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Method of extracting DHA unsaturated fatty acid from dino flagellate fermentation liquor

A technology of unsaturated fatty acids and dinoflagellates, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of energy consumption, solvent consumption, pollution discharge, etc., and achieve quality assurance, low process cost, and process The effect of cost and environmental protection

Active Publication Date: 2009-11-25
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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Problems solved by technology

However, the above-mentioned extraction method basically uses a large amount of solvents for extraction, such as chloroform and n-hexane, which need to be concentrated and removed after extraction, resulting in a large amount of energy consumption and solvent consumption, and the formation of polluting emissions

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  • Method of extracting DHA unsaturated fatty acid from dino flagellate fermentation liquor
  • Method of extracting DHA unsaturated fatty acid from dino flagellate fermentation liquor

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Embodiment Construction

[0009] In order to specifically illustrate the process of the present invention, the following is an embodiment that can implement the present invention, but it is not a limitation to the present invention. Take 100L of dinoflagellate fermentation broth, add 20% hydrochloric acid while stirring, and adjust the pH to 5.5. Continue to add 3L of 0.3% non-residual monomer cationic polyacrylamide aqueous solution, fully stir for 30 minutes, stop stirring and let stand for 60 minutes. The material liquid is hit to a plate and frame filter for pressure filtration, and the filter cake is collected. Add 0.15Kg each of endoglucanase EC3.2.1.4, ex-glucanase EC3.2.1.91, and glucosidase EC2.1.21 to 50L of water, stir well and add the aforementioned filter cake, 20-40℃ Stir for 60 minutes while keeping warm. Obtain broken fermentation broth. The broken wall fermentation broth is fed into the Westfalia high-speed disc centrifugal separator of Germany, and the rotation speed is 10,000 rpm, and th...

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Abstract

The invention discloses a method of extracting DHA unsaturated fatty acid from dino flagellate fermentation liquor; the method comprises the main processes of: firstly adjusting pH value of dino flagellate fermentation liquor by using hydrochloric acid solution, taking non-residual monomer polyacrylamide as flocculant for flocculating; filtering by flocculating frame to obtain filter cake; breaking the filter cake by broken liquid prepared by adding water in compound cellulose hydrolase; after breaking, using centrifugal machine to realize three-phase separation of residue, aqueous phase and oil phase, repeating 2 to 5 times until no oil phase existing, combining the oil phase and washing, that is DHA crude oil; the crude oil entering into five class continuous molecular to distill, thereby obtaining unsaturated fatty acid having 40-50% of DHA. The invention uses high speed centrifuge with rotation rate being 8000 to 12000 rpm to separate to directly obtain crude oil, avoids the use of organic solvent; the refining of the crude oil utilizes five class molecular distillation, which can effectively achieve the effects of decolorization, deacidification and deodorization. The invention has low process cost and is environment-friendly.

Description

Technical field [0001] The invention relates to a method for extracting unsaturated fatty acids, in particular to a method for extracting DHA unsaturated fatty acids from the fermentation broth of Crypthecodinium cohnii. Background technique [0002] The chemical name of DHA is all-cis-4,7,10,13,16,19-docosahexaenoic acid. It is one of the main components of human brain and retina. DHA plays an important role in the neurodevelopment of infants. , Infant’s intellectual development index and nervous system development are positively correlated with DHA. DHA has the following physiological health care functions for the elderly: (1) Lowering blood lipids; (2) Inhibiting platelet aggregation and reducing the formation of thrombus; (3) Anti-atherosclerosis and preventing myocardial infarction; (4) Preventing senile dementia , Delay aging; (5) prevent cancer. Since the 1990s, DHA has become a nootropic health product popular all over the world. FOA (World Agriculture and Food Organizati...

Claims

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Application Information

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IPC IPC(8): C07C57/03C07C51/42C07C51/44
Inventor 詹光煌吴轶付志杰
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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