520 results about "Immobilized lipase" patented technology

The invention discloses a method for transforming fatty acid ethyl ester into glyceride, which comprises the following steps of: mixing the fatty acid ethyl ester and glycerol in a material tank; making the material pass through a glycerol separator by using a pump to separate free glycerin; then putting the material in a reactor in which immobilized lipase is filled; and making the material passthrough a packed tower to remove ethanol; making the material finally flow back to the material tank for performing 6 to 300h circular reaction; then carrying out molecular distillation on the reaction product to remove the unconverted reactant so as to obtain glyceride products. The method can improve the stability and bioavailability of the products, and has high application value for transforming functional fatty acids such as ethyl ester fish oil, algae oil, conjugated linoleic acid, arachidonic acid and the like into glyceride products.

A composition is provided containing powdered cellulose and at least one water insoluble, immobilized water treatment additive. Preferably, a filter media composition is provided containing powdered cellulose and at least one water insoluble, immobilized enzyme such as lipase for filtration of swimming pool and spa water. The lipase may be recombinantly produced, and lipases having 1,3 positional or non-positional specificity are used. The immobilized lipase may be in the form of bead-shaped particles, and immobilization can be on a macroporous acrylic resin. Moisture may be added to the composition to provide a pre-moistened composition that is dustless and provides for improved dispersement and even filter grid coating. In a filtering system, the filter media absorbs oils contained in pool or spa water, and the lipase hydrolyzes the oils.

The invention relates to a method for preparing glyceride rich in algal oil n-3 polyunsaturated fatty acid through the enzyme process. The method comprises the following steps: taking microbial algal oil as raw material, carrying out hydrolysis of part or all of algal oil respectively through controlling the hydrolysis rate under the catalysis of lipases to obtain glyceride partially hydrolyzed and free fatty acid; enabling the free fatty acid to enrich n-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) through the urea adduct method to obtain an n-3 polyunsaturated fatty acid condensate; and taking the n-3 polyunsaturated fatty acid condensate and glyceride partially hydrolyzed as substrates, taking immobilized lipases as catalysts, and efficiently synthetizing glyceride rich in algal oil n-3 polyunsaturated fatty acid in an organic phase. The invention adopts a complete biological enzymatic enrichment process, the raw material is clean and safe, the process route is scientific and reasonable, the catalytic activity of enzyme is high, the catalyst can be recycled, the cost is low, the reaction condition is mild, the energy consumption is low, the environment is friendly, and the market prospect is wide.

The utility model relates to a preparation method for immobilized lipase. The method comprises the following steps: macroporous resin is added with lipase solution and imprinting molecule after being pretreated, and interface activation technical processing is performed after oscillation and absorption, freezing and drying, activating agent is removed through filtration, then refreezing and redrying are performed, and the immobilized lipase is obtained. The ester exchange reaction experiment indicates that the immobilized lipase prepared by the invention has better catalytic activity and service stability, the conversion rate of the catalytic reaction can be up to 97 percent, and the reused batches are 200 times.

The invention discloses an enzyme-method esterification deacidification technology for rice bran oil with a high acid value. The technology comprises the following steps: step one, obtaining rice bran oil with a high acid value; step two, carrying out enzyme-method esterification deacidification: heating the rice bran oil obtained in the step one under stirring in vacuum, adding glycerin and Lipozyme 435 immobilized lipase, carrying out esterification reactions between rice bran oil and glycerin in the presence of Lipozyme 435 immobilized lipase so as to obtain the de-acidified rice bran oil; step three, obtaining rice bran oil with a low acid value. In the provided technology, under a certain vacuum degree and temperature, the Lipozyme 435 immobilized lipase is used to catalyze the esterification reactions between the free aliphatic acids in rice bran oil with a high acid value and the glycerin so as to reduce the content of the free aliphatic acids, and thus the acid value of the rice bran oil can be reduced to below 3 mgKOH/g. The provided technology has the advantages of safety, environment-friendliness, low cost, and high refining yield, and is suitable for being applied to edible oil production.

The invention discloses a production method for coproducing unsaturated monoglyceride by using a diglyceride enzyme method. The method comprises: (1)adding absolute ethyl alcohol into a natural grease, carrying out an alcoholysis reaction under catalysis of immobilized lipase, making the generation rate of fatty acid ethyl ester to be 5-40%w/w, and removing the residual alcohol to obtain an alcoholysis product; (2) adding 5-15%w/w of glycerin into the alcoholysis product of step (1), then reacting the above mixed substrates in a packed-bed enzyme reactor filled with immobilized lipase under a vacuum condition; and (3) performing molecular distillation on the reaction product of step (2), and separating to obtain a high-temperature fraction which is a diglyceride-containing product and a low-temperature fraction which mainly comprises monoglyceride. The reaction system of the invention needs no water, therefore the problem of product acid value is solved, and then coproduction of monoglyceride is realized; and the glycerin does not need preadsorption processing, and therefore the production operation is simplified.

The invention refers to a method of producing diglyceride, mixing lower alcohol and triglyceride to react under fixed lipase, separating reaction product to get the diglyceride, and at the same time getting the by-product, fatty acid lower alcohol ester; the lower alcohol is monobasic alcohol, C number less than 3, the triglyceride is refined animal oil, or vegetable oil, or microbial oil or their mixture, and the fixed liphase is fixed on carrier by physical adsorption, and covalent cross-linking.

The invention discloses a method of catalyzing and synthesizing alpha-glyceryl linolenate with immobilized lipolytic enzyme in the manufacturing method field of a anti-cancer drug material, which comprises the following steps: making immobilized enzyme carrier including hole enlarge of dielectric hole material and the amido modification; making immobilized lipolytic enzyme, synthesizing alpha-glyceryl linolenate with immobilized enzyme in organic medium. The invention provides a good steady, and SBA-15 still shows regular hexagonal structure after modification, a uniform pore path, a big aperture and meets the need of synthesizing alpha-glyceryl linolenate; improvement of the lipase proper temperature improves the productivity; the product is provided with a light yellow color, a good quality, a good curative effect, enhancing heat stability.

The present invention relates to a TL immobilization enzyme and an application thereof. Specifically the present invention provides a preparation method for an immobilization lipase. The method comprises the following steps: a) carrying out a pre-treatment on a carrier, wherein the carrier is selected from an ion exchange resin or a macroporous adsorption resin having a polystyrene skeleton; b) providing a lipase; c) carrying out contact of the pre-treated carrier from the step a) and the lipase provided by the step b), such that the lipase is immobilized on the pre-treated carrier; and d) drying. The present invention further provides the immobilization lipase prepared by using the method of the present invention and an application thereof. According to the present invention, the immobilization enzyme of the present invention has high activity and stability, and does not generate soap during a process of catalysis of an ester exchange reaction in food industry, operation is simple, and oil processing cost is substantially reduced.

The invention relates to a method for immobilization of lipase, and specifically relates to a method for immobilization of lipase by a white carbon black carrier. The steps include: providing awhite carbon black carrier, enabling white carbon black to contact and a lipase liquid, and making the lipase immobilized on the carrier; and then drying the immobilized lipase. The obtained immobilized lipase had good ester exchange activity, and after ester exchange, oil has a low soap content.

The invention discloses a method for preparing functional grease by enzyme catalysis and modification on tea oil, comprising the following steps of: mixing tea oil and middle carbon chain fatty acid which are used as raw materials in the mole ratio of 1:(1-1):12; adding water accounting for 0.01-2 percent of the weight of the raw materials; not taking organic solvent as a medium in a nonaqueous phase system, passing through a packed column filled with 1,3-specific immobilized lipase in reverse flow way; reacting at 30DEG C-80 DEG C for 0.5h-3h; and separating the product to obtain grease withrapid energy supply and low heat quantity function, wherein in the functional grease, the middle carbon chain fatty acid is mainly positioned on the sn-1,3 site of triglycercide and unsaturated oleicacid of the fatty acid is mainly positioned on sn-2 site of the triglycercide. The invention can achieve favorable effects of maintaining the stable catalytic activity of enzyme and prolonging the service period of the enzyme by adding a proper amount of water in the raw materials.

The invention provides a method of preparing an immobilized lipase. The method utilizes a coupling immobilization technology, that is a method of combination of adsorpting, crosslinking and embedding, to immobilize the lipase. Application of the coupling technology overcomes disadvantages of easily fell lipase and poor recycling rate caused by an adsorption method; the coupling technology has advantages of high bonding capacity of a crosslinking method and a high enzyme activity recovery of an embedding method, enables the lipase combined with a carrier to firmly immobilize on the carrier, and improves a repetition cyclic utilization rate and an enzyme activity. The immobilized lipase prepared by the technology can be used for esterification, transesterification, and ester hydrolysis, so that a conversion rate can reach more 90 %, a reaction time is shortened, a repetition utilization rate reaches more than 100 times, and an enzyme activity is reduced by less than or equal to 5 %. Therefore, the immobilized lipase has an industrial application prospect.

This invention provides a process for preparing immobilized lipase attapulgite, belonging to the technological field of attapulgite utilization and lipase immobilization. The invention utilizes attapulgite and lipase as raw material and comprises the following steps: attapulgite treatment, lipase immobilization by adsorption, using the absorption of lipase as indication, taking regard of the enzyme activity of immobilized lipase, achieving the ideal process of attapulgite treatement and lipase immobilizatin. The invention has beneficial effects of realizeing improved activity, multiple utilization, high utilization ratio and broader range of application of lipase, laying the foundation for lipase immobilization technology. Furthermoer, the invention enlarges the application range of attapulgite, increases the added value of attapulgite and has good guiding effects to use attapulgite as enzyme vectors.

The invention discloses preparation of organic solvent slow release system and its application in enzyme catalysis reaction. It is formed by multi-hole solid matter and liquid state organic matter. The saturated solid matter reacts with vegetable oil under immobilization lipase and solvent at 30 centigrade degree for 12hr. Its product biological diesel oil yield can reach above 90%. Meanwhile the reclaimed immobilization lipase still holds high catalysis energy.

The invention relates to a method for continuous enzymatic synthesis of n-3PUFA glyceride, comprising the following steps: preparing fish oil n-3PUFA (EPA, DHA) and glycerol into reaction liquid according to a certain proportion; then pumping reaction liquid into an enzyme reaction column provided with immobilized lipase through a constant current pump for carrying out reaction for enzymatic synthesis of n-3PUFA; finally realizing continuous enzymatic synthetic production of n-3PUFA by controlling circulation flow velocity of the reaction liquid in the reactor and setting a molecular sieve dehydrater in the reaction system. The n-3PUFA glyceride product prepared by the continuous syhthesis method features an ester yield of 30-50%, a monoester content of 20-30%, a diester content of 50-70%and a trig content of 10-20%. With the method of the invention adopted, recycle of enzyme can be realized, cost can be greatly reduced, production cycle can be shortened, production efficiency can beimproved and the operation is easy, so that the method is of great practical value to industrialized batch production.

The present invention provides a process for producing biological diesel oil by utilizing combination of two kinds of several kinds of lipolases with different catalytic properties. In particular, it can combine the immobilized lipolase with 1,3-position specificity and the immobilized lipolase having not position specificity together and uses them simultaneously, and utilizes the catalytic property of different lipolases to make oil raw material effectively convert into biological diesel oil.

The invention discloses a method of preparing diglyceride, including the following steps: (1) in a raw material vat, adding acyl receptor containing glyceryl, fatty acid donor and hydrophilic carrier silica gel, and stirring; (2) under the catalytic action of immobilized lipase, the acyl receptor reacts with the fatty acid to generate diglyceride and side-products; (3) after vacuum dehydration or lower alcohol elimination, returning the reaction products to the raw material vat to repeat operation in steps (1)-(3); (4) separating. It solves a series of problems caused by the coating of enzyme by glycerol, improving the catalytic effect of fatty acid.

The invention discloses a biological preparation by immobilized lipase-alcohol permeable membrane biological reactor. The process is that lipase is immobilized on surface of alcohol permeable membrane made of hollow fiber by physical absorption, membrane member of immobilized lipase is prepared, then assembled into enzyme-membrane reactor. Oil passes through shell side of membrane member, and low carbon alcohol passes through tube pass of the same, then fatifies them into biological diesel under catalysis of immobilized lipase. Low carbon alcohol selectively permeates hollow fibers to provide low carbon alcohol needed in preparation of biological diesel, so that inhibitory action towards enzyme activity caused by bottom articles is effectively avoided, and biological diesel is efficiently prepared. Moreover, solvent washing and batchwise charging is avoided, which meets the need of green chemistry, and reduces cost, simplify process, realizes continuous production.

The invention discloses a method for producing L-ascorbyl palmitate through non-aqueous phase enzymatic synthesis. The method comprises the following steps of: (1) mixing L-ascorbic acid with palmitic acid in a non-aqueous medium, adding immobilized lipase for catalytic reaction for 20-50 hours; (2) filtering the reaction liquid obtained in the step (1) to remove the immobilized lipase and undissolved L-ascorbic acid and evaporating the obtained filtrate under reduced pressure to remove a solvent; and (3) dissolving the residue obtained in the step (2) with ethyl acetate, washing with deionized water, separating an ethyl acetate phase out, evaporating under reduced pressure to remove the solvent, dissolving the residue with toluene, recrystallizing, centrifuging, washing the obtained substance with normal hexane and drying the obtained undissolved substance in vacuum to obtain an L-ascorbyl palmitate product. The method relates to non-aqueous enzymatic synthesis, has the advantages of high catalytic efficiency, mild reaction condition, low pollution, simple process, readily-available raw materials, high yield and convenience for large-scale production, and is accordant with the practical situation of the food additive production industry in China.

The invention discloses a preparation method of magnetic hydroxyapatite immobilized lipase. The method includes the steps of preparing a magnetic hydroxyapatite carrier so that lipase can be fixed to the carrier in the form of a chemical bond, conducting amino functional decoration on prepared magnetic hydroxyapatite, and immobilizing lipase with glutaraldehyde as cross-linking agent. The method has the advantages that magnetic nanometer particles are wrapped by hydroxyapatite, hydroxyapatite is provided with plentiful ducts, the inner walls of the ducts are provided with plentiful hydroxyl, further decoration of the carrier and immobilization of active substances are facilitated, and therefore the specific surface area of magnetic particles is greatly enlarged, the loading amount of lipase is increased, and the catalytic effect of immobilized lipase is better embodied; meanwhile, due to the good biocompatibility of hydroxyapatite, the catalytic activity of immobilized lipase is brought into better play.

The invention provides a method for preparing glyceride-type fish oil rich in n-3 polyunsaturated fatty acids. The method comprises the following steps: heating and stirring fish oil acetate, short-chain acid glyceride and immobilized lipase under a vacuum condition till complete reaction, and then removing unreacted fish oil acetate to obtain the glyceride-type fish oil rich in n-3 polyunsaturated fatty acids. According to the method provided by the invention, the defect that a reaction can not be smoothly carried out as glycerinum is easy to wrap immobilized enzyme in a traditional method for preparing the glyceride-type fish oil rich in n-3 polyunsaturated fatty acids can be overcome.

The invention relates to a method of microstructure fixation lipase in the filed of enzyme fixation of biology projection. It dissolves hydrophilic film material acetate fiber into dimethyl form amide to prepare for hydrophilic film solution, it lays water repellency politef film on the glass plate, it spread coats hydrophilic film solution on its surface and uses dry-wet changing method to obtain the compensate film which is formed by fine and close hydrophilic layer and porous bleed layer, it uses filtering method and microstructure to achieve enzyme fixation.

The invention discloses a kinetic resolution method, in particular a method for preparing chemical enzyme of (R)-1-(1-naphthyl) ethylamine by resolution, which using 1-(1-naphthyl) ethylamine as a raw material. In the method, kinetic resolution is performed by using 1-(1-naphthyl) ethylamine which is a racemic mixture as a raw material, phenylethyl acetate as an acyl donor and immobilized lipase from Candida Antarctica as a catalyst under the controlled operation conditions that: the temperature is 30 to 80 DEG C, the volume ratio of the raw material to a solvent is 1:(25-50), the mass ratio of the raw material to the catalyst is 1:(0.02-0.05), and the molar ratio of the raw material to the phenylethyl acetate is 1:(1.2-4.0). The method has the advantages of high catalytic efficiency and environment friendliness.

The present invention relates to a preparation method of polyhydric alcohol ester. Said method includes the steps of adopting immobilized lipolase and utilizing monobasic fatty acid and polyhydric alcohol to catalyze synthesis of polyhydric alcohol ester. Said invention also relates to a polyhydric alcohol ester preparation prepared by adopting the invented method and its application as basic oil of lubricating oil.

The invention relates to the technical field of aquatic product processing, and particularly relates to a method for preparing fish oil by virtue of the leftovers of tuna. The specific scheme disclosed by the invention is as follows: the method comprises the following steps of: removing impurities from the leftovers of tuna and cleaning, and then crushing to obtain a feedstock; then performing primary enzymolysis on the feedstock by virtue of immobilized lipase, then performing secondary enzymolysis, filtering the obtained secondary enzymolysis solution to remove residue, performing centrifugal treatment, and separating to obtain lower-layer emulsion and upper-layer crude fish oil; and demulsifying the lower-layer emulsion, and forming the tuna fish oil with the upper-layer crude fish oil. According to the technical scheme disclosed by the invention, the fish oil is prepared by virtue of the leftovers of tuna; and the fish oil is high in extraction rate, complete in active ingredients and high in content, and the method is low in energy consumption and environment-friendly.

Belonging to the technical field of edible oil processing, the invention relates to a method for preparing low/zero-trans fatty acid shortening by enzymatic interesterification. The method of the invention adopts an immobilized lipase Lipozyme RM IM to catalyze lard and palm stearin or extremely hydrogenated oil (including extremely hydrogenated soybean oil, extremely hydrogenated canola oil, extremely hydrogenated palm oil and extremely hydrogenated cottonseed oil) for interesterification so as to prepare shortening. The solid fat content (SFC) of a reaction product under a temperature of 20DEG C is taken as an index, and by systematically studying the influences of temperature, adding amount of enzyme and time on an interesterification reaction, obtaining ideal technical parameters: a reaction temperature of 60-90DEG C, an enzyme adding amount of 6%-12%, and a reaction period of 1h-3h. In the invention, the compatibility and crystal form of a mixture system before and after an interesterification reaction are studied through an isothermal curve and an X-ray diffraction technology. Results indicate that: after interesterification, the compatibility of the mixture system is obviously improved, the crystal form is converted from beta type into beta' type, and the shortening prepared in the invention is superior to the market shortening in terms of application in bread baking. Widening the range of lard application, the method of the invention brings the abundant lard resources in our country into full play.