123 results about "Associated organism" patented technology

The invention discloses application of protein derived from wheat and related biological materials thereof in dwarf wheat breeding. The TaWAK3-B gene in a wheat genome is knocked out by utilizing a gene editing technology, so that a dwarf wheat variety is cultivated, and a novel molecular breeding method is provided for improving the yield and quality of wheat. Experimental results show that compared with wild type wheat, after TaWAK3-B is knocked out, the plant height of the knocked-out line wheat is remarkably reduced, and the heading period is remarkably delayed. Therefore, the TaWAK3-B protein has the potential of regulating the plant height and the growth and development period of wheat, and can be applied to lodging-resistant breeding and plant type improvement of plants.

The invention provides a deletion strain and a supplementation strain of related genes of Aeromonas dakara PhoBR and QSS as well as a construction method and application of the deletion strain and the supplementation strain, a deltaahyR, deltaluxS and deltaqseB gene deletion strain and a supplementation strain as well as a deltaphoB deltaahyR, deltaphoB deltaluxS and deltaphoB deltaqseB double-gene deletion strain are successfully constructed, it is proved that the PhoBR can directly regulate and control the related genes ahyR, luxS and qseB of the QSS in the Aeromonas dakara, negative regulation and control on the ahyR and the luxS and positive regulation and control on the qseB are shown, the QSS related genes can be directly regulated and controlled in the Aeromonas The regulation and control are crucial to the survival and environment adaptation of bacteria. Besides, through a related bioinformatics method, possible binding domains of part of PhoB and three QSS related genes ahyR, luxS and qseB have been predicted, and a scientific basis is further provided for immune prevention and treatment of Aeromonas dakara.

The application discloses cytosine deaminase and related biological materials and applications thereof, and belongs to the technical field of proteins. The technical problem to be solved by the application is to mine natural sequence-unpreferred cytosine deaminase, and to improve base editing efficiency and range. The application provides a protein with amino acid sequence of SEQ ID NO. 2 at positions 15-143. The application also provides application of the above protein and related biological materials thereof in single base editing. The application uses macro-genome big data, performs comprehensive and systematic bioinformatics mining analysis on novel cytosine deaminase, and selects a protein L85 highly similar to known DddA protein structure but with low sequence similarity for function verification. The protein L85 has high catalytic activity as cytosine deaminase, and has no target sequence preference restriction, and can be widely applied to in-vitro and in-cell / tissue gene editing, and has good application potential and development value.

The application belongs to the technical field of enzyme engineering, and specifically discloses a KL-CCF-MHETase mutant and related biological materials and applications thereof. The amino acid sequence of the KL-CCF-MHETase mutant provided by the application is shown in SEQ ID No. 3. Compared with the KL-MHETase mutant, the BHET degradation activity of the KL-CCF-MHETase mutant is significantly improved, and the KL-CCF-MHETase mutant can rapidly convert BHET into a target product TPA. Meanwhile, the application provides a PET degradation method. After an intermediate is prepared by chemical depolymerization of PET, the intermediate is combined with the KL-MHETase mutant for efficient conversion, and under the strategy of a crude substrate-crude enzyme, efficient degradation of high-crystallinity PET and green recovery of TPA are achieved. The application can be applied to the fields of degrading PET or BHET, preparing a degrading agent for PET or BHET, and recycling TPA.

The present invention discloses a circular gRNA and its related biomaterials and applications. The coding gene of the circular gRNA includes a 3' intron, a 3' splice site, a gRNA coding gene, a 5' splice site and a 5' intron in sequence, wherein the 3' splice site sequence is as shown in positions 2810-2814 of SEQ ID No.1, and the 5' splice site sequence is as shown in positions 2917-2930 of SEQ ID No.1. The circular gRNA is not attacked by nucleases, thereby improving the stability of the CRISPR / cas9 system.

The application discloses protein TaANT1-4D and application of biological materials related to the protein in regulation of ear type traits of plants, and aims to solve the technical problem of how to regulate the ear type traits of plants.The amino acid sequence of the protein TaANT1-4D provided by the application is shown in sequence 2 in the sequence listing.Experiments prove that knocking out TaANT1-4D in a wheat variety Fielder can significantly shorten the ear length, reduce the number of spikelets, the grain length and the thousand-grain weight.Therefore, the protein TaANT1-4D has important application value in regulation of the ear type traits of wheat and has a broad prospect in cultivation of wheat varieties.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

Provided are a Cinnamomum burmannii monoterpene synthase CbTPS1, an amino acid sequence thereof, a nucleic acid molecule encoding the protein, an use thereof in preparing the monoterpene synthase, and a method for preparing dextrorotatory borneol by using the Cinnamomum burmannii monoterpene synthase CbTPS1.

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

The invention discloses a gene CsANS1 for coding anthocyanin synthetase and application of the gene CsANS1, and belongs to the technical field of biology. The nucleotide sequence of the gene CsANS1 is as shown in SEQ ID NO. 1. The gene CsANS1 or related biological materials thereof can be used for synthesizing anthocyanin and quercetin in vitro. Recombinant engineering bacteria verify that the anthocyanin synthetase CsANS1 not only has activity of catalyzing colorless anthocyanin to generate anthocyanin, but also has relatively strong activity of catalyzing quercetin to generate, so that the limitation of anthocyanin and quercetin production in the prior art is effectively solved. The bifunctional catalytic characteristic of the gene CsANS1 widens the application prospect of ANS enzyme in metabolic engineering, and is helpful for promoting the development and utilization of natural active ingredients in the fields of food, medicine, cosmetics and the like.

The invention provides a qPCR (quantitative polymerase chain reaction) primer probe group for detecting various nodaviruses, a detection method and application, and belongs to the technical field of molecular biological detection. Aiming at various nodaviruses capable of infecting insect cell lines, including animal shed virus, Tn5 cell line virus, black beetle virus, borawa virus, nodavirus and macrobrachium rosenbergii nodavirus, a set of primer probe combination is designed. In the combination, part of viruses share the upstream primer and the probe, so that synchronous detection and identification of multiple target viruses in one reaction system are realized. The invention also provides a kit containing the primer probe group and a detection method. The detection method provided by the invention has the advantages of wide coverage range, high detection sensitivity, strong specificity, good matrix interference resistance and the like, and is suitable for safety detection and quality control of nodavirus pollution in insect cell banks, cell cultures and related biological products thereof.

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

Disclosed are novel methods and pharmaceutical compositions for creating tunable, protein target-specific polypeptides, their use for adjustable regulation of the levels of one or more protein targets and associated biological functions thereof, and applications to highly selective detection and / or isolation of one or more protein targets.

The application discloses application of a PvFIP37 protein and related biological materials thereof in regulating plant cadmium tolerance. The application clones a PvFIP37 gene from switchgrass and successfully constructs a PvFIP37 overexpression switchgrass. Through analysis of a phenotype of the PvFIP37 overexpression switchgrass under cadmium stress, it is found that, compared with a wild type switchgrass, the increase of the PvFIP37 gene expression amount can enhance the cadmium tolerance of the switchgrass, which indicates that the PvFIP37 has a function of regulating plant cadmium tolerance. The switchgrass PvFIP37 gene provided by the application can be used as an excellent gene resource and is widely applied to the genetic breeding field of the gramineous grasses or other crops, and has important significance for understanding of the plant tolerance under heavy metal stress.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The application discloses C4 corn ZmClpP6 protein and application of related biological materials thereof in regulation of high light response capability of plants. ZmClpP6 It is found that: ZmClpP6 The knockout can reduce chlorophyll content, total carotenoid content, catalase activity and copper-zinc SOD enzyme activity of corn under high light conditions, and makes corn leaves appear yellowing phenotype. It is indicated that ZmClpP6 can regulate high light response capability of plants. The application clones ZmClpP6 the gene in corn for the first time and analyzes the biological function thereof, and has important significance for cultivation of high light efficiency plants.

The invention discloses a tylosin sensing biosensor, a construction method thereof and MphR mutant protein based on the tylosin sensing biosensor, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to detect tylosin. In order to solve the technical problem, the invention provides the biosensor for sensing tylosin, the biosensor can express an MphR31D1 protein and / or a coding gene containing the MphR31D1 protein, and the MphR31D1 protein can be a protein with an amino acid sequence of SEQ ID NO: 1. The invention also provides a preparation method of the biosensor, and application of the MphR31D1 protein and related biological materials thereof in detection of tylosin. According to the invention, escherichia coli Top10 is taken as a chassis bacterium, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, and the tylosin whole-cell biosensor is successfully constructed through protein directed evolution.

Provided are a high-stability recombinant type I collagen and a use thereof, relating to the technical field of collagens. By means of the directional selection of existing amino acid sequences, potential enzyme cutting sites and unstable sequence fragments are avoided during sequence design, enabling efficient expression in Pichia pastoris; and in addition, after being purified, the recombinant type I collagen still has high stability. The recombinant type I collagen takes into account factors such as related biological active sites and hydrophilicity, has related biological activity while maintaining high stability, and has good practicability.

The application discloses application of a protein and related biomaterials thereof in regulating soybean plant type and improving yield. The application belongs to the technical field of biotechnology, and particularly relates to application of the protein and related biomaterials thereof in regulating soybean plant type and improving yield. The protein of the application is a composition of proteins with amino acid sequences of SEQ ID No. 1 and / or SEQ ID No. 4; or a protein with a function of regulating plant type or / and yield, which is obtained by substitution, deletion and / or addition of amino acid residues on the above-mentioned protein; or a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the above-mentioned protein. By regulating the activity or content of the above-mentioned protein, a soybean variety with changed plant type and improved yield can be obtained.

This invention relates to the field of biotechnology, and discloses the protein IpSTP5, which is related to the oil content of *Vernicia fordii* fruit, and its related biomaterials and applications. Specifically, the IpSTP5 protein can be one of the following: A1), A2), or A3): A1) a protein whose amino acid sequence is SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substituting and / or deleting and / or adding one or more amino acid residues to the protein of A1), possessing more than 90% identity and the same activity as the protein shown in A1); A3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of A1) or A2). The IpSTP5 protein and its related biomaterials can be used to positively regulate the oil content of *Vernicia fordii* fruit.

The invention discloses application of insect COX5B protein or related biological materials thereof in preparation of products for resisting black-streaked dwarf virus infection and products for controlling insects to spread black-streaked dwarf viruses. The invention also discloses an application of the specific antibody of the insect COX5B protein in detection of black-streaked dwarf virus in an insect body. The invention also discloses a method for controlling transmission of southern rice black-streaked dwarf virus by sogatella furcifera. It is found that the sogatella furcifera COX5B protein can inhibit acquisition and copying of SRBSDV, so that accumulation of the SRBSDV is inhibited, host survival is enhanced, the COX5B protein can inhibit propagation of the SRBSDV in sogatella furcifera, and the COX5B protein can be used for preparing products for resisting black-streaked dwarf virus infection and products for controlling propagation of the black-streaked dwarf virus by insects so as to improve the black-streaked dwarf virus resistance of crops; the kit can also be used for detecting black-streaked dwarf viruses in insect bodies.

The invention belongs to the field of molecular biology, and particularly relates to a protein Tasg-D3 and application of related biological materials of the protein Tasg-D3. The Tasg-D3 is a protein of which the amino acid sequence is as shown in SEQ ID NO: 3. According to the invention, a gene for coding Tasg-D3 is introduced into wheat Fielder to obtain Tasg-D3 overexpression strains OE7, OE13 and OE17, and the plant height and grain length of the Tasg-D3 overexpression strains are obviously lower than those of a receptor control Fielder, which indicates that the Tasg-D3 and the coding gene thereof negatively regulate the plant height and grain weight of a plant. The Tasg-D3 and related biological materials thereof can be used for cultivating lodging-resistant varieties, and the wheat breeding process is accelerated.

The invention belongs to the technical field of fluorescent materials and biomedical engineering, and relates to a Zn-DPA complex as well as a preparation method and application thereof. Zn-DPA complexes are zinc-bipyridine methylamine (Zn-DPA) complexes with different alkyl chain lengths, the fluorescent dye disclosed by the invention is combined with the zinc-bipyridine methylamine (Zn-DPA) complexes with different alkyl chain lengths, so that the targeting property of gram bacteria can be regulated and controlled, and a preparation prepared from the fluorescent dye has NIR-II gram bacteria selectivity; the fluorescent probe has high fluorescence signal-to-noise ratio, good photothermal conversion efficiency and biocompatibility, can be used for selective imaging and photothermal therapy of gram bacterial infection, and has potential application value in prevention and treatment of drug-resistant bacterial infection and related biomedical research.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The application provides a virus envelope chimeric receptor and related biomaterials and applications thereof, and belongs to the technical field of molecular biology. Specifically disclosed is a virus envelope chimeric receptor, which comprises, in sequence, an antibody or antigen-binding fragment thereof, a hinge region, a transmembrane region and an intracellular region; the hinge region is selected from a hinge region composed of a hinge region of a CD8 molecule and a hinge region of a vesicular stomatitis virus envelope glycoprotein; the transmembrane region of the virus envelope chimeric receptor is selected from a transmembrane region of the vesicular stomatitis virus envelope glycoprotein; and the intracellular region of the virus envelope chimeric receptor is selected from an intracellular region of the vesicular stomatitis virus envelope glycoprotein. The virus envelope chimeric receptor is used for virus targeting, can improve the infection ability of viruses, reduces the infection rate of viruses on non-T cells to less than 5%, provides a safety guarantee for in-vivo CAR-T preparation, and can be used for industrial production.

The invention discloses a wheat grain weight gene WGY1 as well as an encoded protein and application thereof, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to regulate and control the grain weight of wheat. To this end, the present application provides proteins and related biomaterials that modulate plant yield. The invention also provides application of the protein and related biological materials thereof in regulation and control of plant yield. According to the application, the WGY1 gene is finally obtained through gene localization and map-based cloning. The gene and the protein coded by the gene can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and play an important role in improving germplasm resources of crops such as wheat and the like.

This invention belongs to the fields of biomedicine and molecular biology, focusing on the application of NAT10 and related biomolecules in the diagnosis and treatment of deep vein thrombosis (DVT). Specifically, this invention has found that NAT10 is a key initiator of endothelial cell ferroptosis, and its expression is significantly increased in DVT. The results indicate that inhibiting NAT10 reduces ac4C levels in DVT, thereby alleviating endothelial ferroptosis. However, decreased NAT10 expression leads to reduced HMOX1 stability and decreased Fe... 2+ The release and inhibition of lipid peroxidation make targeting the NAT10 / HMOX1 axis a potential treatment for DVT, and therefore this invention has good potential practical application value.

The invention discloses a related protein GmPP2C338 for regulating and controlling the salt tolerance of a plant as well as a related biological material and application of the related protein GmPP2C338. And the protein sequence of the soybean protein GmPP2C338 is as shown in a sequence 2. The technical problem to be solved by the invention is how to improve the salt tolerance of plants. The invention specifically discloses a protein, and an encoding gene of the protein is applied to the following applications: A1) application of a product for improving the salt tolerance of plants; a2) plant breeding and / or preparation of plant breeding products. The salt tolerance of a plant of which the expression of the GmPP2C338 protein coding gene is knocked out / reduced is obviously higher than that of a control plant. The substance for inhibiting or reducing or down-regulating the expression of the GmPP2C338 gene can be used for improving the salt tolerance of plants and can be used for plant breeding.

This invention discloses a gene CsANS1 encoding anthocyanin synthase and its applications, belonging to the field of biotechnology. The nucleotide sequence of the gene CsANS1 is shown in SEQ ID NO.1. The gene CsANS1 or related biological materials can be used to synthesize anthocyanins and quercetin in vitro. This invention verifies through recombinant engineered bacteria that the anthocyanin synthase CsANS1 not only has the activity of catalyzing the conversion of colorless anthocyanins to anthocyanins, but also has a strong catalytic activity of catalyzing the conversion of quercetin, effectively overcoming the limitations of existing technologies in the production of anthocyanins and quercetin. The bifunctional catalytic properties of the gene CsANS1 broaden the application prospects of ANS enzymes in metabolic engineering and help promote the development and utilization of natural active ingredients in the fields of food, medicine, and cosmetics.

The application provides application of LcSFC6 protein and related biological materials thereof. The amino acid sequence of the LcSFC6 protein is shown as SEQ ID No: 2. The application takes wild Leymus chinensis as a receptor material to obtain LcSFC6 The overexpression plant of the gene. Compared with the wild type, LcSFC6 The stress resistance of the overexpression plant is significantly improved. The LcSFC6 and the coding gene thereof have important theoretical and practical significance for cultivating new varieties of Leymus chinensis and other plants with improved stress resistance, and can be applied to cultivation and identification of plant varieties with resistance required by agriculture, animal husbandry and ecological environment management, and have high practical application value. The application has wide application prospect in the field of agriculture.