Method for producing ethanol by high-efficiency simultaneous saccharification and cofermentation

A simultaneous saccharification and co-fermentation technology, which is applied in the fields of fermentation engineering, biomass energy, and biochemical industry, can solve the problems of insufficient industrialization potential, low comprehensive utilization rate, and poor separation effect, so as to improve the process of adsorption and desorption, The effect of reducing equipment investment and reducing enzyme consumption

Inactive Publication Date: 2011-11-23
GUANGZHOU YOURUI BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of high-efficiency synchronous saccharification and co-fermentation of bagasse to solve the bottlenecks such as poor separation effect, low conversion rate, low comprehensive utilization rate and insufficient industrialization potential in the current ethanol production process using lignocellulose as raw material. Methods of producing ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for producing ethanol by bagasse high-efficiency synchronous saccharification and co-fermentation described in this embodiment mainly includes the following steps:

[0040] (1) Pretreatment: Weigh 1000g of dry bagasse, soak it in dilute sulfuric acid, and perform steam explosion treatment after filtration; add hot water to wash the soluble hydrolyzate after steam explosion bagasse, and filter to obtain washing liquid and filter residue; add lower alcohol-alkaline water to the filter residue The mixed system was stirred and extracted at high temperature in an autoclave, filtered and separated, and washed with water to obtain an extraction residue and a washing solution; the recovery rate of cellulose from the extraction residue (calculated as glucose) was 86%;

[0041] (2) Concentrated detoxification: Concentrate about 30L of the washing liquid in step (1) in vacuum to about 5L, add 1% (W / W) Ca(OH) 2 Carry out the removal treatment of toxic inhibitors, filter,...

Embodiment 2

[0047] The method for producing ethanol by bagasse high-efficiency synchronous saccharification and co-fermentation described in this embodiment mainly includes the following steps:

[0048] (1) pretreatment: with embodiment 1;

[0049] (2) Concentrated detoxification: Concentrate about 30L of the washing liquid in step (1) in vacuum to about 3L, add 5% (W / W) Ca(OH) 2 Carry out the removal treatment of toxic inhibitors, filter, adjust the pH of the filtrate to neutrality with 2% (W / W) dilute sulfuric acid, and separate the solid and liquid to obtain the detoxified concentrated water washing solution. The xylose concentration is determined to be 60.1g / L; The prime recovery rate (in terms of xylose) is 62%;

[0050] (3) Preparation of yeast seed liquid: Activate the slant of Saccharomyces cerevisiae strain and insert it into the seed medium: glucose 10g / L, yeast extract 5.0g / L, NH 4 Cl 1.0g / L, MgSO 4 ·7H 2 O0.5g / L, KH 2 PO 4 0.5g / L; activate the Pachysolen tannophilus sla...

Embodiment 3

[0054] The method for producing ethanol by bagasse high-efficiency synchronous saccharification and co-fermentation described in this embodiment mainly includes the following steps:

[0055] (1) pretreatment: with embodiment 1;

[0056] (2) Concentrated detoxification: Concentrate about 30L of the washing liquid in step (1) in vacuum to about 4L, add 2% (W / W) Ca(OH) 2 Carry out the removal treatment of toxic inhibitors, filter, adjust the pH of the filtrate to neutrality with 2% (W / W) dilute sulfuric acid, and separate the solid and liquid to obtain the detoxified concentrated water washing solution. The xylose concentration is determined to be 36.1g / L; The prime recovery rate (in terms of xylose) is 62%;

[0057] (3) Preparation of yeast seed liquid: Activate the slant of Saccharomyces cerevisiae strain and insert it into the seed medium: glucose 15g / L, yeast extract 3.0g / L, NH 4 Cl 1.5g / L, MgSO 4 ·7H 2 O0.3g / L, KH 2 PO 4 0.7g / L; activate the slant of Candida Shehatae (C...

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Abstract

The invention discloses a method for producing ethanol by high-efficiency simultaneous saccharification and cofermentation, which comprises the following steps: pretreating; concentrating and detoxifying; preparing yeast seed solution; performing simultaneous saccharification and cofermentation; and distilling/rectifying. When the method provided by the invention is used, the inhibition action of a monosaccharide component formed by hydrolysis of cellulose on cellulose is relieved, the enzymolysis saccharification efficiency and fermentation yield are improved, the recovery rate of hemicelluloses in bagasse is about 60 percent, the cellulose recovery rate is about 86 percent, and the total ethanol yield of dry bagasse can reach about 20 percent.

Description

technical field [0001] The invention belongs to the fields of biochemical engineering, fermentation engineering and biomass energy, and in particular relates to a method for producing ethanol through efficient synchronous saccharification and co-fermentation of bagasse. technical background [0002] Lignocellulose is the most abundant and cheap renewable resource on earth, with an annual production of up to 2×10 11 t. The biotransformation of lignocellulose has important practical significance for solving energy problems and alleviating environmental pollution. The annual available lignocellulose in my country is about 700 million tons, mainly from agricultural and forestry waste, industrial waste and urban waste. Bagasse is the main fibrous residue by-product of the sugarcane sugar industry. The yield of dry bagasse is generally 11.5% to 13%. The total annual output of sugarcane in my country is more than 70 million tons, and bagasse will reach more than 7 million tons. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/10C12P19/14C12R1/865C12R1/84C12R1/645C12R1/72
CPCY02E50/16Y02E50/10
Inventor 刘立国杨尉林香瑶刘柏楠
Owner GUANGZHOU YOURUI BIOSCI
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