Method of producing yeast biomass

a technology of saccharomyces yeast and biomass, which is applied in the field of producing saccharomyces yeast, can solve the problems of unsuitable growth of waste streams, and achieve the effect of reducing the biological oxygen demand of a substra

Inactive Publication Date: 2011-07-28
MICROBIOGEN PTY LTD
View PDF2 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0122]A fifteenth aspect provides a method of reducing the biological oxygen demand of a substrate comprising C5 compound-containing material obtained from ferment

Problems solved by technology

In particular, C5 compound-containing material obtained from fermentation of lignocellulos

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of producing yeast biomass
  • Method of producing yeast biomass
  • Method of producing yeast biomass

Examples

Experimental program
Comparison scheme
Effect test

example 1

Growth of Saccharomyces Strain NMI V08 / 013,411 and Saccharomyces Strain V09 / 005,064 Using Xylose as a Sole Carbon Source in Minimal Mineral Medium

[0166]Saccharomyces cerevisiae strain V08 / 013,411 was deposited at the National Measurement Institute, 51-65 Clarke Street, South Melbourne, Victoria, 3205, Australia under the Budapest Treaty on 23 May 2008 under accession number V08 / 013,411.

[0167]Saccharomyces cerevisiae strain V09 / 005,064 was deposited at the National Measurement Institute, 1 / 153 Bertie Street, Port Melbourne, Victoria, 3207, Australia, under the Budapest Treaty on 18 Feb. 2009 under accession number V09 / 005,064.

[0168]Saccharomyces yeast strain ER, which is representative of Saccharomyces yeast used for industrial starch-to-ethanol production, was isolated using standard microbiological procedures from a pack of “Ethanol Red” dry alcohol yeast which is commercially available from Fermentis, BP 3029-137 rue Gabriel Péri, F-59703 Marcq-en-Baroeul Cedex France (batch numbe...

example 2

Preparation of Lignocellulosic Hydrolysates

[0170]Washed lignocellulosic biomass was dried to <5% moisture at 90-120° C. and reduced to <1.5 mm using a hammer-mill to produce dry milled biomass.

[0171]Sulphuric acid was mixed at a level of 55.2 mg sulphuric acid per gram of dry biomass, into the dry milled biomass in order to produce a 25% w / w solid / water mash.

[0172]The acidified solid / water mash was transferred to an autoclavable plastic drum, covered with aluminium foil and placed within a 100 L pressure vessel. The temperature of the pressure vessel was maintained at 130 degrees C., 23 psi / 160 kPa for 90 minutes. The vessel was then depressurised to allow atmospheric pressure to be reached within 3 minutes.

[0173]The resulting hydrolyzed mash was fed into a manual screw press and dewatered. The moisture content of the pressed mash was 60-65%. Hydrolysate is liquid from the hydrolysed mash.

[0174]The hot liquor (the ‘hydrolysate’) had a pH of 0.8 to 1.5, and was continuously stirred. ...

example 3

High Density Inoculation of Lignocellulosic Hydrolysate Facilitates Rapid Fermentation

[0179]Saccharomyces cerevisiae strains ER and NMI V08 / 013,411 were grown in sucrose-minimal medium and harvested as described in Myers et al. (1997) Applied and Environmental Microbiology, 63: 145-150, to provide yeast biomass for inoculum.

[0180]One hundred milliliters of pH adjusted hydrolyzate liquor was buffered by addition of 1% tri-sodium citrate to pH 5.0. Citrate buffering was used to help maintain subsequent culture pH within a suitable range for yeast growth. pH control in culturing and fermentation could also be achieved by titration with other common acids and bases. Sixteen percent (weight per volume) D-glucose was dissolved into the hydrolyzate liquor for conversion to ethanol. The glucose was added to the hydrolyzate liquor in order to mimic the hexoses that would have been available for yeast to utilize if hydrolysed cellulose or other hexose-rich materials were employed. The buffere...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to use of a substrate comprising C5 compound-containing material, in the growth of Saccharomyces yeast or the production of a product of Saccharomyces yeast, wherein the C5 compound-containing material is: (a) C5 compound-containing material obtained from lignocellulosic hydrolysate; (b) C5 compound-containing material obtained from fermentation of lignocellulosic hydrolysate; or (c) a mixture of (a) and (b). The invention also relates to a method of producing Saccharomyces yeast biomass or a product of Saccharomyces yeast using the substrate, and to methods of producing ethanol comprising incubating Saccharomyces yeast produced by the use or method. The invention further relates to strains of Saccharomyces yeast suitable for the use or methods.

Description

FIELD[0001]The invention relates to a method of producing Saccharomyces yeast biomass or a product of a Saccharomyces yeast, substrates for growth of such yeast, to strains of Saccharomyces yeast, and use of the strains of Saccharomyces yeast in the production of yeast biomass, and yeast products such as ethanol. The invention has particular application for producing ethanol using Saccharomyces yeast and the invention is herein described in that context.BACKGROUND[0002]Ethanol is an increasingly important renewable fuel for transport, as well as being important as an industrial chemical and 2-carbon chemicals precursor. Ethanol is mostly produced via fermentation of hexose based sugars by Saccharomyces yeast. The hexose based sugars are typically obtained from crop plants in the form of hydrolyzed starches from corn, wheat, barley, sorghum, millet, cassava, sweet potato, rice etc., or from sugar cane and sugar beet. The hexose based sugars that are fermented are typically glucose, f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/06C12P1/02C12N1/16
CPCC12N1/18C12N1/22Y02E50/17C12R1/865Y02E50/16C12P7/10Y02E50/10C12R2001/865C12N1/185C12P7/06C12N1/16
Inventor BELL, PHILIP JOHN LIVINGSTONEATTFIELD, PAUL VICTORKOLLARAS, ARTHUR
Owner MICROBIOGEN PTY LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap