2results about How to "Solve degradation" patented technology

The invention provides a recombinant IL-36Ra food-grade lactococcus lactis expression plasmid as well as a preparation method and application thereof. The expression plasmid comprises a base fragment for coding the interleukin-36 receptor antagonist; the expression plasmid can express IL-36Ra with multiplied activity, and the expression plasmid is a food-grade intracellular expression vector of lactococcus lactis and does not have antibiotic genes. Compared with traditional IL-36Ra protein injection and plasmid injection, the food-grade lactococcus lactis expression plasmid is simple to operate and relatively low in cost, and a new thought is provided for research on in-vivo gene therapy and IL-36Ra gene functions.

The application provides a repeated protein expression method and integrated device using anti-degradation circular DNA and circular RNA. The repeated protein expression method comprises a DNA template capable of producing circRNA in a cell-free protein expression system; the DNA template is a double-stranded DNA template with closed ends formed by two single-stranded DNAs; the double-stranded DNA template is connected with a closed linker at two ends of a linear DNA template; the closed linker comprises a 5' end linker and a 3' end linker; the sequence of the non-coding region of the 5' end linker and the sequence of the non-coding region of the 3' end linker can be transcribed into a cleavage site of a circRNA precursor, and a circRNA is formed through a sequence complementary affinity effect. According to the technical scheme of the application, the circRNA can be continuously produced in the cell-free protein expression, the degradation of the DNA fragment is solved, the protein expression efficiency is improved, and the template can be repeatedly used after the hydrogel is covalently bonded with the template, so that the production cost is further reduced.