22 results about "Immobilized lipase" patented technology

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The application discloses a hot air pretreatment method for extracting oat oil and belongs to the technical field of oil extraction. The application solves the technical problems of low extraction efficiency, easy oxidation of oil and large solvent consumption existing in the prior art. The method comprises the following steps: a. oat bran is dried by hot air at 130-150 DEG C for 15-20 min; b. the oat bran is placed in a negative pressure environment, and ethanol vapor flash treatment is performed for 5-15 min; c. the oat bran is mixed with a mixed solvent of petroleum ether and ethanol, immobilized lipase and natural antioxidant are added, and extraction is performed for 8-12 h, and then filtration separation is performed; d. the first residue is mixed with the mixed solvent, extraction is performed for 4-6 h, and then filtration separation is performed; e. the first supernatant and the second supernatant are combined, the solvent is recovered by reduced pressure distillation, and oat crude oil is obtained; and f. the immobilized lipase is recovered. The method is mainly used for efficiently extracting high-quality oat oil from oat bran.

This invention discloses an oil-based nanostructured lipid carrier based on MLCT, its preparation method, and its application. The method includes mixing long-chain oil with decanoic acid triglyceride, adding immobilized lipase, vacuum drying, stirring, filtering, deacidification, and vacuum evaporation to obtain MLCT-structured lipids. Glyceryl monostearate is mixed with the MLCT-structured lipids and heated to form the oil phase. Trichosanthes kirilowii seed oil is mixed with ultrapure water and heated to form the aqueous phase. The aqueous and oil phases are then mixed, heated, ultrasonically broken up, and subjected to an ice bath to obtain the oil-based nanostructured lipid carrier. This invention replaces traditional small-molecule emulsifiers with natural oil bodies (OB) as emulsifiers, meeting consumer health needs while exhibiting excellent stability and safety; it also reduces the risk of chemically synthesized residues. Furthermore, the introduction of MLCT revolutionizes the digestibility of NLCs at the metabolic mechanism level. In vitro simulated digestion experiments show that the free fatty acid release rate is higher than 80%, providing a new solution for the development of functional food and drug delivery systems.

The application belongs to the field of edible oil processing, and specifically discloses a production method of selenium-rich peanut oil system, which comprises the following steps: S1, breaking the selenium-rich peanuts with a seed organic selenium content of greater than or equal to 1.2 mg / kg after pulse strong light enzyme inactivation treatment; S2, releasing oil from the peanut raw materials through low-temperature wall-breaking primary pressing, and then extracting residual oil through gradient temperature secondary pressing; S3, filtering and degumming the crude oil through a ceramic membrane molecular sieve; S4, adding immobilized lipase to the degummed oil for catalytic deacidification; S5, making the deacidified oil flow through an activated carbon fiber membrane for decolorization; and S6, treating the decolorized oil through molecular distillation and deodorization treatment. The application combines pulse strong light enzyme inactivation, gradient low-temperature pressing and membrane integrated refining, controls temperature throughout the process to avoid thermal denaturation, and replaces chemical methods with membrane refining, so that high selenium retention of the selenium-rich peanut oil is achieved, solvent and strong alkali residues are eliminated through physical pressing and enzymatic deacidification, and the working hours and residual oil rate are shortened through membrane refining.

This invention discloses a method for utilizing yellow water and preparing characteristic flavor esters of baijiu (Chinese liquor) based on immobilized lipase, belonging to the field of baijiu biotechnology. This invention constructs a hydrophilic-hydrophobic bifunctional microenvironment on the surface of an inorganic carrier with a mesoporous structure, and activates and immobilizes lipase under mild conditions. The resulting immobilized lipase can efficiently catalyze the esterification reaction of organic acids in yellow water of various baijiu types (light aroma, strong aroma, and sauce aroma) without the addition of external organic solvents, targeting the generation of flavor esters characteristic of baijiu. Through the synergistic effect of the bifunctional surface microenvironment and the immobilization method, the catalytic activity, stability, and reusability of the lipase in complex yellow water systems are improved. The immobilized lipase can maintain stable operation in multiple reaction cycles, significantly extending the enzyme's lifespan and reducing reaction costs. This achieves efficient conversion and high-value utilization of organic acids in yellow water, showing promising industrial application prospects.

This invention relates to the field of lipid biocatalysis and functional lipid preparation technology, specifically to a COF immobilized lipase system for the directed synthesis of sn-1,3-diacylglycerol, its preparation method, and its applications. This invention provides a method and system for regulating the glycerol hydrolysis reaction pathway of lipases using a cooperative covalent organic framework. By introducing multiple functionally complementary and irreplaceable covalent organic framework modules in the same reaction process, it preferentially promotes the conversion of triglycerides to sn-1,3DAG; significantly inhibits the acyl migration of sn-1,3DAG to sn-1,2DAG; reduces secondary non-selective reactions of DAG; and improves the proportion and compositional stability of sn-1,3DAG at the reaction pathway level.

The invention relates to a lipase isolated from the goat gut rumen metagenome having protein SEQ ID 1 of 298 amino acids and being encoded for by gene SEQ ID1. The invention relates more specifically to this lipase being engineered to be an immobilised lipase, the same being immobilised on Polyethylene glycol (PEG) coated iron Magnetic Nanoparticles (MNPs). The immobilised lipase is indicated for use in a method of converting a waste oil to biodiesel, the method comprising utilizing the immobilised lipase to catalyse the conversion of said waste oils to biodiesel in the presence of a short-chain alcohol. The triacylglycerol in the waste oils being converted to alkyl esters (biodiesel) and glycerin in a reaction with an alcohol which is catalysed by said lipases.

The present application relates to the technical field of lutein extraction, and provides a green extraction method of lutein from marigold, which comprises the following steps: mixing marigold dry flower particles with deionized water to prepare slurry, and performing high-pressure homogenization treatment to obtain pretreated slurry; mixing the pretreated slurry with a deep eutectic solvent (DES) and performing countercurrent extraction under the protection of inert gas to obtain DES extraction liquid after filtration; preheating the DES extraction liquid and pumping it into a continuous flow fixed-bed enzyme reactor filled with immobilized lipase, and performing enzymolysis reaction under constant temperature to obtain an enzymolysis liquid; sequentially treating the enzymolysis liquid through a ceramic ultrafiltration membrane and a nanofiltration membrane; collecting the retentate of the nanofiltration membrane, adding preheated deionized water to the nanofiltration retentate, and performing low-temperature standing crystallization; and washing and vacuum freeze-drying the obtained crystals to obtain a lutein crystal product. Through the above technical scheme, the extraction rate of lutein is improved, and the problem of incomplete extraction caused by insufficient cell structure damage in the traditional extraction method is improved.

The application belongs to the technical field of pet food, and provides application of multifunctional poultry glycerol diester, pet food and a preparation method thereof, and a poultry meat paste preparation step to obtain S1, raw material pretreatment: shred raw chicken meat and / or raw duck meat to obtain a meat paste; S2, enzyme catalysis reaction: add immobilized lipase and a reaction medium to the meat paste obtained in step S1, the reaction medium is glycerol, the amount of immobilized lipase added is 3% to 6% of the mass of the meat paste, and the amount of glycerol added is 10% to 20% of the mass of the fat in the meat paste; after uniform mixing, constant temperature stirring reaction is carried out at a stirring speed of 60 to 80 r / min and at 40 to 55 DEG C for 6 to 8 h; S3, enzyme inactivation treatment: after the reaction in step S2 is completed, the material is heated to 80 to 85 DEG C, and kept at this temperature for 15 to 20 min to completely inactivate the immobilized lipase, and poultry glycerol diester poultry meat paste is obtained. Compared with the prior art, the application has the characteristics of realizing weight control, improving skin and fur health and excellent palatability, and has multifunctionality and feeding effect, and is safe and low in production cost.

This invention discloses an immobilized lipase for selectively catalyzing the synthesis of 1,3-dAG, its preparation method, and its application in skincare products. The preparation method of the immobilized lipase includes the following steps: (1) dissolving magnesium salt in a solvent, adding lipase and mixing evenly, then adding 2,5-dihydroxyterephthalic acid (DHTA) and polyvinylpyrrolidone (PVP), stirring and adding concentrated ammonia dropwise, adjusting the pH of the system to 9-11, heating the reaction, and collecting the solid by centrifugation. (2) washing the obtained solid material stepwise with non-polar and polar solvents, centrifuging after each washing, and finally drying the solid to obtain the immobilized lipase. Then, mixing vegetable oil and glycerin, adding solvent under stirring, mixing evenly, adding the prepared immobilized lipase, stirring the reaction, filtering, and evaporating the solvent from the filtrate to obtain the 1,3-DAG product. Using it as a base for skincare products can significantly improve the stability and skin affinity of cosmetics.

This invention provides a method for preparing immobilized lipase using composite-modified protein amyloid protofibrils. The method first prepares β-lactoglobulin amyloid protofibrils by controlling pH and hydrothermal conditions, then polymerizes them into COF protofibrils via TFB and DB condensation. An immobilized carrier is obtained by composite modification with sulfopropyl betaine and pentamalloylmannose to adsorb and immobilize the lipase. In this invention, sulfopropyl betaine anchors to the carrier surface through electrostatic, hydrogen bonding, and hydrophobic interactions, forming a hydration film to prevent pore blockage and enhance the carrier's resilience. Pentamalloylmannose permeates the pores to form a stable interpenetrating structure, giving the carrier a high specific surface area and stability. Furthermore, it stabilizes the loaded lipase through multiple interactions, preventing conformational damage and detachment of the enzyme, while simultaneously creating a stable microenvironment for the lipase, inhibiting oxidation and denaturation inactivation. The immobilized lipase prepared by this invention exhibits high catalytic activity, excellent substrate mass transfer efficiency, and good reusability, and can efficiently catalyze oleic acid esterification reactions.

This invention discloses a series of naringin ester derivatives, their enzyme-catalyzed preparation methods, and applications. Using naringin as a substrate, an immobilized lipase... IM Under TL catalysis, esterification with corresponding vinyl ester acyl donors is carried out in an organic solvent, followed by separation and purification after the reaction. This invention is the first to prepare a series of naringin ester derivatives with different chain lengths via enzymatic catalysis. This preparation method is mild, highly regioselective, has a high conversion rate (>99%), and is easy to scale up for production. Compared to naringin, the naringin ester derivatives exhibit better activity against *Porphyromonas gingivalis* (…). Porphyromonas gingivalis Bacterial gingival protein K (KGP) and its virulence factor exhibit significantly enhanced inhibitory activity, effectively disrupting bacterial cell membranes, inhibiting biofilm formation, and inducing bacterial apoptosis. They have broad application prospects in the preparation of oral care compositions or drugs for the prevention or treatment of periodontitis.

The application discloses a method for synthesizing ceramide ester of retinoic acid by catalysis of an enzyme and application thereof, and belongs to the technical field of cosmetic raw material synthesis. The method uses retinoic acid and ceramide NP as substrates, adopts specific immobilized lipase as a catalyst, and efficiently synthesizes a target product in a non-aqueous organic solvent through the steps of raw material preparation, an oxygen-free reaction system building, directional esterification, post-treatment and purification detection. The method solves the problems of low substrate solubility, many by-products and difficult catalyst recovery in traditional processes, has mild reaction conditions, can retain the activity of raw materials, has high product purity, has the effects of anti-aging and barrier repair, and can be widely used in the preparation of anti-wrinkle and repair cosmetic raw materials.

The application discloses a kind of green enzymatic polycondensation synthesis polyamide 6,5 method, with dimethyl glutarate and 1,6-hexanediamine as raw material, using Novozym435 immobilized lipase as biological catalyst, by direct enzymatic polycondensation reaction preparation biobased polyamide 6,5.The method uses toluene as reaction medium, combined with vacuum by-product, molecular sieve water absorption balance control strategy, realizes the green efficient synthesis of polyamide 6,5, product has excellent thermal stability (melting point 221 DEG C, maximum decomposition temperature 434 DEG C), suitable for melt spinning and other industrial processes, compared with traditional chemical synthesis method, greatly reduce energy consumption, reduce the use of toxic chemicals, provide a new way for the green manufacturing of polyamide.

The application relates to the technical field of renewable bioenergy, in particular to an enzyme catalysis synchronous desulfurization process for preparing bio-diesel from kitchen waste oil, which comprises the following steps: putting pretreated kitchen waste oil, immobilized lipase, a cross-linked chitosan porous microsphere composite adsorbent loaded with zinc oxide and iron oxide and methanol into the same reactor, and performing one-pot ester exchange reaction under the condition of 40-50 DEG C through the mode of step-by-step addition of methanol, so that deep desulfurization is synchronously completed in the reaction process, that is, the core contradiction that the existing desulfurization scheme cannot be applied to the one-pot process due to the incompatibility with the enzyme is solved.

The application discloses a preparation method of immobilized lipase and application of the immobilized lipase in catalysis of 1,3-glycerol diglyceride synthesis. The preparation method comprises the following steps: (1) preparation of a template; (2) immobilization of lipase; and (3) removal of the template. The prepared immobilized enzyme is used for catalyzing conversion of TAG of vegetable oil into DAG, and the selectivity of 1,3-DAG can be remarkably improved, and the proportion of 1,3-DAG in the product is 80% to 90%.

The application relates to the technical field of by-products processing, and particularly discloses a modified immobilized lipase and application thereof in preparation of sauce. The application uses salmon bone row processing by-products, i.e. bone residues, modifies the bone residues, and adopts an adsorption-embedding combined method to immobilize lipase by taking the modified bone residues as a carrier, so that the enzyme activity recovery rate, stability and repeatability of the immobilized lipase are effectively improved. The modified immobilized lipase is combined with a compounded protease, so that the enzymolysis time can be shortened, the amino acid nitrogen level can be improved, and the production cost can be significantly reduced.

The application relates to the technical field of inks, in particular to a high-flexibility acrylic epoxy resin for inks and a preparation method thereof. The preparation method of the high-flexibility acrylic epoxy resin for inks comprises the following steps: synthesizing an acrylic copolymer, carrying out a catalytic reaction of immobilized halogen alcohol dehalogenase, adding a flexible modifier, catalyzing by immobilized lipase and carrying out a grafting reaction of an epoxy resin. The application introduces long flexible side chains by enzyme-catalyzed open-loop grafting of flexible segments, improves the activity of molecular segments on the basis of retaining the original reaction activity and skeleton strength of the acrylic epoxy resin, improves the packing density of the long flexible side chains, improves the activity of molecular segments, and when the prepared acrylic epoxy resin is used in inks, the flexibility of ink films can be improved, the curing speed and surface hardness can be considered, meanwhile, the acrylic epoxy resin does not cause adverse effects on the transparency and storage stability of the ink films.

This invention relates to the field of oil processing technology, specifically to a method for eliminating glycidyl esters in diglyceride vegetable oil. The method includes: Step 1: heating crude vegetable oil to 70-80°C and mixing it evenly with silica, then maintaining the temperature to obtain a first mixture; Step 2: reacting the first mixture, a diluted glycerol solution, and immobilized lipase in a vacuum environment to obtain a first reaction solution; Step 3: adding a polar solvent to the first reaction solution and then sequentially performing primary filtration, cooling, and secondary filtration to obtain diglyceride vegetable oil. This invention utilizes silica to adsorb impurities in crude vegetable oil, and reacts the silica-containing first mixture, the diluted glycerol solution, and the immobilized lipase in a vacuum environment, thereby lowering the reaction temperature and promoting the forward enzymatic hydrolysis of glycerol.

The application discloses an enzymatic synthesis method of diglyceride, comprising the following steps: culturing Pichia pastoris engineering bacteria with high yield of short-chain fatty acid to obtain fermentation liquor containing short-chain fatty acid; obtaining crude extract after acidification and extraction of the obtained fermentation liquor containing short-chain fatty acid; and performing catalytic esterification reaction on the crude extract and glycerol as substrates in a solvent-free system to obtain a crude product containing diglyceride; wherein the crude extract is a mixture of isobutyric acid, iso-valeric acid and 2-methyl butyric acid; and the immobilized lipase is Lipozyme 435. It is found for the first time that the catalytic efficiency of Lipozyme 435 can be greatly improved by directly using the fermentation crude extract containing short-chain fatty acid (instead of high-purity chemicals) as a substrate.

The invention provides a method and system for the enzymatic transesterification of fatty acids or fatty acid derived products to produce lubricant esters. The process involves a reaction mixture containing a fatty acid or fatty acid-derived substrate, an alcohol and a lipolytic enzyme catalyst. A reactive column with a porous frit is employed through which air is bubbled into a mixture of fatty acid derivatives, alcohol and an immobilized lipase catalyst. The air bubbles promote mixing and the subsequent stripping of volatile by-products, thereby increasing ester formation. This process can be carried out at ambient temperature, without the need for external heating or microwave irradiation. The method achieves greater that 99 mol % conversion to ester products at lower energy when compared to conventional thermochemical transesterification.

The present application relates to the technical field of feed additive, in particular to a feed additive for enhancing immunity of ruminants and a preparation method thereof.The present application first reacts L-ascorbic acid with sodium trimetaphosphate to prepare an ascorbic acid phosphate intermediate, then esterifies and calcium-magnesium salts the ascorbic acid phosphate intermediate with lauric acid esterification and calcium-magnesium saltification under the catalysis of immobilized lipase to obtain a phosphorylated-lauric acylated calcium-magnesium ascorbate; subsequently, short-chain chitosan dispersion, first sodium phytate segment locking solution, long-chain chitosan dispersion and second sodium phytate segment locking solution are sequentially added to form modified ascorbic acid immune enhancement microparticles, which are then mixed with pregelatinized corn starch and malt dextrin and spray dried; the present application is conducive to improving the granulation stability, rumen retention and later-stage release and utilization of ascorbic acid active substances, and is suitable for ruminant immune stress period diet addition.