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Inositol preparing method

A technology of myo-inositol and myo-inositol monophosphatase, applied in biochemical equipment and methods, lyases, transferases, etc., can solve problems such as high cost and low yield, and achieve low production cost, high yield and pollution. low effect

Active Publication Date: 2016-11-23
CHENGDU BOHAODA BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the hotspots of studying inositol production are chemical synthesis and microbial enzymatic hydrolysis. However, there are problems such as high cost and low yield to varying degrees.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0058] Experimental example 1 in vitro multi-enzyme catalyzed conversion of starch into inositol

[0059] Starch was converted to inositol by an in vitro multi-enzyme catalytic system ( figure 1 ). These key enzymes include: (1) Glucan phosphorylase (αGP, EC 2.4.1.1), which releases glucose-1-phosphate from starch; (2) Glucose phosphomutase (PGM, EC 5.4.2.2) , catalyzing glucose-1-phosphate to glucose-6-phosphate; (3) inositol-3-phosphate synthase (IPS, EC 5.5.1.4), which catalyzes glucose-6-phosphate to inositol-3-phosphate; ( 4) Inositol monophosphatase (IMP, EC 3.1.3.25), which dephosphorylates inositol-3-phosphate to inositol. Since the last two enzyme reactions are irreversible reactions, the enzyme-catalyzed system can obtain a high conversion rate.

[0060] In the present invention, the glucan phosphorylase is derived from Thermotoga maritima, and the number of the gene on KEGG is TM1168, and the phosphomutase of glucose is also derived from Thermotogamaritima, and ...

experiment example 2

[0064] Experimental example 2 in vitro multi-enzyme catalyzed conversion of starch into inositol

[0065] The preparation of glucan phosphorylase, glucose phosphomutase, inositol-3-phosphate synthase and inositol monophosphatase was the same as that in Experimental Example 1.

[0066] In a 0.75 ml reaction system containing 100 mM HEPES buffer (pH7.2), 10 mM inorganic phosphate, 5 mM divalent magnesium ion, 0.5 mM zinc ion, 0.05 U / mL dextran phosphorylase, 1U / mL glucose phosphomutase, 0.05U / mL inositol-3-phosphate synthase and 2U / mL inositol monophosphatase, 10g / L soluble starch, catalyzed reaction at 40℃, 40 reactions Hour.

[0067] After the reaction, the final concentration of inositol was 0.9g / L, and the conversion rate was 9%.

experiment example 3

[0068] Experimental example 3 in vitro multi-enzyme catalyzed conversion of starch into inositol

[0069] The preparation of glucan phosphorylase, glucose phosphomutase, inositol-3-phosphate synthase and inositol monophosphatase was the same as that in Experimental Example 1.

[0070] In a 0.75 ml reaction system containing 100 mM HEPES buffer (pH7.2), 10 mM inorganic phosphate, 5 mM divalent magnesium ion, 0.5 mM zinc ion, 0.05 U / mL dextran phosphorylase, 1U / mL glucose phosphomutase, 0.05U / mL inositol-3-phosphate synthase and 2U / mL inositol monophosphatase, 10g / L soluble starch, catalyzed reaction at 80℃, 40 reactions Hour.

[0071] After the reaction, the final concentration of inositol was 3.6g / L, and the conversion rate was 36%.

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Abstract

The invention discloses an inositol preparing method and belongs to the field of enzymatic preparation of inositol. According to the method, starch, cellulose or derivatives thereof are used as substrates and converted into inositol in a multi-enzyme reaction system through in-vitro multi-enzyme efficient catalysis. By conducting process optimization, adding enzymes capable of promoting starch or cellulose hydrolysis and utilizing the enzyme of the byproduct glucose, the multi-enzyme reaction system is established, and raw material conversion efficiency and inositol yield are improved remarkably. By the adoption of the method, raw material conversion rate is high, inositol yield is high, steps are simple, production cost is low, influence on the environment is small, and large-scale production of inositol can be achieved.

Description

technical field [0001] The invention relates to a preparation method of inositol, in particular to a method for converting starch or cellulose and their derivatives into inositol by multi-enzyme catalysis in vitro, and belongs to the field of enzymatic catalysis production of inositol. Background technique [0002] Inositol, also known as cyclohexanol, is one of the water-soluble vitamin B family. Inositol is an essential substance for the growth of humans, animals and microorganisms, and is widely used in medicine, food, feed and other industries. At present, the global demand is about 5,000 tons per year. Due to the current high price of inositol, the market prospect of inositol has not been fully developed. For example, the global feed production in 2013 was 960 million tons. If 0.2- 0.5% inositol, then the output of inositol required by the feed industry should reach 1.9-4.8 million tons per year. Under such circumstances, the current production in China and even in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/02
CPCC12N9/2437C12P7/02C12P2203/00C12Y204/01001C12Y204/0102C12Y204/01049C12Y301/03025C12Y504/02002C12Y505/01004
Inventor 张以恒游淳
Owner CHENGDU BOHAODA BIOLOGICAL TECH CO LTD
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