A kind of preparation method of glutamic acid dipeptide

A technology of dipeptide and alanine, applied in biochemical equipment and methods, peptides, transferases, etc., can solve the problems of high production cost, complex synthesis process, unfavorable industrial scale-up, etc., achieve low cost, wide source of raw materials, easy The effect of large-scale industrial production
CN106834394BActive Publication Date: 2020-11-06TIANJIN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV OF SCI & TECH
Publication Date
2020-11-06

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Abstract

The invention relates to a preparation method of glutamine dipeptide. Escherichia coli is used for expressing amino acid ligase from bacillus subtilis and acetokinase from clostridium acetobutylicum respectively, and a double-enzyme coupling system is formed through mixing after purification. The amino acid ligase is used for catalyzing L-alanine and L-glutamine to generate the glutamine dipeptide, meanwhile ADP is formed along with ATP dephosphorylation, and the acetokinase is used for catalyzing acetyl phosphate and the ADP to form ATP, so that cyclic regeneration of the ATP is realized. By using the double-enzyme coupling system, 32.5 mM of glutamine dipeptide can be obtained through reaction for 8 h under appropriate reaction conditions, and the molar conversion rate is 64.5%. The production method of the glutamine dipeptide provided by the invention has the advantages of low raw material cost, short enzymatic conversion time, simple and convenient operation, low production cost and the like, and has relatively high industrial application value.
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Description

technical field

[0001] The invention relates to the field of biotechnology production of nucleoside products, in particular to a preparation method of glucodipeptide. Background technique

[0002] As an essential amino acid for human metabolism, L-glutamine is converted into glycosamine in the body. As a precursor for the synthesis of mucin, it can promote ulcer healing. It is used as a parenteral nutrition drug for severe infection, compound fractures, and trauma. , major surgery, large area burns, treatment and recovery of patients with chemical poisoning injuries, radiation injuries, and harmful substances; it can also be used as a brain function improving agent for improving brain function in children with mental retardation and mental disorders, alcoholism, and epilepsy. In addition, it can also be used as a therapeutic drug for immunodeficiency syndrome such as AIDS, critical illness or immunocompromised patients after bone marrow transplantation, so it is widely used ...

Claims

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