a molecular weight in the range of from 140 to 190 g / mole, wherein step (c) further comprises: (i) correlating each relevant biomarker signal with a reference biomarker by matching the spectrum of each correlating signal with the expected spectrum of the correlating reference biomarker using a similarity measure, to define at least one positively correlating signal; (ii) measuring the intensity of each positively correlating signal and scoring its absolute and / or relative signal intensity; and (iii) applying a threshold to the score value obtained from a similarity function to determine the correlated embryo characteristic.

The invention discloses a disease-resistance gap-associated protein TaPK-R1 derived from wheat as well as a related biological material and an application thereof. The TaPK-R1 is a protein (a) formed by amino acid sequences as shown in a sequence 2 in a sequence table or protein (b) related to plant disease resistance and formed by the amino acid sequences as shown in the sequence 2 in the sequence table, which are replaced and / or deleted and / or added by one or several amino acid residues. The experiments prove that the TaPK-R1 and the biological material related to the TaPK-R1 can be used for adjusting and controlling plant disease resistance, and particularly can be used for adjusting and controlling the banded sclerotial blight resistance of wheat.

The invention discloses alpha-L-fucosidase and a related biological material and application thereof. The invention discloses a protein which is A1) a protein of which the amino acid sequence is shownin the formula SEQ ID No.4, A2) a protein of which the amino acid sequence is shown in the formula of SEQ ID No.3, A3) a fusion protein obtained by connecting the N end or / and C end of the protein ofA1) or A2) with a protein label, and A4) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the protein shown in the formula of SEQ ID No.3 or SEQ ID No.4, has 90% or more of identity with the protein shown in A1) or A2) and has the same function as the protein shown in A1) or A2). The invention further discloses a related biological material of the protein and an application of the related biological material. The protein provided by the invention can be used for efficiently synthesizing 3'-fucosyllactose and has a good application prospect in oligosaccharide synthesis.

The invention discloses application of a protein and a related biological material thereof in increasing plant yield. The protein is the following A1) or A2) or A3): A1) protein of which the amino acid sequence is Sequence 2; A2) plant-yield-related protein obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence disclosed as Sequence 2 in the sequence table; or A3) fusion protein obtained by connecting a tag to the N terminal or / and C terminal of A1) or A2). The increase of the plant yield comprises improvement of plant yield related traits, including at least one of spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number. The experiment proves that the protein and coding gene thereof can be utilized to enhance the spike length, number of spike grains, weight of thousand-grain, grain length, grain width, primary rachis branch number and secondary rachis branch number of the plants, thereby increasing the plant yield.

The invention relates to a xylanase intestinal directional expression vector and a cell line thereof and belongs to the biotechnical field. An XynB gene is inserted into an eukaryotic expression vector pcDNA3.1(-), a lentivirus recombinant vector Lanti4-blockit-RELMbeta-XynB-myc-GFP is constructed, and primary culture porcine embryonic fibroblasts are transfected to construct an eukaryocyte line of intestinal directional expression XynB zymoprotein. Therefore, a necessary transgenic biomaterial and a key technical system support can be provided for further cloning of trans-XynB gene embryo by somatic cell nuclear transplantation, and breeding of a new transgenic swine variety with high feed utilization and low pollutant discharge as well as the field of associated biological scientific research and production.

The invention provides a related biomarker for diagnosing and treating oral squamous cell carcinoma and application thereof, and particularly relates to a biomarker RP11-426C 22.5. The expression level of the biomarker RP11-426C22.5 is detected, and the biomarker RP11-426C22.5 can be used for diagnosing the oral squamous cell carcinoma and can be targeted to be used for treating the oral squamouscell carcinoma.

The invention belongs to the field of microorganism animal cell lines, and relates to a cell system for generating hepatitis B virus (HBV) recombinant cccDNA. Through a transposon system, HBV single-copy genomes with two ends provided with loxP sites are integrated into HepG2 cells, a clone HepG2-HBV / loxP cell line integrated with the different-copy-number HBV genomes is obtained, the cell line does not express HBsAg, after cyclization recombination enzyme (Cre) is introduced through adenovirus transduction, the rcccDNA is generated and the HBsAg is expressed, and expression of the HBsAg is closely relevant to the generation and level of the rcccDNA; the rcccDNA is rapidly and stably generated, the structure feature of the rcccDNA is similar to that of real HBV cccDNA, and transcription, copy and protein expression of viruses can be supported; the rcccDNA can be quantitatively detected through quantitative PCR by designing a specific primer, and Southern Blot detection can be carried out by utilizing a digoxin marking system. The cell system can be used for establishing a platform for HBV cccDNA relevant biological research and screening and evaluation of anti-HBV medicines.

The invention discloses application of a protein TabZIP60 in regulation of plant root system development. The invention provides the application of the TabZIP60 protein or related biological materialsthereof in regulation of plant root system development. The related biological materials are nucleic acid molecules capable of expressing the TabZIP60 protein, or expression cassettes, recombinant vectors, recombinant bacteria or transgenic cell lines, which contain the nucleic acid molecules. In the aspect of regulating plant root system development through the TabZIP60 protein, compared with wild type plants, the dry weight of root systems and / or the total lateral root length of transgenic plants in which TabZIP60 is over expressed is decreased obviously, and the dry weight of root systemsand / or the total lateral root length of transgenic plants in which TabZIP60 is less expressed is increased obviously. Therefore, the protein TabZIP60 can be utilized for regulating plant root system development. The application of the protein TabZIP60 in regulation of plant root system development has important application value to selective breeding of high-yield plant novel materials.

A method and apparatus to measure and analyze electromagnetic emissions from implanted electronics to accomplish at least one of: detect and identify an active implant comprising electronics implanted in a biological host, diagnose the health of individual electronics as well as their health as an ensemble, and predict probable degradation of individual electronics, degradation of the electronic ensemble, and of the active implant in an automated fashion. The methods comprise filtering techniques to extract the information about the active implant's electronics by filtering out relevant biological effects induced by the active implant's biological situation. Said detection, identification, diagnosis, and prognosis are based on the measurement of intentional and unintentional electromagnetic emissions that emanate from the implant electronics of the active implant in a given biological situation.

The invention discloses miR156 and application of a related biomaterial thereof in regulating disease resistance of plants. Transgenic miR156 white birch is used as a research object, wild type whitebirch serves as a control, the activity of superoxide dismutase, catalase, peroxidase, polyphenol oxidase and phenylalanine ammonialyase is determined by detecting the speck number after diseases occur, and the effects of miR156 on damage degree of disease infected plants is researched in the physiological aspect; by analyzing the transgenic miR156 white birch and disease-resistant related genes in the control, regulation of miR156 on disease resistant ways of plants is further proved. The miR156 has great significance in disclosing disease resistant functions of the miR156 and breeding disease-resistant white birch varieties and contributes to enrichment of forest tree breeding resources.

The invention discloses ZmAER protein and a coding gene and application thereof. The invention claims ZmAER protein or application its related biomaterial in regulating plant tolerance to low nitrogen. Olefine aldehyde oxidoreductase coded via corn ZmAER gene, provided herein, can regulate the content of active carbonyl compounds to affect the tolerance to low nitrogen; a new idea can be provided for studying nitrogen-efficient mechanisms of crops; therefore, a new strategy is provided for the cultivation of nitrogen-efficient varieties.

Methods and apparatus for identifying disease status according to various aspects of the present invention include analyzing the levels of one or more biomarkers. The methods and apparatus may use biomarker data for a condition-positive cohort and a condition-negative cohort and select multiple relevant biomarkers from the plurality of biomarkers. The system may generate a statistical model for determining the disease status according to differences between the biomarker data for the relevant biomarkers of the respective cohorts. The methods and apparatus may also facilitate ascertaining the disease status of an individual by producing a composite score for an individual patient and comparing the patient's composite score to one or more thresholds for identifying potential disease status.