1279 results about "Bio sensor" patented technology

The invention discloses a nanopore sensor based on insect olfactory receptor protein as well as a construction method and application thereof, and belongs to the field of biosensors. According to the invention, the insect olfactory receptor protein is used as a sensitive element to be integrated into a nanopore measurement system, so that the nanopore sensor is obtained. By measuring a nanopore current signal, the prepared sensor is applied to detection of target odorant molecules. According to the method, the recognition capability of the insect olfactory receptor protein on odorant molecules and the single molecule level detection capability of the nanopores are utilized, expensive large experimental instruments and complex experimental operation steps are not needed, and sensitive, rapid and direct detection on odorant substances can be realized.

The invention relates to the technical field of treatment of chronic diseases of old people, and discloses a metabolic characteristic-based medicine dosage optimization method for chronic diseases of old people. The method comprises the following steps: acquiring drug metabolism parameters (including a plasma concentration peak value, a half-life period curve and the like) and organ function data (including a hepatocyte metabolism rate, a glomerular filtration rate and the like) of a plurality of monitoring nodes, arranging the drug metabolism parameters and the organ function data into a time sequence input vector, and extracting a dynamic feature vector by using a time convolution network and an adaptive filter network; performing feature crossing, pharmacokinetic constraint correction and feature enhancement processing, performing fusion to generate a joint feature vector, inputting the joint feature vector into a dose decision model to obtain an adjustment coefficient, and generating a drug dose interval with a safety threshold in combination with a historical drug use record. According to the method, multi-dimensional data integration and dynamic modeling are realized, the accuracy and safety of chronic disease medication of old people are improved, and the method is suitable for individualized treatment.

Antibodies and antibody fragments that bind to polyfluoroalkyl and perfluoroalkyl species (PFAS) are described for use in biosensors for detecting PFAS compounds in environmental samples. A detector comprising the biosensor and a kit for use with the detector are also described.

A biosensor cartridge can include a circuit board having a connection terminal configured to be electrically connected to an external diagnostic device, a biosensor chip configured to detect a target material from an analysis specimen, and transmit an electrical signal to the connection terminal of the circuit board based on the target material being present in the analysis specimen, the biosensor chip including a reactant configured to react specifically with the target material, and a housing enclosing the circuit board and the biosensor chip, and the connecting terminal being exposed outside of the housing. Also, the biosensor chip includes a plurality of channel areas separated from each other, and the reactant is attached to the plurality of channel areas.

The invention discloses a pheromone binding protein derived peptide capable of effectively monitoring sex pheromones of fall webworms and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, has no response to 17 plant volatile matters, and can detect sex pheromones released by as low as five live female fall webworms under laboratory conditions. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The invention relates to the technical field of health state monitoring, in particular to a real-time health state monitoring system and method based on a biosensor. According to the scheme, through multi-mode data fusion, physiological data such as postures, heart rates and blood oxygen collected by the biosensor are combined with postures and environment videos of image collection, the limitation of single data source monitoring is solved, and the situation that traditional falling early warning is misreported due to normal actions such as stooping is avoided; or altitude stress early warning only prompts oxygen deficit but cannot be associated with the falling risk. A pre-trained risk assessment model is combined to output grading risk levels, targeted early warning such as low-risk prompt deceleration and high-risk sound-light alarm is triggered, more accurate and timely health monitoring service is provided for the elderly in the plateau area, and the falling risk caused by interaction of the environment and the physiological state is effectively reduced.

The invention discloses a paper-based electrode based on liquid metal assisted laser-induced graphene and a preparation method thereof.The paper-based electrode comprises a substrate layer, a functional layer, a conductive layer and a hydrophobic layer which are sequentially arranged, the substrate layer is made of paper, the functional layer is formed by coating liquid metal nanoparticles, and the conductive layer is formed by coating liquid metal nanoparticles. The conductive layer is an electrode formed by a laser-induced graphene technology, and the hydrophobic layer is a fluid control area formed by wax packaging. According to the invention, paper is used as a substrate, liquid metal is combined with a laser-induced graphene technology, surface modification is carried out through eutectic gallium indium (EGaIn), polyimide (PI) and N-methyl pyrrolidone (NMP) composite slurry, and then a conductive electrode pattern is formed on the surface of the paper substrate by using a laser-induced graphene (LIG) technology. The method has the advantages of simple preparation, low cost, environmental friendliness and the like, and the prepared paper-based electrochemical biosensor has excellent conductivity, mechanical flexibility and electrochemical activity.

The invention discloses a staphylococcus aureus biosensor based on a nucleic acid aptamer and a preparation method of the staphylococcus aureus biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high-sensitivity detection of the staphylococcus aureus is achieved, the detection limit of the sensor within 5 min can achieve 5 cells / mL, the sensitivity is high, the specificity on the complex sample is more than 95.4%, and the sensor is suitable for the food safety rapid screening and the clinical diagnosis.

The present disclosure discloses a physiological disorder alert system (114) for alerting a subject. The system (112) includes a plurality of biosensor electrodes (102), and a controller (104). The plurality of biosensor electrodes (102) measure at least one physiological parameter of the subject (112). The controller (104) detects a plurality of anomalies present in the EEG signal based on comparing the EEG signal with an updated output from a predetermined longitudinal EEG using at least one of Artificial Intelligence model and determines a presence of a plurality of biomarkers associated with at least one anomaly in the EEG signal, using a transformer-based machine learning model. The controller predicts an occurrence of the physiological disorder, upon determining that the plurality of biomarkers associated with at least one anomaly is present in the EEG signal and generate the physiological disorder alert for a user equipment (UE) (116).

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention relates to the technical field of biosensors, and discloses a periodontal tooth health monitoring biosensor system. The system comprises a signal acquisition module, a feature extraction module, a core screening module and an anomaly judgment module. The signal acquisition module is used for acquiring multi-dimensional signals from a plurality of heterogeneous sensing terminals and integrating the multi-dimensional signals into a health monitoring signal database; a feature extraction module analyzes the database to obtain a health monitoring feature matrix, determines a correlation graph of each dimension and constructs a process correlation graph; a core screening module extracts health feature vectors, determines health index sensitive entropies, and screens out a health core feature sequence in combination with the process association map; and the abnormity judgment module carries out health abnormity judgment according to the sequence. According to the system, by integrating multi-dimensional signals, mining feature association and screening core features, precise monitoring and anomaly judgment of periodontal tooth health are achieved.

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

This invention is related to the field of biosensors. In particular, devices and methods are described which enable the quantification of biomolecules by virtue of particle speed driven by induced-charge electrophoresis. For example, a range of biomolecules can be simultaneously detected in a highly sensitive manner from a library (e.g., combination) of active particles having different shapes or fluorescent signatures. Such biomolecules are related to conditions including, but not limited to, cancer biomarkers, viral infection biomarkers, toxins, pesticides, and small molecule drugs.

The application discloses a malachite green label-free proportional biosensor based on multi-dimensional cutting aptamer, which comprises the following steps: (1) a performance-improved malachite green aptamer sequence; (2) a label-free proportional malachite green sensor sequence; and (3) malachite green detection. The biosensor is constructed by using various aptamer sequences, and the ideal sensing effect can be realized through the competition balance principle. The label-free proportional sensor can present a good linear relationship in the range of 5nM-4muM, and has a low detection limit of nanomolar level, which indicates that the sensor has good malachite green detection potential and can meet the application requirements.

The invention discloses a colorimetric aptamer sensor and application thereof, and belongs to the technical field of biological detection. The colorimetric aptamer sensor comprises Cas12a protein, HP, crRNA, dsDNA (double-stranded deoxyribonucleic acid) and a colorimetric probe. The colorimetric biosensor is applied to detection of the ochratoxin A, the content of the ochratoxin A is detected through the colorimetric biosensor, and detection and analysis of a target object can be achieved; cas12a aptamer inhibitors are developed as molecular switches for biosensors, which mediate target recognition through engineering modification of HP hairpin sequences. A new thought for improving the mycotoxin detection sensitivity is explored, and good detection potential is expected to be shown in the field of food safety.

A highly sealed invasive biosensing assembly and a biological information monitoring apparatus. The present invention relates to the technical field of biosensors. The highly sealed invasive biosensing assembly comprises a biosensor, and an electrode-protecting cover is snap-fitted to the lower part of the biosensor. A guide needle is snap-fitted into the biosensor, longitudinally penetrating the biosensor. The biosensor further comprises a sensing electrode and an electrode pressure plate. In a first state, the electrode pressure plate, the lower part of a needle guide seat, and the interior of the electrode-protecting cover form a first closed cavity. In a second state, the electrode-protecting cover is detached from the biosensor, so that at least part of the sensing electrode entering the first closed cavity and at least part of a needle body extend out of the first closed cavity. The invasive biosensing assembly and the biological information monitoring apparatus form a closed space inside the assembly and the apparatus by means of a snap structure and the positional relationship between components during assembly, thereby improving the hermeticity in the assembly and the apparatus, so that the assembly is in a sterile state before being implanted into a human body.

The invention relates to the field of biosensors, and discloses a cardiovascular data monitoring method for the cardiovascular medicine department. According to the method, electrocardio, respiration and blood oxygen signals are synchronously collected through a non-invasive terminal, sleep apnea events are analyzed and recognized in a combined mode, the electrocardio signals are deeply analyzed to detect cardiovascular abnormalities, and time sequence characterization of the function state of the autonomic nervous system is generated based on heart rate variability; and further constructing a time sequence causal reasoning model, quantifying the triggering effect of the respiratory event on the cardiovascular event, generating a comprehensive night cardiovascular risk layering index, and triggering graded early warning. According to the invention, non-sensitive, long-time-history and high-precision night cardiovascular risk dynamic assessment and causal mechanism analysis are realized.

The present invention relates to a system and method for generating lighting effects based on contextual data. The system includes one or more sources, including microphones, cameras, biometric sensors, environmental sensors, and digital feeds providing information including time, date, season, geolocation, weather, calendar events, or current events. A processor analyzes the contextual data to determine a contextual state associated with an ongoing event or user condition. The processor further identifies secondary trigger conditions through computer vision analysis of screen content, developer-scripted events, generative AI-derived cues, optical character recognition, gameplay events, or transformation of on-screen 2D or 3D visual regions. Based on the contextual or composite contextual state, the processor determines one or more lighting effects, which are then triggered on one or more output devices. This enables dynamic and contextually adaptive lighting responses to both real-world and digital stimuli.

The invention discloses a nano biomass-based flexible electrode as well as a preparation method and application thereof, and belongs to the technical field of biosensors. According to the invention, biomass is used for preparing the flexible electrode, and the biomass has the advantages of being natural, renewable, biodegradable, high in yield, low in cost and the like; the sensor prepared by taking the biomass-based flexible electrode as the substrate cannot cause environmental pollution, and the biomass has good biocompatibility, so that skin discomfort cannot be caused when the sensor prepared by taking the biomass-based flexible electrode as the substrate is worn. The sensor prepared by taking the nano biomass-based flexible electrode as a substrate has high air permeability and high sensitivity, and can promote rapid evaporation of sweat, so that the problem that the continuous monitoring precision is reduced due to sweat accumulation is solved.

The invention belongs to the technical field of biosensors, and provides a tetracycline ultra-sensitive detection method based on a CdTe (at) PVP (at) ZIF-8 core-shell material and a cascade amplification sensor. The sensor comprises an Apt-T composite probe, an M-2P chain, a complementary chain C, a self-locking DNAzyme hairpin chain and a hairpin chain H, the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the nucleotide sequences of the Apt, the T chain, the template chain M, the enriched chain P, the complementary chain C, the DNAzyme chain and the H chain are as shown in SEQ ID NO: 1-7. The sensor disclosed by the invention has the advantages of broad-spectrum recognition, high specificity, high detection speed, simplicity in operation, low detection limit and the like, can make up for the defects and deficiencies of an existing detection method for tetracycline antibiotics, realizes rapid and accurate ultra-sensitive detection on the tetracycline antibiotics, and has potential application prospects in food safety and environment detection.

A physiological signal monitoring device includes a base, a biosensor mounted to the base, a transmitter, and a sealing unit. The base is adapted to be mounted to a skin surface of a host. The biosensor includes a mounting seat and a sensing member that is mounted to the mounting seat. The sensing member is adapted to be partially inserted underneath the skin surface of the host for measuring an analyte of the host and to send a corresponding physiological signal. The transmitter is for receiving and transmitting the physiological signal, and has a bottom portion. The transmitter covers the base while the bottom portion faces the base. The sensing member is coupled to the transmitter. The sealing unit is used to seal paths through which a liquid possibly penetrates into an interior of the physiological signal monitoring device so as to avoid damage of the device.

The invention discloses a biosensor responding to glutaric acid concentration, and belongs to the technical field of biochemical engineering. According to the invention, regulatory protein and promoter elements are constructed on PSC101 series plasmids to obtain the biosensor responding to glutaric acid concentration. By performing point mutation on a-10 region and a-35 region of the response promoter PgcdH and optimizing expression of regulatory protein through the promoter and RBS engineering, the response sensitivity is improved, the response range is widened, and the application prospect in the field of glutaric acid high-yield strain screening is very wide.

A system includes a wire-holding fixture configured to be received in an EP station. The wire-holding fixture comprises terminals, and each terminal is electrically coupled to a respective working wire of a plurality of working wires. The working wires extend away from the wire-holding fixture. A set of EP stations is included, and each EP station is configured to receive the wire-holding fixture. A container is configured to hold an electropolymerization solution and positioned beneath the EP station such that the working wires is immersible. A board comprising a plurality of electrical contacts is included, and each electrical contact is configured to electrically engage a corresponding terminal. A controller is configured to apply a voltage to the working wires through the respective terminals and electrical contacts to initiate electropolymerization of the material from the electropolymerization solution to form a polymer layer on each working wire.

The invention relates to the technical field of online detection and analysis, and discloses a bone joint synovial fluid detection and analysis system based on microfluidics, which specifically comprises the following steps: acquiring synovial fluid rheological data and synovial fluid molecular activity data in a bone joint movement process and cartilage surface morphological data in a joint buckling process through a biosensor; constructing a synovial fluid dynamic anomaly analysis model, and performing synovial fluid function anomaly analysis through the synovial fluid rheological data and the molecular activity data; a cartilage stress anomaly analysis model is constructed, and cartilage stress anomaly analysis is carried out through cartilage surface morphological change data; obtaining a synovial fluid function abnormality analysis result and a cartilage stress abnormality analysis result to carry out bone joint function adaptability analysis; and acquiring a bone joint function adaptability analysis result and carrying out synovial fluid function abnormality analysis early warning. The problem that in the prior art, due to the fact that the bionic degree of a detection strategy is insufficient and bone joint dynamic parameters are deficient, the association degree of a static synovial fluid detection result and the actual joint rehabilitation condition of a patient is poor is solved.

The invention discloses a modular fluorescent RNA aptamer biosensor system, and relates to the field of medicine. Comprising a probe module, an enzyme system, a dye and a reaction buffer solution, the probe module comprises a promoter probe P and a reporter probe R, the enzyme system comprises SplintR ligase and T7RNA polymerase, and the reaction buffer solution comprises a transcription buffer solution and a SplintR ligase buffer solution. The promoter probe P comprises a T7 promoter sequence and a 5 '-phosphorylated upstream recognition region, and the length of the promoter probe P is 10nt. According to the present invention, the ligase-assisted probe assembly and the T7RNA polymerase-mediated fluorescent RNA aptamer transcription are integrated, the simultaneous detection of the multiple circRNA can be achieved without the complex probe labeling, the femtomole-level sensitivity and the excellent single base mutation distinguishing ability in the complex sample are provided, and the real-time fluorescence instrument and the portable device are adapted.

The invention is suitable for the technical field of man-machine interaction, and provides a control method and system of a tactile stimulation device and the tactile stimulation device.The method comprises the steps that a preset session program containing a plurality of stimulation block ordered sequences is loaded; real-time physiological data of the user are continuously collected through a biosensor, and the concentration degree state of the user is evaluated based on the real-time physiological data; when the evaluation result of the concentration degree state has a preset change, dynamically adjusting the execution logic of the session program; and generating a driving signal corresponding to the adjusted execution logic and outputting the driving signal to a driving motor to generate a tactile stimulus for adjusting the concentration of the user. According to the invention, the problem that the existing tactile stimulation equipment has a single stimulation mode, so that the user feels adaptive and the effect is reduced is solved.

The invention belongs to the field of antibiotic detection and biosensors, and particularly relates to a colorimetric array sensor for distinguishing different types of antibiotics based on platinum-based nano-enzyme and a detection method of the colorimetric array sensor. The colorimetric array sensor is composed of platinum nano-enzymes with three different structures, namely platinum nano-particles PtNPs, platinum nano-flowers PtNFs and gold-core platinum-shell nano-particles Au (at) PtNPs. According to the antibiotic detection method based on the platinum nano-enzyme colorimetric array sensing system, the peroxidase-like activity of the antibiotics is regulated and controlled through the non-specific interaction of the three platinum nano-enzymes (PtNPs, PtNFs and Au-coated PtNPs) and the antibiotics, a unique'fingerprint 'map is generated in combination with TMB-H2O2 chromogenic reaction, and efficient distinguishing and concentration detection of various antibiotics are achieved.

The invention provides a biocompatible selective outer membrane of an implantable biosensor and a preparation method of the biocompatible selective outer membrane, and the preparation method comprises the following steps: preparing an outer membrane diluent, and respectively dissolving a PS-P4VP copolymer, NH2-PEG-NH2 and PEGDGE in the outer membrane diluent to obtain a mixed solution; the ratio of PS to P4VP of the PS-P4VP copolymer is (0.5: 9.5)-(3: 7); coating the surface of an enzyme layer of the in-vivo implanted sensor with the mixed solution, and curing for 48-56 hours at normal temperature, so that an amino group of NH2-PEG-NH2 and a pyridine group of P4VP form a dynamic covalent bond to obtain an inner layer; the inner layer is immersed in deionized water for 1.5 h or above, the uncrosslinked PEG derivative is eluted, and a porous inner layer is obtained; and coating the surface of the porous inner layer with a polysulfobetaine solution to obtain the hydrophobic antifouling coating. By adopting the technical scheme, the mechanical strength and the long-term stability are improved.

The invention relates to the technical field of marine metering equipment, in particular to a marine organism sensor calibration jig and a marine organism sensor calibration method. The marine organism sensor calibration jig comprises a flow cell, and a connecting valve, a driving mechanism and an air blowing mechanism are sequentially arranged on the outer side of the flow cell from left to right; the connecting valve comprises an outer sleeve part, a rotating part arranged in the outer sleeve part, a liquid outlet pipe arranged on the outer wall of the rotating part and a rotating pipe arranged on the liquid outlet pipe in a sleeving mode. According to the calibration jig of the marine biological sensor and the calibration method of the calibration jig, movement of an inner protruding block in a wire groove can be accurately converted into 90-degree stepping rotation of a rotating pipe and a rotating valve, so that standard solution flow paths with different concentrations are sequentially switched, the uniqueness and self-locking performance of the position of a valve body after rotation are ensured through a ratchet and pawl mechanism, and the calibration accuracy of the marine biological sensor is improved. Positioning errors and delay caused by traditional manual or independent electric valve switching are effectively prevented, and the accuracy and repeatability of calibration data are improved.

A physiological signal monitoring device includes a base and a transmitter. The base is provided with a biosensor. The transmitter is removably coupled to the base, and includes a casing and an electrostatic-discharge protective unit. The casing has a socket for the biosensor to be removably inserted thereinto. The electrostatic-discharge protective unit is disposed to at least surround the periphery of the socket to dispel static electricity when electrostatic discharge occurs.