488 results about "Glucose oxidase" patented technology

The present disclosure provides a preparation method for a light-colored water-soluble vegetable protein. The method comprises: adding protease and glucose oxidase into a vegetable protein solution for enzymolysis to obtain a water-soluble vegetable protein. The method effectively reduces browning reactions in the enzymolysis process, not only lightening the color, but also ensuring that the yield of polypeptide products remains undiminished.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention discloses a sugar response hydrogel wound dressing based on Prussian blue nano-enzyme and bacterial cellulose as well as a preparation method and application of the sugar response hydrogel wound dressing, and belongs to the field of medical dressings and biological materials. The sugar-responsive hydrogel wound dressing is good in mechanical property, and can be subjected to cascade reaction to play antibacterial and anti-inflammatory roles. The preparation method comprises the following steps: preparing Prussian blue nano-enzyme (PBNPs) by adopting a one-pot method and utilizing a self-polymerization reaction, and preparing a Prussian blue nano-enzyme bacterial cellulose compound by utilizing an isotonic adsorption method; glucose oxidase (GOx) is loaded on BC coated PBNPs by adopting an in-situ polymerization method, the sugar response hydrogel wound dressing based on PBNPs and BC is obtained, the preparation method is simple in process, and the obtained sugar response hydrogel wound dressing is good in stability, suitable for factory production and hopeful to be applied to diabetes wound treatment. Meanwhile, the PBNPs are applied to the field of diabetic wound dressings, and the application direction of the PBNPs is widened.

The invention belongs to the technical field of biomedical materials, and relates to a composite dressing with sequential response and functional synergy as well as preparation and application thereof, the composite dressing is of a double-layer composite structure, and comprises a PVA-GOx-MnO2 hydrogel layer and a temperature-sensitive micro-nanofiber membrane layer; the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into a polyvinyl alcohol solution under the condition of ice bath, and the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into the polyvinyl alcohol solution; the preparation method comprises the following steps: placing the temperature-sensitive micro-nano fiber membrane on a PVA-GOx-MnO2 hydrogel precursor solution or immersing the temperature-sensitive micro-nano fiber membrane in the PVA-GOx-MnO2 hydrogel precursor solution, carrying out crosslinking treatment, and demolding to obtain the composite dressing. The application is as follows: the material is used as a diabetic chronic wound repair material. The problems that an existing diabetes wound dressing is weak in microenvironment regulation and control capacity and the drug release time is not matched are solved; the preparation method is simple; the healing efficiency and the treatment safety of diabetic wounds are remarkably improved during application.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses a glucose electrochemical sensor based on protein passivated graphene as well as a preparation method and application of the glucose electrochemical sensor. The glucose electrochemical sensor comprises a glass substrate, a working electrode, a reference electrode, a counter electrode, Ag / AgCl ink coated on the surface of the reference electrode, and a composite structure formed on the surface of the working electrode, and the composite structure is composed of Prussian blue, graphene, a protein film and glucose oxidase which are sequentially modified on the surface of the working electrode. According to the present invention, the Prussian blue, the graphene, the protein film and the glucose oxidase are sequentially modified on the surface of the working electrode to construct the glucose electrochemical sensor based on the protein passivated graphene, and the glucose electrochemical sensor has characteristics of good specificity, good reproducibility and good long-term stability, therefore, the method has a good application prospect in glucose detection.

The invention discloses a hydrogel microneedle patch for noninvasive blood glucose detection as well as a preparation method and application of the hydrogel microneedle patch. According to the invention, the microneedle patch is prepared by a turnover technology and is noninvasively applied to the skin surface, so that simple and rapid extraction of interstitial fluid in vivo and a new method for real-time, rapid and quantitative detection of blood glucose in vivo are realized. The hydrogel microneedle patch contains two hydrogel components: one hydrogel component is hydrogel which can be cured and formed by ultraviolet light, and the other hydrogel component is hydrogel which can be cured and formed by ultraviolet light; and the other hydrogel is functional hydrogel with pH responsiveness, and glucose oxidase is added, so that the hydrogel can sensitively react to in-vivo glucose. The double-response hydrogel is jointly mixed and prepared into a microneedle patch form, the microneedle patch is endowed with noninvasive / minimally invasive property when applied to the skin surface, the microneedle patch is very suitable for extraction of interstitial fluid in vivo, and then real-time, rapid and accurate detection of the blood glucose level in vivo is achieved.

The invention discloses an antibacterial and antithrombotic medical catheter and a preparation method thereof. According to the medical catheter, a hydrogel coating is prepared on the surface of the medical catheter, the hydrogel coating contains a three-layer structure, and the medical catheter has low swelling property and excellent mechanical property, stability, durability, platelet adhesion resistance, antibacterial property and biocompatibility. The preparation method disclosed by the invention is simple, efficient and environment-friendly; the dopamine layer provides strong adhesion performance, glucose oxidase entrapped in FDA provides longer-term antibacterial performance, and finally the carboxylic acid betaine zwitterionic coating is introduced to further enhance the antithrombotic performance of the hydrogel coating, so that the hydrogel coating medical catheter is produced. The invention provides a new strategy for the low-expansion amphiphilic ion hydrogel coating with antithrombotic and antibacterial properties in the field of biomedical materials, especially as a medical catheter, and the coating can be applied to biomedical equipment in contact with blood.

The invention discloses a terahertz metamaterial sensing device and a blood glucose concentration detection method, and belongs to the field of terahertz science and technology. The terahertz metamaterial sensing device comprises an upper-layer asymmetric arc-shaped silicon column array and a lower-layer silicon substrate; the asymmetric arc-shaped silicon column array is composed of a plurality of arc-shaped silicon column unit structures with one long arc-shaped silicon column unit structure and one short arc-shaped silicon column unit structure. The long arc-shaped silicon column and the short arc-shaped silicon column have the same circle center and radius, and the arc length of the long arc-shaped silicon column is longer than that of the short arc-shaped silicon column; 3-aminopropyltriethoxysilane and glucose oxidase are used for carrying out surface functional modification on the asymmetric arc-shaped silicon column array and the silicon substrate. The device can be used for sensing and detecting blood glucose in a clinical serum sample, the concentration information of the blood glucose in the sample is obtained through the frequency shift of the resonance peak of the device, and the real-time, label-free and nondestructive detection of the sample is realized while the detection sensitivity is improved.

The invention discloses a preparation method and application of an iron-based covalent organic framework enzyme-loaded biological cascade catalytic material, and belongs to the field of materials. An iron-based covalent organic framework material is prepared by adopting a one-pot method, and glucose oxidase is further loaded, so that the biological cascade catalytic material for glucose detection is prepared. The biological cascade catalytic material obtained by the preparation method disclosed by the invention combines the advantages of nano enzyme and natural enzyme, and has excellent catalytic efficiency and long catalytic life; the Fe-COFs prepared by the method disclosed by the invention is strong in peroxidase-like activity and high in enzyme loading capacity, can adapt to a cascade system loaded by various enzymes, and widens the application prospect of constructing a biological cascade catalytic system based on COFs loaded natural enzymes.

The invention discloses a microneedle system for continuous blood glucose monitoring, which comprises a microneedle array, an electrode system, a circuit connection and signal acquisition unit and a fixing and packaging structure, the working electrode is used for penetrating into the skin and making contact with intertissue fluid to control the diffusion speed of glucose and prevent glucose oxidase from losing, the surface of the working electrode is wrapped with glucose oxidase cross-linked and fixed through glutaraldehyde, one end of the electrode system is inserted into the microneedle, and the other end of the electrode system is connected with the circuit connection and signal acquisition unit. The fixing and packaging structure is arranged on the outer side of the circuit connecting and signal collecting unit and used for wrapping the electronic device and fixing the microneedle system to the skin surface. According to the microneedle system for continuous blood glucose monitoring, rapid and accurate detection of in-vivo blood glucose level changes is achieved, and minimally invasive property and good patient compliance can be achieved while the detection sensitivity and accuracy are guaranteed.

The invention provides an immunoassay method based on instant synthesis of a nano-enzyme of a bimetallic CuCo Prussian blue analogue (CuCo PBA), and belongs to the field of chemical and biological sensing. According to the method, a sandwich type immune mode is formed by a glucose oxidase labeled antibody, a target object and an immobilized antibody, so that the concentration of the target object is in direct proportion to the amount of glucose oxidase. Hydrogen peroxide generated by catalyzing glucose by glucose oxidase catalyzes 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to oxidize and develop color under the assistance of CuCo PBA nano-enzyme (Cu < 2 + > and K3 [Co (CN) 6]) which is generated instantly (Cu < 2 + > and K3 [Co (CN) 6] are added instantly). And the color change of the detection liquid is recorded and is used for visual detection and quantitative detection by a microplate reader. The problems of advanced synthesis and storage of nano materials are avoided, the cost is low, the colorimetric detection method is simple in operation process, rapid in color development and high in sensitivity, and a novel method which is convenient and stable in performance is provided for clinical immunological detection.

The invention relates to glucose and pH dual-response hydrogel capable of intelligently responding to release of platelet-rich plasma, preparation and application, and belongs to the field of cellulose materials.Chitosan is alkalized and then subjected to carboxymethylation treatment, and carboxymethyl chitosan is obtained; the preparation method comprises the following steps: oxidizing nano cellulose to obtain hydroformylation modified nano cellulose; carboxymethyl chitosan and hydroformylation modified nano cellulose are mixed, glucose oxidase, platelet-rich plasma and nano cerium oxide particles are added in the mixing process, and the glucose and pH dual-response hydrogel capable of intelligently responding to release of platelet-rich plasma is obtained. According to the invention, a cross-linking agent is not needed, the operation is simple, the cost is low, the hydrogel can be rapidly formed, and the hydrogel can be automatically and gradually dissolved to release micromolecular substances wrapped in the hydrogel after being stimulated by glucose and pH change, is environment-friendly, has good biocompatibility, and is beneficial to promoting the application of the hydrogel in the fields of diabetes wound dressings and the like.

The invention relates to the technical field of glucose detection, in particular to a high-sensitivity glucose detection material and a preparation method thereof. The preparation raw materials of the high-sensitivity glucose detection material comprise the following components in parts by weight: 1-2 parts of modified glucose oxidase, 6-10 parts of modified porous microspheres, 1-1.5 parts of chloroplatinic acid and 0.6-1 part of palladium acetate. The prepared detection material is high in detection sensitivity and good in repeatability and stability.

The invention relates to a hydrogel light-operated multi-mode synergistic treatment platform based on a CuxS-MoS2 heterojunction for promoting wound healing of diabetes mellitus. And an interface induced electric field strategy is provided through density functional theory (DFT) calculation to optimize the charge migration behavior of the phototherapy agent. The multifunctional phototherapy system is constructed by taking CuxS-MoS2 with a built-in electric field as a core and PVA-CS hydrogel loaded with glucose oxidase (GOx) as a bracket. In a weakly acidic wound microenvironment, glucose at the wound is catalyzed by GOx to be converted into H2O2 and gluconic acid; meanwhile, the CuxS-MoS2 remarkably enhances the generation of reactive oxygen species (ROS) under illumination through peroxidase-like (POD) activity, photothermal effect (PTT) and heterojunction efficient carrier separation. After entering a proliferation period, the stent exerts catalase-like (CAT) activity to remove excessive ROS and release oxygen, and oxygen deficit and oxidative stress are relieved. In addition, the controllably released copper ions regulate and control the behavior of fibroblasts, promote healing and inhibit fibrosis. Experiments prove that the stent has excellent biocompatibility, effective antibacterial property and remarkable healing promoting effect in chronic wound repair of diabetes mellitus.

The invention discloses a compound probiotic fermented fish sauce and a preparation method thereof. The method comprises the following steps: cleaning fish and leftovers, adding distilled water, pulping to obtain fish cream, adding beta-D-glucose, glucose oxidase and catalase into the fish cream, and carrying out primary enzymolysis under stirring; adjusting the pH value of the primary enzymatic hydrolysate to be neutral, adding a compound enzyme to carry out secondary enzymatic hydrolysis, and carrying out enzyme deactivation to obtain a secondary enzymatic hydrolysate; adding table salt to obtain enzymatic hydrolysate; soybean meal, green beans, bran and water are mixed to obtain a koji material, the koji material is cooled to the room temperature after high-pressure sterilization, compound probiotics are inoculated for koji making, mold koji is added into the enzymatic hydrolysate for fermentation, electrodialysis desalination is conducted on fermentation liquor, then high-pressure sterilization is conducted, and the fish sauce is obtained. The fishy smell in the leftovers is removed by adding beta-D-glucose, glucose oxidase and catalase, and then the fishy smell is further degraded through fermentation; the green beans are added into the koji to prepare the koji, so that the fish sauce obtained by fermentation can keep specific fragrance, and the fish sauce product with good flavor and low fishy smell is obtained.

The invention relates to a MnO2 in-situ coated nano diamond drug, which is prepared by the following steps: by taking nano diamond as a carrier, modifying the surface of the nano diamond with polylysine so as to improve the dispersity and provide amino groups, then connecting the nano diamond with glucose oxidase rich in carboxyl groups through an amide reaction, and then coating the surface of the nano diamond with MnO2 through KMnO4 reduction so as to obtain the MnO2 in-situ coated nano diamond drug. Finally, the chemotherapeutic drug DOX is loaded through coordination and electrostatic interaction. According to the nano diamond medicine, in a subacid tumor microenvironment rich in GSH, the wrapped MnO2 shell is subjected to a redox reaction to generate Mn < 2 + >, meanwhile, the MnO2 nano structure can trigger H2O2 in the tumor microenvironment to be decomposed to generate O2, tumor hypoxia can be relieved, GOx can be exposed, glucose in a tumor area can be effectively catalyzed to generate H2O2 under the aerobic condition, and therefore the tumor hypoxia can be relieved. Glucose consumption effectively cuts off nutrition sources of tumor cells, inhibits tumor cell proliferation and realizes tumor hunger treatment. According to the invention, chemotherapy, hunger therapy and chemical kinetics therapy are cooperated to efficiently and selectively kill tumor cells, and a remarkable anti-tumor effect is achieved.

The invention discloses nanoparticles for synergistically treating tumors and a preparation method of the nanoparticles, and relates to the technical field of preparation of drugs containing inorganic active ingredients. The nanoparticles are composite nanoparticles Mo-PDA prepared by mixing and reacting inorganic active ingredients of phosphomolybdic acid Mo-POM and dopamine; the preparation method comprises the following steps: taking Mo-POM (phosphomolybdic acid), then loading Mo-PDA-coated GOx nanoparticles with a spherical structure formed by glucose oxidase (GOx), and carrying out mixing reaction by taking Mo-POM, adding deionized water, then adding dopamine, continuously stirring for 1 hour, and continuously reacting for 14-18 hours at the temperature of 150-170 DEG C. According to the preparation method disclosed by the invention, PDA is combined with Mo-containing polyacid salt and externally loaded with glucose oxidase (GOx), so that the PTT-modified CDT-hunger nanoparticle Mo-PDA-GOx for cooperatively treating tumors is synthesized, and the PTT-modified CDT-hunger nanoparticle Mo-PDA-GOx has excellent photo-thermal conversion efficiency which reaches 67.2%. The nanoparticles effectively exert synergistic treatment, and the treatment effect on tumors is improved.

The invention discloses a preparation method of an SERS (Surface Enhanced Raman Scattering) sensing microneedle patch and application of the SERS sensing microneedle patch in detection of trace blood sugar, and belongs to the technical field of material synthesis and biosensing. According to the preparation method of the SERS sensing microneedle patch disclosed by the invention, a surface enhanced Raman spectroscopy technology and a microneedle patch technology are integrated, a designed SERS probe takes urchin-shaped gold nanoparticles as a core, and a typical core-shell structure is formed through layer-by-layer self-assembly of polydopamine and gradual re-reduction of the gold nanoparticles; the Raman scattering signal is obviously enhanced. The upper layer of the double-layer structure microneedle is hyaluronic acid wrapping glucose oxidase, and the lower layer of the double-layer structure microneedle is methacryloyl hyaluronic acid wrapping an SERS probe. After glucose of interstitial fluid is collected by the microneedle, Raman spectrum characteristic fingerprint change of the SERS probe is caused through catalysis of glucose oxidase, non-invasive, rapid and high-sensitivity blood glucose detection is achieved, and the method is a more comfortable and convenient self-monitoring method.

The invention relates to the technical field of medical detection equipment, and particularly provides a wearable sweat glucose detection system which comprises a sweat collecting and conveying unit which is provided with a sweat inlet and a sweat flow channel which are communicated with each other and is used for collecting and conveying sweat secreted by skin; the electric signal acquisition unit is arranged on the sweat flow channel, comprises a counter electrode, a working electrode and a reference electrode which are sequentially arranged along the flowing direction of sweat in the sweat flow channel, and is used for acquiring a real-time electric signal related to the glucose concentration; the working electrode comprises a porous alloy structure layer composed of platinum and nickel and a glucose oxidase layer located on the surface of the porous alloy structure layer; the signal processing unit is used for acquiring the real-time glucose concentration according to the real-time electric signal and a preset change curve of the electric signal about the glucose concentration; the system can effectively improve the sensitivity of glucose concentration detection and reduce the dosage of precious metal.

The invention belongs to the technical field of CGM sensors, and particularly relates to a CGM electrode sensor and a preparation method thereof.The preparation method comprises the following steps that three sets of stainless steel microneedle arrays are prepared from a stainless steel substrate and serve as a counter electrode, a reference electrode and a working electrode respectively; mechanically polishing the stainless steel microneedle array, and performing composite pickling activation; after surface pretreatment, a sol-gel deposition aluminum oxide layer is adopted; preparing a gold plating solution, and plating gold on the stainless steel microneedle array deposited with the aluminum oxide layer by adopting two-stage pulse electroplating; the working electrode is prepared by PMMA coating, adhesion layer construction, glucose oxidase immobilization and limiting layer construction; the counter electrode, the reference electrode and the working electrode are respectively embedded into the substrate to form the microneedle array electrode sensor with a three-electrode system. Based on a nickel-free direct gold plating process, good uniformity, strong binding force and lower cost after gold plating can be realized, so that the stability of the continuous glucose sensor in use is improved.

The invention discloses a multi-enzyme compound noodle stretching agent and application thereof, and belongs to the technical field of food additives. The multi-enzyme compound noodle stretching agent is prepared from the following components in parts by mass: 1 to 10 parts of papain, 1 to 10 parts of trypsin, 1 to 10 parts of glucose oxidase, 1 to 10 parts of lipase and 10 to 30 parts of tea polyphenol. Through the synergistic effect of multienzyme compounding and tea polyphenol, the dough structure and the noodle processing performance are improved, the protein depolymerization and crosslinking degree and the protein oxidation degree of the dough can be regulated and controlled at the same time, the dough strength and ductility are considered, the cooking loss is reduced, meanwhile, the green and natural characteristics are kept, no chemical modifier is added, and the production cost is reduced. And the limitation of the existing single enzyme or chemical modifier is overcome, so that the method has a relatively good application prospect in the technical field of food additives.

The application discloses a kind of glucose oxidase sensors based on modified multi-walled carbon nanotube modification, it is related to glucose oxidase sensor technical field, including the following steps: (1) modified multi-walled carbon nanotube preparation;(2) glassy carbon electrode is carried out surface polishing treatment;(3) obtain modified multi-walled carbon nanotube dispersion;(4) obtain glucose oxidase liquid;(5) glucose glucose oxidase liquid impregnation electrode;(6) using Nafion solution covers electrode;The sensor obtained after the electrode is modified by introducing modified multi-walled carbon nanotube in the application, not only has higher glucose detection sensitivity, but also has excellent stability.

The invention relates to an external degradable microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: S1, preparing powdery CS-TA; s2, preparing a powdery Cu-TA nano enzyme; s3, adding the Cu-TA nano-enzyme and glucose oxidase GOD into water, stirring at 1-4 DEG C in an inert gas atmosphere, centrifuging, and re-suspending in water to obtain a GOD-coated Cu-TA compound enzyme solution; s4, adding CS-TA, hyaluronic acid, polyvinylpyrrolidone, L-arginine and a basic fibroblast growth factor into the GOD (at) Cu-TA compound enzyme solution, and preparing a microneedle mother solution; s5, preparing an aqueous solution containing CS-TA, hyaluronic acid and polyvinylpyrrolidone to obtain a substrate mother solution; and S6, filling a microneedle mold cavity of a mold with the microneedle mother solution to form enzyme series microneedles arranged in an array, then pouring a substrate mother solution to form a substrate, drying, and demolding to obtain the external degradable microneedle patch.

The present invention proposes a method and use for stabilizing a simple agglomerate for pH and salt and with adjustable size. In phosphoric acid buffer solutions with different salt concentrations and pH values, the polyacrylic acid hydroxypropyl group condensation body has specific stability; hydrophobic thermal response blocks are introduced into a hydroxypropyl acrylate monomer through reversible addition-fragmentation chain transfer polymerization to regulate and control the size of a condensation body, and a phase separation behavior is shown at different temperatures; a hydrophobic block without thermal response is introduced to regulate and control the size of the aggregate, and no thermal response behavior is shown. Continuous regulation and control of the size of the aggregate from the micron level to the nano level are achieved, and the requirements of different application scenes are met. The hydroxypropyl polyacrylate condensation body can be used for packaging various dyes and drugs; high-efficiency enzyme protection is achieved, formed aggregates can protect glucose oxidase, and the retention rate of enzyme activity reaches 85% or above after treatment is conducted for 3 hours at 60 DEG C; as a micro-reactor for the reaction of generating p-nitrobenzaldehyde phenylhydrazone from p-nitrobenzaldehyde and phenylhydrazine, the reaction rate is greatly improved.

The application discloses a new use of a fumaria officinalis extract and a pet food additive. The new use of the fumaria officinalis extract is used as a pet food additive for improving the first mouth preference of pet food and reducing the peculiar smell of pet feces, and the pet includes dogs and cats. The addition amount of the fumaria officinalis extract in the feed is 300 g / t; and the addition amount of glucose oxidase in the feed is 300 g / t. The addition in the daily ration can improve the abundance of probiotics in the intestinal tract of pets and reduce the abundance of pathogenic bacteria. The application can improve the first mouth preference of pets, dogs and cats, and the two have a certain synergistic effect, can effectively reduce the generation of fecal odor substances in the feces of the pets, dogs and cats, effectively improve the intestinal flora structure of the pets, dogs and cats, improve the intestinal health level of the pets, dogs and cats, improve the intestinal health condition of the pets, dogs and cats, and the product has the advantages of stable quality, high safety, simple preparation method and suitability for industrialized production.

The present disclosure presents glucose monitoring systems and methods. One such system comprises a glucose monitoring sensor having at least a counter electrode, a reference electrode, and a working electrode; and a silicon-carbide-silicon-carbide-nanoparticles-electrospun-nanofibrous-membrane contacting the working electrode. The system further includes a glucose oxidase enzyme solution contacting the silicon-carbide-silicon-carbide-nanoparticles-electrospun-nanofibrous-membrane, wherein a conductive polymer membrane assists in binding the glucose oxidase enzyme composition with the silicon-carbide-silicon-carbide-nanoparticles-electrospun-nanofibrous-membrane. Other systems and methods are also provided.

The invention discloses a difunctional nano preparation for tumor starvation and photothermal therapy as well as a preparation method and application of the difunctional nano preparation. The difunctional nano preparation is prepared from PDA (at) MXene and glucose oxidase loaded on the PDA (at) MXene, wherein the glucose oxidase is loaded on the PDA (at) MXene; the PDA (at) MXene comprises an MXene nanosheet and polydopamine, and the MXene nanosheet is modified by the polydopamine. According to the invention, polydopamine (PDA) is modified on an MXene nanosheet so as to improve the photothermal conversion efficiency, and glucose oxidase (GOX) is loaded on PDA-coated MXene with a large surface area so as to catalyze glucose in situ. The bifunctional nano preparation shows higher photo-thermal response efficiency than that of a single MXene nanosheet, promotes the pharmacokinetic effect of glucose oxidase, and realizes '1 + 1gt, multipurpose 'deep tumor ablation.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

This invention relates to a dual-potential electrochemiluminescence immunosensor based on enzymatic in-situ generation of a co-reactant, its preparation method, and its application. The sensor includes: a substrate electrode; a GOx@Au@MoS2 multifunctional sensing interface; a hybrid probe consisting of a cathode probe and an anode probe; the cathode probe is a Py-HOF-labeled AFB1 antibody, and the anode probe is a UiO66-NH2@luminol-labeled OTA antibody; glucose is added to the detection solution, and hydrogen peroxide is generated in situ through immobilized glucose oxidase, serving as the single co-reactant for both the cathode and anode. During detection, this invention generates H2O2 in situ through enzymatic generation, simultaneously exciting ECL signals at both the cathode and anode; it solves problems such as poor stability of the H2O2 co-reactant, inconvenient concentration control, multiple operation steps, and insufficient continuous supply; and it enables simultaneous quantitative detection of AFB1 and OTA with a single sample addition, without the need for external H2O2.