13 results about "Nonspecific adsorption" patented technology

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

A particle includes a copolymer including a repeating unit A having, in a side chain, a sulfide group or a secondary amine, and three or more hydroxy groups, and a repeating unit B having, in a side chain, at least one reactive functional group for binding a ligand, in which nonspecific adsorption is suppressed.

Provided are a test reagent that is reduced in nonspecific adsorption and is capable of detecting a low-concentration target substance, and a detection method including using the test reagent. The test reagent is a test reagent for in vitro diagnosis including: a thiourea compound; and resin particles, wherein the resin particles each have a sulfur element.

The invention discloses an anti-pollution and sterilization integrated non-co-reactant electrochemical luminescence (ECL) sensor for detecting staphylococcus aureus in an aquatic product as well as a preparation method and an application method of the anti-pollution and sterilization integrated non-co-reactant ECL sensor, and the anti-pollution and sterilization integrated non-co-reactant ECL sensor is suitable for ultra-sensitive detection of the staphylococcus aureus. The integrated hydrogel illuminant is characterized in that chitosan and citral are subjected to a Schiff base reaction to form a three-dimensional network in a cross-linking manner, and tetrakis (4-hydroxyphenyl) porphyrin (THPP) and luminol are introduced into the three-dimensional network. According to the present invention, THPP is adopted as a high efficiency oxygen evolution reaction (OER) electrocatalyst to generate reactive oxygen species (ROS) to replace the traditional hydrogen peroxide so as to be adopted as a co-reactant, such that the ECL signal of luminol is significantly enhanced; the hydrogel has excellent hydrophilicity and antibacterial activity, can significantly reduce non-specific adsorption, realizes anti-pollution detection, and realizes sterilization treatment after detection. By combining a DNA Walker signal amplification strategy, the sensor has a linear response to S. aureus within the range of 101-108 CFU / mL, the detection limit is as low as 0.39 CFU / mL, and the recovery rate of 97.3%-111.85% is obtained in a marine product actual sample. The device has the advantages of being simple in structure, sensitive in detection, high in anti-interference capacity, capable of achieving on-site rapid detection and the like, and is suitable for the fields of food safety, environment monitoring and clinical pathogenic bacterium detection.

The invention discloses an HIV (Human Immunodeficiency Virus) detection reagent and a kit. The HIV detection reagent comprises a reagent buffer solution and a treatment solution, the reagent buffer solution comprises a buffer agent, inert protein, a surfactant and a preservative; the treatment fluid includes a protein denaturant. According to the detection reagent buffer solution, the problem of weak-binding non-specific adsorption can be remarkably solved on the premise that normal performance of a reagent is not affected, particularly, compared with an HIV detection kit in the prior art, the false positive problem of the HIV detection kit prepared from the detection reagent buffer solution is remarkably improved, and the specificity and detection accuracy of a product are effectively improved.

The present application relates to the field of phosphorylated tau protein detection, and particularly relates to a method for improving the sensitivity of phosphorylated tau protein detection and a kit thereof.The present application provides a method for improving the sensitivity of phosphorylated tau protein detection, comprising: S101 obtaining a blood sample; S102 adding a detection reagent to the blood sample; S103 detecting the concentration of phosphorylated tau protein in the blood sample using a chemiluminescence method; wherein the detection reagent comprises an alkaline phosphatase polymer-antibody complex.The alkaline phosphatase polymer-antibody complex prepared by the present application has high yield, good stability, high coupling efficiency, significantly improved sensitivity, low background, good precision, strong anti-interference ability, smaller batch difference and lower non-specific adsorption, and exhibits significant advantages in detecting phosphorylated tau protein in blood samples.

A particle includes a copolymer including a repeating unit A having, in a side chain, a sulfide group or a secondary amine, and three or more hydroxy groups, and a repeating unit B having, in a side chain, at least one reactive functional group for binding a ligand, in which nonspecific adsorption is suppressed.

The invention discloses tumor marker magnetic microsphere detection equipment supporting a medical health big data platform, and relates to the technical field of medical health. The tumor marker magnetic microsphere detection equipment is prepared by the following steps: constructing a multi-channel micro-fluidic chip and preprocessing magnetic microspheres; sample loading and hydrodynamic focusing are carried out; local microenvironment impedance is monitored in real time; fluorescence imaging and original signal acquisition; performing dynamic correction based on track-impedance coupling; calculating a self-adaptive nonlinear correction signal; performing big data platform aggregation and group offset compensation; outputting a result and generating a clinical report; preparing for self-cleaning and recycling of the chip; by integrating high-frequency impedance sensing, in-situ and real-time physical measurement of non-specific adsorption on the surface of each magnetic microsphere is realized, and interference is quantified into a monitoring factor; the signal correction process can be dynamically adjusted according to the directly and synchronously acquired interference degree information, and the limitation that the instantaneous state change of individual microspheres cannot be responded due to post-processing or averaging processing is solved.

This invention discloses a two-dimensional liquid chromatography (HPLC) method for determining lactoferrin in infant formula. Based on online purification HPLC technology, the first dimension uses a heparin affinity column, and the second dimension uses a reversed-phase C4 column. This method achieves accurate qualitative and quantitative analysis of lactoferrin in infant formula. By adding Tween-20 and sodium chloride to the extract, non-specific adsorption and competitive binding in complex matrices are effectively inhibited, significantly improving the recovery rate of lactoferrin (93.5%–97.3%). The method exhibits good linearity in the range of 5–200 μg / mL, with a detection limit of 2 mg / 100g, a quantitation limit of 5 mg / 100g, and an RSD of 1.2%–3.9%, providing accurate and reliable results. Optimized flow path and cleaning procedures enable online two-dimensional purification and analysis, allowing the expensive heparin affinity column to be reused and reducing detection costs.

This application relates to the field of in vitro bioassay technology, and discloses a method for preparing a colloidal gold assay reagent, its product, and its application. The method for preparing the colloidal gold assay reagent comprises five steps: colloidal gold preparation, antibody labeling, double blocking, centrifugal purification, and dilution and reconstitution. In particular, the double blocking process involves a first blocking agent selected from one or more of bovine serum albumin, ovalbumin, and casein; and a second blocking agent selected from one or more of lysine, glycine, alanine, serine, threonine, valine, and asparagine. This allows the prepared colloidal gold assay reagent to achieve high sensitivity and excellent C / T line color development when applied to FSH detection, reducing false positives caused by nonspecific adsorption and improving the accuracy of test results. Its limit of detection (LOD) is as low as 10 mIU / mL, solving the problems of low sensitivity and uneven C / T line color development in existing FSH assays.

The application discloses a kind of based on the rapid modification of multi-nucleic acid probe and multi-target detection device of electric field regulation and control.The instrument main body in the device integrates automatic liquid system and miniature electrochemical workstation module, for realizing programmable reagent and sample delivery and multi-channel electrochemical measurement.Detachable sensing chip is bonded by microelectrode array chip and microfluidic chip, and is connected by PCB board, to realize plug and play.The application uses electric field "positive suction-negative discharge" strategy, applies optimal positive potential to target electrode to efficiently attract probe, while applying optimal negative potential to non-target electrode to inhibit non-specific adsorption, so as to realize spatial selective rapid fixation and density accurate regulation of multi-nucleic acid probe in very short time.The device can simultaneously quantitatively detect multiple body fluid disease markers in multiple urine samples in a single operation, providing an efficient, accurate and portable multi-marker comprehensive analysis solution for early screening and instant diagnosis of tumors.

The invention belongs to the technical field of enzyme-linked immunosorbent assay, and relates to a kit for animal epidemic disease detection. The invention provides a kit for animal epidemic disease detection, the kit is an enzyme linked immunosorbent assay kit, the enzyme linked immunosorbent assay kit comprises a diluent for sample dilution, and the diluent comprises 5-10 g / L of bovine serum albumin and 10-30 g / L of a bovine serum albumin-polymer coupling body. According to the invention, the technical problem of high background signal or false positive caused by adsorption of non-specific IgG to a solid-phase carrier when an enzyme-linked immunosorbent assay kit is used for enzyme-linked immunosorbent assay (ELISA) detection is solved.

The present application relates to electrode material for measuring blood potassium content, preparation method, working electrode, electrode device and blood potassium content measuring system. Its advantages are that through the chemical performance improvement of the new material of the bottom electrode interface, the selectivity, sensitivity and detection upper limit of the electrochemical sensor (electrode device) in the complex blood environment are significantly improved, so as to meet the accurate monitoring demand of the clinical household blood potassium range; the high molecular weight polymer forms a more dense and stable electrode film, which realizes unprecedented close and firm attachment with the electrode base, fundamentally avoids the swelling or peeling of the electrode film in the detection process, and ensures the long-term stability of the signal; the dense and regular chemical structure interface layer greatly improves the specific selection ability of the electrochemical sensor (electrode device) to potassium ions, and effectively shields the non-specific adsorption interference of common coexisting ions and biological macromolecules such as proteins and lipids in the blood sample.