23results about How to "Wide linear range" patented technology

The invention belongs to the technical field of biology, and particularly relates to a method for determining the activity of an RNA enzyme inhibitor. According to the method, a DNA-RNA hybrid chain is used as a substrate for determining the activity of the RNA enzyme inhibitor (preferably the enzyme activity of the RNA enzyme inhibitor), and the cost of the method is far lower than that of a substrate cCMP of a traditional activity (enzyme activity) unit detection method (cyclic monophosphate (CMP) detection method).

The invention relates to a method for determining the total amount of impurity elements in a natural high-purity quartz mineral, which comprises the following steps of: preparing a laser sheet of the quartz mineral, observing and photographing by using a polarizing microscope, designing an analysis test point on a picture, and carrying out point-by-point analysis and determination on a laser ablation and inductively coupled plasma spectrum according to the design point. Accurate determination of impurity elements of quartz minerals in a natural high-purity quartz raw material is realized, comprehensive analysis and evaluation are carried out, and grade characteristics of the quartz minerals in the high-purity quartz raw material are further determined. The method is suitable for analysis and determination of impurity elements in natural quartz minerals, has the advantages of low detection limit, high sensitivity, wide linear range and the like, can be applied to effective and rapid evaluation, quality monitoring and the like of high-purity quartz raw materials, and provides quartz mineral raw materials with stable grades for the high-purity quartz sand industry.

The invention relates to a qualitative and quantitative method for liensinine perchlorate. The qualitative method comprises the following steps: weighing 1mg of liensinine perchlorate and 1mg of liensinine, uniformly grinding and tabletting the liensinine perchlorate and the liensinine with 100mg of potassium bromide respectively, acquiring an infrared spectrum of 4000-400cm <-1 > by adopting an infrared spectrometer, and comparing spectrograms to find that the peak shapes of the liensinine perchlorate and the liensinine are similar, the peak shapes of the liensinine perchlorate and the liensinine perchlorate are common characteristic peaks and the peak shapes of the liensinine perchlorate are unique characteristic peaks of the liensinine perchlorate and the liensinine are common characteristic peaks and the peak shapes of the The quantitative method comprises the following steps: weighing a series of liensinine perchlorate reference substances with different masses, uniformly grinding and tabletting the liensinine perchlorate reference substances and 100mg of potassium bromide respectively, collecting an infrared spectrum, carrying out integration on a 625cm <-1 > characteristic peak to obtain a peak area, and drawing a standard curve by taking the peak area as a vertical coordinate and taking the sampling mass of the reference substances as a horizontal coordinate; the method comprises the following steps: weighing a sample to be detected, collecting an infrared spectrum, integrating a 625cm <-1 > characteristic peak to obtain a peak area, substituting the peak area into the standard curve equation, calculating the mass of the liensinine perchlorate in the sample, and substituting the mass into the content calculation formula to obtain the content of the liensinine perchlorate in the sample. The method is good in precision, repeatability and stability, wide in linear range, high in linear correlation coefficient and high in recovery rate, the problem that liensinine interference cannot be avoided in an existing content determination method is effectively solved, whether liensinine perchlorate is generated or not can be qualitatively judged, and the quantity of liensinine perchlorate generated can be quantitatively detected; and a feasible new method is provided for production process optimization and quality evaluation of liensinine perchlorate.

This invention discloses a method for quantitatively detecting muscle inositol in blood. It employs an electrochemical method and specifically includes the following steps: a) preparation of a series of standard working solutions; b) preparation of a blank sample solution; c) plotting a standard curve using differential pulse voltammetry (DPV) with the blank sample solution and the series of standard working solutions; d) preparation of the test sample solution; e) determination of the muscle inositol content in the test sample. Experiments have demonstrated that the method of this invention yields accurate and reliable results and can be used for clinical blood testing.

The invention discloses a preparation method and application of a carboxyl functionalized MON (at) ZIF-8-NH2 solid phase microextraction material. The preparation method comprises the following steps: taking ZIF-8-NH2 as an inner core and MON-2COOH as a shell to obtain a carboxyl-functionalized MON-coated ZIF-8-NH2 material, then fixing the carboxyl-functionalized MON-coated ZIF-8-NH2 material on a substrate by adopting a physical coating technology to obtain a solid-phase micro-extraction coating fiber based on carboxyl-functionalized MON-coated ZIF-8-NH2, and using the extraction fiber to measure hydroxyl polycyclic aromatic hydrocarbon in urine. The solid-phase micro-extraction coating fiber disclosed by the invention is thinner in coating, better in extraction effect, longer in service life and higher in stability. The method can be used for extracting various hydroxyl polycyclic aromatic hydrocarbons in urine at the same time by being combined with liquid chromatography-mass spectrometry, and has good stability, meanwhile, the determination method has excellent accuracy, and the recovery rate and the detection limit meet the detection requirements of trace analysis.

The invention belongs to the technical field of magnetic solid-phase extraction and analysis. The invention provides a method for quantitatively detecting bisphenol pollutants in traditional Chinese medicines for soil and roots based on a magnetic MOF (at) COF porous composite material combined with LC-MSMS. The method comprises the steps of preparation of the magnetic MOF (at) COF porous composite material, sample pretreatment and LC-MSMS detection and quantitative analysis. The magnetic MOF (COF) porous composite material disclosed by the invention has rich hierarchical pore structures and high specific surface area, not only retains regular pore channels and active sites of a metal organic framework (MOF), but also is connected with a covalent organic framework (COF), so that the stability of the material is greatly improved. Meanwhile, the material is mild in preparation condition, low in cost and excellent in adsorption performance. Compared with the existing method, the detection method provided by the invention has the advantages of more thorough extraction, lower detection limit, higher recovery rate, simpler operation and lower cost, and is suitable for two matrixes of soil and root system traditional Chinese medicines.

The application discloses an antibody against ochratoxin A and application thereof. The application uses an artificial antigen obtained by coupling ochratoxin A and a carrier protein as an immunogen to prepare a monoclonal antibody against ochratoxin A. The monoclonal antibody can sensitively and specifically detect ochratoxin A, and the minimum detection limit (LOD) is 0.18 ng / mL. In addition, the half inhibition (IC 50 ) of the icELISA for ochratoxin A established by using the monoclonal antibody against ochratoxin A is 1.42 ng / mL, and the linear range is 0.34-5.40 ng / mL. That is, the application provides an antibody capable of detecting ochratoxin A and a method for detecting ochratoxin A, and has the advantages of simplicity, rapidness, strong specificity, wide linear range and high sensitivity.

A lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe and a detection method of sugar components contained therein, comprising: water decoction extraction and concentration of traditional Chinese medicine decoction pieces constituting the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe to obtain water extract dry products, preparation of standard solution from the water extract dry products, liquid chromatography-electrospray detection by high performance liquid chromatography-electrospray detector to obtain standard liquid chromatogram and mark the characteristic peaks therein, then comparison with the liquid chromatogram of the detected decoction / patent medicine obtained by the same method to determine whether the detected decoction / patent medicine belongs to the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe, and to determine the six sugar substances contained in the detected decoction / patent medicine, and further comparison with the area of the corresponding sugar characteristic peak in the standard liquid chromatogram to determine the content of the corresponding sugar. The detection method and the standard provided by the application can be used as the basis for formulating the quality control standard and the quality detection standard of the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe decoction / patent medicine.

The invention provides an electrochemical sensor constructed based on an NP-G-AuPd NFs composite material. The electrochemical sensor comprises a working electrode and a sensing material modified on the working electrode, the sensing material is a nitrogen and phosphorus co-doped graphene-AuPd nanoflower composite material. Therefore, when the AuPd NFs and the NP-G are mixed, the AuPd NFs and the NP-G can be embedded in the middle of the graphene, the graphene is stripped to present a single-layer yarn-shaped structure, the conductivity of the graphene can be enhanced, the larger specific surface area of the graphene is a good interface for loading the AuPd NFs, and the NP-G and the nano material are mixed to finally prepare the NP-G-AuPd NFs composite material with better conductivity. An electrochemical sensor based on the NP-G-AuPd NFs composite material is constructed to detect diclofenac sodium, and a DPV test result shows that the composite material has good electro-catalytic performance and can be used for determining an actual sample.

This invention belongs to the field of materials synthesis and analysis, specifically relating to a heterojunction composite material Co. 1‑x Preparation method of S / MoS2 and its application in sulfide detection. This invention is the first to apply three-dimensional Co... 1‑x Two-dimensional MoS2 nanosheets were grown in situ on S nanocubes to prepare a 3D / 2D heterostructure composite material Co. 1‑x S / MoS2. Compared to the original C o1‑x Compared to S and MoS2, the obtained Co has a 3D / 2D heterostructure 1‑x The peroxidase catalytic activity of S / MoS2 is significantly enhanced. In the presence of sulfide ions, Co... 1‑x The peroxidase catalytic activity of S / MoS2 can be selectively inhibited, thereby enabling the development of a Co-based... 1‑x A colorimetric detection method catalyzed by S / MoS2 nanozymes is used for the detection of sulfur ions in environmental water samples, and it has high specificity.

The invention relates to a qualitative and quantitative method for isoliensinine perchlorate. The qualitative method comprises the following steps: weighing 1mg of isoliensinine perchlorate and 1mg of isoliensinine, uniformly grinding and tabletting the isoliensinine perchlorate and the isoliensinine with 100mg of potassium bromide respectively, acquiring an infrared spectrum of 4000-400cm <-1 > by adopting an infrared spectrometer, and comparing the spectrograms to find that the peak shapes of the isoliensinine perchlorate and the isoliensinine are similar, the peak shapes of the isoliensinine perchlorate and the isoliensinine perchlorate are common characteristic peaks and the peak shapes of the isoliensinine perchlorate are unique characteristic peaks of 625cm <-1 >. The quantitative method comprises the following steps: weighing a series of isoliensinine perchlorate reference substances with different masses, uniformly grinding and tabletting the isoliensinine perchlorate reference substances and 100mg of potassium bromide respectively, collecting an infrared spectrum, carrying out integration on a 625cm <-1 > characteristic peak to obtain a peak area, and drawing a standard curve by taking the peak area as a vertical coordinate and taking the sampling mass of the reference substances as a horizontal coordinate; the method comprises the following steps: weighing a sample to be detected, collecting an infrared spectrum, integrating a 625cm <-1 > characteristic peak to obtain a peak area, substituting the peak area into the standard curve equation, calculating the mass of the isoliensinine perchlorate in the sample, and substituting the mass of the isoliensinine perchlorate in the sample into the content calculation formula to obtain the content of the isoliensinine perchlorate in the sample. The method is good in precision, repeatability and stability, wide in linear range, high in linear correlation coefficient and high in recovery rate, the problem that an existing content determination method cannot avoid interference of isoliensinine is effectively solved, whether isoliensinine perchlorate is generated or not can be qualitatively judged, and the amount of isoliensinine perchlorate generated can be quantitatively detected. And a feasible new method is provided for production process optimization and quality evaluation of the isoliensinine perchlorate.

The invention discloses a fenaminosulf hapten, an artificial antigen, an antibody and a preparation method and application thereof. According to the fenaminosulf hapten disclosed by the invention, a carboxyl active group with a spacer arm with a certain length is introduced to a sulfocarboxyl group of fenaminosulf, so that the fenaminosulf hapten can be coupled with a carrier protein to obtain an artificial antigen for immunization; the fenaminosulf hapten retains all characteristic groups of fenaminosulf, the original structural characteristics of the fenaminosulf are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. The fenaminosulf hapten is used for preparing an artificial antigen, and a monoclonal antibody with high detection sensitivity and strong specificity is prepared from the fenaminosulf hapten. According to indirect competitive ELISA based on the fenaminosulf monoclonal antibody, the lowest detection limit of fenaminosulf is 2.45 ng / mL, IC50 is 14.80 ng / mL, the linear range is 4.75-46.06 ng / mL, the detection sensitivity is high, and the linear range is wide.

The present application relates to a kind of preparation method of redox probe based on metal organic framework derived nanosheet and its product and application, belong to the technical field of preparation of redox probe.The present application is mainly to form super-thin metal organic framework material by self-assembly of metal ion (Cu 2+ Or Co 2+ ) and organic ligand, continue to react with ascorbic acid (AA), chloroauric acid (HAuCl4) to obtain metal-UMOFNs@Au NPs, then mixed with alpha-fetoprotein antibody (anti-AFP), carcinoembryonic antibody (anti-CEA) and glucose oxidase (GOD) after incubation to obtain redox probe (metal-UMOFNs@Au NP-Ab2-GOD).In addition, the redox probe prepared by the present application can be used to prepare sandwich-type electrochemical immunosensor capable of simultaneously detecting CEA and / or AFP, and the immunosensor has higher sensitivity, good selectivity and stability.

The invention belongs to the technical field of analytical chemistry, and particularly relates to an aptamer functionalized COF material and application thereof in aflatoxin detection. Specifically, vinyl COF (Tpb-Dva-COF) is used as a substrate, amino groups are introduced through post-modification, and aflatoxin nucleic acid aptamers are covalently fixed on the surface of the substrate by using a chemical crosslinking method. The invention also establishes a method for detecting aflatoxins B1, B2, G1 and G2 in food and traditional Chinese medicinal materials by combining ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) based on the material as a solid phase extraction (SPE) adsorbent, and provides an efficient and reliable solution for accurate detection of trace aflatoxins in a complex matrix, so that the material has good practical application value.

This invention discloses a method for detecting budesonide content in budesonide nasal spray, relating to the field of chemical analysis technology. The steps are as follows: First, a budesonide nasal spray sample is taken, cellulase is added for enzymatic hydrolysis, then diluted with the mobile phase, shaken well, centrifuged, and filtered to obtain a test solution; then, a budesonide reference standard is taken, dissolved and diluted with the mobile phase to obtain a reference solution; the test solution and the reference solution are injected into a high-performance liquid chromatograph for detection, respectively, to obtain peaks of budesonide isomer A and budesonide isomer B; finally, the content is calculated by the sum of the peak areas of budesonide isomer A and budesonide isomer B using the external standard method. This method, by introducing a cellulase enzymatic hydrolysis step in the sample pretreatment, effectively hydrolyzes the co-treated microcrystalline cellulose and sodium carboxymethyl cellulose, achieving complete separation of the active ingredient budesonide from the excipients.

The present application relates to the technical field of molecular detection and diagnosis, and particularly relates to a tetrahedral probe, a preparation method thereof and application of the tetrahedral probe in detection of apurinic / apyrimidinic endonuclease 1 (APE1). The DNA tetrahedral probe is formed by self-assembly of a frame DNA chain, a triple helix complex and a hairpin H chain. The frame DNA chains interact to form a stable tetrahedral skeleton; when APE1 exists in a sample, it can specifically recognize and cut apurinic / apyrimidinic sites on the triple helix complex and the hairpin H chain, trigger a cascade reaction and realize signal amplification; nucleic acid drugs in the probe are released with the cutting, and thus the synergy of detection and treatment is realized. The technical solution can solve the technical problem of unsatisfactory sensitivity and accuracy of in vitro detection of APE1 enzyme in the prior art, has low detection limit and wide linear range. The technical solution has important clinical application value and scientific research significance, and is expected to promote the development of non-small cell lung cancer diagnosis and treatment technology.

The invention relates to a qualitative and quantitative method of lotus plumule total alkali perchlorate. The qualitative method comprises the following steps: weighing 1mg of lotus plumule total alkali perchlorate and 1mg of lotus plumule total alkali, uniformly grinding and tabletting the weighed lotus plumule total alkali perchlorate and the weighed lotus plumule total alkali perchlorate and 100mg of potassium bromide respectively, collecting 4000-400cm <-1 > infrared spectrums by adopting an infrared spectrometer, and comparing spectrograms to find that the two peaks are similar in shape, 1515cm <-1 > and 110cm <-1 > are common characteristic peaks, but 625cm <-1 > is a unique characteristic peak of the lotus plumule total alkali perchlorate. The quantitative method comprises the following steps: weighing a series of lotus plumule total alkali perchlorate reference substances with different masses, uniformly grinding and tabletting the reference substances and 100mg of potassium bromide respectively, collecting an infrared spectrum, carrying out integration on a 625cm <-1 > characteristic peak to obtain a peak area, and drawing a standard curve by taking the peak area as a vertical coordinate and taking the sampling mass of the reference substances as a horizontal coordinate; weighing a to-be-detected sample, collecting an infrared spectrum, integrating a 625cm <-1 > characteristic peak to obtain a peak area, substituting the peak area into the standard curve equation, calculating the mass of the lotus plumule total alkali perchlorate in the sample, and substituting the mass of the lotus plumule total alkali perchlorate in the sample into the content calculation formula to obtain the content of the lotus plumule total alkali perchlorate in the sample. The method is good in precision, repeatability and stability, wide in linear range, high in linear correlation coefficient and high in recovery rate, effectively solves the problem that an existing content determination method cannot avoid interference of lotus plumule total alkali, can qualitatively judge whether lotus plumule total alkali perchlorate is generated or not, can quantitatively detect how many lotus plumule total alkali perchlorate is generated, and can be used for determining the content of the lotus plumule total alkali perchlorate. And a feasible new method is provided for production process optimization and quality evaluation of the lotus plumule total alkali perchlorate.

The application belongs to the field of biological medicine detection, and particularly relates to a monitoring method for purification efficiency of acridinium ester labeled antibody, comprising the following steps: (1) sample separation: separating free acridinium ester from acridinium ester labeled antibody in a to-be-detected sample by using ultrafiltration centrifugation or high performance liquid chromatography; (2) establishing a standard curve; (3) chemiluminescence detection and quantification; and (4) efficiency calculation. The monitoring method for purification efficiency of acridinium ester labeled antibody and the application thereof can distinguish and separately quantify the monitoring method for free acridinium ester and bound acridinium ester, and evaluate the desalting efficiency and apparent binding rate of a purification process.

The invention discloses sgRNA, an editing plasmid, a recombinant plasmid, an AviTag engineered bacteriophage, a magnetic bacteriophage probe, and a construction method and application thereof. The invention further discloses an enrichment separation and quantitative detection method for the pseudomonas aeruginosa. The magnetic bacteriophage probe can rapidly and specifically enrich pseudomonas aeruginosa in complex matrixes such as serum, infectious microbes and interference components are removed through magnetic separation, and sensitive quantitative detection of target bacteria is achieved in combination with an ATP bioluminescence system. According to the invention, by optimizing the AviTag-linker configuration, the display and biotinylation efficiency of the AviTag tag on the surface of the bacteriophage is remarkably improved, on the premise of keeping the titer of the bacteriophage and the stability of a host spectrum, the capture capability of the magnetic bacteriophage probe is enhanced, and low detection limit, short detection time and good repeatability are realized.

This invention constructs a sandwich-structured sensor based on the synergistic effect of magnetic molecular imprinting and boric acid affinity for rapid visual detection of Klebsiella pneumoniae. The sensor uses Fe3O4@SiO2 with surface-grafted double bonds as a carrier and Klebsiella pneumoniae as a template to prepare magnetic molecularly imprinted polymers (MIPs) with specific recognition cavities via free radical polymerization. Simultaneously, a ZIF-8 material loaded with curcumin was synthesized and modified with p-carboxyphenylboronic acid to construct a ZIF-8@Cur@PBA signal probe. The boric acid groups specifically bind to the cis-diol groups of bacterial surface glycoproteins. In the presence of the target bacteria, the MIPs and probe form a sandwich structure mediated by the bacteria. After magnetic separation, hydrochloric acid is added to cleave ZIF-8, releasing curcumin and amplifying the signal. The signal is then neutralized with sodium hydroxide to restore color, enabling visual semi-quantitative detection through visual observation, while quantitative analysis is performed by measuring ultraviolet absorbance. This sensor exhibits excellent selectivity, anti-interference ability, and stability, providing a new method for rapid and sensitive detection of Klebsiella pneumoniae, and has important practical application value.

The invention belongs to the technical field of material analysis, and provides a method for rapidly detecting the migration quantity of chloropropanol compounds in cigarette paper, which comprises the following steps: carrying out a water phase migration experiment on a cigarette paper sample to be detected to obtain a migration liquid; adding deuterated chloropropanol into the migration liquid, mixing with an internal standard stock solution, and carrying out salting-out auxiliary liquid-liquid extraction, dehydration purification and nitrogen blowing concentration to obtain a liquid to be detected; a gas chromatography-tandem mass spectrometry method is adopted to measure the to-be-measured liquid, a chromatographic column is an ultra-high inert bonded polyethylene glycol capillary chromatographic column, and data are collected in a multi-reaction monitoring mode; the migration amount of the chloropropanol compounds in the sample is calculated through an internal standard method, and the chloropropanol compounds comprise 1, 3-dichloro-2-propanol and 3-chloro-1, 2-propylene glycol. The method does not need a derivatization step, is high in detection accuracy, and can realize rapid detection and batch analysis.

The application discloses a conjugated microporous polymer material for detecting heavy metal iron in drinking water and a synthesis method and application thereof. The conjugated microporous polymer material for detecting heavy metal iron in drinking water is synthesized by a solvothermal reaction method of terephthalic dihydrazide and 2,5-dimethoxy terephthaldehyde. The mass ratio of terephthalic dihydrazide to 2,5-dimethoxy terephthaldehyde is 2:5 to 5:2 during the synthesis.