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79results about How to "Enzyme stability" patented technology
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Production of biological diesel oil by fixed enzyme method
ActiveCN1818026AEnzyme stabilityExtended half-lifeBiofuelsLiquid hydrocarbon mixture productionOil and greaseBiodiesel
A process for producing biological diesel oil by fixed enzyme method is carried out by taking sodium alginate, kaoline, glutaraldehyde, gelatin and lactose as co-fixer, fixing the lipase, piling the spherical fixed enzyme in filled bed columnar reactor naturally, alcoholyzing animal and plant oil fatty with biological diesel oil as solvent and producing biological diesel oil. It is cheap, has less consumption and by product and better quality. It can be used for continuous production.
Owner:茂名市泓宇能源科技有限公司
High-yield strain of high temperature resistant 1,4-beta-D-xylanase, method for producing high temperature resistant 1,4-beta-D-xylanase through fermentation of high-yield strain, and high temperature resistant 1,4-beta-D-xylanase
ActiveCN102321558AGood stabilityBroad application prospectsBacteriaMicroorganism based processesLaboratory cultureSingle component
The present invention discloses a high-yield strain of high temperature resistant 1,4-beta-D-xylanase, and a method for producing the high temperature resistant 1,4-beta-D-xylanase through fermentation of the high-yield strain, and provides partial enzymatic properties of the high temperature resistant 1,4-beta-D-xylanase. After the strain identification, the high-yield strain is named Paenibacillus campinasensis G1-1, and is preserved in the China general microbiological culture collection center. The preservation number of the strain is CGMCC No. 5023. According to the present invention, the high temperature resistant 1,4-beta-D-xylanase produced through the fermentation of the high-yield strain is subjected to separation and purification to obtain a single component, wherein the single component has a relative molecular weight of 41.3 KDa, an optimal operation temperature of 60 DEG C and an optimal operation pH of 7.0; the relatively stable enzyme activity of the high temperature resistant 1,4-beta-D-xylanase is remained in the temperature range of 40-70 DEG C and the pH range of 5.0-9.0; the Paenibacillus campinasensis G1-1 provided by the present invention is applicable for a plurality of fields such as paper making, food, feedstuff and the like, and has a broad application prospect.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Preparation method of immobilized beta-glucosidase
The invention discloses a preparation method of immobilized beta-glucosidase in the field of enzyme preparations. According to the method, fructose is adopted as a pore-foaming agent, and the immobilized beta-glucosidase is prepared through the tetraethoxysilane hydrolysis reaction sol-gel method. Different immobilized beta-glucosidase samples are prepared by adding fructose water solutions with different concentrations and beta-glucosidase of different dosages. For the obtained immobilized beta-glucosidase, the invalid adsorption in the enzymolysis process of cellulose can be avoided, so that the enzymolysis conversion efficiency of cellulose is obviously improved, the immobilized enzyme has the advantages of good mechanical strength and multiple times of recycling, and the hydrolysis and saccharifying costs of cellulose are reduced.
Owner:PETROCHINA CO LTD
Enzyme-producing microorganism used for hydrolyzing D,L-pantoyl lactone and application and sifting method of enzyme-producing microorganism
ActiveCN108004291AImprove permeabilityEnzyme stabilityFungiMicroorganism based processesMicroorganismPantoyl lactone
The invention provides an enzyme-producing microorganism for hydrolyzing D,L-pantoyl lactone and application and a sifting method of the enzyme-producing microorganism. The method specifically comprises the steps of using an enzyme-producing microorganism strain to hydrolyze D,L-pantoyl lactone, then acidizing D,L-pantoyl lactone to form a converting solution, and conducting high-performance-liquid chromatography chirality online detection on the formed converting solution to quickly sift out strains which have the catalytic activity and stereoscopic selectivity on hydrolyzing D,L-pantoyl lactone. Through the directed sifting method, it is found that a bacterial strain Fusarium verticillioides CGMCC NO.14552 has an obvious and unique advantage in the aspect of hydrolyzing D,L-pantoyl lactone, the hydrolyzing conversion rate of 1-70% D,L-pantoyl lactone within 5-10 h can reach 35% or above, and the ee value of a target product D-pantoyl lactone is larger than 98%.
Owner:ZHEJIANG NHU CO LTD +3
Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof
ActiveCN103272477AShort adaptation periodHigh efficiency of hydrogen sulfide degradationDispersed particle separationAir quality improvementPorosityEcological environment
The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Liquid fermentation method for producing salt-tolerant cellulase from sea aspergillus niger
InactiveCN102010855AEnzyme stabilityMedium simpleFungiMicroorganism based processesPotato dextrose agarSeawater
The invention discloses a liquid fermentation method for producing salt-tolerant cellulase from sea aspergillus niger. The liquid fermentation method comprises the following steps: 1) screening soil in 10-20m depth of coastal area of the East China Sea to obtain a strain of sea aspergillus niger; 2) inoculating the sea aspergillus niger into a PDA (potato dextrose agar) culture medium, culturing at 32-40 DEG C for 60-90 hours to obtain slant spores, and preserving the slant spores at 4 DEG C; 3) inoculating the slant spores on a PDA-1 culture medium, and culturing at 32-40 DEG C for 60-90 hours to obtain activated spores; 4) inoculating the activated spores on a slant of an eggplant bottle, and culturing at 42 DEG C for 74 hours to obtain eggplant bottle slant spores subject to expanding culture; and 5) utilizing sterile artificial sea water to dilute the eggplant bottle slant spores obtained by expanding culture to 108 spores / ml, inoculating on a liquid fermentation enzyme productionculture medium, and fermenting for 5-10 days to obtain the cellulase. The liquid fermentation method has the advantages that the produced enzyme has high activity, stability, good salt tolerance and the like, and the produced cellulase can degrade cellulose under the condition of higher salt content, thereby laying a foundation for follow-up application of hydrolysate.
Owner:ZHEJIANG UNIV
Feed compound enzyme containing neutral protease and preparation method thereof
The invention discloses a feed compound enzyme containing neutral protease and a preparation method thereof, and belongs to the technical field of preparation of an enzyme preparation. The feed compound enzyme containing neutral protease is prepared from concentrated malt enzyme, acid proteinase, neutral protease, acidic xylanase, cellulose, beta-glucanase, pectinase, amylase, seminase, phytase, Chinese herb extracts, a protective agent and an activating agent in a scientific distribution manner. The feed compound enzyme is high in enzyme activity, wide in action condition, strong in stability, and more applicable to feed production. A safe digestive enzyme can be provided for beasts and birds, the digestion burden is relieved, the utilization rate and the growth rate of the raw materials are improved, the environment is protected, joyful natural malt fragrance is brought for the feed, the livestock and poultry appetite is enhanced, meanwhile, the efficacy of the enzyme preparation can be fully played by proper quantity of activating agent under the same condition, the adding amount of the enzyme preparation is saved, the expiration date of the compound enzyme preparation is prolonged by scientific distribution of the Chinese herb extracts, and the immunity of the fed beasts and birds can be improved.
Owner:HUNAN HONGYING BIOTECHNOLOGY CO LTD
Preparation method of porcine pepsin and gastric mucin
The invention discloses a preparation method of porcine pepsin and gastric mucin, and the preparation method is designed for achieving the purpose of overcoming the defects that by using traditional pepsin preparation methods, pepsin is easily degenerated, organic solvents hurts the bodies of operators, and the cost is high, and the like. The method comprises the following steps: carrying out crude extraction on the mucosa of a basilar part of a porcine stomach by using a hydrochloric acid; then, by using the properties that pepsin is high in solubility and stable in enzyme activity in a weakly acidic solution, and gastric mucin is precipitated after meeting a weak acid, extracting and separating porcine pepsin and gastric mucin by using an acidic aqueous solution replacing acetone; and carrying out centrifugation, filtration, ultrafiltration, drying, and the like on the obtained product, so that porcine pepsin and gastric mucin are obtained. In case that the method is adopted for preparing porcine pepsin and gastric mucin, the activity of pepsin is protected, and meanwhile, because no organic solvent is used, the safety factor of operating is improved, and no harm is caused to the bodies of workers; and the cost is reduced, and the production capacity is increased, so that the method has broad market prospect.
Owner:四川德博尔制药有限公司
Recombined lipoxygenase and preparation method thereof
InactiveCN104293805ALow evolutionHigh enzyme purityOxidoreductasesFermentationHydroperoxide lyaseEscherichia coli
The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg / L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.
Owner:NINGBO UNIV
Aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as culture method and application thereof
The invention relates to an aspergillus oryzae strain capable of producing beta-fructofuranosidase, as well as a culture method and application thereof. An aspergillus oryzae FS4 strain is collected in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2014, with the collection number of CGMCC No. 9087 and the address of Institute of Microbiology, Chinese Academy of Sciences, Building 3, NO.1 Beichen West Road, Chaoyang District, Beijing. The invention further relates to the culture method and application of the aspergillus oryzae strain. The aspergillus oryzae FS4 strain obtained by screening has the advantages of simple culture, stable genetic nature, high synthesis efficiency and stable enzyme activity, can be applied to the field of synthesis of levan type oligofructoses and the like by a modern bio-engineering technical enzyme method, and further has broad application prospects.
Owner:SHANDONG UNIV
Bacillus thermophilus capable of producing high-temperature and alkali resistant xylanase and application of bacillus thermophilus
ActiveCN103667151AEnzyme stabilityBacteriaMicroorganism based processesMicroorganismReaction temperature
The invention discloses a bacillus thermophilus 701 capable of producing high-temperature and alkali resistant xylanase and belongs to the technical field of microbial fermentation. The bacillus thermophilus is obtained through the steps that a soil sample is collected from a paper mill, a bacillus thermophilus is separated from the soil sample, and ultraviolet mutagenesis and nitrosoguanidine mutagenesis, and screening are performed repeatedly. The xylanase produced by the bacillus thermophilus has the characteristics of strong high-temperature resistance and alkali resistance. The bacillus thermophilus is preserved in the China Center for Type Culture Collection on October 12, 2013, the preservation No. is CCTCC M2013537. The high-temperature and alkali resistant xylanase prepared from the bacillus thermophilus 701 is 350-420 IU / ml in specific activity, 40-75 DEG C in applicable temperature range, 65 DEG C in optimum reaction temperature, completely stable in enzyme activity at the temperature of 75 DEG C, 5.0-11.0 in applicable reaction pH value, completely stable in enzyme activity at the pH value of 11.0, and is 10.0 in optimum reaction pH value.
Owner:HUNAN HONGYING BIOTECHNOLOGY CO LTD
Biosynthesis method of beta-alanine
InactiveCN108048500AOvercome transmembrane resistanceImprove stabilityMicroorganism based processesFermentationBiotechnologyReaction rate
The invention discloses a biosynthesis method of beta-alanine, which includes reaction of transforming and compounding beta-alanine by recombined brewing yeast full cell catalyzed L- asparaginic acid,wherein the recombined brewing yeast contains exogenous L- aspartic acid-alpha- decarboxylase encoding gene. The method and application have advantages of low-priced and easy-acquired raw materials,simple and easy-controlled operation, green and high-efficient synthetic route, good in biological safety, quick in reaction rate, high in converting rate, and others; the method and application alsolay a foundation of the industrialization of beta-alanine.
Owner:INNOBIO CORP LTD
Novel glucoamylase VGA (video graphics array), gene thereof and application thereof
ActiveCN102382807AEnzyme stabilityImprove high temperature resistanceFungiMicroorganism based processesBiotechnologyGraphics
The invention relates to the field of genetic engineering, in particular to a novel glucoamylase VGA (video graphics array), a gene thereof and application thereof. The novel glucoamylase VGA is provided with an amino acid sequence as indicated in SEQ (sequence) ID (identification) NO.1. The invention further provides a gene encoding the novel glucoamylase VGA, a recombinant vector comprising thegene, a recombinant strain comprising the gene and application of the gene, and a nucleotide sequence of the gene is indicated as SEQ ID NO.2. The enzyme activity of the novel glucoamylase VGA is stable under the condition of 2.0-7.5 pH (potential of hydrogen), and the novel glucoamylase VGA is remarkable in amylolysis effect. In addition, the glucoamylase has better high-temperature resistance, and loss of the enzyme activity of the glucoamylase is lower than 15% after the glucoamylase is treated in 85 DEG C water liquor for 10min, so that the glucoamylase completely meets various industrialrequirements and is suitable for being applied as a novel high-temperature-resistant glucoamylase.
Owner:湖南康捷生物科技有限公司
Enhanced phytase variants
InactiveUS20130122567A1Current limit can be exceededCurrent is limitedAnimal cellsBacteriaFood additivePhytase
The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and / or activity than the original phytase. The invention also provides a nucleic acid coding for said variant, a cassette or an expression vector containing said variant, a host cell expressing said variant, a composition comprising said variant and uses thereof, principally in the preparation of food additives and animal feed.
Owner:BIOMETHODES R L
Bacterial strain producing high-temperature-resistant beta-galactosidase and screening method thereof
InactiveCN105400728AImprove thermal stabilityImprove stabilityBacteriaMicrobiological testing/measurementScreening methodBacterial strain
The invention discloses a bacterial strain producing high-temperature-resistant beta-galactosidase and a screening method thereof and belongs to the technical field of biological chemistry and protein engineering. The screening method includes the specific and sequential steps of sample collection, primary screening, secondary screening of culture and beta-galactosidase activity assay. Mainly, soil of hot spring land is collected and boiled, then bacterial colonies are screened, and the technical problem how to produce high-enzyme-activity beta-galactosidase at high temperature is solved through a high-temperature culturing method. The bacterial strain is short in beta-galactosidase production period, high in beta-galactosidase production speed, good in genetic stability, thermal stability and pH stability, high in enzyme catalytic activity and high in production safety, and culturing conditions are simple. The bacterial strain has broad prospects in the fields of food industry and medical and biochemical analysis, in particular to the dairy industry.
Owner:CHONGQING UNIV
Wheat ration enzyme containing neutral protease and preparation method thereof
ActiveCN103740683AHigh temperature resistanceSuitable for a wide pH rangeClimate change adaptationAnimal feeding stuffCelluloseNeutral protease
The invention discloses wheat ration enzyme containing neutral protease and a preparation method thereof and belongs to the technical field of enzyme preparation. The wheat ration enzyme is formed by scientific compounding of bacillus subtilis culture, acid protease, acid xylanase, cellulose, beta-glucanase, amylase, beta-mannase, Chinese herb extract, protective agent and activating agent. Due to the bacillus subtilis culture, the immunity of organism of animals can be improved, the growth of the immune organ is enhanced, the maturity of the structure and the function of the intestine of the animal is enhanced, the daily gain of the animal is increased and the feed conversion ratio is improved. The wheat ration enzyme disclosed by the invention has the advantages that safe digestive enzyme is provided for livestock and poultry, the digestion burden is effectively reduced, the material utilization rate and the growth rate are increased, and the environment is protected; simultaneously, due to use of the proper amount of activating agent, the effect of the enzyme preparation can be fully played under the equal conditions, so that the adding amount of the enzyme preparation is saved; due to the scientific compounding of the Chinese herb extract, not only the quality guarantee period of the compound enzyme preparation is prolonged, but also the immunity of the raised livestock and poultry can be improved.
Owner:HUNAN HONGYING BIOTECHNOLOGY CO LTD
Process For the Production of an Optically Enriched Tertiary Alcohol
InactiveUS20080299626A1Improve stabilityActivity under process conditionsFermentationEpoxideTertiary alcohols
A process for the production of an optically enriched tertiary alcohol of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with a nucleophilic agent Nu in the presence of halohydrin dehalogenase.
Owner:BASF SE
Cold water low-temperature detergent and preparation method thereof
ActiveCN106929187AEnzyme stabilityPlay a stabilizing effectOrganic detergent compounding agentsSurface-active detergent compositionsSurface-active agentsProtease
The invention provides a cold water low-temperature detergent and a preparation method thereof. Specifically, the cold water low-temperature detergent provided by the invention comprises low temperature protease, an enzyme stabilizer and a surface active agent, wherein the content of the low temperature protease is 10000u / ml-300000u / ml, the content of enzyme stabilizer is 4%-10% (w / v) and the content of surface active agent is 2%-12% (v / v). The cold water low-temperature detergent provided by the invention has the advantages that the enzyme activity of the low-temperature enzyme is stable, the defect of unstable low-temperature enzyme is overcome and the shelf life of the detergent is obviously prolonged.
Owner:上海好福佳洗涤科技有限公司
Method for preparing fibrilia by using alternaria tenuis DB3 strains
InactiveCN102409412AExtensive culture conditionsImprove heat resistanceFungiMicroorganism based processesFiberAlternaria
The invention relates to a method for preparing fibrilia by using epicoccum nigrum DB3 strains. The method comprises the following steps: (1) preserving the epicoccum nigrum DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.
Owner:DONGHUA UNIV
Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger
InactiveCN102010856AEnzyme stabilityExtensive processFungiMicroorganism based processesSporeHydrolysate
The invention discloses a solid fermentation method for producing salt-tolerant cellulase by using an oceanic Aspergillus niger, comprising the following steps of: (1) screening an oceanic Aspergillus niger from soil in the depth of 10-20m approaching to the East China Sea; (2) vaccinating the oceanic Aspergillus niger on a PDA culture medium for culturing to obtain an inclined-plane spore, and preserving the inclined-plane spore at 4 DEG C; (3) vaccinating the inclined-plane spore on a PDA-1 culture medium for culturing to obtain an activated spore; (4) vaccinating the activated spore on an eggplant bottle inclined plane for culturing for 74h at 42 DEG C to obtain an eggplant bottle inclined-plane spore; and (5) propagating the obtained eggplant bottle inclined-plane spore, diluting the eggplant bottle inclined-plane spore to 108 spores / ml by using sterilized artificial seawater, vaccinating to a solid fermentation enzyme-producing culture medium, and fermenting for 8-12 days to obtain the cellulase. The solid fermentation method has the characteristics of coarse culture medium, wide source, low price and the like, the produced cellulase can degrade cellulose under the condition of a higher salt content, and the enzyme is abnormally stable under high-salt environment, therefore, the produced cellulase lays the foundation for the subsequent application of hydrolysate.
Owner:ZHEJIANG UNIV
Preparation method of magnetic-cellulose-microsphere-immobilized lipase catalyst
ActiveCN107326021AHigh loading rateFirmly loadedHydrolasesOn/in organic carrierRegenerated celluloseCalcium carbonate
The invention relates to a preparation method of a magnetic-cellulose-microsphere-immobilized lipase catalyst. The object is to provide a simple, economic, and efficient preparation method of an immobilized lipase catalyst. The adopted process and method comprise: taking cellulose as a raw material, taking solid calcium carbonate particles as a pore-forming agent, through adoption of a hot melt adhesive conversion method and combination with reverse suspension technology, preparing porous regenerated cellulose microspheres, carrying out epoxidation and amination grafting modification, taking micropores as a reactor for a co-precipitation reaction to prepare magnetic cellulose microspheres, and using a covalent bonding method to immobilize lipase molecules to surfaces and micropores of the magnetic cellulose microspheres. The method is abundant and available in raw materials, and the prepared magnetic cellulose carrier is relatively small in size, uniform in distribution, and large in specific surface area. Compared with a catalyst made from a conventional method, the catalyst is high in enzyme immobilization amount, good in dispersion, and firm in loading. The catalyst is convenient to recycle, and can be widely applied to fields of medicine, environment, and energy.
Owner:NORTHEAST FORESTRY UNIVERSITY
Stable bioactive substances and methods of making
InactiveUS20140271605A1Stabilize enzymeEnzyme stabilityPowder deliveryBiocideChemistryBioactive substance
The present invention relates to stable bioactive substances and preparation thereof. A preparation method includes dissolving a bioactive material in a solution substantially free of solutes to make a bioactive solution, adsorbing the bioactive solution onto dry polysaccharide particles to make bioactive loaded polysaccharide particles, and drying the bioactive loaded polysaccharide particles.
Owner:INTERVET INC
Mannanases and recombinant expression bacterial strain thereof
The invention relates to mannanases and a recombinant expression bacterial strain thereof. Expression genes of the mannanases is cloned from penicillium decumbens. Amino acid sequences of the mannanases are shown in the formula of SEQ ID NO: 1. The invention also comprises Pichia pastoris pdman-2 engineering bacteria and Trichoderma reesei pdman-2 engineering bacteria for expression of the mannanases. The novel expression genes of the mannanases are cloned from penicillium decumbens, and the Pichia pastoris pdman-2 engineering bacteria and the Trichoderma reesei pdman-2 engineering bacteria for expression of the novel expression gene are constructed. The mannanase expressed by the Pichia pastoris pdman-2 engineering bacteria has the most suitable pH value of 4.5, the most suitable temperature of 55 DEG C and stable enzyme activity in a pH value of 2.0-7.0, and can resist gastric acid and pepsin. The mannanase expressed by the Trichoderma reesei pdman-2 engineering bacteria has the most suitable pH value of 4.5, the most suitable temperature of 55 DEG C and stable enzyme activity in a pH value of 3.5-8.0, and can resist trypsin. The mannanases have feeding-enzyme application values.
Owner:QINGDAO VLAND BIOTECH GRP
Method for preparing dimethyl carbonate by taking immobilized lipase as catalyst
InactiveCN103525873AEnzyme stabilityHigh activityChemical industryOn/in organic carrierPolyvinyl alcoholTransesterification reaction
The invention discloses a method for preparing dimethyl carbonate by taking immobilized lipase as a catalyst. The method comprises the following steps of immobilizing lipase by taking one or two of polyvinyl alcohol, carboxymethyl cellulose sodium and hydroxypropyl methyl cellulose as a matrix for supporting lipase; then, sequentially adding reactants, namely methanol and ethylene carbonate or methanol and propylene carbonate into a reaction device; adding the immobilized lipase into the reactants; finally, stirring for a transesterification reaction to obtain a product, namely dimethyl carbonate. According to the method disclosed by the invention, by adopting the immobilized lipase as a catalyst to catalyze the transesterification reaction between methanol and ethylene carbonate or methanol and propylene carbonate to obtain dimethyl carbonate, the preparation method realizes high conversion rate, high selectivity and high universality; moreover, the technology is simple, the reaction conditions are mild, the cost is lowered, the method is environment-friendly, the immobilized lipase can be recycled, and the application prospect is broad.
Owner:SHENZHEN LEVEKING BIOLOGY ENG +1
Liquid fermentation method for producing salt-tolerant cellulase from sea aspergillus niger
InactiveCN102010855BEnzyme stabilityExtensive processFungiMicroorganism based processesSporeHydrolysate
Owner:ZHEJIANG UNIV
Recombinant vector containing Jerusalem artichoke fructan 1-exohydrolase gene and application thereof in producing alcohol by fermentation
The invention discloses a recombinant vector containing Jerusalem artichoke fructan 1-exohydrolase gene and application thereof in producing alcohol by fermentation. The recombinant plasmid is prepared by the following steps: inserting a Saccharomyces cerevisiae 3-phosphoglyceric kinase gene PGK1 promoter between EcoRV and SpeI enzyme digestion sites of an expression vector pUG6, inserting a CYC1-terminator-fused Jerusalem artichoke fructan 1-exohydrolase gene Ht 1-FEHs between SpeI and HpaI enzyme digestion sites of the expression vector pUG6, and inserting an 18SrDNA sequence segment of Saccharomyces cerevisiae genome between XbaI and BstEII enzyme digestion sites of the expression vector pUG6. The invention also discloses application of the recombinant plasmid in producing alcohol by fermentation. The Jerusalem artichoke inulase genes Ht-FEHI and Ht-FEHII can promote fermentation of the Saccharomyces cerevisiae to produce alcohol, thereby providing a new method for enhancing the alcohol yield of Saccharomyces cerevisiae fermentation.
Owner:NANJING AGRICULTURAL UNIVERSITY
Suppository for treating mammal endometritis
ActiveCN102552888AIncrease contactProlong the action timePeptide/protein ingredientsSuppositories deliverySodium bicarbonateDecomposition
The invention belongs to the technical field of medicines, and specifically relates to a suppository for treating mammal endometritis. The preparation disclosed in the invention comprises the following components of: by weight, 0.004-0.01% of staphylococcus lysozyme, 0.1-4% of lysozyme, 10-80% of semisynthetic fatty glyceride, 1-20% of poloxamer 188(F68), 1-20% of HPMC, 5-50% of sodium bicarbonate and 5-50% of sodium dihydrogen phosphate. After the preparation provided by the invention enters into mammal uterus through administration, the preparation can undergo effervescence decomposition in a body fluid. The medicine is mixed in foams and rapidly released. Contact area between the medicine and mucosal of uterus becomes larger and the medicine can penetrate into deep portions of mucosa wrinkles so as to prolong the action time between the medicine and the mucosa, raise the concentration of the medicine on local tissues, further enhance the curative effect and effectively kill various pathogens which can cause endometritis.
Owner:昆山博青生物科技有限公司
Clothing stain remover and preparation process thereof
InactiveCN110408481AStrong stain removal abilityEnhanced stain removalOrganic detergent compounding agentsPolymeric surface-active compoundsOrganic acidAlcohol
The invention provides a clothing stain remover and a preparation process thereof. The clothing stain remover is prepared from, by mass, 5-15% of a first surfactant, 5-20% of a second surfactant, 8-15% of 2-methyl-2, 4-pentanediol, 3-10% of polyhydric alcohol, 0.1-0.5% of a chelating agent, 1-3% of organic carboxylates, 1-2% of organic acids, 2-7% of alkylol amine compounds, 2-5% of D-limonene, 0.3-1% of a complex enzyme, 0.05-0.1% of a preservative, 0.02-0.03% of essence and the balance water. According to the technical scheme, the first surfactant and the second surfactant as two nonionic surfactants are combined with 2-methyl-2, 4-pentanediol, polyhydric alcohol, the chelating agent, the organic carboxylates and the organic acids, the combination can make the stain remover have a good stain removing capacity, meanwhile, the enzyme activity of the added complex enzyme can keep stable, and the stain removing capacity is further improved, especially the stain removing capacity of oil stain.
Owner:浙江芬尼奇工贸有限公司
Cotton knitted fabric deoxygenation polishing dyeing-enzyme bath treatment process
InactiveCN103741492AEmission reductionEnzyme stabilityBiochemical fibre treatmentVegetal fibresSucroseWarm water
The invention discloses a cotton knitted fabric deoxygenation polishing dyeing-enzyme bath treatment process, which comprises the following steps: 1) sequentially adding 2-3 parts by mass of ethyl acetate, 35-70 parts by mass of deionized water, 80-85 parts by mass of neutral cellulase, 2-4 parts by mass of a deoxidization enzyme, 30-35 parts by mass of alkyl polyoxyethylene ether, 10-15 parts by mass of sucrose and 15-20 parts by mass of sodium chloride into a reaction kettle for mixing at room temperature, stirring for 55 minutes for preparing a knitted fabric-used enzyme additive; 2) pretreating a gray fabric, then washing in 75 DEG C warm water for 25 minutes, then using nitric acid to adjust the pH to neutral, then adding 2g / L of the cotton knitted fabric-used enzyme additive, running at 65 DEG C for 20-25 minutes, dyeing, then washing with water, washing with soap, dehydrating, and drying to obtain a finished product. According to the cotton knitted fabric deoxygenation polishing dyeing-enzyme bath treatment process, before and during a dyeing course with a reactive dye, the enzyme activity remains stable, so that the process steps are shortened, the energy consumption is reduced, and the discharge of sewage is reduced.
Owner:江苏双圆袋鼠服饰有限公司
Alpha-amylase gene and application thereof
ActiveCN109182304AQuality impactConvenient time controlBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention discloses an alpha-amylase gene. The alpha-amylase gene is prepared from Bacillus sp. 48-1, containing the constructed recombinant vector, the genetically engineered bacterium and the recombinant amylase preparation produced by the genetically engineered bacterium. Having an endotangent. The amino acid sequence of the amylase with alpha-1, 4 glycosidic bond activity is shown in SEQ ID NO: 1, and the coding sequence of the gene is the nucleotide sequence shown in SEQ ID NO: 2. The recombinant vector is constructed and expressed in E. coli, and the product of expression has Alpha-1, 4 endoglycosidic bond function. The present invention describe that amylase plays an important role in promote tobacco leaf alcoholization and the like.
Owner:CHINA TOBACCO YUNNAN IND
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