50results about How to "Solve degradation" patented technology

The invention discloses a recombinant human III-type collagen and a pichia pastoris recombinant expression system thereof. According to the invention, a Gly-X-Y structural peptide containing valine, phenylalanine, leucine, methionine and alanine in a part of peptide fragments of human III-type collagen is replaced with a Gly-X-Y structural peptide which is high in use frequency and does not contain the amino acid, and a gene sequence for encoding the peptide fragments is obtained according to the peptide fragments after the replacement. And then gene expression vectors for tandem expression ofpeptide fragments with different repeat numbers are constructed and transformed into pichia pastoris host bacteria, and a strain for high expression of the recombinant human III-type collagen is obtained through screening. Through artificial design optimization of a protein structure, the problem that natural collagen is degraded in fermentation is effectively solved, and an expression product has high stability and good biocompatibility and can be used for large-scale production.

The invention relates to a clopidogrel bisulfate tablet. A preparation method of the clopidogrel bisulfate tablet comprises the following steps of mixing clopidogrel bisulfate, lactose and hydroxy propyl cellulose according to a weight ratio of 1: (0.02 to 1): (1 to 3), preparing the mixture into pellets, dressing the pellets, and pressing the dressed pellets and an appropriate amount of magnesium stearate into tablets. Compared with the prior art, the preparation method adopts a pellet dressing and tabletting technology, improves product stability and curative effects, and prevents the sticking problem produced in drug tabletting.

This invention relates to gene cloning and gene expression, more specially, a method for expressing recombinant hypoglycemic peptide with long-lasting effects. The method comprises: mutating the digestion site of dipeptidyl peptidase in GLP-1 from alanine to glycine, ligating with human albumin gene, and expressing in Pichia expression system to obtain four fusion proteins, which comprise recombinant GLP-1, human albumin and flexible linkers. The fusion proteins have such advantage as retained GLP-1 activity, long half-life period, good hypoglycemic effect, and no hypoglycemia phenomenon.

The invention relates to a vertical type animal gastrointestinal bionic digester, which comprises a motor, a connecting shaft sleeve, a heightening transmission rod, a stirring rod and a simulated digestive tract, wherein the connecting shaft sleeve is sleeved on an output shaft of the motor and synchronously rotates along with the motor, and the top end of the heightening transmission rod is fixedly sleeved in a sliding chute below the connecting shaft sleeve by a screw rod, so that the heightening transmission rod can move up and down in the connecting shaft sleeve; a locking nut is arranged on the heightening transmission rod and is used for adjusting the locking degree of the heightening transmission rod and the stirring rod, and the simulated digestive tract is a double-layer hollow vertical glass tube; the stirring rod is arranged in an accommodating cavity of the simulated digestive tract, and the top end of the stirring rod penetrates through a sealing plug at the top of the simulated digestive tract and is connected with the bottom end of the heightening transmission rod. The vertical type animal gastrointestinal bionic digester provided by the invention has the advantages of simple structure, convenience in use, avoidance of leakage of a bionic digestion reaction liquid, high accuracy and good repeatability of digestion simulation, and small errors; the degradation difficulty of cellulose on a dialysis bag in the existing simulated digestive tract in a bionic digestion reaction is effectively solved.

The invention belongs to the field of biodegradable plastics, and discloses a method for preparing attapulgite-based biodegradable plastics from a natural attapulgite-loaded biopolymer material and a photocatalytic material. Natural attapulgite is subjected to functionalization treatment to serve as a carrier, a biological polymer material and a photocatalytic material are adsorbed through a physical/chemical method, modified attapulgite composite powder is prepared, the modified attapulgite composite powder and biodegradable plastic powder are blended to prepare a modified master batch, and the attapulgite-based biodegradable plastic which is low in cost, good in mechanical property and high in degradation rate is prepared through processing and forming. The attapulgite-based biodegradable plastic prepared by the method has the characteristics of good mechanical property, low cost, high biological decomposition rate and the like, can be applied to replace traditional non-degradable plastic, and provides a new way for solving the problem of white pollution.

The invention relates to the maritime aerial image defogging field and discloses an adaptive weight dark channel defogging algorithm for maritime aerial images of an unmanned aerial vehicle. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle fully considers the maritime aerial image defogging specificity, to be specific, the adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle does not only consider the effective defogging for a maritime specific object but also considers the fast, effective and correct defogging for a sea surface region in an image. The adaptive weight dark channel defogging algorithm combines foggy image quality degradation features with specific features of the sea area in the maritime aerial image, abandons a traditional defogging method, and fully considers the specificity of the sea area in the maritime foggy image. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle mainly includes that acquiring maritime aerial images of the unmanned aerial vehicle, performing dark channel defogging algorithm, performing adaptive weight algorithm, and defogging the maritime aerial images of the unmanned aerial vehicle. The adaptive weight dark channel defogging algorithm for the maritime aerial images of the unmanned aerial vehicle realizes the fast defogging for the maritime aerial images of the unmanned aerial vehicle and has broad prospect in the maritime or island aerial monitoring aspect.

The invention provides a VOCs waste gas treatment method combining absorption and adsorption. The method includes the following steps: absorbing VOCs exhaust gas in a first absorption tower under normal temperature and normal pressure, extracting non-standard gas from the top of a tower, introducing the non-standard gas into a rotating wheel for concentration or other adsorption systems, discharging purified gas reaching the standard directly, discharging concentrated gas with VOCs into a second absorption tower after residual VOCs in the non-standard gas is concentrated by absorption-adsorption, discharging the gas absorbed and purified in the second absorption tower after the gas reaches the standard, combining a first absorbent pregnant solution of the first absorption tower and a second absorbent pregnant solution of the second absorption tower, exchanging heat between the combination and the regenerated lean liquid extracted from a desorbing tower, then introducing the combinationinto the desorbing tower after overheating for steam stripping and regeneration in the tower. The treatment method is suitable for exhaust gas in low, medium and high concentration, and has remarkable effects on treatment of the exhaust gas with VOCs concentration below 5000 mg/m3.

The invention discloses a method for extracting total RNA (Ribonucleic Acid) of grapholita molesta adult antennae. The method comprises the following steps of: freezing grapholita molesta adults for coma under a low temperature condition, shearing a plurality of antennae by using small medical scissors and forceps, immediately soaking the antennae in a 1.5ml centrifugal tube in liquid nitrogen, taking the centrifugal tube out of liquid ammonia, immediately adding a small amount of RNA extraction liquid, fully homogenizing the extraction liquid by using a homogenizer, adding enough total RNA extraction liquid, blowing for a few times by use of a pipettor, transferring to the other new centrifugal tube, performing chloroform extraction according to a total RNA extraction method, and performing isopropanol and ethanol precipitation and separation, thus obtaining high-purity total RNA. The method is simple and convenient, and can be used for solving the problem about degradation of the RNA in the extraction process, so that the high-quality total RNA is obtained.

The invention discloses a decomposing method of viscous oil effluent chemical oxygen demand, which is characterized by the following: fetching water from anaerobic pond; adding H2O2 solution and FeSO4 solution sequently; blending to react with effluent; making effluent not generate toxic material; dissolving water in the oil acid, alcohol and water; depositing after 2h; forming transparent non-odor oil chemical oxygen demand water to satisfy draining standard.

The invention discloses a recombinant III-type humanized collagen, a nucleic acid, a carrier and an implant, and relates to the technical field of biological genes. Sequence peptide fragments 549-560aa, 597-613aa, 881-902aa and 648-662aa which are obtained by screening a natural human III type collagen alpha1 chain for a long time and have good stability are selected, the four peptide fragments are spliced in sequence to form monomers, and then tandem expression is performed on the monomers. Through fermentation and purification, the obtained recombinant III-type humanized collagen is high in stability, the problem of degradation of the recombinant III-type humanized collagen in the clinical use process is effectively solved, and the recombinant III-type humanized collagen can be prepared into a liquid preparation for preservation and use.

The invention discloses a method used for processing methyl orange dye waste water with bean product waste water and waste residue. According to the method, methyl orange dye waste water is treated with waste water and waste residue generated in bean product processing process, so that methyl orange dye waste water degradation is realized, and a part of toxic harmful substances generated by secondary oxidation decomposition of bean product waste water is reduced. Soybean contains a large amount of unsaturated fatty acids including oleic acid, linoleic acid, and linolenic acid, in processing process, oxidation reaction of those unsaturated fatty acids under lipoxygenase enzyme catalytic oxidation and thermopolymerization conditions is easily caused, free radicals and hydroperoxides with high reaction capacity, and secondary oxidation products such as aldehyde and ketone generated through decomposition of the hydroperoxides are generated, and enter into bean product waste water and wasteresidue, oxidation degradation of methyl orange solution is realized, decolouring effect is achieved, and the application prospect in the field of waste water processing in environment protection ispromising.

The invention discloses a low-temperature degrading method of halogenous plastic. According to the low-temperature degrading method, PVC (polyvinyl chloride) is adopted as a raw material, aluminite powder is adopted as a catalyst, aluminum trichloride and iodine are adopted as inducing agents, in an activation solvent system, the raw material, the catalyst and the inducing agents are enabled to react so as to generate the aluminum trichloride and a halogen-free carbon-containing compound at a temperature of 43-100 DEG C. The low-temperature degrading method of the halogenous plastic has the advantages that the problems that most of halogenous plastic require to be degraded under a high-temperature pyrolyzing condition at the temperature of 800 DEG C, nitrogen protection is required and produced hydrochloric acid is highly corrosive are solved, energy consumption can be reduced, equipment can be protected from being corroded, the aluminite powder can be converted into anhydrous aluminumtrichloride after reaction is cover, the problem of difficulty in halogenous plastic degradation is solved, and higher economic value is obtained through plastic degradation.