50 results about "Acetyl phosphate" patented technology

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

The present invention discloses an in-vitro cell-free protein synthesis system. The system comprises cell extract, carbohydrate and a phosphate compound, wherein the carbohydrate is maltodextrin, lactose, or a combination of the maltodextrin and glucose, or a combination of the lactose and the glucose, or a combination of the maltodextrin, the lactose and the glucose. ATP is provided for an in-vitro reaction by low-cost substances such as the glucose, the maltodextrin and the lactose instead of energy sources such as phosphoenolpyruvic acid, phosphocreatine and acetyl phosphate, so that whilethe cost is reduced, a mode of providing energy by slow release prolongs the reaction time and increases the yield of target protein.

The invention relates to an improved preparation method of acetyl diammonium phosphate. According to the improved preparation method, phosphoric acid is reacted with acetic anhydride firstly so as to obtain acetyl phosphate, and then ammonium hydroxide is added so as to obtain acetyl diammonium phosphate. The improved preparation method is simple in operation, high in yield, and low in cost, and is especially suitable for industrialized production.

The invention discloses a method for preparing acetyl phosphate on the basis of biological enzymes. The method comprises the following steps: carrying out enzyme catalysis reaction on lactic acid and/or lactate and phosphoric acid and/or phosphate and/or biphosphate by using an immobilized oxidase lactate-immobilized pyruvate oxidase composite enzyme as a catalyst; and after the reaction finishes, filtering and precipitating to obtain the high-purity acetyl phosphate. The method is simple to operate, has the advantages of mild reaction conditions, high raw material conversion rate, low cost and environmental protection, and satisfies the industrial production.

The invention provides an enzymatic synthesis method of PAP. The enzymatic synthesis method comprises the following steps: generating PAP with 5'-adenosine monophosphate (AMP) as a substrate, 5'-phosphosulfated kinase (APSK) as a catalyst and 5'-adenosine triphosphate (ATP) or 5'-adenosine diphosphate (ADP) as a coenzyme; and synthesizing ATP with acetylphosphoric acid or polyphosphoric acid as aphosphate group donor and acetokinase (AK) or polyphosphoric kinase (PK) as a catalyst, thereby realizing ATP circulation (as shown in Figure 1). The method has the advantages that the PAP is synthesized in one step by taking AMP as a substrate, and that the theoretical utilization rate of phosphorus reaches 100%.

The invention relates to a kit for diagnosing/measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamic acid, adenyl pyrophosphate, acetyl phosphate, glutamine synthetase, acetokinase, aldehyde dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

The invention discloses a method for reducing the proportion of succinic acid fermentation by-products of actinobacillus succinogenes, comprising the following steps: carrying out fermentation cultureon strains subjected to strain activation and propagation, with the inoculum size of 5-15% by volume; and adding 1-20 g/L of hydrosulphite or metabisulfite into the fermentation medium. According tothe invention, hydrosulphite or metabisulfite is added in the process of producing succinic acid by fermentation of actinobacillus succinogenes, and is subjected to addition reaction with an intermediate product acetyl phosphate generated in cells to hinder further conversion into acetic acid, so that the content of acetic acid by-products can be greatly reduced under the condition of not influencing the yield of succinic acid. The process is simple in operation process and low in cost, and has industrial application value.

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.

The invention relates to an inorganic phosphorus (phosphate radical) diagnosis/determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, adenoside bisphosphate, oxaloacetic acid, acetyl phosphate, hydrogen peroxide, coenzyme A, pyruvate carboxylase, pyruvate oxidase, pyruvate dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV/ visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.

The invention relates to a monoamine oxidase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining monoamine oxidase activity concentration and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, reduced coenzyme, amines, sodium bicarbonate (carbon dioxide), acetyl phosphate, pyruvic oxidase, alanine dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV/ visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of the monoamine oxidase.

The invention discloses a method for continuous production of acetyl phosphate with a microchannel reaction device. The method includes the steps of: (1) pumping a phosphoric acid solution and an acetic anhydride solution respectively into a microstructure mixer I at the same time, mixing the substances evenly, then pumping the mixture into a microchannel reactor I and carrying out reaction to obtain an acetyl phosphate solution; (2) pumping the acetyl phosphate solution and an alkali solution respectively into a microstructure mixer II at the same time, and then pumping the mixture into a microchannel reactor II to carry out reaction to obtain an acetyl phosphate solution; and (3) conducting standing, crystallization, filtering and drying on the acetyl phosphate solution to obtain acetyl phosphate. The method provided by the invention can achieve a phosphoric acid conversion rate of 100%, and a acetyl phosphate yield of more than 93%.

A recombinant microorganism for producing 2,3-butanediol consisting of selecting at least three groups from uridine diphosphate glucose phosphate uroglycan transferase gene (galU), acetyl alcohol dehydrogenase gene (acoA), acetyl phosphate transferase gene (pta), adenosine glucosylphosphate transferase gene (glgC), lactose dehydrogenase gene (ldhA), and phosphodiesterase gene (pdeC) which were modified.

The invention relates to an inorganic phosphorus (phosphate radical) diagnostic and measuring kit utilizing technologies of an enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring inorganic phosphorus (phosphate radical) concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science, food and environment. The kit mainly comprises the following components: buffer solution, reduced coenzyme, oxaloacetic acid, acetyl phosphate, hydrogen peroxide, phosphoenolpyruvate carboxylase (PEPC), pyruvic oxidase, malate dehydrogenase and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV/visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the inorganic phosphorus (phosphate radical) concentration.