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Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers

A technology of galactomannan and galactomannan oligosaccharides, which is applied in the field of galactomannan-rich soluble dietary fiber and its preparation, can solve the problems of soluble dietary fiber that have not yet been seen, and achieve great application value and transformation High efficiency and good stability

Active Publication Date: 2017-02-15
CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no literature and patent reports about the preparation of soluble dietary fiber rich in galactomannan oligosaccharides by using guar gum

Method used

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  • Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers
  • Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers
  • Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers

Examples

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Embodiment 1

[0041]A soluble dietary fiber rich in galactomannan, with a weight average molecular weight of 24800 Da, wherein the mass percentage of galactomannan oligosaccharide is 20%-30%, and the polymerization degree of galactomannan oligosaccharide is 2-6.

[0042] The present invention also provides a preparation method of galactomannan-rich soluble dietary fiber, comprising the following steps:

[0043] S1: Preparation of recombinant bacteria containing β-mannanase, wherein the coding gene of β-mannanase is derived from Rhizomucor miehei CAU432;

[0044] S2: Microbial fermentation: β-mannanase is prepared by fermentation of recombinant bacteria;

[0045] S3: using β-mannanase to hydrolyze the guar gum solution;

[0046] S4: Filtrating, decolorizing, ion-exchanging and concentrating the enzymolysis solution in step S3 to obtain soluble dietary fiber rich in galactomannan.

[0047] As a preferred implementation of this example, in step S1, the recombinant bacterium is Pichia pastori...

Embodiment 2

[0053] 1. Acquisition of recombinant bacteria

[0054] S1: Using Rhizomucor miehei CAU432cDNA as template, using ManF(5′-CCATG TACGTA GCTTCTTCGTTTGTCCAGACAAG-3′) and ManR (5′-CCG CCTAGG TCACTTCTTGGCCATGGCATC-3′) primer pair, according to the method provided by Polit et al. (Katrolia et al. Journal of Agricultural and Food Chemistry, 2013, 61:394-401) for PCR amplification to obtain PCR amplification products.

[0055] S2: Perform double enzyme digestion on the PCR amplification product obtained in S1 with restriction endonucleases SnaBI and AvrII, and recover the digested DNA fragments; perform double enzyme digestion on the pPIC9K vector with restriction endonucleases SnaBI and AvrII, Reclaim the backbone vector after the digestion; the DNA fragment is connected with the backbone vector to obtain the recombinant vector A (i.e., between the SnaBI and AvrII restriction sites of the pPIC9K vector, the gene encoding Rhizomucor miehei source β-mannanase is inserted. DNA fragme...

Embodiment 3

[0079] Example 3, Experimental results of the hydrolysis of guar gum by β-mannanase at different hydrolysis times

[0080] Weigh 5g of guar gum and dissolve it in 100mL of distilled water, add β-mannanase according to the ratio of guar gum to 1000U / g, and place them at 50°C to hydrolyze 1, 2, 4, 8, 12, After 24 hours of enzymolysis, inactivate in a boiling water bath for 10 minutes to obtain an enzymolysis solution, which was centrifuged at 10,000 rpm for 10 minutes, and the supernatant was collected to obtain a crude sugar solution. The viscosity of the crude sugar solution was measured with a DV-1 rotational viscometer at 25°C, and the reducing sugar content in the crude sugar solution was determined by the 3,5-dinitrosalicylic acid method and the yield of reducing sugar was calculated.

[0081] Table 1 shows the viscosity of crude sugar solution and the yield of reducing sugar obtained by hydrolyzing guar gum at different hydrolysis times.

[0082] Table 1 Viscosity of cru...

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Abstract

The invention discloses soluble dietary fibers rich in galactomannan and a preparation method of the soluble dietary fibers. The mass percentage of galactomannan is 20-30%. Beta-mannase provided by the invention is better in stability and higher in activity than enzyme, and has great application value in the trade of foods, feeds and the like. According to the preparation method provided by the invention, the hydrolysis rate and the galactomannan conversion rate are high, products are easy to separate, and the weight-average molecular weight of finished products is about 24800Da; and two kinds of products of soluble dietary fiber syrup and powdered sugar, containing the galactomannan, can be produced by the preparation method provided by the invention, besides, the yield of products is high, the yield of the soluble dietary fiber syrup is 74.2%, and the yield of the powdered sugar is 72.8%.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a soluble dietary fiber rich in galactomannan and a preparation method thereof. Background technique [0002] Dietary fiber is a polysaccharide that can neither be digested and absorbed by the gastrointestinal tract nor produce energy. Physiological functions of dietary fiber: this kind of substance can stimulate intestinal peristalsis, facilitate excrement of feces, prevent constipation, rectal cancer, hemorrhoids and varicose veins of lower extremities; prevent the occurrence of cardiovascular diseases such as atherosclerosis and coronary heart disease; prevent The formation of gallstones; produce a sense of satiety, which is beneficial for obese patients to eat, and can be used as a weight-loss food; improve sugar tolerance, regulate blood sugar levels of diabetics, and can be used as food for diabetics; improve intestinal flora, prevent intestinal cancer, Appendici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21A23L33/26C12N9/42C12P19/14C12P19/04C12R1/84
CPCC12N9/2494C12P19/04C12P19/14C12Y302/01078
Inventor 江正强刘燕静李延啸袁江宏闫巧娟张伟
Owner CHINA AGRI UNIV
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