25 results about "Hydrolase Gene" patented technology

The invention relates to the technical field of bioengineering and fine chemical engineering, in particular to epoxide hydrolase, a gene, a carrier, engineering bacteria and application, and the amino acid sequence of the epoxide hydrolase is shown as SEQ ID NO.3. The epoxide hydrolase disclosed by the invention has the advantages that the epoxide hydrolase is Sphingopyis macrogoltabia EY-1, and the structural formula of the epoxide hydrolase is as shown in the specification. According to the invention, a recombinant plasmid pET-28a-smh containing an optimized gene and a genetic engineering bacterium E.coli / smh are constructed, the engineering bacterium is induced to over-express the recombinant epoxide hydrolase SmEH, and the recombinant epoxide hydrolase SmEH has catalytic activity. According to the present invention, the E.coli / smh whole cell is adopted to respectively catalyze the hydrolysis reaction of the (R)-styrene oxide (SO) and the (S)-styrene oxide (SO) so as to obtain the (R)-styrene oxide (SO) and the (S)-1, 2-phenyl glycol (1-Phenyl-1, 2-ethanediol, PED) with high optical purity, and the application of the E.coli / smh whole cell to respectively catalyze the hydrolysis reaction of the (R)-styrene oxide (SO) and the (S)-1, 2-phenyl glycol (1-Phenyl-1, 2-ethanediol, PED) is provided;

The invention discloses an escherichia coli engineering strain with high benzyl acetate yield and application thereof. The escherichia coli strain efficiently expresses a brand new biosynthetic pathway related gene of benzyl acetate; comprising a phenylalanine lyase gene, a cinnamic acid coenzyme A ligase gene, a 3-hydroxyacyl coenzyme A dehydrogenase gene, a 3-oxoacyl coenzyme A ketohydrolase gene, an enoyl coenzyme A hydratase gene, a carboxylic acid reductase gene, a phosphoric acid pantetheinyl transferase gene and an acyltransferase gene. The obtained escherichia coli engineering strain can utilize a sustainable and cheap raw material glucose as a carbon source to realize efficient synthesis of benzyl acetate. The yield of benzyl acetate synthesized by the escherichia coli benzyl acetate engineering bacteria is the highest yield of benzyl acetate synthesized by a microbial fermentation method reported at home and abroad from beginning. The benzyl acetate produced by the engineering strain has the advantages of reliable quality, economic product, green production and the like, and has important economic value and social benefit.

The invention provides a group of recombinant Escherichia coli for forming D-psicose. The recombinant Escherichia coli comprises the following multiple modifications: (a) introducing and expressing a dTDP-glucose-3-epimerase gene, an NDP glycohydrolase gene, an allose-1 phosphate-isomerase gene and an acid phosphatase gene; and (b) enhancing the expression of the glucose phosphate mutase gene, the expression of the glucose 1-phosphate thymine transferase 1 gene and the expression of the glucose 1-phosphate thymine transferase 2 gene. The recombinant Escherichia coli capable of producing D-psicose at high yield is prepared, the yield of by-products is reduced while the yield of a target object is increased, the bottleneck problem in industrial production is solved, and the recombinant Escherichia coli can be used for mass production of D-psicose.

The invention discloses recombinant xanthomonas campestris with xanthan gum hydrolase displayed on the surface and a construction method and application of the recombinant xanthomonas campestris. The recombinant xanthomonas campestris for surface display of xanthan hydrolase is constructed by introducing a recombinant vector obtained by fusion expression of an ice crystal nuclein (INP) gene and a xanthan hydrolase gene into xanthomonas campestris. The gel yield of recombinant xanthomonas campestris fermentation is twice that of a wild strain and reaches 6.7 g / L. The strain can efficiently hydrolyze xanthan gum with high molecular weight (such as more than 100 wDa), the traditional production process which is relatively complicated is simplified, and a new method is provided for large-scale production of the xanthan gum with low molecular weight.

Provided are a method for producing L-glutamic acid and bacteria used therefor. Coryneform bacteria having the ability to produce L-glutamic acid are modified to retain a mutant acetyl-CoA hydrolase gene encoding a mutant acetyl-CoA hydrolase having a substitution of another amino acid residue for the serine residue at position 383 in the amino acid sequence of the wild-type acetyl-CoA hydrolase. The L-glutamic acid is produced by culturing the coryneform bacteria in a medium and collecting L-glutamic acid from the medium and / or the cells thereof.

The invention discloses a hydrolase gene bpfD, an enzyme coded by the hydrolase gene bpfD and application. The strain DN12 is separated and can degrade the BPF and grow by taking the BPF as the unique carbon source and energy. On the basis, a BPF degradation gene cluster bpf is obtained from the strain DN12 by adopting whole genome and comparative transcriptome sequencing analysis. Three different degrading enzymes (two-component oxidase BpfAB, monooxygenase BpfC and hydrolase BpfD) are found in the gene cluster and can catalyze gradual degradation of BPF, and a simple aromatic ring compound p-hydroxybenzoic acid is generated.

The invention discloses a hydrolase gene bitH, a protein coded by the hydrolase gene bitH and application of the hydrolase gene bitH. The full length of the hydrolase gene bitH is 654 bp, the sequence of the hydrolase gene bitH is shown as SEQ ID NO.1, the coded product hydrolase BitH contains 217 amino acids, and the sequence of the coded product hydrolase BitH is shown as SEQ ID NO.2. The BitH can be used for degrading phthalate compounds such as bis (2-hydroxyethyl) terephthalate (BHET), dimethyl isophthalate (DMI) and dimethyl phthalate (DMP). The hydrolase BitH can be used for degrading residues of BHET, DMI and DMP in water and soil, and has very important theoretical and application values.

The invention provides a sclerotinia sclerotiorum mutant strain SEEN10 and application of the sclerotinia sclerotiorum mutant strain SEEN10 in monocotyledon yield increase, and belongs to the technical field of microorganism application. The invention provides a Sclerotinia sclerotiorum mutant strain SEEN10, wherein a gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618), and the gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618). When the mycelium suspension of the strain SEEN10 is applied to seed dressing treatment of wheat seeds, compared with a wild sclerotinia sclerotiorum strain, the yield increase of monocotyledons can be more remarkably promoted, and the pathogenicity of the strain SEEN10 to oilseed rape is greatly reduced. The growth promoting advantage of the strain SEEN10 provides a technical path for green agriculture.

This invention relates to the construction and staining application of recombinant Escherichia coli for synthesizing indoleacetic acid ester. The recombinant Escherichia coli for synthesizing indoleacetic acid ester is obtained by using recombinant Escherichia coli MG1655ΔpheAΔtrpRΔtyrAΔyddGΔarsB::tac70-MaFMOΔldhA::tac70-MaFMOΔyghWX::tac70-tnaAΔpoxB::tac70-cav1 as the initial strain. Then, the endogenous acetylesterase is knocked out, and the endogenous tryptophan hydrolase gene tnaA and the flavin monooxygenase gene maFMO are overexpressed, while simultaneously overexpressing the acetyltransferase gene. The indoleacetic acid ester yield of this invention is as high as 195.3 mg / L. Using indoleacetic acid ester for staining eliminates the need for reducing agents and enzyme solutions, significantly reducing staining costs.

The invention discloses miR-10490-5p for preventing and treating spodoptera frugiperda and application thereof, miR-10490-5p targeting a key gene for growth and development of the spodoptera frugiperda is screened for the first time, and after an analogue agomiR-10490-5p of the miR-10490-5p is injected, it is found that the miR-10490-5p can inhibit normal expression of a juvenile hormone epoxy hydrolase (JHEH) gene of the spodoptera frugiperda, and the miR-10490-5p can inhibit the growth and development of the spodoptera frugiperda. Therefore, the growth and development of the spodoptera frugiperda larvae are slowed down, the feed intake is reduced, and the death rate is increased. In addition, by adding the analogue agomiR-10490-5p into the feed, the growth of the spodoptera frugiperda can be inhibited, the food intake is reduced, and the death rate is increased. The invention provides a new target and technical support for establishing a new strategy for preventing and controlling the spodoptera frugiperda based on the miRNA.

The present invention discloses a hydrolase gene ipcH, the protein encoded thereby, and their applications. The full length of the hydrolase gene ipcH of the present invention is 1983 bp, and the sequence is as shown in SEQ ID NO.1. Its encoded product, hydrolase IpcH, contains 660 amino acids, and the sequence is SEQ ID NO.2. IpcH can degrade the carbamate insecticide isoprocarb. The hydrolase IpcH can be used to degrade the residues of isoprocarb in water bodies and soil, and has very important theoretical and application values.

Provided are a method for producing L-glutamic acid, and bacterium used therein. L-glutamic acid is produced by culturing, in a culture medium, a Corynebacterium having L-glutamic acid-producing ability, the Corynebacterium being modified so as to retain a mutant acetyl-CoA hydrolase gene that codes for a mutant acetyl-CoA hydrolase and having a substitution of a serine residue at position 383 in an amino acid sequence of a wild-type acetyl-CoA hydrolase to another amino acid residue, and collecting L-glutamic acid from the culture medium and / or a bacterial cell.

The present invention generally relates to methods of biological reduction of metal-cyanide complexes after metal-cyanidation and methods of biologically hydrolysing cyanide. More particularly, the present invention allows the engineering of an integrated synthetic lixiviant biological system to be housed within a synthetic host (such as the cyanogenic Chromobacterium violaceum) for efficient precious metal recovery and toxic metal remediation of electronic waste; with up to four main components / modules in the design and engineering of the synthetic host: 1) synthetic cyanogenesis; 2) synthetic metal recovery; 3) synthetic cyanolysis; and 4) synthetic circuits for lixiviant biology. Bacteria capable of reducing ionic metal to ionic metal (such as gold or silver) as nanoparticles, comprising mercury(11) reductase (MerA) comprising a substitution mutation at position V317, Y441, C464, A323D, A414E, G415I, E416C, L417I, I418D, or A422N, are also disclosed. Processes of synthetic cyanide lixiviant production using genetically engineered bacterium transformed with a heterologous hydrogen cyanide synthase gene and a heterologous 3-phosphoglycerate dehydrogenase mutant gene are also disclosed. Processes of synthetic cyanolysis using a genetically engineered bacterium transformed with a heterologous nitrilase gene are also disclosed.

The present invention provides an application of a xyloglucan endotransglucosylase / hydrolase gene, belonging to the technical field of genetic engineering. The application of the xyloglucan endotransglucosylase / hydrolase gene or its related biological material of the present invention includes any one of the following applications: A1. Increasing plant height and increasing plant stem thickness; A2. Cultivating large-plant-type plant lines; A3. Cultivating lodging-resistant plant lines; the nucleotide sequence of the xyloglucan endotransglucosylase / hydrolase gene is as shown in SEQ ID NO.3. The present invention discovers that the xyloglucan endotransglucosylase / hydrolase gene (Nn2g12674) in lotus participates in regulating plant stem development. Overexpressing this gene in rapeseed can significantly increase the plant height and stem thickness of rapeseed, providing new technical guidance for the improvement of plant type and function of lotus and other plants.

The present invention belongs to the field of plant genetic technology, and discloses a new use of S-adenosyl-L-homocysteine hydrolase gene. The present invention reveals that the S-adenosyl-L-homocysteine hydrolase gene Gomus.A12G023400 can be used to improve the fiber quality of cotton or for molecular marker-assisted breeding of high-strength fiber cotton varieties. The technical solution of the present invention provides a new way for molecular marker-assisted improvement breeding of cotton fiber quality.

The invention discloses recombinant corynebacterium glutamicum with hyaluronic acid hydrolase displayed on the surface as well as a construction method and application of the recombinant corynebacterium glutamicum. The recombinant corynebacterium glutamicum with hyaluronic acid hydrolytic activity on the surface is obtained by fusing a hyaluronic acid hydrolase gene with genes of different anchoring proteins by adopting a surface display technology and introducing the fused gene into the corynebacterium glutamicum to carry out surface display, so that the recombinant corynebacterium glutamicum with hyaluronic acid hydrolytic activity on the surface is obtained. The recombinant corynebacterium glutamicum with ultrahigh hyaluronidase activity is obtained through optimization of anchoring protein, connecting peptide, culture conditions and the like, the strain can realize efficient immobilization and reuse of hyaluronidase, meanwhile, the redundant step of enzyme purification is omitted, the production cycle is shortened, and the production cost is reduced. Good application prospects are realized in the fields of cosmetics, medicines and the like.

This application discloses an epoxide hydrolase gene cloned from Angelica dahurica. AdEH1 and AdEH2 and its encoded proteins and applications, among which, AdEH 1 and AdEH2 The nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively, and the amino acid sequences encoding the proteins are shown in SEQ ID NO: 3 and SEQ ID NO: 4, respectively. AdEH1 and AdEH2 The encoded protein possesses epoxide hydrolase catalytic activity, capable of catalyzing the oxidation of imperatorin and angelica root to produce hydrated oxidized imperatorin and angelica root. This application identifies the gene. AdEH1 and AdEH2 Its key role in the biosynthesis of furanocoumarins provides core gene resources for the regulation and green large-scale synthesis of furanocoumarin components in Angelica dahurica, and also provides technical support for the accurate detection of related compounds.

This invention provides a group of recombinant *E. coli* strains capable of synthesizing D-allulose, wherein the recombinant *E. coli* strains include the following modifications: (a) introduction and expression of dTDP-glucose-3-epimerase gene, NDP sugar hydrolase gene, allosugar-1 phosphate-isomerase gene, and acid phosphatase gene; (b) enhanced expression of glucose-1-phosphate thymidine transferase gene, glucose-1-phosphate thymidine transferase 1 gene, and glucose-1-phosphate thymidine transferase 2 gene. This disclosure produces recombinant *E. coli* strains capable of high-yield D-allulose production, increasing the yield of the target compound while reducing the yield of by-products, thus solving a bottleneck problem in industrial production and enabling large-scale production of D-allulose.

The application discloses a glycosyl hydrolase, a gene, a carrier, a host cell and an application. The glycosyl hydrolase is (a) or (b): (a) a glycosyl hydrolase with an amino acid sequence shown in SEQ ID NO:1; (b) a glycosyl hydrolase with equivalent functions derived from (a) by replacing, deleting or adding one or more amino acids in the amino acid sequence shown in SEQ ID NO:1. The glycosyl hydrolase of the application can selectively hydrolyze the glucosyl on the 3rd carbon of PPD type ginsenoside.

The invention discloses a PAEs hydrolase gene and application thereof. The nucleotide sequence of the dibutyl phthalate hydrolase gene paeH is as shown in SEQ ID NO. 1. PaeH (SEQ ID NO.2) expressed by an engineering strain constructed by using the gene can be used for remarkably degrading PAEs (Polyacrylamide) compounds such as dibutyl phthalate, butyl benzyl phthalate, diamyl phthalate, diisobutyl phthalate and the like. Therefore, the produced PaeH enzyme preparation can be used for removing PAEs compounds such as dibutyl phthalate, butyl benzyl phthalate, diamyl phthalate, diisobutyl phthalate and the like which are remained in soil or water.

The invention discloses an application of SCAB75501 and SCAB75511 genes or proteins coded by the SCAB75511 genes in regulation and control of synthesis of indole-3-acetic acid, and belongs to the technical field of gene engineering. According to the invention, a tryptophan monooxygenase gene SCAB75501 and an indole-3-acetamide hydrolase gene SCAB75511 which are positively correlated with the biosynthesis of the indole-3-acetic acid are identified, and the copy number of the SCAB75501 and SCAB75511 genes in streptomyces scabies is increased through a genetic engineering way, so that an indole-3-acetic acid high-yield strain can be obtained; and a technical support is provided for increasing the fermentation yield of indole-3-acetic acid in industrial production. When the copy number of the SCAB75501 gene and the copy number of the SCAB75511 gene are increased in streptomyces scabies 87.22, the yield of the indole-3-acetic acid is greatly increased by 164.2%.

The invention belongs to the technical field of genetic engineering and biological fermentation, and particularly relates to a genetically engineered bacterium, a construction method and application, the genetically engineered bacterium simultaneously contains a dehydrogenase gene and a hydrolase gene, the dehydrogenase is 3-ketosteroid-delta1-dehydrogenase, the nucleotide sequence of the dehydrogenase gene is SEQ ID No.5, and the corresponding protein sequence is SEQ ID No.6; the hydrolase is esterase, the nucleotide sequence of the hydrolase gene is SEQ ID No.7, and the corresponding protein sequence is SEQ ID No.8; according to the present invention, the enzyme liquid of the genetically engineered bacterium is adopted to convert substrates such as hydrocortisone acetate and 6 alpha-methyl hydrocortisone acetate, the conversion is complete within the high substrate content and the short time, the conversion rate reaches 99%, and the genetically engineered bacterium is suitable for industrial production.

The invention discloses miR-13b-5p for preventing and treating spodoptera frugiperda and application of the miR-13b-5p, miRNA and miR-13b-5p targeting a key gene for growth and development of the spodoptera frugiperda are screened for the first time, and after an analogue agomiR-13b-5p of the miRNA and miR-13b-5p is injected, the agomiR-13b-5p is found to be capable of inhibiting normal expression of a juvenile hormone epoxy hydrolase (JHEH) gene of the spodoptera frugiperda, so that the growth and development of the spodoptera frugiperda can be inhibited, and the growth and development of the spodoptera frugiperda can be inhibited. Therefore, the growth and development of the spodoptera frugiperda larvae are slowed down, the feed intake is reduced, and the death rate is increased. In addition, by adding an analogue agomiR-13b-5p into the feed, the growth of the spodoptera frugiperda can be inhibited, the feeding amount is reduced, and the death rate is increased. The invention provides a new target and technical support for establishing a new strategy for preventing and controlling the spodoptera frugiperda based on the miRNA.

A sorbic acid synthetic gene cluster and synthetic method. Provided is any one of the following substances: A1) a polyketide synthase; A2) a biological material related to the polyketide synthase of A1); A3) a hydrolase; A4) a biological material related to the hydrolase of A3); A5) a combination of A1) and A3); and A6) a combination of A2) and A4). A novel sorbic acid biosynthetic gene cluster, which has not been reported in currently disclosed documents, is found, it is the first time that a biosynthetic gene cluster containing a polyketide synthase gene saA and a hydrolase gene saB is heterologously expressed to synthesize sorbic acid, which has reference significance for green biomanufacturing of sorbic acid.

The invention provides a cytidine production strain as well as a construction method and application thereof, the strain is constructed on an E.coliUR12 genome by utilizing a CRIPSR / Cas9 gene editing technology, and a cdd gene and a cmk gene are knocked out; a nucleoside triphosphate reductase gene nrdD is weakened; a nucleoside triphosphate pyrophosphate hydrolase gene nudG, a nucleoside diphosphate kinase gene ndk and a uridine monophosphate kinase gene pyrH (D93A) carrying a mutation point are overexpressed, and a cytidine triphosphate synthase gene pyrG (D160E, E162A, E168K, cgl) of corynebacterium glutamicum carrying a mutation point and a bifunctional nucleotidase gene PHM8 (sce) of saccharomyces cerevisiae are heterologously introduced and multi-copied; the obtained strain is good in hereditary stability and high in fermentation yield, and cytidine can be stably produced.