13 results about "Lignocellulose hydrolysis" patented technology

The invention discloses a lignocellulose continuous multi-stage steam explosion pretreatment process and device, and relates to the technical field of lignocellulose raw material pretreatment. Comprising the following steps: continuously conveying lignocellulose loaded with a chemical additive into a steam explosion pretreatment reaction tube, carrying out continuous three-stage low-temperature reaction coupling high-pressure explosion treatment, and spraying to obtain a pretreated lignocellulose material; wherein the specific parameters of the continuous three-stage low-temperature reaction coupling high-pressure blasting treatment are as follows: the treatment is performed for 5-9 minutes at the temperature of 80-120 DEG C in the first-stage treatment, the treatment is performed for 2-6 minutes at the temperature of 140-160 DEG C in the second-stage treatment, and the treatment is performed for 1-3 minutes at the temperature of 180-220 DEG C in the third-stage treatment; the pressure is 0.5 to 2.0 MPa. According to the continuous steam explosion lignocellulose complete equipment and the pretreatment method, effective continuous production of biomass raw materials can be achieved, low-inhibitor lignocellulose hydrolysis sugar liquid is obtained, fermentation conversion of downstream microorganisms is facilitated, wastewater discharge and use of chemical auxiliaries are reduced, and commercialized large-scale production is expected to be further achieved.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

This invention discloses a strain that degrades various lignin-derived aromatic compounds and its application in detoxification of hydrolysate. The strain is classified as *Rhodococcus ethereans*. Rhodococcus ether-eating Strain N1, deposited at the China Center for Type Culture Collection (CCTCC) on August 11, 2022, with accession number CCTCC NO: M 20221270, can degrade p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillin, coniferyl alcohol, and syringaldehyde. After lignin depolymerization, derivative phenolic inhibitors are formed, mainly divided into three types: S-type (syringaldehyde), G-type (ferulic acid), and H-type (p-coumaric acid), which affect the fermentation of the strain. The Rhodococcus bacterium in this invention can efficiently degrade SGH-type monomers, achieving a removal efficiency of up to 68.4% when applied to the biological detoxification of hydrolysate inhibitors. Strain N1 has significant application value in improving the conversion efficiency of lignocellulose hydrolysate and reducing the detoxification cost in industrial production.

The application discloses a furaldehyde-tolerant Rhodotorula mucilaginosa strain and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is July 1, 2024, the preservation number is CGMCC NO.31122, and the Rhodotorula mucilaginosa strain is named Rhodotorula mucilaginosa F3.The furaldehyde concentration that can be tolerated by the Rhodotorula mucilaginosa F3 strain is 40mM-45mM, so that the production cost of carotenoids can be reduced, the utilization efficiency of lignocellulose hydrolysate is improved, waste utilization is realized, and good commercial and scientific research values are obtained.

This invention discloses a method for promoting the hydrolysis of lignocellulose into fermentable sugars, belonging to the field of biorefining technology. The method includes the following steps: (1) adding ammonium sulfate to the lignocellulose raw material, then mixing it with a glycerol-water mixture, and pretreating it at a certain temperature and time to destroy the dense structure of the lignocellulose; (2) adding ammonium sulfate and cellulase to the pretreated system, and carrying out a saccharification reaction under suitable conditions to convert it into fermentable sugars. This invention solves the problems of high energy consumption, high pollution, and low saccharification and enzymatic hydrolysis efficiency of traditional pretreatment by leveraging the solubilizing, crystallinity-reducing, and biocatalyst-protecting effects of glycerol and the synergistic effect of ammonium sulfate. Compared with traditional hot water pretreatment, the yield of fermentable sugars is significantly increased, and the pretreatment process does not require strong acids or bases; glycerol can be recycled and reused, significantly reducing production costs and possessing significant industrial application value.

The invention relates to the technical field of microorganisms, and particularly discloses saccharomyces cerevisiae IMPHA-6 and application thereof in fermentation production of ethanol. The invention provides a Saccharomyces cerevisiae strain capable of resisting multiple stress, which is Saccharomyces cerevisiae IMPHA-6, and the preservation number of the Saccharomyces cerevisiae strain is CCTCC (China Center for Type Culture Collection) NO: M 20251412. The saccharomyces cerevisiae strain can solve the problem of low fermentation efficiency caused by a composite inhibitor (such as potassium chloride, ethanol, furfural and vanillin) in the lignocellulose hydrolysate, and can remarkably improve the reducing sugar utilization rate and ethanol yield during fermentation of the corn straw cellulose hydrolysate; a novel efficient microbial resource and method are provided for biomass energy production, and promotion of industrial production of cellulosic ethanol is facilitated.

The invention discloses a method for producing gamma-aminobutyric acid through double-bacterium synergistic fermentation of lignocellulose hydrolysate, and belongs to the technical field of microbial engineering. The method comprises the following steps: constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici FR-XY) engineering bacterium, and constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici) FR-XY; the method comprises the following steps: carrying out staged fermentation culture on lactobacillus hilgardii FR-1012 and recombinant pediococcus acidilactici FR-XY by utilizing lignocellulose hydrolysate, so as to obtain mixed fermentation liquor; centrifuging the mixed fermentation liquor, collecting mixed thalli, resuspending the mixed thalli in a conversion solution containing L-glutamic acid and coenzyme 5-phosphopyridoxal, and carrying out whole-cell conversion reaction to obtain a conversion solution containing gamma-aminobutyric acid. According to the method, the lignocellulose hydrolysate can be utilized for double-bacterium synergistic fermentation, and full utilization of a multi-element carbon source in the lignocellulose hydrolysate and efficient synthesis of gamma-aminobutyric acid are achieved.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to a yarrowia lipolytica engineering bacterium, a construction method thereof and application of the yarrowia lipolytica engineering bacterium in production of succinic acid. Specifically, by establishing a multi-copy expression method of xylose metabolic pathway genes XR, XDH and XK in yarrowia lipolytica, multi-copy integration and high-level expression of the genes are realized, and the constructed yarrowia lipolytica engineering bacteria can be used for preparing the yarrowia lipolytica engineering bacteria under the culture condition of taking xylose and lignocellulose hydrolysate as substrates, so that the yield of the yarrowia lipolytica engineering bacteria is increased, and the yield of the yarrowia lipolytica engineering bacteria is increased. Therefore, the method has a good practical application value.

The invention discloses a beta-xylosidase mutant derived from aspergillus brazilis, a gene, a recombinant plasmid, a modified cell and application, and relates to the technical field of biological genetic engineering. The beta-xylosidase mutant is subjected to amino acid mutation on the basis of a sequence as shown in SEQ ID NO: 1, and the amino acid mutation is single-point mutation or combined mutation of S175F, V293I, Q390E, A449S, N526G, Y565K and N576A. The beta-xylosidase is subjected to specific site combination mutation, so that the enzymatic activity and the thermal stability are synergistically improved, and the xylose yield is remarkably improved when the beta-xylosidase is compounded with xylanase for lignocellulose hydrolysis.

The application discloses a method for synchronously and efficiently metabolizing mixed sugar based on a Pichia pastoris mixed flora and application thereof. The Pichia pastoris mixed flora comprises at least one Pichia pastoris engineering strain for xylose metabolism and one Pichia pastoris engineering strain for oligosaccharide metabolism. By means of functional division of the strains, the interference of glucose signal on the xylose metabolic pathway is physically and genetically isolated, and the synchronous metabolism of xylose and cellulosic oligosaccharide is realized. The metabolic burden of each strain is reduced, the exclusive pathway of each strain can be optimized, the pathway competition and the imbalance of auxiliary factors are avoided, the overall fermentation rate and the sugar conversion rate are improved. The mixed flora system has a certain buffering capacity to environmental fluctuations and is more stable than a single strain. The strategy is particularly suitable for complex lignocellulose hydrolysate, and can effectively solve the problem of asynchronous utilization of multiple fermentable sugars in the lignocellulose hydrolysate and improve the total utilization rate of raw materials. In the process of simultaneous saccharification and fermentation of the mixed flora, the utilization rate of xylose and cellulosic oligosaccharide is improved, and the integrated biorefinery cycle is shortened.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

The application discloses a method for preparing functional humus by using nano-iron composite catalyst to induce directional humification of straw and application thereof, and belongs to the technical field of agricultural solid waste resource utilization and humus synthesis. The method uses agricultural straw as raw material, improves the reaction activity through alkali pre-dissociation, and then uses ternary nano-iron composite catalyst composed of nano-Fe2O3, zero-valent nano-iron and nano-FeCl3 to perform directional hydrothermal humification, so as to induce directional condensation of straw lignocellulose hydrolysis products and enrich acid-containing oxygen and nitrogen functional groups. After the reaction, solid-liquid separation is performed and differential modification is performed, the solid phase is used to prepare nano-iron-based hydrothermal humic carbon for saline-alkali farmland improvement, and the liquid phase is used to prepare functional humic acid foliar fertilizer for crop growth promotion. The application shortens the reaction period to 4 hours, realizes directional regulation of functional groups and high-value utilization of all components of straw, and realizes synchronous improvement of soil fertility and yield increase of soybeans and corn through a matched regional application process.