69 results about "Actinobacillus succinogenes" patented technology

This invention relates to a method for producing succinic acid type culture by animalcule fermentation, especially relating to an animalcule actinobacillus succinogenes SW0580 generating succinic acid by a fermentated sugariness raw material, its preservation number: CGMCC No. 1593 and a method for utilizing said animalcule fermentation to produce succinic acid, in which, said animalcule is the actinobacillus succinogenes separated from the rumen tested and 10-50g/L succinic acid can be produced by anaerobic fermentating 20-100g/L sugariness raw materials.

The invention discloses a method for producing butane diacid through continuous fermentation or semi-continuous fermentation, which belongs to the bioengineering technical field. The invention provides application of Actinobacillus succinogenes in the method for continuously or semi-continuously preparing the butane diacid by utilizing carbohydrate raw materials such as cane molasses, corn starch syrup, Jerusalem artichoke hydrolysis syrup, sweet sorghum straw syrup, and lignocellulose hydrolysis syrup and so on. The method utilizes multi-step continuous fermentation or two-step semi-continuous fermentation, which can improve germ concentration and cell activity and can obtain high butane diacid output and high butane diacid production intensity; the method is easy to realize automatic and continuous operation; compared with batch fermentation, the method can save non-fermentation time such as repeated tank cleaning, sterilization and so on, so the production efficiency can be greatly improved; the semi-continuous fermentation for producing the butane diacid is easier to control fermentation parameters than the continuous fermentation, has high sugar utilization rate, target product yield and target product output, has simple and easy equipment and operation, and is suitable for industrialized production.

An Actinobacillus succinogenes plasmid vector which provides a means to overexpress proteins in A. succinogenes. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in A. succinogenes. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in Actinobacillus succinogenes, an origin of replication functional in Actinobacillus succinogenes, a second promoter isolated from Actinobacillus succinogenes, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of Actinobacillus succinogenes, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinant Actinobacillus succinogenes is described, including a method of transformation. Additionally, a recombinant Actinobacillus succinogenes is disclosed and a method for producing succinate utilizing this recombinant Actinobacillus succinogenes is described.

The invention discloses a method for producing succinic acid by the simultaneous saccharification and fermentation of a straw raw material and belongs to the technical field of bioengineering. In the method, a straw agricultural waste serving as a raw material is synchronously saccharified and fermented by microorganisms to form the succinic acid; or straws are cut, grounded and pretreated by a diluted alkali to form succinic acid under the coactions of cellulose generated by trichoderma reesei and trichoderma viride and actinobacillus succinogenes; or the straws pretreated by the 70g/L diluted alkali forms 35.5g/L succinic acid through anaerobic fermentation at 38 DEG C. The method has the advantages of cheap and non-grain raw material, low equipment investment and operation cost, environment protection and alleviation of the dependency of chemical synthesis on petrochemical resources.

Actinobacillus succinogenes genes and methods of using the genes in genetically engineered A. succinogenes so as to improve production of chemicals such as succinate, fumarate, malate, 5-aminolevulinate, 2-oxoglutarate, glutamate, and aspartate. The genetically engineered A. succinogenes strains are capable of overexpressing C4 enzymes. The genetically engineered A. succinogenes can have one or more gene knockouts or modifications that inhibit C3 enzymes. The fluxes supplying substrate to the C4 pathway can also be improved in some of the genetically engineered A. succinogenes.

The invention discloses an actinobacillus succinogenes GXAS-137, and a method for producing succinic acid by screening and fermentation of the strain; the strain is obtained by enrichment and screening from a cattle rumen; the method comprises the following steps: inoculating the actinobacillus succinogenes strains in a seed medium, culturing for 24-36 hours in an anaerobic incubator or a common incubator at 30-38 DEG C in a static manner, inoculating into a fermentation medium with an inoculation amount of 1-10%, adjusting the pH to 6.0-7.0, and performing fermentation at 30-38 DEG C for 24-120 hours to obtain succinic acid. Fermentation research is performed with different raw materials and in different media; the succinic acid yield is up to 70 g/L; and the method has potential for industrial production.

The invention discloses actinobacillus succionogenes capable of producing succinic acid with high yield and a method for screening and producing the succinic acid by a fermentation method. The actinobacillus succinogenes takes CGMCC1593 as the original strain and is obtained by blending a plurality of turns of protoplast in a progressive way through a method of '96-pore plate culture, HPLC concentration detection and then anaerobic bottle re-screening'. The actinobacillus succinogenes has already been preserved on February 26, 2012 in China Center for Type Culture Collection with the preservation number CCTCCNO: M2012036. The actinobacillus succinogenes adopts fed-batch culture in a 5 to 15 L fermentation tank, 95.6 g/L succinic acid is produced in 48 hours, the production intensity is 1.99 g/(L.h), and the saccharic acid transformation rate is 0.71 g/g. Compared with other bacterial strains at home and abroad, the actinobacillus succinogenes has the advantages of high yield and lowerproduction cost.

This invention discloses a method for producing succinic acid from waste syrup by fermentation. The method comprises: screening rumen to obtain Actinobacillus succinogenes (CGMCC 1593), and performing fed batch anaerobic fermentation on waste syrup (reducing sugar concentration = 50-100 g/L) in the presence of CO2 or N2 at a pH value of 5.5-7.5 for 30-60 h. The concentration of succinic acid can reach 30-60 g/L. The conversion rate is 70-84%, the productivity is 0.9-1.2 g/L.h, and the sugar utilization rate is 90-95%. The method utilizes renewable resource (waste syrup) instead of pure glucose as the carbon source to produce succinic acid. The method has a low cost, and is environmentally friendly.

The invention discloses a single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid, which comprises the procedures including that: firstly, seed liquid of high-activity actinobacillus capable of producing succinic acid is prepared; secondly, the seeds are proliferated in cultivation with a10L pot; thirdly, the 10L-pot solified cells are prepared; fourthly, the repeated single-pot fermentation with the 10L pot is carried out, then the seeds are inoculated into a fresh substrate, the first batch of fermentation is controlled within 24 hours, followed by 9 batches of continuous fermentation, each of which lasts for 16 hours, then the amphoteric anaerobic cultivation pattern is controlled through the current velocity of the combined air of CO2 and H2, the pH of the fermentation liquid is controlled with fed-batch of calcium oxide, wherein, at the end of each batch of fermentation, the next batch of fermentation is carried out through intercepting the solidified cells with positive-pressure sterilized operation with a propeller filter arranged inside the pot, the fermentation intensity is stabilized above 3.10g/(h.L), and the single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid is completely accomplished.

The invention discloses a bacterial strain capable of enhancing glycerin metabolism. The bacterial strain is classified and names as Actinobacillussuccinogenes JF1315, already collected at the China Center for Type Culture Collection on 31th, Mar. 2016 under CCTCC NO: M 2016160. The invention further relates to application of Actinobacillussuccinogenes JF1315 in producing organic acid through fermentation. Compared with control bacterial strains, under an anaerobic condition, the bacterial strain can grow and metabolizes at low pH, and reductive organic acid can be synthesized efficiently by utilizing glycerin fermentation. When pH is 4.8-6.8, the bacterial strain can normally grow and metabolize glucose to synthesize the organic acid like succinic acid; in addition, under the condition, the bacterial strain can efficiently utilize glycerin for anaerobic fermentation to synthesize and accumulate the organic acid.

The invention discloses a method for producing succinic acid by multistage continuous fermentation. The method comprises the following steps: firstly, preparing a seed culture medium, a primary fermentation culture medium, a carbon source replenishing tank culture liquid and a medium replenishing tank culture liquid; secondly, sterilizing a serial fermentation tank set, a carbon source replenishing tank, a culture medium replenishing tank, the seed culture medium, the primary fermentation culture medium, the carbon source replenishing tank culture liquid, the medium replenishing tank culture liquid and a serum bottle; thirdly, inoculating the sterilized seed culture medium into the sterilized serum bottle, introducing CO2, inoculating actinobacillus succinogenes NJ113, and performing shake cultivation to serve as a fermentation strain; fourthly, performing multistage continuous fermentation. As the production method is simple and the yield and purity of succinic acid are high, the method is suitable for industrialized production.

The invention discloses a method for producing high-performance white carbon black and succinic acid by comprehensively utilizing white spirit lees, which comprises the following steps of: taking lees after fermenting white spirit as a raw material, and separating lees residues and rice husks after carrying out water washing; taking the lees residues for carrying out filter pressing, then carrying out airflow drying, pulverizing, sieving by a sieve with 80 to 100 meshes, fermenting by using actinobacillus succinogenes, carrying out filter pressing again, afterwards discoloring by active carbon and extracting so as to obtain succinic acid; and taking the rice husks after separating, burning, pulverizing the ashes of the rice husks, sieving by a sieve with 80 to 100 meshes, carrying out acid washing, filtering, carrying out alkali dissolving, leaching, adding a chelating agent for curing, aging, washing and carrying out spray drying so as to obtain high-performance white carbon black. According to the method for producing the high-performance white carbon black and the succinic acid by comprehensively utilizing the white spirit lees, industrial solid waste white spirit lees are taken as the raw material, high and new technologies, such as a biological fermenting technology and the like, and an advanced process for the full utilization and the zero discharge of products generatedin procedures are adopted in an integrated way, and products, such as the high-performance white carbon black, the succinic acid and the like, are developed. The method for producing the high-performance white carbon black and the succinic acid by comprehensively utilizing the white spirit lees has higher popularization value.

The invention belongs to the technical field of soil remediation, and particularly relates to a complex microbial agent for efficiently converting heavy metal chromium in soil as well as a preparation method and application thereof. The complex microbial agent is obtained by mixing and carrying out enrichment culture on the following two florae: a flora A consisting of bacillus, pediococcus acidilactici, clostridium coriolis, actinobacillus succinogenes and bacillus aceticus; a flora B consisting of acidophilic iron bacteria, thiobacillus ferrooxidans, acid-thiobacillus thiooxidans, sulfur-oxidizing acid-thiobacillus thiooxidans, sulfur-oxidizing bacteria and acidophilic thiobacillus ferrooxidans. After the complex microbial agent is used for treating the chromium-contaminated soil, the chromium removal rate can reach 91% or above. The complex microbial agent is formed by culturing and mixing a plurality of strains, and the strains have a good synergistic effect and do not generate antagonism; after compounding, the adaptability to heavy metals can be improved, so that the survival and removal effects of the soil in heavy metal contaminated soil are improved. The microbial agent is simple in preparation and application method, short in culture period and treatment period and low in cost, prevents secondary pollution, and has a wide application prospect.

The invention provides an actinobacillus succinogenes PZ improving yield of succinic acid, and a construction method thereof. The construction method comprises the steps of constructing an expression carrier, constructing recombinant plasmid and constructing the actinobacillus succinogenes PZ. The actinobacillus succinogenes starting strain is connected in series with and expressed excessively by gene pepck of a key rate-limiting enzyme, PEPCK, for generating succinic acid by using the actinobacillus succinogenes, and gene zwf of G6PDH improving the restoring force level in a body, so as to acquire the actinobacillus succinogenes PZ. The actinobacillus succinogenes PZ can improve the yield of succinic acid, and has the potential of industrial production application.

The invention provides a method for producing succinic acid by utilizing fermentation of actinobacillus succinogenes. The method includes taking canna edulis ker syrup as a main material, producing the succinic acid by using the fermentation of actinobacillus succinogenes CICC11014, determining the optical fermentation medium for bacterial strains through research for medium of bacterial strains; and fermenting the medium after optimization, wherein the medium comprises 89.66g/L of canna edulis ker syrup (by total suger), 38.55g/L of CSL, 2.23g/L of Na2HPO4*12(H2O), 2.23g/L of KH2PO4*3(H2O),60g/L of MgCO3, and 6.5 of the pH; and performing moist heat sterilization at a temperature of 115 DEG C for 20 minutes. The conversion rate of sugar can be improved through fermentation under the optical medium condition, so that the yield of the succinic acid can reach 70g/L.

The invention discloses a strain and a method for producing succinic acid by fermenting xylose mother liquor. The strain is actinobacillus succinogenes PZ, and has a preservation number of CCTCC (China Center For Type Culture Collection) No: M2016396. The actinobacillus succinogenes can be used for producing the succinic acid by fermenting the high-concentration xylose mother liquor; the maximum yield of the succinic acid can reach 95g/L, and the maximum production rate of the succinic acid is 85%. The method provided by the invention is low in cost, high in yield and simple in technology, realizes the efficient resource utilization of the xylose mother liquor, and has great significance on resource optimization and environmental protection.

The invention discloses a method for producing succinic acid by means of fermentation. The method includes steps of constructing acid-producing bacterial strains; producing the succinic acid from the recombinant bacterial strains by means of fermentation. Original bacterial strains for constructing the recombinant bacterial strains are actinobacillus succinogenes NJ113 (with a preservation number of CGMCC No.1716). The method for producing the succinic acid has the advantages that processes for acquiring the acid-producing bacterial strains are simple and convenient, fermentation processes are simple and feasible, the method is high in acid-producing capacity, the production cost can be greatly reduced, and economic benefits can be increased; the recombinant bacterial strains can grow in synthetic culture media without culture media with added exogenous glutamic acid and are high in succinic acid yield; the 19.53 g/L succinic acid can be produced after 27 g/L glucose which is used as a carbon source is fermented in an anaerobic serum bottle for 72 h, and the succinic acid yield can reach 72.33%.