Method for producing amber acid by continuous fermentation or semi-continuous fermentation

A semi-continuous, succinic acid technology, applied in the field of bioengineering, to achieve the effects of high production intensity, saving energy and water consumption, and saving labor

Inactive Publication Date: 2008-11-12
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] So far, there is no example of continuous fermentation ...

Method used

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  • Method for producing amber acid by continuous fermentation or semi-continuous fermentation
  • Method for producing amber acid by continuous fermentation or semi-continuous fermentation
  • Method for producing amber acid by continuous fermentation or semi-continuous fermentation

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1, single-stage continuous fermentation

[0055] 1) Carry out single-stage continuous fermentation in a reactor with a liquid content of 200mL, the total sugar concentration of molasses in the fed-batch medium is 30g / L, adjust the medium flow velocity (12mL / h), and carry out continuous culture, the fermentation time curve See Figure 4 . Depend on Figure 4 It can be seen that the continuous fermentation lasted for 10 days, and the average dilution rate was 0.053h -1 , the concentration of succinic acid and the concentration of bacteria remained stable at 19.2g / L respectively, and the OD of the fermentation broth was diluted 10 times 660 0.51 or so, the production intensity of succinic acid is 1.15g / (L·h).

[0056] 2) According to the fermentation conditions of 1), the total sugar concentration of molasses in the fed-batch medium is 35g / L, respectively at a dilution rate of 0.06-0.91h -1 Continuous fermentation was carried out within the range, and the r...

Embodiment 2

[0060] Embodiment 2, multistage continuous fermentation

[0061] A multi-stage single-flow continuous fermentation device is adopted, the effective liquid volume of a single tank is 200mL, the total sugar concentration of molasses in the feeding medium is 35g / L, and the total dilution rate is controlled at 0.11h -1 , 0.21h -1 and 0.30h -1 , the results are shown in Table 2.

[0062] Table 2 Production of succinic acid by multi-stage single-flow continuous fermentation

[0063]

[0064] From Table 2, it can be found that the succinic acid output of increasing the number of stages of the reactor is higher than that of the single-stage continuous fermentation, and the dilution rate of the two-stage continuous fermentation is 0.11h -1 The maximum concentration of succinic acid reaches 19.0g / L, which is 0.11h higher than that of single-stage continuous fermentation -1 increased by 42.3%; the dilution rate is 0.30h -1 , the production intensity reaches up to 4.29g / (L h), whi...

Embodiment 3

[0066] Embodiment 3, single stage semi-continuous fermentation produces succinic acid

[0067] Fermentation was carried out in a 250 mL reactor with a feed bottle volume of 2 L. With the initial total sugar concentration of 40g / L and 60g / L respectively, each batch of fermentation time is 8-24h, extract the fermentation broth volume (V) / reactor liquid volume (V 0 )=50%, and the growth and acid production of the bacteria are shown in Table 3.

[0068] Table 3 Production of succinic acid by single-stage semi-continuous fermentation of sugarcane molasses

[0069]

[0070] As can be seen from Table 3, the semi-continuous fermentation time is 10-12h, and the yield and production intensity of succinic acid are relatively high, which can reach 33.67g / L and 1.63g / (L h) respectively. Affect the next batch of acid production speed.

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Abstract

The invention discloses a method for producing butane diacid through continuous fermentation or semi-continuous fermentation, which belongs to the bioengineering technical field. The invention provides application of Actinobacillus succinogenes in the method for continuously or semi-continuously preparing the butane diacid by utilizing carbohydrate raw materials such as cane molasses, corn starch syrup, Jerusalem artichoke hydrolysis syrup, sweet sorghum straw syrup, and lignocellulose hydrolysis syrup and so on. The method utilizes multi-step continuous fermentation or two-step semi-continuous fermentation, which can improve germ concentration and cell activity and can obtain high butane diacid output and high butane diacid production intensity; the method is easy to realize automatic and continuous operation; compared with batch fermentation, the method can save non-fermentation time such as repeated tank cleaning, sterilization and so on, so the production efficiency can be greatly improved; the semi-continuous fermentation for producing the butane diacid is easier to control fermentation parameters than the continuous fermentation, has high sugar utilization rate, target product yield and target product output, has simple and easy equipment and operation, and is suitable for industrialized production.

Description

technical field [0001] A method for producing succinic acid by continuous fermentation or semi-continuous fermentation, which belongs to the technical field of bioengineering. Specifically, the present invention relates to continuous fermentation or semi-continuous fermentation method in succinic acid Actinobacillus (Actinobacillus succinogenes) CGMCC1593 fermentation sugar cane molasses, cornstarch syrup, Jerusalem artichoke hydrolysis syrup, sweet sorghum straw syrup and cellulosic wood raw material hydrolysis syrup and other carbohydrates The application of compound raw materials in the process of preparing succinic acid. Background technique [0002] Succinic acid, also known as succinic acid, is an important four-carbon compound in industry. It is widely used as organic synthesis raw materials, intermediate products or professional chemicals in food, medicine, pesticides, dyes, spices, paints , plastics and materials industry. Its largest potential market is in areas ...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12N1/20C12R1/01
Inventor 孙志浩过鑫富郑璞倪晔董晋军
Owner JIANGNAN UNIV
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