200 results about "Glucose oxidase" patented technology

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses a microneedle system for continuous blood glucose monitoring, which comprises a microneedle array, an electrode system, a circuit connection and signal acquisition unit and a fixing and packaging structure, the working electrode is used for penetrating into the skin and making contact with intertissue fluid to control the diffusion speed of glucose and prevent glucose oxidase from losing, the surface of the working electrode is wrapped with glucose oxidase cross-linked and fixed through glutaraldehyde, one end of the electrode system is inserted into the microneedle, and the other end of the electrode system is connected with the circuit connection and signal acquisition unit. The fixing and packaging structure is arranged on the outer side of the circuit connecting and signal collecting unit and used for wrapping the electronic device and fixing the microneedle system to the skin surface. According to the microneedle system for continuous blood glucose monitoring, rapid and accurate detection of in-vivo blood glucose level changes is achieved, and minimally invasive property and good patient compliance can be achieved while the detection sensitivity and accuracy are guaranteed.

The invention relates to the technical field of medical detection equipment, and particularly provides a wearable sweat glucose detection system which comprises a sweat collecting and conveying unit which is provided with a sweat inlet and a sweat flow channel which are communicated with each other and is used for collecting and conveying sweat secreted by skin; the electric signal acquisition unit is arranged on the sweat flow channel, comprises a counter electrode, a working electrode and a reference electrode which are sequentially arranged along the flowing direction of sweat in the sweat flow channel, and is used for acquiring a real-time electric signal related to the glucose concentration; the working electrode comprises a porous alloy structure layer composed of platinum and nickel and a glucose oxidase layer located on the surface of the porous alloy structure layer; the signal processing unit is used for acquiring the real-time glucose concentration according to the real-time electric signal and a preset change curve of the electric signal about the glucose concentration; the system can effectively improve the sensitivity of glucose concentration detection and reduce the dosage of precious metal.

The invention discloses a multi-enzyme compound noodle stretching agent and application thereof, and belongs to the technical field of food additives. The multi-enzyme compound noodle stretching agent is prepared from the following components in parts by mass: 1 to 10 parts of papain, 1 to 10 parts of trypsin, 1 to 10 parts of glucose oxidase, 1 to 10 parts of lipase and 10 to 30 parts of tea polyphenol. Through the synergistic effect of multienzyme compounding and tea polyphenol, the dough structure and the noodle processing performance are improved, the protein depolymerization and crosslinking degree and the protein oxidation degree of the dough can be regulated and controlled at the same time, the dough strength and ductility are considered, the cooking loss is reduced, meanwhile, the green and natural characteristics are kept, no chemical modifier is added, and the production cost is reduced. And the limitation of the existing single enzyme or chemical modifier is overcome, so that the method has a relatively good application prospect in the technical field of food additives.

The application discloses a kind of glucose oxidase sensors based on modified multi-walled carbon nanotube modification, it is related to glucose oxidase sensor technical field, including the following steps: (1) modified multi-walled carbon nanotube preparation;(2) glassy carbon electrode is carried out surface polishing treatment;(3) obtain modified multi-walled carbon nanotube dispersion;(4) obtain glucose oxidase liquid;(5) glucose glucose oxidase liquid impregnation electrode;(6) using Nafion solution covers electrode;The sensor obtained after the electrode is modified by introducing modified multi-walled carbon nanotube in the application, not only has higher glucose detection sensitivity, but also has excellent stability.

The invention relates to an external degradable microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: S1, preparing powdery CS-TA; s2, preparing a powdery Cu-TA nano enzyme; s3, adding the Cu-TA nano-enzyme and glucose oxidase GOD into water, stirring at 1-4 DEG C in an inert gas atmosphere, centrifuging, and re-suspending in water to obtain a GOD-coated Cu-TA compound enzyme solution; s4, adding CS-TA, hyaluronic acid, polyvinylpyrrolidone, L-arginine and a basic fibroblast growth factor into the GOD (at) Cu-TA compound enzyme solution, and preparing a microneedle mother solution; s5, preparing an aqueous solution containing CS-TA, hyaluronic acid and polyvinylpyrrolidone to obtain a substrate mother solution; and S6, filling a microneedle mold cavity of a mold with the microneedle mother solution to form enzyme series microneedles arranged in an array, then pouring a substrate mother solution to form a substrate, drying, and demolding to obtain the external degradable microneedle patch.

The present invention proposes a method and use for stabilizing a simple agglomerate for pH and salt and with adjustable size. In phosphoric acid buffer solutions with different salt concentrations and pH values, the polyacrylic acid hydroxypropyl group condensation body has specific stability; hydrophobic thermal response blocks are introduced into a hydroxypropyl acrylate monomer through reversible addition-fragmentation chain transfer polymerization to regulate and control the size of a condensation body, and a phase separation behavior is shown at different temperatures; a hydrophobic block without thermal response is introduced to regulate and control the size of the aggregate, and no thermal response behavior is shown. Continuous regulation and control of the size of the aggregate from the micron level to the nano level are achieved, and the requirements of different application scenes are met. The hydroxypropyl polyacrylate condensation body can be used for packaging various dyes and drugs; high-efficiency enzyme protection is achieved, formed aggregates can protect glucose oxidase, and the retention rate of enzyme activity reaches 85% or above after treatment is conducted for 3 hours at 60 DEG C; as a micro-reactor for the reaction of generating p-nitrobenzaldehyde phenylhydrazone from p-nitrobenzaldehyde and phenylhydrazine, the reaction rate is greatly improved.

The application discloses a new use of a fumaria officinalis extract and a pet food additive. The new use of the fumaria officinalis extract is used as a pet food additive for improving the first mouth preference of pet food and reducing the peculiar smell of pet feces, and the pet includes dogs and cats. The addition amount of the fumaria officinalis extract in the feed is 300 g / t; and the addition amount of glucose oxidase in the feed is 300 g / t. The addition in the daily ration can improve the abundance of probiotics in the intestinal tract of pets and reduce the abundance of pathogenic bacteria. The application can improve the first mouth preference of pets, dogs and cats, and the two have a certain synergistic effect, can effectively reduce the generation of fecal odor substances in the feces of the pets, dogs and cats, effectively improve the intestinal flora structure of the pets, dogs and cats, improve the intestinal health level of the pets, dogs and cats, improve the intestinal health condition of the pets, dogs and cats, and the product has the advantages of stable quality, high safety, simple preparation method and suitability for industrialized production.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

This invention relates to a dual-potential electrochemiluminescence immunosensor based on enzymatic in-situ generation of a co-reactant, its preparation method, and its application. The sensor includes: a substrate electrode; a GOx@Au@MoS2 multifunctional sensing interface; a hybrid probe consisting of a cathode probe and an anode probe; the cathode probe is a Py-HOF-labeled AFB1 antibody, and the anode probe is a UiO66-NH2@luminol-labeled OTA antibody; glucose is added to the detection solution, and hydrogen peroxide is generated in situ through immobilized glucose oxidase, serving as the single co-reactant for both the cathode and anode. During detection, this invention generates H2O2 in situ through enzymatic generation, simultaneously exciting ECL signals at both the cathode and anode; it solves problems such as poor stability of the H2O2 co-reactant, inconvenient concentration control, multiple operation steps, and insufficient continuous supply; and it enables simultaneous quantitative detection of AFB1 and OTA with a single sample addition, without the need for external H2O2.

The application provides a glucose detection test strip and a preparation method and application thereof. The preparation method of the glucose detection test strip comprises the following steps: taking copper sulfate pentahydrate, a phosphate buffer solution, glucose oxidase and horseradish peroxidase, mixing uniformly to obtain an enzyme-containing mixed solution; immersing a test strip in the enzyme-containing mixed solution, and obtaining an organic-inorganic hybrid nanoflower loaded test strip after reaction; immersing the organic-inorganic hybrid nanoflower loaded test strip in a mixed solution containing a chromogenic agent, a gel, bovine serum albumin and polyethylene glycol to obtain a glucose detection test strip. The preparation method of the glucose detection test strip has higher sensitivity and shorter response time.

This invention belongs to the technical field of filtration aids for food-grade enzyme preparation production, specifically relating to a composite filter aid comprising food-grade diatomaceous earth, food-grade expanded perlite, microcrystalline cellulose, food-grade silica powder, food-grade calcium carbonate, food-grade magnesium carbonate, hydroxypropyl methylcellulose, a diatomaceous earth porous framework compound modified with a low-adsorption sodium alginate pectin silica hydration layer for glucose oxidase, a perlite microcrystalline cellulose chitosan tannic acid-bridged gradient pore modification compound, and food-grade silica. Its preparation includes preparation of a basic mixture, preparation of a composite mixture, drying and cooling, and sieving. This invention forms a loose and stable filter cake, reduces filtration resistance, and improves the filtration speed, filtrate clarity, and enzyme activity retention rate of the glucose oxidase production solution.

Provided are a compound and method for improving the activity of glucose oxidase, a reagent, a biosensor, and a use of the compound. A sulfonyl sweetener and glucose oxidase are compounded, and a better compounding ratio is provided, so that the enzyme activity of glucose oxidase, the affinity of glucose oxidase to glucose and the catalytic efficiency of glucose oxidase for glucose in unit time can be significantly improved, and therefore, it is expected that the application effect of glucose oxidase in the fields of biosensors, food industry, medicines and the like can be improved.

The invention belongs to the technical field of biomedical materials. The invention provides a composite nano preparation for treating diabetic ulcer and promoting wound healing and a preparation method thereof. The preparation method comprises the following steps: mixing tetra (4-carboxyl phenyl) porphyrin and FeCl3. 6H2O for reaction, then adding a mixed solution composed of benzoic acid and 3, 5-dicarboxyphenylboronic acid for reaction, and then performing centrifugal drying; dispersing in water, adding a chloroplatinic acid solution, stirring, adding a sodium borohydride solution, continuously stirring, and centrifugally drying; stirring with a mixture of curcumin, flavonoid glycoside and astragaloside in ethanol, and carrying out centrifugal drying; and finally, stirring with glucose oxidase in water, and centrifugally drying. The composite nano preparation provided by the invention has an excellent antibacterial effect on multi-drug-resistant bacteria, can effectively destroy a biological membrane, can well treat diabetic ulcer and promote wound healing, and has a relatively good application prospect.

The invention provides a preparation method of a product for removing toxin in an animal body. The product for removing toxin in the animal body is prepared from the following components in parts by weight: 20kg of dandelion roots, 20kg of herba houttuyniae, 20kg of herba portulacae, 20kg of liquorice roots, 20-50kg of porcellanite, 10-20kg of glucose oxidase and 10-20g of beta-cyclodextrin. The preparation method comprises the following steps: extracting volatile oil from a herba houttuyniae medicinal material through a supercritical CO2 extraction method, stirring and mixing the volatile oil and beta-cyclodextrine to obtain an inclusion compound, drying the medicinal material after extraction, crushing the medicinal material together with other medicinal materials, sieving the crushed medicinal material together with other materials through a 30-mesh sieve in a dust-free feeding station, feeding into a mixing tank through vacuum feeding, and uniformly mixing to obtain a finished product. And sub-packaging by a full-automatic packaging machine to obtain the product. The product provided by the invention has the advantages of high bioavailability and convenient raw material source, and has the effect of removing toxins in animal bodies.

The invention discloses in-situ drug-loaded synergistic treatment gel P-GFe-coated Gel as well as a preparation method and application thereof, and belongs to the field of biological medicines. By integrating the photo-thermal characteristics of mesoporous polydopamine (MPDA), the CDT efficiency of Fe, the TME regulation effect of glucose oxidase (GOx) and the in-situ delivery capacity of protein hydrogel, PTT / CDT synergistic treatment is achieved, and the problems that in existing tumor treatment, targeting is insufficient, the curative effect is limited by TME, and systemic toxicity is high are solved. The preparation technology is simple and mild, raw materials are easy to obtain, large-scale production can be achieved, and wide application prospects are achieved in clinical treatment of malignant tumors such as breast cancer.

The present application relates to the technical field of biosensor, especially to a glucose sensor based on composite film and a preparation method thereof, after fixing layered MoS2 semiconductor material on a substrate, the present application continues to prepare ion gel film on the substrate, then modifies glucose oxidase on the surface of the ion gel film, after incubation and fixation, modifies a layer of Nafion film on the surface of the ion gel film, because glucose oxidase oxidizes and decomposes glucose to generate protons, with the selective proton passing effect of the Nafion film, the protons pass through the Nafion film to the porous network structure formed by the ion gel film and the layered MoS2 semiconductor material, and cooperate with the cations in the ion gel film to attract more carriers, and form a double-layer structure on the surface of MoS2, cooperate with the current multiplication effect, improve the transmission efficiency of the carriers, and improve the sensitivity of the sensor.

The present application belongs to the technical field of tobacco essence preparation and biocatalysis application, and particularly relates to a tobacco essence prepared based on composite enzymes and a preparation process. The process first proportionally compounds pectinase, beta-glucosidase, lipase, lipoxygenase, glucose oxidase and acetyltransferase, and forms microcapsules through a sodium alginate-chitosan system; prepares an extract after steam explosion pretreatment of tobacco powder, loquat leaves and monk fruit; adds the microcapsule enzymes for three-stage segmented enzymolysis; further adds hydroxypropyl-beta-cyclodextrin to include aroma molecules, and after enzyme inactivation and filtration, concentrates and adds vitamin E to stabilize the quality of the essence, so as to finally obtain a tobacco essence with rich aroma levels and good thermal stability. Through the synergistic effect of composite enzymes and multi-stage reaction regulation, the present application realizes the directional release and construction of flavor substances in natural raw materials, and the obtained essence is suitable for various application scenarios such as cigarette flavoring and paper spraying.

The invention belongs to the technical field of hydrogel preparation, and relates to a preparation method and application of metabolism programming hydrogel, and the preparation method comprises the following steps: 1, oxidizing dextran Dex through sodium periodate NaIO4 to generate oxidized dextran ODex rich in aldehyde; 2, under the protection of nitrogen, carrying out amino dynamic Schiff base reaction of APTC and carrying out covalent binding with an aldehyde group of oxidized dextran ODex to form ODex-APTC; 3, mixing ODex-APTCC and CMC GOx solutions at room temperature to form hydrogel based on hemiacetal crosslinking and a hydrogen bond mechanism; according to the invention, autonomous'antibacterial repair 'dual-phase switching is realized through metabolic microenvironment reprogramming; in the stage of wound surface high-glucose infection, the hydrogel utilizes a diabetes wound surface high-glucose environment as an endogenous fuel, and glucose reduction, active oxygen mediated sterilization and microenvironment acidification are realized at the same time through reaction of glucose oxidase and glucose; when glucose oxidase reacts, the acid environment triggers a controlled H2S release program, and wound healing is accelerated by relieving oxidative stress and activating a regeneration signal channel.

The invention belongs to the technical field of biological medicine, and discloses a protein nanomotor based on disulfide death and a preparation method and application thereof. The invention discloses a protein nanomotor, which comprises cystine or pharmaceutically acceptable salts thereof, and an enzyme connected with the cystine or the pharmaceutically acceptable salts thereof, the enzyme comprises glucose oxidase and an enzyme with a matrix degradation function, and the protein nano motor provided by the invention can trigger disulfide death of tumor (such as lung cancer) cells, so that a cell actin skeleton generates a large number of disulfide bonds and seriously shrinks, and finally, the cell structure comprehensively collapses and dies. Therefore, efficient killing of tumors is realized. Through synergistic effects of microenvironment remodeling, self-driven intra-tumor penetration promotion and disulfur death induction, the tumor treatment effect is significantly enhanced.

The invention discloses a preparation method of glucosyl puerarin, which belongs to the technical field of pharmaceutical chemicals, and comprises the following steps: S1, adding sugar for reaction, mixing puerarin with pure water, adjusting the pH value to 4.5-5.5, adding beta-cyclodextrin, heating to 45-55 DEG C, adding cyclodextrin glucosyltransferase for reaction for 16-22 hours, then adjusting the pH value to 0.1-0.8, adding activated carbon, filtering filtrate and staying overnight at low temperature; s2, desugaring reaction: adjusting the pH value of the filtrate to 3.8-4.5, heating to 55-65 DEG C, adding saccharifying enzyme and glucose oxidase to react for 25-40 minutes, adjusting the pH value to 0.5-1.2, and filtering; s3, column purification: enabling a reaction solution to pass through a mixed column containing sulfonated modified XAD-16 and amino modified HPD-720 resin, and performing graded elution to collect an ethanol eluent; s4, concentrating and drying to obtain a product. The problems that the water solubility of puerarin is poor, the purity of glucosyl puerarin is low, and impurities are difficult to remove can be solved.

This invention relates to a self-triggered demulsification Pickering emulsion cascade catalytic system, its preparation method, and its application. The preparation method includes: preparing Fe3O4 magnetic nanoparticles by co-precipitation; coating their surface with a SiO2 shell using a modified Stöber method to obtain Fe3O4@SiO2 core-shell nanoparticles; simultaneously grafting a triamine silane coupling agent and octyltrimethoxysilane onto the Fe3O4@SiO2 surface using a one-pot method to obtain magnetic amphiphilic nanoparticles; dividing the magnetic amphiphilic nanoparticles into two batches, and immobilizing glucose oxidase and Candida antarcticis lipase B respectively via EDC / NHS-mediated amide coupling reaction to obtain nanoparticle catalysts; using the nanoparticle catalysts as solid emulsifiers in Pickering emulsions to construct an oil-water two-phase cascade catalytic system, realizing a cascade catalytic reaction of glucose oxidation – peracidification – olefin epoxidation; utilizing the accumulation of gluconic acid during the reaction to lower the pH, triggering emulsion demulsification, achieving product separation and magnetic recovery of the nanoparticle catalysts.

The application relates to the field of slow-release fertilizers, and particularly discloses a nano-selenium drought-resistant and high-temperature-resistant slow-release fertilizer and a preparation method thereof. 0 The method comprises the following steps: fixing glucose oxidase on the surface of TiO2 nanoparticles, and performing cascade reaction of the glucose oxidase, glucose and sodium selenite under mild conditions to realize synchronous synthesis of Se The method is green, mild and controllable, the obtained selenium fertilizer has excellent stability and slow-release performance, can significantly improve the selenium content and yield of crops, and is suitable for selenium-rich functional agriculture.

A method for preparing an MOF-based glucose detection system, wherein a Zeolitic Imidazolate Framework (ZIF) is used as a metal-organic framework, and glucose oxidase (GOx) is loaded in the zeolitic imidazolate framework. The preparation method comprises the following steps: S1: adding water to a reaction flask in advance, and then sequentially adding a metal ion, an enzyme and an organic ligand in a molar ratio of 100-1500:1-10:1-150, and 0.1-0.5% (by weight) of ammonia water to the reaction flask, S2: mixing and reacting same at room temperature for more than 6 hours, and S3: after centrifugation by means of a centrifuge, removing the supernatant to obtain a solid precipitate of the Gox-MOF material loaded with glucose oxidase. A sensor for detecting trace glucose concentration in saliva, and an oral glucose meter comprising the sensor.

The invention provides a biological Fenton coupled heterotrophic nitrification-aerobic denitrifying bacterium NMP-containing wastewater treatment method, which comprises the following steps: S1, putting immobilized glucose oxidase and glucose into NMP-containing wastewater to react, and recovering the immobilized glucose oxidase after the reaction is finished to obtain H2O2-containing first wastewater; s2, adding Fe < 2 + > into the first wastewater for reaction to obtain second wastewater; and S3, putting the heterotrophic nitrification-aerobic denitrification bacteria into the second wastewater for reaction.

This invention relates to a glucose-responsive antibacterial nanocomposite microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. The glucose-responsive antibacterial nanocomposite microneedle patch is an integral soluble microneedle. The functional component of the microneedle material is a nanocomposite CIP / GOx@ZIF-8, which uses ZIF-8 as a nanocarrier, co-loading ciprofloxacin hydrochloride and glucose oxidase. This invention synthesizes CIP / GOx@ZIF-8 using a one-step self-assembly method, achieving co-loading of CIP HCl and GOx. Furthermore, this invention further prepares CIP / GOx@ZIF-8 into microneedles. The preparation method is simple, easy to use, and allows for self-administration. Topical application to skin wounds is safer and more effective than systemic administration, further improving drug delivery efficiency and delivering therapeutic agents deep into the wound, promoting wound healing. This invention combines multiple therapeutic mechanisms, significantly enhancing the antibacterial properties of the microneedles and achieving the treatment of bacterially infected diabetic wounds.

The invention discloses a DNA framework nucleic acid cascade enzyme, a paper-based colorimetric sensor and a preparation method and application of the paper-based colorimetric sensor, and belongs to the technical field of biological detection.The DNA framework nucleic acid cascade enzyme comprises a first enzyme, a second enzyme, a first DNA tetrahedron and a second DNA tetrahedron; the first enzyme is glucose oxidase with the surface modified by DBCO-pri, and the second enzyme is horse radish peroxidase with the surface modified by DBCO-pri; the first enzyme and the first DNA tetrahedron as well as the second enzyme and the second DNA tetrahedron are respectively connected through base complementary pairing; the first DNA tetrahedron and the second DNA tetrahedron are hybridized through a nucleic acid connecting chain to form a dimer structure. According to the invention, the DNA nanostructure is used for accurately fixing enzyme molecules and controlling the spatial arrangement of the enzyme molecules, the cascade catalytic efficiency is improved, and the advantages of low cost and portability of a paper-based platform are combined, so that high-sensitivity, rapid and visual detection of sweat glucose is realized.

The application discloses an electrochemical biosensor based on specific nucleic acid aptamer fragments and glucose oxidase. The specific recognition of the same target object by the double nucleic acid aptamer is utilized to combine the modified metal organic framework compound and the magnetic nanoparticles with the target object to form a self-assembly structure, and the magnetic nanoparticles are separated out by utilizing the magnetism of the magnetic nanoparticles. Meanwhile, the specific catalytic action of the glucose oxidase on glucose produces hydrogen peroxide to further oxidize 3,3'-diamino benzidine to generate an electrochemical signal, and the metal organic framework compound and the magnetic nanoparticles both have peroxidase-like activity, which can further synergistically catalyze the oxidation of 3,3'-diamino benzidine by hydrogen peroxide, and the sensitivity is improved. Thus, based on the linear relationship between different concentrations of pathogen solutions and corresponding electrochemical signal intensities, the electrochemical biosensor realizes high-sensitivity detection of the hyacinth yellow rot fungus.

The application discloses a glucose-responsive insulin delivery system and a preparation method and application thereof, and belongs to the technical field of biomaterials. The glucose-responsive insulin delivery system provided by the application comprises a delivery carrier and a bioactive component encapsulated in the carrier; the delivery carrier is a metal polyphenol network formed by a coordination reaction between epigallocatechin gallate and metal ions; and the bioactive component comprises glucose oxidase, catalase and insulin. The glucose-responsive insulin delivery system can adjust the release of insulin according to the concentration of glucose in the surrounding environment, so that the blood glucose can be maintained at a normal level.

The application provides a starch compound and a preparation process and application in food, and belongs to the technical field of baked food.The starch compound comprises hydroxypropyl distarch phosphate, oxidized starch, an emulsifier, a complex polysaccharide, wheat flour and white granulated sugar; the oxidized starch is obtained after step-by-step enzymolysis of glucose oxidase and laccase and oxidation treatment by using an oxidizing agent.The starch compound significantly improves the water holding property, beating resistance and baking stability of batter by compounding of the components, effectively inhibits the collapse of baked food in the freezing-thawing process, improves the appearance regularity and softness and delicacy of the product, and enhances the loading and retention capacity of flavor substances.The application of the starch compound in baked food is particularly suitable for frozen and room-temperature baked food, and provides an excellent raw material solution for high-quality and high-stability baked products.