232 results about "Pesticide degradation" patented technology

The invention provides a quality assurance method by high-quality composite degradation of pesticide residues in orange peels, belonging to the technical field of elimination of the pesticide residues in fruit and vegetable. The method comprises the following steps: selecting different varieties of high-quality orange peels as raw materials; compositely soaking the fresh orange peels in alkali liquor and ozone, and then soaking and washing the orange peels with clear water, and meanwhile carrying out ultrasonic washing during the process; and carrying out low-frequency (915MHz) microwave treatment so that the degradation rate of most part of organophosphorus, organochlorine and carbamate pesticides in the orange peels reaches over 80% and ethion and methamidophos can be fully degraded. The method has the advantages that a plurality of the original pesticide degradation methods are systematically screened and integrated, thus the defect of a single method such as different degradation degrees on different pesticides is avoided so as to eliminate the pesticide residues in the orange peel maximally; and by means of the composite method, loss of nutrition as well as color, aroma and taste components during the degradation process of the pesticide residues in the orange peels is lowered minimally so as to keep the original nutrition, flavor and color of the orange peels maximally.

The invention provides refrigeration equipment which comprises a box body, a pesticide precipitation assembly and a pesticide degradation assembly, wherein a refrigeration space is arranged in the box body in a limiting manner; the pesticide precipitation assembly is arranged on the box body and is used for spraying atomized pesticide educts towards the refrigeration space; and the pesticide degradation assembly is arranged on the box body and is used for spraying pesticide degradation products towards the refrigeration space so as to degrade pesticide precipitated from the pesticide educts. The refrigeration equipment can degrade the pesticide.

The invention discloses a system and a method for purifying soil with pesticide degradation-resistant organic pollutants by using a microbial fuel cell. The system comprises an anode layer (4), a cathode layer (5), an anode conductive material (3), a cathode conductive material (6), a lead and an external resistor (1), wherein the anode layer (4) and the cathode layer (5) are respectively arranged on two sides of a soil layer to be treated; the anode conductive material (3) is arranged in the anode layer; the cathode conductive material (6) is arranged in the cathode layer; the lead is connected with the anode conductive material and the cathode conductive material; the external resistor (1) is connected to the lead. By using the system and the method for purifying the soil with the pesticide degradation-resistant organic pollutants by using the microbial fuel cell, the pesticide degradation-resistant organic pollutants can be efficiently degraded in the soil under the condition that the soil structure is not damaged.

The invention discloses a chlorpyrifos pesticide-degrading bacterium which refers to a Hu-01strain pertaining to Cladosporium sp. The preservation number of the strain is CCTCC M207111, and a Genebank accession number of the ITS sequence analysis is EF405864. Meantime, the invention discloses a method of preparation of inocula. The invention has the advantages of low production cost of inocula, convenient operation and good degradation effect. The invention is suitable for being widely used in fruit and vegetable production and export bases in China or in places possessing green food trademark, which has great significances in promoting the production of uncontaminated vegetable and green food.

The invention discloses an application of bagasse in the preparation of a pesticide degrading bacterium preparation, and provides a pesticide degrading bacterium preparation as well as a preparation method and an application thereof. The pesticide degrading bacterium preparation comprises components as follows: (1), strains having a degrading function on pesticide, such as a Bacilluspumilus HZ-2 strain preserved in the CCTCC (China Center for Type Culture Collection) located in Wuhan University, Wuhan City, Hubei Province on September 6th, 2013, wherein the preservation number is CCTCCNO: M2013380; and (2), a microbial preparation carrier: bagasse. The pesticide degrading bacterium preparation takes bagasse as the microbial preparation carrier and takes glucose or molasses as an auxiliary material, so that the utilization rate of industrial waste is greatly increased, the cost is low, a stable carbon source is provided for microorganisms in the preparation, and the persistent effect of the preparation is guaranteed. The pesticide degrading bacterium preparation is simple to use and good in degrading effect and has good popularization and application values.

The invention discloses a laccase mutant and an encoding gene and an application thereof. The laccase mutant disclosed by the invention is any one of the following protein in a) to d): a) protein as shown in a sequence 1; b) protein as shown in a sequence 3; c) protein as shown in a sequence 5; d) protein with laccase activity and obtained by substituting and / or deleting and / or adding one or multiple amino acid residues in the amino acid sequences of the proteins limited by any one of a) to c). As shown in experiments, compared with wild laccase, the laccase mutant disclosed by the invention has the advantages of high activity, wide pH tolerance range and the like. The laccase mutant disclosed by the invention is expected to be applied in such fields as wastewater treatment in pulp bleaching and dyeing and printing industry, biological detection in medicine field, food industry, organic synthesis, organic pesticide degradation, etc.

The invention relates to a pyrethroid insecticide degradation bacteria separated from Euonymus japonica phyllosphere and a method for preparing a fungicide thereof, belonging to the biotechnology field. By identification, the bacillus is Pseudomonas aeruginosa and is preserved in CCNCC with the preservation number of CGMCC No.2699. The method for producing the fungicide comprises five steps of preparing culture medium, activating strains, preparing liquid seeds, fermenting in a liquid state and preparing the fungicide.The pyrethroid insecticide degradation fungicide produced by the technical scheme of the invention can effectively degrade the pyrethroid insecticides on plant leave surfaces, reduce insecticide residual, ensure food safety and protect ecological environment.

The invention relates to bacillus which is separated from kale phyllosphere and can degrade pyrethroid insecticides effectively, also provides a method for producing the fungicide, belonging to the biotechnology field. By identification, the bacillus is Bacillus subtilis and is preserved in CCNCC with the preservation number of CGMCC No.2698, and the 16S rDNA gene sequence of the bacillus subtilis is submitted to the GenBank database with the accession number of FJ361191. The method for producing the fungicide comprises five steps of preparing culture medium, activating strains, preparing liquid seeds, fermenting in a liquid state and preparing the fungicide. The pyrethroid insecticide degradation fungicide produced by the technical scheme of the invention can effectively degrade the pyrethroid insecticides on plant leave surfaces, reduce insecticide residual, ensure food safety and protect ecological environment.

The invention discloses degrading bacteria for pyrethroids insecticides. The degrading bacteria are HP-S-01 and are identified to be streptomyces (Streptomyces sp.), wherein the collection number of the strain is CCTCC M 2010096; and Genbank access number of 16S rDNA of the strain is HM016873. The invention discloses a method for preparing bactericide by using the degrading bacteria at the same time. The bactericide is low in cost, convenient and desirable in degradation effect, can directly used to reduce cypermethrin pesticide residues in crops by over 80 percent, solves the problem of excessive cypermethrin pesticide residue in the agricultural production, is suitable to be used in national fruit and vegetable production and export bases or places with a green food brand mark on a large scale, and has a great significance for promoting the production of green vegetables and green food.

The invention provides a preparation technology of pure natural nanometer ultrafine calcium powder to solve the problems of easiness in introduction of impurities in the preparation process and large granularity of present calcium powders. The preparation technology of pure natural nanometer ultrafine calcium powder comprises the following steps: 1, selecting a material; 2, preprocessing; 3, cleaning ; 4, crushing; 5, calcining; 6, grinding; 7, adding water to form a uniform aqueous solution; 8, carrying out ultrasonic crushing; and 9, carrying out spray drying. The technology realizes deep processing utilization of shell substances, and a final product obtained in the invention has great market values in the fields of food safety, human body healthcare, medical health, agricultural production and environment improvement due to the unique characteristics of high penetration, sterilization bacteriostasis, pesticide degradation, acid and base balancing, heavy metal removal and mineral substance supplementation and the complete and advanced product structure.

The invention provides a bacteria strain having a high efficiency broad spectrum degradation ability for an organophosphorus pesticide, the strain belongs to Pseudomonas pseudoalcaligenes, and is named as C2-1, the invention also provides a seperation process, cultivation conditions, and a degradation characteristic of the strain, the strain can be grew on an inorganic salt culture medium by using the pesticide. Also provided is seperation and purification of an organophosphorus pesticide degradation enzyme excreted by the strain, and study on enzyme properties, and seperation and clone of genes coding the enzyme. The invention provides a good gene source for cloning organophosphorus pesticide degradation enzyme genes by using a gene project means, finally realizes an object of producing the organophosphorus pesticide degradation enzyme.

The invention discloses a soil pollution control and remediation method which includes the steps: mixing pesticide degradation agents and water to prepare into degradation agent solution according to the weight ratio of 1:(300-500) before planting, uniformly applying the degradation agent solution into soil, applying 3-5kg of soil conditioners, uniformly harrowing the soil, and planting crops; additionally applying 3-5kg of soil conditioners for 1-2 times per mu once during crop growth after planting of the crops, mixing organic water soluble fertilizers and water to obtain organic fertilizer solution according to volume ratio of 1: (1000-1500), uniformly spraying the organic fertilizer solution on the leaf surfaces of the crops for 3-5 times, and spraying the organic fertilizer solution before 7-10 days of harvest again. The consumption of the pesticide degradation agents per mu is 3-5kg, and the consumption of the organic water soluble fertilizers per mu is 30-45ml. According to the method, residual pesticides in the soil are degraded by the aid of techniques such as heavy-metal passivation and microbiological degradation, available heavy metals in the soil are passivated and adsorbed, the moisture and fertilizer retention capacity of the soil is improved, soil fertility is improved, and control and remediation can be achieved according to different pollution conditions of the soil.

The invention discloses construction and application of a broad spectrum organic phosphorus pesticide degradation genetic engineering strain, and belongs to the field of biological engineering technology. The engineering strain 1gmp is the engineering strain paracoccus sp. 1gmp for degrading the organic phosphorus pesticide in a broad spectrum, which is constructed by sequentially inserting two organic phosphorus hydrolase genes mpd and oph with different substrate degradation spectrums into the chromosome of dimethoate high-efficiency degradation strain paracoccus sp. L3 by using random integration of a serious of vectors pUTTns and deleting a resistant gene by counter-selection marking; and degradation spectrum measurement shows that the engineering strain can efficiently degrade 17 organic phosphorus pesticides generally used in China at present. The engineering strain has the advantages of genetic stability, biological safety and great potential application prospect.

A method for biodegradation of a pesticide residual liquid by using edible fungus chaff comprises the following steps: respectively naturally air-drying the edible fungus chaff, surface soil and straw, and then crushing or chopping; mixing the edible fungus chaff, the surface soil and the straw evenly to obtain a mixed matrix, and adding water to make the water content be 60% of the maximal water-holding capacity; curing the mixed matrix for 10 days at the room temperature; filling the cured mixed matrix into a pesticide degradation reactor; arranging the pesticide degradation reactor near a farmland; and evenly spraying a pesticide on the surface of the pesticide degradation reactor, and making the pesticide biodegraded by the edible fungus chaff in the pesticide degradation reactor. The method has the advantages that the method is simple in process, easy to operate, and high in pesticide degradation effect; the used raw materials of the mixed matrix are rich in sources and low in price; and the method not only reduces the effects of pesticide point source pollution on soil and surface water, but also effectively utilizes waste, protects the ecological environment, is obvious in economic and technical effects, and is applicable to vigorous popularization and application.

The invention discloses a manufacturing method of cotton fabric based on light-induced polymer free radicals for degrading pollutants, which belongs to the technical field of functional textiles. According to the manufacturing method, an appropriate H donor agent and a crosslinking agent are selected by utilizing the characteristic that sulfonated polyether ether ketone has high activities to H atoms in organic substances under light induction, so as to cross-link and form a film on the cotton fabric rich in hydroxyl to prepare the self-cleaning fabric which can generate the polymer free radicals by lighting and has an elimination effect on the pollutants. The manufacturing method mainly comprises the following steps: (1) preparing a composite finishing liquid of light-induced free-radical polymer, wherein the finishing liquid contains the sulfonated polyether ether ketone, H donor polymer, the crosslinking agent, HCL (hydrogen chloride) and the like; and (2) padding and baking the cotton fabric in the finishing liquid. The cotton fabric which is prepared by using the preparation method and has a function of degrading the pollutants by the light induction has the effects of antibiosis, pesticide degradation, pigment degradation and the like, can be used for common clothing, medical fabric, household fabric and the like, and has a wide application prospect.

The invention relates to a Fe-based 3 o 4 / CNTs Synergistic Microwave Degradation of Organic Pesticides. The technical scheme adopted is: in the water containing organic pesticide, add catalyzer, irradiate 1-7min under microwave 150-750W; Described catalyzer is Fe 3 o 4 Supported carbon nanotube nanoparticles Fe 3 o 4 / CNTs. The organic pesticide is Diuron or methyl parathion. As a new method for treating organic pesticide wastewater, the invention has the advantages of high degradation efficiency, fast speed, low cost and no secondary pollution, etc., is suitable for large-scale organic pesticide wastewater treatment, and can quickly and completely degrade organic pesticide in a short time.

The invention provides a tea leaf processing method. The method comprises the following steps of fresh leaf selection, pesticide degradation in a spectrum box, green removing, rolling, drying, grading bunching, fermentation, turning and pushing, drying, packing and storing. According to the tea leaf processing method, optical signals of combined spectrum are utilized, pesticide residues in tea leaves can be effectively removed, and the mouthfeel of the tea leaves is obviously improved by synthetically using a reasonable and ordered technology.

The invention discloses a domestic ozone microbubble vegetable and fruit cleaning machine. The domestic ozone microbubble vegetable and fruit cleaning machine comprises an ozone generator and a microbubble generator. The ozone generator generates ozone gas by adopting the principle of high-voltage corona, and the ozone generation concentration is strictly controlled through a precision electromagnetic valve; the microbubble generator generates microbubbles by adopting a gas dissolved and release method, the generated ozone is guided into the microbubble generator and therefore ozone microbubbles are generated. Ozone and microbubbles are combined to generate ozone microbubbles, the ozone dissolution amount in water is increased, and the ozone pesticide degradation, sterilization and disinfection capabilities are enhanced; meanwhile, in the microbubble generation process, a large number of oxyhydrogen free radicals can be generated through stimulation, the ozone oxidability is enhanced, and the good sterilization and disinfection effect is achieved.

The invention discloses streptomyces canus; through identification, it is streptomyces canus, and the bacterial strain name is gl2-18; the streptomyces canus is preserved at China General Microbiological Culture Collection Center; the preservation number is CGMCC No. 13662; the preservation date is February 15, 2017. Compared with the prior art, the resting cell or the growth cell of the new strain streptomyces canus CGMCC 13662 can effectively degrade chloronicotinyl insecticide, in particular to acetamiprid and thiacloprid; the streptomyces canus is used as the pesticide degradation fungicide, and has very good application prospect.

The invention provides application of a microbe strain-Enterobacter aerogenes capable of degrading various pyrethroid pesticides simultaneously to the degrading of pyrethroid pesticide residue, and a preparation for degrading the pyrethroid pesticide residue prepared by the strain. An efficient degrading strain is obtained through screening to simultaneously degrade three pyrethroid pesticide residues, namely bifenthrin, fenpropathrin and cypermethrin, and is analyzed and researched in degrading characteristic, so as to disclose the basic rule of the strain for degrading the pesticide and provide scientific basis for controlling pyrethroid pesticide residue.

The invention provides a soil remediation agent. The soil remediation agent comprises, by weight, 5-15 parts of active components, 5-15 parts of carrier, 10-20 parts of auxiliaries, 35-75 parts of filler and 15-25 parts of water, wherein the active components are bacillus subtilis, Paenibacillus kribbensis, Trichoderma harzianum, Lactobacillus acidophilus and saccharomycetes. The soil remediation agent has the advantages that the pesticide degradation rate of the soil remediation agent after 30 days reaches up to more than 85%, the pesticide degradation rate of the soil remediation agent after 60 days reaches up to more than 94%, and crop yield is increased by more than 40%.

A microbial strain capable of degrading chlorpyrifos pesticide residue in soil with the chlorpyrifos pesticide residue is isolated. The microbial strain is klebsiella sp. dsp-B by identification, Genbank accession number is HQ836365, and the strain preservation number is CCTCC: NO: M2012477. The characteristic of chlorpyrifos pesticide degradation of klebsiella is utilized for development of biodegrading inoculum applicable to the surfaces of garden stuffs and soil. The klebsiella inoculum belongs to the field of microbial environment modification, and the process flow comprises: purified strain preserving, shaking culture, seeding tank enlargement culture, fermentation tank secondary enlargement, and product split charging. The inoculum is simple in preparation technology, low in production cost and easy to popularize and apply, and the application characteristic is direct spraying. The klebsiella inoculum is capable of fast effectively degrading residual chlorpyrifos pesticide on the surfaces of garden stuffs and soil, so that the exceeding standard problem of the residue is solved; and the klebsiella inoculum has direct economical value for production nontoxic nuisance-free greed agricultural products.

The invention discloses a degradation bacterium for pyrethroid insecticides and application thereof. The invention finds out that Staphylococcus succinus has a degradation effect on various pyrethroidinsecticides such as d-cyphenothrin, permethrin, allethrin, bifenthrin, tetramethrin and chlorempenthrin for the first time, and a Staphylococcus succinus strain HLJ-10 which can effectively degradethe pyrethroid insecticides including d-cyphenothrin and the like is obtained by screening. On the basis of this invention, Staphylococcus succinus has a remarkable biodegradation effect on the pyrethroid insecticides including d-cyphenothrin and the like, can effectively degrade pyrethroids in a relatively wide pH value, temperature and concentration range, and can be used for repairing pollutedenvironments including water bodies, soil and the like polluted by the pyrethroid insecticides including d-cyphenothrin and the like. According to the degradation bacterium disclosed by the invention,a new development way is provided for breaking through an existing bottleneck in an existing process for managing pesticide residue caused pollution, a germplasm resources pool of the pesticide degradation bacteria is enriched and the application prospect is wide.

The pesticide degrading agent of esters specially for vegetable and fruit includes cell powder, anionic surfactant, citric acid, sodium bicarbonate and CMCNa. The cell powder is prepared with the microbe strain of the preservation number of CGMCC No. 0290 and through high density culture with detoxicated engineering bacteria to produce detoxification enzyme, fermentation, centrifugation, freeze drying and crushing. The pesticide degrading agent of the present invention can degrade the residual organic phosphate and pyrethrin pesticides in vegetable and fruit with relatively high effect.

The invention discloses a fruit / vegetable cleaning solution, relating to the technical field of daily chemical industry. The fruit / vegetable cleaning solution comprises the following components in percentage by weight: 1-10% of tea bran extracts, 0.01-10% of dry powder of bacterial cells, 20-70% of sorbitol solution, a proper quantity of preservative and the balance of water. The fruit / vegetable cleaning solution has the beneficial effects that the dry powder of the bacterial cells playing a role in degrading pesticides is prepared into the cleaning solution, so that organic phosphate pesticides, organic chlorate pesticides and carbamate pesticides on fruits and vegetables can be degraded, and furthermore the aim of effectively removing residual pesticides is achieved.

The invention discloses a fruit and vegetable cleaning powder, and relates to the technical field of chemicals for daily use. The fruit and vegetable cleaning powder comprises the following raw materials in percentage by weight: 0.2-20% of moso bamboo carbon powder, 0.1-20% of dry somatic cell powder, 0.2-19% of honeysuckle extracting powder, 0.5-19% of scutellaria baicalensis extracting powder, 40-80% of dietary alkali powder and 0.5% of a preservative. The fruit and vegetable cleaning powder has the benefits as follows: the dry somatic cell powder and the dietary alkali powder in the cleaning powder have the effect of pesticide degradation, and can degrade organophosphate, organic chloric acid ester and carbamate pesticides on fruit and vegetables, so that the purpose of effectively removing pesticide residues is achieved; the added moso bamboo carbon powder, the honeysuckle extracting powder and the scutellaria baicalensis extracting powder can clean dirt and bacterial pollutants on the surfaces of the fruit and vegetables.

The invention provides rhodobacter strain and application thereof. The rhodobacter strain is collected in the China Center for Type Culture Collection (CCTCC), and the collection number of the rhodobacter strain is CCTCC No: M 20239. The rhodobacter strain can be used for preparing a pyrethroids pesticide degradation fungicide and has the advantages of low production cost, good pesticide removal effect, high efficiency, no toxicity, environmental protection, and the like. The rhodobacter strain can prepare the pyrethroids pesticide degradation fungicide through a simple, quick and low cost operation method.

The invention relates to a recombinant strain expressing organophosphorus hydrolase, a complex enzyme preparation technology, a complex enzyme preparation and a fruit and vegetable detergent, wherein the recombinant strain comprises a controllable lyase gene. The complex enzyme preparation technology comprises the following steps: culturing the recombinant strain expressing organophosphorus hydrolase, adding proper start-up condition induced expression lyase; and using the obtained culture solution to prepare the complex enzyme preparation. The recombinant strain expressing organophosphorus hydrolase is cultured and the expression of lyase lysed cells is induced, thus the possible influences of complicated mechanical and chemical pyrolysis methods on the expression of enzyme activity can be avoided and the cheap industrialized production is convenient to realize. The complex enzyme preparation produced on the basis of the preparation technology has organophosphorus hydrolase and cell lyase simultaneously, has the double functions of pesticide degradation and sterilization and can be used in the development of the new fruit and vegetable detergent.

The invention provides a microorganism culture that is pseudomonas diminuta and can simultaneously degrade a plurality of pyrethroid insecticides, application of the pseudomonas diminuta in pyrethroid insecticide residue degradation, and a pyrethroid insecticide residue degrading agent that is prepared by the pseudomonas diminuta. The invention obtains the high-efficiency degrading strain which can simultaneously degrade three pyrethroid insecticide residues that include bifenthrin, fenpropathrin and cypermethrin through filtering, and implements analysis, identification and degrading characteristics research of the strain so as to discover the basic insecticide degrading rules of the strain and provide scientific foundation for controlling pyrethroid insecticide residues.

The invention relates to the technical field of an enzyme preparation technology, and particularly relates to a pesticide degradation enzyme and a preparation method thereof. The pesticide degradation enzyme is the supernate obtained by the broken thalli after fermentation culture of a genetic engineering strain which is collected in the China General Microbiological Culture Collection Center and the collection number of which is CGMCC No.1529. Through the adoption of the invention, the prepared pesticide degradation enzyme can degrade the organophosphorus pesticide residues and remove pesticide residues on the surfaces of fruits and vegetables to guarantee food safety.