Glucose detection sensor, and preparation and application methods thereof

Abstract

The invention discloses a glucose detection sensor, and preparation and application methods thereof. The sensor comprises a three-electrode system, wherein a graphene / chitosan / glucose oxidase deposition modified glassy carbon electrode is used as a working electrode, a platinum sheet is used as a counter electrode, and a calomel electrode is used as a reference electrode. In the detection process, by using the graphene / chitosan / glucose oxidase deposition modified glassy carbon electrode as the working electrode, the platinum sheet as the counter electrode and the calomel electrode as the reference electrode, an electrochemical work station is utilized to determine the glucose content. Compared with the traditional method, the invention has the advantages of higher sensitivity and higher specificity, and the low detection limit is 4.0*10<-7> mol / L; and meanwhile, the glucose sensor has the advantages of simple preparation process, stable performance and short sample detection time, can be recycled, and is convenient to operate.

Description

Technical field: [0001] The invention relates to a simple, rapid and specific glucose detection sensor and its preparation and use methods. Background technique: [0002] Diabetes is a worldwide epidemic disease, and the prevalence of middle-aged people over the age of 40 is particularly high. In Japan, diabetic patients account for nearly 10% of the population over the age of 40. The cause of diabetes is that the body cannot produce enough insulin or the cells do not respond to insulin, which causes the body to produce high blood sugar. There are many complications, including cardiovascular disease, kidney failure, blindness, etc. Therefore, it is of great significance to realize the simple, rapid and specific detection of glucose concentration in human blood for the treatment and control of diabetes. Among them, the high-sensitivity glucose detection materials and methods are an important research part of blood glucose detection. At present, the methods used to detect gl...

AI Technical Summary

Problems solved by technology

At present, the methods used to detect glucose are mainly enzymatic colorimetry and electrochemical methods. Recently, the electrochemical analysis method of direct electron transfer between glucose oxidase and the electrode surface has attracted more and more attention. However, there are two problems in this method. Problems to be solved: First, due to the existence of the protein insulating shell, the electron transfer of the enzyme becomes difficult; second, the enzyme directly adsorbed on the surface of the electrode is easily denatured and inactivated, and the above are not conducive to the realization of simple, rapid and specific glucose detection. detection

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