1results about How to "Solve degradation" patented technology

The application provides a repeated protein expression method and integrated device using anti-degradation circular DNA and circular RNA. The repeated protein expression method comprises a DNA template capable of producing circRNA in a cell-free protein expression system; the DNA template is a double-stranded DNA template with closed ends formed by two single-stranded DNAs; the double-stranded DNA template is connected with a closed linker at two ends of a linear DNA template; the closed linker comprises a 5' end linker and a 3' end linker; the sequence of the non-coding region of the 5' end linker and the sequence of the non-coding region of the 3' end linker can be transcribed into a cleavage site of a circRNA precursor, and a circRNA is formed through a sequence complementary affinity effect. According to the technical scheme of the application, the circRNA can be continuously produced in the cell-free protein expression, the degradation of the DNA fragment is solved, the protein expression efficiency is improved, and the template can be repeatedly used after the hydrogel is covalently bonded with the template, so that the production cost is further reduced.