337 results about "Pyrethroid pesticides" patented technology

The invention discloses a method which can carry out qualitative screening to 242 compounds (drugs or toxicants) simultaneously. A mode of liquid chromatogram-mass spectrometry (LC-MS/MS) multi-reaction monitoring (MRM) is adopted to carry out determinedness to objects by two pairs of parent ion-daughter ion pair and retention time. The 242 drugs or toxicants comprise toxic products such as opioids, amphetamine and cocaines and the like, bromazepam such as benzodiazepines and barbiturates, and common drugs, alkaloid, pesticide (including organophosphates, carbamates, pyrethroid pesticide residues and organochlorine pesticide), weed killer, raticide and the like.

The invention discloses a method for simultaneously measuring multiple residues of organic chloride and pyrethroid pesticides. The method comprises the following working procedures: crushing a tobacco sample until grain diameters are less than 450 mu m; adding 10 to 20ml of extracting solution and internal standard solution into every program of tobacco sample; extracting the mixed solution by using ultrasonic wave; filtering the mixed solution by using a funnel filled with anhydrous sodium sulfate to obtain the extracting solution; adding the extracting solution into a Florisil solid extraction column for extracting and eluting; collecting an eluent; analyzing the eluent by using a gas chromatograph, wherein the analysis comprises the following conditions: adopting an Elite 5MS capillary column, high-purity N2 as a carrier gas, a constant flow mode in which the flow rate is 0.5 to 1.5 ml/min, a sample inlet temperature of between 280 and 320 DEG C, a detector temperature of between 330 and 370 DEG C, splitless sampling in which the sampling volume is between 1 and 2 mu L, and an initial temperature of 100 DEG C which rises from 100 to 180 DEG C at a speed of 8 DEG C/min, is kept for 4 minutes, rises from 180 to 270 DEG C at the speed of 5 DEG C/min and is kept for 15 minutes until all sample flows out completely; and determining the nature of the sample by using the retaining time of a standard sample and quantifying by using an internal standard method. In the method, ultrasonic wave is adopted for extracting and a solid extraction column is adopted for purifying, so that the method has the advantages of operating step simplification, high work efficiency, good purification effect, capability of simultaneously measuring multiple residues of 25 kinds of pesticides including 17 organic chloride pesticides of hexachlorocyclohexane, DDT and the like, seven pyrethroid pesticides such as fenpropathrin and the like, and one plant growth regulator.

The invention discloses a method for detecting pyrethroid pesticide residue amount in smoke by a cigarette filter, belonging to the technical field of tobacco chemical analysis. The method comprises the following steps: placing a sucked filter tip at standard smoking condition into a 150ml of bottle with a tapered plug; adding 100mL of mixed solvent of ethyl acetate and cyclohexane with the volume ratio of 1:1; placing the bottle on an ultrasonic wave generator for extraction for 30min; concentrating to be 0.5mL on a rotary evaporator after drying by anhydrous sodium sulfate; setting the volume to be 2mL by normal hexane to be purified; placing a small graphite carbon black column on a solid phase extractor; activating by 3mL of acetone and 3mL of normal hexane in sequence; transforming the sample test solution to be purified into the small graphite carbon black column; eluting for two times by utilizing 2.5mL of mixed solvent of acetone and normal hexane, wherein the speed does not exceed 5.0mL/min; evaporating and concentrating eluent; setting the volume to be 1.5mL by acetone and normal hexane with the volume ratio of 2:8; and analyzing on GC-ECD. The method of the invention is simple and accurate, and can effectively determine pyrethroid pesticide residue amount in smoke by the cigarette filter.

The invention discloses a mothproof fabric and a preparation method thereof. The preparation method of the mothproof fabric comprises the following steps: weaving natural fibers into an inner layer, synthetic multifilament yarn into a surface course, and polypropylene fiber filament or polyethylene fiber filament fabric into a lapping layer, wherein the inner layer and the surface course are in grid shape; dying in dying liquid at a temperature of 60-105 DEG C for 10-120 minutes; washing with water; drying; and forming, wherein the dying liquid is a missible-oil aqueous solution, missible oil is formed by mixing pyrethroid and ether solvent in a mass ratio of 1:5 to 1:20; the mass ratio of the fabric to the dying liquid is 1:7 to 1:20; and the amount of the missible oil is 5-10% of the fabric weight. The method has short production flows, convenient operation, small consumption of a pyrethroid pesticide and low requirements for fiber material by adopting dying and the additional pyrethroid pesticide; and the fabric prepared by the method has favorable permeability, strong capability of resisting insect invasion and long anti-mosquito effect.

The invention relates to a pesticide-fertilizer composition containing nereistoxin and pyrethroid pesticides. The pesticide-fertilizer composition comprises active ingredients, efficiency enhancing ingredients and fertilizer ingredients, wherein the active ingredients consist of nereistoxin pesticides and pyrethroid pesticides, the efficiency enhancing ingredients consist of fulvic acid and silicon abundance ring, and the fertilizer ingredients comprise any one or more than one of humic acid, amino acid, ammonium sulfate, potassium sulphate, urea, ammonium nitrate, ammonium bicarbonate, potassium chloride, monopotassium phosphate, calcium superphosphate and calcium-magnesia phosphate fertilizer. By application of the drug fertilizer composition, the yield of crops can be improved, the prevention effect on injurious insects can be improved, and the pesticide residue can be reduced. The weight ratio of the nereistoxin pesticides to the pyrethroid pesticide is 10-50: 1-5.

The invention discloses a detection method of pyrethroid pesticide remained in rice. The method comprises the following steps of: fully extracting pyrethroid pesticide with acetonitrile for 4 h; vibrating with ultra-pure water and then standing still for 30 min so that the pyrethroid pesticide is separated very well; centrifugally splitting the phase of the pyrethroid pesticide for 6 min to have better split phases and higher extraction rate; leaching and cleaning with hexane and acetone (in a ratio of 90 to 10) to better remove the pyrethroid pesticide; and increasing a column temperature to 260 DEG C at a programmed temperature rise rate of 40 DEG C/min and keeping both a sampling temperature and a detector temperature at 260 DEG C. The gas chromatograph is simple and convenient to operate, finishes the separation and the detection of the pyrethroid pesticide in a shorter time and has simple and rapid gas chromatography conditions and more accurate measuring value. Accordingly, the detection method of the pyrethroid pesticide remained in rice has higher extraction rate, simple and rapid gas chromatography conditions, lower operation requirement and higher quantitative recovery rate.

The invention provides an analyzing method for simultaneously determining residues of organophosphorus pesticides and pyrethriods pesticides in soil. The analyzing method is characterized by comprising the following steps: carrying out ultrasonic extraction on a soil test sample by using an acetonitrile solution containing 1% of acetic acid by volume percent; purifying by using a suitable amount of PSA and C18 mixed fillers; blowing liquid supernatant by nitrogen and concentrating until the liquid supernatant is nearly dried; respectively dissolving by acetone and n-hexane and enabling the volume to be constant; raising the temperature by using DB-1701 and HP-5 quartz capillary columns and separating; carrying out GC/FPD (Gas Chromatograph/Flame Photometric Detector) detection and GC/micro ECD (Electron Capture Detector) detection; and finally, quantifying by using a base material external standard method. A test confirms that the method has a wide linear range; the technical indexes including detection sensitivity, accuracy and precision meet the requirements of a residue analysis; the pre-treatment operation is simple, convenient and rapid and a reliable manner is provided for analytical researches for simultaneously determining the residues of ths pesticides and the pyrethriods pesticides in the soil.e organophosphoru.

The invention discloses a quantitative detection method for pyrethriods pesticides in fresh tea. The quantitative detection method is characterized by comprising the following steps: ultrasonically extracting a fresh tea sample by an acetonitrile solution containing 1% of acetic acid in percentage by volume, then purifying by using an appropriate amount of mixed filler of PSA (Prostate Specific Antigen), C18 and GCB, carrying out nitrogen blowing to concentrate liquid supernatant to be nearly dry, dissolving with normal hexane and making up to volume, increasing programmed temperature and separating by using an HP-5 quartz capillary column, carrying out GC/muECD, and finally quantifying by using a matrix external standard method. Through confirmation by test, the method is wide in linear range; the technical indexes such as detection sensitivity, accuracy and precision meet the requirements of analysis of residues; the pretreatment operation is simple, convenient and quick; through the method, a reliable manner is provided for analysis and research for detecting the residues of the pyrethriods pesticides in the fresh tea.

A disclosed preparation method for a beta-cyclodextrin chiral stationary phase comprises: taking SiO2 microballoons as a raw material, and bonding beta-cyclodextrin (beta-CD) to the surface of SiO2 microballoons by employing 3-aminopropyltriethoxysilane (KH550), trimethylchlorosilane and 4,4'-methylenediphenyl diisocyanate (MDI) to obtain the beta-cyclodextrin chiral stationary phase. The method has continuity, simple operation and short synthetic period, and the synthetic product is convenient to be subjected to derivatization; no metal ion-containing catalysts such as NaOH, NaN3, CuI(PPh3) and the like are introduced according to the method, so that the chiral stationary phase is prevented from pollution, and the method relatively accords with the separation requirement of high-purity liquid chromatogram. The method is applicable to separation of benzene ring-containing chiral medicament intermediates and pyrethroid pesticide compounds.

The invention discloses a rapid detection method of synthetic pyrethroid pesticide residual, which comprises the following steps: (1) extraction the detected material: putting the synthetic pyrethroid pesticide residual in the reactor; (2) decomposition, gas production reaction: decomposing the detected material into compound within cyanide; adding the solid particle of sodium borohydride and adsorption host to mix with the detected material; adding sulphuric acid solution in the reactor to react large amount of gas; (3) colour reaction: guiding the decomposed gas in the absorber; reacting with the specific developer to make solution display blue; (4) qualitative analysis: generating qualitative or semiquanlitative analysis of synthetic pyrethroid pesticide in comparison with the colorimetric card.

The invention discloses a method for removing pesticide residues in a natural extract. The method comprises the following steps of adding a liquid natural extract containing the pesticide residues or a liquid natural extract mixed with edible oil into an extraction kettle; causing irregular random metal packing to be arranged in the extraction kettle; and extracting the pesticide residues in the liquid natural extract with a static and dynamic combined supercritical CO2 fluid extraction method, and then resolving a CO2 solvent to finally obtain the natural extract without the pesticide residues. According to the method, any organic solvent and toxic and harmful substances are not used as a remover. The method has the characteristics that the removing process is safe, and no secondary pollution exists. According to the method, the irregular random metal packing is added, so that the removing effect of the pesticide residues is effectively increased, and the phenomena of bias current, channeling and the like are avoided. The method has the characteristics of higher removal rate, simple and easy process and low production cost and is particularly suitable for removing organophosphorus, carbamates and pyrethroid pesticide residues in partial-fat soluble natural extracts.

The invention relates to a pesticide-fertilizer composition containing Fipronil and pyrethroid pesticides, which comprises an active component, an synergistic component and a fertilizer component, wherein the active component is composed of Fipronil and pyrethroid pesticides; the synergistic component is composed of fulvic acid and Guifenghuan; and the fertilizer component comprises one or more of ammonium sulfate, potassium sulfate, urea, ammonium nitrate, ammonium bicarbonate, potassium chloride, potassium dihydrogen phosphate, calcium superphosphate and calcium magnesia phosphate fertilizer. The pesticide-fertilizer composition can increase the crop yield and enhance the prevention effect on soil pests.

The invention provides a preparation method and application of a molecular imprinted sensor with a silver nanoparticle active substrate, and belongs to the technical field of preparation of functional materials. The preparation method comprises the following steps: step one, preparing Ag nanoparticles with different morphologies; step two, preparing an Ag-MIPs molecular imprinted sensor with thesilver nanoparticle active substrate. According to the preparation method and the application disclosed by the invention, by combining a Raman detection technology with a molecular imprinting technology, a product has sensitive detectability and high selectivity. The invention relates to a novel self-assembly method: the molecular imprinted sensor with the active substrate modified by silver nanoparticles is prepared by using a small amount of acid and a novel method is provided for detecting residual pyrethroid pesticide in river water.

The invention relates to a preparation method of a yeast microsphere magnetic composite material surface imprinted adsorbent. The preparation method is characterized by taking a yeast microsphere magnetic composite material as a matrix material, pyrethroid pesticide as template molecules, alpha-methacrylic acid (MAA) as functional monomers, ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent and 2,2'-azobisisobutyronitrile (AIBN) as an initiator to prepare the yeast microsphere magnetic composite material surface imprinted adsorbent through a suspension polymerization process. The preparation method has the advantages that the template molecules are thoroughly removed, and the compatibility with an imprinted high polymer molecular layer is good. The prepared magnetic composite material surface imprinted adsorbent can be used for selectively identifying and separating pyrethroid pesticide in environmental water, and the problem that partial template molecules cannot be eluted due to too-deep embedding is avoided, and the obtained imprinted adsorbent is high in mechanical strength, identification points are not easily damaged, and nonspecific adsorption is great reduced.

The invention discloses a solid microemulsion comprising pyrethroid pesticide and a preparation method of the solid microemulsion. The solid microemulsion comprises the following components by weight percent: 3-40% of active pharmaceutical ingredients of the pyrethroid pesticide, 3-20% of surfactant, 0-10% of organic solvent, 0-6% of disintegrant, 0-3% of binder and the balance of carrier. The invention further discloses a preparation method of the solid microemulsion comprising the pyrethroid pesticide. The biological activity of active ingredients of the solid microemulsion disclosed by the invention can be fully exerted, and the shelf life of the product can be prolonged. In addition, because the solid microemulsion does not adopt polar organic solvents, aromatic hydrocarbon organic solvents such as xylene and other chemical solvents or adopts a small amount of green vegetable oil ester solvents, the pollution of the chemical solvents to the environment can be avoided.

The invention provides a bacillus licheniformis UTM104 strain for producing pyrethroid hydrolase and application of the bacillus licheniformis UTM104 strain. The biological preservation number of the bacillus licheniformis UTM104 strain is CGMCC No.9509. The UTM104 strain can be subjected to aerobic composting fermentation in organic solid wastes such as sewage sludge, household garbage, animal carcass, livestock manure and crop straws, can form a stable ecological system of a bacterial colony in an organic material, and can massively and rapidly breed, so that the organic matters in the organic solid wastes can be effectively degraded, and thus the reduction and harmless targets are rapidly achieved. Meanwhile, a microbial agent prepared by the bacillus licheniformis UTM104 strain is capable of transforming the organic solid wastes into a bio-fertilizer; and the UTM104 strain is capable of degrading pyrethroid pesticide residues on soil and plants, so that the food safety is ensured; and the strain disclosed by the invention is excellent in property, and has the advantages of relatively good social benefits and economic benefits.

The invention discloses a preparation method and application of a molecularly imprinted solid phase extraction small column for separating a pyrethroid pesticide. The molecularly imprinted solid phase extraction small column for separating the pyrethroid pesticide is characterized by consisting of cypermethrin as template molecules, methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent according to a mol ratio of 1:(2-8):10; and a pyrethroid pesticide molecularly imprinted polymer obtained with a mass polymerization method is filled into a polypropylene solid phase extraction small column to obtain the molecularly imprinted solid phase extraction small column for separating the pyrethroid pesticide. According to the invention, the pyrethroid pesticide in an aquatic product or a water environmental sample can be efficiently and selectively separated, enriched and purified. Compared with the liquid-liquid extraction and the conventional solid phase extraction, the molecularly imprinted solid phase extraction column disclosed by the invention has the advantages of high enriching and purifying efficiency, low consumption of organic solvent and the like.

The invention discloses a preparation method of high-efficiency cyhalothrin nano-pesticide, belonging to the technical field of preparation of agricultural chemicals. The preparation method is characterized in that an amphiphilic copolymer is adopted and is self-assembled in an aqueous solution to form a nano-sized micelle with an obvious core-shell structure; the nano-pesticide is obtained by physical actions of an organic solvent and polyvinyl alcohol. The preparation method disclosed by the invention is simple and convenient; raw materials are easily obtained; a used solvent is water, so the pollution is small and the cost is low; the used amphiphilic copolymer has good environmental compatibility, biodegradation and no residual risks; in addition, a degraded product can be absorbed and utilized by crops; the nano-pesticide has the advantages of relatively small and relatively uniform particle size, relatively good permeability and certain slow release effect; the lasting period of pyrethroid pesticides is prolonged, and the using dose of the pesticide is reduced.

The invention discloses a method for processing an onion sample containing organophosphorus and synthetic pyrethroid pesticide residues before determination, comprising the following steps: processing the onion in a microwave oven for 40 seconds; stirring, mixing and obtaining an even sample; then adding 30ml of acetonitrile and 10ml of distilled water; after oscillating and extracting for 30 minutes, filtering into a separating funnel of which the volume is 500ml; washing residues by 20ml of acetonitrile; filtering a washing liquid into the separating funnel; adding 8g of sodium chloride; oscillating for 1 minute; standing and layering; abandoning a lower-layer aqueous phase; taking an upper-layer organic phase, and processing the upper-layer organic phase by anhydrous sodium sulfate; washing the anhydrous sodium sulfate for several times by 20ml of acetonitrile; combining the acetonitrile phases; reducing pressure and concentrating to be dry; adding 2ml of acetone/ normal hexane (V/V, 1/1); eddying for 10 seconds; transferring to a sample introduction flasket; and determining by a gas chromatography and mass spectrometry. With the method for processing an onion sample before determination in the invention, impurity interference can be greatly lowered, and the method has high sensitivity.

The invention discloses degrading bacteria for pyrethroids insecticides. The degrading bacteria are HP-S-01 and are identified to be streptomyces (Streptomyces sp.), wherein the collection number of the strain is CCTCC M 2010096; and Genbank access number of 16S rDNA of the strain is HM016873. The invention discloses a method for preparing bactericide by using the degrading bacteria at the same time. The bactericide is low in cost, convenient and desirable in degradation effect, can directly used to reduce cypermethrin pesticide residues in crops by over 80 percent, solves the problem of excessive cypermethrin pesticide residue in the agricultural production, is suitable to be used in national fruit and vegetable production and export bases or places with a green food brand mark on a large scale, and has a great significance for promoting the production of green vegetables and green food.