78 results about "Associated organism" patented technology

The application belongs to the technical field of enzyme engineering, and specifically discloses a KL-CCF-MHETase mutant and related biological materials and applications thereof. The amino acid sequence of the KL-CCF-MHETase mutant provided by the application is shown in SEQ ID No. 3. Compared with the KL-MHETase mutant, the BHET degradation activity of the KL-CCF-MHETase mutant is significantly improved, and the KL-CCF-MHETase mutant can rapidly convert BHET into a target product TPA. Meanwhile, the application provides a PET degradation method. After an intermediate is prepared by chemical depolymerization of PET, the intermediate is combined with the KL-MHETase mutant for efficient conversion, and under the strategy of a crude substrate-crude enzyme, efficient degradation of high-crystallinity PET and green recovery of TPA are achieved. The application can be applied to the fields of degrading PET or BHET, preparing a degrading agent for PET or BHET, and recycling TPA.

The application discloses protein TaANT1-4D and application of biological materials related to the protein in regulation of ear type traits of plants, and aims to solve the technical problem of how to regulate the ear type traits of plants.The amino acid sequence of the protein TaANT1-4D provided by the application is shown in sequence 2 in the sequence listing.Experiments prove that knocking out TaANT1-4D in a wheat variety Fielder can significantly shorten the ear length, reduce the number of spikelets, the grain length and the thousand-grain weight.Therefore, the protein TaANT1-4D has important application value in regulation of the ear type traits of wheat and has a broad prospect in cultivation of wheat varieties.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

Provided are a Cinnamomum burmannii monoterpene synthase CbTPS1, an amino acid sequence thereof, a nucleic acid molecule encoding the protein, an use thereof in preparing the monoterpene synthase, and a method for preparing dextrorotatory borneol by using the Cinnamomum burmannii monoterpene synthase CbTPS1.

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

The invention provides a qPCR (quantitative polymerase chain reaction) primer probe group for detecting various nodaviruses, a detection method and application, and belongs to the technical field of molecular biological detection. Aiming at various nodaviruses capable of infecting insect cell lines, including animal shed virus, Tn5 cell line virus, black beetle virus, borawa virus, nodavirus and macrobrachium rosenbergii nodavirus, a set of primer probe combination is designed. In the combination, part of viruses share the upstream primer and the probe, so that synchronous detection and identification of multiple target viruses in one reaction system are realized. The invention also provides a kit containing the primer probe group and a detection method. The detection method provided by the invention has the advantages of wide coverage range, high detection sensitivity, strong specificity, good matrix interference resistance and the like, and is suitable for safety detection and quality control of nodavirus pollution in insect cell banks, cell cultures and related biological products thereof.

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

Disclosed are novel methods and pharmaceutical compositions for creating tunable, protein target-specific polypeptides, their use for adjustable regulation of the levels of one or more protein targets and associated biological functions thereof, and applications to highly selective detection and / or isolation of one or more protein targets.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The application discloses C4 corn ZmClpP6 protein and application of related biological materials thereof in regulation of high light response capability of plants. ZmClpP6 It is found that: ZmClpP6 The knockout can reduce chlorophyll content, total carotenoid content, catalase activity and copper-zinc SOD enzyme activity of corn under high light conditions, and makes corn leaves appear yellowing phenotype. It is indicated that ZmClpP6 can regulate high light response capability of plants. The application clones ZmClpP6 the gene in corn for the first time and analyzes the biological function thereof, and has important significance for cultivation of high light efficiency plants.

Provided are a high-stability recombinant type I collagen and a use thereof, relating to the technical field of collagens. By means of the directional selection of existing amino acid sequences, potential enzyme cutting sites and unstable sequence fragments are avoided during sequence design, enabling efficient expression in Pichia pastoris; and in addition, after being purified, the recombinant type I collagen still has high stability. The recombinant type I collagen takes into account factors such as related biological active sites and hydrophilicity, has related biological activity while maintaining high stability, and has good practicability.

The application discloses application of a protein and related biomaterials thereof in regulating soybean plant type and improving yield. The application belongs to the technical field of biotechnology, and particularly relates to application of the protein and related biomaterials thereof in regulating soybean plant type and improving yield. The protein of the application is a composition of proteins with amino acid sequences of SEQ ID No. 1 and / or SEQ ID No. 4; or a protein with a function of regulating plant type or / and yield, which is obtained by substitution, deletion and / or addition of amino acid residues on the above-mentioned protein; or a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the above-mentioned protein. By regulating the activity or content of the above-mentioned protein, a soybean variety with changed plant type and improved yield can be obtained.

The invention belongs to the technical field of fluorescent materials and biomedical engineering, and relates to a Zn-DPA complex as well as a preparation method and application thereof. Zn-DPA complexes are zinc-bipyridine methylamine (Zn-DPA) complexes with different alkyl chain lengths, the fluorescent dye disclosed by the invention is combined with the zinc-bipyridine methylamine (Zn-DPA) complexes with different alkyl chain lengths, so that the targeting property of gram bacteria can be regulated and controlled, and a preparation prepared from the fluorescent dye has NIR-II gram bacteria selectivity; the fluorescent probe has high fluorescence signal-to-noise ratio, good photothermal conversion efficiency and biocompatibility, can be used for selective imaging and photothermal therapy of gram bacterial infection, and has potential application value in prevention and treatment of drug-resistant bacterial infection and related biomedical research.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The application provides a virus envelope chimeric receptor and related biomaterials and applications thereof, and belongs to the technical field of molecular biology. Specifically disclosed is a virus envelope chimeric receptor, which comprises, in sequence, an antibody or antigen-binding fragment thereof, a hinge region, a transmembrane region and an intracellular region; the hinge region is selected from a hinge region composed of a hinge region of a CD8 molecule and a hinge region of a vesicular stomatitis virus envelope glycoprotein; the transmembrane region of the virus envelope chimeric receptor is selected from a transmembrane region of the vesicular stomatitis virus envelope glycoprotein; and the intracellular region of the virus envelope chimeric receptor is selected from an intracellular region of the vesicular stomatitis virus envelope glycoprotein. The virus envelope chimeric receptor is used for virus targeting, can improve the infection ability of viruses, reduces the infection rate of viruses on non-T cells to less than 5%, provides a safety guarantee for in-vivo CAR-T preparation, and can be used for industrial production.

This invention belongs to the fields of biomedicine and molecular biology, focusing on the application of NAT10 and related biomolecules in the diagnosis and treatment of deep vein thrombosis (DVT). Specifically, this invention has found that NAT10 is a key initiator of endothelial cell ferroptosis, and its expression is significantly increased in DVT. The results indicate that inhibiting NAT10 reduces ac4C levels in DVT, thereby alleviating endothelial ferroptosis. However, decreased NAT10 expression leads to reduced HMOX1 stability and decreased Fe... 2+ The release and inhibition of lipid peroxidation make targeting the NAT10 / HMOX1 axis a potential treatment for DVT, and therefore this invention has good potential practical application value.

The invention discloses a related protein GmPP2C338 for regulating and controlling the salt tolerance of a plant as well as a related biological material and application of the related protein GmPP2C338. And the protein sequence of the soybean protein GmPP2C338 is as shown in a sequence 2. The technical problem to be solved by the invention is how to improve the salt tolerance of plants. The invention specifically discloses a protein, and an encoding gene of the protein is applied to the following applications: A1) application of a product for improving the salt tolerance of plants; a2) plant breeding and / or preparation of plant breeding products. The salt tolerance of a plant of which the expression of the GmPP2C338 protein coding gene is knocked out / reduced is obviously higher than that of a control plant. The substance for inhibiting or reducing or down-regulating the expression of the GmPP2C338 gene can be used for improving the salt tolerance of plants and can be used for plant breeding.

This invention discloses a gene CsANS1 encoding anthocyanin synthase and its applications, belonging to the field of biotechnology. The nucleotide sequence of the gene CsANS1 is shown in SEQ ID NO.1. The gene CsANS1 or related biological materials can be used to synthesize anthocyanins and quercetin in vitro. This invention verifies through recombinant engineered bacteria that the anthocyanin synthase CsANS1 not only has the activity of catalyzing the conversion of colorless anthocyanins to anthocyanins, but also has a strong catalytic activity of catalyzing the conversion of quercetin, effectively overcoming the limitations of existing technologies in the production of anthocyanins and quercetin. The bifunctional catalytic properties of the gene CsANS1 broaden the application prospects of ANS enzymes in metabolic engineering and help promote the development and utilization of natural active ingredients in the fields of food, medicine, and cosmetics.

The application provides application of LcSFC6 protein and related biological materials thereof. The amino acid sequence of the LcSFC6 protein is shown as SEQ ID No: 2. The application takes wild Leymus chinensis as a receptor material to obtain LcSFC6 The overexpression plant of the gene. Compared with the wild type, LcSFC6 The stress resistance of the overexpression plant is significantly improved. The LcSFC6 and the coding gene thereof have important theoretical and practical significance for cultivating new varieties of Leymus chinensis and other plants with improved stress resistance, and can be applied to cultivation and identification of plant varieties with resistance required by agriculture, animal husbandry and ecological environment management, and have high practical application value. The application has wide application prospect in the field of agriculture.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of alfalfa genes and related biomaterials. The applications include: (A1) improving plant cold resistance; (A2) increasing plant leaf area; and (A3) promoting seed germination. The alfalfa gene is either the MsCKX5 gene or the MsCKX15 gene. The nucleotide sequence of the MsCKX5 gene is shown in SEQ ID NO:1; the nucleotide sequence of the MsCKX15 gene is shown in SEQ ID NO:2. The application is achieved by overexpressing the MsCKX5 gene or the MsCKX15 gene in plants. This provides important molecular evidence for alfalfa genetic improvement.

The application discloses application of ZmHB77 protein and a coding gene thereof in regulation of drought resistance of plants. The application discloses application of ZmHB77 protein (SEQ ID No. 1) or a related biological material thereof in all or part of the following: regulation of drought resistance of plants, regulation of seed root number of plants, regulation of lateral root density of plants, and the related biological material is a nucleic acid molecule capable of expressing the ZmHB77 protein or an expression cassette containing the nucleic acid molecule, a recombinant vector, a recombinant bacterium or a transgenic cell line. After gene knockout of the ZmHB77 gene drought resistance protein coding gene of the application, the drought resistance of corn is significantly improved. The application provides a more precise, efficient and safe technical method for creating a corn overexpression plant and / or corn breeding, realizes precise improvement of the drought resistance of corn, can promote the commercial corn breeding process, overcomes the short board of traditional breeding, shortens the breeding cycle and does not affect other properties.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

This invention provides a primer-probe set, detection method, and application for qPCR detection of various nodaviruses, belonging to the field of molecular biology detection technology. This invention designs a primer-probe combination for various nodaviruses that can infect insect cell lines, including farm virus, Tn5 cell line virus, black beetle virus, Boralwa virus, Nodamura virus, and Macrobrachium rosenbergii nodavirus. In this combination, some viruses share upstream primers and probes, enabling simultaneous detection and identification of multiple target viruses in a single reaction system. This invention also provides a kit and detection method containing this primer-probe set. The detection method of this invention has advantages such as wide coverage, high detection sensitivity, strong specificity, and good resistance to matrix interference, and is suitable for the safety detection and quality control of nodavirus contamination in insect cell banks, cell cultures, and related biological products.

The application discloses ZmECT2 protein and application of an encoding gene thereof in regulating maize kernel quality. The application provides the following application of ZmECT2 protein or related biological materials (nucleic acid molecules expressing ZmECT2 protein or expression cassettes containing the nucleic acid molecules, recombinant vectors, recombinant microorganisms or transgenic cell lines): regulating plant kernel quality, regulating plant kernel protein content and / or starch content; the ZmECT2 protein is SEQ ID No. 1 or a protein with more than 80% identity compared with the ZmECT2 protein and having the same function and being derived from rice after substitution, deletion and / or addition of one or more amino acids or after connection of a tag to the N terminal and / or C terminal of the ZmECT2 protein. The ZmECT2 in the application can regulate plant kernel quality, including regulating kernel protein content and starch content. The application has important application value for effectively regulating plant kernel quality by means of genetic breeding and genetic engineering.

The application discloses a multiplex qPCR primer probe set, a kit, a detection method and application for detecting alphaviruses, and belongs to the technical field of molecular biological detection. The application designs a set of primer probe combination for a plurality of alphaviruses capable of infecting insect cell lines, including western equine encephalitis virus, eastern equine encephalitis virus, sindbis virus, chikungunya virus, AURA virus and Venezuelan equine encephalitis virus. The combination realizes synchronous detection and identification of a plurality of target viruses in one reaction system. The application also provides a kit containing the primer probe set and a corresponding detection method. The detection method has the advantages of wide coverage, high detection sensitivity, strong specificity, good anti-matrix interference capability and the like, and is suitable for safety detection and quality control of alphavirus contamination in insect cell banks, cell cultures and related biological products.

The invention discloses truncated high-activity cytosine deaminase as well as a related biological material and application thereof, and belongs to the technical field of protein. In order to improve the base editing efficiency and range, the invention provides a 15th-150th protein with an amino acid sequence of SEQ ID NO.2 and application of the 15th-150th protein as cytidine deaminase. The invention also provides application of the protein and related biological materials thereof in single base editing. According to the application, metagenome big data is utilized, comprehensive and systematic information mining analysis is carried out on novel cytidine deaminase, and a protein L91 which is highly similar to a known DddA protein structure but low in sequence similarity is selected from the novel cytidine deaminase for functional verification. The protein L91 has high catalytic activity as cytidine deaminase, is not limited by target sequence preference, can be widely applied to gene editing in vitro and in cells / tissues, and has good application potential and development value.

The invention provides a non-natural amino acid modified protein, a related biological material, a preparation method and application. The non-natural amino acid modified protein provided by the invention is obtained by modifying soluble BAFF variant protein by using p-nitrobenzene alanine; the modification site of the p-nitrobenzene alanine is located at least one of the 91st site, the 100th site, the 139th site and the 158th site of the soluble BAFF variant protein. According to the invention, non-natural amino acid is introduced to a specific site of soluble BAFF variant protein, so that the antigen structure of the protein is controllably changed, the immune tolerance of an organism to endogenous BAFF is effectively broken, the organism is induced to generate humoral immune response aiming at BAFF, the neutralization effect on the endogenous BAFF is realized, and the aim of improving the safety of the organism is fulfilled. And a new technical approach is provided for prevention and treatment of related autoimmune diseases.

The application discloses an epitope tandem polypeptide of porcine reproductive and respiratory syndrome and related biological materials and application thereof, and belongs to the field of viral antigen vaccines or biomedical technologies. The application provides an epitope tandem polypeptide which is composed of neutralizing antigen epitopes, Th epitopes and CTL epitopes of multiple structural proteins and non-structural proteins of PRRSV in series, and includes PNB, PTH and PCTL. The epitope tandem polypeptide provided by the application can be directly used for preparing a subunit vaccine of PRRSV, and has the advantages of simple preparation method, strong immunogenicity and good safety. The epitope tandem polypeptide can also be combined with an inactivated vaccine of PRRSV and applied to enhance cellular immunity induced by the inactivated vaccine, so that the epitope tandem polypeptide can more effectively prevent and protect pigs from PRRSV infection. The epitope tandem polypeptide composition contains common epitope sequences of seven representative strains (LV, VR-2332, CH-1a, JXA1, NADC30, NADC34 and RFLP 1-4-4) of PRRSV, and is expected to have good cross-protection for infection of different strains.

This invention discloses a strain of *Pleurotus ostreatus* and its application in the degradation of mycotoxins. The strain exhibits the ability to degrade various mycotoxins (including OTA, ZEN, AFB1, and DON). The freeze-dried fruiting body powder and mycelium powder extract of this strain show a degradation efficiency of up to 99% for OTA (1 μg / mL), and the mycelium extract achieves near-complete removal of OTA within 6 hours. The fruiting bodies of the *Pleurotus ostreatus* strain 914T1 of this invention are characterized by high water content and tender texture. Their dry matter is rich in total sugar, protein, and cellulose, expanding the scope of edible fungi in the field of mycotoxin degradation and providing new strain resources for the development of related biological agents.

The invention belongs to the technical field of biological medicine and molecular biology, and relates to application of NAT10 and related biomolecules thereof in diagnosis and treatment of deep vein thrombosis. Specifically, research finds that NAT10 is an important starting factor of endothelial cell ferroptosis, and the expression of NAT10 is remarkably increased in DVT. The result shows that the endothelial ferroptosis is relieved by inhibiting the NAT10 to reduce the ac4C level in the DVT. However, reduction of expression of NAT10 leads to reduction of stability of HMOX1, reduction of release of Fe < 2 + > and inhibition of lipid peroxidation, so that targeting NAT10 / HMOX1 axis may be a potential method for treating DVT, and the application has good potential practical application value.