Marsh gas engineering inoculum and preparation method thereof

A technology of engineering bacteria and biogas, applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as not being able to meet large-scale energy utilization, project operation efficiency needs to be improved, and negative impact on the reputation of biogas projects , to achieve the effects of shortening hydrolysis and acidification time, improving gas production efficiency and gas production stability, and shortening fermentation start-up time

Active Publication Date: 2015-07-29
马力通
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Restricted by economic conditions, many biogas projects adopt normal temperature fermentation process, and do not take any heat preservation or temperature increase measures in winter, resulting in low gas production rate, especially in winter, when the gas production rate drops sharply, and winter is exactly When the gas consumption is large, it has a negative impact on the reputation of the biogas project, and the operation efficiency of the project needs to be improved
[0007] (2) The source of raw materials is single, most of which are livestock and poultry manure and crop straw. The anaerobic fermentation speed is slow and the fermentation cycle is long, which cannot meet the needs of large-scale energy utilization such as biogas power generation or vehicle bio-natural gas.
[0025] In addition, domestic biogas projects mostly purchase commercial functional microbial agents from abroad in order to ensure efficient gas production. The price is high and there is a technical blockade. Domestic biogas projects are in a stage of rapid development, and the purchase volume cannot meet the domestic demand that grows year by year.
Moreover, the medium and long-term impact of foreign products on the environment of our country, and the impact of sewage reuse of residual bacterial agents on soil, crops, and groundwater have not been demonstrated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Take 50kg of biogas engineering biogas slurry with a total solid Ts of 1.8%, and culture it statically at 28°C. First add 1.2% mass fraction of 60-mesh Baiyun Obo iron ore powder, and then add 0.6 mass fraction every 2 days % that is, 0.3kg 60 mesh pig manure until the pH of the culture solution drops to 6.4, and then add a mass fraction of 2.0% every 2 days, that is, 1kg 60 mesh pig manure, until the coenzyme F420 fluorescence spectrophotometry value of the culture solution is 58.2, and then the obtained culture Add 40 meshes of pig manure adjusted to a pH of 7.0 to a moisture content of 50%, the mass ratio of pig manure to culture solution is 1.5:1, and place it at 30°C to dry for 96 hours to obtain a biogas engineering bacterial agent. The bacterial agent was detected by PCR-DGGE, mainly by Dyella marensis , Roseospira goensis , Clostridium lactatifermentans , Clostridium beijerinckii , Kosmotoga olearia , Aminobacterium colombiense , Bacteroi...

Embodiment 2

[0042] Example 2: Take 100kg of urban sewage treatment plant sludge with a total solid Ts of 7%, shake it at 35°C at 60 rpm, add 2.5% mass fraction of 100-mesh Baiyun Obo iron ore powder, and then add mass fraction every other day Fraction 1%, that is, 1kg 60 mesh cow dung until the pH of the culture medium drops to 6.5, and then add a mass fraction of 2% every 3 days, that is 2kg 60 mesh, according to the 1:1 mixture of cow dung and corn stalks until the culture medium coenzyme F420 fluorescence The spectrophotometric value is up to 89.6, and then the obtained culture solution is added with a mixture of 40 meshes of cow dung and peat with a pH of 7.0 and half of the weight until the water content is 60%, and the mass ratio of the mixture to the culture solution is 2.5:1. Dry at 40°C for 60 hours to obtain biogas engineering bacteria agent. The bacterial agent was detected by PCR-DGGE, mainly by Clostridium beijerinckii , Aminobacterium colombiense, Clostridium cellulovora...

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PUM

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Abstract

The invention discloses a marsh gas engineering inoculum and a preparation method thereof, and aims to solve the problems of low anaerobic fermentation speed, long fermentation period and poor marsh gas engineering gas productivity and engineering running efficiency in the conventional marsh gas engineering. Organic waste sludge or biogas slurry is taken as a raw material, and a special marsh gas engineering inoculum is prepared through mixed culture and a fixed anaerobic fermentation microbial flora according to an anaerobic fermentation principle and a microbial ecology principle. Compared with the prior art in which an inoculum is prepared by culturing single strains respectively and mixing proportionally, the preparation method has the advantages that the inoculum is prepared through mixed culture of sludge or biogas slurry is stable in micro-ecology; moreover, the marsh gas engineering fermentation period can be remarkably shortened, the marsh gas yield is increased, and the marsh gas engineering economical benefit is increased.

Description

technical field [0001] The invention belongs to the application field of clean energy such as biogas and methane, and in particular relates to a preparation method of biogas engineering bacterial agent. Background technique [0002] Biogas (biological natural gas) is a combustible mixed gas mainly composed of methane produced by microbial fermentation. As an important part of clean energy, biogas has the advantages of both fossil energy (especially natural gas) and renewable energy, and has been commercialized and industrialized in the United States, Britain, Germany, France, Sweden, Switzerland and other countries. Supplements and partial substitutions. [0003] At present, the construction scale and usage of rural household biogas digesters in China rank first in the world, and it has become one of the renewable energy development fields in China with the most mature technology, the largest promotion scale and the most profitable. Various types of energy ecological model...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/20C12R1/01C12R1/13
CPCC12M21/04Y02E50/30
Inventor 马力通李珺
Owner 马力通
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