Method for preparing fatty acid by using lipase hydrolyzation of oil and fat

A technology for hydrolyzing oil and lipase, applied in the biological field, can solve the problems of increasing the hydrolysis rate of emulsifiers and speeding up the reaction process, and achieving the effects of less enzyme consumption, lower production costs, and increased collision opportunities

Active Publication Date: 2008-10-29
领先生物农业股份有限公司
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only a few research articles have shown that the use of free or immobilized lipase (esterase) can be used for oil hydrolysis (Gu Wenling. Research progress in lipase oil hydrolysis technology. Western Grain and Oil, 1998, 23 (6): 31-35 Cao Guomin. Silk-immobilized lipase catalyzes the hydrolysis of oliv

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing fatty acid by using lipase hydrolyzation of oil and fat

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] Example 1

[0058] Add 2 grams of commercially available Koufu brand soybean oil to a 50ml conical flask with a ground mouth and stopper, and then add 1.2ml of enzyme solution prepared with pH6-pH7 phosphate buffer solution (weight ratio of oil to water is 1:0.6, enzyme powder is The lipase powder produced by Wuxi Enzyme Preparation Factory, the enzyme activity is 80U / ml), the pH of the aqueous phase is adjusted to 6.0 with 2N NaOH solution, and the shaking reaction is carried out at 40°C and 130 rpm for 36 hours. The obtained fat hydrolysate is determined by the acidity analysis method to have an acid value of 125 mgKOH / g oil, and the hydrolysis rate is 62.5% calculated according to the above method.

[0059] The obtained hydrolyzate is acidified with 2N sulfuric acid to make the pH of the water phase reach 4.5, and then heated to 68° C., stand for layering, and release the water phase (sweet water containing glycerin) to obtain crude fatty acids with a small amount of wat...

Example Embodiment

[0062] Example 2

[0063] Add 10 grams of palm oil sold by Qinhuangdao Jinhai Cereals and Oils Industry Co., Ltd. under the trade name palm oil to a 250ml conical flask with a stopper, and then add 0.1g NOVOZYM435 (enzyme activity 10000 PLU / g, PLU / g refers to every 1g enzyme The generated propyl laurate unit), adjust the weight ratio of oil to water to 1:0.2 with a pH 6-7 phosphate buffer solution, add 1.4 g of sodium stearate, and adjust the pH of the water phase to 5.7 with 2N NaOH solution. 45°C, 130 rpm shaking reaction for 30 hours. The acid value of the obtained hydrolyzate was determined by acidity analysis method to be 162 mgKOH / g oil, and the hydrolysis rate calculated according to the above method was 81%.

[0064] The obtained hydrolysate is acidified with 3N sulfuric acid to make the pH of the water phase reach 4.5, and then heated to 70°C, stand still for layering, and release the water phase (sweet water containing glycerin) to obtain crude fatty acids with a small a...

Example Embodiment

[0066] Example 3

[0067] Add 1000 g of soybean acidified oil sold by Qinhuangdao Jinhai Cereals and Oils Industry Co., Ltd. into a 2000ml four-neck reactor, and add 600ml of enzyme solution prepared with pH6-pH7 phosphate buffer solution (weight ratio of oil to water is 1:0.6, enzyme powder is Wuxi Lipase powder produced by the enzyme preparation factory, enzyme activity 80U / ml), sodium stearate 140g, 2N NaOH solution is used to control the pH of the water phase to 6.5-7.0, and the reaction is stirred at 40°C and 100 rpm for 36 hours. The obtained fat hydrolysate is determined to have an acid value of 143 mgKOH / g oil by the acidity analysis method, and the hydrolysis rate is 71.5% calculated according to the above method.

[0068] The obtained hydrolyzate is acidified with 3N sulfuric acid to make the pH of the water phase reach 4.5, and then heated to 65° C., stand still for stratification, and release the water phase (sweet water containing glycerin) to obtain crude fatty acids...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Acid valueaaaaaaaaaa
Acid valueaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for producing fatty acid by hydrolyzing animal and vegetable oils or waste animal and vegetable oils using lipase. The method can achieve a hydrolysis rate above 90% by carrying out hydrolysis reaction using lipase in the presence of emulsifying agent. The enzymatic method of the invention is carried out under a mild condition to overcome the disadvantages of prior alkaline saponification, high-temperature high-pressure continuous cracking and medium-pressure cracking reaction, and has the advantages of less energy consumption, low equipment cost, no contaminant discharge, and high applicability for raw materials. Accordingly, the enzymatic method of the invention has wide application prospect.

Description

【Technical field】 [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for producing fatty acids using lipase. 【Background technique】 [0002] Fatty acid is an important chemical, daily chemical, light industry, pharmaceutical, and food raw material, especially in daily chemical, surfactant synthesis, and food. In the past, fatty acids were mainly produced from animal and vegetable oils by hydrolysis through physical and chemical methods. The reaction required heating, pressurization, or even high temperature and high pressure (for example, the continuous cracking method required 250°C, 5.5MPa), or under strong alkaline conditions ( Alkaline Saponification). Physicochemical lipolysis not only consumes a lot of energy, but also has high requirements for equipment due to severe reaction conditions. In addition, severe reaction conditions will increase side reactions and by-products, which will affect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/64C12P7/20
Inventor 徐志文孟永宏周志奇王东李月娟
Owner 领先生物农业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap