59 results about "Nonspecific adsorption" patented technology

A coated container having reduced nonspecific adsorption of nonspecific targets can have an interior volume surrounded by one or more walls having one or more interior surfaces, and a coating comprising a spray-coated hydrogel layer surface attached to the one or more interior surfaces, the spray-coated hydrogel having surface roughness with surface features with an average features height of less than 20 nm.

The present invention provides a protein detection method and detection preparation, which realizes efficient enrichment of target protein through mesoporous silica-graphene oxide composite nanoporous material, completes specific immune capture in combination with magnetic nanoparticles modified with targeted antibodies, and realizes single molecule separation using a microfluidic chip of a three-dimensional hydrogel antibody array. Signal amplification adopts enzyme-labeled secondary antibody-gold nanorod conjugate, enhances the fluorescence signal through the plasma resonance effect, and corrects the background noise in combination with a convolutional neural network algorithm, which significantly improves the detection reliability; experiments show that the detection limit of this method is as low as 0.12pg / mL, the linear range covers 0.5-100,000pg / mL, the single cell detection error is ±7.8%, the batch repeatability (CV=4.2%), the nonspecific adsorption rate is only 1.1%, and the detection time is shortened to 1.2 hours. The detection method of the present invention is suitable for ultra-sensitive detection of tumor markers, especially in the in situ analysis of CTCs with clinical application potential.

The invention relates to the technical field of temperature-sensitive gel, in particular to a dental plaque temperature-sensitive gel color developing agent. The temperature-sensitive gel color developing agent for dental plaque comprises the following components in percentage by mass volume: 0.4-5% of food coloring; 0.5%-2% of a charge protective agent; 0.5%-3% of an adhesive material; 10%-55% of a temperature-sensitive material; 0.05%-0.3% of a bacteriostatic agent and a proper amount of water. The color developing agent is safe and non-toxic, is a flowing liquid at room temperature, is converted into a gel solid from a liquid state when the color developing agent is coated on the tooth surface to reach a phase transition temperature, can effectively reduce the problem of non-specific adsorption compared with a liquid color developing agent, and is easy to remove after being used.

The present invention discloses a method for preparing water-soluble quantum dots. The preparation method uses a surfactant solution to coat oil-soluble quantum dots, retaining the fluorescence of the oil-soluble quantum dots, and uses water-soluble polymer ligands (polyvinyl alcohol and polyvinyl pyrrolidone) to complete functional modification, thereby solving the problems of easy quenching of quantum dot fluorescence, inability of nanoparticles to stably agglomerate, and prone to nonspecific adsorption, resulting in low detection sensitivity. When the water-soluble quantum dots prepared by the preparation method of the present invention are used for biomarker detection, they can be directly dissolved in water, and the fluorescent group is stable, can stably agglomerate and specifically adsorb biological targets; therefore, the immunochromatographic test strips prepared using the water-soluble quantum dots can simply and stably adsorb drug antibodies, thereby improving detection sensitivity, with the detection sensitivity for morphine reaching 0.1 ng / mL.

The invention relates to a preparation method of a silica ball surface coated polymer and bonded functional stationary phase chromatography filler. The preparation method comprises the following steps: S1, silica gel etherification; s2, coating a polymer on the surface of the vinylated silica gel; and S3, bonding octadecyl or aminopropyl, and performing functional modification on the silicon-based material of which the surface is coated with the polymer to obtain the required silica gel matrix chromatographic filler. According to the preparation method of the silica ball surface coated polymer and bonded functional stationary phase chromatographic filler, the pH range used by silica gel matrix chromatographic filler is expanded, and meanwhile, the polymer on the surface effectively covers silicon hydroxyl on the silica gel surface, so that non-specific adsorption of strong-polarity and alkaline substances is avoided, and the service life of the silica gel matrix chromatographic filler is prolonged. And the separation efficiency of the filler can also be obviously improved.

A coated metallic base comprising a metallic base being a metallic nanoparticle and / or a metallic film, a polymer layer covering a surface of the metallic base, and a first hydrophilic group that inhibits nonspecific adsorption to the surface of the metallic base, the first hydrophilic group being bonded to the surface of the metallic base, and the polymer layer containing a binding site with a sulfur atom interposed therein between the polymer layer and the surface of the metallic base.

The present invention discloses a lactic acid detection kit and a preparation method thereof, which belongs to the technical field of detection reagents. The kit comprises 3 mL of R1 reagent, 1 mL of R2 reagent and 1 mL of standard product. The R1 reagent contains bovine serum albumin, nanosilver-trehalose complex and PEG4000. The bovine serum albumin can prevent enzyme decomposition and nonspecific adsorption, chelate a small amount of dissociated Ag, and + , preventing competition with H2O2 for the binding sites of horseradish peroxidase and ensuring the stable generation of quinoneimine; PEG4000 plays a dispersing role, assisting the nanosilver-trehalose complex to form a protective film on the surface of lactate oxidase and horseradish peroxidase, protecting the protein structure of the two enzymes, and can capture electrons from the surrounding environment, increasing the electron transfer efficiency of the active centers of the enzymes during the catalytic process of the two enzymes, thereby further increasing the activity of the two enzymes and improving the sensitivity and accuracy of the kit.

The invention discloses a preparation method of a material for enriching hydrophilic biological substances, which is characterized in that peptide fragments are enriched through specific binding of chemically modified magnetic nanoparticles and glycosylated peptide fragments, so that the problems of high cost, complicated operation and limited identification flux are solved; and an efficient, stable and easy-to-operate technical process is provided for research on glycoproteomics. The surface of the magnetic bead is a combination of a mesoporous structure and a quaternary ammonium salt group, so that the selectivity on a glycosylated peptide fragment is remarkably improved, and non-specific adsorption is reduced. The number of N-glycopeptides can be averagely identified to 2733, and the number of O-glycopeptides can be averagely identified to 1777. The enrichment process is simplified by introducing the magnetic beads, and subsequent automatic experiments and large-scale application are facilitated. And the cost is reduced: the preparation and application cost of the material is reduced by simplifying the synthesis method and improving the stability of the material.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

A particle includes a copolymer including a repeating unit A having, in a side chain, a sulfide group or a secondary amine, and three or more hydroxy groups, and a repeating unit B having, in a side chain, at least one reactive functional group for binding a ligand, in which nonspecific adsorption is suppressed.

The invention provides a preparation method and application of a non-specific protein adsorption resistant coating based on a normal-pressure low-temperature plasma enhanced chemical vapor deposition technology. According to the preparation method, the stable protein nonspecific adsorption resisting ultrathin coating can be rapidly modified on the surface of the substrate in a normal-pressure environment, and a covalent bond is formed with the surface of the substrate. The preparation method of the non-specific protein adsorption resistant coating provided by the invention is simple to operate and wide in applicable material range, the biocompatibility and blood compatibility of the surface of a medical instrument can be improved, and the problems of coagulation and thrombus caused by non-specific protein adsorption, biological membrane infection caused by bacteria attachment growth and the like are reduced; and the stable covalent bond combination and the ultrathin coating reduce the risk caused by coating falling in the use process.

The invention discloses a detection method and a detection kit for a milligram-scale drug-resistant antibody, and belongs to the field of biological drug immunogenicity analysis and detection.According to the detection method and the detection kit, a milligram-scale ADAs drug-resistant sample is detected only by acidizing the sample once and using a high-concentration bridging reagent and low-nonspecific adsorption nano magnetic particles, and high-concentration drugs in the sample do not need to be separated and removed; therefore, the detection of the milligram-scale drug-resistant antibody can be realized. The method can overcome the defect that the milligram-grade drug-resistant sample can be detected and analyzed only by separating and removing drugs and acidizing twice in the prior art, and greatly improves the detection efficiency of the drug-resistant drug-resistant antibody.

The present invention belongs to the field of electrochemical detection technology and relates to a new anti-fouling material OPA-CsPbBr3, its preparation method, and sensing application. The present invention forms the anti-fouling material OPA-CsPbBr3 by doping OPA polymer into CsPbBr3. On the one hand, due to the good hydrophilicity and near-neutrality of OPA, it exhibits excellent anti-fouling performance; on the other hand, it can further improve the water stability of CsPbBr3, so that it exhibits excellent glucose oxidase-like activity. The present invention further provides an anti-fouling electrochemical biosensor using OPA-CsPbBr3 as an anti-fouling material and peptide nucleic acids (PNAs) as sensing probes. The sensor can effectively resist the nonspecific adsorption of biological molecules such as proteins in complex biological samples (such as blood and serum). In addition, the integrated nanozyme cascade catalysis and CRISPR / Cas13a system further enhance the detection sensitivity of the anti-fouling electrochemical biosensor, enabling sensitive detection of miRNA-let-7a in complex biological samples.

Provided are a test reagent that is reduced in nonspecific adsorption and is capable of detecting a low-concentration target substance, and a detection method including using the test reagent. The test reagent is a test reagent for in vitro diagnosis including: a thiourea compound; and resin particles, wherein the resin particles each have a sulfur element.

The present invention discloses a plasmon-coupled dynamic, non-copyable anti-counterfeiting label. The preparation method comprises the following steps: first, providing a photoresist layer on a silicon wafer substrate and patterning the wafer using photolithography; second, sequentially applying an aluminum film and a silver film by magnetron sputtering or thermal evaporation; third, dropwise adding a silver nanoparticle solution onto the silver film surface; the silver nanoparticles assemble onto the silver film in a random distribution through nonspecific adsorption and exhibit optical behavior response under detection light of a preset wavelength; and fourth, removing the photoresist layer on the silicon wafer substrate using an organic solvent, thereby producing a plasmon-coupled dynamic, non-copyable anti-counterfeiting label. The anti-counterfeiting label preparation method disclosed herein is simple, requires minimal equipment, and has a high detection speed, offering excellent development prospects in anti-counterfeiting.

The present invention provides a method for screening anti-nonspecific adsorption reagents for FET biosensors. By combining reagents with different terminal functional groups, charges and hydrophilicity, and through the synergistic effects of hydrogen bonds, steric effects and electrostatic repulsion, multi-attribute and multi-ratio anti-nonspecific adsorption reagents for target detection systems are screened. The process is controllable and highly targeted, and anti-nonspecific adsorption reagents with excellent effects are screened more accurately for target detection systems, providing guarantees for subsequent detection. The anti-nonspecific adsorption reagents screened by the method of the present invention can improve the hydrophilicity of the interface, produce steric effects, and enhance electrostatic repulsion through the interaction between the different terminal functional groups of the thiol compound reagents, thereby reducing the adsorption of non-targets in the target detection system and showing strong anti-fouling performance. The modified FET biosensor has a wider linear detection range and an extremely low detection limit, strong anti-interference ability, high sensitivity, and fast response time.

The invention discloses an anti-pollution and sterilization integrated non-co-reactant electrochemical luminescence (ECL) sensor for detecting staphylococcus aureus in an aquatic product as well as a preparation method and an application method of the anti-pollution and sterilization integrated non-co-reactant ECL sensor, and the anti-pollution and sterilization integrated non-co-reactant ECL sensor is suitable for ultra-sensitive detection of the staphylococcus aureus. The integrated hydrogel illuminant is characterized in that chitosan and citral are subjected to a Schiff base reaction to form a three-dimensional network in a cross-linking manner, and tetrakis (4-hydroxyphenyl) porphyrin (THPP) and luminol are introduced into the three-dimensional network. According to the present invention, THPP is adopted as a high efficiency oxygen evolution reaction (OER) electrocatalyst to generate reactive oxygen species (ROS) to replace the traditional hydrogen peroxide so as to be adopted as a co-reactant, such that the ECL signal of luminol is significantly enhanced; the hydrogel has excellent hydrophilicity and antibacterial activity, can significantly reduce non-specific adsorption, realizes anti-pollution detection, and realizes sterilization treatment after detection. By combining a DNA Walker signal amplification strategy, the sensor has a linear response to S. aureus within the range of 101-108 CFU / mL, the detection limit is as low as 0.39 CFU / mL, and the recovery rate of 97.3%-111.85% is obtained in a marine product actual sample. The device has the advantages of being simple in structure, sensitive in detection, high in anti-interference capacity, capable of achieving on-site rapid detection and the like, and is suitable for the fields of food safety, environment monitoring and clinical pathogenic bacterium detection.

The invention discloses an HIV (Human Immunodeficiency Virus) detection reagent and a kit. The HIV detection reagent comprises a reagent buffer solution and a treatment solution, the reagent buffer solution comprises a buffer agent, inert protein, a surfactant and a preservative; the treatment fluid includes a protein denaturant. According to the detection reagent buffer solution, the problem of weak-binding non-specific adsorption can be remarkably solved on the premise that normal performance of a reagent is not affected, particularly, compared with an HIV detection kit in the prior art, the false positive problem of the HIV detection kit prepared from the detection reagent buffer solution is remarkably improved, and the specificity and detection accuracy of a product are effectively improved.

The present application relates to the field of phosphorylated tau protein detection, and particularly relates to a method for improving the sensitivity of phosphorylated tau protein detection and a kit thereof.The present application provides a method for improving the sensitivity of phosphorylated tau protein detection, comprising: S101 obtaining a blood sample; S102 adding a detection reagent to the blood sample; S103 detecting the concentration of phosphorylated tau protein in the blood sample using a chemiluminescence method; wherein the detection reagent comprises an alkaline phosphatase polymer-antibody complex.The alkaline phosphatase polymer-antibody complex prepared by the present application has high yield, good stability, high coupling efficiency, significantly improved sensitivity, low background, good precision, strong anti-interference ability, smaller batch difference and lower non-specific adsorption, and exhibits significant advantages in detecting phosphorylated tau protein in blood samples.

An object of the present invention is to provide a kit, a method, and a reagent which prevent the problem of false positive due to nonspecific adsorption, suppress the increase in noise to be generated, and are capable of achieving high-precision measurement of progesterone in a wide concentration range from a low concentration to a high concentration, in the measurement of progesterone in a biological sample. The present invention provides a progesterone measuring kit including a first particle having a label and modified with a first binding substance, a second particle having no label and modified with a second binding substance, a flow channel, and a substrate, in which the first particle contains a compound represented by Formula (1) and a particle, and an average particle diameter of the first particles is 50 to 250 nm, an average particle diameter of the second particles is 70 to 500 nm, and the average particle diameter of the second particles is larger than the average particle diameter of the first particles.Each symbol in the formula has the meaning described in the present specification.

The invention discloses a digital method for evaluating non-specific adsorption of a bioactive substance diluent, belongs to the technical field of immunodetection, and relates to a digital method for evaluating non-specific adsorption of a bioactive substance diluent by establishing a digital fluorescence immunoadsorption analysis system. The influence of a certain step or a certain material on non-specific adsorption in the experiment process is evaluated by replacing a bioactive substance sample diluent, so that the non-specific adsorption in the experiment process is evaluated, the experiment method is perfected, and the detection accuracy is improved.

The invention relates to the field of nano biomedical photosensitive diagnosis and treatment, in particular to a cyanine near-infrared contrast agent as well as a preparation method and application thereof. The cyanine near-infrared contrast agent provided by the invention has an amphiphilic molecular structure, so that the stability of fluorescence can be ensured, and non-specific adsorption is reduced through a hydrophilic chain. Meanwhile, hyaluronic acid is specifically combined with a CD44 receptor on the surface of a tumor cell, so that a contrast agent can be guided to preferentially gather in a tumor tissue, the targeting property to the tumor tissue is improved, and the toxicity caused by dissociation of near-infrared cyanine dyes is reduced. In addition, interference of background fluorescence is reduced due to high tumor targeting, and the signal-to-noise ratio and the imaging effect of NIR-II are improved. Besides, hyaluronic acid has good water solubility, and after water-soluble modification is carried out on the cyanine molecules, the cyanine molecules have good biocompatibility, immune system recognition is reduced, and attack by an antibody or complement system is avoided.

The invention belongs to the technical field of biological medicine micro-nano materials, and particularly relates to a magnetic microsphere with protein nonspecific adsorption resistance for immunodiagnosis as well as a preparation method and application of the magnetic microsphere. The preparation method comprises the following steps: S01, carrying out free radical copolymerization reaction on magnetic microspheres and a cross-linked copolymer material under the action of an initiator to introduce carboxyl to obtain first magnetic microspheres; s02, carrying out covalent coupling on the first magnetic microspheres, micromolecules or polymers with polyamino functional groups and micromolecules or polymers with polycarboxyl functional groups to obtain second magnetic microspheres; S03, adding zwitterions into the reaction system, and obtaining third magnetic microspheres on the basis of the second magnetic microspheres; and S04, adding a functional monomer with a carboxyl group or an epoxy group into the reaction system, and obtaining a final compound microsphere on the basis of the third magnetic microsphere. The composite magnetic microspheres provided by the invention can be used for immunodetection, and have higher signals and lower background.

The invention discloses a biosensor measuring method based on a two-dimensional miniature field effect transistor electrode. The method comprises the following steps: firstly, cleaning the sensor with acetone / isopropanol / deionized water, and annealing in argon environments of 150 DEG C and 180 DEG C to reduce contact resistance; then, through oxygen plasma surface activation, APTES amination and glutaraldehyde crosslinking, the antibody is fixed on the surface of the aluminum oxide protection layer; bSA sealing is adopted to reduce non-specific adsorption; and finally measuring electric signal response by using a source meter under the condition of control voltage. The method is standard in process and clear in parameter, and the detection sensitivity, specificity and repeatability are remarkably improved.

To provide a particle for a latex agglutination method that has a reactive functional group for chemically coupling a ligand and has small nonspecific adsorption, and that can be used for inspection particles excellent in dispersion stability when stored standing, that is, excellent in standing storage stability.SOLUTION: A particle has a polymer including a structural unit A represented by a formula (1) and a structural unit B represented by a formula (2) (for the formula (1) and the formula (2), refer to the specifications). The specific gravity of the particle is within a range of 1.00 or more and 1.10 or less. The composition ratio of a C element to an O element quantified by XPS measurement is within a range of 2.1 or more and 3.3 or less.SELECTED DRAWING: Figure 1

A particle for latex agglutination method that can be used as a test particle, having a reactive functional group for chemically bonding to a ligand, with a small nonspecific adsorption, excellent in dispersion stability after being stored standing still, i.e., excellent in static storage stability is provided. The test particle has a ligand, and a particle chemically bonded to the ligand, wherein the particle has a polymer including a structural unit A represented by formula (1) and a structural unit B represented by formula (2) (refer to specification) and has a specific gravity in the range of 1.00 or more and 1.10 or less, and has a composition ratio of element C to element O quantified by XPS measurement in the range of 2.1 or more and 3.3 or less.

The invention relates to the technical field of biological detection, in particular to a detection reagent for biological analysis, a kit and application, the detection reagent comprises a regulating medium, and the regulating medium comprises a buffer system. Through innovative component combination and formula optimization, non-specific adsorption is reduced, the signal-to-noise ratio is increased, the detection sensitivity is improved, particularly, a specific amino acid buffer solution is introduced, the specific amino acid buffer solution can interact with a hydrophobic region on the surface of protein, non-specific adsorption components are reduced, and the biological analysis and detection performance can be remarkably improved.

The invention provides adsorption resin for treating lupus erythematosus and a preparation method of the adsorption resin, and relates to the technical field of preparation of medical drugs, agarose has a rich three-dimensional pore structure (the pore diameter gradient is 50-150 nm) and a high specific surface area (110 m / g), provides sufficient antibody diffusion channels and ligand binding sites, realizes advantage complementation of the two materials, and has a good application prospect in treatment of lupus erythematosus. Meanwhile, porous microspheres with the aperture gradient distributed in the range of 30-200 nm are formed through five times of 100 MPa circulating high-pressure homogenization treatment, the dynamic adsorption capacity of the hybrid carrier is improved by 30%-50% compared with that of a single material carrier, non-specific adsorption of multiple proteins in plasma is inhibited through a zwitterionic polymer coating (such as sulfobetaine methacrylate), and the dynamic adsorption capacity of the hybrid carrier is improved by 30%-50%. The albumin loss rate is further reduced to 1.5% or below, the bacterial colonization risk in the perfusion process is reduced through the natural antibacterial activity of chitosan, ligands are fixed to the surface of an aminated carrier through covalent bonds by means of an EDC / NHS coupling system, one order of magnitude is reduced compared with the prior art, and the secondary immune reaction risk is effectively avoided.

A particle includes a copolymer including a repeating unit A having, in a side chain, a sulfide group or a secondary amine, and three or more hydroxy groups, and a repeating unit B having, in a side chain, at least one reactive functional group for binding a ligand, in which nonspecific adsorption is suppressed.

The invention belongs to the technical field of SPR (Surface Plasmon Resonance), and particularly relates to a surface plasmon element resonance chip for cell fixation and a preparation method thereof. The chip comprises a specific adsorption component and an anti-nonspecific adsorption component, and the specific adsorption component and the anti-nonspecific adsorption component are distributed on the surface of the chip in a layered manner and are matched with each other to realize fixation of cells on the surface of the chip and control of nonspecific adsorption. The preparation method comprises the following steps: firstly bonding long-chain alkane on the surface of the chip through Au-S to obtain the hydrophobic support layer, then grafting glucan or nanocellulose through reaction among various active groups to obtain the stable layer, and grafting adhesive protein and the like through reaction among the active groups to obtain the binding layer. Under the condition that non-specific adsorption on the surface of the chip is effectively controlled, the requirement of cell fixation is met; the surface of the chip has good cell compatibility and can provide conditions for adhesion, survival, proliferation and differentiation of cells; the fixation and survival rate of cells on the surface of the modified chip are obviously improved.