617 results about "Glucose oxidase" patented technology

The invention provides a thermal-stability glucose oxidase mutant, polynucleotide for encoding the glucose oxidase mutant, a recombinant expression vector containing the polynucleotide, a host cell for expressing the glucose oxidase mutant, and applications of the thermal-stability glucose oxidase mutant, the polynucleotide, the recombinant expression vector and the host cell in the fields of food, chemical industry, medicine, agriculture or feed.

The invention discloses a preparation method of an MOF glucose detection system, ZIF is used as a metal organic framework, glucose oxidase (GOx) is loaded in the ZIF, the preparation method comprises the following steps: S1, water is added into a reaction bottle in advance, then metal ions, enzyme and organic ligands with the molar ratio of (100-1500): (1-10): (1-150) are added into the reaction bottle in advance, and the reaction bottle is filled with water; the method comprises the following steps: S1, adding 0.1-0.5% (by weight) of ammonia water into a reaction bottle, S2, mixing and reacting at room temperature for more than 6 hours, and S3, centrifuging by a centrifugal machine, and removing supernate to obtain solid precipitate of the GOx-MOF material loaded with glucose oxidase. The invention also discloses an application of the MOF glucose detection system in saliva glucose content detection. The method can be applied to glucose detection of oral saliva, and even if the glucose content is extremely low, the glucose concentration in the saliva can be efficiently and accurately detected.

Hydrogel comprises hydroxyapatite nanowires and a base material for forming the hydrogel, and further comprises glucose oxidase (GOx), gallic acid, magnesium ions (Mg < 2 + >) and the like, the gallic acid and the Mg < 2 + > form Mg-MOF, and the GOx is loaded on the Mg-MOF, so that the diabetes microenvironment of a bone defect part is locally adjusted, and bone defect repair is facilitated. Through verification, the sustained-release gallic acid of GOx-MOF participates in cascade reaction, GOx is removed to generate H2O2, the benefit is maximized, and the sustained-release gallic acid has good effects of controlling blood glucose, removing ROS, regulating inflammation and promoting osteogenesis and angiogenesis. The research is expected to play a role in microenvironment regulation of diabetes mellitus bone regeneration and minimally invasive treatment of bone defects.

The invention discloses a glucose response type insulin delivery system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The glucose response type insulin delivery system provided by the invention comprises a delivery carrier and a bioactive component encapsulated in the carrier, the delivery carrier is a metal polyphenol network formed by carrying out coordination reaction on epigallocatechin gallate and metal ions; the bioactive components comprise glucose oxidase, catalase and insulin. The glucose response type insulin delivery system provided by the invention can adjust the release of insulin according to the concentration of glucose in the surrounding environment, so that the blood glucose is maintained at a normal level.

The invention relates to nanoparticle-loaded antibacterial composite hydrogel as well as a preparation method and application thereof, a hydrogel network main body is formed by grafting phenylboronic acid to methacrylated I-type collagen rCol1MA-PBA and methacrylated polylysine EPLMA and adding glucose oxidase GoX for crosslinking, and is loaded with core-shell nano-enzyme C (at) Z (at) T at the same time, and the core-shell nano-enzyme C (at) Z (at) T is loaded with the core-shell nano-enzyme C (at) Z (at) T and the core-shell nano-enzyme C (at) Z (at) T; the core-shell nano-enzyme C (at) Z (at) T is composed of a spherical CeO2 nano-particle core, a zeolite imidazate framework material TIF-8 shell and tannic acid TA coating the shell. The raw materials of the hydrogel are easy to obtain, the preparation method is simple, the price is low, and the components are safe and reliable; in addition, multiple functions of resisting bacteria, resisting inflammation, resisting oxidation, promoting angiogenesis and the like are integrated, a more comprehensive treatment effect is provided, and the dressing can be used for healing the wound surface of diabetes mellitus.

The invention provides double-crosslinking acellular matrix hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: (1) carrying out decellularization treatment on a porcine dermal layer to obtain a decellularized porcine dermal matrix, (2) putting freeze-dried powder of the decellularized porcine dermal matrix into an acidic pepsin solution, stirring and digesting, adjusting the concentration, adding 10xPBS, adjusting the pH value to obtain a pregel solution, and carrying out freeze-drying on the pregel solution to obtain the porcine dermal matrix freeze-dried powder. (3) putting gel formed by the pre-gel solution into a tannic acid solution for cross-linking to obtain mono-cross-linked acellular matrix hydrogel; (4) smashing the mono-cross-linked acellular matrix hydrogel, and mixing the smashed mono-cross-linked acellular matrix hydrogel with a glucose oxidase solution and a ferrous ion solution with the concentration of 0.1-10mg / ml to obtain a bi-cross-linked acellular matrix hydrogel precursor; 5, the double-crosslinking acellular matrix hydrogel precursor is placed under the condition of glucose with the concentration being 10-200 mg / dl to form the double-crosslinking acellular matrix hydrogel. The hydrogel prepared through the method can achieve the balance of safety, supporting performance and degradability, and is wide in application range.

The invention belongs to the technical field of biomedical materials, and discloses injectable heterogeneous cascade nano-enzyme antibacterial hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: in a process of preparing cerium dioxide nano-enzyme by a reverse micelle method, introducing molybdenum disulfide nano-particles with a nano flower-shaped structure so as to deposit the cerium dioxide nano-enzyme on the surfaces of the molybdenum disulfide nano-particles in situ to obtain nano-composite particles; through electrostatic interaction, glucose oxidase is loaded on the surfaces of the nano composite particles to form a heterogeneous cascade nano enzyme material; the preparation method comprises the following steps: uniformly mixing sodium alginate and hyaluronic acid in water, and sequentially adding an NO donor, a heterogeneous cascade nano-enzyme material and a cross-linking agent to obtain the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel. The preparation method is simple, and the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel has a strong antibacterial effect and can be used for preparing the antibacterial hydrogel. Particularly, the compound has a remarkable killing effect on gram-negative bacteria and gram-positive bacteria.

The invention discloses a microneedle patch for quantitatively detecting the blood sugar concentration of a human body as well as a preparation method and application of the microneedle patch, and belongs to the technical field of medicine detection. Mixing the PVA solution and the chitosan solution, adding the silk fibroin solution, and mixing to obtain a mixed solution; adding the mixed solution into a microneedle patch mold, enabling the mixed solution to enter the tip part of a microneedle, and performing vacuum filling on a cavity; adding a glucose oxidase solution and a potassium iodide solution into the mixed solution, mixing, continuously pouring the obtained mixed solution into a microneedle patch mold, filling a backing part at the upper end of a needle tip in the microneedle patch mold with the mixed solution, filling a cavity with the mixed solution in a vacuum manner, and repeatedly freezing, thawing and drying to obtain the microneedle patch for quantitatively detecting the blood glucose concentration of the human body. The microneedle patch for quantitatively detecting the blood sugar concentration of the human body can quantitatively detect the blood sugar of the human body, can visually reflect a detection result, is rapid and painless, low in cost, high in mechanical property and good in stability, and provides guiding significance for early diagnosis of diabetes mellitus.

The present disclosure provides a preparation method for a light-colored water-soluble vegetable protein. The method comprises: adding protease and glucose oxidase into a vegetable protein solution for enzymolysis to obtain a water-soluble vegetable protein. The method effectively reduces browning reactions in the enzymolysis process, not only lightening the color, but also ensuring that the yield of polypeptide products remains undiminished.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention discloses a method for realizing'active ' / controllable free radical polymerization of hydrophilic and / or hydrophobic monomers under an air condition, relates to the field of'active' / controllable free radical polymerization, and aims to solve the problem of narrow monomer selection range in the prior art. A functional monomer comprising a hydrophobic monomer, a halide, copper bromide, a ligand and a nonionic surfactant-GOx are subjected to monomer polymerization in a solvent through an initiator azodiisobutylimidazoline hydrochloride (VA-044), and a polymer with narrow target molecular weight and molecular weight distribution is formed. According to the method, the glucose oxidase modified by the nonionic surfactant is introduced into the'active ' / controllable free radical polymerization reaction, and an effective way for quickly and efficiently synthesizing a target polymer from a hydrophobic monomer under the mild air condition is provided.

The invention discloses a method for treating halogenated organic matter pollution in water by a Fenton-like system based on a pulsed electric field, which comprises the following steps: pre-adjusting the pH value of halogenated organic matter sewage to be treated to 5-8, then adding green rust, introducing pulse current after the system reaches a stable state, adding glucose and glucose oxidase in a stirring state to initiate a Fenton-like reaction, and carrying out a Fenton-like reaction to obtain a Fenton-like reaction solution; the treatment of halogenated organic pollution in water is realized; wherein the current density ranges from 75 mA / cm < 2 > to 125 mA / cm < 2 >, and the current density is increased in a gradient mode every 20-40 min. According to the invention, to-be-treated halogenated organic matter sewage is used as an electrolyte, under the influence of a pulsed electric field, a water body is electrolyzed by using current to generate ClO <-> with high oxidation performance for cooperative treatment, and low-potential Fe in the sewage and C generated by oxidizing glucose generate potential difference to form infinite tiny primary batteries, so that electrode reaction is generated; meanwhile, the pulsed electric field can improve more catalytic sites of glucose oxidase for a patina / glucose-glucose oxidase system, so that the overall catalytic effect is improved, and stable treatment of halogenated organic matter sewage is realized.

The invention discloses a sugar response hydrogel wound dressing based on Prussian blue nano-enzyme and bacterial cellulose as well as a preparation method and application of the sugar response hydrogel wound dressing, and belongs to the field of medical dressings and biological materials. The sugar-responsive hydrogel wound dressing is good in mechanical property, and can be subjected to cascade reaction to play antibacterial and anti-inflammatory roles. The preparation method comprises the following steps: preparing Prussian blue nano-enzyme (PBNPs) by adopting a one-pot method and utilizing a self-polymerization reaction, and preparing a Prussian blue nano-enzyme bacterial cellulose compound by utilizing an isotonic adsorption method; glucose oxidase (GOx) is loaded on BC coated PBNPs by adopting an in-situ polymerization method, the sugar response hydrogel wound dressing based on PBNPs and BC is obtained, the preparation method is simple in process, and the obtained sugar response hydrogel wound dressing is good in stability, suitable for factory production and hopeful to be applied to diabetes wound treatment. Meanwhile, the PBNPs are applied to the field of diabetic wound dressings, and the application direction of the PBNPs is widened.

The invention discloses a synthesis and purification method of electronic grade phosphorane, which relates to the technical field of phosphorane synthesis, and has the advantages that impurities such as metal oxides and carbides in a silicon carbide ceramic substrate are removed by selecting the silicon carbide ceramic substrate and performing high-temperature annealing treatment, so that the atomic arrangement of the substrate is more regular and ordered; compared with the prior art, the method has the advantages that the gallium phosphide crystal structure is more complete, so that the gallium phosphide crystal structure has higher structural stability, meanwhile, after impurities are removed, the interaction between the substrate and reactants in a high-temperature reaction environment is more controllable, the chemical stability is enhanced, a firm and reliable base is provided for growth of gallium phosphide crystals, and by adopting the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate, the yield of the gallium phosphide crystal is increased. The production of waste gas is reduced by utilizing the extremely low volatility of the gallium phosphide, and meanwhile, the reaction rate between hydrogen phosphide and hydrogen is accelerated by introducing glucose oxidase for catalytic reaction, so that the gallium phosphide crystal is promoted to efficiently and directionally grow on the silicon carbide ceramic substrate, and the product quality and the production efficiency are improved.

The invention discloses a glucose oxidase mutant with improved acid resistance and heat resistance as well as a construction method and application of the glucose oxidase mutant, and belongs to the technical field of bioengineering. According to the present invention, glucose oxidase AsGODP derived from Aspergillus scleroticus is adopted as a female parent, and Glu148 and Val179 are subjected to synchronous mutation to form Lys148 and Ala179 so as to obtain the mutant E148K / V179A; experiments show that the pH stability, the thermal stability, the half-life period at 75 DEG C and the catalytic performance of the mutant E148K / V179A are obviously improved compared with those of wild AsGODP. According to the catalytic performance, when the mutant E148K / V179A is applied to bread baking, the gluten degree of dough can be effectively improved under the fermentation condition, the bread texture is improved, and therefore the mutant E148K / V179A shows huge development potential in the bread baking industry.

The invention provides a nano enzyme, a preparation method and application thereof, and a glucose concentration detection method. The Ru / Co-N and Zn-CNCs nano-material is prepared by adopting a calcination method and a reduction method, the material can quickly catalyze the oxidation reaction of TMB in the presence of hydrogen peroxide, the cascade reaction colorimetric sensor for glucose detection is successfully constructed by combining with glucose oxidase, and the preparation method is simple and convenient to operate, economical and efficient; due to the synergistic effect generated by doping of Ru metal, the nano-enzyme has better catalytic activity, meanwhile, through combination with glucose oxidase, the system can achieve ultra-sensitive colorimetric detection of glucose, excellent stability and linear response characteristics are shown, and the detection accuracy is further improved. Through colorimetric detection, the method can sensitively detect glucose in a range of 10-2000 [mu] M, and the detection limit is as low as 0.61 [mu] M.

The invention belongs to the technical field of gene engineering, and discloses a glucose oxidase mutant as well as a preparation method and application thereof. Relative to parent glucose oxidase with an amino acid sequence as shown in SEQ ID NO: 1, the glucose oxidase mutant comprises a disulfide bond mutation site K37C + A572C. Compared with parent glucose oxidase, the thermal stability of the glucose oxidase mutant is obviously improved, good heat resistance is embodied, and production and application of gluconate are facilitated.

The invention belongs to the technical field of biomedical materials, and relates to a composite dressing with sequential response and functional synergy as well as preparation and application thereof, the composite dressing is of a double-layer composite structure, and comprises a PVA-GOx-MnO2 hydrogel layer and a temperature-sensitive micro-nanofiber membrane layer; the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into a polyvinyl alcohol solution under the condition of ice bath, and the PVA-GOx-MnO2 hydrogel precursor solution is prepared by adding glucose oxidase and MnO2 nano particles into the polyvinyl alcohol solution; the preparation method comprises the following steps: placing the temperature-sensitive micro-nano fiber membrane on a PVA-GOx-MnO2 hydrogel precursor solution or immersing the temperature-sensitive micro-nano fiber membrane in the PVA-GOx-MnO2 hydrogel precursor solution, carrying out crosslinking treatment, and demolding to obtain the composite dressing. The application is as follows: the material is used as a diabetic chronic wound repair material. The problems that an existing diabetes wound dressing is weak in microenvironment regulation and control capacity and the drug release time is not matched are solved; the preparation method is simple; the healing efficiency and the treatment safety of diabetic wounds are remarkably improved during application.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses a glucose electrochemical sensor based on protein passivated graphene as well as a preparation method and application of the glucose electrochemical sensor. The glucose electrochemical sensor comprises a glass substrate, a working electrode, a reference electrode, a counter electrode, Ag / AgCl ink coated on the surface of the reference electrode, and a composite structure formed on the surface of the working electrode, and the composite structure is composed of Prussian blue, graphene, a protein film and glucose oxidase which are sequentially modified on the surface of the working electrode. According to the present invention, the Prussian blue, the graphene, the protein film and the glucose oxidase are sequentially modified on the surface of the working electrode to construct the glucose electrochemical sensor based on the protein passivated graphene, and the glucose electrochemical sensor has characteristics of good specificity, good reproducibility and good long-term stability, therefore, the method has a good application prospect in glucose detection.

The invention relates to the technical field of sensors, and discloses a continuous glucose sensing device based on a microneedle array and a preparation method, the device comprises a base and a metal microneedle array, the base is a printed circuit board, conductive contacts are attached to the back surface of the base, and a metal through hole array is arranged on the base; the metal microneedle array is fixed in the metal through hole array from the front surface of the base; the adjacent metal microneedles are electrically connected, and the metal microneedles and the conductive contacts are electrically connected. The metal microneedle array is divided into a glucose sensing area and a differential sensing area, metal microneedles in the glucose sensing area and the differential sensing area are modified to comprise counter electrodes, working electrodes and reference electrodes, the working electrodes of the glucose sensing area are modified with glucose oxidase layers, and the reference electrodes of the glucose sensing area are modified with reference electrodes. And no glucose oxidase layer is arranged on the working electrode in the differential sensing area. By introducing the differential sensing electrode, common-mode interference signals caused by other electrochemical active substances can be effectively removed, and an accurate detection result is obtained.

The present invention relates to the field of glucose oxidase preparation, and discloses a glucose oxidase preparation device, including a salting-out chamber, wherein a mixed aluminum cylinder is movably mounted on the inner upper side of the salting-out chamber through a bearing seat, a central axis is movably mounted in the inner middle of the salting-out chamber, a stirring rod is fixedly connected to both sides of the middle of the central axis, a magnetic rod is fixedly mounted on both sides of the inner side of the salting-out chamber, a feeding chamber is fixedly connected to both sides of the inner side of the shell, and the bottom of the feeding chamber is connected to the inner side of the mixed aluminum cylinder. The present invention drives the stirring rod to rotate clockwise through the central axis, and the mixed aluminum cylinder rotates counterclockwise at the same time, and the two cooperate to fully mix the crude enzyme solution and the salting-out material, thereby improving the mixing effect, and utilizing the magnetic rods on both sides of the inner side of the salting-out chamber to generate an induced current and an electric field when the mixed aluminum cylinder rotates, so that the charged glucose oxidase molecules are accelerated to interact and precipitate with the salting-out agent under the action of the electric field, thereby improving the salting-out efficiency.

The invention discloses a hydrogel microneedle patch for noninvasive blood glucose detection as well as a preparation method and application of the hydrogel microneedle patch. According to the invention, the microneedle patch is prepared by a turnover technology and is noninvasively applied to the skin surface, so that simple and rapid extraction of interstitial fluid in vivo and a new method for real-time, rapid and quantitative detection of blood glucose in vivo are realized. The hydrogel microneedle patch contains two hydrogel components: one hydrogel component is hydrogel which can be cured and formed by ultraviolet light, and the other hydrogel component is hydrogel which can be cured and formed by ultraviolet light; and the other hydrogel is functional hydrogel with pH responsiveness, and glucose oxidase is added, so that the hydrogel can sensitively react to in-vivo glucose. The double-response hydrogel is jointly mixed and prepared into a microneedle patch form, the microneedle patch is endowed with noninvasive / minimally invasive property when applied to the skin surface, the microneedle patch is very suitable for extraction of interstitial fluid in vivo, and then real-time, rapid and accurate detection of the blood glucose level in vivo is achieved.

The invention discloses an antibacterial and antithrombotic medical catheter and a preparation method thereof. According to the medical catheter, a hydrogel coating is prepared on the surface of the medical catheter, the hydrogel coating contains a three-layer structure, and the medical catheter has low swelling property and excellent mechanical property, stability, durability, platelet adhesion resistance, antibacterial property and biocompatibility. The preparation method disclosed by the invention is simple, efficient and environment-friendly; the dopamine layer provides strong adhesion performance, glucose oxidase entrapped in FDA provides longer-term antibacterial performance, and finally the carboxylic acid betaine zwitterionic coating is introduced to further enhance the antithrombotic performance of the hydrogel coating, so that the hydrogel coating medical catheter is produced. The invention provides a new strategy for the low-expansion amphiphilic ion hydrogel coating with antithrombotic and antibacterial properties in the field of biomedical materials, especially as a medical catheter, and the coating can be applied to biomedical equipment in contact with blood.

The invention discloses a terahertz metamaterial sensing device and a blood glucose concentration detection method, and belongs to the field of terahertz science and technology. The terahertz metamaterial sensing device comprises an upper-layer asymmetric arc-shaped silicon column array and a lower-layer silicon substrate; the asymmetric arc-shaped silicon column array is composed of a plurality of arc-shaped silicon column unit structures with one long arc-shaped silicon column unit structure and one short arc-shaped silicon column unit structure. The long arc-shaped silicon column and the short arc-shaped silicon column have the same circle center and radius, and the arc length of the long arc-shaped silicon column is longer than that of the short arc-shaped silicon column; 3-aminopropyltriethoxysilane and glucose oxidase are used for carrying out surface functional modification on the asymmetric arc-shaped silicon column array and the silicon substrate. The device can be used for sensing and detecting blood glucose in a clinical serum sample, the concentration information of the blood glucose in the sample is obtained through the frequency shift of the resonance peak of the device, and the real-time, label-free and nondestructive detection of the sample is realized while the detection sensitivity is improved.

The invention discloses hydrogel based on a self-adaptive biological catalysis system, a preparation method and application of the hydrogel as a diabetes wound dressing, and the preparation method of the hydrogel comprises the following steps: weighing oxidized sodium alginate, adding the oxidized sodium alginate into ultrapure water for dissolving, adding gelatin, glucose oxidase and catalase, uniformly stirring to obtain a gel solution, and carrying out freeze drying on the gel solution to obtain the hydrogel. And heating, preserving heat and standing until the gel is formed. Oxidized sodium alginate (OSA) and gelatin (GEL) are coupled to generate pH-sensitive Schiff base bond crosslinking, glucose oxidase (GOx) and catalase (CAT) are loaded in the pH-sensitive Schiff base bond crosslinking, and dynamic gel capable of interacting with the environment is prepared; the hydrogel can interact with the micro-environment (pH, glucose and the like) of the chronic wound surface of the diabetes mellitus, and can be used as a micro-environment adaptive and adjustable dressing for treating the wound surface of the diabetes mellitus.

The invention discloses a high-strength and high-water-retention soybean meal-based adhesive and a preparation method thereof.The high-strength and high-water-retention soybean meal-based adhesive is prepared from, by weight, soybean protein meal, glucose oxidase, calcium sulfate oligomer, ethylene glycol diglycidyl ether and a dispersion medium, and the preparation method comprises the following steps that all the components are weighed according to the mass ratio; the preparation method comprises the following steps: dispersing glucose oxidase in a dispersion medium, uniformly dispersing soybean protein meal in the glucose oxidase dispersion liquid prepared in the step S1, heating for reaction, uniformly dispersing a calcium sulfate oligomer in the soybean protein meal dispersion liquid oxidized and catalyzed by the glucose oxidase prepared in the step S2, and uniformly dispersing the calcium sulfate oligomer in the soybean protein meal dispersion liquid oxidized and catalyzed by the glucose oxidase. And uniformly dispersing ethylene glycol diglycidyl ether in the dispersion liquid obtained in the step S3, and then stirring for 5 minutes. The adhesive disclosed by the invention has excellent properties in the aspects of bonding strength, water retention capacity, mildew resistance and sustainability.

The invention discloses a preparation method and application of an iron-based covalent organic framework enzyme-loaded biological cascade catalytic material, and belongs to the field of materials. An iron-based covalent organic framework material is prepared by adopting a one-pot method, and glucose oxidase is further loaded, so that the biological cascade catalytic material for glucose detection is prepared. The biological cascade catalytic material obtained by the preparation method disclosed by the invention combines the advantages of nano enzyme and natural enzyme, and has excellent catalytic efficiency and long catalytic life; the Fe-COFs prepared by the method disclosed by the invention is strong in peroxidase-like activity and high in enzyme loading capacity, can adapt to a cascade system loaded by various enzymes, and widens the application prospect of constructing a biological cascade catalytic system based on COFs loaded natural enzymes.

The invention discloses a method for reducing the total sugar content of fruit juice and sugar-reduced fruit juice. According to the method for reducing the total sugar content of the fruit juice provided by the invention, the fresh fruit juice is subjected to enzymolysis by adopting the compound polysaccharide enzyme, then the glucose oxidase and the catalase are added at the same time for enzymolysis, and the sugar-reduced fruit juice is obtained after enzyme deactivation, so that the total sugar content in the fruit juice can be remarkably reduced, and the total sugar content can be reduced by more than 50%. The composite polysaccharide enzyme, the glucose oxidase and the catalase in a specific ratio are combined for enzymolysis, so that the contents of cane sugar, glucose and fructose in the fruit juice can be simultaneously and efficiently reduced, a synergistic enzymolysis effect is achieved, and a better sugar reducing effect is achieved; when the sugar-reduced mango juice is prepared from the sugar-reduced mango juice, the content of sucrose in the mango juice can be reduced by 78.9%, the content of glucose can be reduced by 51.8%, and the content of fructose can be reduced by 6.53%. By adopting the method disclosed by the invention, the sugar reduction rate of the fruit juice is remarkably improved, and the prepared sugar-reduced fruit juice is good in flavor, low in sugar content, healthy and free of burden.

The invention discloses a microneedle system for continuous blood glucose monitoring, which comprises a microneedle array, an electrode system, a circuit connection and signal acquisition unit and a fixing and packaging structure, the working electrode is used for penetrating into the skin and making contact with intertissue fluid to control the diffusion speed of glucose and prevent glucose oxidase from losing, the surface of the working electrode is wrapped with glucose oxidase cross-linked and fixed through glutaraldehyde, one end of the electrode system is inserted into the microneedle, and the other end of the electrode system is connected with the circuit connection and signal acquisition unit. The fixing and packaging structure is arranged on the outer side of the circuit connecting and signal collecting unit and used for wrapping the electronic device and fixing the microneedle system to the skin surface. According to the microneedle system for continuous blood glucose monitoring, rapid and accurate detection of in-vivo blood glucose level changes is achieved, and minimally invasive property and good patient compliance can be achieved while the detection sensitivity and accuracy are guaranteed.