22 results about "Lignocellulose hydrolysis" patented technology

A method for lignocellulose hydrolysis is provided. The method includes: crushing an agroforestry biomass feedstock, extracting with a toluene-ethanol solution, and drying to obtain a de-extracted feedstock; weighing a metal salt and glycerol for mixing to obtain a type IV deep eutectic solvent; weighing the de-extracted feedstock and adding into the type IV deep eutectic solvent, and reacting for 2 h-4 h at a temperature of 80° C.-120° C. and a stirring speed of 300 rpm-500 rpm to obtain a pretreatment mixture; performing a solid-liquid separation to the pretreatment mixture to obtain a filter residue and a filtrate, and then drying the filter residue after washing to obtain a lignocellulose residue after pretreatment; and performing enzymatic hydrolysis reaction to the lignocellulose residue to obtain fermentable sugar.

The invention provides a three-dimensional microbial flora biological hybrid system capable of efficiently generating electricity by utilizing lignocellulose hydrolysate, and belongs to the technical field of biological batteries. According to the invention, an artificial microbial flora SLB composed of S.oneidensis, Lactococcus lactis and Bacillus subtilis is preliminarily constructed, the bacteria in the artificial microbial flora SLB are respectively subjected to genetic engineering modification, then a carbon cloth anode is replaced by a carbon felt anode, a dispersion liquid GO is added, the electricity generation and conductivity of an anode electroactive biological membrane are further optimized, and the electrochemical performance of the anode electroactive biological membrane is further improved. And finally, a three-dimensional microbial flora biological hybrid system S7L1B1 (at) CFamp capable of directly utilizing the lignocellulose hydrolysate to generate power is constructed. And GO. According to the present invention, the MFC technology is introduced, such that the MFC technology has good lignocellulose hydrolysate degradation and biological power generation capacity, such that the new approach is developed for the high-value utilization of the lignocellulose resource, the solid theoretical basis and the practical guidance are provided, and the application development of the MFC technology in the lignocellulose resource conversion field is promoted.

The invention discloses a construction method of engineering bacteria for producing glycerol and lipid by efficiently fermenting lignocellulose hydrolysate, and belongs to the technical field of microorganisms. The invention provides a method for producing glycerol and lipid by fermenting lignocellulose hydrolysate by using Candida glycinogenes which is resistant to high osmotic pressure as a production strain. According to the present invention, with the Candida glycyinogenes engineering strain Cg-AAGD constructed by using the method, the virus-free lignocellulose hydrolysate (containing about 175 g / L of glucose and 25 g / L of xylose) can be fermented to produce 82.2 g / L of glycerol and 7.3 g / L of lipid; according to the method, the lignocellulose hydrolysate is selected as a raw material, the purpose of deep resource utilization of agricultural waste is achieved, the related technological operation flow is relatively simple and convenient, and the requirement for production equipment is easy to achieve.

The invention discloses a lignocellulose continuous multi-stage steam explosion pretreatment process and device, and relates to the technical field of lignocellulose raw material pretreatment. Comprising the following steps: continuously conveying lignocellulose loaded with a chemical additive into a steam explosion pretreatment reaction tube, carrying out continuous three-stage low-temperature reaction coupling high-pressure explosion treatment, and spraying to obtain a pretreated lignocellulose material; wherein the specific parameters of the continuous three-stage low-temperature reaction coupling high-pressure blasting treatment are as follows: the treatment is performed for 5-9 minutes at the temperature of 80-120 DEG C in the first-stage treatment, the treatment is performed for 2-6 minutes at the temperature of 140-160 DEG C in the second-stage treatment, and the treatment is performed for 1-3 minutes at the temperature of 180-220 DEG C in the third-stage treatment; the pressure is 0.5 to 2.0 MPa. According to the continuous steam explosion lignocellulose complete equipment and the pretreatment method, effective continuous production of biomass raw materials can be achieved, low-inhibitor lignocellulose hydrolysis sugar liquid is obtained, fermentation conversion of downstream microorganisms is facilitated, wastewater discharge and use of chemical auxiliaries are reduced, and commercialized large-scale production is expected to be further achieved.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

This invention discloses a strain that degrades various lignin-derived aromatic compounds and its application in detoxification of hydrolysate. The strain is classified as *Rhodococcus ethereans*. Rhodococcus ether-eating Strain N1, deposited at the China Center for Type Culture Collection (CCTCC) on August 11, 2022, with accession number CCTCC NO: M 20221270, can degrade p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillin, coniferyl alcohol, and syringaldehyde. After lignin depolymerization, derivative phenolic inhibitors are formed, mainly divided into three types: S-type (syringaldehyde), G-type (ferulic acid), and H-type (p-coumaric acid), which affect the fermentation of the strain. The Rhodococcus bacterium in this invention can efficiently degrade SGH-type monomers, achieving a removal efficiency of up to 68.4% when applied to the biological detoxification of hydrolysate inhibitors. Strain N1 has significant application value in improving the conversion efficiency of lignocellulose hydrolysate and reducing the detoxification cost in industrial production.

The application discloses a furaldehyde-tolerant Rhodotorula mucilaginosa strain and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is July 1, 2024, the preservation number is CGMCC NO.31122, and the Rhodotorula mucilaginosa strain is named Rhodotorula mucilaginosa F3.The furaldehyde concentration that can be tolerated by the Rhodotorula mucilaginosa F3 strain is 40mM-45mM, so that the production cost of carotenoids can be reduced, the utilization efficiency of lignocellulose hydrolysate is improved, waste utilization is realized, and good commercial and scientific research values are obtained.

The invention discloses an artificial multicellular system and application thereof, and belongs to the technical field of microorganisms. According to the method, the optimal inoculation ratio of the returning soil to the fresh cow dung is determined to be 1: 1, secondary screening of a bacterial system is carried out under the condition, an artificial multicellular system is obtained, the final accumulated total methane yield reaches 560.39 mL / (gVS) and the methane yield is 36.20 mL / (gVS.d) when the artificial multicellular system is used for fermentation, and the methane yield is improved by 34.17% compared with that of a primary generation. Compared with a single-environment inoculum, the yield of methane in a TF group is increased by 68.76% compared with T-P1 (primary-generation only returning-to-field soil) and is increased by 58.01% compared with F-P1 (primary-generation only fresh cow dung). According to the method, the lignocellulose hydrolysis efficiency and the methane conversion capacity are effectively improved, and a new way is provided for lignocellulose waste treatment and biological energy source production.

This invention discloses a method for promoting the hydrolysis of lignocellulose into fermentable sugars, belonging to the field of biorefining technology. The method includes the following steps: (1) adding ammonium sulfate to the lignocellulose raw material, then mixing it with a glycerol-water mixture, and pretreating it at a certain temperature and time to destroy the dense structure of the lignocellulose; (2) adding ammonium sulfate and cellulase to the pretreated system, and carrying out a saccharification reaction under suitable conditions to convert it into fermentable sugars. This invention solves the problems of high energy consumption, high pollution, and low saccharification and enzymatic hydrolysis efficiency of traditional pretreatment by leveraging the solubilizing, crystallinity-reducing, and biocatalyst-protecting effects of glycerol and the synergistic effect of ammonium sulfate. Compared with traditional hot water pretreatment, the yield of fermentable sugars is significantly increased, and the pretreatment process does not require strong acids or bases; glycerol can be recycled and reused, significantly reducing production costs and possessing significant industrial application value.

The invention belongs to the technical field of microorganism application, and relates to a corynebacterium glutamicum engineering bacterium for producing gamma-aminobutyric acid (GABA) by utilizing lignocellulose and application of the corynebacterium glutamicum engineering bacterium. A metabolic engineering means is adopted, a secretory expression type glutamate decarboxylase coding gene is integrated in a corynebacterium glutamicum genome, an isocitrate dehydrogenase coding gene and a GABA permease coding gene are knocked out, and the recombinant strain corynebacterium glutamicum GJ09 is obtained. The corynebacterium glutamicum engineering bacterium is the corynebacterium glutamicum engineering bacterium capable of producing gamma-aminobutyric acid by utilizing lignocellulose. The engineering bacterium has the metabolic capability on various biomass-derived sugars, especially lignocellulose-derived glucose and xylose, does not need to add antibiotics to maintain plasmids, and can stably produce gamma-aminobutyric acid in a lignocellulose hydrolysate system.

The invention relates to the technical field of microorganisms, and particularly discloses saccharomyces cerevisiae IMPHA-6 and application thereof in fermentation production of ethanol. The invention provides a Saccharomyces cerevisiae strain capable of resisting multiple stress, which is Saccharomyces cerevisiae IMPHA-6, and the preservation number of the Saccharomyces cerevisiae strain is CCTCC (China Center for Type Culture Collection) NO: M 20251412. The saccharomyces cerevisiae strain can solve the problem of low fermentation efficiency caused by a composite inhibitor (such as potassium chloride, ethanol, furfural and vanillin) in the lignocellulose hydrolysate, and can remarkably improve the reducing sugar utilization rate and ethanol yield during fermentation of the corn straw cellulose hydrolysate; a novel efficient microbial resource and method are provided for biomass energy production, and promotion of industrial production of cellulosic ethanol is facilitated.

The invention discloses a method for producing gamma-aminobutyric acid through double-bacterium synergistic fermentation of lignocellulose hydrolysate, and belongs to the technical field of microbial engineering. The method comprises the following steps: constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici FR-XY) engineering bacterium, and constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici) FR-XY; the method comprises the following steps: carrying out staged fermentation culture on lactobacillus hilgardii FR-1012 and recombinant pediococcus acidilactici FR-XY by utilizing lignocellulose hydrolysate, so as to obtain mixed fermentation liquor; centrifuging the mixed fermentation liquor, collecting mixed thalli, resuspending the mixed thalli in a conversion solution containing L-glutamic acid and coenzyme 5-phosphopyridoxal, and carrying out whole-cell conversion reaction to obtain a conversion solution containing gamma-aminobutyric acid. According to the method, the lignocellulose hydrolysate can be utilized for double-bacterium synergistic fermentation, and full utilization of a multi-element carbon source in the lignocellulose hydrolysate and efficient synthesis of gamma-aminobutyric acid are achieved.

The invention relates to the technical field of biology, and particularly discloses bacillus coagulans and application thereof in biosynthesis of L-lactic acid by using non-grain sugar. According to the invention, a high-temperature-resistant strain bacillus coagulans BC-A is obtained through breeding. The strain has broad-spectrum substrate adaptability, can efficiently utilize different sources of agricultural waste saccharification hydrolysate, can convert different mixed sugars composed of glucose / xylose into L-lactic acid, the sugar-acid conversion rate is over 96%, the yield of L-lactic acid reaches 90-200g / L, and the strain is suitable for industrial production. Therefore, the strain has remarkable advantages and wide industrial application prospects in the aspect of producing high-value L-lactic acid by using complex and cheap non-grain biomass raw materials (such as lignocellulose hydrolysate). Meanwhile, the strain is perfectly matched with the optimal temperature of trichoderma reesei cellulase, cellulose enzymolysis saccharification and lactic acid fermentation are synchronously completed in the same reaction system, and the efficiency limitation of a traditional step-by-step process is broken through.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to a yarrowia lipolytica engineering bacterium, a construction method thereof and application of the yarrowia lipolytica engineering bacterium in production of succinic acid. Specifically, by establishing a multi-copy expression method of xylose metabolic pathway genes XR, XDH and XK in yarrowia lipolytica, multi-copy integration and high-level expression of the genes are realized, and the constructed yarrowia lipolytica engineering bacteria can be used for preparing the yarrowia lipolytica engineering bacteria under the culture condition of taking xylose and lignocellulose hydrolysate as substrates, so that the yield of the yarrowia lipolytica engineering bacteria is increased, and the yield of the yarrowia lipolytica engineering bacteria is increased. Therefore, the method has a good practical application value.

The invention discloses a beta-xylosidase mutant derived from aspergillus brazilis, a gene, a recombinant plasmid, a modified cell and application, and relates to the technical field of biological genetic engineering. The beta-xylosidase mutant is subjected to amino acid mutation on the basis of a sequence as shown in SEQ ID NO: 1, and the amino acid mutation is single-point mutation or combined mutation of S175F, V293I, Q390E, A449S, N526G, Y565K and N576A. The beta-xylosidase is subjected to specific site combination mutation, so that the enzymatic activity and the thermal stability are synergistically improved, and the xylose yield is remarkably improved when the beta-xylosidase is compounded with xylanase for lignocellulose hydrolysis.

The application discloses a method for synchronously and efficiently metabolizing mixed sugar based on a Pichia pastoris mixed flora and application thereof. The Pichia pastoris mixed flora comprises at least one Pichia pastoris engineering strain for xylose metabolism and one Pichia pastoris engineering strain for oligosaccharide metabolism. By means of functional division of the strains, the interference of glucose signal on the xylose metabolic pathway is physically and genetically isolated, and the synchronous metabolism of xylose and cellulosic oligosaccharide is realized. The metabolic burden of each strain is reduced, the exclusive pathway of each strain can be optimized, the pathway competition and the imbalance of auxiliary factors are avoided, the overall fermentation rate and the sugar conversion rate are improved. The mixed flora system has a certain buffering capacity to environmental fluctuations and is more stable than a single strain. The strategy is particularly suitable for complex lignocellulose hydrolysate, and can effectively solve the problem of asynchronous utilization of multiple fermentable sugars in the lignocellulose hydrolysate and improve the total utilization rate of raw materials. In the process of simultaneous saccharification and fermentation of the mixed flora, the utilization rate of xylose and cellulosic oligosaccharide is improved, and the integrated biorefinery cycle is shortened.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

Disclosed herein are feedstocks and methods of culturing A. pullulans for heavy oil production. A method of producing heavy oil can include providing one or more feedstocks comprising deacetylated and mechanically refined lignocellulosic hydrolysate media and one or more nutritional carbon sources, culturing a cell population comprising A. pullulans, or a genetically modified version thereof, on the one or more feedstocks, and generating the heavy oil using the cell population.

The present invention discloses a cold-resistant composite bacterial agent for decomposing rice straw at low temperature and its application, belonging to the field of microbial technology. It is composed of Flavobacterium F1, Flavobacterium F2, Flavobacterium F3, Oxalobacterium 01, Oligotrophomonas S1 and Pseudomonas P1. There is no obvious antagonistic inhibition between the strains of the cold-resistant composite bacterial agent, and each strain has the ability to produce cellulase and filter paper enzyme under low temperature environment, among which the cellulase activity reaches up to 16.48U / mL, and the filter paper enzyme activity reaches up to 21.71U / mL. When the composite bacterial agent is used to degrade rice straw at low temperature, it can significantly reduce the mechanical strength of the straw and significantly reduce the cellulose, hemicellulose and lignin content in the straw. Under low temperature conditions, the cold-resistant composite bacterial agent can stably produce lignocellulose hydrolyzing enzymes, especially cellulase, accelerate the decomposition of rice straw and the release of nutrients, and has good development and application prospects.

The invention discloses a process for separating sugar and acid by a three-zone simulated moving bed. The three-zone simulated moving bed is formed by more than four resin columns filled with H-type cation exchange resin in a looping manner, every 1-6 resin columns form one zone, and the three zones are sequentially an adsorption separation zone III, an acid enrichment zone II and a desorption regeneration zone I; the resin columns in the three areas are respectively connected in series, more than two resin columns are arranged in the adsorption separation area III, and the resin columns comprise a first resin column and a second resin column which are connected in series; an outlet of the desorption regeneration area I is communicated with an inlet of the acid enrichment area II, and an outlet of the acid enrichment area II and an outlet of the first resin column in the adsorption separation area III are connected to an inlet of the second resin column in parallel; the three-zone simulated moving bed is used for separating sugar and acid in the lignocellulose hydrolysis sugar liquid containing the inorganic acid.

The present invention discloses xylose isomerases obtained based on an ancestral sequence construction method and their applications. These xylose isomerases are obtained based on the ancestral sequence construction method and have any of the amino acid sequences ID NO. 1 to SEQ ID NO. 4. Expression of these xylose isomerases alone or in combination can confer upon yeast cells the ability to convert xylose to xylulose, thereby conferring upon host cells the ability to convert xylose to other products. The present invention also relates to the application of these four xylose isomerases in yeast production of chemicals such as ethanol using xylose as a substrate. When expressed in yeast cells such as Saccharomyces cerevisiae, these xylose isomerases can confer upon a host cell that originally lacks the ability to convert xylose to xylulose this ability, conferring upon the host cell the ability to produce chemicals such as ethanol using xylose or lignocellulose hydrolysate, which are rich in xylose.

The application discloses a method for preparing functional humus by using nano-iron composite catalyst to induce directional humification of straw and application thereof, and belongs to the technical field of agricultural solid waste resource utilization and humus synthesis. The method uses agricultural straw as raw material, improves the reaction activity through alkali pre-dissociation, and then uses ternary nano-iron composite catalyst composed of nano-Fe2O3, zero-valent nano-iron and nano-FeCl3 to perform directional hydrothermal humification, so as to induce directional condensation of straw lignocellulose hydrolysis products and enrich acid-containing oxygen and nitrogen functional groups. After the reaction, solid-liquid separation is performed and differential modification is performed, the solid phase is used to prepare nano-iron-based hydrothermal humic carbon for saline-alkali farmland improvement, and the liquid phase is used to prepare functional humic acid foliar fertilizer for crop growth promotion. The application shortens the reaction period to 4 hours, realizes directional regulation of functional groups and high-value utilization of all components of straw, and realizes synchronous improvement of soil fertility and yield increase of soybeans and corn through a matched regional application process.