Cold water low-temperature detergent and preparation method thereof

A detergent and low temperature technology, applied in the field of cold water and low temperature detergent and its preparation, can solve the problems of changing the high-level structure of enzyme protein, poor stability of low temperature protease, unstable enzyme, etc.

Active Publication Date: 2017-07-07
上海好福佳洗涤科技有限公司
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AI Technical Summary

Problems solved by technology

[0004] However, low-temperature proteases have poor stability at room temperature. In enzyme-added detergents, enzymes are usually unstable, and polar solvents, buffers, and various anionic and cationic surfactant components may change the high-level structure of enzyme proteins.

Method used

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  • Cold water low-temperature detergent and preparation method thereof

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Embodiment 1

[0021] The preparation of embodiment 1 cold water low temperature detergent

[0022] 1. Design and screening of short peptide additives

[0023] In order to improve the storage activity of liquid low-temperature protease and improve the quality of the product, we have developed a low-temperature protease inhibitor that can improve the stability of liquid low-temperature protease.

[0024] First, dozens of short peptides were designed according to the cleavage site of low-temperature protease and the three-dimensional structure information of low-temperature protease. The representative short peptides are shown in Table 1, in which the amino acid residues are represented by single-letter abbreviations. The short peptide was synthesized by Gill Biochemical (Shanghai) Co., Ltd.

[0025] 2. Prepare liquid concentrated detergent and test the preservation effect

[0026] An enzyme stabilizer is prepared. The enzyme stabilizer includes: 2 g of calcium lactate, 6 g of 1,2 propylene ...

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Abstract

The invention provides a cold water low-temperature detergent and a preparation method thereof. Specifically, the cold water low-temperature detergent provided by the invention comprises low temperature protease, an enzyme stabilizer and a surface active agent, wherein the content of the low temperature protease is 10000u / ml-300000u / ml, the content of enzyme stabilizer is 4%-10% (w / v) and the content of surface active agent is 2%-12% (v / v). The cold water low-temperature detergent provided by the invention has the advantages that the enzyme activity of the low-temperature enzyme is stable, the defect of unstable low-temperature enzyme is overcome and the shelf life of the detergent is obviously prolonged.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a cold-water low-temperature detergent and a preparation method thereof. Background technique [0002] Compared with traditional detergents, detergents with enzymes as auxiliaries are called enzyme-added detergents. Enzyme-added detergents have stronger decontamination ability and thus have a wider range of applications. Moreover, compared with phosphorus-containing detergents, it is more environmentally friendly, so it can replace phosphorus-containing detergents to meet people's daily and industrial needs. [0003] Protease is currently the most widely used enzyme, and most of the currently used proteases are mesophilic proteases, and the optimum enzyme activity is around 50°C. Low temperature enzymes refer to enzymes whose catalytic temperature is around 30°C and still have a certain catalytic efficiency at around 0°C, mainly produced by low temperature-res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D1/72C11D3/386C11D3/20C11D3/33C11D3/60
Inventor 谢洪斌顾志娟
Owner 上海好福佳洗涤科技有限公司
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