51results about How to "Solve degradation" patented technology

The invention discloses pyrethroid pesticide residue degradation bacteria as well as a bacteria agent and an application. A strain is Bacillussp. DG-02 with the strain preservation number of CCTCCM2011382. The degradation bacterial agent prepared by applying the Bacillussp. DG-02 is low in production cost, convenient to use and good in degradation effect, can be directly used to reduce the residual amount of deltamethrin, fenpropathrin, lambda-clyhalothrin, beta-cypermethrin, beta-cyfluthrin and 3-phenoxybenzoic acid in soil and crops by more than 75%, the problem of overproof pesticide residue in agricultural production is solved, and the degradation bacterial agent is suitable for being used in a large scale in fruit and vegetable production export bases across the country or places with green food brand marks, and has great significance in promoting the production of nuisance-free agricultural products and green food.

The invention discloses a recombinant human III-type collagen and a pichia pastoris recombinant expression system thereof. According to the invention, a Gly-X-Y structural peptide containing valine, phenylalanine, leucine, methionine and alanine in a part of peptide fragments of human III-type collagen is replaced with a Gly-X-Y structural peptide which is high in use frequency and does not contain the amino acid, and a gene sequence for encoding the peptide fragments is obtained according to the peptide fragments after the replacement. And then gene expression vectors for tandem expression ofpeptide fragments with different repeat numbers are constructed and transformed into pichia pastoris host bacteria, and a strain for high expression of the recombinant human III-type collagen is obtained through screening. Through artificial design optimization of a protein structure, the problem that natural collagen is degraded in fermentation is effectively solved, and an expression product has high stability and good biocompatibility and can be used for large-scale production.

The invention relates to a clopidogrel bisulfate tablet. A preparation method of the clopidogrel bisulfate tablet comprises the following steps of mixing clopidogrel bisulfate, lactose and hydroxy propyl cellulose according to a weight ratio of 1: (0.02 to 1): (1 to 3), preparing the mixture into pellets, dressing the pellets, and pressing the dressed pellets and an appropriate amount of magnesium stearate into tablets. Compared with the prior art, the preparation method adopts a pellet dressing and tabletting technology, improves product stability and curative effects, and prevents the sticking problem produced in drug tabletting.

This invention relates to gene cloning and gene expression, more specially, a method for expressing recombinant hypoglycemic peptide with long-lasting effects. The method comprises: mutating the digestion site of dipeptidyl peptidase in GLP-1 from alanine to glycine, ligating with human albumin gene, and expressing in Pichia expression system to obtain four fusion proteins, which comprise recombinant GLP-1, human albumin and flexible linkers. The fusion proteins have such advantage as retained GLP-1 activity, long half-life period, good hypoglycemic effect, and no hypoglycemia phenomenon.

The invention discloses a rumen bypass GABA cow concentrate which comprises a base material and a coated gamma-aminobutyric acid; the base material comprises the following raw materials on a dry matter weight basis: 52-54 parts of corns, 10-12 parts of soybean meal, 6-8 parts of cottonseed cake, 4-6 parts of cottonseed meal, 7-9 parts of sesame meal, 7-9 parts of wheat bran, 1.5-2.5 parts of calcium hydrogen phosphate, 1.5-2.5 parts of light calcium carbonate, 0.5-1.5 parts of calcium bicarbonate, 0.5-1.5 parts of edible salt, and 0.5-1.5 parts of sodium bicarbonate; the weight ratio of the coated gamma-aminobutyric acid and the base material dry matter is 75-245 mg / kg. The invention can increase the feed intake and reproduction performance of cows at a lactation peak period.

The invention relates to a vertical type animal gastrointestinal bionic digester, which comprises a motor, a connecting shaft sleeve, a heightening transmission rod, a stirring rod and a simulated digestive tract, wherein the connecting shaft sleeve is sleeved on an output shaft of the motor and synchronously rotates along with the motor, and the top end of the heightening transmission rod is fixedly sleeved in a sliding chute below the connecting shaft sleeve by a screw rod, so that the heightening transmission rod can move up and down in the connecting shaft sleeve; a locking nut is arranged on the heightening transmission rod and is used for adjusting the locking degree of the heightening transmission rod and the stirring rod, and the simulated digestive tract is a double-layer hollow vertical glass tube; the stirring rod is arranged in an accommodating cavity of the simulated digestive tract, and the top end of the stirring rod penetrates through a sealing plug at the top of the simulated digestive tract and is connected with the bottom end of the heightening transmission rod. The vertical type animal gastrointestinal bionic digester provided by the invention has the advantages of simple structure, convenience in use, avoidance of leakage of a bionic digestion reaction liquid, high accuracy and good repeatability of digestion simulation, and small errors; the degradation difficulty of cellulose on a dialysis bag in the existing simulated digestive tract in a bionic digestion reaction is effectively solved.

Belonging to the field of biomedical engineering, the invention specifically related to a silk artificial blood vessel and a preparation method thereof. The artificial blood vessel includes a silk woven blood vessel body and glycosylated cross-linking sericin filling blood vessel body fiber gaps. The preparation method includes the steps of: preparation of the blood vessel body, compounding of the blood vessel body and glycosylated sericin, blood vessel surface modification and endothelial pre-coating. In the invention, glycosylated sericin is compounded with silk, then aldehyde is employed to crosslink sericin so as to reduce water solubility, the gaps between artificial blood vessel silk fiber are filled to prevent blood leakage, and the rigidity of the blood vessel is improved. The glycosylated sericin is located on the artificial blood vessel surface, can reduce immunogenicity and improve the anticoagulation effect, and makes the artificial blood vessel difficult to degrade, thus overcoming the two defects of easy degradation and strong immunogenicity in the prior art. And the silk artificial blood vessel has the advantages of simplicity and practicability, good quality and low price.

The invention relates to the field of application of B lymphocyte stimulating factor acceptor-antibody fusion protein to treatment of autoimmunity diseases, in particular to an application of TACI-Fcfusion protein to preparation of medicines for treating neuromyelitis opticaspectrum disorders (NOOSD) and multiple sclerosis (MS).

The invention discloses separation of sihydroartemisinic acid from artemisinin production waste by means of alkali dissolution and acid deposition. The method comprises the following steps of: concentrating in vacuum to volatilize residual solvents in production waste liquid or waste paste by using the artemisinin production waste liquid or the waste paste as raw materials; ultrasonically extracting by using alkali solution; mixing extracting solution with one another and removing sediments to obtain supernate; adding acid solution into the supernate; collecting sediments; washing the sediments by using clean distilled water; freeze-drying to obtain a dry sihydroartemisinic product. A method for separating sihydroartemisinic from the artemisinin production waste is established at first, and then an ultrasonic-assisted extracting method is adopted, so that the extraction efficiency and the recovery rate are effectively improved; by utilizing a freeze-drying method, the problem of degradation of the sihydroartemisinic acid product caused in the traditional drying method is effectively solved; the purity of the product is more than 72 percent, and the recovery rate is more than 90 percent; moreover, the production capacity is high, and the industrialized production is easily realized.

The invention discloses an absorbent used for removing acid gas and having oxidative degradation resistant performance. The absorbent contains organic amine, and alcoholic and phenolic antioxygen. During removal of acid gas, the absorbent can form an organic phase and a carrier phase. The phenolic antioxygen can be dissolved in high-carbon alcoholic carrier phases to play an oxidative degradation resistant role, and also can be dissolved in an organic phase to play a thermal degradation resistant role. The phenolic antioxygen is a tree-like macromolecule phenolic antioxygen obtained by taking a compound containing a phenolic group as an initiator and introducing the phenolic group to the surface of polyamide-amine branch-shaped macromolecules.

The invention belongs to the field of biodegradable plastics, and discloses a method for preparing attapulgite-based biodegradable plastics from a natural attapulgite-loaded biopolymer material and a photocatalytic material. Natural attapulgite is subjected to functionalization treatment to serve as a carrier, a biological polymer material and a photocatalytic material are adsorbed through a physical / chemical method, modified attapulgite composite powder is prepared, the modified attapulgite composite powder and biodegradable plastic powder are blended to prepare a modified master batch, and the attapulgite-based biodegradable plastic which is low in cost, good in mechanical property and high in degradation rate is prepared through processing and forming. The attapulgite-based biodegradable plastic prepared by the method has the characteristics of good mechanical property, low cost, high biological decomposition rate and the like, can be applied to replace traditional non-degradable plastic, and provides a new way for solving the problem of white pollution.

The invention relates to the field of construction additives, and in particular relates to a conditioning agent for residue soil. The conditioning agent for the residue soil comprises the following raw materials: a solid material and a liquid material; and the solid material comprises the following components, in percentages by weight, 1.4%-18.0% of polyacrylamide, 5.7%-72.1% of nonionic celluloseether, 9%-21.4% of an inorganic base, and 0.9%-71.4% of a sulfonic acid surfactant. The conditioning agent provided by the invention solves the problems of fluidity, soil discharge and degradation bymicroorganisms in soil of a sandy gravel stratum containing relatively large particles.

The invention relates to the maritime aerial image defogging field and discloses an adaptive weight dark channel defogging algorithm for maritime aerial images of an unmanned aerial vehicle. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle fully considers the maritime aerial image defogging specificity, to be specific, the adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle does not only consider the effective defogging for a maritime specific object but also considers the fast, effective and correct defogging for a sea surface region in an image. The adaptive weight dark channel defogging algorithm combines foggy image quality degradation features with specific features of the sea area in the maritime aerial image, abandons a traditional defogging method, and fully considers the specificity of the sea area in the maritime foggy image. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle mainly includes that acquiring maritime aerial images of the unmanned aerial vehicle, performing dark channel defogging algorithm, performing adaptive weight algorithm, and defogging the maritime aerial images of the unmanned aerial vehicle. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle realizes the fast defogging for the maritime aerial images of the unmanned aerial vehicle and has broad prospect in the maritime or island aerial monitoring aspect.

The invention provides a VOCs waste gas treatment method combining absorption and adsorption. The method includes the following steps: absorbing VOCs exhaust gas in a first absorption tower under normal temperature and normal pressure, extracting non-standard gas from the top of a tower, introducing the non-standard gas into a rotating wheel for concentration or other adsorption systems, discharging purified gas reaching the standard directly, discharging concentrated gas with VOCs into a second absorption tower after residual VOCs in the non-standard gas is concentrated by absorption-adsorption, discharging the gas absorbed and purified in the second absorption tower after the gas reaches the standard, combining a first absorbent pregnant solution of the first absorption tower and a second absorbent pregnant solution of the second absorption tower, exchanging heat between the combination and the regenerated lean liquid extracted from a desorbing tower, then introducing the combinationinto the desorbing tower after overheating for steam stripping and regeneration in the tower. The treatment method is suitable for exhaust gas in low, medium and high concentration, and has remarkable effects on treatment of the exhaust gas with VOCs concentration below 5000 mg / m3.

The invention discloses an extraction and detection method of rapeseed glucosinolate, which belongs to the field of deep processing of oil crops. The extraction and purification method of glucosinolate in rapeseeds is improved through comprehensive application of technologies such as constant-temperature water bath, ultrasonic extraction, a solid-phase extraction column, a microfiltration membraneand the like; an ion pair extraction method is utilized to establish an accurate and efficient rapeseed glucosinolate analysis method; the method can meet the detection requirements of 14 known rapeseed glucosinolates, and six newly discovered glucosinolate components existing in rapeseeds are extracted and qualitatively characterized for the first time. The method solves the problem of unclear degradation mechanism of the traditional rapeseed glucosinolate as a flavor precursor substance, improves the stability of the rapeseed glucosinolate in the extraction process, and has great application potential and economic value in the fields of rapeseed oil processing raw material selection, breeding direction and the like.

The invention discloses a method for extracting total RNA (Ribonucleic Acid) of grapholita molesta adult antennae. The method comprises the following steps of: freezing grapholita molesta adults for coma under a low temperature condition, shearing a plurality of antennae by using small medical scissors and forceps, immediately soaking the antennae in a 1.5ml centrifugal tube in liquid nitrogen, taking the centrifugal tube out of liquid ammonia, immediately adding a small amount of RNA extraction liquid, fully homogenizing the extraction liquid by using a homogenizer, adding enough total RNA extraction liquid, blowing for a few times by use of a pipettor, transferring to the other new centrifugal tube, performing chloroform extraction according to a total RNA extraction method, and performing isopropanol and ethanol precipitation and separation, thus obtaining high-purity total RNA. The method is simple and convenient, and can be used for solving the problem about degradation of the RNA in the extraction process, so that the high-quality total RNA is obtained.

The invention discloses a decomposing method of viscous oil effluent chemical oxygen demand, which is characterized by the following: fetching water from anaerobic pond; adding H2O2 solution and FeSO4 solution sequently; blending to react with effluent; making effluent not generate toxic material; dissolving water in the oil acid, alcohol and water; depositing after 2h; forming transparent non-odor oil chemical oxygen demand water to satisfy draining standard.

The invention discloses a cyclodextrin-Auricularia auricula micropowder combination which is mainly prepared from cyclodextrin and Auricularia auricula. The invention further discloses a preparation method of the combination, a preparation comprising the combination and a preparation method of the preparation. Compared with the prior art, the cyclodextrin-Auricularia auricula micropowder combination and the preparation have the advantages that release of active ingredients can be promoted, water solubility of the active ingredients can be improved, and protein ingredient stability of Auricularia auricular, dispersity and performance of the preparation can be improved.

The invention relates to the field of medicine, in particular to a recombinant coxsackie virus A10VLP and application thereof, and the recombinant coxsackie virus A10VLP is generated by a cell line with capsid proteins VP2, VP3 and VP1 for coding the coxsackie virus A10 integrated in a genome, or is generated by a cell line with capsid proteins VP4, VP2, VP3 and VP1 for coding the coxsackie virus A10 integrated in a genome. The invention also provides an application of the recombinant coxsackie virus A10VLP in preparation of a product for preventing hand-foot-and-mouth disease. The product for preventing the hand-foot-and-mouth disease is a pharmaceutical composition, such as a vaccine composition. Immunogenicity research finds that the coxsackie virus A10VLP disclosed by the invention can avoid the degradation problem of VP0 and can induce good immunoreaction in a mouse body, so that the VLP can be used as a candidate vaccine of the coxsackie virus A10.

The present invention relates to a preparation method of high-molecular polyacrylamide for treating paper-making black liquor. Said preparation method includes the following steps: (1), adding soda into acrylamide aqueous solution whose concentration is 21.0%-25.0% and regulating temperature to 5-12 deg.C; (2), introducing nitrogen gas and adding chemicals and initiator, making polymerization reaction, when the temperature is risen to about 70-90 deg.C, introducing steam to make hydrolysis, after 1-4 hours of hydrolysis forming colloidal block; and (3), using granulating machine to make granulation, drying, and adding stabilizing agent and tack-producing agent, grinding and screening so as to obtain the invented final product.

The invention discloses a recombinant bacteria capable of efficiently expressing glucagon-like peptide 1 (GLP-1) or an analogue thereof. The recombinant bacteria comprise vectors for expressing the GLP-1 or the analogue thereof and vectors for interfering protease expression in the recombinant bacteria. The vectors for interfering protease expression in the recombinant bacteria are expression vectors for interfering protease expression of ClpA, ClpP, ClpQ, ClpX, ClpY, Dcp, PepD and HflB, which are designed by an RNA (Ribonucleic Acid) interference method. Expression of protease in recombinant bacteria is inhibited and interfered, the problem that the GLP-1 analogue is degraded by protease of host bacteria in the fermentation process can be effectively solved, meanwhile, hydrophilic amino acid is added during construction, protein solubility can be remarkably improved, inclusion bodies are reduced, and the proportion of a soluble part is 80%, so that the expression quantity of the GLP-1 analogue is improved to be more than 2g / L. The recombinant bacteria provided by the invention can efficiently express the GLP-1 analogue fusion protein so as to efficiently prepare the GLP-1 analogue.

The invention discloses a recombinant III-type humanized collagen, a nucleic acid, a carrier and an implant, and relates to the technical field of biological genes. Sequence peptide fragments 549-560aa, 597-613aa, 881-902aa and 648-662aa which are obtained by screening a natural human III type collagen alpha1 chain for a long time and have good stability are selected, the four peptide fragments are spliced in sequence to form monomers, and then tandem expression is performed on the monomers. Through fermentation and purification, the obtained recombinant III-type humanized collagen is high in stability, the problem of degradation of the recombinant III-type humanized collagen in the clinical use process is effectively solved, and the recombinant III-type humanized collagen can be prepared into a liquid preparation for preservation and use.

The invention relates to the field of bioengineering and specifically relates to a new process for extracting heparin by degrading lung tissues. The new process comprises the steps of mincing raw materials pig lung and bovine lung removed from fat and bronchia, preparing a compound enzyme agent and adding a coenzyme component to obtain an activating enzyme working solution, and performing enzymatic hydrolysis on the pig lung and the bovine lung by use of the activating enzyme working solution. The tissues of the pig lung and the bovine lung can be digested into water-soluble components. As the degradation conditions are mild, the active ingredients in the pig lung and the bovine lung are remained completely so that the ingredients such as heparin, protein and polypeptides can be separated conveniently.

The invention discloses a method used for processing methyl orange dye waste water with bean product waste water and waste residue. According to the method, methyl orange dye waste water is treated with waste water and waste residue generated in bean product processing process, so that methyl orange dye waste water degradation is realized, and a part of toxic harmful substances generated by secondary oxidation decomposition of bean product waste water is reduced. Soybean contains a large amount of unsaturated fatty acids including oleic acid, linoleic acid, and linolenic acid, in processing process, oxidation reaction of those unsaturated fatty acids under lipoxygenase enzyme catalytic oxidation and thermopolymerization conditions is easily caused, free radicals and hydroperoxides with high reaction capacity, and secondary oxidation products such as aldehyde and ketone generated through decomposition of the hydroperoxides are generated, and enter into bean product waste water and wasteresidue, oxidation degradation of methyl orange solution is realized, decolouring effect is achieved, and the application prospect in the field of waste water processing in environment protection ispromising.

The invention discloses skin lotion having effect of resisting oxidation. The skin lotion comprises the raw materials in percentage by weight of 5-9% of sweet almond oil, 7-14% of octadecanol, 4-7% ofsimethicone, 2-6% of caprylic triglyceride, 1-4% of a lycopene antioxidant, 3-5% of hydrolyzed protein, 10-15% of glycerine, 0.1-0.5% of ethylenediaminetetraacetic acid disodium salt and the balancedeionized water. The formula is scientific in components and compounding ratio, the prepared skin lotion is uniform in texture, good in using skin feeling, mild, and non-irritating; and the high-hydrophilicity lycopene antioxidant is added and is obtained by esterifying hyaluronic acid and lycopene intermediate acid, and the problems that the lycopene intermediate is poor in water solubility and difficult to absorb, are solved. The lycopene intermediate has high antioxidation, so that the problem that hyaluronic acid is degraded when being in contact with free radicals can be solved, the antioxidant can eliminate surplus free radicals when entering skin and can quench single oxygen, the skin can durably maintain plump and flexible, and wrinkles are avoided.

The invention discloses a recombinant bacterium for highly expressing glucagon-like peptide 1 (GLP-1) or its analogues. The recombinant bacterium of the present invention includes a vector expressing GLP‑1 or its analogue and a vector interfering with protease expression in the recombinant bacterium. The carrier that interferes with the protease expression in the recombinant bacteria is an expression vector that interferes with the expression of ClpA, ClpP, ClpQ, ClpX, ClpY, Dcp, PepD, and HflB proteases designed by the method of RNA interference. The present invention suppresses and interferes with the expression of protease in recombinant bacteria, and can effectively solve the problem of GLP-1 analogues being degraded by protease of host bacteria during the fermentation process. At the same time, adding hydrophilic amino acids during construction can significantly increase protein solubility and reduce inclusion The soluble part accounts for 80%, thereby increasing the expression level of GLP-1 analogs to more than 2g / L. The recombinant bacterium of the present invention can efficiently express the GLP-1 analog fusion protein, and further efficiently prepare the GLP-1 analog.

This invention relates to gene cloning and gene expression, more specially, a method for expressing recombinant hypoglycemic peptide with long-lasting effects. The method comprises: mutating the digestion site of dipeptidyl peptidase in GLP-1 from alanine to glycine, ligating with human albumin gene, and expressing in Pichia expression system to obtain four fusion proteins, which comprise recombinant GLP-1, human albumin and flexible linkers. The fusion proteins have such advantage as retained GLP-1 activity, long half-life period, good hypoglycemic effect, and no hypoglycemia phenomenon.

The invention discloses a low-temperature degrading method of halogenous plastic. According to the low-temperature degrading method, PVC (polyvinyl chloride) is adopted as a raw material, aluminite powder is adopted as a catalyst, aluminum trichloride and iodine are adopted as inducing agents, in an activation solvent system, the raw material, the catalyst and the inducing agents are enabled to react so as to generate the aluminum trichloride and a halogen-free carbon-containing compound at a temperature of 43-100 DEG C. The low-temperature degrading method of the halogenous plastic has the advantages that the problems that most of halogenous plastic require to be degraded under a high-temperature pyrolyzing condition at the temperature of 800 DEG C, nitrogen protection is required and produced hydrochloric acid is highly corrosive are solved, energy consumption can be reduced, equipment can be protected from being corroded, the aluminite powder can be converted into anhydrous aluminumtrichloride after reaction is cover, the problem of difficulty in halogenous plastic degradation is solved, and higher economic value is obtained through plastic degradation.

The invention relates to the field of construction additives, and in particular relates to a conditioning agent for residue soil. The conditioning agent for the residue soil comprises the following raw materials: a solid material and a liquid material; and the solid material comprises the following components, in percentages by weight, 1.4%-18.0% of polyacrylamide, 5.7%-72.1% of nonionic celluloseether, 9%-21.4% of an inorganic base, and 0.9%-71.4% of a sulfonic acid surfactant. The conditioning agent provided by the invention solves the problems of fluidity, soil discharge and degradation bymicroorganisms in soil of a sandy gravel stratum containing relatively large particles.