7results about How to "Increase hydrolysis rate" patented technology

The invention relates to the technical field of isoflavone biotransformation, in particular to a soybean isoflavone aglycone preparation method based on enzymolysis. Comprising the following steps: inoculating Kluyveromyces marxianus into a basic culture medium for shake cultivation; after culturing, centrifuging, washing and resuspending to obtain a bacterial suspension as a leavening agent, and inoculating the bacterial suspension to a bean dreg composite culture medium for shaking table fermentation for 48-96 hours; centrifuging the fermentation liquor at 0-4 DEG C, and collecting supernate to obtain crude beta-glucosidase; the crude enzyme liquid is sequentially subjected to ammonium sulfate salting-out, ion exchange chromatography and twice sephadex chromatography, and purified beta-glucosidase liquid is obtained; and carrying out enzymatic reaction on soy isoflavone by using the purified beta-glucosidase liquid to obtain the soy isoflavone aglycone. The method has the advantages that the bean dreg culture medium is utilized for fermentation to produce beta-glucosidase, and the combined soy isoflavone is subjected to enzymolysis, so that the utilization of the byproduct bean dregs is improved, the biotransformation of isoflavone is realized, and the obtained product has better activity.

The invention discloses a recycling method of polyethylene glycol terephthalate (PET). According to the method, organic acid is used as a catalyst for hydrolysis of PET, the organic acid is selected from one or more of adipic acid, lactic acid and glycollic acid, and then hydrolysis products are directly polymerized with additional monomers and polymerization catalysts to prepare the biodegradable copolyester without any separation treatment step. The prepared copolyester has biodegradability, so that the non-degradable PET is converted into the biodegradable copolyester by the recycling method, and the recycling method has important significance for relieving the problem of environmental pollution.

The invention discloses a method for simulating small intestines to digest and absorb starch-based food, and belongs to the technical field of food digestion detection. According to the method, the glucose adsorbent is added into the in-vitro small intestine digestion simulation system, so that the influence of glucose generated in the digestion process on the digestion system is reduced, and the digestion effect of the starch-based food in the small intestine is more truly reduced; the pitch-based spherical activated carbon is added in the simulated digestion process, so that glucose can be adsorbed, the enzyme reaction process is effectively improved, the hydrolysis rate of starch is increased, and the starch is more in line with the digestion and absorption mechanism of a human body on the starch.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

The application provides a titanium dioxide type low-temperature hydrogenolysis catalyst and a preparation method and application thereof. The catalyst comprises a titanium dioxide carrier and an active component loaded on the titanium dioxide carrier, and the active component comprises scandium oxide and tungsten oxide. The preparation method of the catalyst comprises the following steps: dipping the titanium dioxide carrier in a mixed solution of scandium salt and tungsten salt for a period of time, so that the scandium salt and the tungsten salt are loaded on the titanium dioxide carrier; and then drying and calcining the titanium dioxide carrier loaded with the scandium salt and the tungsten salt, to obtain the titanium dioxide type low-temperature hydrogenolysis catalyst. The catalyst has good sulfur dioxide hydrogenation activity and organic sulfur hydrolysis activity, and can be applied to a process for treating sulfur recovery tail gas at a low temperature of 210-220 DEG C.

The invention discloses isoamylase and a gene thereof, an engineering bacterium containing the gene and application of the engineering bacterium. The invention provides an isoamylase gene, the nucleotide sequence of which is as shown in SEQ ID NO.1, the full length of the gene is 2130 bp, the G + C content is 69.11%, 709 amino acids are encoded, and the amino acid sequence is as shown in SEQ ID NO.2; the isoamylase provided by the invention has wide pH range adaptability, has higher enzyme activity when the reaction is carried out at the pH of 6.0-8.0, and has the highest enzyme activity when the reaction is carried out under the conditions that the temperature is 40 DEG C and the pH is 7.0; the isoamylase provided by the invention has a hydrolysis effect on alpha-1, 4 and alpha-1, 6 glucosidic bonds, the specific activity of hydrolyzed corn starch is 250.46 U / mg, and when the isoamylase is combined with maltogenic amylase BMAL for use, the utilization efficiency of the maltogenic amylase BMAL on a branched chain substrate can be improved.

This invention discloses a method for preparing fatty acids from acidified oil, belonging to the field of biochemical engineering. The method includes preparing an immobilized lipase and enzymatic hydrolysis; the preparation of the immobilized lipase includes preparing a carrier and immobilization; the preparation of the carrier involves placing magnetic Fe... 3 O 4 The granules, anhydrous ethanol, deionized water, and ammonia were mixed, stirred at room temperature, and ultrasonically vibrated. Then, they were mixed with tetraethyl orthosilicate, stirred at room temperature, collected by magnetic decantation, and washed to obtain Fe. 3 O 4 @SiO 2­ Particles; Fe 3 O 4 @SiO 2­ Granules, hexadecyltrimethylammonium bromide, and deionized water are mixed and stirred. Calcium nitrate tetrahydrate is added and stirred. The pH value is adjusted to 9.5-10. Diammonium hydrogen phosphate aqueous solution is added dropwise and the pH value is stabilized at 9.5-10. The mixture is stirred and collected by magnetic decantation. The method of this invention can simultaneously achieve high hydrolysis rate and good reusability, and the hydrolysis rate is less affected by the acid value of acidified oil.