11 results about "Acetyl phosphate" patented technology

The invention relates to a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, and belongs to the technical field of biosynthesis. In order to solve the existing problem of serious environmental pollution, the invention provides a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, which comprises the following steps: in the presence of polyphosphate, magnesium salt, ATP (adenosine triphosphate), pyridoxal kinase and polyphosphate kinase, performing phosphorylation reaction on pyridoxal or pyridoxine and acid salts thereof, thereby obtaining the pyridoxal phosphate or pyridoxine phosphate. The temperature of the phosphorylation reaction is controlled to be 30-38 DEG C, after the reaction is finished, protein separation is conducted on reaction liquid, mother liquor is separated, the compound pyridoxal phosphate or pyridoxine phosphate shown in the formula I is obtained, and the mother liquor is recycled and reused. According to the method, a large amount of ammonium acetophosphate does not need to be additionally added, operation is simpler, mother liquor can be recycled, a large amount of waste water is prevented from being discharged, environment friendliness is better achieved, and the yield is high.

The invention belongs to the technical field of genetic engineering, and discloses a genetically engineered bacterium for efficiently synthesizing gamma-aminobutyric acid from the beginning by efficiently utilizing a mixed carbon source, a method and application, and the genetically engineered bacterium is obtained by overexpressing edd from Zymomonas mobilis on the basis of escherichia coli E. coli G16; eda from the zymomonas mobilis is over-expressed; performing overexpression on acs (Acetobacter pasteurianus) sources; a malic acid synthase gene aceB is knocked out; a malic acid synthase gene glcB is knocked out; and the acetyl phosphate transferase gene eutD is knocked out. The escherichia coli strain which is clear in genetic background and capable of producing gamma-aminobutyric acid is utilized, and an ED path and an acetic acid utilization path are introduced, so that acetyl CoA branch metabolism is reduced, and the yield and conversion rate of gamma-aminobutyric acid are remarkably improved.

The invention discloses a construction method and application of escherichia coli genetically engineered bacteria for producing malic acid by synergistically utilizing CO2, and belongs to the technical field of bioengineering. According to the invention, an L-malic acid chassis strain MG1655W (DE3) constructed by a gene editing technology in a laboratory is used as an initial strain, a lactic dehydrogenase encoding gene ldhA and a phosphate acetyltransferase encoding gene pta in escherichia coli are knocked out on the basis, the production paths of lactic acid and formic acid are blocked, and the yield of lactic acid and formic acid is increased. The method comprises the following steps of: firstly, amplifying a phosphoenolpyruvate carboxylase gene ppc and a CO2 transporter gene CA, constructing a recombinant plasmid, introducing the recombinant plasmid pACYCDuet-1-CA-ppc into an expression host MG1655W (DE3) delta ldhA delta pta, and screening to obtain the engineering bacterium MG1655W (DE3) delta ldhA delta pta-CA-ppc by overexpressing the ppc gene and the CA gene and improving the CO2 immobilization and concentration capability of escherichia coli. The obtained engineering bacterium is subjected to cell proliferation under an aerobic condition and malic acid accumulation under an oxygen limiting condition, the final malic acid yield is increased to 140-212%, and the engineering bacterium has important industrial application value.

This invention relates to the field of bioengineering, and more particularly to a high-chondroitin-producing engineered Bacillus subtilis strain, its construction method, and its applications. The invention provides an engineered strain using Bacillus subtilis as a substrate strain, expressing an enzyme comprising UDP-N-acetylglucosamine C4 isomerase, chondroitin synthase, and one or more of the following enzyme combinations: UDP-glucose dehydrogenase; UTP-glucose-1-phosphate uridine transferase; glutamine-fructose-6-phosphate aminotransferase; UDP-N-acetylglucosamine pyrophosphorylase / glucose-1-phosphate acetyltransferase; and phosphogluconomutase. This invention, through metabolic engineering strategies, designs and constructs a stable, plasmid-free, high-chondroitin-producing engineered strain, overcoming existing yield bottlenecks and achieving efficient, safe, and sustainable production of chondroitin sulfate, providing technical support for its widespread application in the food, health product, and pharmaceutical fields.

The invention relates to a method for increasing the yield of lactose-N-tetraose based on a dynamic regulation and control system, and belongs to the technical field of biological engineering. UDP-N-acetylglucosamine-2-epimerase, beta-galactosidase and 6-phosphofructokinase coding genes are knocked out from escherichia coli, glucosamine 6-phosphate synthase, N-acetyl glucosamine 1-phosphate uridinetransferase / glucosamine 1-phosphate acetyltransferase and glucosamine phosphomutase are expressed, and the glucosamine 6-phosphate synthase, the N-acetyl glucosamine 1-phosphate uridinetransferase and the glucosamine phosphomutase are respectively expressed in beta-1, 2, 3, 4, 5, 6-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, c ends of the beta-1, 3-N-acetylglucosamine transferase and the beta-1, 3-galactosyl transferase are connected with polypeptide tags, and a stable-phase promoter is used for expressing a micro-interval protein and a tag ligand, so that the polypeptide tags and the tag ligand are specifically combined. In order to further increase the yield of a target product, a growth-coupled promoter is used for expressing 6-phosphofructokinase and a degradation tag, uncoupling of cell growth and a product synthesis process is realized, the final yield of lactose-N-tetrasaccharide reaches 2.89 g / L, the production intensity is 0.04 g / L / h, and the method has relatively good application prospects and industrial potential.

The invention relates to phosphoketolase MaPKT and SzPKT with a function of catalyzing formaldehyde to synthesize acetyl phosphate and application of phosphoketolase MaPKT and SzPKT in CO2 biosynthesis of ethanol, and belongs to the technical field of enzyme mining and biological catalysis application. A phosphoketolase specific protein sequence database and an oligopeptide module sequence library are established through an oligopeptide module recognition (PPR) technology, two phosphoketolase PKTs capable of catalyzing formaldehyde to synthesize acetyl phosphate are efficiently and accurately excavated, further research finds that the formaldehyde tolerance of MaPKT is improved, and the catalytic efficiency of MaPKT on formaldehyde is 8 times that of glycolaldehyde. Based on this, the invention also constructs an efficient condensation approach for synthesizing ethanol by artificially biologically converting CO2, and has potential application prospects.

A genetically modified microorganism for increasing the production of a polyketide compound or a derivative thereof is provided, including any one or both of the following genetic modifications: (a) a deleted endogenous pta gene encoding a phosphate acetyltransferase, wherein an expression level of the phosphate acetyltransferase of the deleted endogenous pta gene is lower than an expression level of its wild type; and (b) an added first exogenous nucleotide sequence and second exogenous nucleotide sequence, wherein the first exogenous nucleotide sequence encodes a transaminase and the second exogenous nucleotide sequence encodes a reductase. A method for producing a polyketide compound or a derivative thereof using the aforementioned genetically modified microorganism is also provided.

The present invention provides engineered pyruvate oxidase polypeptides useful for the conversion of pyruvate, phosphate, and oxygen to acetyl phosphate, carbon dioxide, and hydrogen peroxide, as well as compositions and methods of utilizing these engineered polypeptides.

The present application provides phosphoketolase mutants, related products thereof and applications thereof in production of acetyl phosphate, acetyl-CoA and / or 3-hydroxypropionic acid. The phosphoketolase mutants provided by the present application have high catalytic capacity for both six-carbon ketose and five-carbon ketose, and can catalyze the conversion of six-carbon ketose and five-carbon ketose into acetyl phosphate with high efficiency, and then efficiently generate subsequent products acetyl-CoA and 3-hydroxypropionic acid, thereby having great application potential in production of acetyl-CoA and products (such as 3-hydroxypropionic acid) taking acetyl-CoA as a precursor.

The invention discloses recombinant corynebacterium glutamicum for high yield of N-acetylglucosamine, which is characterized in that corynebacterium glutamicum ATCC13032 is used as an original strain; an N-acetylglucosamine-6-phosphate deacetylase encoding gene nagA, a glucosamine-6-phosphate deaminase encoding gene nagB, a glucose-6-phosphate 1-dehydrogenase encoding gene zwf, a lactic dehydrogenase encoding gene ldhA, an N-acetylmannosamine-6-phosphate 2-epimerase encoding gene nano E, a glucosamine-6-phosphate deacetylase encoding gene nagA, a glucosamine-6-phosphate deacetylase encoding gene nagB, a glucose-6-phosphate 1-dehydrogenase encoding gene zwf, a lactic dehydrogenase encoding gene ldhA, a lactic dehydrogenase encoding gene ldhA, a an N-acetylglucosamine specific phosphotransferase encoding gene cgl2642 and a glucose transporter encoding gene ptsG are used as the specific gene of the N-acetylglucosamine specific phosphotransferase; the invention also discloses a method for preparing the recombinant glucosamine-6-phosphate acetyltransferase gene, and expresses an exogenous glucosamine-6-phosphate acetyltransferase coding gene GNA1, an exogenous glutamine-fructose-6-phosphate transaminase coding gene glmS, an exogenous glucose transporter coding gene galP and an exogenous glucose transporter coding gene glf.

The invention relates to a method for production of acetyl phosphate from renewable or next generation feedstocks, comprising enzymatically converting a non-phosphorylated carbohydrate into acetyl phosphate and a product, with a phosphoketolase. In embodiments, the phosphoketolase comprises at least one mutation in its active site preferably enabling an increased affinity and / or increased phosphoketolase activity for a non-phosphorylated carbohydrate, such as i) D-fructose, (ii) D-erythrulose or (iii) glycolaldehyde, compared to a wild- type phosphoketolase sequence. The invention further relates to a phosphoketolase enzyme comprising in its active site the mutation(s) H548N, N549D, H256Y, T2A:16T:H260Y:H548N, H548Y, H260Y:H548Y, H256Y:H260Y:H548Y, H142N:E153D and / or T2A:16T:H260Y:H548Y and its use for producing acetyl phosphate from a non-phosphorylated carbohydrate. The invention further relates to non-human organisms, preferably a microorganism, comprising a gene encoding and optionally expressing said phosphoketolase enzyme and compositions comprising the same.