Separation of tropical candiyeast strain and production of xylitol

A Candida and xylitol technology, applied in the biological field, can solve the problems of low product production rate, unsuitable for industrial production, and low production efficiency

Active Publication Date: 2007-06-20
唐传生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The purpose of the present invention is to effectively solve the problem that the overall production efficiency is too low due to the low product concentration and low product formation rate in the xylitol fermentation process using hemicellulose, which is not suitable for industrial production.

Method used

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  • Separation of tropical candiyeast strain and production of xylitol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Seed medium composition: xylose 15g / L, glucose 15g / L, yeast extract 10g / L, KH 2 PO 4 , 1.5g / L, NH 4 h 2 PO 4 , 3g / L, MgSO 4 .7H 2 O, 0.1g / L, pH5-6, sterilized at 115C for 15 minutes. Xylose is sterilized separately from other ingredients, and the volume of a 250ml triangular bottle is 30ml. Take CCTCC: M205067 test tube slant culture to inoculate, and shake the flask at 30°C for 15 hours to activate the seeds. Take the activated seed culture solution, transfer it to a fresh seed medium at an inoculum size of 5% (v / v), continue to shake the flask for 15 hours, and obtain liquid seeds of about 60 g fresh cells / L.

Embodiment 2

[0072] Dry 40kg of bagasse, put it into a 500L enamel hydrolysis tank, add 300L of 2.5% sulfuric acid (w / v), directly feed steam, and heat to 120°C for 2.5h for hydrolysis. Release the hydrolyzate in the tank, and wash the residue once with water. Combine the solutions, neutralize the sulfuric acid with CaCO3, filter out the calcium sulfate precipitate, concentrate the solution under reduced pressure to a soluble solid content of 10%, pass through HPD500 macroporous adsorption resin column chromatography, and treat with activated carbon, and finally concentrate the solution to obtain usable It is a hydrolyzate syrup with a xylose content of about 300g / L for the production of xylitol by fermentation. The macroporous adsorption resin column chromatography detoxification method is introduced in the published article of the inventor (Acta Bioengineering, 2002, 18(6): 725-728).

Embodiment 3

[0074] 1000g of dried corncobs, crushed into particles with a diameter of about 1-5mm, add 10kg of water, boil for 30min, filter out the boiling liquid, and repeat the boiling once. Add 8L of 2.5% (w / v) sulfuric acid to the corncobs that have been filtered out of the boiled liquid, and hydrolyze at 120°C for 2.5h. The hydrolyzate was collected, and the residue was boiled once with water. Combine the two solutions, CaCO 3 Neutralize sulfuric acid, filter out calcium sulfate precipitate, concentrate the solution under reduced pressure to a soluble solid content of 10%, pass HPD500 macroporous adsorption resin column chromatography, and treat with activated carbon, and finally concentrate under reduced pressure until the xylose content reaches 300g / L . This hydrolyzate is directly used for fermentation to prepare xylitol.

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Abstract

Separation of tropical moniliasis strain and production of xylitol are disclosed. The process is carried out by amplification culturing to obtain seed liquid, preparing hemicellulose hydrolyte culture medium, circulating and reutilizing. It has excellent sugar-tolerance and mithridatism. It could be used above time times.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production method in which yeast strains isolated from nature are enlarged and then used to ferment hemicellulose hydrolyzate, and xylose in the hydrolyzate is converted into xylitol. Background technique [0002] Xylitol is a five-carbon sugar alcohol with high sweetness, good anti-caries performance, no insulin intervention in the metabolic process, and a large heat of solution that can make the mouth feel particularly cool, making it very useful in the food industry. important application value. [0003] At present, the industrialized production of xylitol mainly uses the process of reducing xylose by chemical method. The basic process is: first hydrolyze the hemicellulose part in the lignocellulosic material with dilute acid, and then extract the hemicellulose from the hemicellulose hydrolyzate rich in xylose Purify xylose, and finally catalyze the hydrogenation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/16C12N15/31C12P7/18C12P19/02C12R1/74
Inventor 张厚瑞曾健智蔡爱华何成新
Owner 唐传生物科技(厦门)有限公司
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