Bacillus for degrading pyrethroid insecticides and method for producing fungicide thereof

Abstract

The invention relates to bacillus which is separated from kale phyllosphere and can degrade pyrethroid insecticides effectively, also provides a method for producing the fungicide, belonging to the biotechnology field. By identification, the bacillus is Bacillus subtilis and is preserved in CCNCC with the preservation number of CGMCC No.2698, and the 16S rDNA gene sequence of the bacillus subtilis is submitted to the GenBank database with the accession number of FJ361191. The method for producing the fungicide comprises five steps of preparing culture medium, activating strains, preparing liquid seeds, fermenting in a liquid state and preparing the fungicide. The pyrethroid insecticide degradation fungicide produced by the technical scheme of the invention can effectively degrade the pyrethroid insecticides on plant leave surfaces, reduce insecticide residual, ensure food safety and protect ecological environment.

Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a bacillus isolated from the phyllosphere of cabbage and capable of efficiently degrading pyrethroid pesticides, and a production method thereof. technical background

[0002] Pyrethroid pesticides are broad-spectrum insecticides with quick-acting, high-efficiency, low toxicity, low residue, and safety to crops. They can effectively control pests in grain and oil crops and cash crops. In recent years, its use has been increasing, and pyrethroids have become one of the substitutes for traditional organophosphorus pesticides. However, the high residues of pyrethroids on tea, vegetables and other economic crops have brought Food safety issues. In recent years, it has been found that it also has great harm to the aquatic ecosystem, such as Wang Yanmei et al. (The acute toxic effect of lambda-cyhalothrin on eels, Anhui Agricultural Sciences, 2008, 36(12): 4999-5000) reported 0.0552...