124 results about "Immobilized lipase" patented technology

The invention relates to the technical field of food, in particular to diglyceride for reducing triglyceride, cholesterol and uric acid as well as a biological enzyme preparation method and application of the diglyceride. The preparation method comprises the following steps: (1) adding water and glycerol into vegetable oil, and then adding immobilized lipase to react to obtain a reactant; (2) centrifuging reactants, collecting a light-phase product, carrying out molecular distillation, and collecting a heavy-phase product to obtain diglyceride; the immobilized lipase comprises lipase and a carrier, the carrier comprises loofah sponge, soybean meal and bentonite, and the mass ratio of the loofah sponge to the soybean meal to the bentonite is 1: (2-4): (3-6). The method has the advantages that the effects of reducing triglyceride, total cholesterol and uric acid are relatively good, and the yield and the purity are relatively high.

The invention relates to the field of immobilized enzymes, in particular to immobilized lipase with graft-modified amino resin as a carrier and a preparation method thereof.The method comprises the steps that firstly, the amino resin is modified through the oxidative auto-polymerization characteristic of tannic acid, and first modified amino resin is obtained; and modifying the first modified amino resin by using fatty amine as a hydrophobic modifier to obtain second modified amino resin. Then lipase is fixed on the second modified amino resin through physical adsorption, the lipase is subjected to interfacial activation on an amphiphilic interface on the second modified amino resin, and a part of lipase is covalently bound with quinonyl in an activated state, so that the immobilized lipase with high activity and stability is obtained. The method is easy to operate, green and non-toxic, the prepared immobilized lipase is good in enzyme activity and stability, and the method can be used for preparing the immobilized lipase on a large scale, can be applied to the field of food and has industrial application potential.

The invention discloses immobilized lipase based on bifunctional silicon dioxide as well as a preparation method and application of the immobilized lipase. Hydrophobic modification and covalent modification are performed on the surface of mesoporous silicon dioxide to obtain a bifunctional silicon dioxide carrier; the hydrophobic modification is any one of methylation modification, octylation modification, octadecyl alkylation modification, esterification modification and bromopropylation modification. The dual-functionalized silicon dioxide carrier is used for immobilization of lipase, and the prepared immobilized lipase can be used for production of biodiesel. The immobilized lipase provided by the invention is mild in enzyme reaction condition, good in biocompatibility, relatively high in enzyme activity, and excellent in stability and repeatability. In addition, by changing the ratio of the covalent modifier to the hydrophobic modifier, the catalytic activity and stability of the immobilized enzyme and the biodiesel yield are remarkably improved.

The invention discloses a method for synthesizing ceramide NP through two-step enzyme catalysis, which comprises the following steps of: firstly, adding lipase into vegetable oil to catalyze and combine fatty acid to decompose into free fatty acid, and centrifuging to remove the lipase; a small amount of phytosphingosine is added into the hydrolyzed vegetable oil, the raw material vegetable oil is used as a solvent or a squalane system solvent for incubation, and then immobilized lipase is added for catalytic synthesis of ceramide. The invention establishes a two-enzyme method for efficiently synthesizing the ceramide NP compound, and the process is natural, environment-friendly and non-toxic. The reaction conditions are optimized through response surface analysis, meanwhile, the immobilized lipase shows excellent cycle performance, and the method is easy to operate, environmentally friendly, green and economical and has wide application prospects in the fields of cosmetics, health care products, biological medicine and the like.

The invention relates to the technical field of shoe materials, in particular to a bio-based EVA-containing composite shoe material and a preparation method thereof, and the bio-based EVA-containing composite shoe material is prepared from the following raw materials in parts by weight: a bio-based EVA polymer, bio-based TPU, a cross-linking agent, a compatilizer, a nucleating agent, filler, a foaming agent, a plasticizer, a flexible component, a biodegradation accelerant, a non-polyolefin elastomer and maleic anhydride grafted polyethylene wax. Wherein the compatilizer can be ethylene-vinyl alcohol and the like; the nucleating agent can be nanoscale talcum powder and the like; the filler can be ethyl cellulose and the like; the foaming agent is an azodicarbonamide foaming agent; the biodegradation accelerant is prepared from immobilized lipase, a tannic acid-Fe < 3 + > complex and surface carboxylated TiO2. The preparation method comprises two steps of mixing granulation and supercritical foaming. The composite shoe material provided by the invention has good biodegradability, can reduce environmental pollution, and maintains excellent physical and mechanical properties at the same time.

The invention discloses a high-activity lipase mutant and application thereof in functional grease synthesis, and belongs to the technical field of enzyme engineering and the technical field of enzyme immobilization. According to the invention, the lipase from Burkholderia B. cepacian OCRI-Lip100 screened in the early stage is truncated and mutated, and the soluble expression efficiency in Escherichia coli is improved by adding a solubilizing tag at the C end, co-expressing with folding enzyme and the like. According to the lipase mutant prepared by immobilization of the DMONs nano material, the half-life period of the obtained immobilized lipase at 60 DEG C is prolonged, the catalytic activity relative to free enzyme is improved, and the lipase mutant has good industrial application potential.

The invention belongs to the technical field of lipase immobilization, and particularly relates to immobilized lipase and a preparation method thereof. The preparation method comprises the following two steps: (I) preparation of activated amino type silica gel resin: mixing amino type silica gel resin, a nonionic surfactant 1308 and deionized water according to a mass ratio of (1000-1100): (2000-2200): (5-8), stirring at 1-5 DEG C for 10-15 minutes, adding cyanuric chloride, dripping a 30-35 wt% sodium hydroxide solution, reacting, and centrifuging; (II) preparation of immobilized lipase: mixing the activated resin, a surfactant, deionized water and a lipase solution, dropwise adding an alkaline solution at 40-45 DEG C under the condition that the pH is 5.0-5.5, and centrifuging, washing and drying after reaction. The obtained immobilized lipase is firm in covalent binding, the enzyme activity is greater than or equal to 50% after the immobilized lipase is repeatedly used for 6 times, the acid-base tolerance and the storage stability are excellent, and the immobilized lipase is suitable for industrial catalysis in multiple industries.

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The application discloses a hot air pretreatment method for extracting oat oil and belongs to the technical field of oil extraction. The application solves the technical problems of low extraction efficiency, easy oxidation of oil and large solvent consumption existing in the prior art. The method comprises the following steps: a. oat bran is dried by hot air at 130-150 DEG C for 15-20 min; b. the oat bran is placed in a negative pressure environment, and ethanol vapor flash treatment is performed for 5-15 min; c. the oat bran is mixed with a mixed solvent of petroleum ether and ethanol, immobilized lipase and natural antioxidant are added, and extraction is performed for 8-12 h, and then filtration separation is performed; d. the first residue is mixed with the mixed solvent, extraction is performed for 4-6 h, and then filtration separation is performed; e. the first supernatant and the second supernatant are combined, the solvent is recovered by reduced pressure distillation, and oat crude oil is obtained; and f. the immobilized lipase is recovered. The method is mainly used for efficiently extracting high-quality oat oil from oat bran.

The invention relates to the technical field of pure biodiesel preparation, and particularly discloses a method for continuously preparing pure biodiesel from kitchen waste oil based on immobilized enzyme. The invention discloses a method for continuously preparing pure biodiesel from kitchen waste oil based on immobilized enzyme. The method comprises the following steps: filtering, dehydrating and degumming the kitchen waste oil to obtain pretreated kitchen waste oil; mixing the pretreated kitchen waste oil with an acyl acceptor to obtain a reaction substrate; and feeding the reaction substrate into an enzyme reactor filled with the immobilized lipase catalyst, carrying out transesterification, collecting reaction effluent, and carrying out separation and purification to obtain pure biodiesel. According to the continuous production technology, separation of the catalyst and the reaction substrate is achieved, the catalyst can be recycled, the immobilized lipase catalyst prepared through the covalent binding method has high stability and activity, and the yield of pure biodiesel can still reach 84% or above after continuous operation exceeds 200 hours.

The application discloses a preparation method of medium and long chain fatty acid triglyceride, and belongs to the technical field of oil deep processing. Firstly, plant oil and medium chain fatty acid triglyceride are used as substrates, a low eutectic solvent is used as a reaction medium, and an ester exchange reaction is carried out under the catalysis of immobilized lipase; after the reaction is completed, centrifugal treatment is carried out, and the low eutectic solvent, the immobilized lipase and the reaction product are recovered respectively; the recovered low eutectic solvent and the immobilized lipase are used for the next batch of reaction, and the recovered upper oil phase is the reaction product. The application uses immobilized lipase to catalyze ester exchange to prepare medium and long chain fatty acid triglyceride in a low eutectic system, the substrate conversion rate and the product generation amount are high, the product separation is easy, further separation and purification are not needed, the low eutectic solvent is green, environment-friendly and reusable, and the method has good ecological, economic benefits and industrialization application prospects.

The invention belongs to the technical field of fat synthesis, and particularly discloses a method for synthesizing 1, 3-dilinoleic acid-2-palmitic acid triglyceride, which comprises the following steps: S1, dissolving palm stearate in a solvent, and purifying to obtain PPP; s2, mixing the PPP purified in the step S1 with LA, dissolving, and adding immobilized lipase ANL-MARE to carry out primary reaction; after the primary reaction is completed, carrying out acid removal; and then adding LA again to carry out secondary reaction, filtering to remove enzyme after the reaction is completed, and separating and purifying to obtain the LPL structure lipid. According to the method, PPP-rich palm stearin and linoleic acid are used as raw materials, and immobilized lipase is utilized to catalyze two-step acidolysis reaction in a solvent-free system to synthesize the structured triglyceride rich in LPL. Compared with a common one-step acidolysis method, the method has the advantages that the relative content of LPL is increased by 10.27%, the relative content of sn-2PA is kept unchanged, the usage amount of enzyme is effectively reduced, and the LPL efficiently synthesized through enzymatic catalysis of the method has the potential of being used for production of human milk fat substitutes.

This invention relates to the technical field of imiquimod synthesis, specifically to catalysts, methods, and applications for imiquimod synthesis. The invention employs a lipase-immobilized lipase complex carrier to catalyze a transesterification reaction, using vinyl isobutyrate as the acyl donor and tert-butanol as the solvent. The hydroxyl group of 3-(4-amino-1H-imidazo[4,5-C]quinoline-1-yl)-2-methylpropane-1-ol is converted to an isobutyl side chain. Following enzymatic catalysis and pH adjustment, hydrolysis is performed to release the isobutyl group, further generating imiquimod and isobutyric acid. The lipase selectively catalyzes transesterification, avoiding side reactions, and the ester bond is rapidly hydrolyzed to the target product under acidic conditions.

The invention provides a preparation method of a mesoporous MOFs (Metal-Organic Frameworks) material with water-acid dual stability and application of the mesoporous MOFs material in high-value conversion of grease. The preparation method comprises the following steps: firstly, preparing a mesoporous MOFs material of which the average pore size is greater than 10nm by a lauryl sodium sulfate mediated soft template method, and then dissociating an outer layer by using sodium citrate to increase the outer specific surface area of the outer layer, so as to obtain the mesoporous MOFs material (Meso-MIL-88A) with water-acid dual stability. The adsorption isotherm of the Meso-MIL-88A accords with a Langmuir model, the adsorption of macromolecular lipase belongs to single-molecule adsorption, and the defect that the specific enzyme activity is reduced due to enzyme accumulation caused by the fact that a macroporous material serves as an enzyme immobilization carrier is fundamentally avoided. The Meso-MIL-88A can be used for efficiently immobilizing lipase and synthesizing structural phospholipids, and has a good application prospect in the field of lipase-mediated grease high-valued treatment.

The invention discloses a method for catalytically synthesizing a dabigatran intermediate based on immobilized lipase. The method comprises the following steps: firstly, immobilizing optimized CALB lipase with an amino acid sequence as shown in SEQ ID NO: 2 by taking a dimethyl sulfoxide-resistant carrier comprising silanization modified mesoporous SiO2, polyvinylidene fluoride, polymethyl methacrylate and the like as carriers to obtain immobilized lipase, and then, carrying out a reaction on a compound II serving as a substrate in a DMSO organic phase, so as to obtain the immobilized lipase. The immobilized lipase is utilized to catalyze a compound II and a compound III to perform an addition reaction, and a dabigatran key intermediate compound I is efficiently and highly selectively generated; the immobilized carrier adopted by the invention can effectively tolerate a DMSO solvent, is firm in enzyme binding, can be repeatedly used for more than 10 times, has good activity retention, solves the problem that a biocatalyst is easy to inactivate and difficult to recover in a strong polar solvent, has the advantages of high conversion rate, good stereoselectivity, low production cost and environmental protection, and has a good industrial application prospect.

The invention relates to the technical field of biological catalysis, in particular to cyclodextrin-metal organic framework immobilized lipase, a preparation method and application of the cyclodextrin-metal organic framework immobilized lipase in grease catalysis. The cyclodextrin-metal organic framework immobilized lipase provided by the invention is simple in preparation process and controllable in cost, and has the advantages of high catalytic efficiency, good stability, strong reusability and the like; when the catalyst is applied to a grease catalytic reaction, the reaction condition is mild, the product is easy to separate, and the catalyst can be widely applied to the industrial fields of food processing, biodiesel preparation, fine chemical engineering and the like, and has remarkable economic value and social benefit.

The invention discloses a method for continuously catalyzing grease deacidification, which comprises the following steps: mixing a deep eutectic solvent, crude oil and immobilized lipase, homogenizing into an emulsion, continuously supplementing the crude oil, and carrying out esterification deacidification reaction, the immobilized lipase is obtained by carrying out immobilization by taking silane modified silicon dioxide as a carrier. According to the method, grease deacidification can be continuously catalyzed, the cost of enzymatic deacidification is reduced, the grease yield is increased, better economical efficiency and environmental friendliness are achieved, the industrial application potential is high, and after crude oil is deacidified through the method, the acid value is low, the deacidification rate is high, the neutral oil loss is small, and the oil product stability is good.

The invention relates to the technical field of organic synthesis and biochemical engineering, and discloses a preparation method of 3-o-ethyl ascorbic acid, which comprises the following steps: (1) carrying out pre-acetylation reaction on ascorbic acid and vinyl acetate under the catalysis of lipase to obtain 6-acetyl ascorbic acid; (2) carrying out ethylation reaction on the product in the step (1) and diethyl carbonate by taking ionic liquid as a catalyst; (3) purifying the product by combining supercritical COextraction with membrane filtration; according to the preparation method of the 3-o-ethyl ascorbic acid provided by the invention, the purity and the yield of the product are remarkably improved through a synergistic process of lipase pre-acetylation, ionic liquid catalytic ethylation and supercritical COextraction combined with membrane filtration and purification; 6-hydroxyl of ascorbic acid can be selectively protected. The step utilizes the specific catalysis function of the enzyme to avoid the non-selective reaction of other hydroxyl groups.

The invention relates to magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase based on amino acid ionic liquid modification as well as a preparation method and application of the magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase. The immobilized enzyme is prepared by taking Fe3O4 as a magnetic core, coating the magnetic core with dopamine, polymerizing to form a COF shell layer, modifying the surface of a carrier with amino acid ionic liquid (HisIL), and covalently immobilizing lipase (such as porcine pancreatic lipase), and the prepared immobilized enzyme is applied to catalytic synthesis of isoamyl acetate through esterification reaction.

The invention belongs to the technical field of biochemical engineering, and particularly relates to a method for preparing medium-chain fatty glyceride through double-enzyme catalysis. The method provided by the invention comprises the following steps: firstly, catalyzing raw materials such as coconut oil to be completely hydrolyzed by utilizing lipase, and separating medium-chain fatty acid with the purity of more than or equal to 99.5% by adopting a molecular distillation cooling crystallization technology; then, high-purity fatty acid and glycerol are used as substrates, immobilized lipase is used as a catalyst, directional esterification is performed in a reactor provided with a dehydration membrane block, and the water activity of the system is controlled to be 0.1-0.3. The content of medium-chain fatty glyceride in the final product is higher than 98%, and the final product does not contain long-chain fatty acid impurities. The process is green and energy-saving, the catalyst can be recycled, the economical efficiency is good, and an innovative solution is provided for large-scale production of the high-purity structured lipid.

The invention belongs to the technical field of grease deep processing and enzymatic modification, and particularly relates to a method for continuously preparing medium-chain and long-chain triglyceride through an enzymatic method. According to the method, medium-chain triglyceride (MCT) and long-chain triglyceride (LCT) are taken as substrates, are mixed and then are introduced into a packed bed continuous flow reactor filled with micron-sized immobilized lipase particles for efficient and controllable transesterification reaction, so that continuous synthesis of the MLCT is realized. And performing low-pressure distillation and molecular distillation combined separation and purification on the obtained product, and performing flavor compounding regulation and control to obtain the MLCT edible oil with both nutrition and flavor. The method has the advantages of being short in reaction time, stable in process, suitable for large-scale continuous production and the like, and an innovative solution is provided for green manufacturing of high-quality flavor type medium-chain and long-chain triglyceride.

The invention discloses hollow mesoporous silica nanoparticle immobilized lipase as well as a preparation method and application thereof in preparation of diglyceride oil. The preparation method comprises the following steps: firstly, preparing a spherical silicon dioxide nanoparticle core as a template, mixing the template, a surfactant, a silicon source and a basic catalyst, carrying out self-assembly and hydrolysis through an intermolecular force, and removing the surfactant to obtain the hollow mesoporous silicon dioxide nanoparticle. Lipase is immobilized on the hollow mesoporous silica nanoparticles in a covalent cross-linking mode, and the hollow mesoporous silica nanoparticle immobilized lipase is obtained. The immobilized lipase has remarkable advantages in the aspects of enzyme catalytic activity retention, enzyme loading rate, mechanical stability and the like, and is good in repeatability and practicability. According to the application of the hollow mesoporous silica nanoparticle immobilized lipase in preparation of diglyceride oil, high-content diglyceride can be obtained, and the hollow mesoporous silica nanoparticle immobilized lipase is high in catalytic efficiency, stable and convenient to recycle.

The invention provides a preparation process and application of diglyceride oil. The preparation method of the diglyceride oil comprises the following steps: firstly, carrying out hydrolysis reaction on plants and water under the action of immobilized lipase, carrying out molecular distillation on a product, regulating the temperature of molecular distillation to be 120-160 DEG C and the vacuum degree to be 10-12Pa, and further carrying out esterification reaction on obtained fatty acid and glycerol under the action of the immobilized lipase, so as to obtain the diglyceride oil. And carrying out molecular distillation on the product, and regulating the temperature of molecular distillation to be 120-180 DEG C and the vacuum degree to be 5-12Pa, so as to finally realize the preparation of the high-purity diglyceride oil. The preparation process disclosed by the invention is green, efficient, strong in enzymatic specificity, high in raw material conversion rate, high in diglyceride content, high in purity and extremely low in byproduct content, has excellent stability and functional activity, can be widely applied to the fields of functional foods, health care products or medicines, and has a good industrialization prospect and a wide market application value.

The invention discloses a catalytic synthesis method of glycerol monolaurate diacrylate, which adopts an enzyme catalysis-chemical catalysis step-by-step method and comprises the following steps of: firstly, synthesizing glycerol monolaurate by reacting glycerol and lauric acid under the catalysis of immobilized lipase, and then synthesizing glycerol monolaurate under the protection of nitrogen in the presence of a polymerization inhibitor. And lauric acid monoglyceride and acrylic acid are catalyzed by the solid acid to generate esterification reaction to generate lauric acid monoglyceride diacrylate. According to the method, glycerol and lauric acid are catalyzed by immobilized lipase to synthesize glycerol monolaurate in a green and efficient manner, primary hydroxyl esterification of glycerol is specifically catalyzed to generate 1 (3)-glycerol monolaurate, secondary hydroxyl almost does not react, and generation of diester byproducts is avoided as much as possible; the solid acid realizes GML dihydroxyl high-selectivity esterification under mild conditions, meanwhile, the double-bond activity of acrylic acid is protected, the double-bond retention rate is greater than 93%, and a high-purity monomer is provided for a UV curing material.

The invention discloses a method for synthesizing retinoic acid ceramide ester through enzyme catalysis and application of the retinoic acid ceramide ester, and belongs to the technical field of cosmetic raw material synthesis. According to the method, retinoic acid and ceramide NP serve as substrates, specific immobilized lipase serves as a catalyst, and in a non-aqueous phase organic solvent, the target product is efficiently synthesized through the steps of raw material preparation, anaerobic reaction system building, directional esterification reaction, aftertreatment and purification detection. The method solves the problems of low substrate solubility, more byproducts, difficulty in catalyst recovery and the like in the traditional process, has the advantages of mild reaction conditions, capability of retaining the activity of the raw materials, high product purity and anti-aging and barrier repair effects, and can be widely used for preparing raw materials of anti-wrinkle and repair cosmetics.

The invention belongs to the technical field of tocopherol extraction, and particularly relates to a method for purifying tocopherol based on a solid phase. The invention relates to a method for purifying tocopherol based on solid phase, which comprises the following steps: carrying out microwave assistance on plant deodorized distillate, simultaneously promoting immobilized lipase to catalyze fatty acid esterification reaction by using a water absorbent, and then carrying out cold separation and filtration to remove sterol so as to obtain a primary tocopherol concentrated solution; fatty acid methyl ester is adsorbed through a hydroxypropyl-alpha-cyclodextrin ethanol water solution, fatty acid methyl ester is removed through cold separation and filtration to obtain a secondary tocopherol concentrated acid solution, high-purity and high-yield tocopherol is obtained through purification of an ion exchange column, and the activity of the obtained tocopherol is high. According to the preparation method disclosed by the invention, sterol and fatty acid methyl ester with relatively high purity and yield can be obtained at the same time. The preparation method is mild in condition, part of reagents can be recycled, and environmental pollution is small.

The invention belongs to the technical field of functional grease preparation, and discloses a preparation method and application of medium-chain and long-chain fatty acid triglyceride. The preparation method comprises the following steps: taking mixed vegetable oil; adding water and heating to obtain an oil-water mixture; filling the immobilized lipase into a glass column with external circulating water heating for preheating; injecting the oil-water mixture from the upper part of the immobilized enzyme column to carry out enzyme hydrolysis reaction; collecting a hydrolyzed oil crude product, and separating free fatty acid; taking medium chain triglyceride, mixing, and injecting into the immobilized enzyme column for esterification reaction; and removing reaction by-products to obtain the medium and long chain fatty acid triglyceride rich in omega-3 / 6 / 9 equilibrium proportion. The product prepared by the method contains medium-chain fatty acid and long-chain fatty acid at the same time, the proportion of different fatty acids is more balanced, the health requirement of a human body on fatty acid intake is met, the immobilized enzyme column technology is used in a solvent-free system, the steps of lipase and oil separation are simplified, the service life of enzyme is remarkably prolonged, and the method is suitable for industrial production. The energy consumption and pollution emission are reduced, the production cost is reduced, and the modern industrial green development concept is met.

The invention discloses a bio-enzyme-based polyester plasticizer synthesis method, and relates to the technical field of chemical production, and the method comprises the following steps: mixing binary acid and dihydric alcohol to prepare a mixed base solution; adding an immobilized lipase catalyst into the mixed base solution to prepare a reaction solution; under the protection of nitrogen, feeding the reaction solution into an ultrasonic reactor, and adding a 3A molecular sieve into the reaction solution to prepare a mixed solution; and sequentially carrying out centrifugal separation on the mixed solution prepared in the step 3 to recover the immobilized lipase catalyst, and carrying out reduced pressure distillation to remove unreacted monomers, so as to prepare the polyester plasticizer. Therefore, the aminated molecular sieve is used as a carrier of the Candida antarctica lipase, and high-density enzyme loading capacity is provided.

This invention discloses an oil-based nanostructured lipid carrier based on MLCT, its preparation method, and its application. The method includes mixing long-chain oil with decanoic acid triglyceride, adding immobilized lipase, vacuum drying, stirring, filtering, deacidification, and vacuum evaporation to obtain MLCT-structured lipids. Glyceryl monostearate is mixed with the MLCT-structured lipids and heated to form the oil phase. Trichosanthes kirilowii seed oil is mixed with ultrapure water and heated to form the aqueous phase. The aqueous and oil phases are then mixed, heated, ultrasonically broken up, and subjected to an ice bath to obtain the oil-based nanostructured lipid carrier. This invention replaces traditional small-molecule emulsifiers with natural oil bodies (OB) as emulsifiers, meeting consumer health needs while exhibiting excellent stability and safety; it also reduces the risk of chemically synthesized residues. Furthermore, the introduction of MLCT revolutionizes the digestibility of NLCs at the metabolic mechanism level. In vitro simulated digestion experiments show that the free fatty acid release rate is higher than 80%, providing a new solution for the development of functional food and drug delivery systems.

The invention discloses a polyester spandex regenerated spandex preparation method based on closed-loop circulation, and relates to the technical field of high polymer material recycling, the method comprises the following components: waste polyester spandex pretreatment, depolymerization reaction, immobilized lipase catalytic polymerization and regenerated spandex preparation; by optimizing each reaction step and condition, the conversion rate of the monomer and the quality of the polymerization product are improved, so that the production cost is reduced, particularly in the immobilized lipase catalytic polymerization step, the immobilized lipase is recovered and regenerated, the service life of the enzyme is remarkably prolonged, the catalysis cost is reduced, and meanwhile, the production efficiency is improved. According to the recycling step of depolymerization by-products, for example, heat generated by incineration of filter residues is used for reaction heating, low-boiling-point impurities are used as a depolymerization agent supplementary raw material, energy and raw material cost are further saved, and under the combined action of the measures, the method has higher economic benefits and enhances market competitiveness when regenerated spandex is produced.