3 results about "ATP regeneration" patented technology

This invention discloses a reaction tube for recombinase polymerase amplification (RPA), belonging to the field of nucleic acid detection. The reaction tube of this invention includes a tube body and a cap separate from the tube body. The tube body contains lyophilized bulbs or lyophilized powder containing premixed nucleic acid detection reagents, and the cap contains lyophilized powder containing an activator. The tube body and cap are sealed with a thin film. The lyophilized bulbs or lyophilized powder containing the premixed nucleic acid detection reagents include: recombinase, single-stranded DNA-binding protein, strand displacement DNA polymerase, buffer components, ATP, ATP regeneration components, and dNTPs. The lyophilized powder containing the activator includes: potassium ions, magnesium ions, and polyethylene glycol. This invention enables magnesium acetate to stably adhere to the cap, solving the problem of easy spillage of the lyophilized activator in the cap. In use, only the nucleic acid of the sample to be tested needs to be added; the operation is simple, easy to promote and apply, and suitable for batch detection.

The application provides a multi-dimensional information adaptive RNA cell-free synthesis system implementation method, comprising: obtaining a preset enzyme conformation adjustment parameter and a microenvironment pH regulation template according to a functional deviation degree, an activity offset amplitude and an ATP regeneration rate fluctuation of the ATP regenerating enzyme, generating an enzyme conformation dynamic regulation configuration, and determining a dynamic energy supply output mode matched with a current synthesis stage; based on real-time feedback after running of the dynamic energy supply output mode, collecting energy supply cycle frequency data, combining an intermediate product accumulation rate and an enzyme and stage adaptation index in the system, processing full-dimensional monitoring data of the energy feedback cycle, and judging an adaptation matching level of the ATP regenerating enzyme.

This invention belongs to the field of biotechnology and discloses a method for the continuous preparation of β-nicotinamide mononucleotide (NMN). The invention covalently immobilizes four functional enzymes—ribokinase, phosphoribosylpyrokinase, nicotinamide phosphoribosyltransferase, and polyphosphate kinase—on an alkali-activated epoxy resin microsphere carrier to prepare a multi-enzyme composite catalyst. Using D-ribose, nicotinamide, and low-polymerization-degree polyphosphate as substrates, a small amount of ATP-initiating reaction solution is used to carry out continuous catalysis in a packed bed reactor. Polyphosphate kinase enables in-situ, zero-distance ATP regeneration, highly coupling ATP regeneration with NMN synthesis, significantly improving energy utilization and cascade catalytic efficiency. This invention solves the problems of high ATP consumption, non-recoverable enzymes, difficulty in continuous production, and limited diffusion of intermediate products in traditional enzymatic methods. The process is stable, green, safe, and suitable for industrial scale-up.