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Method for reducing proportion of succinic acid fermentation by-products of actinobacillus succinogenes

A technology of actinomycetes and succinic acid, which is applied in the field of fermentation engineering, can solve the problems of high proportion of by-products and reduce the proportion of by-products of succinic acid fermentation, and achieve the effects of low cost, low content and low accumulation concentration

Active Publication Date: 2020-04-07
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for reducing the proportion of by-products of Actinobacillus succinogenes succinic acid fermentation in view of the problem that the proportion of by-products in the production of succinic acid fermentation is too high

Method used

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  • Method for reducing proportion of succinic acid fermentation by-products of actinobacillus succinogenes
  • Method for reducing proportion of succinic acid fermentation by-products of actinobacillus succinogenes
  • Method for reducing proportion of succinic acid fermentation by-products of actinobacillus succinogenes

Examples

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Embodiment 1

[0025] A method for reducing the ratio of succinic acid fermentation by-products of Actinobacillus succinate, comprising the following steps:

[0026] (1) Preparation of culture medium

[0027] The composition and concentration of the seed medium (g / L): glucose 20, yeast extract 10, corn steep liquor 5, NaCl 2, NaHCO 3 2. NaH 2 PO 4 8.5,K 2 HPO 4 15.5, sterilized at 115°C for 20 minutes;

[0028] Composition and concentration of fermentation medium (g / L): glucose 50, yeast extract 10, corn steep liquor 15, NaHCO 3 4, (NH 4 ) 2 SO 4 2. MgCl 2 .6H 2 O1, CaCl 2 0.3, MnCl 2 0.08, ZnSO 4 0.08, basic MgCO 340, sterilized at 115°C for 20 minutes;

[0029] (2) Seed cultivation

[0030] Inoculate the Actinobacillus succinogenes PZ on the plate into the seed medium, and culture it in an anaerobic box at 37°C or in a common incubator for 16 hours, and the number of bacteria reaches more than 300 million to obtain first-class seeds; then the volume ratio is 5% of ...

Embodiment 2

[0037] Control group: fermentation without addition of bisulfite or metasulfite

[0038] (1)~(2) are with embodiment 1 step (1)~(2);

[0039] (3) Insert the secondary seeds into a 250mL anaerobic bottle with 150mL fermentation medium at an inoculum size of 10% by volume. 2 Fermentation in an environment, use basic magnesium carbonate to adjust the fermentation pH to maintain at 6.5-7.0, and carry out fermentation at a speed of 200r / min and a temperature of 37°C for 60h. After the fermentation is over, samples are taken to detect the content of organic acids. The fermentation results are shown in the figure figure 1 shown.

Embodiment 3

[0041] A method for reducing the ratio of succinic acid fermentation by-products of Actinobacillus succinate, comprising the following steps:

[0042] (1) Preparation of culture medium

[0043] The composition and concentration of the seed medium (g / L): glucose 20, yeast extract 10, corn steep liquor 5, NaCl 2, NaHCO 3 2. NaH 2 PO 4 8.5,K 2 HPO 4 15.5, sterilized at 115°C for 20 minutes;

[0044] Composition and concentration of fermentation medium (g / L): glucose 70, yeast extract 10, corn steep liquor 15, NaHCO 3 4, (NH 4 ) 2 SO 4 2. MgCl 2 .6H 2 O1, CaCl 2 0.3, MnCl 2 0.08, ZnSO 4 0.08, basic MgCO 3 Sterilize at 60,115°C for 20 minutes;

[0045] (2) Seed cultivation

[0046] Inoculate the Actinobacillus succinogenes PZ on the plate into the seed medium, and culture it in an anaerobic box at 37°C or in a common incubator for 16 hours, and the number of bacteria reaches more than 300 million to obtain first-class seeds; then the volume ratio is 5% of ...

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Abstract

The invention discloses a method for reducing the proportion of succinic acid fermentation by-products of actinobacillus succinogenes, comprising the following steps: carrying out fermentation cultureon strains subjected to strain activation and propagation, with the inoculum size of 5-15% by volume; and adding 1-20 g / L of hydrosulphite or metabisulfite into the fermentation medium. According tothe invention, hydrosulphite or metabisulfite is added in the process of producing succinic acid by fermentation of actinobacillus succinogenes, and is subjected to addition reaction with an intermediate product acetyl phosphate generated in cells to hinder further conversion into acetic acid, so that the content of acetic acid by-products can be greatly reduced under the condition of not influencing the yield of succinic acid. The process is simple in operation process and low in cost, and has industrial application value.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a method for reducing the ratio of succinic acid fermentation by-products of Actinobacillus succinogenes. Background technique [0002] Succinic acid, also known as succinic acid, is a C4 dibasic carboxylic acid that naturally exists in animal, plant, human and microbial cells. mid product. Succinic acid can be used as a precursor or intermediate product in the synthesis of various chemical substances, and has a wide range of applications in the fields of food, medicine, chemical industry, and spices. For example, succinic acid is a precursor for the synthesis of important compounds such as 1,4-butanediol, γ-butyrolactone, tetrahydrofuran, N-methylpyrrolidone, and adipic acid. In addition, succinic acid is also an important raw material for the synthesis of a biodegradable material, polybutylene succinate (PBS). [0003] Industrial production of succinic acid mainly ado...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N1/20C12N1/38C12R1/01
CPCC12N1/20C12N1/38C12P7/46
Inventor 李亿王青艳秦艳朱婧梁戈彭龙云潘丽霞
Owner GUANGXI ACAD OF SCI
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