High-absorptivity compound protein composition as well as preparation method and application thereof

A compound protein and compound protease technology, which is applied in the field of high absorption compound protein composition and its preparation, can solve the problems of physical, chemical and biological function changes, unstable properties of whey protein, and restrictions on the application of whey protein. The preparation method is simple, the function is rich, and the effect of promoting metabolism

Active Publication Date: 2022-07-05
北京幸福能量健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whey protein itself is also unstable, and its high-level structure is easily destroyed during processing and productio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A preparation method of a high absorption rate composite protein composition, specifically comprising the following steps:

[0053] S1. Preparation of protein solution: after mixing 5 parts by weight of whey protein isolate powder, 3 parts by weight of concentrated milk protein powder, and 2 parts by weight of hydrolyzed whey protein powder, to obtain protein powder, add dihydrogen phosphate with a pH value of 7.2 In the sodium-citric acid buffer solution, a protein solution is obtained; the solid-liquid ratio of the protein powder and the disodium hydrogen phosphate-citric acid buffer solution is 1:50 g / mL;

[0054] S2. Activation of strains: Lactobacillus plantarum, Bacillus coagulans, and Bifidobacterium adolescentis were inoculated into sterile MRS liquid medium at 36°C under microaerobic conditions (the microaerobic condition was that the oxygen concentration was 5%, CO 2 The concentration is 3%, the balance is nitrogen, where % is the volume percentage), 20r / min, ...

Embodiment 2

[0061] A preparation method of a high absorption rate composite protein composition, specifically comprising the following steps:

[0062] S1. Preparation of protein solution: after mixing 10 parts by weight of whey protein isolate powder, 5 parts by weight of concentrated milk protein powder, and 5 parts by weight of hydrolyzed whey protein powder, obtain protein powder, add dihydrogen phosphate with a pH value of 8 In the sodium-citric acid buffer solution, a protein solution is obtained; the solid-liquid ratio of the protein powder and the disodium hydrogen phosphate-citric acid buffer solution is 1:100 g / mL;

[0063] S2. Activation of strains: Lactobacillus plantarum, Bacillus coagulans and Bifidobacterium adolescentis were inoculated into sterile MRS liquid medium at 40°C under microaerobic conditions (the microaerobic condition was that the oxygen concentration was 10%, CO 2 The concentration is 7%, and the balance is nitrogen, where % is the volume percentage), 50r / min,...

Embodiment 3

[0070] A preparation method of a high absorption rate composite protein composition, specifically comprising the following steps:

[0071] S1. Preparation of protein solution: after mixing 7 parts by weight of whey protein isolate powder, 4 parts by weight of concentrated milk protein powder, and 3.5 parts by weight of hydrolyzed whey protein powder, protein powder was obtained, and dihydrogen phosphate with a pH value of 7.7 was added. In the sodium-citric acid buffer solution, a protein solution is obtained; the solid-to-liquid ratio of the protein powder and the disodium hydrogen phosphate-citric acid buffer solution is 1:70 g / mL;

[0072] S2. Activation of strains: Lactobacillus plantarum, Bacillus coagulans, and Bifidobacterium adolescentis were inoculated into sterile MRS liquid medium at 37°C under microaerobic conditions (the microaerobic condition was that the oxygen concentration was 7%, CO 2 The concentration is 5%, the balance is nitrogen, where % is the volume per...

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Abstract

The invention provides a high-absorptivity compound protein composition as well as a preparation method and application thereof, and belongs to the technical field of protein. The preparation method comprises the following steps: adding whey protein powder into a disodium hydrogen phosphate-citric acid buffer solution, inoculating lactobacillus plantarum, bacillus coagulans and bifidobacterium adolescentis for fermentation, adding compound protease into fermentation liquor for enzymolysis to obtain enzyme hydrolysate, further performing synchronous ultrasonic wave and ozone treatment, performing enzymolysis through cross-linked compound enzyme, and performing freeze-drying to obtain the whey protein powder. And adding a metal ion solution, heating, stirring and reacting to prepare the high-absorptivity composite protein composition. The high-absorptivity composite protein composition prepared by the invention is moderate in molecular weight, does not cause a first-pass effect of liver, has high absorption and utilization rate, is rich in protein function, and has obvious effects of resisting aging, resisting oxidation, promoting fat burning and promoting metabolism.

Description

technical field [0001] The invention relates to the technical field of proteins, in particular to a high absorption rate composite protein composition and a preparation method and application thereof. Background technique [0002] Aerobic organisms, including humans, take in oxygen through breathing in order to survive. At this time, part of the oxygen taken into the body is converted into reactive oxygen species such as peroxide radicals, hydrogen peroxide, and hydroxyl radicals during energy metabolism. . Such reactive oxygen species are originally involved in biological defense, including the pathogen removal mechanism of macrophages during bacterial or viral infection, and play an important role in maintaining health. However, due to environmental factors such as air pollution, ultraviolet rays, lifestyle habits such as smoking, mental stress, etc., the balance in the living body is disrupted, and once the reactive oxygen species is excessive, it will react with protein...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P21/06C12P21/00C07K1/107C12N1/20A23L33/18A23L33/165C12R1/25C12R1/07C12R1/01
CPCC12P39/00C12P21/06C12P21/00C07K1/1077C12N1/20A23L33/18A23L33/165A23V2002/00A23V2250/55A23V2250/1588A23V2250/1642A23V2250/1592A23V2200/302A23V2200/30Y02A40/90
Inventor 张光明董洪波赵杰王颖张琦峰万宏颖洪伟雄
Owner 北京幸福能量健康科技有限公司
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