Marsh gas engineering inoculum and preparation method thereof

Abstract

The invention discloses a marsh gas engineering inoculum and a preparation method thereof, and aims to solve the problems of low anaerobic fermentation speed, long fermentation period and poor marsh gas engineering gas productivity and engineering running efficiency in the conventional marsh gas engineering. Organic waste sludge or biogas slurry is taken as a raw material, and a special marsh gas engineering inoculum is prepared through mixed culture and a fixed anaerobic fermentation microbial flora according to an anaerobic fermentation principle and a microbial ecology principle. Compared with the prior art in which an inoculum is prepared by culturing single strains respectively and mixing proportionally, the preparation method has the advantages that the inoculum is prepared through mixed culture of sludge or biogas slurry is stable in micro-ecology; moreover, the marsh gas engineering fermentation period can be remarkably shortened, the marsh gas yield is increased, and the marsh gas engineering economical benefit is increased.

Description

technical field

[0001] The invention belongs to the application field of clean energy such as biogas and methane, and in particular relates to a preparation method of biogas engineering bacterial agent. Background technique

[0002] Biogas (biological natural gas) is a combustible mixed gas mainly composed of methane produced by microbial fermentation. As an important part of clean energy, biogas has the advantages of both fossil energy (especially natural gas) and renewable energy, and has been commercialized and industrialized in the United States, Britain, Germany, France, Sweden, Switzerland and other countries. Supplements and partial substitutions.

[0003] At present, the construction scale and usage of rural household biogas digesters in China rank first in the world, and it has become one of the renewable energy development fields in China with the most mature technology, the largest promotion scale and the most profitable. Various types of energy ecological model...

Examples

Embodiment 1

[0041] Example 1: Take 50kg of biogas engineering biogas slurry with a total solid Ts of 1.8%, and culture it statically at 28°C. First add 1.2% mass fraction of 60-mesh Baiyun Obo iron ore powder, and then add 0.6 mass fraction every 2 days % that is, 0.3kg 60 mesh pig manure until the pH of the culture solution drops to 6.4, and then add a mass fraction of 2.0% every 2 days, that is, 1kg 60 mesh pig manure, until the coenzyme F420 fluorescence spectrophotometry value of the culture solution is 58.2, and then the obtained culture Add 40 meshes of pig manure adjusted to a pH of 7.0 to a moisture content of 50%, the mass ratio of pig manure to culture solution is 1.5:1, and place it at 30°C to dry for 96 hours to obtain a biogas engineering bacterial agent. The bacterial agent was detected by PCR-DGGE, mainly by Dyella marensis , Roseospira goensis , Clostridium lactatifermentans , Clostridium beijerinckii , Kosmotoga olearia , Aminobacterium colombiense , Bacteroi...

Embodiment 2

[0042] Example 2: Take 100kg of urban sewage treatment plant sludge with a total solid Ts of 7%, shake it at 35°C at 60 rpm, add 2.5% mass fraction of 100-mesh Baiyun Obo iron ore powder, and then add mass fraction every other day Fraction 1%, that is, 1kg 60 mesh cow dung until the pH of the culture medium drops to 6.5, and then add a mass fraction of 2% every 3 days, that is 2kg 60 mesh, according to the 1:1 mixture of cow dung and corn stalks until the culture medium coenzyme F420 fluorescence The spectrophotometric value is up to 89.6, and then the obtained culture solution is added with a mixture of 40 meshes of cow dung and peat with a pH of 7.0 and half of the weight until the water content is 60%, and the mass ratio of the mixture to the culture solution is 2.5:1. Dry at 40°C for 60 hours to obtain biogas engineering bacteria agent. The bacterial agent was detected by PCR-DGGE, mainly by Clostridium beijerinckii , Aminobacterium colombiense, Clostridium cellulovora...

technical field

[0001] The invention belongs to the application field of clean energy such as biogas and methane, and in particular relates to a preparation method of biogas engineering bacterial agent. Background technique

[0002] Biogas (biological natural gas) is a combustible mixed gas mainly composed of methane produced by microbial fermentation. As an important part of clean energy, biogas has the advantages of both fossil energy (especially natural gas) and renewable energy, and has been commercialized and industrialized in the United States, Britain, Germany, France, Sweden, Switzerland and other countries. Supplements and partial substitutions.

[0003] At present, the construction scale and usage of rural household biogas digesters in China rank first in the world, and it has become one of the renewable energy development fields in China with the most mature technology, the largest promotion scale and the most profitable. Various types of energy ecological model...

Examples

Embodiment 1

[0041] Example 1: Take 50kg of biogas engineering biogas slurry with a total solid Ts of 1.8%, and culture it statically at 28°C. First add 1.2% mass fraction of 60-mesh Baiyun Obo iron ore powder, and then add 0.6 mass fraction every 2 days % that is, 0.3kg 60 mesh pig manure until the pH of the culture solution drops to 6.4, and then add a mass fraction of 2.0% every 2 days, that is, 1kg 60 mesh pig manure, until the coenzyme F420 fluorescence spectrophotometry value of the culture solution is 58.2, and then the obtained culture Add 40 meshes of pig manure adjusted to a pH of 7.0 to a moisture content of 50%, the mass ratio of pig manure to culture solution is 1.5:1, and place it at 30°C to dry for 96 hours to obtain a biogas engineering bacterial agent. The bacterial agent was detected by PCR-DGGE, mainly by Dyella marensis , Roseospira goensis , Clostridium lactatifermentans , Clostridium beijerinckii , Kosmotoga olearia , Aminobacterium colombiense , Bacteroi...

Embodiment 2

[0042] Example 2: Take 100kg of urban sewage treatment plant sludge with a total solid Ts of 7%, shake it at 35°C at 60 rpm, add 2.5% mass fraction of 100-mesh Baiyun Obo iron ore powder, and then add mass fraction every other day Fraction 1%, that is, 1kg 60 mesh cow dung until the pH of the culture medium drops to 6.5, and then add a mass fraction of 2% every 3 days, that is 2kg 60 mesh, according to the 1:1 mixture of cow dung and corn stalks until the culture medium coenzyme F420 fluorescence The spectrophotometric value is up to 89.6, and then the obtained culture solution is added with a mixture of 40 meshes of cow dung and peat with a pH of 7.0 and half of the weight until the water content is 60%, and the mass ratio of the mixture to the culture solution is 2.5:1. Dry at 40°C for 60 hours to obtain biogas engineering bacteria agent. The bacterial agent was detected by PCR-DGGE, mainly by Clostridium beijerinckii , Aminobacterium colombiense, Clostridium cellulovora...