Quantitative detection method for pyrethriods pesticides in fresh tea

A quantitative detection method and pyrethroid technology are applied in the field of pesticide residue detection, which can solve the problems of unstable and reliable detection results, consumption of organic solvents, and complicated operations, and achieve stable results, low detection limits, and repeatability. Good results

Active Publication Date: 2014-07-09
江苏太湖地区农业科学研究所
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Problems solved by technology

[0006] The invention provides a quantitative detection method for pyrethroid pesticides in fresh tea leaves, the purpose of which is to solve the problem of long time-consuming, cumbersome operat

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  • Quantitative detection method for pyrethriods pesticides in fresh tea
  • Quantitative detection method for pyrethriods pesticides in fresh tea
  • Quantitative detection method for pyrethriods pesticides in fresh tea

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Embodiment

[0040] Example: A Quantitative Detection Method for Pyrethroid Pesticides in Fresh Tea Leaves

[0041] The fresh leaves of the tested tea were taken from a local tea plantation in Suzhou, crushed and mixed by a pulverizer, put into a clean container, and stored in a -20°C refrigerator for later use. The quantitative detection method consists of two parts:

[0042] The first part is to establish the standard curve of the measured pyrethroid pesticides with known gradient concentration respectively with gas chromatography; the establishment of the standard curve is composed of the following steps:

[0043] The first step is to prepare a blank matrix extract, and the preparation method consists of the following steps:

[0044] (1) Take by weighing 5.0g the blank tea fresh leaf sample that does not contain tested pyrethroid pesticides, place in a 50mL centrifuge tube, then add 10mL of acetonitrile solution containing 1% acetic acid by volume fraction in the centrifuge tube, Mix ...

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Abstract

The invention discloses a quantitative detection method for pyrethriods pesticides in fresh tea. The quantitative detection method is characterized by comprising the following steps: ultrasonically extracting a fresh tea sample by an acetonitrile solution containing 1% of acetic acid in percentage by volume, then purifying by using an appropriate amount of mixed filler of PSA (Prostate Specific Antigen), C18 and GCB, carrying out nitrogen blowing to concentrate liquid supernatant to be nearly dry, dissolving with normal hexane and making up to volume, increasing programmed temperature and separating by using an HP-5 quartz capillary column, carrying out GC/muECD, and finally quantifying by using a matrix external standard method. Through confirmation by test, the method is wide in linear range; the technical indexes such as detection sensitivity, accuracy and precision meet the requirements of analysis of residues; the pretreatment operation is simple, convenient and quick; through the method, a reliable manner is provided for analysis and research for detecting the residues of the pyrethriods pesticides in the fresh tea.

Description

technical field [0001] The invention relates to the field of detection methods for pesticide residues, in particular to a quantitative detection method for pyrethroid pesticides in fresh tea leaves. The quantitative detection method is to extract and purify fresh tea leaves by using the improved QuEChERS method, and then The detection of pyrethroid pesticides by gas chromatography is fast, simple and reliable. Background technique [0002] Tea is one of the three major plant beverages in the world. It is more and more popular because of its unique taste and health effects such as anti-cancer, anti-mutation, scavenging free radicals, and delaying aging. my country is a big tea producing and exporting country. The application of pesticides is the most effective means to ensure the yield of tea and control the threat of pests and diseases during its growth. Pyrethroid pesticides (English name: Pyrethroid pesticides) are widely It is widely used in the prevention and control of ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
Inventor 董明辉刘腾飞顾俊荣张丽邓金花杨代凤陆皓茜钱辉
Owner 江苏太湖地区农业科学研究所
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