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Method for producing microbial oil by fermenting corn stalk hydrolysate

A technology for microbial oil and corn stalks, which is applied to the field of microbial oil and the production of microbial oil by fermentation of corn stalk hydrolyzate, can solve the problems of low yield, complicated methods, etc. Effect

Inactive Publication Date: 2012-10-10
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are also some methods for preparing microbial oil, but the general yield is low and the method is relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for producing microbial oil by fermenting corn stalk hydrolyzate, the specific steps are as follows:

[0041] ⑴ Preparation of corn stalk hydrolyzate

[0042] After the corn stalks were cleaned, dried, and crushed, they were passed through a 40-mesh sieve, and a dilute sulfuric acid solution with a mass percentage of 2% was added according to the solid-to-liquid ratio (g:mL) of the corn stalk powder and the dilute acid solution (1:8). High-pressure hydrolysis at 121°C for 20 minutes, and centrifugation at 3800r / min for 15 minutes to obtain corn stalk hydrolyzate, i.e. lignocellulose hydrolyzate;

[0043] The content of reducing sugar in the hydrolyzate was measured by DNS method;

[0044] ⑵Detoxification treatment

[0045] Ca(OH) 2 Detoxification treatment: adding Ca(OH) to the hydrolyzate 2 Powder, adjust the pH to 10-11, centrifuge at 3800r / min for 10min, take the supernatant, adjust the pH to 6.0 with phosphoric acid, centrifuge at 3800r / min for 10min, t...

Embodiment 2

[0058] A method for producing microbial oil by fermenting corn stalk hydrolyzate, the specific steps are as follows:

[0059] ⑴ Preparation of corn stalk hydrolyzate

[0060] After the corn stalks were cleaned, dried, and crushed, they were passed through a 40-mesh sieve, and a dilute sulfuric acid solution with a mass percentage of 2% was added according to the solid-to-liquid ratio (g:mL) of the corn stalk powder to the dilute acid solution (g:mL) of 1:10. High-pressure hydrolysis at 121°C for 20 minutes, and centrifugation at 3800r / min for 15 minutes to obtain corn stalk hydrolyzate, i.e. lignocellulose hydrolyzate;

[0061] The content of reducing sugar in the hydrolyzate was measured by DNS method;

[0062] ⑵Detoxification treatment

[0063] Ca(OH) 2 Detoxification treatment: adding Ca(OH) to the hydrolyzate 2 powder, adjust the pH to 10-11, centrifuge at 3800r / min for 15min, take the supernatant, adjust the pH to 6.0 with phosphoric acid, centrifuge at 3800r / min for ...

Embodiment 3

[0076]A method for producing biodiesel by fermenting corn stalk hydrolyzate, the specific steps are as follows:

[0077] ⑴ Preparation of corn stalk hydrolyzate

[0078] After the corn stalks were cleaned, dried, and crushed, they were passed through a 40-mesh sieve, and a dilute sulfuric acid solution with a mass percentage of 2% was added according to the solid-to-liquid ratio (g:mL) of the corn stalk powder to the dilute acid solution (g:mL) of 1:10. High-pressure hydrolysis at 121°C for 20 minutes, and centrifugation at 3800r / min for 15 minutes to obtain corn stalk hydrolyzate, i.e. lignocellulose hydrolyzate;

[0079] The content of reducing sugar in the hydrolyzate was measured by DNS method;

[0080] ⑵Detoxification treatment

[0081] Ca(OH) 2 Detoxification treatment: adding Ca(OH) to the hydrolyzate 2 Powder, adjust the pH to 10-11, centrifuge at 3800r / min for 20min, take the supernatant, adjust the pH to 6.0 with phosphoric acid, centrifuge at 3800r / min for 15min...

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Abstract

The invention relates to a method for producing a microbial oil by fermenting corn stalk hydrolysate. The method comprises the following steps: (1) hydrolyzing corn stalks with acid to obtain the corn stalk hydrolysate; (2) adding Ca(OH)2 into the hydrolysate, regulating the pH to 10 to 11, centrifuging the solution for 10 to 20 min at a speed of 3,800 r / min, taking the supernatant, regulating the pH back to 6.0 with phosphoric acid, centrifuging the solution for 10 to 20 min at a speed of 3,800 r / min, taking the supernatant, sterilizing and filtering the supernatant, and regulating the pH of the obtained filtrate with NaOH to 6.0; (3)fermenting and cultivating oleaginous microorganism; and (4)crushing and extracting the dried bacteria in step (3) to obtain the microbial oil. According to the method for producing the microbial oil, the corn stalks are hydrolyzed by dilute sulphuric acid to obtain lignocellulosic hydrolysate, which is detoxified by Ca(OH)2 to effectively reduce or inhibit the fermentation inhibitor in the hydrolysate, so as to promote the fermentation of the microorganism and obtain a large amount of microbial oils which can be further transformed into biodiesel.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to microbial oil, in particular to a method for producing microbial oil by fermenting corn stalk hydrolyzate. Background technique [0002] At present, energy and environment issues are attracting more and more people's attention, and have become one of the main problems faced by countries all over the world. How to deal with the contradiction between the increasing demand for fossil energy and the serious shortage of natural resources and realize sustainable development is the focus of researchers from all over the world. As a green renewable energy that can replace traditional fossil fuels, biodiesel has great potential for development. Biodiesel is a kind of biomass energy, which is made from oil crops, wild oil plants, engineering microalgae and other aquatic plant oils, animal oils, and waste oil from catering, etc., through transesterification process. Regenerative diesel fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCY02E50/13Y02E50/10
Inventor 刘泽君罗成
Owner TIANJIN UNIV OF SCI & TECH
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