1694results about How to "Wide linear range" patented technology

A method for determining chemical oxygen demand of a water sample comprises the steps of (a) applying a constant potential bias to a photoelectrochemical cell, having a photoactive working electrode (e.g. a layer of titanium dioxide nanoparticles coated on an inert conductive substrate) and a counter electrode, and containing a supporting electrolyte solution; (b) illuminating the working electrode with a light source and recording the background photocurrent produced at the working electrode from the supporting electrolyte solution; (c) adding a water sample, to be analyzed, to the photoelectrochemical cell; (d) illuminating the working electrode with a light source and recording the total photoelectrocurrent produced with the sample; (e) determining the chemical oxygen demand according to the type (exhaustive or non-exhaustive) of degradation conditions employed.

The invention discloses a method for detecting arc faults based on the time-frequency characteristics of high-frequency current component. The method comprises the following steps: measuring the transient high-frequency current in a primary loop by using a self-integrating Rogowski coil and performing the spectrum analysis of the high-frequency current; measuring the time difference between the zero-crossing time of a power-frequency current and the time that the signal of the transient high-frequency current appears by adopting a counting method via using an interrupt trigger and a timer/counter, so as to confirm the phase angle position where the high-frequency current occurs in the protected loop load current; and judging whether an arc fault is caused or not according to the spectrum characteristics of the high-frequency current, the phase angle position where the high-frequency current signal occurs and regularity of the signal of the high-frequency current. The invention is capable of detecting arc faults in the normal power supply, is limited by installation position slightly, can distinguish the transient current generated by normally connecting/disconnecting a circuit from the power electronic-load current of the switch power supply and the like, and reduces and avoids misoperation.

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention discloses a needle amperometric determination type glucose sensor for subcutaneous tissue real-time monitoring and manufacturing method thereof. The sensor comprises a needle reference electrode (2) and at least one needle working electrode (1) including a conducting layer (8) and an enzyme membrane layer (6), and is characterized in that the working electrode (1) further comprises a polymer material inner membrane layer (7) and a polymer material control diffusion layer (5), and the conducting layer (8), the polymer material inner membrane layer (7), the enzyme membrane layer (6) and the polymer material control diffusion layer (5) are coated in turn from inner to outside. According to the manufacturing method in the invention, the glucose sensor has good stability, high flexibility, wide linear range of output current and glucose concentration, short response time, continuous real-time monitoring, good fastness and stability of enzyme bonding, high flexibility, selectivity and repeatability.

The invention relates to composite aerogel of graphene/Prussian-blue complexes, and a preparation method and application thereof. The composite aerogel is in a three-dimensional network structure formed by graphene sheets. Particles of the Prussian-blue complexes are nano-scaled and attach to the graphene sheets. The preparation method of the composite aerogel includes adding metallic cyanide in graphene oxide suspension, allowing for dispersion to obtain suspension A, dissolving metal salt in aqueous solution containing reductant to obtain solution B, ultrasonically mixing the suspension A and the solution B, allowing for standing, and drying to obtain the composite aerogel. The composite aerogel is large in specific surface area and low in density and is highly conductive. The preparation method of the composite aerogel is simple to operate, low in cost and applicable to large-scale industrial production. The composite aerogel has excellent hydrogen storage performance, hydrogen peroxide catalytic reduction and electrochromic property.

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I/creatine kinase isoenzyme/myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention discloses a kit for detecting alpha 1-microglobulin and a preparation method of the kit. The kit consists of a reagent R1 and a reagent R2 according to the volume ratio of 1: 1. The reagent R1 comprises 120-150 mmol/L of a buffer solution, 3-20 g/L of a stabilizer, 5-20 g/L of electrolyte, 3-20 g/L of a sensitizer, 0.5-10 g/L of a surfactant, 0.2-1 g/L of a preservative and purified water. The reagent R2 comprises 1.5-3.5 g/L of latex microspheres with two grain sizes, 20-150 mmol/L of a suffer solution, 3-20 g/L of a stabilizer, 5-20 g/L of electrolyte, 0.5 g/L of a preservative and purified water, wherein the latex microspheres with two grain sizes are respectively coupled with the same anti-human alpha 1-microglobulin polyclonal antibody. The pH value of the kit is 7.0-9.5. The kit is rapid in detection and high in accuracy. The production cost is greatly reduced. The kit is suitable for industrial production and has high application value.

The invention relates to a doped nano sensitive material for monitoring benzene, which is characterized in that: the doped nano sensitive material consists of Ag atom-doped TiO2, Fe2O3 and Al2O3 powder, wherein the content ranges of the components are that: the content range of Ag is 10 to 12 percent, the content range of TiO2 is 30 to 40 percent, the content range of Fe2O3 is 25 to 35 percent, and the content range of Al2O3 is 15 to 25 percent; and the grain diameter is less than 30nm. Benzene sensors prepared from the sensitive material provided by the invention have wide linear range, goodselectivity and high sensitivity, can monitor micro benzene in the air on line, and are not influenced by coexisting substances.

The invention relates to a magnetic particle chemiluminescence double-layer micro-fluidic chip used for whole-blood sample detection. The micro-fluidic chip comprises a top plate (1) and a bottom plate (2). The top plate comprises a sample feeding port (4), a sample filling region (12), a marked ligand storage pool (5) and a sample mixing region (13). The bottom plate comprises a filtering region (6), a magnetic particle coating region (7), a cleaning region (14), a detecting region (8), a cleaning fluid storage pool (9), a luminous substrate liquid storage pool (10) and a liquid release channel (16).

System, method, and test strip for solid phase, electrochemical, quantitative analysis of analytes contained in biological fluid samples. Preliminary to analysis, a test sample solution can be applied to a sample collection pad associated with the solid phase test environment of the test strip. The test sample solution and a test kit reagent are thereby initially contacted, under assay conditions, within this solid phase test environment, and caused to migrate along a fluid pathway therein. Irrespective of the assay format (competitive assay, sandwich assay, etc.), a test kit reagent (e.g. labeled substance) and the analyte of interest (e.g. proteins, hormones or enzymes, small molecules, polysaccharides, antibodies, nucleic acids, drugs, toxins, viruses or virus particles, portions of a cell wall and other compounds which have specific or characteristic markers that permit their identification), either interact with one another to form a complex, or, alternatively, compete with one another for interaction with another test kit reagent, resulting in the concentration of an indicator substance within a delimited area of the solid phase. Thereafter, the delimited area of the test strip is subjected to electrochemical analysis and the results determined by monitoring an electrochemical transition in the form of an indicator, or derivative of the indicator (e.g. indicator species), by potentiostatic or potentiometric quantitative analysis (e.g. anodic stripping voltammetry). This electrochemical transition of the indicator has a characteristic electrical fingerprint that can be measured and which, when compared to a standard, can be correlated with the concentration of the analyte in the sample. This method is suitable for the determination/monitoring of therapeutic range of drugs (anti-convulsants drugs), determination of critical and potentially dangerous levels of endogenous materials which are indicative of disease states (prostate cancer), and numerous other applications presently requiring elaborate and time-consuming clinical laboratory analysis.

The invention discloses a fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and a reagent kit thereof. The fluorescent immunochromatography method for the whole quantitative detection of the C-reactive protein (CRP) utilizes excellent fluorescent characteristics of quantum dots, and combines double-color marking technology and immunochromatography technology to achieve fluorescent quantitative detection on the basis of optimizing each constituent elements of test paper. Compared with a conventional colloidal gold immunochromatography method, the fluorescent immunochromatography method for the whole quantitative detection of the CRP has the advantages of being good in stability, low in non-specificity, high in flexibility, wide in linear range and accurate in quantifying. The reagent kit of the fluorescent immunochromatography method can perform the whole quantifying and can simultaneously predict and evaluate infectious diseases, antibiotic effects and cardiovascular and cerebrovascular diseases. The fluorescent immunochromatography method for the whole quantitative detection of the CRP and the reagent kit of the fluorescent immunochromatography method are suitable for various-level hospitals, and particularly contribute to wide popularization in basic-level hospitals and clinics.

The invention relates to a magnetic particle direct chemiluminiscence microfluidic chip for whole blood sample testing. The chip is composed of a top adhesive tape (10), a chip substrate (1) and a bottom adhesive tape (13); the chip substrate comprises a filtering area (2), a coating area (3), a cleaning area (4), a testing area (5), a cleaning liquid storing pool (7), a luminescence substrate liquid storing pool (8) and a waste liquid pool (11), wherein the testing area is connected with the cleaning liquid storing pool and the luminescence substrate liquid storing pool through liquid releasing channels (9) respectively; the top adhesive tape comprises a sample feeding opening (11) and a cleaning liquid storing pool demising hole (12).

The invention discloses a magnetic particle chemiluminescence micro-fluidic chip used for whole-blood sample detection. The chip is composed of top adhesive tape (12), a chip substrate (1) and bottom adhesive tape (15), wherein a filtering area (2), a coating area (3), a reacting area (5), a cleaning area (6), a detecting area (7) and liquid release channels (8) on the chip substrate are sequentially connected, a marking ligand storage pool (4) on the chip substrate is connected with the reacting area (5), the detecting area (7) is connected with a cleaning liquid storage pool (9) and a light-emitting substrate liquid storage pool (10) through the liquid release channels (8), and the top adhesive tape comprises a sample adding port (13). Magnetic particle marking ligands are coated with the coating area (3). The marking ligand storage pool (4) is used for storing enzyme or light-emitting agent marking ligands.

The invention discloses a method for applying an ionic liquid functionalized graphene modified electrode in concurrent detection of 5-hydroxytryptamine and dopamine, wherein an amine terminated imidazole ionic liquid and the epoxy group of graphene oxide lamellas are subjected to a nucleophilic ring opening reaction to form ionic liquid functionalized graphene, and then the ionic liquid functionalized graphene is modified on a glassy carbon electrode to form a modification layer so as to concurrently detecting 5-hydroxytryptamine and dopamine. According to the present invention, research results show that the ionic liquid functionalized graphene modified electrode provides good electrocatalysis activity for oxidation of 5-hydroxytryptamine and dopamine, and can be provided for individually detecting 5-hydroxytryptamine and dopamine and concurrently detecting 5-hydroxytryptamine and dopamine, and the method has characteristics of simple detection process, high sensitivity, rapidness and convenience.

The invention discloses a design and manufacture technology of a sensing unit of a sensor and one or more types of sensor chips consisting of the sensing unit, in particular to one or more types of sensor chips capable of detecting the magnetic field parallel and vertical to the surface of the chip and the acceleration of an object. The invention has the advantages that the process is simple and easy, the sensitivity is high, and batched production is easily realized; the sensing unit of the sensor comprises a wafer substrate, a seed layer, a soft magnetic material layer, a conducting layer and a N-layer structure; the conducting layer is wrapped by the soft magnetic material layer; the N-layer structure is formed by combination of the soft magnetic material layer and the conducting layer; and simultaneously a bent structure is formed by N long lines in the plane (the sensing unit is a single-strip-shaped structure when N is equal to 1). The sensing unit of the sensor realizes single-axis, double-axis and three-axis magnetic field detection by different packaging forms. Simultaneously, due to the combination of the sensing unit of the sensor and a cantilever structure with magnetic mass blocks, the single-axis, double-axis and three-axis acceleration detection can be realized.