29 results about "Cellulose hydrolysis" patented technology

The invention relates to the technical field of biology, in particular to pseudomonas fluorescens GS-1 for synthesizing polyhydroxyalkanoate and application of the pseudomonas fluorescens GS-1, the pseudomonas fluorescens GS-1 is preserved in China General Microbiological Culture Collection Center on February 6, 2026, and the preservation number is CGMCC No.37739; the strain carries a phaC gene and can synthesize PHA, and the straw sugar PHA conversion efficiency reaches 87.7% of that of a glucose system; the method has inherent lignocellulose hydrolysate inhibitor tolerance, and the PHA synthesis capability retention rate in the complex self-made corn straw saccharification liquid reaches 91.4%. A high-quality strain resource with direct application potential is provided for producing the PHA based on cheap straw saccharification liquid.

The invention discloses a method for selectively preparing C6 ketone and 1, 2-hexanediol by accelerating cellulose hydrolysis based on a crude hydrogen atmosphere. The method comprises the following steps: by taking cellulose as a raw material, water as a solvent and activated carbon supported Pt as a catalyst, reacting for 1-6 hours under the conditions that the total pressure of hydrogen source gas containing CO and CO2 is 0.5-4.0 MPa and the temperature is 210-260 DEG C, and accelerating cellulose hydrolysis to obtain a product mainly comprising a C6 ketone compound and 1, 2-hexanediol; the catalyst is obtained by adding a pretreated carbon carrier into an ethanol solution, adding a platinum salt precursor solution, sealing, carrying out a hydrothermal reaction, carrying out suction filtration, washing and drying. According to the invention, the yield of C6 ketones is up to 53.4%, and the yield of 1, 2-hexanediol is up to 15.6%.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

This invention discloses a mesoporous silica-based composite material with phosphotungstic acid simultaneously immobilized with titanium oxide and its cellulose hydrolysis method, belonging to the field of biomass catalytic conversion technology. This invention aims to solve the problems of easy desorption of active components, low catalytic efficiency, and poor cycle stability in existing solid acid catalysts during cellulose hydrolysis. This method uses an ionic liquid as the reaction medium and a titanium-silicon-supported phosphotungstic acid composite material with a two-dimensional hexagonal ordered mesoporous structure prepared by a one-step hydrothermal synthesis as the solid acid catalyst, catalyzing the hydrolysis of cellulose to reduce sugars in the presence of water. This invention achieves highly efficient cellulose conversion through the synergistic catalytic effect of the two acid sites combined with the good solubility of cellulose in the ionic liquid. The catalyst exhibits excellent recyclability, the process conditions are well-defined, it meets the requirements of green chemistry, and it is applicable to the field of biomass resource utilization.

The invention discloses difunctional xylanase as well as a preparation method and application thereof. According to the bifunctional xylanase, a C-terminal Dockeren structural domain is knocked out on the basis of wild type xylanase Xyn1G3 as shown in an amino acid sequence SEQ ID NO. 1, and the amino acid sequence of the bifunctional xylanase comprises a sequence as shown in SEQ ID NO. 3. A novel bifunctional enzyme with a simplified structure and optimized functions is successfully constructed by directional knockout of a Dockeren structural domain through a genetic engineering means, the bifunctional xylanase not only retains the original activity of xylanase, but also shows remarkable activity of cellulose hydrolase, and the application potential of the bifunctional xylanase in biomass degradation is expanded.

The invention relates to a recombinant halomonas, which comprises an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), so that the copolymer can be synthesized through cellulose hydrolysate containing xylose, the production cost is reduced, and a new thought is provided for green manufacturing.

This invention discloses a reagent kit and method for the joint detection of glucose, cellobiose, and xylose in cellulose hydrolysate, belonging to the field of biomass conversion and analytical detection technology. The method includes the following steps: setting up three types of detection channels: a glucose oxidase detection channel that specifically responds to the glucose component; a β-glucosidase-glucose oxidase combined detection channel that responds to the total signal of glucose and cellobiose; and a pyranose oxidase detection channel that simultaneously responds to the combined signals of glucose, cellobiose, and xylose. Enzymatic colorimetric reactions are performed on the cellulose hydrolysate sample and the multi-component gradient standard solution in the three types of detection channels, respectively. A ternary linear calibration model is constructed based on the absorbance data of the three types of channels of the multi-component gradient standard solution. This method provides a rapid, low-cost, and highly specific detection method for glucose, cellobiose, and xylose in cellulose biomass hydrolysate, and has practical application value.

This invention discloses a genetically engineered bacterium for producing rhamnolipids and its applications, belonging to the field of gene recombination and metabolic engineering technology. The genetically engineered bacterium for producing rhamnolipids disclosed in this invention uses *Yarrowia lipolyticis* ATCC MYA-2613 as the starting strain and heterologously expresses the rhamnolipid synthesis gene. rhlA, rhlB and rhlC, and dTDP-L-rhamnose synthesis gene rmlA, rmlB, rmlC and rmlD This invention discloses a genetically engineered bacterium for producing rhamnolipin, which can efficiently metabolize glucose or glucose-containing cellulose hydrolysates to produce rhamnolipin, achieving a rhamnolipin yield of 42.8 g / L. The genetically engineered bacterium constructed in this invention provides a possibility for the efficient production of rhamnolipin from glucose or glucose-containing cellulose hydrolysates.

The invention discloses an acid-metal bifunctional catalyst for catalyzing cellulose to prepare isosorbide as well as a preparation method and application of the acid-metal bifunctional catalyst, the catalyst takes an acid-functionalized porous organic polymer as a carrier to load metal nanoparticles, the chemical general formula of the catalyst is M-PPh3-SO3H (at) POPs, and M is Ru, Pt, Pd or Ni. According to the catalyst, an acid active center is provided through a sulfonic acid group and used for cellulose hydrolysis and sorbitol dehydration, meanwhile, a hydrogenation center is provided through metal nanoparticles and used for glucose hydrogenation, and synergistic catalysis of acid-metal double active centers is achieved. The invention also provides a preparation method of the catalyst. The preparation method comprises the steps of carrier synthesis, acid functionalization and metal loading. The catalyst is applied to a reaction for directly preparing isosorbide from cellulose, the technological process can be remarkably simplified, the yield of isosorbide can reach 85% or above under the optimal condition, the catalyst is good in stability, and the activity retention rate exceeds 90% after the catalyst is repeatedly used five times.

The invention discloses a method for producing gamma-aminobutyric acid through double-bacterium synergistic fermentation of lignocellulose hydrolysate, and belongs to the technical field of microbial engineering. The method comprises the following steps: constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici FR-XY) engineering bacterium, and constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici) FR-XY; the method comprises the following steps: carrying out staged fermentation culture on lactobacillus hilgardii FR-1012 and recombinant pediococcus acidilactici FR-XY by utilizing lignocellulose hydrolysate, so as to obtain mixed fermentation liquor; centrifuging the mixed fermentation liquor, collecting mixed thalli, resuspending the mixed thalli in a conversion solution containing L-glutamic acid and coenzyme 5-phosphopyridoxal, and carrying out whole-cell conversion reaction to obtain a conversion solution containing gamma-aminobutyric acid. According to the method, the lignocellulose hydrolysate can be utilized for double-bacterium synergistic fermentation, and full utilization of a multi-element carbon source in the lignocellulose hydrolysate and efficient synthesis of gamma-aminobutyric acid are achieved.

This application relates to the technical field of *Fermentomonas motilityis*, specifically to engineered strains of *Fermentomonas motilityis*, complex proteins, and methods. The engineered strain is *Fermentomonas motilityis* containing genes encoding exoglucanase, endoglucanase, β-glucosidase, coliformin E protein, and a protein that interacts with the coliformin E protein. This engineered strain can efficiently express exoglucanase, endoglucanase, β-glucosidase, coliformin E protein, and the protein that interacts with the coliformin E protein, and facilitates the formation of complex enzyme assemblies, thereby increasing the enzyme activity and glucose yield in cellulose hydrolysis, and reducing the energy consumption of cellulose hydrolysis, thus possessing significant industrial application value.

The application discloses chitooligosaccharide from squid cartilage, and a preparation method and application thereof, and belongs to the technical field of amino polysaccharide extraction and application. The chitooligosaccharide is prepared from chitin obtained by desalting and deproteinization of squid cartilage, and a deproteinization process for obtaining the chitin is also provided. The chitin is prepared into beta-chitosan through deacetylation, and the beta-chitosan is prepared into chitooligosaccharide through cellulose hydrolysis. The preparation process of the chitooligosaccharide is simple and easy to implement. The characterization information of the prepared chitooligosaccharide is analyzed through experiments, the chitooligosaccharide contains oligomers with a polymerization degree of 2-6, and is a positively charged oligosaccharide. The application also provides application of the chitooligosaccharide in treatment and relief of type II diabetes. A large number of experiments prove that the chitooligosaccharide from squid cartilage has biological functions such as antioxidant, anti-diabetic, cholesterol-lowering, immune regulation and antibacterial activity, the prepared chitooligosaccharide from squid cartilage has relatively comprehensive biological functions, the preparation process is simple, and the chitooligosaccharide has wide application value in medicines and foods.

The invention belongs to the technical field of epsilon-polylysine production, and particularly relates to a screening culture method and application of tolerant streptomyces hygroscopicus. The screening culture method of the tolerant streptomyces hygroscopicus comprises the following steps: subculturing streptomyces hygroscopicus engineering bacteria in a culture medium taking straw cellulose hydrolysate as a carbon source for at least 30 generations to obtain primary streptomyces hygroscopicus. The primary streptomyces hygroscopicus is subcultured for 10-25 generations in a fermentation culture medium with the epsilon-polylysine concentration being 0.5 g / L-5g / L and straw cellulose hydrolysate as a carbon source, and the tolerant streptomyces hygroscopicus is obtained. Wherein the tolerance means that epsilon-polylysine is produced while an inhibitor is resisted. The straw cellulose hydrolysate contains an inhibitor. According to the screening culture method of the tolerant streptomyces hygroscopicus, disclosed by the invention, the yield of epsilon-polylysine in virus-free cellulose hydrolysate can be up to 1.35 g / L at most, and the yield of epsilon-polylysine can be up to 30.57 g / L at most under the condition of fed-batch fermentation in a 5L fermentation tank.

This invention provides a method for preparing a bifunctional mesoporous solid acid catalyst and its application in the hydrolysis of cellulose to produce glucose. Using alkali lignin as the carbon source and sucralose as the chlorine source, the bifunctional mesoporous solid acid catalyst is obtained after high-temperature carbonization and concentrated sulfuric acid sulfonation. This catalyst not only possesses a high density of -SO3H groups, enabling efficient cleavage of cellulose glycosidic bonds, but also exhibits -Cl groups that can form hydrogen bonds with cellulose, enhancing the catalyst's adsorption capacity for cellulose polysaccharides. When the prepared catalyst is used for cellulose hydrolysis, it can efficiently convert cellulose into glucose at a temperature of 150–190 °C for 2–6 h. It exhibits advantages such as high catalytic efficiency, few byproducts, and low corrosiveness to equipment, making it an environmentally friendly solid acid catalyst.

The invention discloses an engineering strain for efficiently producing Ectoine through polysaccharide co-utilization and a fermentation process, and belongs to the technical field of gene recombination and metabolic engineering. According to the invention, crr, ptsG and overexpression genes of lysC, glk, galp, xylA, xylB, xylR, ectA, ectB and ectC are knocked out, so that recombinant escherichia coli is obtained. According to the escherichia coli disclosed by the invention, glucose and xylose can be efficiently metabolized at the same time, the yield of Ectocaine can reach 162.2 g / L when cellulose hydrolysate from straw is used, the yield reaches 0.37 g / g carbon source, and xylose is efficiently utilized. The engineering Escherichia coli constructed by the invention provides a powerful basis for producing Ectoin by efficiently utilizing cheap carbon sources such as cellulose hydrolysate and the like.

The invention relates to the technical field of cooked wheaten food preparation, in particular to a method for preparing cooked wheaten food and pastry by using a coupled cell wall breaking method, which comprises the following steps: cleaning, dicing, pulping, homogenizing and performing ultrasonic wall breaking treatment on fresh fruits and vegetables rich in cellulose hydrolase and plant lytic enzyme to obtain a flour wall breaking coupling agent; the preparation method comprises the following steps: taking flour in parts by weight, coupling, mixing and stirring uniformly, kneading into dough, fermenting for 20-200 minutes at the temperature of 40-45 DEG C, and processing into cooked wheaten foods and pastries with different structures and shapes by using a cooked wheaten food processing machine. Cellulose hydrolase and plant clastic enzyme in fruits and vegetables can be fully released and activated, then flour cell walls are efficiently decomposed, release of nutritional ingredients such as starch and protein is promoted, and the digestion and absorption effects of human bodies on the nutrients are improved.

The invention discloses saccharomyces cerevisiae capable of efficiently utilizing cellulose hydrolysate as well as a construction method and application thereof. The construction method comprises the following steps: 1) selecting a xylose metabolism saccharomyces cerevisiae haploid chassis strain of a xylose isomerase pathway; 2) performing spore splitting on industrial ethanol-producing yeast to obtain haploid industrial yeast strains; and (3) fusing and screening the strains obtained in the steps (1) and (2) to obtain the saccharomyces cerevisiae capable of efficiently utilizing the cellulose hydrolysate. The saccharomyces cerevisiae. At the shake flask level, 70 g / L of glucose in the acid-treated cellulose hydrolysate can be completely consumed within about 24 h, meanwhile, xylose starts to be consumed gradually, and 20 g / L of xylose in the acid-treated cellulose hydrolysate can be consumed within 72 h. The growth amount OD600 can reach about 15.0 at most, 45 g / L of ethanol is generated under the aerobic condition, rapid growth in industrial cellulose hydrolysate is achieved, glucose and xylose are fully utilized as growth carbon sources, and ethanol is generated.

The invention relates to a device (1) for cellulose hydrolysis. The device (1) comprises a channel (10) for guiding a cellulose-containing waste stream in a flow direction (FD). The device (1) further comprises one, two or more shafts (20) arranged to rotate about a respective longitudinal axis (A) such that, when rotated, one or more side surfaces (71) of said shafts (20) exhibit a linear velocity vector orthogonal to said axis (A). The two or more shafts (20) comprise one or more protrusions (70) extending radially outward from the axis (A). The invention also provides a method for cellulose hydrolysis.

The invention belongs to the technical field of natural product extraction and plant essential oil preparation, and particularly relates to wormwood essential oil prepared through a biological enzyme assisted steam distillation method and an extraction method thereof.The wormwood essential oil is obtained through steam distillation after double-enzyme compounding segmented gradient enzymolysis pretreatment and mainly comprises 28%-42% of cineole, 12%-18% of camphor and 8%-15% of alpha-platycladone. The extraction method comprises raw material pretreatment, first-stage cellulose enzymolysis (30-35 DEG C, pH 4.5-5.0, 2-3 h), second-stage pectin enzymolysis (38-42 DEG C, pH 5.0-5.5, 2-3 h), steam distillation (parameter linkage regulation and sectional distillation), oil-water separation and refining and cyclic utilization closed loop process, and compared with the prior art, the extraction rate of the essential oil is improved by 35-50%.

The invention discloses an ROS (reactive oxygen species) biosensor based on an OxyR mutant as well as a construction method and application of the ROS biosensor. According to the invention, point mutation from the 267th serine to the proline (S267P) is carried out on an oxidative stress key regulatory factor OxyR, so that the stress resistance of the strain to acetic acid, furfural, ethanol, hydrogen peroxide and the like is obviously enhanced on the premise of keeping the integrity of a regulatory network. Furthermore, the mutant is used as a sensing core, and is combined with a specific response promoter P1732 or P1753 and a reporter gene eGFP to construct a genetic coding type ROS biosensor. The sensor can quantitatively monitor the change of the active oxygen level caused by the inhibitor in living cells in real time and in situ, and the response strictly depends on the OxyRS267P mutant. The sensor has the dual functions of enhancing cell tolerance and realizing process monitoring, and provides a complete solution from stress-resistant strain construction to intelligent monitoring for microbial industrial fermentation, especially for the efficient conversion process of complex substrates such as cellulose hydrolysate.

The invention provides a cat prescription food for improving lower urinary tract diseases and a preparation method thereof, and the cat prescription food for improving urinary tract calculi comprises the following components in percentage by mass: a grain-free hypoallergenic raw material, chicken oil, yolk powder, chicken liver powder, fish oil, a Chinese herbal medicine raw material, cellulose, casein hydrolysate and 1-10% of a premix. By using the cereal-free low-sensitivity raw materials, the health of intestinal tracts can be cared; by adjusting the proportion of calcium, phosphorus, magnesium and other minerals, mineral balance of urine can be maintained, and by matching with herbaceous plant ingredients such as semen plantaginis, urination is promoted, and the calculus risk is reduced; by adding citrate, the combination of citrate and calcium ions is promoted, a soluble calcium citrate compound is formed, and the crystallization and deposition of calcium oxalate can be reduced; by controlling the protein content and adding the acidifying agent, weak acid urine can be maintained, bacterial growth can be inhibited, and struvite crystal dissolution can be promoted.

The invention belongs to the field of gene engineering, and discloses clostridium tyrobutyricum for producing butyl butyrate by jointly utilizing glucose and xylose as well as a construction method and application of the clostridium tyrobutyricum. The strain has the following characteristics: a restriction enzyme rease gene is knocked out, a lactic dehydrogenase ldh2 gene, a lactic dehydrogenase ldh3 gene and a lactic dehydrogenase ldh1 gene are knocked out, an HPr kinase / phosphorylase hprK gene is knocked out, an aldol dehydrogenase adhE2 gene is knocked in at the site, a xylose transcription inhibition factor xylR gene is knocked out, and an aldol dehydrogenase adhE2 gene is knocked in at the site; a butyryl-CoA / acetic acid CoA transferase cat1 gene is knocked out, an aldehyde alcohol dehydrogenase adhE2 gene is knocked into the site, and alcohol acyltransferase VAAT and heat shock protease GroESL are overexpressed. The strain is obtained by a gene editing method, and can utilize glucose and xylose in lignocellulose hydrolysate to produce butyl butyrate at high yield.

The present application relates to the technical field of deep processing of agricultural products, in particular to a preparation method of food-grade wheat straw superfine dietary fiber powder and application thereof, the preparation method comprising: raw material pretreatment, high-temperature and high-pressure cooking delignification, food-grade hot bleaching, neutralization and water washing, enzymatic hydrolysis softening, dehydration and drying, and ultrafine grinding.The beneficial effects are: the present application realizes complete removal of lignin and wax and superfine refinement of fiber by accurately controlling process parameters such as food-grade NaOH dosage, high-temperature and high-pressure cooking time, food-grade H2O2 bleaching, and cellulose hydrolysis.The dietary fiber powder prepared by the present application has a fineness of 200-500 meshes, a dietary fiber content of greater than or equal to 80%, a neutral pH, no grassy odor and bitter taste, and a delicate taste, and can directly replace 10-30% of flour for use in steamed buns, noodles, and other flour products.The present application has a simple process, can be industrialized for large-scale production, realizes high-value utilization of wheat straw, and has significant environmental and economic benefits.

The invention provides a system and a method for continuously catalyzing hydrolysis of cellulose into sugar. The invention relates to a method for continuously catalyzing hydrolysis of cellulose into sugar, which comprises the following steps: continuously conveying slurry containing solid acid and cellulose into a reactor, and hydrolyzing at the temperature of 100-250 DEG C under the condition of microwave irradiation to obtain hydrolysate; wherein the retention time of the slurry in the reactor is 1-60 minutes; and continuously outputting the hydrolysate from the reactor, and cooling until the temperature is less than or equal to 50 DEG C. According to the method, the slurry is subjected to high-power microwave irradiation coupling and synchronous cooling, cellulose in the slurry is rapidly depolymerized through the thermal effect and the non-thermal effect of microwaves, the cellulose is hydrolyzed into sugar, hydrolysate containing sugar products is obtained, meanwhile, the overall temperature of the hydrolysate is reduced, and side reactions such as sugar degradation are inhibited; meanwhile, rapid depolymerization of cellulose and selectivity of sugar are realized.

The invention provides a method and a system for catalyzing hydrolysis of cellulose into soluble sugar by a micro-channel continuous flow method. The method comprises the following steps: providing reaction slurry, wherein the reaction slurry contains cellulose particles and solid catalytic particles; enabling the reaction slurry to be in a micro-channel formed between the first limiting body and the second limiting body by a space confinement effect, raising the temperature, and carrying out cellulose hydrolysis reaction to obtain soluble sugar; the distance between the first limiting body and the second limiting body is in a millimeter level, and relative sliding motion is generated between the first limiting body and the second limiting body. According to the method and system provided by the invention, a micro-channel is formed by utilizing a space confinement effect, and relative slippage is generated to accelerate a mass transfer process, so that the problem of slow mass transfer between solid interfaces is greatly avoided, the rigid and compact molecular aggregation structure of cellulose is accelerated to be broken, the cellulose is excited to be efficiently hydrolyzed into soluble sugar, and the response capability is improved; the catalytic hydrolysis sugar forming speed is obviously increased, and the requirements of large-scale industrial application are met.

An example method of hydrolyzing cellulose includes: providing cellulose to be hydrolyzed; applying a stabilizing salt to the cellulose to form a reaction mixture, the stabilizing salt configured to suppress recrystallization of amorphous cellulose in the reaction mixture; and hydrolyzing the amorphous cellulose in the presence of the stabilizing salt to suppress recrystallization of the amorphous cellulose in the reaction mixture during hydrolysis.

The invention discloses a cellulase mutant with high enzyme activity and application thereof, glutamine at the 216th site of an amino acid sequence of wild STCE1 is subjected to site-specific mutagenesis to be changed into any one of glycine, serine, threonine and alanine, and a corresponding mutant gene, amino acid, a recombinant vector and a recombinant strain are obtained. And expressing to obtain the cellulase mutant. According to the invention, mutation sites of the STCE1 gene are enriched, the enzyme activity and the hydrolysis efficiency of cellulose are improved, and the STCE1 gene can be widely applied to industries such as spinning, cellulose hydrolysis, washing and the like.

The application discloses a method for preparing functional humus by using nano-iron composite catalyst to induce directional humification of straw and application thereof, and belongs to the technical field of agricultural solid waste resource utilization and humus synthesis. The method uses agricultural straw as raw material, improves the reaction activity through alkali pre-dissociation, and then uses ternary nano-iron composite catalyst composed of nano-Fe2O3, zero-valent nano-iron and nano-FeCl3 to perform directional hydrothermal humification, so as to induce directional condensation of straw lignocellulose hydrolysis products and enrich acid-containing oxygen and nitrogen functional groups. After the reaction, solid-liquid separation is performed and differential modification is performed, the solid phase is used to prepare nano-iron-based hydrothermal humic carbon for saline-alkali farmland improvement, and the liquid phase is used to prepare functional humic acid foliar fertilizer for crop growth promotion. The application shortens the reaction period to 4 hours, realizes directional regulation of functional groups and high-value utilization of all components of straw, and realizes synchronous improvement of soil fertility and yield increase of soybeans and corn through a matched regional application process.