5 results about "Lignocellulose hydrolysis" patented technology

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

This invention discloses a method for promoting the hydrolysis of lignocellulose into fermentable sugars, belonging to the field of biorefining technology. The method includes the following steps: (1) adding ammonium sulfate to the lignocellulose raw material, then mixing it with a glycerol-water mixture, and pretreating it at a certain temperature and time to destroy the dense structure of the lignocellulose; (2) adding ammonium sulfate and cellulase to the pretreated system, and carrying out a saccharification reaction under suitable conditions to convert it into fermentable sugars. This invention solves the problems of high energy consumption, high pollution, and low saccharification and enzymatic hydrolysis efficiency of traditional pretreatment by leveraging the solubilizing, crystallinity-reducing, and biocatalyst-protecting effects of glycerol and the synergistic effect of ammonium sulfate. Compared with traditional hot water pretreatment, the yield of fermentable sugars is significantly increased, and the pretreatment process does not require strong acids or bases; glycerol can be recycled and reused, significantly reducing production costs and possessing significant industrial application value.

The application discloses a method for synchronously and efficiently metabolizing mixed sugar based on a Pichia pastoris mixed flora and application thereof. The Pichia pastoris mixed flora comprises at least one Pichia pastoris engineering strain for xylose metabolism and one Pichia pastoris engineering strain for oligosaccharide metabolism. By means of functional division of the strains, the interference of glucose signal on the xylose metabolic pathway is physically and genetically isolated, and the synchronous metabolism of xylose and cellulosic oligosaccharide is realized. The metabolic burden of each strain is reduced, the exclusive pathway of each strain can be optimized, the pathway competition and the imbalance of auxiliary factors are avoided, the overall fermentation rate and the sugar conversion rate are improved. The mixed flora system has a certain buffering capacity to environmental fluctuations and is more stable than a single strain. The strategy is particularly suitable for complex lignocellulose hydrolysate, and can effectively solve the problem of asynchronous utilization of multiple fermentable sugars in the lignocellulose hydrolysate and improve the total utilization rate of raw materials. In the process of simultaneous saccharification and fermentation of the mixed flora, the utilization rate of xylose and cellulosic oligosaccharide is improved, and the integrated biorefinery cycle is shortened.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.