Preparation method of peony stamen and pistil protein powder

A peony stamen and protein powder technology, which is applied in the field of food processing, can solve the problems of not improving the solubility and emulsification of peony stamen protein at the same time, and being unable to expand the application range of peony stamens, etc.

Pending Publication Date: 2022-02-01
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current prior art, there is no method for simultaneously improving protein solubility and emulsification of peony stamens, and it is impossible to expand the scope of application of peony stamens

Method used

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  • Preparation method of peony stamen and pistil protein powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:

[0036] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through an 80-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:5 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.

[0037] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:20 (g / mL) to obtain a mixed solution, first add 0.1mol / L of sodium hydroxide to adjust the pH of the mixed solution to 9.0, Then heated in a water bath at 30° C. for 3 h, centrifuged at 4000 r / min for 20 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for later use.

[0038] (3) With the alkal...

Embodiment 2

[0044] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:

[0045] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through a 90-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:6.5 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.

[0046] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:25 (g / mL) to obtain a mixed solution, first add 0.25mol / L of sodium hydroxide to adjust the pH of the mixed solution to 9.3, Then heated in a water bath at 40° C. for 2 h, centrifuged at 6000 r / min for 15 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for future use.

[0047] (3) With the ...

Embodiment 3

[0053] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:

[0054] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through a 100-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:8 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.

[0055] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:30 (g / mL) to obtain a mixed solution, first add 0.5mol / L of sodium hydroxide, and then adjust the pH of the mixed solution to 9.5 , and then heated in a water bath at 50° C. for 1 h, centrifuged at 8000 r / min for 10 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for subsequent use.

[0056]...

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Abstract

The invention relates to a preparation method of peony stamen and pistil protein powder, and belongs to the technical field of food processing. The preparation method of the peony stamen and pistil protein powder comprises the following steps: carrying out protease enzymolysis treatment on extracted peony stamen and pistil protein, carrying out glycosylation treatment on an enzymolysis product, and drying to obtain the peony stamen and pistil protein powder. According to the preparation method of the peony stamen and pistil protein powder, the peony stamen and pistil protein is modified by adopting a method of combining enzymolysis treatment and glycosylation reaction, the solubility and emulsibility of the peony stamen and pistil protein powder can be improved, chemical reagents are not added, and the preparation method is green, safe, simple to operate and easy for large-scale food processing and production.

Description

technical field [0001] The invention relates to a preparation method of peony stamen protein powder, and specifically belongs to the technical field of food processing. Background technique [0002] Peony flowers are gorgeous in color and are known as the "king of flowers". Our country has the habit of eating peony flowers since ancient times, and has formed a variety of local specialties. In 2013, Danfeng Peony was listed as a new kind of food by the Ministry of Health. Resource food raw materials have been developed and utilized by many researchers. Peony stamen is the essence of peony. It has high carbohydrate and protein content and healthy fatty acid composition. It can lower human blood pressure and has good anti-oxidation effect. It plays an important role in the regulation of human physiological metabolism and dietary structure. Peony stamens are a plant seed material with the same source of medicine and food. The protein content accounts for 23.74% of the dry weigh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/107C07K1/14C07K1/30C07K1/36
CPCC12P21/06C07K1/1077C07K1/30C07K1/145C07K1/36
Inventor 罗磊夏迎利焦昆鹏马丽苹赵一帆杨浩昆李瀚姝马潇
Owner HENAN UNIV OF SCI & TECH
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