118results about How to "Wide linear range" patented technology

The invention discloses a construction method and application of an electrochemical luminescence (ECL) antifouling sensor based on zwitter-ion hydrogel, and belongs to the technical fields of photoelectric sensing analysis, interface antifouling and environmental science. According to the invention, the hydrogel based on sulfobetaine methacrylate zwitterions and bovine serum albumin is used as the antifouling coating, so that the adsorption of biological proteins and bacteria is effectively hindered, and the service life of the sensor is prolonged. Meanwhile, the interior label Au (at) luminol is packaged in the antifouling hydrogel, so that the integration of interface antifouling and ratio detection is realized, the detection accuracy is improved, and the construction process of the ECL sensor is simplified. The constructed ECL anti-pollution sensor has high sensitivity to chloramphenicol detection, the detection limit is 0.39 pM, and the ECL anti-pollution sensor is suitable for trace detection of antibiotics in seawater.

According to the method, phosphomolybdic acid, copper chloride and ferric chloride serve as precursors, copper and iron-containing phosphomolybdic acid nano-enzyme (Cu, Fe-PMs) with peroxidase-like activity is synthesized through a microwave method, under the acidic condition, on one hand, the surface of Cu and Fe-PMs carries negative charges, high affinity is generated between Cu and Fe-PMs and a substrate TMB with positive charges, and on the other hand, the surface of Cu and Fe-PMs has negative charges; on the other hand, Cu / Fe / Mo three-metal redox couple accelerates the regeneration of Fe < 2 + > and plays an important role in the activity of peroxidase, and meanwhile, due to the introduction of sodium dehydroacetate, Cu and Fe in Cu and Fe-PMs and pyranoid ring and keto O in sodium dehydroacetate interact through coordinate bonds to cause electron interaction, so that the peroxidase-like activity of Cu and Fe-PMs is enhanced, and the peroxidase-like activity of Cu and Fe-PMs is improved. And the Fenton reaction is enhanced through transfer of electrons among the sodium dehydroacetate, the Cu and the Fe-PMs. When Cu and Fe-PMs oxidize TMB and H2O2, sodium dehydroacetate enhances the absorbance of the system, and on the basis, a novel colorimetric rapid detection method of sodium dehydroacetate is established.

The invention discloses an electrochemical detection method of dopamine, and belongs to the technical field of analysis. According to the method, SnS2 quantum dots are loaded on Zn-MOF and modified on an electrode, then gold nanoparticles and a sulfydryl-containing dopamine aptamer single chain are sequentially fixed on the electrode, an Apt / AuNPs / SnS2QDs (at) Zn-MOF / GCE composite electrode (in an open state) is formed, an electrochemical signal of the composite electrode is greatly improved, and based on specific recognition between dopamine and an aptamer chain, the electrochemical performance of the composite electrode is improved. A large cavity of Zn-MOF is used for packaging SnS2 quantum dots with excellent electrochemical properties, electrochemical signal amplification is achieved, when a target object dopamine exists, dopamine can quench an ECL signal, an electric signal is obviously reduced (in a closed state), and therefore a linear relation between the change of the electrochemical signal and the concentration of dopamine is constructed, and the electrochemical property of the electrochemical signal is improved. Therefore, high-sensitivity and high-selectivity detection of dopamine is realized.

The invention discloses a method for measuring the content of rare earth elements in a single rare earth compound by turbidimetry. Comprising the following steps: 1, preparing a fluoride solution and a 50% nitric acid aqueous solution; 2, preparing a rare earth element standard solution; step 3, testing the rare earth compound sample by using turbidimetry; and 4, calculating a detection result: calculating the content of the rare earth elements in the sample according to a formula. The method can be used for testing the content of single rare earth with different content levels in the sample. The method has the advantages of wide linear range, high precision, good accuracy, low cost, simplicity in operation, high detection efficiency and the like.

The invention provides a label-free magnetic self-assembly nanoprobe, a construction method thereof and application of the label-free magnetic self-assembly nanoprobe in detection of PrPC protein. The label-free magnetic self-assembly nanoprobe disclosed by the invention comprises a magnetic Fe3O4 / Fe2O3 (at) Au heteroplasmon nanorod array and a sulfhydrylation aptamer nucleic acid chain which is combined with the magnetic Fe3O4 / Fe2O3 (at) Au heteroplasmon nanorod array through an Au-S bond; a biosensor prepared from the label-free magnetic self-assembly nanoprobe is good in reproducibility and stability; the RSD value of multiple times of detection (intraday / day) is 1.55%; the storage time of multiple groups of sensors is 0-15 days, and the change of response signal values does not exceed 4%; the biosensor has a relatively low detection limit (1pg / mL), a relatively wide linear range (1 * 10 < pg / mL >-1 * 10 < 5 > pg / mL) and relatively good sensitivity, has very good application in detection of PrPC, and has very good practicability.

The invention discloses a colorimetric-fluorescent bimodal nanoprobe for detecting cMyBP-C and a micro-fluidic chip, and belongs to the technical field of biomedical detection and in-vitro diagnosis. The colorimetric-fluorescent bimodal nanoprobe which takes UCNPs as a core and MOF as a shell and is loaded with horse radish peroxidase and a labeled antibody is prepared by providing a layer-by-layer self-assembly technology; the micro-fluidic chip is prepared according to the probe, the micro-fluidic chip can realize cMyBP-C colorimetric and fluorescence signal bimodal detection, and the technical problems of limited sensitivity, susceptibility to background interference, limited linear range, photobleaching phenomenon, signal instability and the like in single signal detection are solved.

The invention provides a triglyceride determination kit, the kit comprises a reagent 1 and a reagent 2, the reagent 1 comprises the following main components: piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH 6.8), glycerophosphate oxidase (GPO), peroxidase (POD), triadenosine phosphate (ATP), chromogen, a surfactant, a stabilizer and a preservative; and the reagent 2 comprises the following main components: a piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH is 6.5), lipoprotein esterase (LPL), glycerol kinase (GK), 4-aminoantipyrine (4-AA), a surfactant, a stabilizer and a preservative. By optimizing a chromogen substrate in the reagent and adding potassium ferrocyanide, the linear range of the prepared kit is wider, and the bottle opening test is more stable.

The invention belongs to the technical field of colorimetric chemical sensors, and particularly relates to a preparation and application method of a smartphone-assisted surface molecular imprinting colorimetric sensor for detecting norfloxacin, magnetic Fe3O4 (at) KH540 nanoparticles are taken as a matrix, magnetic norfloxacin surface molecular imprinting nano-enzyme MMIPs is prepared by adopting a surface molecular imprinting technology, then the MMIPs, H2O2 and 3, 3 ', 5, 6, 7-tetramethyl-3, 5, 6-tetramethyl-3, 5, 6-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3 The colorimetric sensor for detecting norfloxacin is constructed by carrying out a chromogenic reaction on 3, 3 ', 5, 5'-tetramethyl benzidine TMB and cooperating with a smartphone. The sensor has specific recognition selectivity and binding affinity for NOR, is simple to operate, high in detection speed and low in cost, does not need expensive instruments, and can be suitable for field analysis.

The invention belongs to an advanced material technology, and relates to a novel rhodamine derivative, in particular to a 2-aminothiazolyl rhodamine 6G derivative as well as a preparation method and application thereof. Rhodamine 6G and 2-aminothiazole are used as raw materials, the 2-aminothiazolyl rhodamine 6G derivative RGAT is prepared through a one-step reaction, on one hand, RGAT shows excellent selectivity and very high sensitivity to Fe < 3 + >, fluorescence enhancement is remarkable, the linear range of Fe < 3 + > is wide, in the Fe < 3 + > concentration range of 0-50 M, a good linear relation with the Fe < 3 + > concentration is shown, and the detection limit is as low as 2.4 nM; on the other hand, the RGAT has better antibacterial performance on gram-positive bacteria and gram-negative bacteria than commercially available bleaching water, and the RGAT is very low in cytotoxicity, so that the RGAT has a good application prospect in the fields of environmental monitoring and biological medicine.

The invention discloses a noble metal core-shell-satellite assembly AuNR (at) Ag (at) SiO2-AuNP as well as a preparation method and application thereof in detection of vomitoxin, and belongs to the technical field of nano materials and detection of vomitoxin. According to the nanoparticles, AuNR (at) Ag (at) SiO2-AuNP nanoparticles are obtained by growing AuNP satellites on the surfaces of AuNR (at) Ag (at) SiO2 nanoparticles through a silicon layer surface reduction method. The preparation method has the beneficial effects that the prepared AuNR (at) Ag (at) SiO2-AuNP nanoparticles can be used for detecting vomitoxin, and the unique advantages of SERS and an immunochromatography ICA technology can be fully exerted by combining the SERS and the immunochromatography ICA technology, so that the AuNR (at) Ag (at) SiO2-AuNP nanoparticles have the advantages of high sensitivity, high specificity, convenience in operation, stable performance, low cost and the like in the aspect of detecting DON.

The invention provides a crystal phase impurity analysis method, and belongs to the technical field of analytical chemistry. The method comprises the following steps: acquiring an X-ray diffraction pattern of a sodium zirconium cyclosilicate sample to be detected; according to the standard curve or standard equation of the crystal phase impurities and the X-ray diffraction pattern of the sodium zirconium cyclosilicate sample to be detected, the content of the crystal phase impurities in the sodium zirconium cyclosilicate sample to be detected is obtained; the standard curve is obtained by fitting the ratio of the mass of the crystal phase impurities to the mass of the sodium zirconium cyclosilicate in a standard curve sample and the ratio of the diffraction intensity of characteristic diffraction peaks of the crystal phase impurities to the diffraction intensity of characteristic diffraction peaks of the sodium zirconium cyclosilicate in an X-ray diffraction pattern of the standard curve sample; the standard equation is a linear equation corresponding to the standard curve; the standard curve sample is a mixture of a blank sodium zirconium silicate sample and a crystal phase impurity reference substance. By adopting the method disclosed by the invention, the content of the crystal phase impurities such as CPA and CPB in the sodium zirconium cyclosilicate can be simply, conveniently, quickly and accurately detected.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The invention discloses an analysis and detection method and device based on a weighting function and multi-brightness fusion, and belongs to the field of immunochromatography analysis and detection. The analysis and detection method comprises the steps that a multi-brightness image set is obtained under multiple illumination intensities, in the process of restoring a brightness response function of an image acquisition system, an HDR algorithm based on multi-brightness fusion is used, weights of different brightness images are adjusted by adopting a segmented weight function, parameters of the segmented weight function are finely regulated and controlled, and the brightness response function of the image acquisition system is recovered. The characteristics of each concentration section in immunochromatography are reflected more finely, and the optimal weighting function is established to enhance the accuracy of brightness response function fitting, so that the immunochromatography HDR image with the highest quality is recovered, and the linear range of quantitative detection is expanded.

The present invention relates to the field of electrochemical detection technology, and relates to an Ag3PO4-UiO-66 / apt / GO / GCE electrochemiluminescence sensor and its application. The sensor comprises a glassy carbon electrode, the outer surface of which is modified in sequence by Ag3PO4-UiO-66 as a resonance energy donor, apt, a ligand adapted to an object to be detected, and GO as a resonance energy receptor, and Ag3PO4-UiO-66 and GO are connected by apt; Ag3PO4-UiO-66 is a UiO-66 material loaded by Ag3PO4. The sensor has the advantages of high detection sensitivity, fast detection speed, strong specificity, wide linear range, and easy use.

The present invention relates to the field of biotechnology. The present invention provides a method and a kit for simultaneously detecting 16 pathogenic microorganisms in raw milk using a liquid chip, and respectively designs specific upstream primers, specific downstream primers, probes and microspheres for different strain sequences, and then uses the multiple PCR products obtained after multiple PCR amplification to mix and hybridize with the liquid chip working solution with microsphere probes, and then incubate with SAPE, and then use the machine to determine the type of pathogenic microorganisms and their detection amount based on the detected microsphere fluorescence code and the MFI value of the fluorescence intensity of the reporter molecule on the microsphere. The detection method of the present invention requires a small amount of samples, and can achieve rapid, high-sensitivity, high-specificity, and high-throughput detection of multiple pathogenic microorganisms; it can provide a new method selection for laboratory testing of raw milk quality and safety, and better ensure the quality and safety of products and people's food safety.

The invention discloses preparation of an L / J-CDs-based ratiometric fluorescent probe and application of the ratiometric fluorescent probe to cetirizine hydrochloride detection, and belongs to the technical field of chemical synthesis and analysis. The ratiometric fluorescent probe is composed of a composite system of a carbon dot L-CDs solution emitting blue fluorescence and a carbon dot J-CDs solution emitting orange fluorescence, the blue fluorescence of the L-CDs in the composite system is quenched due to the addition of cetirizine hydrochloride molecules, the J-CDs are used as a reference signal, the fluorescence color is not changed, and the ratio of the fluorescence to the carbon dot L-CDs is determined. Cross reference correction is carried out through the intensity ratio of two emission peaks under the same excitation wavelength, the effects of reducing errors and improving accuracy are achieved, and visual qualitative detection assisted by naked eyes and smart phones based on an ultraviolet lamp can be achieved through the ratio fluorescent probe.

The invention relates to the technical field of biological detection, in particular to a kit and method for detecting NfL, the kit comprises a magnetic bead coated with a capture antibody, a non-functional auxiliary magnetic bead, an NfL detection antibody coupled with a first marker, beta-galactosidase coupled with a second marker, an enzyme reaction luminescent substrate and an NfL standard substance; the capture antibody in the magnetic bead coated with the capture antibody is used for being specifically combined with an NfL antigen; the first marker in the NfL detection antibody coupled with the first marker is connected with the second marker in the beta-galactosidase coupled with the second marker, and the NfL detection antibody is suitable for being specifically combined with an NfL antigen. The kit provided by the invention has the advantages of higher sensitivity, less background signal interference, wider linear range, convenience for full-automatic detection instruments and the like.

The invention belongs to the technical field of environment detection, and particularly relates to a method for analyzing total nitrogen in water. The method includes: preparing a nitrogen standard solution; injecting a nitrogen standard solution into the quartz tube by using an injector, and vaporizing the nitrogen standard solution in the quartz tube; introducing the vaporized nitrogen standard solution into a reaction chamber by using argon, carrying out combustion cracking reaction on the vaporized nitrogen standard solution in the reaction chamber to convert into NO, reacting NO with O3 gas to convert into excited state NO2 *, and emitting photons when the excited state NO2 * jumps to a ground state; and the photomultiplier is used for detecting the photon intensity according to a specific wavelength. The method is wide in linear range, the water sample does not need to be diluted during detection, and errors caused in the dilution process are avoided. The method is good in detection repeatability and high in accuracy, the analysis time is shortened by 50 min compared with an existing method, and the analysis time and the labor intensity are effectively reduced. The sample can be directly injected, the environmental pollution is avoided, and the pollution or element loss possibly caused in the pretreatment process of the sample is avoided.

The present invention provides an MMP7 detection kit and use thereof in preparing a product for diagnosing MMP7-related diseases. The kit comprises a magnetic microsphere solution M, a biotinylated antibody solution R1 and a luminescent marker R2, and exhibits the advantages of stable performance, high sensitivity, wide linear range, convenient operation, strong clinical practicality, etc. in the detection of MMP7.

The invention belongs to the technical field of biology, and particularly relates to a method for determining the activity of an RNA enzyme inhibitor. According to the method, a DNA-RNA hybrid chain is used as a substrate for determining the activity of the RNA enzyme inhibitor (preferably the enzyme activity of the RNA enzyme inhibitor), and the cost of the method is far lower than that of a substrate cCMP of a traditional activity (enzyme activity) unit detection method (cyclic monophosphate (CMP) detection method).

The present invention discloses a method for near-infrared driven plasma enhanced nanoenzyme colorimetric detection of histamine, wherein the method synthesizes graphite-like carbon nitride (g-C3N4) by pyrolysis, and then synthesizes composite nanomaterial Cu / MOF / C3N4 by Zn(NO3)2·6H2O, 2-hydroxyterephthalic acid and g-C3N4. Under near-infrared driven plasmon excitation, Cu / MOF / C3N4 exhibits significantly enhanced laccase-like activity, and the photothermal effect induced by localized surface plasmon resonance improves the laccase-like activity. When Cu / MOF / C3N4 oxidizes 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP), histamine can be adsorbed on the surface of Cu / MOF / C3N4, inhibiting the generation of semiquinone free radicals, thereby inhibiting the activity of Cu / MOF / C3N4-type laccase. The histamine concentration is in the linear range of 0.005~22.5 mg / L, causing the red color to become lighter. Therefore, a new highly sensitive colorimetric detection method for histamine was developed with a detection limit of 1 μg / L. The method of the present invention can only selectively detect histamine, and there is no interference from other substances.

The present invention discloses a label-free ratio fluorescence type antigen detection method. The present invention comprises the following steps: designing a single-stranded DNA, comprising a recognition DNA sequence for CRISPR, an aptamer for a target antigen, and the two are separated by several consecutive T bases to construct component A; using the target antigen as a target, modifying the antibody of the target antigen on a 96-well plate to construct component B; connecting the surface carboxylated silica microspheres to a DNA chain modified with an amino group at the end, and complementary pairing of the auxiliary DNA with the amino group DNA to form a double strand to construct component C; adding component A, component B, component C, copper ions and ascorbic acid, DAPI dye, and exonuclease III to the object to be detected; detecting the red fluorescence and blue fluorescence of the object to be detected, and detecting the target antigen by ratio fluorescence method. The ratio fluorescence method of the present invention compares the intensity of the two fluorescences as signal parameters, which is more accurate, more sensitive, and more selective; and improves the sensitivity, overcomes the disadvantages of the traditional method that requires labeled antibodies, and saves the detection cost.

The invention relates to a method for determining the total amount of impurity elements in a natural high-purity quartz mineral, which comprises the following steps of: preparing a laser sheet of the quartz mineral, observing and photographing by using a polarizing microscope, designing an analysis test point on a picture, and carrying out point-by-point analysis and determination on a laser ablation and inductively coupled plasma spectrum according to the design point. Accurate determination of impurity elements of quartz minerals in a natural high-purity quartz raw material is realized, comprehensive analysis and evaluation are carried out, and grade characteristics of the quartz minerals in the high-purity quartz raw material are further determined. The method is suitable for analysis and determination of impurity elements in natural quartz minerals, has the advantages of low detection limit, high sensitivity, wide linear range and the like, can be applied to effective and rapid evaluation, quality monitoring and the like of high-purity quartz raw materials, and provides quartz mineral raw materials with stable grades for the high-purity quartz sand industry.

The invention relates to a qualitative and quantitative method for liensinine perchlorate. The qualitative method comprises the following steps: weighing 1mg of liensinine perchlorate and 1mg of liensinine, uniformly grinding and tabletting the liensinine perchlorate and the liensinine with 100mg of potassium bromide respectively, acquiring an infrared spectrum of 4000-400cm <-1 > by adopting an infrared spectrometer, and comparing spectrograms to find that the peak shapes of the liensinine perchlorate and the liensinine are similar, the peak shapes of the liensinine perchlorate and the liensinine perchlorate are common characteristic peaks and the peak shapes of the liensinine perchlorate are unique characteristic peaks of the liensinine perchlorate and the liensinine are common characteristic peaks and the peak shapes of the The quantitative method comprises the following steps: weighing a series of liensinine perchlorate reference substances with different masses, uniformly grinding and tabletting the liensinine perchlorate reference substances and 100mg of potassium bromide respectively, collecting an infrared spectrum, carrying out integration on a 625cm <-1 > characteristic peak to obtain a peak area, and drawing a standard curve by taking the peak area as a vertical coordinate and taking the sampling mass of the reference substances as a horizontal coordinate; the method comprises the following steps: weighing a sample to be detected, collecting an infrared spectrum, integrating a 625cm <-1 > characteristic peak to obtain a peak area, substituting the peak area into the standard curve equation, calculating the mass of the liensinine perchlorate in the sample, and substituting the mass into the content calculation formula to obtain the content of the liensinine perchlorate in the sample. The method is good in precision, repeatability and stability, wide in linear range, high in linear correlation coefficient and high in recovery rate, the problem that liensinine interference cannot be avoided in an existing content determination method is effectively solved, whether liensinine perchlorate is generated or not can be qualitatively judged, and the quantity of liensinine perchlorate generated can be quantitatively detected; and a feasible new method is provided for production process optimization and quality evaluation of liensinine perchlorate.

This invention discloses a method for quantitatively detecting muscle inositol in blood. It employs an electrochemical method and specifically includes the following steps: a) preparation of a series of standard working solutions; b) preparation of a blank sample solution; c) plotting a standard curve using differential pulse voltammetry (DPV) with the blank sample solution and the series of standard working solutions; d) preparation of the test sample solution; e) determination of the muscle inositol content in the test sample. Experiments have demonstrated that the method of this invention yields accurate and reliable results and can be used for clinical blood testing.

The invention provides a surface plasma resonance chip for detecting tetanus antibody concentration in serum and a preparation method and application thereof, and belongs to the technical field of medicine detection. The chip for detecting the tetanus antibody concentration in the serum, provided by the invention, is a surface plasma resonance chip coupled with tetanus toxoid, and the coupling level of the tetanus toxoid is 1,000 RU to 10,000 RU. According to the chip and the detection method provided by the invention, the concentration level of the tetanus antibody in a serum sample can be specifically and quantitatively detected, the detection sensitivity is high, the linear range is wide, the precision and the accuracy are good, and the requirements of quantitative analysis of biological samples can be met.

The invention discloses an AuPdNPs (at) GO-MWCNTs composite material modified immunosensor with a wide detection range and application of the AuPdNPs (at) GO-MWCNTs composite material modified immunosensor, the composite material is prepared by dispersing graphene oxide and carboxylated MWCNTs in a solution, performing an ultrasonic reaction in an ice-water bath to obtain a GO-MWCNTs compound, then dropwise adding an AuPdNPs solution onto a GO-MWCNTs film, and after the AuPdNPs solution is air-dried, forming the AuPdNPs (at) GO-MWCNTs composite material. The detection limit of the immunosensor modified by the AuPdNPs (at) GO-MWCNTs compound on lipoprotein phospholipase A2 is 0.3 ng / ml, and the detection range is 1 ng / ml to 2 [mu] g / ml; and the method has good specificity, the repeatability, the stability and the clinical property all meet the requirements, and the content of the lipoprotein phospholipase A2 in a clinical sample can be quantitatively detected.

The invention discloses a preparation method and application of a carboxyl functionalized MON (at) ZIF-8-NH2 solid phase microextraction material. The preparation method comprises the following steps: taking ZIF-8-NH2 as an inner core and MON-2COOH as a shell to obtain a carboxyl-functionalized MON-coated ZIF-8-NH2 material, then fixing the carboxyl-functionalized MON-coated ZIF-8-NH2 material on a substrate by adopting a physical coating technology to obtain a solid-phase micro-extraction coating fiber based on carboxyl-functionalized MON-coated ZIF-8-NH2, and using the extraction fiber to measure hydroxyl polycyclic aromatic hydrocarbon in urine. The solid-phase micro-extraction coating fiber disclosed by the invention is thinner in coating, better in extraction effect, longer in service life and higher in stability. The method can be used for extracting various hydroxyl polycyclic aromatic hydrocarbons in urine at the same time by being combined with liquid chromatography-mass spectrometry, and has good stability, meanwhile, the determination method has excellent accuracy, and the recovery rate and the detection limit meet the detection requirements of trace analysis.

The present invention provides a weakly conductive amino acid molecular imprinting polymer film electrochemical sensor and a preparation method thereof, and a ketamine detection method, belonging to the technical field of drug detection. The present invention uses amino acids as functional monomers and ketamine (KT) as template molecules to synthesize weakly conductive amino acids @KT-MIPs by electropolymerization. Due to the rich active sites and good electrochemical activity of amino acids, the polyamino acid film polymerized on the electrode surface exhibits excellent catalytic activity and biocompatibility. The polyamino acid film not only combines ketamine as a template molecule, but also improves the electrocatalytic performance of the ketamine detection electrode, and has specific recognition of ketamine, which can improve the accuracy of the detection results.

The invention belongs to the technical field of magnetic solid-phase extraction and analysis. The invention provides a method for quantitatively detecting bisphenol pollutants in traditional Chinese medicines for soil and roots based on a magnetic MOF (at) COF porous composite material combined with LC-MSMS. The method comprises the steps of preparation of the magnetic MOF (at) COF porous composite material, sample pretreatment and LC-MSMS detection and quantitative analysis. The magnetic MOF (COF) porous composite material disclosed by the invention has rich hierarchical pore structures and high specific surface area, not only retains regular pore channels and active sites of a metal organic framework (MOF), but also is connected with a covalent organic framework (COF), so that the stability of the material is greatly improved. Meanwhile, the material is mild in preparation condition, low in cost and excellent in adsorption performance. Compared with the existing method, the detection method provided by the invention has the advantages of more thorough extraction, lower detection limit, higher recovery rate, simpler operation and lower cost, and is suitable for two matrixes of soil and root system traditional Chinese medicines.