Immobilized enzyme biological catalyst, preparation method and application

A biocatalyst and immobilized enzyme technology, applied in biochemical equipment and methods, immobilized enzymes, enzymes, etc., can solve the problems of easy detachment of enzymes, achieve the effects of improving catalytic activity, reducing the amount of enzymes used, and reducing the cost of use

Inactive Publication Date: 2007-10-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of the study showed that the activity of the immobilized enzyme prepared by the physical adsorption method was 5 times that of the immobilized enzyme prepared by the covalent cross-linking method, but the enzyme adsorbed on the carrier was easy to fall off during use

Method used

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  • Immobilized enzyme biological catalyst, preparation method and application
  • Immobilized enzyme biological catalyst, preparation method and application
  • Immobilized enzyme biological catalyst, preparation method and application

Examples

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preparation example Construction

[0028] The preparation method of the present invention is described in detail below:

[0029] 1, physical adsorption method prepares catalyst of the present invention:

[0030] A certain weight of acicular CaCO 3 Add the particles into 50-200mL of deionized water, disperse them with an ultrasonic device, then add 10-50mL of ethanol and 0.01-3g of surfactant, and stir thoroughly for 15-30 minutes; then slowly add a certain amount of ethyl orthosilicate dropwise into the mixed solution, aged for 2-3 hours, filtered, washed several times with deionized water and ethanol, dried, calcined, and dissolved in hydrochloric acid to remove the template CaCO 3 , and finally made tubular hollow SiO 2 Mesoporous material carrier, spare; based on the weight percentage of the immobilized enzyme biocatalyst, add 80.00% to 99.99% of the carrier material of tubular hollow structure silica and 0.01% to 10.00% of at least one crystallizing enzyme or solution enzyme Into the deionized water, ace...

Embodiment 1

[0040] This example is to prepare the needle-shaped calcium carbonate template of the carrier: weigh a certain amount of industrial-grade quicklime, put it into hot water 7 to 8 times the quality of quicklime, and the temperature is about 70°C, digest it for a period of time, and separate it after cooling. Filter once with 80 mesh and 250 mesh standard sieves to obtain refined Ca(OH) 2 suspension, and then titrate the Ca(OH) with the calibrated EDTA standard solution 2 concentration, and then use the prepared Ca(OH) 2 The suspension was subjected to carbonization reaction, in this experiment, the gas phase (CO 2 Gas) is continuously fed in, the liquid phase circulates, contacts and reacts with the gas phase in the rotating packed bed. The specific steps of the carbonization reaction are as follows: (1) temperature adjustment: the rotating packed bed and the stirred tank are equipped with jackets, and the temperature of the reaction system is controlled by controlling the tem...

Embodiment 2

[0042] The acicular calcium carbonate that mainly contains striatum phase crystal form prepared by embodiment 1 is mixed with the suspension that concentration is 0.8mol / L, gets 200mL nano-calcium carbonate suspension and is placed in reactor, and starts to heat up and stir, stirring speed Control at 400-500r / min; prepare 500mL of sodium silicate solution with a concentration of 0.68mol / L and 10wt% dilute hydrochloric acid solution. When the temperature rises to 80°C, start to add sodium silicate solution dropwise, and add dilute hydrochloric acid at the same time, adjust the pH of the system between 8.5 and 9.5, and generate CaCO 3 / SiO 2 Core-shell structure materials. After all the sodium silicate solution is added dropwise into the system, stop adding acid, and stir and age at this reaction temperature, and the aging time is controlled at 4h, so that the SiO 2 in CaCO 3 Surface deposition, curing. The aged slurry was filtered, washed, dried at 105°C for 12h, and then c...

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Abstract

The invention relates to an immobilized enzyme biocatalyst and the manufacturing method and application. The immobilized enzyme biocatalyst regards tubular hollow silica dioxide dielectric hole material as a carrier external surface, inner surface and micropore in the wall of which are fixed biological enzyme molecule. The method includes physical adsorption cast investment or chemical coupling/ crosslinking method; the biocatalyst is provided with good enzyme dispersibility and high carrying quantity, high recovery ratio of enzymatic activity, low enzyme flow rate. The invention can fix penicillin acylating enzyme, glucose oxidase, peroxidase, cytase and so on, which also can be used for removing sugar in protein, removing sugar in total egg, removing oxygen in food , microbiological sensing device, antibiosis and disinfection reaction, wherein immobilized penicillin acylating enzyme biocatalyst can be used to hydrolyze penicillin potassium and manufacture 6-APA.

Description

technical field [0001] The invention relates to an immobilized enzyme biocatalyst, in particular to an immobilized enzyme biocatalyst with silicon dioxide as a carrier, a preparation method thereof, and an application in catalyzing the hydrolysis reaction of penicillin potassium salt. Background technique [0002] Enzyme-catalyzed processes have been successfully applied in the production of food, pharmaceuticals, agricultural chemicals, and increasingly in the field of organic chemical synthesis. However, in the practical application of the enzyme catalysis method, there are problems such as the unstable structure of the enzyme after it is separated from its natural environment, and most of the enzymes are water-soluble, and the enzyme will pollute the product after being dissolved in water, and it is difficult to recycle. Enzymatic Immobilization is the most successful method to overcome the above problems. For example: immobilized penicillin acylase (Immobilized Penicill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P37/00C12N9/00C12N11/00
CPCY02P20/50
Inventor 陈建峰肖清贵陶霞
Owner BEIJING UNIV OF CHEM TECH
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