The invention belongs to the technical field of biological
medicine, and particularly relates to a diglycerol
linolenate galactoside compound in pepper as well as a preparation method and application of the diglycerol
linolenate galactoside compound. The preparation method comprises the following steps: (1) taking
red peppers to prepare pretreated
red peppers; (2) adding the pretreated
red peppers into an
ethanol solution, performing ultrasonic extraction, and collecting first filtrate and first filter residues; (3) adding an
ethanol solution into the first filter residue, carrying out ultrasonic extraction, combining the first filtrate and the second filtrate, and concentrating to obtain a crude extract; (4) separating the crude extract by adopting high-speed counter-current
chromatography to obtain a primary purified product; and (5) further separating and purifying the primary purified product by adopting
high performance liquid chromatography, and identifying to obtain the diglycerol
linolenate galactoside compound. The purity of the diglycerol linolenate galactoside compound obtained by the preparation method is as high as 99.3%, and the diglycerol linolenate galactoside compound has a remarkable
scavenging effect on
DPPH free radicals, shows good
antioxidant activity and has a wide application prospect.